Human non-neutralizing HIV-1 envelope monoclonal antibodies limit the number of founder viruses during SHIV mucosal infection in rhesus macaques
Abstract
HIV-1 mucosal transmission begins with virus or virus-infected cells moving through mucus across mucosal epithelium to infect CD4⁺ T cells. Although broadly neutralizing antibodies (bnAbs) are the type of HIV-1 antibodies that are most likely protective, they are not induced with current vaccine candidates. In contrast, antibodies that do not neutralize primary HIV-1 strains in the TZM-bl infection assay are readily induced by current vaccine candidates and have also been implicated as secondary correlates of decreased HIV-1 risk in the RV144 vaccine efficacy trial. Here, we have studied the capacity of anti-Env monoclonal antibodies (mAbs) against either the immunodominant region of gp41 (7B2 IgG1), the first constant region of gp120 (A32 IgG1), or the third variable loop (V3) of gp120 (CH22 IgG1) to modulate in vivo rectal mucosal transmission of a high-dose simian-human immunodeficiency virus (SHIV-BaL) in rhesus macaques. 7B2 IgG1 or A32 IgG1, each containing mutations to enhance Fc function, was administered passively to rhesus macaques but afforded no protection against productive clinical infection while the positive control antibody CH22 IgG1 prevented infection in 4 of 6 animals. Enumeration of transmitted/founder (T/F) viruses revealed that passive infusion of each of the three antibodies significantly reduced the number of T/Fmore »
- Authors:
-
more »
- Harvard Medical School, Boston, MA (United States)
- Duke School of Medicine, Durham, NC (United States)
- Univ. of Pennsylvania, Philadelphia, PA (United States)
- Imperial College London, London (United Kingdom)
- Tulane Univ. School of Medicine, New Orleans, LA (United States)
- Univ. of California, Irvine, CA (United States)
- Mahidol Univ., Bangkok (Thailand)
- Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok (Thailand)
- Ministry of Public Health, Nonthaburi (Thailand)
- Walter Reed Army Institute of Research, Silver Springs, MD (United States)
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- INSERM Univ. of Strasbourg, Alsace (France)
- Vaccine Research Center, Bethesda, MD (United States)
- Publication Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1222591
- Grant/Contract Number:
- AC52-06NA25396
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS Pathogens
- Additional Journal Information:
- Journal Volume: 11; Journal Issue: 8; Journal ID: ISSN 1553-7374
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; antibodies; HIV-1; virions; macaque; rhesus monkeys; macrophages; enzyme-linked immunoassays; T cells
Citation Formats
Santra, Sampa, Tomaras, Georgia D., Warrier, Ranjit, Nicely, Nathan I., Liao, Hua -Xin, Pollara, Justin, Liu, Pinghuang, Alam, S. Munir, Zhang, Ruijun, Cocklin, Sarah L., Shen, Xiaoying, Duffy, Ryan, Xia, Shi -Mao, Schutte, Robert J., Pemble IV, Charles W., Dennison, S. Moses, Li, Hui, Chao, Andrew, Vidnovic, Kora, Evans, Abbey, Klein, Katja, Kumar, Amit, Robinson, James, Landucci, Gary, Forthal, Donald N., Montefiori, David C., Kaewkungwal, Jaranit, Nitayaphan, Sorachai, Pitisuttithum, Punnee, Rerks-Ngarm, Supachai, Robb, Merlin L., Michael, Nelson L., Kim, Jerome H., Soderberg, Kelly A., Giorgi, Elena E., Blair, Lily, Korber, Bette T., Moog, Christiane, Shattock, Robin J., Letvin, Norman L., Schmitz, Joern E., Moody, M. A., Gao, Feng, Ferrari, Guido, Shaw, George M., Haynes, Barton F., and Douek, Daniel C. Human non-neutralizing HIV-1 envelope monoclonal antibodies limit the number of founder viruses during SHIV mucosal infection in rhesus macaques. United States: N. p., 2015.
Web. doi:10.1371/journal.ppat.1005042.
Santra, Sampa, Tomaras, Georgia D., Warrier, Ranjit, Nicely, Nathan I., Liao, Hua -Xin, Pollara, Justin, Liu, Pinghuang, Alam, S. Munir, Zhang, Ruijun, Cocklin, Sarah L., Shen, Xiaoying, Duffy, Ryan, Xia, Shi -Mao, Schutte, Robert J., Pemble IV, Charles W., Dennison, S. Moses, Li, Hui, Chao, Andrew, Vidnovic, Kora, Evans, Abbey, Klein, Katja, Kumar, Amit, Robinson, James, Landucci, Gary, Forthal, Donald N., Montefiori, David C., Kaewkungwal, Jaranit, Nitayaphan, Sorachai, Pitisuttithum, Punnee, Rerks-Ngarm, Supachai, Robb, Merlin L., Michael, Nelson L., Kim, Jerome H., Soderberg, Kelly A., Giorgi, Elena E., Blair, Lily, Korber, Bette T., Moog, Christiane, Shattock, Robin J., Letvin, Norman L., Schmitz, Joern E., Moody, M. A., Gao, Feng, Ferrari, Guido, Shaw, George M., Haynes, Barton F., & Douek, Daniel C. Human non-neutralizing HIV-1 envelope monoclonal antibodies limit the number of founder viruses during SHIV mucosal infection in rhesus macaques. United States. https://doi.org/10.1371/journal.ppat.1005042
Santra, Sampa, Tomaras, Georgia D., Warrier, Ranjit, Nicely, Nathan I., Liao, Hua -Xin, Pollara, Justin, Liu, Pinghuang, Alam, S. Munir, Zhang, Ruijun, Cocklin, Sarah L., Shen, Xiaoying, Duffy, Ryan, Xia, Shi -Mao, Schutte, Robert J., Pemble IV, Charles W., Dennison, S. Moses, Li, Hui, Chao, Andrew, Vidnovic, Kora, Evans, Abbey, Klein, Katja, Kumar, Amit, Robinson, James, Landucci, Gary, Forthal, Donald N., Montefiori, David C., Kaewkungwal, Jaranit, Nitayaphan, Sorachai, Pitisuttithum, Punnee, Rerks-Ngarm, Supachai, Robb, Merlin L., Michael, Nelson L., Kim, Jerome H., Soderberg, Kelly A., Giorgi, Elena E., Blair, Lily, Korber, Bette T., Moog, Christiane, Shattock, Robin J., Letvin, Norman L., Schmitz, Joern E., Moody, M. A., Gao, Feng, Ferrari, Guido, Shaw, George M., Haynes, Barton F., and Douek, Daniel C. Mon .
"Human non-neutralizing HIV-1 envelope monoclonal antibodies limit the number of founder viruses during SHIV mucosal infection in rhesus macaques". United States. https://doi.org/10.1371/journal.ppat.1005042. https://www.osti.gov/servlets/purl/1222591.
@article{osti_1222591,
title = {Human non-neutralizing HIV-1 envelope monoclonal antibodies limit the number of founder viruses during SHIV mucosal infection in rhesus macaques},
author = {Santra, Sampa and Tomaras, Georgia D. and Warrier, Ranjit and Nicely, Nathan I. and Liao, Hua -Xin and Pollara, Justin and Liu, Pinghuang and Alam, S. Munir and Zhang, Ruijun and Cocklin, Sarah L. and Shen, Xiaoying and Duffy, Ryan and Xia, Shi -Mao and Schutte, Robert J. and Pemble IV, Charles W. and Dennison, S. Moses and Li, Hui and Chao, Andrew and Vidnovic, Kora and Evans, Abbey and Klein, Katja and Kumar, Amit and Robinson, James and Landucci, Gary and Forthal, Donald N. and Montefiori, David C. and Kaewkungwal, Jaranit and Nitayaphan, Sorachai and Pitisuttithum, Punnee and Rerks-Ngarm, Supachai and Robb, Merlin L. and Michael, Nelson L. and Kim, Jerome H. and Soderberg, Kelly A. and Giorgi, Elena E. and Blair, Lily and Korber, Bette T. and Moog, Christiane and Shattock, Robin J. and Letvin, Norman L. and Schmitz, Joern E. and Moody, M. A. and Gao, Feng and Ferrari, Guido and Shaw, George M. and Haynes, Barton F. and Douek, Daniel C.},
abstractNote = {HIV-1 mucosal transmission begins with virus or virus-infected cells moving through mucus across mucosal epithelium to infect CD4⁺ T cells. Although broadly neutralizing antibodies (bnAbs) are the type of HIV-1 antibodies that are most likely protective, they are not induced with current vaccine candidates. In contrast, antibodies that do not neutralize primary HIV-1 strains in the TZM-bl infection assay are readily induced by current vaccine candidates and have also been implicated as secondary correlates of decreased HIV-1 risk in the RV144 vaccine efficacy trial. Here, we have studied the capacity of anti-Env monoclonal antibodies (mAbs) against either the immunodominant region of gp41 (7B2 IgG1), the first constant region of gp120 (A32 IgG1), or the third variable loop (V3) of gp120 (CH22 IgG1) to modulate in vivo rectal mucosal transmission of a high-dose simian-human immunodeficiency virus (SHIV-BaL) in rhesus macaques. 7B2 IgG1 or A32 IgG1, each containing mutations to enhance Fc function, was administered passively to rhesus macaques but afforded no protection against productive clinical infection while the positive control antibody CH22 IgG1 prevented infection in 4 of 6 animals. Enumeration of transmitted/founder (T/F) viruses revealed that passive infusion of each of the three antibodies significantly reduced the number of T/F genomes. Some antibodies that bind HIV-1 Env but fail to neutralize virus in traditional neutralization assays may limit the number of T/F viruses involved in transmission without leading to enhancement of viral infection. For one of these mAbs, gp41 mAb 7B2, we provide the first co-crystal structure in complex with a common cyclical loop motif demonstrated to be critical for infection by other retroviruses.},
doi = {10.1371/journal.ppat.1005042},
journal = {PLoS Pathogens},
number = 8,
volume = 11,
place = {United States},
year = {Mon Aug 03 00:00:00 EDT 2015},
month = {Mon Aug 03 00:00:00 EDT 2015}
}
Web of Science
Works referenced in this record:
Highly potent HIV-specific antibody neutralization in vitro translates into effective protection against mucosal SHIV challenge in vivo
journal, October 2012
- Moldt, B.; Rakasz, E. G.; Schultz, N.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 46
A Substantial Transmission Bottleneck among Newly and Recently HIV‐1–Infected Injection Drug Users in St Petersburg, Russia
journal, June 2010
- Masharsky, Alexey E.; Dukhovlinova, Elena N.; Verevochkin, Sergei V.
- The Journal of Infectious Diseases, Vol. 201, Issue 11
Efficacy Trial of a DNA/rAd5 HIV-1 Preventive Vaccine
journal, November 2013
- Hammer, Scott M.; Sobieszczyk, Magdalena E.; Janes, Holly
- New England Journal of Medicine, Vol. 369, Issue 22
Structure of a human monoclonal antibody Fab fragment against gp41 of human immunodeficiency virus type 1.
journal, August 1992
- He, X. M.; Ruker, F.; Casale, E.
- Proceedings of the National Academy of Sciences, Vol. 89, Issue 15
Specificity and 6-Month Durability of Immune Responses Induced by DNA and Recombinant Modified Vaccinia Ankara Vaccines Expressing HIV-1 Virus-Like Particles
journal, January 2014
- Goepfert, Paul A.; Elizaga, Marnie L.; Seaton, Kelly
- The Journal of Infectious Diseases, Vol. 210, Issue 1
Conserved and Exposed Epitopes on Intact, Native, Primary Human Immunodeficiency Virus Type 1 Virions of Group M
journal, August 2000
- Nyambi, P. N.; Mbah, H. A.; Burda, S.
- Journal of Virology, Vol. 74, Issue 15
Nonneutralizing Antibodies Are Able To Inhibit Human Immunodeficiency Virus Type 1 Replication in Macrophages and Immature Dendritic Cells
journal, May 2006
- Holl, V.; Peressin, M.; Decoville, T.
- Journal of Virology, Vol. 80, Issue 12
Plasma IgG to Linear Epitopes in the V2 and V3 Regions of HIV-1 gp120 Correlate with a Reduced Risk of Infection in the RV144 Vaccine Efficacy Trial
journal, September 2013
- Gottardo, Raphael; Bailer, Robert T.; Korber, Bette T.
- PLoS ONE, Vol. 8, Issue 9
Structural characterization of a fusion glycoprotein from a retrovirus that undergoes a hybrid 2-step entry mechanism
journal, December 2013
- Aydin, Halil; Smrke, Brianna M.; Lee, Jeffrey E.
- The FASEB Journal, Vol. 27, Issue 12
Immune-Correlates Analysis of an HIV-1 Vaccine Efficacy Trial
journal, April 2012
- Haynes, Barton F.; Gilbert, Peter B.; McElrath, M. Juliana
- New England Journal of Medicine, Vol. 366, Issue 14
Improving Defences at the Portal of HIV Entry: Mucosal and Innate Immunity
journal, April 2008
- Shattock, Robin J.; Haynes, Barton F.; Pulendran, Bali
- PLoS Medicine, Vol. 5, Issue 4
Recombinant soluble human Fcγ receptor I with picomolar affinity for immunoglobulin G
journal, December 2005
- Paetz, Antje; Sack, Markus; Thepen, Theo
- Biochemical and Biophysical Research Communications, Vol. 338, Issue 4
Functional, Non-Clonal IgMa-Restricted B Cell Receptor Interactions with the HIV-1 Envelope gp41 Membrane Proximal External Region
journal, October 2009
- Verkoczy, Laurent; Moody, M. Anthony; Holl, T. Matt
- PLoS ONE, Vol. 4, Issue 10
The role of dynamic conformational ensembles in biomolecular recognition
journal, October 2009
- Boehr, David D.; Nussinov, Ruth; Wright, Peter E.
- Nature Chemical Biology, Vol. 5, Issue 11
N-terminal substitutions in HIV-1 gp41 reduce the expression of non-trimeric envelope glycoproteins on the virus
journal, March 2008
- Dey, Antu K.; David, Kathryn B.; Ray, Neelanjana
- Virology, Vol. 372, Issue 1
Structural studies of neuropilin/antibody complexes provide insights into semaphorin and VEGF binding
journal, November 2007
- Appleton, Brent A.; Wu, Ping; Maloney, Janice
- The EMBO Journal, Vol. 26, Issue 23
Diverse specificity and effector function among human antibodies to HIV-1 envelope glycoprotein epitopes exposed by CD4 binding
journal, December 2012
- Guan, Y.; Pazgier, M.; Sajadi, M. M.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 1
A Recombinant Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Complex Stabilized by an Intermolecular Disulfide Bond between the gp120 and gp41 Subunits Is an Antigenic Mimic of the Trimeric Virion-Associated Structure
journal, January 2000
- Binley, J. M.; Sanders, R. W.; Clas, B.
- Journal of Virology, Vol. 74, Issue 2
Broadly Neutralizing Monoclonal Antibodies 2F5 and 4E10 Directed against the Human Immunodeficiency Virus Type 1 gp41 Membrane-Proximal External Region Protect against Mucosal Challenge by Simian-Human Immunodeficiency Virus SHIVBa-L
journal, November 2009
- Hessell, A. J.; Rakasz, E. G.; Tehrani, D. M.
- Journal of Virology, Vol. 84, Issue 3
Enhanced neonatal Fc receptor function improves protection against primate SHIV infection
journal, August 2014
- Ko, Sung-Youl; Pegu, Amarendra; Rudicell, Rebecca S.
- Nature, Vol. 514, Issue 7524
Wide Variation in the Multiplicity of HIV-1 Infection among Injection Drug Users
journal, April 2010
- Bar, K. J.; Li, H.; Chamberland, A.
- Journal of Virology, Vol. 84, Issue 12
High-throughput isolation of immunoglobulin genes from single human B cells and expression as monoclonal antibodies
journal, June 2009
- Liao, Hua-Xin; Levesque, Marc C.; Nagel, Ashleigh
- Journal of Virological Methods, Vol. 158, Issue 1-2
Studies of the "Chain Reversal Regions" of the Avian Sarcoma/Leukosis Virus (ASLV) and Ebolavirus Fusion Proteins: Analogous Residues Are Important, and a His Residue Unique to EnvA Affects the pH Dependence of ASLV Entry
journal, March 2010
- Delos, S. E.; La, B.; Gilmartin, A.
- Journal of Virology, Vol. 84, Issue 11
Vaccine-induced plasma IgA specific for the C1 region of the HIV-1 envelope blocks binding and effector function of IgG
journal, May 2013
- Tomaras, G. D.; Ferrari, G.; Shen, X.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 22
Neutralizing as well as non-neutralizing polyclonal immunoglobulin (Ig)G from infected patients capture HIV-1 via antibodies directed against the principal immunodominant domain of gp41
journal, March 2005
- Burrer, Renaud; Haessig-Einius, Sandrine; Aubertin, Anne-Marie
- Virology, Vol. 333, Issue 1
The Structure of an Antitumor CH2-domain-deleted Humanized Antibody
journal, May 2005
- Larson, Steven B.; Day, John S.; Glaser, Scott
- Journal of Molecular Biology, Vol. 348, Issue 5
Identification and characterization of transmitted and early founder virus envelopes in primary HIV-1 infection
journal, May 2008
- Keele, B. F.; Giorgi, E. E.; Salazar-Gonzalez, J. F.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 21
GP41-Specific Antibody Blocks Cell-Free HIV Type 1 Transcytosis through Human Rectal Mucosa and Model Colonic Epithelium
journal, March 2010
- Shen, Ruizhong; Drelichman, Ernesto R.; Bimczok, Diane
- The Journal of Immunology, Vol. 184, Issue 7
Crystal Structure of the Ebola Virus Membrane Fusion Subunit, GP2, from the Envelope Glycoprotein Ectodomain
journal, November 1998
- Weissenhorn, Winfried; Carfí, Andrea; Lee, Kon-Ho
- Molecular Cell, Vol. 2, Issue 5
Quantitating the Multiplicity of Infection with Human Immunodeficiency Virus Type 1 Subtype C Reveals a Non-Poisson Distribution of Transmitted Variants
journal, February 2009
- Abrahams, M. -R.; Anderson, J. A.; Giorgi, E. E.
- Journal of Virology, Vol. 83, Issue 8
Vaccination with ALVAC and AIDSVAX to Prevent HIV-1 Infection in Thailand
journal, December 2009
- Rerks-Ngarm, Supachai; Pitisuttithum, Punnee; Nitayaphan, Sorachai
- New England Journal of Medicine, Vol. 361, Issue 23
Interaction between a Fab fragment against gp41 of human immunodeficiency virus 1 and its peptide epitope: characterization using a peptide epitope library and molecular modeling
journal, January 1995
- Stigler, Rolf-Dietrich; Rüker, Florian; Katinger, Dietmar
- "Protein Engineering, Design and Selection", Vol. 8, Issue 5
Three-dimensional solution structure of the 44kDa ectodomain of SIV gp41
journal, August 1998
- Caffrey, M.
- The EMBO Journal, Vol. 17, Issue 16
High Multiplicity Infection by HIV-1 in Men Who Have Sex with Men
journal, May 2010
- Li, Hui; Bar, Katharine J.; Wang, Shuyi
- PLoS Pathogens, Vol. 6, Issue 5
Dynamic Antibody Specificities and Virion Concentrations in Circulating Immune Complexes in Acute to Chronic HIV-1 Infection
journal, August 2011
- Liu, P.; Overman, R. G.; Yates, N. L.
- Journal of Virology, Vol. 85, Issue 21
Nature of Nonfunctional Envelope Proteins on the Surface of Human Immunodeficiency Virus Type 1
journal, February 2006
- Moore, P. L.; Crooks, E. T.; Porter, L.
- Journal of Virology, Vol. 80, Issue 5
Extensive complement-dependent enhancement of HIV-1 by autologous non-neutralising antibodies at early stages of infection
journal, January 2011
- Willey, Suzanne; Aasa-Chapman, Marlén MI; O'Farrell, Stephen
- Retrovirology, Vol. 8, Issue 1
Live Simian Immunodeficiency Virus Vaccine Correlate of Protection: Local Antibody Production and Concentration on the Path of Virus Entry
journal, August 2014
- Li, Qingsheng; Zeng, Ming; Duan, Lijie
- The Journal of Immunology, Vol. 193, Issue 6
The Role of Antibody Polyspecificity and Lipid Reactivity in Binding of Broadly Neutralizing Anti-HIV-1 Envelope Human Monoclonal Antibodies 2F5 and 4E10 to Glycoprotein 41 Membrane Proximal Envelope Epitopes
journal, March 2007
- Alam, S. Munir; McAdams, Mildred; Boren, David
- The Journal of Immunology, Vol. 178, Issue 7
Cardiolipin Polyspecific Autoreactivity in Two Broadly Neutralizing HIV-1 Antibodies
journal, June 2005
- Haynes, B. F.
- Science, Vol. 308, Issue 5730
PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
Initial B-Cell Responses to Transmitted Human Immunodeficiency Virus Type 1: Virion-Binding Immunoglobulin M (IgM) and IgG Antibodies Followed by Plasma Anti-gp41 Antibodies with Ineffective Control of Initial Viremia
journal, October 2008
- Tomaras, G. D.; Yates, N. L.; Liu, P.
- Journal of Virology, Vol. 82, Issue 24, p. 12449-12463
Optimization of antibody binding to Fc RIIa enhances macrophage phagocytosis of tumor cells
journal, August 2008
- Richards, J. O.; Karki, S.; Lazar, G. A.
- Molecular Cancer Therapeutics, Vol. 7, Issue 8
Three-dimensional structure of a complex of antibody with influenza virus neuraminidase
journal, March 1987
- Colman, P. M.; Laver, W. G.; Varghese, J. N.
- Nature, Vol. 326, Issue 6111
Fc receptor but not complement binding is important in antibody protection against HIV
journal, September 2007
- Hessell, Ann J.; Hangartner, Lars; Hunter, Meredith
- Nature, Vol. 449, Issue 7158
Estimating time since infection in early homogeneous HIV-1 samples using a poisson model
journal, October 2010
- Giorgi, Elena E.; Funkhouser, Bob; Athreya, Gayathri
- BMC Bioinformatics, Vol. 11, Issue 1
Human Immunodeficiency Virus Type 1 Spinoculation Enhances Infection through Virus Binding
journal, November 2000
- O'Doherty, U.; Swiggard, W. J.; Malim, M. H.
- Journal of Virology, Vol. 74, Issue 21
Replication competent molecular clones of HIV-1 expressing Renilla luciferase facilitate the analysis of antibody inhibition in PBMC
journal, December 2010
- Edmonds, Tara G.; Ding, Haitao; Yuan, Xing
- Virology, Vol. 408, Issue 1
Modeling sequence evolution in acute HIV-1 infection
journal, November 2009
- Lee, Ha Youn; Giorgi, Elena E.; Keele, Brandon F.
- Journal of Theoretical Biology, Vol. 261, Issue 2
The HIV-1 gp120 CD4-Bound Conformation Is Preferentially Targeted by Antibody-Dependent Cellular Cytotoxicity-Mediating Antibodies in Sera from HIV-1-Infected Individuals
journal, October 2014
- Veillette, Maxime; Coutu, Mathieu; Richard, Jonathan
- Journal of Virology, Vol. 89, Issue 1
Neutralizing Antibodies and Control of HIV: Moves and Countermoves
journal, December 2011
- Hessell, Ann J.; Haigwood, Nancy L.
- Current HIV/AIDS Reports, Vol. 9, Issue 1
Initial antibodies binding to HIV-1 gp41 in acutely infected subjects are polyreactive and highly mutated
journal, October 2011
- Liao, Hua-Xin; Chen, Xi; Munshaw, Supriya
- The Journal of Experimental Medicine, Vol. 208, Issue 11
Human Antibodies that Neutralize HIV-1: Identification, Structures, and B Cell Ontogenies
journal, September 2012
- Kwong, Peter D.; Mascola, John R.
- Immunity, Vol. 37, Issue 3
Anti-phospholipid human monoclonal antibodies inhibit CCR5-tropic HIV-1 and induce β-chemokines
journal, April 2010
- Moody, M. Anthony; Liao, Hua-Xin; Alam, S. Munir
- The Journal of Experimental Medicine, Vol. 207, Issue 4
Heterogeneity of Envelope Molecules Expressed on Primary Human Immunodeficiency Virus Type 1 Particles as Probed by the Binding of Neutralizing and Nonneutralizing Antibodies
journal, January 2003
- Poignard, P.; Moulard, M.; Golez, E.
- Journal of Virology, Vol. 77, Issue 1
Structural Definition of an Antibody-Dependent Cellular Cytotoxicity Response Implicated in Reduced Risk for HIV-1 Infection
journal, August 2014
- Acharya, P.; Tolbert, W. D.; Gohain, N.
- Journal of Virology, Vol. 88, Issue 21
[20] Processing of X-ray diffraction data collected in oscillation mode
book, January 1997
- Otwinowski, Zbyszek; Minor, Wladek
- Macromolecular Crystallography Part A, 307-326
Role of complement and Fc receptors in the pathogenesis of HIV-1 infection
journal, January 1997
- Montefiori, David C.
- Springer Seminars in Immunopathology, Vol. 18, Issue 3
Nonneutralizing Antibodies to the CD4-Binding Site on the gp120 Subunit of Human Immunodeficiency Virus Type 1 Do Not Interfere with the Activity of a Neutralizing Antibody against the Same Site
journal, January 2003
- Herrera, C.; Spenlehauer, C.; Fung, M. S.
- Journal of Virology, Vol. 77, Issue 2
Functional Analysis of the Disulfide-Bonded Loop/Chain Reversal Region of Human Immunodeficiency Virus Type 1 gp41 Reveals a Critical Role in gp120-gp41 Association
journal, July 2001
- Maerz, A. L.; Drummer, H. E.; Wilson, K. A.
- Journal of Virology, Vol. 75, Issue 14
In Vivo Efficacy of Anti-Glycoprotein 41, But Not Anti-Glycoprotein 120, Immunotoxins in a Mouse Model of HIV Infection
journal, February 2003
- Pincus, Seth H.; Fang, Hua; Wilkinson, Royce A.
- The Journal of Immunology, Vol. 170, Issue 4
Induction of Immunity to Human Immunodeficiency Virus Type-1 by Vaccination
journal, October 2010
- McElrath, M. Juliana; Haynes, Barton F.
- Immunity, Vol. 33, Issue 4
Structure and immune recognition of trimeric pre-fusion HIV-1 Env
journal, October 2014
- Pancera, Marie; Zhou, Tongqing; Druz, Aliaksandr
- Nature, Vol. 514, Issue 7523
The Human Low Affinity Fcγ Receptors IIa, IIb, and III Bind IgG with Fast Kinetics and Distinct Thermodynamic Properties
journal, September 2001
- Maenaka, Katsumi; van der Merwe, P. Anton; Stuart, David I.
- Journal of Biological Chemistry, Vol. 276, Issue 48
Polyclonal B Cell Responses to Conserved Neutralization Epitopes in a Subset of HIV-1-Infected Individuals
journal, August 2011
- Tomaras, G. D.; Binley, J. M.; Gray, E. S.
- Journal of Virology, Vol. 85, Issue 21
Model for the structure of the HIV gp41 ectodomain: insight into the intermolecular interactions of the gp41 loop
journal, May 2001
- Caffrey, Michael
- Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Vol. 1536, Issue 2-3
Prolonged exposure of the HIV-1 gp41 membrane proximal region with L669S substitution
journal, March 2010
- Shen, X.; Dennison, S. M.; Liu, P.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 13
Herpes simplex virus 2 serostatus and viral loads of HIV-1 in blood and semen as risk factors for HIV transmission among men who have sex with men
journal, January 2008
- Butler, David M.; Smith, Davey M.; Cachay, Edward R.
- AIDS, Vol. 22, Issue 13
Crystal structure of human T cell leukemia virus type 1 gp21 ectodomain crystallized as a maltose-binding protein chimera reveals structural evolution of retroviral transmembrane proteins
journal, April 1999
- Kobe, B.; Center, R. J.; Kemp, B. E.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 8
Advancing Toward HIV-1 Vaccine Efficacy through the Intersections of Immune Correlates
journal, December 2013
- Tomaras, Georgia; Haynes, Barton
- Vaccines, Vol. 2, Issue 1
Inflammatory Genital Infections Mitigate a Severe Genetic Bottleneck in Heterosexual Transmission of Subtype A and C HIV-1
journal, January 2009
- Haaland, Richard E.; Hawkins, Paulina A.; Salazar-Gonzalez, Jesus
- PLoS Pathogens, Vol. 5, Issue 1
Iterative model building, structure refinement and density modification with the PHENIX AutoBuild wizard
journal, December 2007
- Terwilliger, Thomas C.; Grosse-Kunstleve, Ralf W.; Afonine, Pavel V.
- Acta Crystallographica Section D Biological Crystallography, Vol. 64, Issue 1
HIV-1 Vaccine-Induced C1 and V2 Env-Specific Antibodies Synergize for Increased Antiviral Activities
journal, May 2014
- Pollara, Justin; Bonsignori, Mattia; Moody, M. Anthony
- Journal of Virology, Vol. 88, Issue 14
Characterization of a Simian Human Immunodeficiency Virus Encoding the Envelope Gene from the CCR5-Tropic HIV-1 Ba-L
journal, January 2003
- Pal, Ranajit; Taylor, Brian; Foulke, J. Scott
- JAIDS Journal of Acquired Immune Deficiency Syndromes, Vol. 33, Issue 3
HIV-1 neutralizing antibodies: understanding nature's pathways
journal, June 2013
- Mascola, John R.; Haynes, Barton F.
- Immunological Reviews, Vol. 254, Issue 1
Structural and Immunological Characterisation of Heteroclitic Peptide Analogues Corresponding to the 600–612 Region of the HIV Envelope gp41 Glycoprotein
journal, October 2002
- Du, Angélique Phan Chan; Limal, David; Semetey, Vincent
- Journal of Molecular Biology, Vol. 323, Issue 3
Interaction with Cellular CD4 Exposes HIV-1 Envelope Epitopes Targeted by Antibody-Dependent Cell-Mediated Cytotoxicity
journal, December 2013
- Veillette, M.; Desormeaux, A.; Medjahed, H.
- Journal of Virology, Vol. 88, Issue 5
A New Clustering of Antibody CDR Loop Conformations
journal, February 2011
- North, Benjamin; Lehmann, Andreas; Dunbrack, Roland L.
- Journal of Molecular Biology, Vol. 406, Issue 2
Delineation of multiple subpopulations of natural killer cells in rhesus macaques
journal, June 2005
- Webster, Ramothea L.; Johnson, R. Paul
- Immunology, Vol. 115, Issue 2
Vaccine Induction of Antibodies against a Structurally Heterogeneous Site of Immune Pressure within HIV-1 Envelope Protein Variable Regions 1 and 2
journal, January 2013
- Liao, Hua-Xin; Bonsignori, Mattia; Alam, S. Munir
- Immunity, Vol. 38, Issue 1
Selective recognition of oligomeric HIV-1 primary isolate envelope glycoproteins by potently neutralizing ligands requires efficient precursor cleavage
journal, February 2005
- Pancera, Marie; Wyatt, Richard
- Virology, Vol. 332, Issue 1
Neutralizing IgG at the Portal of Infection Mediates Protection against Vaginal Simian/Human Immunodeficiency Virus Challenge
journal, August 2013
- Klein, K.; Veazey, R. S.; Warrier, R.
- Journal of Virology, Vol. 87, Issue 21
Strategies for eliciting HIV-1 inhibitory antibodies
journal, January 2010
- Tomaras, Georgia D.; Haynes, Barton F.
- Current Opinion in HIV and AIDS, Vol. 5, Issue 5
Structural evidence for induced fit as a mechanism for antibody-antigen recognition
journal, February 1992
- Rini, J.; Schulze-Gahmen, U.; Wilson, I.
- Science, Vol. 255, Issue 5047
Vaccine-Induced Env V1-V2 IgG3 Correlates with Lower HIV-1 Infection Risk and Declines Soon After Vaccination
journal, March 2014
- Yates, N. L.; Liao, H. -X.; Fong, Y.
- Science Translational Medicine, Vol. 6, Issue 228
Flexibility and molecular recognition in the immune system
journal, December 2002
- Jimenez, R.; Salazar, G.; Baldridge, K. K.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 1
Low-dose rectal inoculation of rhesus macaques by SIVsmE660 or SIVmac251 recapitulates human mucosal infection by HIV-1
journal, May 2009
- Keele, Brandon F.; Li, Hui; Learn, Gerald H.
- The Journal of Experimental Medicine, Vol. 206, Issue 5
Protective effect of vaginal application of neutralizing and nonneutralizing inhibitory antibodies against vaginal SHIV challenge in macaques
journal, April 2013
- Moog, C.; Dereuddre-Bosquet, N.; Teillaud, J-L
- Mucosal Immunology, Vol. 7, Issue 1
Retrovirus envelope domain at 1.7 Å resolution
journal, May 1996
- Fass, Deborah; Harrison, Stephen C.; Kim, Peter S.
- Nature Structural Biology, Vol. 3, Issue 5
HIV-1 gp41 envelope IgA is frequently elicited after transmission but has an initial short response half-life
journal, January 2013
- Yates, N. L.; Stacey, A. R.; Nolen, T. L.
- Mucosal Immunology, Vol. 6, Issue 4
A Nonfucosylated Variant of the anti-HIV-1 Monoclonal Antibody b12 Has Enhanced Fc RIIIa-Mediated Antiviral Activity In Vitro but Does Not Improve Protection against Mucosal SHIV Challenge in Macaques
journal, March 2012
- Moldt, B.; Shibata-Koyama, M.; Rakasz, E. G.
- Journal of Virology, Vol. 86, Issue 11
Is developing an HIV-1 vaccine possible?
journal, January 2010
- Haynes, Barton F.; Liao, Hua-Xin; Tomaras, Georgia D.
- Current Opinion in HIV and AIDS, Vol. 5, Issue 5
Limited or no protection by weakly or nonneutralizing antibodies against vaginal SHIV challenge of macaques compared with a strongly neutralizing antibody
journal, June 2011
- Burton, D. R.; Hessell, A. J.; Keele, B. F.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 27
Immunization with HIV-1 gp41 Subunit Virosomes Induces Mucosal Antibodies Protecting Nonhuman Primates against Vaginal SHIV Challenges
journal, February 2011
- Bomsel, Morgane; Tudor, Daniela; Drillet, Anne-Sophie
- Immunity, Vol. 34, Issue 2
Detecting hypermutations in viral sequences with an emphasis on G -> A hypermutation
journal, April 2000
- Rose, P. P.; Korber, B. T.
- Bioinformatics, Vol. 16, Issue 4
Magnitude and Breadth of the Neutralizing Antibody Response in the RV144 and Vax003 HIV-1 Vaccine Efficacy Trials
journal, May 2012
- Montefiori, D. C.; Karnasuta, C.; Huang, Y.
- Journal of Infectious Diseases, Vol. 206, Issue 3
Ex vivo culture of human colorectal tissue for the evaluation of candidate microbicides
journal, January 2006
- Fletcher, Patricia S.; Elliott, Julie; Grivel, Jean-Charles
- AIDS, Vol. 20, Issue 9
Topological Layers in the HIV-1 gp120 Inner Domain Regulate gp41 Interaction and CD4-Triggered Conformational Transitions
journal, March 2010
- Finzi, Andrés; Xiang, Shi-Hua; Pacheco, Beatriz
- Molecular Cell, Vol. 37, Issue 5
Structure validation by Cα geometry: ϕ,ψ and Cβ deviation
journal, January 2003
- Lovell, Simon C.; Davis, Ian W.; Arendall, W. Bryan
- Proteins: Structure, Function, and Bioinformatics, Vol. 50, Issue 3
Solvent content of protein crystals
journal, April 1968
- Matthews, B. W.
- Journal of Molecular Biology, Vol. 33, Issue 2
Human Immunodeficiency Virus Type 1 gp41 Antibodies That Mask Membrane Proximal Region Epitopes: Antibody Binding Kinetics, Induction, and Potential for Regulation in Acute Infection
journal, October 2007
- Alam, S. M.; Scearce, R. M.; Parks, R. J.
- Journal of Virology, Vol. 82, Issue 1
An HIV-1 gp120 Envelope Human Monoclonal Antibody That Recognizes a C1 Conformational Epitope Mediates Potent Antibody-Dependent Cellular Cytotoxicity (ADCC) Activity and Defines a Common ADCC Epitope in Human HIV-1 Serum
journal, May 2011
- Ferrari, G.; Pollara, J.; Kozink, D.
- Journal of Virology, Vol. 85, Issue 14
In-Solution Virus Capture Assay Helps Deconstruct Heterogeneous Antibody Recognition of Human Immunodeficiency Virus Type 1
journal, January 2010
- Leaman, D. P.; Kinkead, H.; Zwick, M. B.
- Journal of Virology, Vol. 84, Issue 7
High Resolution Mapping of the Binding Site on Human IgG1 for FcγRI, FcγRII, FcγRIII, and FcRn and Design of IgG1 Variants with Improved Binding to the FcγR
journal, November 2000
- Shields, Robert L.; Namenuk, Angela K.; Hong, Kyu
- Journal of Biological Chemistry, Vol. 276, Issue 9
Functional Implications of the Human T-Lymphotropic Virus Type 1 Transmembrane Glycoprotein Helical Hairpin Structure
journal, July 2000
- Maerz, A. L.; Center, R. J.; Kemp, B. E.
- Journal of Virology, Vol. 74, Issue 14
Broadly neutralizing human antibody that recognizes the receptor-binding pocket of influenza virus hemagglutinin
journal, August 2011
- Whittle, J. R. R.; Zhang, R.; Khurana, S.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 34
Mapping the anatomy of the immunodominant domain of the human immunodeficiency virus gp41 transmembrane protein: peptide conformation analysis using monoclonal antibodies and proton nuclear magnetic resonance spectroscopy.
journal, January 1991
- Oldstone, M. B.; Tishon, A.; Lewicki, H.
- Journal of Virology, Vol. 65, Issue 4
Effects of amino acid changes in the extracellular domain of the human immunodeficiency virus type 1 gp41 envelope glycoprotein.
journal, January 1993
- Cao, J.; Bergeron, L.; Helseth, E.
- Journal of Virology, Vol. 67, Issue 5
Conformational changes induced in the envelope glycoproteins of the human and simian immunodeficiency viruses by soluble receptor binding.
journal, January 1993
- Sattentau, Q. J.; Moore, J. P.; Vignaux, F.
- Journal of Virology, Vol. 67, Issue 12
Involvement of the V1/V2 variable loop structure in the exposure of human immunodeficiency virus type 1 gp120 epitopes induced by receptor binding.
journal, January 1995
- Wyatt, R.; Moore, J.; Accola, M.
- Journal of virology, Vol. 69, Issue 9
Protein-peptide interactions
journal, February 1995
- Stanfield, Robyn L.; Wilson, Ian A.
- Current Opinion in Structural Biology, Vol. 5, Issue 1
Infectious Virion Capture by HIV-1 gp120-Specific IgG from RV144 Vaccinees
journal, May 2013
- Liu, P.; Yates, N. L.; Shen, X.
- Journal of Virology, Vol. 87, Issue 14
Works referencing / citing this record:
HIV-1-Specific IgA Monoclonal Antibodies from an HIV-1 Vaccinee Mediate Galactosylceramide Blocking and Phagocytosis
journal, January 2018
- Wills, Saintedym; Hwang, Kwan-Ki; Liu, Pinghuang
- Journal of Virology, Vol. 92, Issue 7
IgG3 enhances neutralization potency and Fc effector function of an HIV V2-specific broadly neutralizing antibody
journal, December 2019
- Richardson, Simone I.; Lambson, Bronwen E.; Crowley, Andrew R.
- PLOS Pathogens, Vol. 15, Issue 12
Optimized Mucosal Modified Vaccinia Virus Ankara Prime/Soluble gp120 Boost HIV Vaccination Regimen Induces Antibody Responses Similar to Those of an Intramuscular Regimen
journal, May 2019
- Jones, Dorothy I.; Pollara, Justin J.; Johnson-Weaver, Brandi T.
- Journal of Virology, Vol. 93, Issue 14
Envelope residue 375 substitutions in simian–human immunodeficiency viruses enhance CD4 binding and replication in rhesus macaques
journal, May 2016
- Li, Hui; Wang, Shuyi; Kong, Rui
- Proceedings of the National Academy of Sciences, Vol. 113, Issue 24
Concurrent Exposure of Neutralizing and Non-neutralizing Epitopes on a Single HIV-1 Envelope Structure
journal, July 2019
- Ray, Krishanu; Mengistu, Meron; Orlandi, Chiara
- Frontiers in Immunology, Vol. 10