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Title: Protein Viability on Au Nanoparticles during an Electrospray and Electrostatic-Force-Directed Assembly Process

Abstract

We study the protein viability on Au nanoparticles during an electrospray and electrostatic-force-directed assembly process, through which Au nanoparticle-antibody conjugates are assembled onto the surface of carbon nanotubes (CNTs) to fabricate carbon nanotube field-effect transistor (CNTFET) biosensors. Enzyme-linked immunosorbent assay (ELISA) and field-effect transistor (FET) measurements have been used to investigate the antibody activity after the nanoparticle assembly. Upon the introduction of matching antigens, the colored reaction from the ELISA and the change in the electrical characteristic of the CNTFET device confirm that the antibody activity is preserved during the assembly process.

Authors:
 [1];  [1];  [1];  [2]
  1. Department of Mechanical Engineering, University of Wisconsin-Milwaukee, 3200 North Cramer Street, Milwaukee, WI 53211, USA
  2. Department of Mechanical Engineering, University of Wisconsin-Milwaukee, 3200 North Cramer Street, Milwaukee, WI 53211, USA, State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, The Institute for Advanced Materials and Nano Biomedicine, Tongji University, 1239 Siping Road, Shanghai 200092, China
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1198459
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Published Article
Journal Name:
Journal of Nanomaterials
Additional Journal Information:
Journal Name: Journal of Nanomaterials Journal Volume: 2010; Journal ID: ISSN 1687-4110
Publisher:
Hindawi Publishing Corporation
Country of Publication:
Egypt
Language:
English

Citation Formats

Mao, Shun, Lu, Ganhua, Yu, Kehan, and Chen, Junhong. Protein Viability on Au Nanoparticles during an Electrospray and Electrostatic-Force-Directed Assembly Process. Egypt: N. p., 2010. Web. doi:10.1155/2010/196393.
Mao, Shun, Lu, Ganhua, Yu, Kehan, & Chen, Junhong. Protein Viability on Au Nanoparticles during an Electrospray and Electrostatic-Force-Directed Assembly Process. Egypt. doi:10.1155/2010/196393.
Mao, Shun, Lu, Ganhua, Yu, Kehan, and Chen, Junhong. Fri . "Protein Viability on Au Nanoparticles during an Electrospray and Electrostatic-Force-Directed Assembly Process". Egypt. doi:10.1155/2010/196393.
@article{osti_1198459,
title = {Protein Viability on Au Nanoparticles during an Electrospray and Electrostatic-Force-Directed Assembly Process},
author = {Mao, Shun and Lu, Ganhua and Yu, Kehan and Chen, Junhong},
abstractNote = {We study the protein viability on Au nanoparticles during an electrospray and electrostatic-force-directed assembly process, through which Au nanoparticle-antibody conjugates are assembled onto the surface of carbon nanotubes (CNTs) to fabricate carbon nanotube field-effect transistor (CNTFET) biosensors. Enzyme-linked immunosorbent assay (ELISA) and field-effect transistor (FET) measurements have been used to investigate the antibody activity after the nanoparticle assembly. Upon the introduction of matching antigens, the colored reaction from the ELISA and the change in the electrical characteristic of the CNTFET device confirm that the antibody activity is preserved during the assembly process.},
doi = {10.1155/2010/196393},
journal = {Journal of Nanomaterials},
number = ,
volume = 2010,
place = {Egypt},
year = {2010},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1155/2010/196393

Citation Metrics:
Cited by: 1 work
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