skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Nobel Lecture: Single-molecule spectroscopy, imaging, and photocontrol: Foundations for super-resolution microscopy

  1. (William E. ).
Publication Date:
Sponsoring Org.:
OSTI Identifier:
Grant/Contract Number:  
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
Reviews of Modern Physics
Additional Journal Information:
Journal Name: Reviews of Modern Physics Journal Volume: 87 Journal Issue: 4; Journal ID: ISSN 0034-6861
American Physical Society
Country of Publication:
United States

Citation Formats

Moerner, W. E. Nobel Lecture: Single-molecule spectroscopy, imaging, and photocontrol: Foundations for super-resolution microscopy. United States: N. p., 2015. Web. doi:10.1103/RevModPhys.87.1183.
Moerner, W. E. Nobel Lecture: Single-molecule spectroscopy, imaging, and photocontrol: Foundations for super-resolution microscopy. United States. doi:10.1103/RevModPhys.87.1183.
Moerner, W. E. Wed . "Nobel Lecture: Single-molecule spectroscopy, imaging, and photocontrol: Foundations for super-resolution microscopy". United States. doi:10.1103/RevModPhys.87.1183.
title = {Nobel Lecture: Single-molecule spectroscopy, imaging, and photocontrol: Foundations for super-resolution microscopy},
author = {Moerner, W. E.},
abstractNote = {},
doi = {10.1103/RevModPhys.87.1183},
journal = {Reviews of Modern Physics},
number = 4,
volume = 87,
place = {United States},
year = {2015},
month = {10}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1103/RevModPhys.87.1183

Citation Metrics:
Cited by: 22 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Multicolor Fluorescence Nanoscopy in Fixed and Living Cells by Exciting Conventional Fluorophores with a Single Wavelength
journal, October 2010

High Accuracy 3D Quantum Dot Tracking with Multifocal Plane Microscopy for the Study of Fast Intracellular Dynamics in Live Cells
journal, December 2008

Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor.
journal, June 1996

  • Ha, T.; Enderle, T.; Ogletree, D. F.
  • Proceedings of the National Academy of Sciences, Vol. 93, Issue 13
  • DOI: 10.1073/pnas.93.13.6264

The Green Fluorescent Protein
journal, June 1998

On/off blinking and switching behaviour of single molecules of green fluorescent protein
journal, July 1997

  • Dickson, Robert M.; Cubitt, Andrew B.; Tsien, Roger Y.
  • Nature, Vol. 388, Issue 6640
  • DOI: 10.1038/41048

Nanometer-localized multiple single-molecule fluorescence microscopy
journal, July 2004

  • Qu, X.; Wu, D.; Mets, L.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 31, p. 11298-11303
  • DOI: 10.1073/pnas.0402155101

An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein
journal, September 2002

  • Ando, R.; Hama, H.; Yamamoto-Hino, M.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 20, p. 12651-12656
  • DOI: 10.1073/pnas.202320599

Scanning tunneling microscopy—from birth to adolescence
journal, July 1987

Far-field fluorescence microscopy beyond the diffraction limit
journal, January 1999

  • van Oijen, A. M.; Köhler, J.; Schmidt, J.
  • Journal of the Optical Society of America A, Vol. 16, Issue 4
  • DOI: 10.1364/JOSAA.16.000909

Single Molecules Observed by Near-Field Scanning Optical Microscopy
journal, November 1993

Imaging and Time-Resolved Spectroscopy of Single Molecules at an Interface
journal, April 1996

Proposed method for molecular optical imaging
journal, January 1995

Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)
journal, August 2006

  • Rust, Michael J.; Bates, Mark; Zhuang, Xiaowei
  • Nature Methods, Vol. 3, Issue 10
  • DOI: 10.1038/nmeth929

Precise Nanometer Localization Analysis for Individual Fluorescent Probes
journal, May 2002

Detection of single fluorescent molecules
journal, November 1990

Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells
journal, December 2010

Methods of single-molecule fluorescence spectroscopy and microscopy
journal, August 2003

  • Moerner, W. E.; Fromm, David P.
  • Review of Scientific Instruments, Vol. 74, Issue 8
  • DOI: 10.1063/1.1589587

Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects
journal, January 2011

  • Lew, Matthew D.; Lee, Steven F.; Badieirostami, Majid
  • Optics Letters, Vol. 36, Issue 2
  • DOI: 10.1364/OL.36.000202

Magnetic resonance of a single molecular spin
journal, May 1993

  • Köhler, J.; Disselhorst, J. A. J. M.; Donckers, M. C. J. M.
  • Nature, Vol. 363, Issue 6426
  • DOI: 10.1038/363242a0

Ultrahigh-resolution multicolor colocalization of single fluorescent probes
journal, August 2000

  • Lacoste, T. D.; Michalet, X.; Pinaud, F.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 17, p. 9461-9466
  • DOI: 10.1073/pnas.170286097

Optical detection and spectroscopy of single molecules in a solid
journal, May 1989

Beiträge zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung
journal, December 1873

Optimal Point Spread Function Design for 3D Imaging
journal, September 2014

Single molecule polarization spectroscopy: pentacene in p-terphenyl
journal, November 1996

Tracking single molecules at work in living cells
journal, June 2014

  • Kusumi, Akihiro; Tsunoyama, Taka A.; Hirosawa, Kohichiro M.
  • Nature Chemical Biology, Vol. 10, Issue 7
  • DOI: 10.1038/nchembio.1558

Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution
journal, April 1995

  • Funatsu, Takashi; Harada, Yoshie; Tokunaga, Makio
  • Nature, Vol. 374, Issue 6522
  • DOI: 10.1038/374555a0

High-Resolution Colocalization of Single Dye Molecules by Fluorescence Lifetime Imaging Microscopy
journal, July 2002

  • Heilemann, Mike; Herten, Dirk P.; Heintzmann, Rainer
  • Analytical Chemistry, Vol. 74, Issue 14
  • DOI: 10.1021/ac025576g

Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy
journal, February 2008

Fluorescence correlation spectroscopy. II. An experimental realization
journal, January 1974

Fluorescence correlation spectroscopy. I. Conceptual basis and theory
journal, January 1974

Ultra-fast excited state dynamics in green fluorescent protein: multiple states and proton transfer.
journal, August 1996

  • Chattoraj, M.; King, B. A.; Bublitz, G. U.
  • Proceedings of the National Academy of Sciences, Vol. 93, Issue 16
  • DOI: 10.1073/pnas.93.16.8362

A spindle-like apparatus guides bacterial chromosome segregation
journal, July 2010

  • Ptacin, Jerod L.; Lee, Steven F.; Garner, Ethan C.
  • Nature Cell Biology, Vol. 12, Issue 8
  • DOI: 10.1038/ncb2083

A practical guide to single-molecule FRET
journal, May 2008

  • Roy, Rahul; Hohng, Sungchul; Ha, Taekjip
  • Nature Methods, Vol. 5, Issue 6
  • DOI: 10.1038/nmeth.1208

Atomic Force Microscope
journal, March 1986

Three-dimensional sub–100 nm resolution fluorescence microscopy of thick samples
journal, May 2008

  • Juette, Manuel F.; Gould, Travis J.; Lessard, Mark D.
  • Nature Methods, Vol. 5, Issue 6
  • DOI: 10.1038/nmeth.1211

Probing Single Biomolecules in Solution Using the Anti-Brownian Electrokinetic (ABEL) Trap
journal, March 2012

  • Wang, Quan; Goldsmith, Randall H.; Jiang, Yan
  • Accounts of Chemical Research, Vol. 45, Issue 11
  • DOI: 10.1021/ar200304t

Single-molecule high-resolution imaging with photobleaching
journal, April 2004

  • Gordon, M. P.; Ha, T.; Selvin, P. R.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 17, p. 6462-6465
  • DOI: 10.1073/pnas.0401638101

Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function
journal, February 2009

  • Pavani, S. R. P.; Thompson, M. A.; Biteen, J. S.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 9
  • DOI: 10.1073/pnas.0900245106

Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
journal, September 2006

Position measurement with a resolution and noise‐limited instrument
journal, June 1986

  • Bobroff, Norman
  • Review of Scientific Instruments, Vol. 57, Issue 6
  • DOI: 10.1063/1.1138619

Frequency modulation (FM) spectroscopy: Theory of lineshapes and signal-to-noise analysis
journal, November 1983

  • Bjorklund, G. C.; Levenson, M. D.; Lenth, W.
  • Applied Physics B Photophysics and Laser Chemistry, Vol. 32, Issue 3
  • DOI: 10.1007/BF00688820

Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy
journal, January 1994

Three-dimensional localization precision of the double-helix point spread function versus astigmatism and biplane
journal, October 2010

  • Badieirostami, Majid; Lew, Matthew D.; Thompson, Michael A.
  • Applied Physics Letters, Vol. 97, Issue 16
  • DOI: 10.1063/1.3499652

Biological and Chemical Applications of Fluorescence Correlation Spectroscopy:  A Review
journal, January 2002

  • Hess, Samuel T.; Huang, Shaohui; Heikal, Ahmed A.
  • Biochemistry, Vol. 41, Issue 3
  • DOI: 10.1021/bi0118512

Enzyme Kinetics, Past and Present
journal, December 2013

Quantitative Multicolor Subdiffraction Imaging of Bacterial Protein Ultrastructures in Three Dimensions
journal, February 2013

  • Gahlmann, Andreas; Ptacin, Jerod L.; Grover, Ginni
  • Nano Letters, Vol. 13, Issue 3
  • DOI: 10.1021/nl304071h

Simultaneous Imaging of Individual Molecules Aligned Both Parallel and Perpendicular to the Optic Axis
journal, December 1998

Actin, Spectrin, and Associated Proteins Form a Periodic Cytoskeletal Structure in Axons
journal, December 2012

Subdiffraction-Resolution Fluorescence Imaging with Conventional Fluorescent Probes
journal, August 2008

  • Heilemann, Mike; van de Linde, Sebastian; Schüttpelz, Mark
  • Angewandte Chemie International Edition, Vol. 47, Issue 33
  • DOI: 10.1002/anie.200802376

EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion
journal, October 2004

  • Wiedenmann, J.; Ivanchenko, S.; Oswald, F.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 45
  • DOI: 10.1073/pnas.0403668101

Wide-field subdiffraction imaging by accumulated binding of diffusing probes
journal, December 2006

  • Sharonov, A.; Hochstrasser, R. M.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 50
  • DOI: 10.1073/pnas.0609643104

Real-Time Single-Molecule Imaging of the Infection Pathway of an Adeno-Associated Virus
journal, November 2001

Intersystem crossing from singlet states of molecular dimers and monomers in mixed molecular crystals: picosecond stimulated photon echo experiments
journal, February 1984

Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy
journal, December 2006

Superresolution by localization of quantum dots using blinking statistics
journal, January 2005

  • Lidke, Keith A.; Rieger, Bernd; Jovin, Thomas M.
  • Optics Express, Vol. 13, Issue 18
  • DOI: 10.1364/OPEX.13.007052

Fluorescence spectroscopy and spectral diffusion of single impurity molecules in a crystal
journal, January 1991

  • Ambrose, W. P.; Moerner, W. E.
  • Nature, Vol. 349, Issue 6306
  • DOI: 10.1038/349225a0

Fluorescence correlation spectroscopy. III. Uniform translation and laminar flow
journal, February 1978

The Role of Molecular Dipole Orientation in Single-Molecule Fluorescence Microscopy and Implications for Super-Resolution Imaging
journal, December 2013

  • Backlund, Mikael P.; Lew, Matthew D.; Backer, Adam S.
  • ChemPhysChem, Vol. 15, Issue 4
  • DOI: 10.1002/cphc.201300880

Illuminating Single Molecules in Condensed Matter
journal, March 1999

Small-Molecule Labeling of Live Cell Surfaces for Three-Dimensional Super-Resolution Microscopy
journal, August 2014

  • Lee, Marissa K.; Rai, Prabin; Williams, Jarrod
  • Journal of the American Chemical Society, Vol. 136, Issue 40
  • DOI: 10.1021/ja508028h

Fluorescence Spectroscopy of Single Biomolecules
journal, March 1999

Large single-molecule fluorescence enhancements produced by a bowtie nanoantenna
journal, October 2009

  • Kinkhabwala, Anika; Yu, Zongfu; Fan, Shanhui
  • Nature Photonics, Vol. 3, Issue 11, p. 654-657
  • DOI: 10.1038/nphoton.2009.187

Shapes of Inhomogeneously Broadened Resonance Lines in Solids
journal, January 1969

Single-channel currents recorded from membrane of denervated frog muscle fibres
journal, April 1976

  • Neher, Erwin; Sakmann, Bert
  • Nature, Vol. 260, Issue 5554
  • DOI: 10.1038/260799a0