TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching
Abstract
The AAA+ family ATPase TRIP13 is a key regulator of meiotic recombination and the spindle assembly checkpoint, acting on signaling proteins of the conserved HORMA domain family. Here we present the structure of the Caenorhabditis elegans TRIP13 ortholog PCH-2, revealing a new family of AAA+ ATPase protein remodelers. PCH-2 possesses a substrate-recognition domain related to those of the protein remodelers NSF and p97, while its overall hexameric architecture and likely structural mechanism bear close similarities to the bacterial protein unfoldase ClpX. We find that TRIP13, aided by the adapter protein p31(comet), converts the HORMA-family spindle checkpoint protein MAD2 from a signaling-active ‘closed’ conformer to an inactive ‘open’ conformer. We propose that TRIP13 and p31(comet) collaborate to inactivate the spindle assembly checkpoint through MAD2 conformational conversion and disassembly of mitotic checkpoint complexes. A parallel HORMA protein disassembly activity likely underlies TRIP13's critical regulatory functions in meiotic chromosome structure and recombination.
- Authors:
-
- Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, United States
- National Resource for Automated Molecular Microscopy, Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, United States
- Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, United States, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, United States
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH); March of Dimes Foundation; Sidney Kimmel Foundation for Cancer Research
- OSTI Identifier:
- 1182456
- Alternate Identifier(s):
- OSTI ID: 1228260; OSTI ID: 1430284
- Grant/Contract Number:
- R01GM10414; P41GM103393; GM103310; FY14-251
- Resource Type:
- Published Article
- Journal Name:
- eLife
- Additional Journal Information:
- Journal Name: eLife Journal Volume: 4; Journal ID: ISSN 2050-084X
- Publisher:
- eLife Sciences Publications, Ltd.
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Ye, Qiaozhen, Rosenberg, Scott C., Moeller, Arne, Speir, Jeffrey A., Su, Tiffany Y., and Corbett, Kevin D. TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching. United States: N. p., 2015.
Web. doi:10.7554/eLife.07367.
Ye, Qiaozhen, Rosenberg, Scott C., Moeller, Arne, Speir, Jeffrey A., Su, Tiffany Y., & Corbett, Kevin D. TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching. United States. https://doi.org/10.7554/eLife.07367
Ye, Qiaozhen, Rosenberg, Scott C., Moeller, Arne, Speir, Jeffrey A., Su, Tiffany Y., and Corbett, Kevin D. Tue .
"TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching". United States. https://doi.org/10.7554/eLife.07367.
@article{osti_1182456,
title = {TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching},
author = {Ye, Qiaozhen and Rosenberg, Scott C. and Moeller, Arne and Speir, Jeffrey A. and Su, Tiffany Y. and Corbett, Kevin D.},
abstractNote = {The AAA+ family ATPase TRIP13 is a key regulator of meiotic recombination and the spindle assembly checkpoint, acting on signaling proteins of the conserved HORMA domain family. Here we present the structure of the Caenorhabditis elegans TRIP13 ortholog PCH-2, revealing a new family of AAA+ ATPase protein remodelers. PCH-2 possesses a substrate-recognition domain related to those of the protein remodelers NSF and p97, while its overall hexameric architecture and likely structural mechanism bear close similarities to the bacterial protein unfoldase ClpX. We find that TRIP13, aided by the adapter protein p31(comet), converts the HORMA-family spindle checkpoint protein MAD2 from a signaling-active ‘closed’ conformer to an inactive ‘open’ conformer. We propose that TRIP13 and p31(comet) collaborate to inactivate the spindle assembly checkpoint through MAD2 conformational conversion and disassembly of mitotic checkpoint complexes. A parallel HORMA protein disassembly activity likely underlies TRIP13's critical regulatory functions in meiotic chromosome structure and recombination.},
doi = {10.7554/eLife.07367},
journal = {eLife},
number = ,
volume = 4,
place = {United States},
year = {Tue Apr 28 00:00:00 EDT 2015},
month = {Tue Apr 28 00:00:00 EDT 2015}
}
https://doi.org/10.7554/eLife.07367
Web of Science
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