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Title: Redox linked flavin sites in extracellular decaheme proteins involved in microbe-mineral electron transfer.

Abstract

Extracellular microbe-mineral electron transfer is a major driving force for the oxidation of organic carbon in many subsurface environments. Extracellular multi-heme cytochromes of the Shewenella genus play a major role in this process but the mechanism of electron exchange at the interface between cytochrome and acceptor is widely debated. The 1.8 Å x-ray crystal structure of the decaheme MtrC revealed a highly conserved CX₈C disulfide that, when substituted for AX₈A, severely compromised the ability of S. oneidensis to grow under aerobic conditions. Reductive cleavage of the disulfide in the presence of flavin mononucleotide (FMN) resulted in the reversible formation of a stable flavocytochrome. Similar results were also observed with other decaheme cytochromes, OmcA, MtrF and UndA. The data suggest that these decaheme cytochromes can transition between highly reactive flavocytochromes or less reactive cytochromes, and that this transition is controlled by a redox active disulfide that responds to the presence of oxygen.

Authors:
 [1];  [1];  [1];  [1];  [1];  [2];  [2];  [2];  [1];  [1];  [1]
  1. Univ. of East Anglia, Norwich (United Kingdom)
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1203147
Grant/Contract Number:  
AC05-76RL01830
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 5; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; soil microbiology; biogeochemistry; enzyme mechanisms; X-ray crystallography

Citation Formats

Edwards, Marcus J., White, Gaye F., Norman, Michael, Tome-Fernandez, Alice, Ainsworth, Emma, Shi, Liang, Fredrickson, Jim K., Zachara, John M., Butt, Julea N., Richardson, David J., and Clarke, Thomas A. Redox linked flavin sites in extracellular decaheme proteins involved in microbe-mineral electron transfer.. United States: N. p., 2015. Web. doi:10.1038/srep11677.
Edwards, Marcus J., White, Gaye F., Norman, Michael, Tome-Fernandez, Alice, Ainsworth, Emma, Shi, Liang, Fredrickson, Jim K., Zachara, John M., Butt, Julea N., Richardson, David J., & Clarke, Thomas A. Redox linked flavin sites in extracellular decaheme proteins involved in microbe-mineral electron transfer.. United States. doi:10.1038/srep11677.
Edwards, Marcus J., White, Gaye F., Norman, Michael, Tome-Fernandez, Alice, Ainsworth, Emma, Shi, Liang, Fredrickson, Jim K., Zachara, John M., Butt, Julea N., Richardson, David J., and Clarke, Thomas A. Wed . "Redox linked flavin sites in extracellular decaheme proteins involved in microbe-mineral electron transfer.". United States. doi:10.1038/srep11677. https://www.osti.gov/servlets/purl/1203147.
@article{osti_1203147,
title = {Redox linked flavin sites in extracellular decaheme proteins involved in microbe-mineral electron transfer.},
author = {Edwards, Marcus J. and White, Gaye F. and Norman, Michael and Tome-Fernandez, Alice and Ainsworth, Emma and Shi, Liang and Fredrickson, Jim K. and Zachara, John M. and Butt, Julea N. and Richardson, David J. and Clarke, Thomas A.},
abstractNote = {Extracellular microbe-mineral electron transfer is a major driving force for the oxidation of organic carbon in many subsurface environments. Extracellular multi-heme cytochromes of the Shewenella genus play a major role in this process but the mechanism of electron exchange at the interface between cytochrome and acceptor is widely debated. The 1.8 Å x-ray crystal structure of the decaheme MtrC revealed a highly conserved CX₈C disulfide that, when substituted for AX₈A, severely compromised the ability of S. oneidensis to grow under aerobic conditions. Reductive cleavage of the disulfide in the presence of flavin mononucleotide (FMN) resulted in the reversible formation of a stable flavocytochrome. Similar results were also observed with other decaheme cytochromes, OmcA, MtrF and UndA. The data suggest that these decaheme cytochromes can transition between highly reactive flavocytochromes or less reactive cytochromes, and that this transition is controlled by a redox active disulfide that responds to the presence of oxygen.},
doi = {10.1038/srep11677},
journal = {Scientific Reports},
number = ,
volume = 5,
place = {United States},
year = {2015},
month = {7}
}

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