Effect of Thiols, Zinc, and Redox Conditions on Hg Uptake in Shewanella oneidensis
Abstract
Mercury uptake in bacteria represents a key first step in the production and accumulation Of methylmercury in biota. Previous experiments with mercury methylating bacteria have shown that Hg uptake is enhanced by some thiols, in particular cysteine, and that it is an energy-dependent process through heavy Metal TA transporters. In this study, we examine Hg uptake in the nonmethylating facultative aerobe, Shewanella oneidensis, under both anaerobic and aerobic conditions. Our results demonstrate similar characteristics of the Hg uptake system to those of the Hg methylating strains: uptake is enhanced in the presence of some thiols but not others; uptake is energy dependent as evidenced by inhibition by a protonophore; and uptake is inhibited by high Zn(II) concentrations. Initial cellular uptake rates in S. oneidensis were remarkably similar under aerobic and fumarate-reducing conditions. In conclusion, these data support a similar Hg(II) uptake mechanism within the proteobacteria of accidental Hg(II) transport through heavy metal transporters with similar rates of uptake but differences in the ability to take up Hg bound to different thiols.
- Authors:
-
- Department of Geosciences, Princeton University, Princeton, New Jersey 08544, United States
- Publication Date:
- Research Org.:
- Rutgers Univ., New Brunswick, NJ (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1209997
- Alternate Identifier(s):
- OSTI ID: 1441261
- Grant/Contract Number:
- SC0006849
- Resource Type:
- Published Article
- Journal Name:
- Environmental Science and Technology
- Additional Journal Information:
- Journal Name: Environmental Science and Technology Journal Volume: 49 Journal Issue: 12; Journal ID: ISSN 0013-936X
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Szczuka, Aleksandra, Morel, François M. M., and Schaefer, Jeffra K. Effect of Thiols, Zinc, and Redox Conditions on Hg Uptake in Shewanella oneidensis. United States: N. p., 2015.
Web. doi:10.1021/acs.est.5b00676.
Szczuka, Aleksandra, Morel, François M. M., & Schaefer, Jeffra K. Effect of Thiols, Zinc, and Redox Conditions on Hg Uptake in Shewanella oneidensis. United States. https://doi.org/10.1021/acs.est.5b00676
Szczuka, Aleksandra, Morel, François M. M., and Schaefer, Jeffra K. Tue .
"Effect of Thiols, Zinc, and Redox Conditions on Hg Uptake in Shewanella oneidensis". United States. https://doi.org/10.1021/acs.est.5b00676.
@article{osti_1209997,
title = {Effect of Thiols, Zinc, and Redox Conditions on Hg Uptake in Shewanella oneidensis},
author = {Szczuka, Aleksandra and Morel, François M. M. and Schaefer, Jeffra K.},
abstractNote = {Mercury uptake in bacteria represents a key first step in the production and accumulation Of methylmercury in biota. Previous experiments with mercury methylating bacteria have shown that Hg uptake is enhanced by some thiols, in particular cysteine, and that it is an energy-dependent process through heavy Metal TA transporters. In this study, we examine Hg uptake in the nonmethylating facultative aerobe, Shewanella oneidensis, under both anaerobic and aerobic conditions. Our results demonstrate similar characteristics of the Hg uptake system to those of the Hg methylating strains: uptake is enhanced in the presence of some thiols but not others; uptake is energy dependent as evidenced by inhibition by a protonophore; and uptake is inhibited by high Zn(II) concentrations. Initial cellular uptake rates in S. oneidensis were remarkably similar under aerobic and fumarate-reducing conditions. In conclusion, these data support a similar Hg(II) uptake mechanism within the proteobacteria of accidental Hg(II) transport through heavy metal transporters with similar rates of uptake but differences in the ability to take up Hg bound to different thiols.},
doi = {10.1021/acs.est.5b00676},
journal = {Environmental Science and Technology},
number = 12,
volume = 49,
place = {United States},
year = {Tue Jun 02 00:00:00 EDT 2015},
month = {Tue Jun 02 00:00:00 EDT 2015}
}
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https://doi.org/10.1021/acs.est.5b00676
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Works referenced in this record:
Differential protein expression in the metal-reducing bacteriumGeobacter sulfurreducens strain PCA grown with fumarate or ferric citrate
journal, January 2006
- Khare, Tripti; Esteve-Núñez, Abraham; Nevin, Kelly P.
- PROTEOMICS, Vol. 6, Issue 2
Mercury Methylation by Dissimilatory Iron-Reducing Bacteria
journal, October 2006
- Kerin, E. J.; Gilmour, C. C.; Roden, E.
- Applied and Environmental Microbiology, Vol. 72, Issue 12, p. 7919-7921
Global Transcriptome Analysis of Shewanella oneidensis MR-1 Exposed to Different Terminal Electron Acceptors
journal, September 2005
- Beliaev, A. S.; Klingeman, D. M.; Klappenbach, J. A.
- Journal of Bacteriology, Vol. 187, Issue 20
Effect of Inorganic and Organic Ligands on the Bioavailability of Methylmercury as Determined by Using a mer-lux Bioreporter
journal, August 2012
- Ndu, Udonna; Mason, Robert P.; Zhang, Huan
- Applied and Environmental Microbiology, Vol. 78, Issue 20
The impact of ionic mercury on antioxidant defenses in two mercury-sensitive anaerobic bacteria
journal, October 2013
- Wang, Yingjiao; Robison, Tyler; Wiatrowski, Heather
- BioMetals, Vol. 26, Issue 6
Effect of Divalent Metals on Hg(II) Uptake and Methylation by Bacteria
journal, February 2014
- Schaefer, Jeffra K.; Szczuka, Aleksandra; Morel, François M. M.
- Environmental Science & Technology, Vol. 48, Issue 5
A thermophilic bacterial origin and subsequent constraints by redox, light and salinity on the evolution of the microbial mercuric reductase: The natural history of the mercuric reductase
journal, November 2010
- Barkay, Tamar; Kritee, K.; Boyd, Eric
- Environmental Microbiology, Vol. 12, Issue 11
Evidence for facilitated uptake of Hg(II) by Vibrio anguillarum and Escherichia coli under anaerobic and aerobic conditions
journal, July 2002
- Golding, George R.; Kelly, Carol A.; Sparling, Richard
- Limnology and Oceanography, Vol. 47, Issue 4
High methylation rates of mercury bound to cysteine by Geobacter sulfurreducens
journal, January 2009
- Schaefer, Jeffra K.; Morel, François M. M.
- Nature Geoscience, Vol. 2, Issue 2
Sequestration of Hg 2+ by Some Biologically Important Thiols
journal, December 2011
- Cardiano, Paola; Falcone, Gabriella; Foti, Claudia
- Journal of Chemical & Engineering Data, Vol. 56, Issue 12
Syntrophs Dominate Sequences Associated with the Mercury Methylation-Related Gene hgcA in the Water Conservation Areas of the Florida Everglades
journal, August 2014
- Bae, Hee-Sung; Dierberg, Forrest E.; Ogram, Andrew
- Applied and Environmental Microbiology, Vol. 80, Issue 20
Mercury Methylation by the Methanogen Methanospirillum hungatei
journal, August 2013
- Yu, Ri-Qing; Reinfelder, John R.; Hines, Mark E.
- Applied and Environmental Microbiology, Vol. 79, Issue 20
Unexpected Effects of Gene Deletion on Interactions of Mercury with the Methylation-Deficient Mutant Δ hgcAB
journal, April 2014
- Lin, Hui; Hurt, Richard A.; Johs, Alexander
- Environmental Science & Technology Letters, Vol. 1, Issue 5
Analysis of the Microbial Community Structure by Monitoring an Hg Methylation Gene (hgcA) in Paddy Soils along an Hg Gradient
journal, February 2014
- Liu, Y. -R.; Yu, R. -Q.; Zheng, Y. -M.
- Applied and Environmental Microbiology, Vol. 80, Issue 9
Mercury Methylation by Novel Microorganisms from New Environments
journal, September 2013
- Gilmour, Cynthia C.; Podar, Mircea; Bullock, Allyson L.
- Environmental Science & Technology, Vol. 47, Issue 20
Bacterial mercury resistance from atoms to ecosystems
journal, June 2003
- Barkay, Tamar; Miller, Susan M.; Summers, Anne O.
- FEMS Microbiology Reviews, Vol. 27, Issue 2-3
Effect of pH on Intracellular Accumulation of Trace Concentrations of Hg(II) in Escherichia coli under Anaerobic Conditions, as Measured Using a mer-lux Bioreporter
journal, February 2008
- Golding, George R.; Sparling, Richard; Kelly, Carol A.
- Applied and Environmental Microbiology, Vol. 74, Issue 3
Sulfate-Reducing Bacterium Desulfovibrio desulfuricans ND132 as a Model for Understanding Bacterial Mercury Methylation
journal, April 2011
- Gilmour, Cynthia C.; Elias, Dwayne A.; Kucken, Amy M.
- Applied and Environmental Microbiology, Vol. 77, Issue 12, p. 3938-3951
Hg( ii ) bacterial biouptake: the role of anthropogenic and biogenic ligands present in solution and spectroscopic evidence of ligand exchange reactions at the cell surface
journal, January 2014
- Thomas, Sara Anne; Tong, Tiezheng; Gaillard, Jean-François
- Metallomics, Vol. 6, Issue 12
Detection of a key Hg methylation gene, hgcA , in wetland soils : Detection of the Hg methylation gene, hgcA, in soils
journal, January 2014
- Schaefer, J. K.; Kronberg, R. -M.; Morel, F. M. M.
- Environmental Microbiology Reports, Vol. 6, Issue 5
Active transport, substrate specificity, and methylation of Hg(II) in anaerobic bacteria
journal, May 2011
- Schaefer, J. K.; Rocks, S. S.; Zheng, W.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 21
Bacterial Manganese Reduction and Growth with Manganese Oxide as the Sole Electron Acceptor
journal, June 1988
- Myers, C. R.; Nealson, K. H.
- Science, Vol. 240, Issue 4857
Determination of volatile mercury species at the picogram level by low-temperature gas chromatography with cold-vapour atomic fluorescence detection
journal, January 1988
- Bloom, Nicolas; Fitzgerald, William F.
- Analytica Chimica Acta, Vol. 208
The Genetic Basis for Bacterial Mercury Methylation
journal, February 2013
- Parks, J. M.; Johs, A.; Podar, M.
- Science, Vol. 339, Issue 6125, p. 1332-1335
Metal Chelates of Some Sulfur-containing Amino Acids *
journal, June 1964
- Lenz, G. R.; Martell, A. E.
- Biochemistry, Vol. 3, Issue 6
Nuclear magnetic resonance studies of the solution chemistry of metal complexes. 25. Hg(thiol)3 complexes and HG(II)-thiol ligand exchange kinetics
journal, September 1988
- Cheesman, Bruce V.; Arnold, Alan P.; Rabenstein, Dallas L.
- Journal of the American Chemical Society, Vol. 110, Issue 19