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Title: Functional diversification of maize RNA polymerase IV and V subtypes via alternative catalytic subunits

Unlike nuclear multisubunit RNA polymerases I, II, and III, whose subunit compositions are conserved throughout eukaryotes, plant RNA polymerases IV and V are nonessential, Pol II-related enzymes whose subunit compositions are still evolving. Whereas Arabidopsis Pols IV and V differ from Pol II in four or five of their 12 subunits, respectively, and differ from one another in three subunits, proteomic analyses show that maize Pols IV and V differ from Pol II in six subunits but differ from each other only in their largest subunits. Use of alternative catalytic second subunits, which are nonredundant for development and paramutation, yields at least two sub-types of Pol IV and three subtypes of Pol V in maize. Pol IV/Pol V associations with MOP1, RMR1, AGO121, Zm_DRD1/CHR127, SHH2a, and SHH2b extend parallels between paramutation in maize and the RNA-directed DNA methylation pathway in Arabidopsis.
Authors:
 [1] ;  [2] ;  [2] ;  [3] ;  [4] ;  [4] ;  [5] ;  [2] ;  [3] ;  [6] ;  [2] ;  [4] ;  [3] ;  [1]
  1. Indiana Univ., Bloomington, IN (United States). Dept. of Biology and Dept. of Molecular and Cellular Biochemistry; Indiana Univ., Bloomington, IN (United States). Howard Hughes Medical Inst.
  2. Monsanto Company, Chesterfield, MO (United States)
  3. Univ. of Arizona, Tucson, AZ (United States). BIO5 Inst., Dept. of Plant Sciences
  4. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  5. Florida State Univ., Tallahassee, FL (United States). Dept. of Biological Science; EMBRAPA Genetic Resources and Biotechnology, Brasilia (Brazil)
  6. Florida State Univ., Tallahassee, FL (United States)
Publication Date:
Report Number(s):
PNNL-SA-105690
Journal ID: ISSN 2211-1247; 44640; 48378; KP1704020
Grant/Contract Number:
AC05-76RL01830
Type:
Published Article
Journal Name:
Cell Reports
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2211-1247
Publisher:
Elsevier
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Environmental Molecular Sciences Laboratory
OSTI Identifier:
1227551
Alternate Identifier(s):
OSTI ID: 1208785