Synergistic Effect of High Charge and Energy Particle Radiation and Chronological Age on Biomarkers of Oxidative Stress and Tissue Degeneration: A Ground-Based Study Using the Vertebrate Laboratory Model Organism Oryzias latipes
Abstract
High charge and energy (HZE) particles are a main hazard of the space radiation environment. Uncertainty regarding their health effects is a limiting factor in the design of human exploration-class space missions, that is, missions beyond low earth orbit. Previous work has shown that HZE exposure increases cancer risk and elicits other aging-like phenomena in animal models. Here, we investigate how a single exposure to HZE particle radiation, early in life, influences the subsequent age-dependent evolution of oxidative stress and appearance of degenerative tissue changes. Embryos of the laboratory model organism, Oryzias latipes (Japanese medaka fish), were exposed to HZE particle radiation at doses overlapping the range of anticipated human exposure. A separate cohort was exposed to reference c-radiation. Survival was monitored for 750 days, well beyond the median lifespan. The population was also sampled at intervals and liver tissue was subjected to histological and molecular analysis. HZE particle radiation dose and aging contributed synergistically to accumulation of lipid peroxidation products, which are a marker of chronic oxidative stress. This was mirrored by a decline in PPARGC1A mRNA, which encodes a transcriptional co-activator required for expression of oxidative stress defense genes and for mitochondrial maintenance. Consistent with chronic oxidative stress,more »
- Authors:
-
- Augusta Univ., Augusta, GA (United States). Dept. of Neuroscience and Regenerative Medicine; Wuhan Univ. (China). Center for Gene Diagnosis and Zhongnan Hospital
- Univ. of Alabama, Birmingham, AL (United States). Dept. of Biostatistics
- Wuhan Univ. School of Medicine (China). Dept. of Anatomy and Embryology
- Augusta Univ., Augusta, GA (United States). Dept. of Pathology
- Augusta Univ., Augusta, GA (United States). Dept. of Otolaryngology and Center for Biotechnology and Genomic Medicine
- Augusta Univ., Augusta, GA (United States). Dept. of Neuroscience and Regenerative Medicine; Emory Univ., Atlanta, GA (United States). Dept. of Radiation Oncology and Biochemistry
- Publication Date:
- Research Org.:
- Augusta Univ., Augusta, GA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; National Aeronautics and Space Administration (NASA)
- OSTI Identifier:
- 1213670
- Alternate Identifier(s):
- OSTI ID: 1458723
- Grant/Contract Number:
- SC0002343; NNX11AC30G
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 11; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; dose prediction methods; gamma rays; mitochondria; gamma radiation; oxidative stress; embryos; hematoxylin staining; DNA damage
Citation Formats
Zheng, Xuan, Zhang, Xinyan, Ding, Lingling, Lee, Jeffrey R., Weinberger, Paul M., and Dynan, William S. Synergistic Effect of High Charge and Energy Particle Radiation and Chronological Age on Biomarkers of Oxidative Stress and Tissue Degeneration: A Ground-Based Study Using the Vertebrate Laboratory Model Organism Oryzias latipes. United States: N. p., 2014.
Web. doi:10.1371/journal.pone.0111362.
Zheng, Xuan, Zhang, Xinyan, Ding, Lingling, Lee, Jeffrey R., Weinberger, Paul M., & Dynan, William S. Synergistic Effect of High Charge and Energy Particle Radiation and Chronological Age on Biomarkers of Oxidative Stress and Tissue Degeneration: A Ground-Based Study Using the Vertebrate Laboratory Model Organism Oryzias latipes. United States. https://doi.org/10.1371/journal.pone.0111362
Zheng, Xuan, Zhang, Xinyan, Ding, Lingling, Lee, Jeffrey R., Weinberger, Paul M., and Dynan, William S. Thu .
"Synergistic Effect of High Charge and Energy Particle Radiation and Chronological Age on Biomarkers of Oxidative Stress and Tissue Degeneration: A Ground-Based Study Using the Vertebrate Laboratory Model Organism Oryzias latipes". United States. https://doi.org/10.1371/journal.pone.0111362. https://www.osti.gov/servlets/purl/1213670.
@article{osti_1213670,
title = {Synergistic Effect of High Charge and Energy Particle Radiation and Chronological Age on Biomarkers of Oxidative Stress and Tissue Degeneration: A Ground-Based Study Using the Vertebrate Laboratory Model Organism Oryzias latipes},
author = {Zheng, Xuan and Zhang, Xinyan and Ding, Lingling and Lee, Jeffrey R. and Weinberger, Paul M. and Dynan, William S.},
abstractNote = {High charge and energy (HZE) particles are a main hazard of the space radiation environment. Uncertainty regarding their health effects is a limiting factor in the design of human exploration-class space missions, that is, missions beyond low earth orbit. Previous work has shown that HZE exposure increases cancer risk and elicits other aging-like phenomena in animal models. Here, we investigate how a single exposure to HZE particle radiation, early in life, influences the subsequent age-dependent evolution of oxidative stress and appearance of degenerative tissue changes. Embryos of the laboratory model organism, Oryzias latipes (Japanese medaka fish), were exposed to HZE particle radiation at doses overlapping the range of anticipated human exposure. A separate cohort was exposed to reference c-radiation. Survival was monitored for 750 days, well beyond the median lifespan. The population was also sampled at intervals and liver tissue was subjected to histological and molecular analysis. HZE particle radiation dose and aging contributed synergistically to accumulation of lipid peroxidation products, which are a marker of chronic oxidative stress. This was mirrored by a decline in PPARGC1A mRNA, which encodes a transcriptional co-activator required for expression of oxidative stress defense genes and for mitochondrial maintenance. Consistent with chronic oxidative stress, mitochondria had an elongated and enlarged ultrastructure. Livers also had distinctive, cystic lesions. Depending on the endpoint, effects of c-rays in the same dose range were either lesser or not detected. Results provide a quantitative and qualitative framework for understanding relative contributions of HZE particle radiation exposure and aging to chronic oxidative stress and tissue degeneration.},
doi = {10.1371/journal.pone.0111362},
journal = {PLoS ONE},
number = 11,
volume = 9,
place = {United States},
year = {Thu Nov 06 00:00:00 EST 2014},
month = {Thu Nov 06 00:00:00 EST 2014}
}
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