Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria
Abstract
This paper uses a mathematical model of fluorescent biological particles composed of bacteria and/or proteins (mostly as in Hill et al., 2013 [23]) to investigate the size-dependence of the total fluorescence emitted in all directions. The model applies to particles which have negligible reabsorption of fluorescence within the particle. The specific particles modeled here are composed of ovalbumin and of a generic Bacillus. The particles need not be spherical, and in some cases need not be homogeneous. However, the results calculated in this paper are for spherical homogeneous particles. Light absorbing and fluorescing molecules included in the model are amino acids, nucleic acids, and several coenzymes. Here the excitation wavelength is 266 nm. The emission range, 300 to 370 nm, encompasses the fluorescence of tryptophan. The fluorescence cross section (CF) is calculated and compared with one set of published measured values. We investigate power law (Ady) approximations to CF, where d is diameter, and A and y are parameters adjusted to fit the data, and examine how y varies with d and composition, including the fraction as water. The particle's fluorescence efficiency (QF=CF/geometric-cross-section) can be written for homogeneous particles as QabsRF, where Qabs is the absorption efficiency, and RF, themore »
- Authors:
-
- US Army Research Lab., Adelphi, MD (United States)
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Silver Spring, MD (United States)
- Publication Date:
- Research Org.:
- Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1214684
- Grant/Contract Number:
- AC04-94AL85000
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Quantitative Spectroscopy and Radiative Transfer
- Additional Journal Information:
- Journal Volume: 157; Journal Issue: C; Journal ID: ISSN 0022-4073
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; Fluorescence; Bioaerosols; Aerosol characterization; Light scattering
Citation Formats
Hill, Steven C., Williamson, Chatt C., Doughty, David C., Pan, Yong-Le, Santarpia, Joshua L., and Hill, Hanna H. Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria. United States: N. p., 2015.
Web. doi:10.1016/j.jqsrt.2015.01.011.
Hill, Steven C., Williamson, Chatt C., Doughty, David C., Pan, Yong-Le, Santarpia, Joshua L., & Hill, Hanna H. Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria. United States. https://doi.org/10.1016/j.jqsrt.2015.01.011
Hill, Steven C., Williamson, Chatt C., Doughty, David C., Pan, Yong-Le, Santarpia, Joshua L., and Hill, Hanna H. Mon .
"Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria". United States. https://doi.org/10.1016/j.jqsrt.2015.01.011. https://www.osti.gov/servlets/purl/1214684.
@article{osti_1214684,
title = {Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria},
author = {Hill, Steven C. and Williamson, Chatt C. and Doughty, David C. and Pan, Yong-Le and Santarpia, Joshua L. and Hill, Hanna H.},
abstractNote = {This paper uses a mathematical model of fluorescent biological particles composed of bacteria and/or proteins (mostly as in Hill et al., 2013 [23]) to investigate the size-dependence of the total fluorescence emitted in all directions. The model applies to particles which have negligible reabsorption of fluorescence within the particle. The specific particles modeled here are composed of ovalbumin and of a generic Bacillus. The particles need not be spherical, and in some cases need not be homogeneous. However, the results calculated in this paper are for spherical homogeneous particles. Light absorbing and fluorescing molecules included in the model are amino acids, nucleic acids, and several coenzymes. Here the excitation wavelength is 266 nm. The emission range, 300 to 370 nm, encompasses the fluorescence of tryptophan. The fluorescence cross section (CF) is calculated and compared with one set of published measured values. We investigate power law (Ady) approximations to CF, where d is diameter, and A and y are parameters adjusted to fit the data, and examine how y varies with d and composition, including the fraction as water. The particle's fluorescence efficiency (QF=CF/geometric-cross-section) can be written for homogeneous particles as QabsRF, where Qabs is the absorption efficiency, and RF, the fraction of the absorbed light emitted as fluorescence, is independent of size and shape. When QF is plotted vs. mid or mi(mr-1)d, where m=mr+imi is the complex refractive index, the plots for different fractions of water in the particle tend to overlap.},
doi = {10.1016/j.jqsrt.2015.01.011},
journal = {Journal of Quantitative Spectroscopy and Radiative Transfer},
number = C,
volume = 157,
place = {United States},
year = {Mon Feb 02 00:00:00 EST 2015},
month = {Mon Feb 02 00:00:00 EST 2015}
}
Web of Science
Works referenced in this record:
Performance Evaluation of the UVAPS in Measuring Biological Aerosols: Fluorescence Spectra from NAD(P)H Coenzymes and Riboflavin
journal, April 2004
- Agranovski, Victoria; Ristovski, Zoran D.; Ayoko, Godwin A.
- Aerosol Science and Technology, Vol. 38, Issue 4
Optical properties of ovalbumin in 0.130-2.50 ?m spectral region
journal, January 2001
- Arakawa, E. T.; Tuminello, P. S.; Khare, B. N.
- Biopolymers, Vol. 62, Issue 2
Optical properties ofErwinia herbicola bacteria at 0.190-2.50 ?m
journal, January 2003
- Arakawa, E. T.; Tuminello, P. S.; Khare, B. N.
- Biopolymers, Vol. 72, Issue 5
Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach
journal, July 2008
- Bennett, Bryson D.; Yuan, Jie; Kimball, Elizabeth H.
- Nature Protocols, Vol. 3, Issue 8
Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli
journal, June 2009
- Bennett, Bryson D.; Kimball, Elizabeth H.; Gao, Melissa
- Nature Chemical Biology, Vol. 5, Issue 8
Comparing the mechanism of water condensation and evaporation in glassy aerosol
journal, July 2012
- Bones, D. L.; Reid, J. P.; Lienhard, D. M.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 29
Violent expiratory events: on coughing and sneezing
journal, March 2014
- Bourouiba, Lydia; Dehandschoewercker, Eline; Bush, John W. M.
- Journal of Fluid Mechanics, Vol. 745
Backward enhanced emission from multiphoton processes in aerosols
journal, September 2002
- Boutou, V.; Favre, C.; Hill, S. C.
- Applied Physics B: Lasers and Optics, Vol. 75, Issue 2-3
Wet and dry density of Bacillus anthracis and other Bacillus species
journal, July 2008
- Carrera, M.; Zandomeni, R. O.; Sagripanti, J. -L.
- Journal of Applied Microbiology, Vol. 105, Issue 1
Large contribution of natural aerosols to uncertainty in indirect forcing
journal, November 2013
- Carslaw, K. S.; Lee, L. A.; Reddington, C. L.
- Nature, Vol. 503, Issue 7474
Model for Raman and fluorescent scattering by molecules embedded in small particles
journal, January 1976
- Chew, H.; McNulty, P. J.; Kerker, M.
- Physical Review A, Vol. 13, Issue 1
Ultraviolet fluorescence lidar detection of bioaerosols
conference, June 1994
- Christesen, Steven D.; Merrow, Clifton N.; DeSha, Michael S.
- SPIE's International Symposium on Optical Engineering and Photonics in Aerospace Sensing, SPIE Proceedings
Primary biological aerosol particles in the atmosphere: a review
journal, January 2012
- Després, VivianeR.; Huffman, J. Alex; Burrows, Susannah M.
- Tellus B: Chemical and Physical Meteorology, Vol. 64, Issue 1
Airborne transmission of disease in hospitals
journal, September 2009
- Eames, I.; Tang, J. W.; Li, Y.
- Journal of The Royal Society Interface, Vol. 6, Issue suppl_6
Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence
journal, April 1997
- Hairston, Peter P.; Ho, Jim; Quant, Frederick R.
- Journal of Aerosol Science, Vol. 28, Issue 3
Method for integrating the absorption cross sections of spheres over wavelength or diameter
journal, January 2003
- Hill, Steven C.
- Applied Optics, Vol. 42, Issue 21
Fluorescence of bioaerosols: mathematical model including primary fluorescing and absorbing molecules in bacteria
journal, January 2013
- Hill, Steven C.; Pan, Yong-Le; Williamson, Chatt
- Optics Express, Vol. 21, Issue 19
Fluorescence of bioaerosols: mathematical model including primary fluorescing and absorbing molecules in bacteria: errata
journal, January 2014
- Hill, Steven C.; Doughty, David C.; Pan, Yong-Le
- Optics Express, Vol. 22, Issue 19
Fluorescence from airborne microparticles: dependence on size, concentration of fluorophores, and illumination intensity
journal, January 2001
- Hill, Steven C.; Pinnick, Ronald G.; Niles, Stanley
- Applied Optics, Vol. 40, Issue 18
Size distributions and temporal variations of biological aerosol particles in the Amazon rainforest characterized by microscopy and real-time UV-APS fluorescence techniques during AMAZE-08
journal, January 2012
- Huffman, J. A.; Sinha, B.; Garland, R. M.
- Atmospheric Chemistry and Physics, Vol. 12, Issue 24
Fluorescent biological aerosol particle concentrations and size distributions measured with an Ultraviolet Aerodynamic Particle Sizer (UV-APS) in Central Europe
journal, January 2010
- Huffman, J. A.; Treutlein, B.; Pöschl, U.
- Atmospheric Chemistry and Physics, Vol. 10, Issue 7
Aerosol Generation by Modern Flush Toilets
journal, September 2013
- Johnson, David; Lynch, Robert; Marshall, Charles
- Aerosol Science and Technology, Vol. 47, Issue 9
Bioaerosol Detection with Fluorescence Spectroscopy
book, January 2014
- Jonsson, Per; Kullander, Fredrik
- Bioaerosol Detection Technologies
Buoyant Density Variation During the Cell Cycle in Microorganisms
journal, January 1987
- Kubitschek, Herbert E.
- CRC Critical Reviews in Microbiology, Vol. 14, Issue 1
A flash-lamp based device for fluorescence detection and identification of individual pollen grains
journal, March 2013
- Kiselev, Denis; Bonacina, Luigi; Wolf, Jean-Pierre
- Review of Scientific Instruments, Vol. 84, Issue 3
Effect of growth media and washing on the spectral signatures of aerosolized biological simulants
journal, January 2011
- Laflamme, Christian; Simard, Jean-Robert; Buteau, Sylvie
- Applied Optics, Vol. 50, Issue 6
The on-line detection of biological particle emissions from selected agricultural materials using the WIBS-4 (Waveband Integrated Bioaerosol Sensor) technique
journal, December 2013
- O'Connor, David J.; Healy, David A.; Sodeau, John R.
- Atmospheric Environment, Vol. 80
Backward-enhanced fluorescence from clusters of microspheres and particles of tryptophan
journal, January 2002
- Pan, Yong-Le; Hill, Steven C.; Wolf, Jean-Pierre
- Applied Optics, Vol. 41, Issue 15
Fluorescence spectra of atmospheric aerosol particles measured using one or two excitation wavelengths: Comparison of classification schemes employing different emission and scattering results
journal, January 2010
- Pan, Yong-Le; Hill, Steven C.; Pinnick, Ronald G.
- Optics Express, Vol. 18, Issue 12
Spectrally-resolved fluorescence cross sections of aerosolized biological live agents and simulants using five excitation wavelengths in a BSL-3 laboratory
journal, January 2014
- Pan, Yong-Le; Hill, Steven C.; Santarpia, Joshua L.
- Optics Express, Vol. 22, Issue 7
Single-particle laser-induced-fluorescence spectra of biological and other organic-carbon aerosols in the atmosphere: Measurements at New Haven, Connecticut, and Las Cruces, New Mexico
journal, January 2007
- Pan, Yong-Le; Pinnick, Ronald G.; Hill, Steven C.
- Journal of Geophysical Research, Vol. 112, Issue D24
Effects of ozone and relative humidity on fluorescence spectra of octapeptide bioaerosol particles
journal, January 2014
- Pan, Yong-Le; Santarpia, Joshua L.; Ratnesar-Shumate, Shanna
- Journal of Quantitative Spectroscopy and Radiative Transfer, Vol. 133
An Approximation Method to the Mie Theory for Colloidal Spheres
journal, December 1958
- Penndorf, Rudolf B.
- The Journal of Physical Chemistry, Vol. 62, Issue 12
Autofluorescence of atmospheric bioaerosols – fluorescent biomolecules and potential interferences
journal, January 2012
- Pöhlker, C.; Huffman, J. A.; Pöschl, U.
- Atmospheric Measurement Techniques, Vol. 5, Issue 1
Fluorescence properties of biochemicals in dry NaCl composite aerosol particles and in solutions
journal, May 2010
- Putkiranta, M.; Manninen, A.; Rostedt, A.
- Applied Physics B, Vol. 99, Issue 4
Cluster analysis of WIBS single-particle bioaerosol data
journal, January 2013
- Robinson, N. H.; Allan, J. D.; Huffman, J. A.
- Atmospheric Measurement Techniques, Vol. 6, Issue 2
Fluorescence spectroscopy of atmospherically relevant bacterial and fungal spores and potential interferences
journal, June 2013
- Saari, S. E.; Putkiranta, M. J.; Keskinen, J.
- Atmospheric Environment, Vol. 71
Performance of Two Fluorescence-Based Real-Time Bioaerosol Detectors: BioScout vs. UVAPS
journal, January 2014
- Saari, S.; Reponen, T.; Keskinen, J.
- Aerosol Science and Technology, Vol. 48, Issue 4
Changes in fluorescence spectra of bioaerosols exposed to ozone in a laboratory reaction chamber to simulate atmospheric aging
journal, January 2012
- Santarpia, Joshua L.; Pan, Yong-Le; Hill, Steven C.
- Optics Express, Vol. 20, Issue 28
Hydration of Proteins: Excess Partial Volumes of Water and Proteins
journal, March 2012
- Sirotkin, Vladimir A.; Komissarov, Igor A.; Khadiullina, Aigul V.
- The Journal of Physical Chemistry B, Vol. 116, Issue 13
Multiple UV wavelength excitation and fluorescence of bioaerosols
journal, January 2004
- Sivaprakasam, Vasanthi; Huston, Alan L.; Scotto, Cathy
- Optics Express, Vol. 12, Issue 19
Spectral characterization of biological aerosol particles using two-wavelength excited laser-induced fluorescence and elastic scattering measurements
journal, January 2011
- Sivaprakasam, Vasanthi; Lin, Horn-Bond; Huston, Alan L.
- Optics Express, Vol. 19, Issue 7
Long-Range Nonradiative Transfer of Electronic Excitation Energy in Proteins and Polypeptides
journal, June 1971
- Steinberg, I. Z.
- Annual Review of Biochemistry, Vol. 40, Issue 1, p. 83-114
Fluorescence from atmospheric aerosols observed with a multi-channel lidar spectrometer
journal, January 2012
- Sugimoto, Nobuo; Huang, Zhongwei; Nishizawa, Tomoaki
- Optics Express, Vol. 20, Issue 19
Atmospheric organic and bio-aerosols as cloud condensation nuclei (CCN): A review
journal, February 2006
- Sun, J.; Ariya, P.
- Atmospheric Environment, Vol. 40, Issue 5
Measurement of fluorescence spectra from atmospheric single submicron particle using laser-induced fluorescence technique
journal, April 2013
- Taketani, Fumikazu; Kanaya, Yugo; Nakamura, Takayuki
- Journal of Aerosol Science, Vol. 58
Fluorescent biological aerosol particles measured with the Waveband Integrated Bioaerosol Sensor WIBS-4: laboratory tests combined with a one year field study
journal, January 2013
- Toprak, E.; Schnaiter, M.
- Atmospheric Chemistry and Physics, Vol. 13, Issue 1
Pharmaceutical Particle Engineering via Spray Drying
journal, November 2007
- Vehring, Reinhard
- Pharmaceutical Research, Vol. 25, Issue 5
Computation of the internal field of a large spherical particle by use of the geometrical-optics approximation
journal, January 1997
- Velesco, Nadejda; Kaiser, Thomas; Schweiger, Gustav
- Applied Optics, Vol. 36, Issue 33
Geometrical optics calculation of inelastic scattering on large particles
journal, January 1999
- Velesco, Nadejda; Schweiger, Gustav
- Applied Optics, Vol. 38, Issue 6
Angle- and size-dependent characteristics of incoherent Raman and fluorescent scattering by microspheres 2 Numerical Simulation
journal, January 2002
- Veselovskii, Igor; Griaznov, Vadim; Kolgotin, Alexei
- Applied Optics, Vol. 41, Issue 27
Some Basic Characteristics of Bacterial Freezing Nuclei
journal, September 1981
- Yankofsky, S. A.; Levin, Z.; Bertold, T.
- Journal of Applied Meteorology, Vol. 20, Issue 9
Evidence of Airborne Transmission of the Severe Acute Respiratory Syndrome Virus
journal, April 2004
- Yu, Ignatius T. S.; Li, Yuguo; Wong, Tze Wai
- New England Journal of Medicine, Vol. 350, Issue 17
Works referencing / citing this record:
Systematic characterization and fluorescence threshold strategies for the wideband integrated bioaerosol sensor (WIBS) using size-resolved biological and interfering particles
journal, January 2017
- Savage, Nicole J.; Krentz, Christine E.; Könemann, Tobias
- Atmospheric Measurement Techniques, Vol. 10, Issue 11
Characterization of steady-state fluorescence properties of polystyrene latex spheres using off- and online spectroscopic methods
journal, January 2018
- Könemann, Tobias; Savage, Nicole J.; Huffman, J. Alex
- Atmospheric Measurement Techniques, Vol. 11, Issue 7
The Wavelength‐Dependent Complex Refractive Index of Hygroscopic Aerosol Particles and Other Aqueous Media: An Effective Oscillator Model
journal, September 2019
- Bain, Alison; Rafferty, Aidan; Preston, Thomas C.
- Geophysical Research Letters, Vol. 46, Issue 17-18
Real-time sensing of bioaerosols: Review and current perspectives
journal, September 2019
- Huffman, J. Alex; Perring, Anne E.; Savage, Nicole J.
- Aerosol Science and Technology, Vol. 54, Issue 5
Spectral Intensity Bioaerosol Sensor (SIBS): an instrument for spectrally resolved fluorescence detection of single particles in real time
journal, January 2019
- Könemann, Tobias; Savage, Nicole; Klimach, Thomas
- Atmospheric Measurement Techniques, Vol. 12, Issue 2
Fluorescent bioaerosol particle, molecular tracer, and fungal spore concentrations during dry and rainy periods in a semi-arid forest
journal, January 2016
- Gosselin, Marie Ila; Rathnayake, Chathurika M.; Crawford, Ian
- Atmospheric Chemistry and Physics, Vol. 16, Issue 23