skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Down-regulation of p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H) and cinnamate 4-hydroxylase (C4H) genes in the lignin biosynthetic pathway of Eucalyptus urophylla x E. grandis leads to improved sugar release

Abstract

In this study, lignocellulosic materials provide an attractive replacement for food-based crops used to produce ethanol. Understanding the interactions within the cell wall is vital to overcome the highly recalcitrant nature of biomass. One factor imparting plant cell wall recalcitrance is lignin, which can be manipulated by making changes in the lignin biosynthetic pathway. In this study, eucalyptus down-regulated in expression of cinnamate 4-hydroxylase (C4H, EC 1.14.13.11) or p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H, EC 1.14.13.36) were evaluated for cell wall composition and reduced recalcitrance.

Authors:
 [1];  [1];  [2];  [2];  [2];  [3];  [1];  [1];  [1];  [2];  [1]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  2. ArborGen Inc. Ridgeville, SC (United States)
  3. Michigan State Univ., East Lansing, MI (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1225915
Report Number(s):
NREL/JA-5100-62715
Journal ID: ISSN 1754-6834
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 8; Journal Issue: 1; Related Information: Biotechnology for Biofuels; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Eucalyptus urophylla x E. grandis; recalcitrance; genetic modification; lignin biosynthesis; pretreatment

Citation Formats

Sykes, Robert W., Gjersing, Erica L., Foutz, Kirk, Rottmann, William H., Kuhn, Sean A., Foster, Cliff E., Ziebell, Angela, Turner, Geoffrey B., Decker, Stephen R., Hinchee, Maud A. W., and Davis, Mark F. Down-regulation of p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H) and cinnamate 4-hydroxylase (C4H) genes in the lignin biosynthetic pathway of Eucalyptus urophylla x E. grandis leads to improved sugar release. United States: N. p., 2015. Web. doi:10.1186/s13068-015-0316-x.
Sykes, Robert W., Gjersing, Erica L., Foutz, Kirk, Rottmann, William H., Kuhn, Sean A., Foster, Cliff E., Ziebell, Angela, Turner, Geoffrey B., Decker, Stephen R., Hinchee, Maud A. W., & Davis, Mark F. Down-regulation of p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H) and cinnamate 4-hydroxylase (C4H) genes in the lignin biosynthetic pathway of Eucalyptus urophylla x E. grandis leads to improved sugar release. United States. doi:10.1186/s13068-015-0316-x.
Sykes, Robert W., Gjersing, Erica L., Foutz, Kirk, Rottmann, William H., Kuhn, Sean A., Foster, Cliff E., Ziebell, Angela, Turner, Geoffrey B., Decker, Stephen R., Hinchee, Maud A. W., and Davis, Mark F. Thu . "Down-regulation of p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H) and cinnamate 4-hydroxylase (C4H) genes in the lignin biosynthetic pathway of Eucalyptus urophylla x E. grandis leads to improved sugar release". United States. doi:10.1186/s13068-015-0316-x. https://www.osti.gov/servlets/purl/1225915.
@article{osti_1225915,
title = {Down-regulation of p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H) and cinnamate 4-hydroxylase (C4H) genes in the lignin biosynthetic pathway of Eucalyptus urophylla x E. grandis leads to improved sugar release},
author = {Sykes, Robert W. and Gjersing, Erica L. and Foutz, Kirk and Rottmann, William H. and Kuhn, Sean A. and Foster, Cliff E. and Ziebell, Angela and Turner, Geoffrey B. and Decker, Stephen R. and Hinchee, Maud A. W. and Davis, Mark F.},
abstractNote = {In this study, lignocellulosic materials provide an attractive replacement for food-based crops used to produce ethanol. Understanding the interactions within the cell wall is vital to overcome the highly recalcitrant nature of biomass. One factor imparting plant cell wall recalcitrance is lignin, which can be manipulated by making changes in the lignin biosynthetic pathway. In this study, eucalyptus down-regulated in expression of cinnamate 4-hydroxylase (C4H, EC 1.14.13.11) or p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H, EC 1.14.13.36) were evaluated for cell wall composition and reduced recalcitrance.},
doi = {10.1186/s13068-015-0316-x},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 8,
place = {United States},
year = {2015},
month = {8}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 12 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Ultrastructure and Mechanical Properties of Populus Wood with Reduced Lignin Content Caused by Transgenic Down-Regulation of Cinnamate 4-Hydroxylase
journal, September 2010

  • Bjurhager, Ingela; Olsson, Anne-Mari; Zhang, Bo
  • Biomacromolecules, Vol. 11, Issue 9
  • DOI: 10.1021/bm100487e

Genetic and biotechnological approaches for biofuel crop improvement
journal, April 2010


Cellulase digestibility of pretreated biomass is limited by cellulose accessibility
journal, January 2007

  • Jeoh, Tina; Ishizawa, Claudia I.; Davis, Mark F.
  • Biotechnology and Bioengineering, Vol. 98, Issue 1, p. 112-122
  • DOI: 10.1002/bit.21408

Lignin content in natural Populus variants affects sugar release
journal, March 2011

  • Studer, M. H.; DeMartini, J. D.; Davis, M. F.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 15, p. 6300-6305
  • DOI: 10.1073/pnas.1009252108

What is (and is not) vital to advancing cellulosic ethanol
journal, April 2007


Variation in content and composition of lignin in young wood of Norway spruce
journal, March 2004

  • Wadenbäck, J.; Clapham, D.; Gellerstedt, G.
  • Holzforschung, Vol. 58, Issue 2
  • DOI: 10.1515/HF.2004.015

Molecular characterization of the pyrolysis of biomass
journal, March 1987


Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass
journal, February 2011

  • Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808
  • DOI: 10.1073/pnas.1100310108

Lignin modification improves fermentable sugar yields for biofuel production
journal, June 2007

  • Chen, Fang; Dixon, Richard A.
  • Nature Biotechnology, Vol. 25, Issue 7, p. 759-761
  • DOI: 10.1038/nbt1316

NMR Characterization of C3H and HCT Down-Regulated Alfalfa Lignin
journal, October 2009


Identification and Characterization of Four Missense Mutations in Brown midrib 12 (Bmr12), the Caffeic O-Methyltranferase (COMT) of Sorghum
journal, April 2012


Lignin: Genetic Engineering and Impact on Pulping
journal, January 2003

  • Baucher, Marie; Halpin, Claire; Petit-Conil, Michel
  • Critical Reviews in Biochemistry and Molecular Biology, Vol. 38, Issue 4, p. 305-350
  • DOI: 10.1080/10409230391036757

Designed for deconstruction - poplar trees altered in cell wall lignification improve the efficacy of bioethanol production
journal, January 2012


ORIGINAL RESEARCH: Lignocellulose recalcitrance screening by integrated high-throughput hydrothermal pretreatment and enzymatic saccharification
journal, April 2010

  • Selig, Michael J.; Tucker, Melvin P.; Sykes, Robert W.
  • Industrial Biotechnology, Vol. 6, Issue 2
  • DOI: 10.1089/ind.2010.0009

Effects of Coumarate 3-Hydroxylase Down-regulation on Lignin Structure
journal, January 2006

  • Ralph, John; Akiyama, Takuya; Kim, Hoon
  • Journal of Biological Chemistry, Vol. 281, Issue 13, p. 8843-8853
  • DOI: 10.1074/jbc.M511598200

Engineering secondary cell wall deposition in plants
journal, November 2012

  • Yang, Fan; Mitra, Prajakta; Zhang, Ling
  • Plant Biotechnology Journal, Vol. 11, Issue 3
  • DOI: 10.1111/pbi.12016

Fuel from plant cell walls: recent developments in second generation bioethanol research
journal, June 2011

  • Cook, Charis; Devoto, Alessandra
  • Journal of the Science of Food and Agriculture, Vol. 91, Issue 10
  • DOI: 10.1002/jsfa.4455

Advances in modifying lignin for enhanced biofuel production
journal, June 2010

  • Simmons, Blake A.; Loqué, Dominique; Ralph, John
  • Current Opinion in Plant Biology, Vol. 13, Issue 3, p. 312-319
  • DOI: 10.1016/j.pbi.2010.03.001

Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa (Medicago sativa L.)
journal, November 2005

  • Reddy, M. S. S.; Chen, F.; Shadle, G.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 46
  • DOI: 10.1073/pnas.0505749102

Increase in 4-Coumaryl Alcohol Units during Lignification in Alfalfa ( Medicago sativa ) Alters the Extractability and Molecular Weight of Lignin
journal, October 2010

  • Ziebell, Angela; Gracom, Kristen; Katahira, Rui
  • Journal of Biological Chemistry, Vol. 285, Issue 50
  • DOI: 10.1074/jbc.M110.137315

Phytozome: a comparative platform for green plant genomics
journal, November 2011

  • Goodstein, David M.; Shu, Shengqiang; Howson, Russell
  • Nucleic Acids Research, Vol. 40, Issue D1
  • DOI: 10.1093/nar/gkr944

Trends in biotechnological production of fuel ethanol from different feedstocks
journal, September 2008


Genetic variation of wood chemical traits and association with underlying genes in Eucalyptus urophylla
journal, March 2013


Genome-wide analysis of the lignin toolbox of Eucalyptus grandis
journal, February 2015

  • Carocha, Victor; Soler, Marçal; Hefer, Charles
  • New Phytologist, Vol. 206, Issue 4
  • DOI: 10.1111/nph.13313

Can genetic engineering of lignin deposition be accomplished without an unacceptable yield penalty?
journal, April 2013


Improvement of fermentable sugar yields of mangium through transgenic overexpression of xyloglucanase
journal, December 2011


Primer3—new capabilities and interfaces
journal, June 2012

  • Untergasser, Andreas; Cutcutache, Ioana; Koressaar, Triinu
  • Nucleic Acids Research, Vol. 40, Issue 15
  • DOI: 10.1093/nar/gks596

Effects on Lignin Structure of Coumarate 3-Hydroxylase Downregulation in Poplar
journal, May 2012


Two High-Throughput Techniques for Determining Wood Properties as Part of a Molecular Genetics Analysis of Hybrid Poplar and Loblolly Pine
journal, January 1999

  • Tuskan, Gerald; West, Darrell; Bradshaw, Harvy D.
  • Applied Biochemistry and Biotechnology, Vol. 77, Issue 1-3
  • DOI: 10.1385/ABAB:77:1-3:55

High-Throughput Screening Techniques for Biomass Conversion
journal, October 2009


Reduction of Lignin Content by Suppression of Expression of the LIM Domain Transcription Factor in Eucalyptus camaldulensis
journal, December 2006


The genome of Eucalyptus grandis
journal, June 2014

  • Myburg, Alexander A.; Grattapaglia, Dario; Tuskan, Gerald A.
  • Nature, Vol. 510, Issue 7505
  • DOI: 10.1038/nature13308

    Works referencing / citing this record:

    Lignin modification in planta for valorization
    journal, January 2018