The activity of CouR, a MarR family transcriptional regulator, is modulated through a novel molecular mechanism
Abstract
CouR, a MarR-type transcriptional repressor, regulates the cou genes, encoding p-hydroxycinnamate catabolism in the soil bacterium Rhodococcus jostii RHA1. The CouR dimer bound two molecules of the catabolite p-coumaroyl–CoA (Kd = 11 ± 1 μM). The presence of p-coumaroyl–CoA, but neither p-coumarate nor CoASH, abrogated CouR's binding to its operator DNA in vitro. The crystal structures of ligand-free CouR and its p-coumaroyl–CoA-bound form showed no significant conformational differences, in contrast to other MarR regulators. The CouR–p-coumaroyl–CoA structure revealed two ligand molecules bound to the CouR dimer with their phenolic moieties occupying equivalent hydrophobic pockets in each protomer and their CoA moieties adopting non-equivalent positions to mask the regulator's predicted DNA-binding surface. More specifically, the CoA phosphates formed salt bridges with predicted DNA-binding residues Arg36 and Arg38, changing the overall charge of the DNA-binding surface. The substitution of either arginine with alanine completely abrogated the ability of CouR to bind DNA. By contrast, the R36A/R38A double variant retained a relatively high affinity for p-coumaroyl–CoA (Kd = 89 ± 6 μM). Altogether, our data point to a novel mechanism of action in which the ligand abrogates the repressor's ability to bind DNA by steric occlusion of key DNA-binding residues and charge repulsionmore »
- Authors:
-
- The Univ. of British Columbia, Vancouver, BC (Canada)
- Univ. of Toronto, Toronto, ON (Canada)
- Argonne National Lab. (ANL) and the Midwest Center for Structural Genomics, Lemont, IL (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1223714
- Alternate Identifier(s):
- OSTI ID: 1258748
- Grant/Contract Number:
- AC02-06CH11357
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nucleic Acids Research
- Additional Journal Information:
- Journal Name: Nucleic Acids Research; Journal ID: ISSN 0305-1048
- Publisher:
- Oxford University Press
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Otani, Hiroshi, Stogios, Peter J., Xu, Xiaohui, Nocek, Boguslaw, Li, Shu -Nan, Savchenko, Alexei, and Eltis, Lindsay D. The activity of CouR, a MarR family transcriptional regulator, is modulated through a novel molecular mechanism. United States: N. p., 2015.
Web. doi:10.1093/nar/gkv955.
Otani, Hiroshi, Stogios, Peter J., Xu, Xiaohui, Nocek, Boguslaw, Li, Shu -Nan, Savchenko, Alexei, & Eltis, Lindsay D. The activity of CouR, a MarR family transcriptional regulator, is modulated through a novel molecular mechanism. United States. https://doi.org/10.1093/nar/gkv955
Otani, Hiroshi, Stogios, Peter J., Xu, Xiaohui, Nocek, Boguslaw, Li, Shu -Nan, Savchenko, Alexei, and Eltis, Lindsay D. Tue .
"The activity of CouR, a MarR family transcriptional regulator, is modulated through a novel molecular mechanism". United States. https://doi.org/10.1093/nar/gkv955. https://www.osti.gov/servlets/purl/1223714.
@article{osti_1223714,
title = {The activity of CouR, a MarR family transcriptional regulator, is modulated through a novel molecular mechanism},
author = {Otani, Hiroshi and Stogios, Peter J. and Xu, Xiaohui and Nocek, Boguslaw and Li, Shu -Nan and Savchenko, Alexei and Eltis, Lindsay D.},
abstractNote = {CouR, a MarR-type transcriptional repressor, regulates the cou genes, encoding p-hydroxycinnamate catabolism in the soil bacterium Rhodococcus jostii RHA1. The CouR dimer bound two molecules of the catabolite p-coumaroyl–CoA (Kd = 11 ± 1 μM). The presence of p-coumaroyl–CoA, but neither p-coumarate nor CoASH, abrogated CouR's binding to its operator DNA in vitro. The crystal structures of ligand-free CouR and its p-coumaroyl–CoA-bound form showed no significant conformational differences, in contrast to other MarR regulators. The CouR–p-coumaroyl–CoA structure revealed two ligand molecules bound to the CouR dimer with their phenolic moieties occupying equivalent hydrophobic pockets in each protomer and their CoA moieties adopting non-equivalent positions to mask the regulator's predicted DNA-binding surface. More specifically, the CoA phosphates formed salt bridges with predicted DNA-binding residues Arg36 and Arg38, changing the overall charge of the DNA-binding surface. The substitution of either arginine with alanine completely abrogated the ability of CouR to bind DNA. By contrast, the R36A/R38A double variant retained a relatively high affinity for p-coumaroyl–CoA (Kd = 89 ± 6 μM). Altogether, our data point to a novel mechanism of action in which the ligand abrogates the repressor's ability to bind DNA by steric occlusion of key DNA-binding residues and charge repulsion of the DNA backbone.},
doi = {10.1093/nar/gkv955},
journal = {Nucleic Acids Research},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 22 00:00:00 EDT 2015},
month = {Tue Sep 22 00:00:00 EDT 2015}
}
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