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Title: Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions

Abstract

Aims: To better understand the parameters that govern spore dissemination after lung exposure using in vitro cell systems. Methods and Results: We evaluated the kinetics of uptake, germination and proliferation of B. anthracis Sterne spores in association with human primary lung epithelial cells, Calu-3, and A549 cell lines. We also analyzed the influence of various cell culture media formulations related to spore germination. Conclusions: We found negligible spore uptake by epithelial cells, but germination and proliferation of spores in the extracellular environment was evident, and was appreciably higher in A549 and Calu-3 cultures than in primary epithelial cells. Additionally, our results revealed spores in association with primary cells submerged in cell culture media germinated 1 h

Authors:
 [1];  [1];  [1];  [1]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Chemical and Biological Signature Sciences Group
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1222891
Report Number(s):
PNNL-SA-109060
400904120
Grant/Contract Number:  
AC05-76RL01830
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Applied Microbiology, 119(3):711-723
Additional Journal Information:
Journal Name: Journal of Applied Microbiology, 119(3):711-723
Country of Publication:
United States
Language:
English

Citation Formats

Powell, Joshua D., Hutchison, Janine R., Hess, Becky M., and Straub, Tim M. Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions. United States: N. p., 2015. Web. doi:10.1111/jam.12872.
Powell, Joshua D., Hutchison, Janine R., Hess, Becky M., & Straub, Tim M. Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions. United States. doi:10.1111/jam.12872.
Powell, Joshua D., Hutchison, Janine R., Hess, Becky M., and Straub, Tim M. Thu . "Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions". United States. doi:10.1111/jam.12872. https://www.osti.gov/servlets/purl/1222891.
@article{osti_1222891,
title = {Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions},
author = {Powell, Joshua D. and Hutchison, Janine R. and Hess, Becky M. and Straub, Tim M.},
abstractNote = {Aims: To better understand the parameters that govern spore dissemination after lung exposure using in vitro cell systems. Methods and Results: We evaluated the kinetics of uptake, germination and proliferation of B. anthracis Sterne spores in association with human primary lung epithelial cells, Calu-3, and A549 cell lines. We also analyzed the influence of various cell culture media formulations related to spore germination. Conclusions: We found negligible spore uptake by epithelial cells, but germination and proliferation of spores in the extracellular environment was evident, and was appreciably higher in A549 and Calu-3 cultures than in primary epithelial cells. Additionally, our results revealed spores in association with primary cells submerged in cell culture media germinated 1 h},
doi = {10.1111/jam.12872},
journal = {Journal of Applied Microbiology, 119(3):711-723},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {7}
}

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