Search Menu
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search Scholarly Publications

Title: Multi-omic dynamics associate oxygenic photosynthesis with nitrogenase-mediated H2 production in Cyanothece sp. ATCC 51142

Abstract

This study combines transcriptomic and proteomic profiling to provide new insights on the metabolic relationship between oxygenic photosynthesis and nitrogenase-mediated H2 production in the model cyanobacterium, Cyanothece sp. ATCC 51142. To date, the proposed mechanisms used to describe the energy metabolism processes that support H2 production in Cyanothece 51142 have assumed that ATP and reductant requirements are derived solely from glycogen oxidation and/or cyclic-electron flow around photosystem I. The results from this study present and test an alternative hypothesis by showing that net-positive rates of oxygenic photosynthesis and increased expression of photosystem II reaction centers correspond and are synchronized with nitrogenase expression and H2 production. These findings provide a new and more complete view on the metabolic processes contributing to the energy budget of photosynthetic H2 production and highlight the likely role of photocatalytic H2O oxidation as a major participating process.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1]
+ Show Author Affiliations
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1228337
Report Number(s):
PNNL-SA-111925
Journal ID: ISSN 2045-2322; srep16004
Grant/Contract Number:  
AC05-76RLO1830
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 5; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; multi-omic; oxygenic photosynthesis; nitrogenase-mediated; Environmental Molecular Sciences Laboratory; applied microbiology; microbiology; proteomics; systems biology

Citation Formats

Bernstein, Hans C., Charania, Moiz A., McClure, Ryan S., Sadler, Natalie C., Melnicki, Matthew R., Hill, Eric A., Markillie, Lye Meng, Nicora, Carrie D., Wright, Aaron T., Romine, Margaret F., and Beliaev, Alexander S. Multi-omic dynamics associate oxygenic photosynthesis with nitrogenase-mediated H2 production in Cyanothece sp. ATCC 51142. United States: N. p., 2015. Web. doi:10.1038/srep16004.
Copy to clipboard
Bernstein, Hans C., Charania, Moiz A., McClure, Ryan S., Sadler, Natalie C., Melnicki, Matthew R., Hill, Eric A., Markillie, Lye Meng, Nicora, Carrie D., Wright, Aaron T., Romine, Margaret F., & Beliaev, Alexander S. Multi-omic dynamics associate oxygenic photosynthesis with nitrogenase-mediated H2 production in Cyanothece sp. ATCC 51142. United States. https://doi.org/10.1038/srep16004
Copy to clipboard
Bernstein, Hans C., Charania, Moiz A., McClure, Ryan S., Sadler, Natalie C., Melnicki, Matthew R., Hill, Eric A., Markillie, Lye Meng, Nicora, Carrie D., Wright, Aaron T., Romine, Margaret F., and Beliaev, Alexander S. Tue . "Multi-omic dynamics associate oxygenic photosynthesis with nitrogenase-mediated H2 production in Cyanothece sp. ATCC 51142". United States. https://doi.org/10.1038/srep16004. https://www.osti.gov/servlets/purl/1228337.
Copy to clipboard
@article{osti_1228337,
title = {Multi-omic dynamics associate oxygenic photosynthesis with nitrogenase-mediated H2 production in Cyanothece sp. ATCC 51142},
author = {Bernstein, Hans C. and Charania, Moiz A. and McClure, Ryan S. and Sadler, Natalie C. and Melnicki, Matthew R. and Hill, Eric A. and Markillie, Lye Meng and Nicora, Carrie D. and Wright, Aaron T. and Romine, Margaret F. and Beliaev, Alexander S.},
abstractNote = {This study combines transcriptomic and proteomic profiling to provide new insights on the metabolic relationship between oxygenic photosynthesis and nitrogenase-mediated H2 production in the model cyanobacterium, Cyanothece sp. ATCC 51142. To date, the proposed mechanisms used to describe the energy metabolism processes that support H2 production in Cyanothece 51142 have assumed that ATP and reductant requirements are derived solely from glycogen oxidation and/or cyclic-electron flow around photosystem I. The results from this study present and test an alternative hypothesis by showing that net-positive rates of oxygenic photosynthesis and increased expression of photosystem II reaction centers correspond and are synchronized with nitrogenase expression and H2 production. These findings provide a new and more complete view on the metabolic processes contributing to the energy budget of photosynthetic H2 production and highlight the likely role of photocatalytic H2O oxidation as a major participating process.},
doi = {10.1038/srep16004},
journal = {Scientific Reports},
number = ,
volume = 5,
place = {United States},
year = {Tue Nov 03 00:00:00 EST 2015},
month = {Tue Nov 03 00:00:00 EST 2015}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1038/srep16004
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Citation Metrics:
Cited by: 9 works
Citation information provided by
Web of Science
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

Fermentative and Photochemical Production of Hydrogen in Algae
journal, November 1942

Oxygen Sensitivity of Algal H2-Production
book, January 1997

Sustained Photobiological Hydrogen Gas Production upon Reversible Inactivation of Oxygen Evolution in the Green Alga Chlamydomonas reinhardtii
journal, January 2000

  • Melis, Anastasios; Zhang, Liping; Forestier, Marc
  • Plant Physiology, Vol. 122, Issue 1
  • DOI: 10.1104/pp.122.1.127

Biohydrogen production: prospects and limitations to practical application
journal, February 2004

Unicellular, aerobic nitrogen-fixing cyanobacteria of the genus Cyanothece.
journal, March 1993

Hydrogen production by cyanobacteria in an automated outdoor photobioreactor under aerobic conditions
journal, October 2002

  • Tsygankov, A. A.; Fedorov, A. S.; Kosourov, S. N.
  • Biotechnology and Bioengineering, Vol. 80, Issue 7
  • DOI: 10.1002/bit.10431

High rates of photobiological H2 production by a cyanobacterium under aerobic conditions
journal, December 2010

  • Bandyopadhyay, Anindita; Stöckel, Jana; Min, Hongtao
  • Nature Communications, Vol. 1, Issue 1
  • DOI: 10.1038/ncomms1139

Nitrogenase-Dependent Hydrogen Production by Cyanobacteria
book, January 2014

Hydrogen Photoproduction by Immobilized N 2 -Fixing Cyanobacteria: Understanding the Role of the Uptake Hydrogenase in the Long-Term Process
journal, July 2014

  • Kosourov, Sergey; Leino, Hannu; Murukesan, Gayathri
  • Applied and Environmental Microbiology, Vol. 80, Issue 18
  • DOI: 10.1128/AEM.01776-14

Sustained photobiological hydrogen production in the presence of N2 by nitrogenase mutants of the heterocyst-forming cyanobacterium Anabaena
journal, November 2014

  • Masukawa, Hajime; Sakurai, Hidehiro; Hausinger, Robert P.
  • International Journal of Hydrogen Energy, Vol. 39, Issue 34
  • DOI: 10.1016/j.ijhydene.2014.09.090

Proteome Analyses of Strains ATCC 51142 and PCC 7822 of the Diazotrophic Cyanobacterium Cyanothece sp. under Culture Conditions Resulting in Enhanced H 2 Production
journal, November 2012

  • Aryal, Uma K.; Callister, Stephen J.; Mishra, Sujata
  • Applied and Environmental Microbiology, Vol. 79, Issue 4
  • DOI: 10.1128/AEM.02864-12

Variations in the Rhythms of Respiration and Nitrogen Fixation in Members of the Unicellular Diazotrophic Cyanobacterial Genus Cyanothece
journal, December 2012

  • Bandyopadhyay, Anindita; Elvitigala, Thanura; Liberton, Michelle
  • Plant Physiology, Vol. 161, Issue 3
  • DOI: 10.1104/pp.112.208231

The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle
journal, September 2008

  • Welsh, E. A.; Liberton, M.; Stockel, J.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 39
  • DOI: 10.1073/pnas.0805418105

Genome-Scale Modeling of Light-Driven Reductant Partitioning and Carbon Fluxes in Diazotrophic Unicellular Cyanobacterium Cyanothece sp. ATCC 51142
journal, April 2012

Metabolic Rhythms of the Cyanobacterium Cyanothece sp. ATCC 51142 Correlate with Modeled Dynamics of Circadian Clock
journal, July 2009

Oscillating behavior of carbohydrate granule formation and dinitrogen fixation in the cyanobacterium Cyanothece sp. strain ATCC 51142.
journal, March 1994

Constraint-Based Model of Shewanella oneidensis MR-1 Metabolism: A Tool for Data Analysis and Hypothesis Generation
journal, June 2010

Inference of interactions in cyanobacterial–heterotrophic co-cultures via transcriptome sequencing
journal, April 2014

  • Beliaev, Alexander S.; Romine, Margie F.; Serres, Margrethe
  • The ISME Journal, Vol. 8, Issue 11
  • DOI: 10.1038/ismej.2014.69

Computational analysis of bacterial RNA-Seq data
journal, May 2013

  • McClure, Ryan; Balasubramanian, Divya; Sun, Yan
  • Nucleic Acids Research, Vol. 41, Issue 14
  • DOI: 10.1093/nar/gkt444

Live Cell Chemical Profiling of Temporal Redox Dynamics in a Photoautotrophic Cyanobacterium
journal, October 2013

  • Sadler, Natalie C.; Melnicki, Matthew R.; Serres, Margrethe H.
  • ACS Chemical Biology, Vol. 9, Issue 1
  • DOI: 10.1021/cb400769v

Live Cell Chemical Profiling of Temporal Redox Dynamics in a Photoautotrophic Cyanobacterium
journal, October 2013

  • Sadler, Natalie C.; Melnicki, Matthew R.; Serres, Margrethe H.
  • ACS Chemical Biology, Vol. 9, Issue 1
  • DOI: 10.1021/cb400769v

The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle
journal, September 2008

  • Welsh, E. A.; Liberton, M.; Stockel, J.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 39
  • DOI: 10.1073/pnas.0805418105

Metabolic Pathways for Photobiological Hydrogen Production by Nitrogenase- and Hydrogenase-containing Unicellular Cyanobacteria Cyanothece
journal, November 2011

  • Skizim, Nicholas J.; Ananyev, Gennady M.; Krishnan, Anagha
  • Journal of Biological Chemistry, Vol. 287, Issue 4
  • DOI: 10.1074/jbc.m111.302125

Fermentative and Photochemical Production of Hydrogen in Algae
journal, November 1942

Variations in the Rhythms of Respiration and Nitrogen Fixation in Members of the Unicellular Diazotrophic Cyanobacterial Genus Cyanothece
journal, December 2012

  • Bandyopadhyay, Anindita; Elvitigala, Thanura; Liberton, Michelle
  • Plant Physiology, Vol. 161, Issue 3
  • DOI: 10.1104/pp.112.208231

Sustained Photobiological Hydrogen Gas Production upon Reversible Inactivation of Oxygen Evolution in the Green Alga Chlamydomonas reinhardtii
journal, January 2000

  • Melis, Anastasios; Zhang, Liping; Forestier, Marc
  • Plant Physiology, Vol. 122, Issue 1
  • DOI: 10.1104/pp.122.1.127

Hydrogen Production by the Unicellular, Diazotrophic Cyanobacterium Cyanothece sp. Strain ATCC 51142 under Conditions of Continuous Light
journal, May 2010

  • Min, H.; Sherman, L. A.
  • Applied and Environmental Microbiology, Vol. 76, Issue 13
  • DOI: 10.1128/aem.00146-10

Unicellular, aerobic nitrogen-fixing cyanobacteria of the genus Cyanothece.
journal, March 1993

Sustained H2 Production Driven by Photosynthetic Water Splitting in a Unicellular Cyanobacterium
journal, August 2012

  • Melnicki, Matthew R.; Pinchuk, Grigoriy E.; Hill, Eric A.
  • mBio, Vol. 3, Issue 4
  • DOI: 10.1128/mbio.00197-12

Metabolic Rhythms of the Cyanobacterium Cyanothece sp. ATCC 51142 Correlate with Modeled Dynamics of Circadian Clock
journal, July 2009

Constraint-Based Model of Shewanella oneidensis MR-1 Metabolism: A Tool for Data Analysis and Hypothesis Generation
journal, June 2010

    Sort by:
    [ × clear filter / sort ]

    Works referencing / citing this record:

    Indirect Interspecies Regulation: Transcriptional and Physiological Responses of a Cyanobacterium to Heterotrophic Partnership
    journal, March 2017

    Effect of light wavelength on hot spring microbial mat biodiversity
    journal, January 2018

      Sort by:
      [ × clear filter / sort ]

      Similar Records in DOE PAGES and OSTI.GOV collections: