The kinase LYK5 is a major chitin receptor in Arabidopsis and forms a chitin-induced complex with related kinase CERK1
Chitin is a fungal microbe-associated molecular pattern recognized in Arabidopsis by a lysin motif receptor kinase (LYK), AtCERK1. Previous research suggested that AtCERK1 is the major chitin receptor and mediates chitin-induced signaling through homodimerization and phosphorylation. However, the reported chitin binding affinity of AtCERK1 is quite low, suggesting another receptor with high chitin binding affinity might be present. Here, we propose that AtLYK5 is the primary chitin receptor in Arabidopsis. Mutations in AtLYK5 resulted in a significant reduction in chitin response. AtLYK5 shares overlapping function with AtLYK4 and, therefore, Atlyk4/Atlyk5-2 double mutants show a complete loss of chitin response. AtLYK5 interacts with AtCERK1 in a chitin-dependent manner. Chitin binding to AtLYK5 is indispensable for chitin-induced AtCERK1 phosphorylation. AtLYK5 binds chitin at a much higher affinity than AtCERK1. The data suggest that AtLYK5 is the primary receptor for chitin, forming a chitin inducible complex with AtCERK1 to induce plant immunity.
- Univ. of Missouri, Columbia, MO (United States). Div. of Plant Sciences and Dept. of Biochemistry.
- Argonne National Lab., IL (United States). Midwest Center for Structural Genomics.
- Publication Date:
- OSTI Identifier:
- Grant/Contract Number:
- AC02-06CH11357; FG02-08ER15309
- Accepted Manuscript
- Journal Name:
- Additional Journal Information:
- Journal Volume: 3; Journal ID: ISSN 2050-084X
- eLife Sciences Publications, Ltd.
- Research Org:
- Argonne National Laboratory (ANL), Argonne, IL (United States); Univ. of Missouri, Columbia, MO (United States)
- Sponsoring Org:
- USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
- Country of Publication:
- United States
- 59 BASIC BIOLOGICAL SCIENCES