Porous polymer monolithic columns with gold nanoparticles as an intermediate ligand for the separation of proteins in reverse phase-ion exchange mixed mode

Terborg, Lydia; Masini, Jorge C.; Lin, Michelle; Lipponen, Katriina; Riekolla, Marja-Liisa; Svec, Frantisek

doi:10.1016/j.jare.2014.10.004

Title: Porous polymer monolithic columns with gold nanoparticles as an intermediate ligand for the separation of proteins in reverse phase-ion exchange mixed mode

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Abstract

A new approach has been developed for the preparation of mixed-mode stationary phases to separate proteins. The pore surface of monolithic poly(glycidyl methacrylate-co-ethylene dimethacrylate) capillary columns was functionalized with thiols and coated with gold nanoparticles. The final mixed mode surface chemistry was formed by attaching, in a single step, alkanethiols, mercaptoalkanoic acids, and their mixtures on the free surface of attached gold nanoparticles. Use of these mixtures allowed fine tuning of the hydrophobic/hydrophilic balance. The amount of attached gold nanoparticles according to thermal gravimetric analysis was 44.8 wt.%. This value together with results of frontal elution enabled calculation of surface coverage with the alkanethiol and mercaptoalkanoic acid ligands. Interestingly, alkanethiols coverage in a range of 4.46–4.51 molecules/nm² significantly exceeded that of mercaptoalkanoic acids with 2.39–2.45 molecules/nm². The mixed mode character of these monolithic stationary phases was for the first time demonstrated in the separations of proteins that could be achieved in the same column using gradient elution conditions typical of reverse phase (using gradient of acetonitrile in water) and ion exchange chromatographic modes (applying gradient of salt in water), respectively.

Authors:: Terborg, Lydia; Masini, Jorge C.; Lin, Michelle; Lipponen, Katriina; Riekolla, Marja-Liisa; Svec, Frantisek

Publication Date:: Fri May 01 00:00:00 EDT 2015

Research Org.:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1241556

Alternate Identifier(s):: OSTI ID: 1208643

Grant/Contract Number:: AC02-05CH11231

Resource Type:: Published Article

Journal Name:: Journal of Advanced Research

Additional Journal Information:: Journal Name: Journal of Advanced Research Journal Volume: 6 Journal Issue: 3; Journal ID: ISSN 2090-1232

Publisher:: Elsevier

Country of Publication:: Egypt

Language:: English

Subject:: 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; gold nanoparticles; mixed mode; monolith; proteins; separation

Citation Formats


                    Terborg, Lydia, Masini, Jorge C., Lin, Michelle, Lipponen, Katriina, Riekolla, Marja-Liisa, and Svec, Frantisek. Porous polymer monolithic columns with gold nanoparticles as an intermediate ligand for the separation of proteins in reverse phase-ion exchange mixed mode.  Egypt: N. p., 2015. 
Web.  doi:10.1016/j.jare.2014.10.004.

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                    Terborg, Lydia, Masini, Jorge C., Lin, Michelle, Lipponen, Katriina, Riekolla, Marja-Liisa, & Svec, Frantisek. Porous polymer monolithic columns with gold nanoparticles as an intermediate ligand for the separation of proteins in reverse phase-ion exchange mixed mode.  Egypt.  https://doi.org/10.1016/j.jare.2014.10.004

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                    Terborg, Lydia, Masini, Jorge C., Lin, Michelle, Lipponen, Katriina, Riekolla, Marja-Liisa, and Svec, Frantisek. Fri .  
"Porous polymer monolithic columns with gold nanoparticles as an intermediate ligand for the separation of proteins in reverse phase-ion exchange mixed mode".  Egypt.  https://doi.org/10.1016/j.jare.2014.10.004.

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@article{osti_1241556,

  title        = {Porous polymer monolithic columns with gold nanoparticles as an intermediate ligand for the separation of proteins in reverse phase-ion exchange mixed mode},

  author       = {Terborg, Lydia and Masini, Jorge C. and Lin, Michelle and Lipponen, Katriina and Riekolla, Marja-Liisa and Svec, Frantisek},

  abstractNote = {A new approach has been developed for the preparation of mixed-mode stationary phases to separate proteins. The pore surface of monolithic poly(glycidyl methacrylate-co-ethylene dimethacrylate) capillary columns was functionalized with thiols and coated with gold nanoparticles. The final mixed mode surface chemistry was formed by attaching, in a single step, alkanethiols, mercaptoalkanoic acids, and their mixtures on the free surface of attached gold nanoparticles. Use of these mixtures allowed fine tuning of the hydrophobic/hydrophilic balance. The amount of attached gold nanoparticles according to thermal gravimetric analysis was 44.8 wt.%. This value together with results of frontal elution enabled calculation of surface coverage with the alkanethiol and mercaptoalkanoic acid ligands. Interestingly, alkanethiols coverage in a range of 4.46–4.51 molecules/nm2 significantly exceeded that of mercaptoalkanoic acids with 2.39–2.45 molecules/nm2. The mixed mode character of these monolithic stationary phases was for the first time demonstrated in the separations of proteins that could be achieved in the same column using gradient elution conditions typical of reverse phase (using gradient of acetonitrile in water) and ion exchange chromatographic modes (applying gradient of salt in water), respectively.},

  doi          = {10.1016/j.jare.2014.10.004},

  journal      = {Journal of Advanced Research},

  number       = 3,

  volume       = 6,

  place        = {Egypt},

  year         = {Fri May 01 00:00:00 EDT 2015},

  month        = {Fri May 01 00:00:00 EDT 2015}

}

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