Investigation of the chemical interface in the soybean–aphid and rice–bacteria interactions using MALDI-mass spectrometry imaging
Abstract
Mass spectrometry imaging (MSI) is an emerging technology for high-resolution plant biology. It has been utilized to study plant–pest interactions, but limited to the surface interfaces. Here we expand the technology to explore the chemical interactions occurring inside the plant tissues. Two sample preparation methods, imprinting and fracturing, were developed and applied, for the first time, to visualize internal metabolites of leaves in matrix-assisted laser desorption ionization (MALDI)-MSI. This is also the first time nanoparticle-based ionization was implemented to ionize diterpenoid phytochemicals that were difficult to analyze with traditional organic matrices. The interactions between rice–bacterium and soybean–aphid were investigated as two model systems to demonstrate the capability of high-resolution MSI based on MALDI. Localized molecular information on various plant- or pest-derived chemicals provided valuable insight for the molecular processes occurring during the plant–pest interactions. Basically, salicylic acid and isoflavone based resistance was visualized in the soybean–aphid system and antibiotic diterpenoids in rice–bacterium interactions.
- Authors:
-
- Iowa State Univ., Ames, IA (United States); Ames Lab., Ames, IA (United States)
- Iowa State Univ., Ames, IA (United States)
- Shanghai Jiao Tong Univ., Shanghai (China); Iowa State Univ., Ames, IA (United States)
- Publication Date:
- Research Org.:
- Ames Laboratory (AMES), Ames, IA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1227451
- Report Number(s):
- IS-J-8639
Journal ID: ISSN 0003-2700
- Grant/Contract Number:
- 2014-67013-21720; AC02-07CH11358
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Analytical Chemistry
- Additional Journal Information:
- Journal Volume: 87; Journal Issue: 10; Journal ID: ISSN 0003-2700
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Klein, Adam T., Yagnik, Gargey B., Hohenstein, Jessica D., Ji, Zhiyuan, Zi, Jiachen, Reichert, Malinda D., MacIntosh, Gustavo C., Yang, Bing, Peters, Reuben J., Vela, Javier, and Lee, Young Jin. Investigation of the chemical interface in the soybean–aphid and rice–bacteria interactions using MALDI-mass spectrometry imaging. United States: N. p., 2015.
Web. doi:10.1021/acs.analchem.5b00459.
Klein, Adam T., Yagnik, Gargey B., Hohenstein, Jessica D., Ji, Zhiyuan, Zi, Jiachen, Reichert, Malinda D., MacIntosh, Gustavo C., Yang, Bing, Peters, Reuben J., Vela, Javier, & Lee, Young Jin. Investigation of the chemical interface in the soybean–aphid and rice–bacteria interactions using MALDI-mass spectrometry imaging. United States. https://doi.org/10.1021/acs.analchem.5b00459
Klein, Adam T., Yagnik, Gargey B., Hohenstein, Jessica D., Ji, Zhiyuan, Zi, Jiachen, Reichert, Malinda D., MacIntosh, Gustavo C., Yang, Bing, Peters, Reuben J., Vela, Javier, and Lee, Young Jin. Mon .
"Investigation of the chemical interface in the soybean–aphid and rice–bacteria interactions using MALDI-mass spectrometry imaging". United States. https://doi.org/10.1021/acs.analchem.5b00459. https://www.osti.gov/servlets/purl/1227451.
@article{osti_1227451,
title = {Investigation of the chemical interface in the soybean–aphid and rice–bacteria interactions using MALDI-mass spectrometry imaging},
author = {Klein, Adam T. and Yagnik, Gargey B. and Hohenstein, Jessica D. and Ji, Zhiyuan and Zi, Jiachen and Reichert, Malinda D. and MacIntosh, Gustavo C. and Yang, Bing and Peters, Reuben J. and Vela, Javier and Lee, Young Jin},
abstractNote = {Mass spectrometry imaging (MSI) is an emerging technology for high-resolution plant biology. It has been utilized to study plant–pest interactions, but limited to the surface interfaces. Here we expand the technology to explore the chemical interactions occurring inside the plant tissues. Two sample preparation methods, imprinting and fracturing, were developed and applied, for the first time, to visualize internal metabolites of leaves in matrix-assisted laser desorption ionization (MALDI)-MSI. This is also the first time nanoparticle-based ionization was implemented to ionize diterpenoid phytochemicals that were difficult to analyze with traditional organic matrices. The interactions between rice–bacterium and soybean–aphid were investigated as two model systems to demonstrate the capability of high-resolution MSI based on MALDI. Localized molecular information on various plant- or pest-derived chemicals provided valuable insight for the molecular processes occurring during the plant–pest interactions. Basically, salicylic acid and isoflavone based resistance was visualized in the soybean–aphid system and antibiotic diterpenoids in rice–bacterium interactions.},
doi = {10.1021/acs.analchem.5b00459},
journal = {Analytical Chemistry},
number = 10,
volume = 87,
place = {United States},
year = {Mon Apr 27 00:00:00 EDT 2015},
month = {Mon Apr 27 00:00:00 EDT 2015}
}
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