Reaching the Information Limit in Cryo-EM of Biological Macromolecules: Experimental Aspects

Glaeser, Robert M.; Hall, Richard J.

doi:10.1016/j.bpj.2011.04.018

Title: Reaching the Information Limit in Cryo-EM of Biological Macromolecules: Experimental Aspects

Authors:: Glaeser, Robert M.; Hall, Richard J.

Publication Date:: Sun May 01 00:00:00 EDT 2011

Sponsoring Org.:: USDOE

OSTI Identifier:: 1198424

Grant/Contract Number:: AC02-05CH11231

Resource Type:: Published Article

Journal Name:: Biophysical Journal

Additional Journal Information:: Journal Name: Biophysical Journal Journal Volume: 100 Journal Issue: 10; Journal ID: ISSN 0006-3495

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Citation Formats


                    Glaeser, Robert M., and Hall, Richard J. Reaching the Information Limit in Cryo-EM of Biological Macromolecules: Experimental Aspects.  United States: N. p., 2011. 
Web.  doi:10.1016/j.bpj.2011.04.018.

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                    Glaeser, Robert M., & Hall, Richard J. Reaching the Information Limit in Cryo-EM of Biological Macromolecules: Experimental Aspects.  United States.  https://doi.org/10.1016/j.bpj.2011.04.018

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                    Glaeser, Robert M., and Hall, Richard J. Sun .  
"Reaching the Information Limit in Cryo-EM of Biological Macromolecules: Experimental Aspects".  United States.  https://doi.org/10.1016/j.bpj.2011.04.018.

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@article{osti_1198424,

  title        = {Reaching the Information Limit in Cryo-EM of Biological Macromolecules: Experimental Aspects},

  author       = {Glaeser, Robert M. and Hall, Richard J.},

  abstractNote = {},

  doi          = {10.1016/j.bpj.2011.04.018},

  journal      = {Biophysical Journal},

  number       = 10,

  volume       = 100,

  place        = {United States},

  year         = {Sun May 01 00:00:00 EDT 2011},

  month        = {Sun May 01 00:00:00 EDT 2011}

}

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Works referenced in this record:

Characterisation of a CMOS active pixel sensor for use in the TEAM microscope
journal, October 2010

Battaglia, Marco; Contarato, Devis; Denes, Peter
Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 622, Issue 3
DOI: 10.1016/j.nima.2010.07.066

Zernike Phase Plate Cryoelectron Microscopy Facilitates Single Particle Analysis of Unstained Asymmetric Protein Complexes
journal, January 2010

Chang, Wei-Hau; Chiu, Michael T. -K.; Chen, Chin-Yu
Structure, Vol. 18, Issue 1
DOI: 10.1016/j.str.2009.12.001

Using cryo-EM to measure the dipole potential of a lipid membrane
journal, November 2006

Wang, L.; Bose, P. S.; Sigworth, F. J.
Proceedings of the National Academy of Sciences, Vol. 103, Issue 49
DOI: 10.1073/pnas.0608714103

Design of a microfabricated, two-electrode phase-contrast element suitable for electron microscopy
journal, April 2007

Cambie, Rossana; Downing, Kenneth H.; Typke, Dieter
Ultramicroscopy, Vol. 107, Issue 4-5
DOI: 10.1016/j.ultramic.2006.09.001

Single particle analysis based on Zernike phase contrast transmission electron microscopy
journal, February 2008

Danev, Radostin; Nagayama, Kuniaki
Journal of Structural Biology, Vol. 161, Issue 2
DOI: 10.1016/j.jsb.2007.10.015

Ribosome-induced changes in elongation factor Tu conformation control GTP hydrolysis
journal, January 2009

Villa, E.; Sengupta, J.; Trabuco, L. G.
Proceedings of the National Academy of Sciences, Vol. 106, Issue 4
DOI: 10.1073/pnas.0811370106

Fabrication of a Boersch phase plate for phase contrast imaging in a transmission electron microscope
journal, March 2006

Schultheiß, K.; Pérez-Willard, F.; Barton, B.
Review of Scientific Instruments, Vol. 77, Issue 3
DOI: 10.1063/1.2179411

Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution
journal, October 2010

Armache, J. -P.; Jarasch, A.; Anger, A. M.
Proceedings of the National Academy of Sciences, Vol. 107, Issue 46
DOI: 10.1073/pnas.1009999107

Automatic CTF correction for single particles based upon multivariate statistical analysis of individual power spectra
journal, June 2003

Sander, B.; Golas, M. M.; Stark, H.
Journal of Structural Biology, Vol. 142, Issue 3
DOI: 10.1016/S1047-8477(03)00072-8

The resolution dependence of optimal exposures in liquid nitrogen temperature electron cryomicroscopy of catalase crystals
journal, March 2010

Baker, Lindsay A.; Smith, Eric A.; Bueler, Stephanie A.
Journal of Structural Biology, Vol. 169, Issue 3
DOI: 10.1016/j.jsb.2009.11.014

How I Discovered Phase Contrast
journal, March 1955

Zernike, F.
Science, Vol. 121, Issue 3141
DOI: 10.1126/science.121.3141.345

Some remarks concerning the influence of electron noise on 3D reconstruction
journal, January 1981

Hoppe, W.; Hegerl, R.
Ultramicroscopy, Vol. 6, Issue 1
DOI: 10.1016/S0304-3991(81)80200-8

Development of phase plates for electron microscopes and their biological application
journal, February 2008

Nagayama, Kuniaki
European Biophysics Journal, Vol. 37, Issue 4
DOI: 10.1007/s00249-008-0264-5

Towards atomic resolution structural determination by single-particle cryo-electron microscopy
journal, April 2008

Zhou, Z. Hong
Current Opinion in Structural Biology, Vol. 18, Issue 2
DOI: 10.1016/j.sbi.2008.03.004

Optimizing phase contrast in transmission electron microscopy with an electrostatic (Boersch) phase plate
journal, February 2007

Majorovits, E.; Barton, B.; Schultheiß, K.
Ultramicroscopy, Vol. 107, Issue 2-3
DOI: 10.1016/j.ultramic.2006.07.006

Über die Kontraste von Atomen im Elektronenmikroskop
journal, December 1947

Boersch, Hans
Zeitschrift für Naturforschung A, Vol. 2, Issue 11-12
DOI: 10.1515/zna-1947-11-1204

Phase contrast electron microscopy: development of thin-film phase plates and biological applications
journal, March 2008

Nagayama, Kuniaki; Danev, Radostin
Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 363, Issue 1500
DOI: 10.1098/rstb.2008.2268

The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules
journal, May 1995

Henderson, Richard
Quarterly Reviews of Biophysics, Vol. 28, Issue 2
DOI: 10.1017/S003358350000305X

GTPase activation of elongation factor EF-Tu by the ribosome during decoding
journal, February 2009

Schuette, Jan-Christian; Murphy, Frank V.; Kelley, Ann C.
The EMBO Journal, Vol. 28, Issue 6
DOI: 10.1038/emboj.2009.26

Mechanism of folding chamber closure in a group II chaperonin
journal, January 2010

Zhang, Junjie; Baker, Matthew L.; Schröder, Gunnar F.
Nature, Vol. 463, Issue 7279
DOI: 10.1038/nature08701

Structural basis for scaffolding-mediated assembly and maturation of a dsDNA virus
journal, January 2011

Chen, D. -H.; Baker, M. L.; Hryc, C. F.
Proceedings of the National Academy of Sciences, Vol. 108, Issue 4
DOI: 10.1073/pnas.1015739108

De Novo Backbone Trace of GroEL from Single Particle Electron Cryomicroscopy
journal, March 2008

Ludtke, Steven J.; Baker, Matthew L.; Chen, Dong-Hua
Structure, Vol. 16, Issue 3
DOI: 10.1016/j.str.2008.02.007

4.0-A resolution cryo-EM structure of the mammalian chaperonin TRiC/CCT reveals its unique subunit arrangement
journal, March 2010

Cong, Y.; Baker, M. L.; Jakana, J.
Proceedings of the National Academy of Sciences, Vol. 107, Issue 11
DOI: 10.1073/pnas.0913774107

Retrospective: Radiation damage and its associated “Information Limitations”
journal, September 2008

Glaeser, Robert M.
Journal of Structural Biology, Vol. 163, Issue 3
DOI: 10.1016/j.jsb.2008.06.001

Subunit interactions in bovine papillomavirus
journal, March 2010

Wolf, M.; Garcea, R. L.; Grigorieff, N.
Proceedings of the National Academy of Sciences, Vol. 107, Issue 14
DOI: 10.1073/pnas.0914604107

Visualizing cells at the nanoscale
journal, February 2009

Leis, Andrew; Rockel, Beate; Andrees, Lars
Trends in Biochemical Sciences, Vol. 34, Issue 2
DOI: 10.1016/j.tibs.2008.10.011

Refined Structure of the Nicotinic Acetylcholine Receptor at 4Å Resolution
journal, March 2005

Unwin, Nigel
Journal of Molecular Biology, Vol. 346, Issue 4
DOI: 10.1016/j.jmb.2004.12.031

Zernike Phase Contrast Cryo-Electron Microscopy and Tomography for Structure Determination at Nanometer and Subnanometer Resolutions
journal, August 2010

Murata, Kazuyoshi; Liu, Xiangan; Danev, Radostin
Structure, Vol. 18, Issue 8
DOI: 10.1016/j.str.2010.06.006

Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy
journal, July 2010

Fischer, Niels; Konevega, Andrey L.; Wintermeyer, Wolfgang
Nature, Vol. 466, Issue 7304
DOI: 10.1038/nature09206

3.88 Å structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy
journal, April 2008

Yu, Xuekui; Jin, Lei; Zhou, Z. Hong
Nature, Vol. 453, Issue 7193
DOI: 10.1038/nature06893

Exploring conformational modes of macromolecular assemblies by multiparticle cryo-EM
journal, October 2009

Spahn, Christian MT; Penczek, Pawel A.
Current Opinion in Structural Biology, Vol. 19, Issue 5
DOI: 10.1016/j.sbi.2009.08.001

Images of paraffin monolayer crystals with perfect contrast: Minimization of beam-induced specimen motion
journal, January 2011

Glaeser, R. M.; McMullan, G.; Faruqi, A. R.
Ultramicroscopy, Vol. 111, Issue 2
DOI: 10.1016/j.ultramic.2010.10.010

Practical factors affecting the performance of a thin-film phase plate for transmission electron microscopy
journal, March 2009

Danev, Radostin; Glaeser, Robert M.; Nagayama, Kuniaki
Ultramicroscopy, Vol. 109, Issue 4
DOI: 10.1016/j.ultramic.2008.12.006

Optimal Determination of Particle Orientation, Absolute Hand, and Contrast Loss in Single-particle Electron Cryomicroscopy
journal, October 2003

Rosenthal, Peter B.; Henderson, Richard
Journal of Molecular Biology, Vol. 333, Issue 4
DOI: 10.1016/j.jmb.2003.07.013

Zernike phase contrast cryo-electron tomography
journal, August 2010

Danev, Radostin; Kanamaru, Shuji; Marko, Michael
Journal of Structural Biology, Vol. 171, Issue 2
DOI: 10.1016/j.jsb.2010.03.013

Spot-scan imaging in transmission electron microscopy
journal, January 1991

Downing, K.
Science, Vol. 251, Issue 4989
DOI: 10.1126/science.1846047

Contrast transfer for frozen-hydrated specimens: Determination from pairs of defocused images
journal, January 1988

Toyoshima, Chikashi; Unwin, Nigel
Ultramicroscopy, Vol. 25, Issue 4
DOI: 10.1016/0304-3991(88)90003-4

Visualizing Ribosome Biogenesis: Parallel Assembly Pathways for the 30 S Subunit
journal, October 2010

Mulder, Anke M.; Yoshioka, Craig; Beck, Andrea H.
Science, Vol. 330, Issue 6004
DOI: 10.1126/science.1193220

Detective quantum efficiency of electron area detectors in electron microscopy
journal, August 2009

McMullan, G.; Chen, S.; Henderson, R.
Ultramicroscopy, Vol. 109, Issue 9
DOI: 10.1016/j.ultramic.2009.04.002

Cryo-electron microscopy of biological nanostructures
journal, January 2008

Glaeser, Robert M.
Physics Today, Vol. 61, Issue 1
DOI: 10.1063/1.2835153

Electron energy filtering significantly improves amplitude contrast of frozen-hydrated protein at 300kV
journal, December 2006

Yonekura, Koji; Braunfeld, Michael B.; Maki-Yonekura, Saori
Journal of Structural Biology, Vol. 156, Issue 3
DOI: 10.1016/j.jsb.2006.07.016

Quantitative analysis of image contrast in electron micrographs of beam-sensitive crystals
journal, January 1985

Henderson, Richard; Glaeser, Robert M.
Ultramicroscopy, Vol. 16, Issue 2
DOI: 10.1016/0304-3991(85)90069-5

Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy
journal, August 2003

Yonekura, Koji; Maki-Yonekura, Saori; Namba, Keiichi
Nature, Vol. 424, Issue 6949
DOI: 10.1038/nature01830

Electron Microscopy of Biological Macromolecules: Bridging the Gap between What Physics Allows and What We Currently Can Get
journal, January 2004

Typke, Dieter; Downing, Kenneth H.; Glaeser, Robert M.
Microscopy and Microanalysis, Vol. 10, Issue 1
DOI: 10.1017/S1431927604040164

Cryomesh™: A New Substrate for Cryo-Electron Microscopy
journal, January 2010

Yoshioka, Craig; Carragher, Bridget; Potter, Clinton S.
Microscopy and Microanalysis, Vol. 16, Issue 1
DOI: 10.1017/S1431927609991310

Molecular interactions in rotavirus assembly and uncoating seen by high-resolution cryo-EM
journal, June 2009

Chen, J. Z.; Settembre, E. C.; Aoki, S. T.
Proceedings of the National Academy of Sciences, Vol. 106, Issue 26
DOI: 10.1073/pnas.0904024106

Backbone structure of the infectious ε15 virus capsid revealed by electron cryomicroscopy
journal, February 2008

Jiang, Wen; Baker, Matthew L.; Jakana, Joanita
Nature, Vol. 451, Issue 7182
DOI: 10.1038/nature06665

The relevance of dose-fractionation in tomography of radiation-sensitive specimens
journal, October 1995

McEwen, Bruce F.; Downing, Kenneth H.; Glaeser, Robert M.
Ultramicroscopy, Vol. 60, Issue 3
DOI: 10.1016/0304-3991(95)00082-8

Lipid–protein interactions in double-layered two-dimensional AQP0 crystals
journal, December 2005

Gonen, Tamir; Cheng, Yifan; Sliz, Piotr
Nature, Vol. 438, Issue 7068
DOI: 10.1038/nature04321

Refined structure of αβ-tubulin at 3.5 Å resolution 1 1Edited by I. A. Wilson
journal, November 2001

Löwe, J.; Li, H.; Downing, K. H.
Journal of Molecular Biology, Vol. 313, Issue 5
DOI: 10.1006/jmbi.2001.5077

Use of spot-scan procedure for recording low-dose micrographs of beam-sensitive specimens
journal, January 1987

Bullough, Per; Henderson, Richard
Ultramicroscopy, Vol. 21, Issue 3
DOI: 10.1016/0304-3991(87)90147-1

Review: Electron Crystallography: Present Excitement, a Nod to the Past, Anticipating the Future
journal, December 1999

Glaeser, Robert M.
Journal of Structural Biology, Vol. 128, Issue 1
DOI: 10.1006/jsbi.1999.4172

Atomic Structure of Human Adenovirus by Cryo-EM Reveals Interactions Among Protein Networks
journal, August 2010

Liu, H.; Jin, L.; Koh, S. B. S.
Science, Vol. 329, Issue 5995
DOI: 10.1126/science.1187433

Equivalent Mutations in the Eight Subunits of the Chaperonin CCT Produce Dramatically Different Cellular and Gene Expression Phenotypes
journal, August 2010

Amit, Maya; Weisberg, Sarah J.; Nadler-Holly, Michal
Journal of Molecular Biology, Vol. 401, Issue 3
DOI: 10.1016/j.jmb.2010.06.037

Quantum noise in 2D projections and 3D reconstructions
journal, January 1981

Saxberg, B. E. H.; Saxton, W. O.
Ultramicroscopy, Vol. 6, Issue 1
DOI: 10.1016/S0304-3991(81)80182-9

3.3 Å Cryo-EM Structure of a Nonenveloped Virus Reveals a Priming Mechanism for Cell Entry
journal, April 2010

Zhang, Xing; Jin, Lei; Fang, Qin
Cell, Vol. 141, Issue 3
DOI: 10.1016/j.cell.2010.03.041

Modeling and Analysis of Charged-Particle CMOS Image Sensor Arrays
journal, June 2009

Li, Shengdong; Matis, Howard S.; Xuong, Nguyen-Huu
IEEE Transactions on Nuclear Science, Vol. 56, Issue 3
DOI: 10.1109/TNS.2009.2015303

Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction
journal, January 2008

Zhang, X.; Settembre, E.; Xu, C.
Proceedings of the National Academy of Sciences, Vol. 105, Issue 6
DOI: 10.1073/pnas.0711623105

Looking Inside Adenovirus
journal, August 2010

Harrison, Stephen C.
Science, Vol. 329, Issue 5995
DOI: 10.1126/science.1194922

Radiation damage effects at four specimen temperatures from 4 to 100K
journal, March 2010

Bammes, Benjamin E.; Jakana, Joanita; Schmid, Michael F.
Journal of Structural Biology, Vol. 169, Issue 3
DOI: 10.1016/j.jsb.2009.11.001

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Title: Reaching the Information Limit in Cryo-EM of Biological Macromolecules: Experimental Aspects

Citation Formats

Characterisation of a CMOS active pixel sensor for use in the TEAM microscope journal, October 2010

Zernike Phase Plate Cryoelectron Microscopy Facilitates Single Particle Analysis of Unstained Asymmetric Protein Complexes journal, January 2010

Using cryo-EM to measure the dipole potential of a lipid membrane journal, November 2006

Design of a microfabricated, two-electrode phase-contrast element suitable for electron microscopy journal, April 2007

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Automatic CTF correction for single particles based upon multivariate statistical analysis of individual power spectra journal, June 2003

The resolution dependence of optimal exposures in liquid nitrogen temperature electron cryomicroscopy of catalase crystals journal, March 2010

How I Discovered Phase Contrast journal, March 1955

Some remarks concerning the influence of electron noise on 3D reconstruction journal, January 1981

Development of phase plates for electron microscopes and their biological application journal, February 2008

Towards atomic resolution structural determination by single-particle cryo-electron microscopy journal, April 2008

Optimizing phase contrast in transmission electron microscopy with an electrostatic (Boersch) phase plate journal, February 2007

Über die Kontraste von Atomen im Elektronenmikroskop journal, December 1947

Phase contrast electron microscopy: development of thin-film phase plates and biological applications journal, March 2008

The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules journal, May 1995

GTPase activation of elongation factor EF-Tu by the ribosome during decoding journal, February 2009

Mechanism of folding chamber closure in a group II chaperonin journal, January 2010

Structural basis for scaffolding-mediated assembly and maturation of a dsDNA virus journal, January 2011

De Novo Backbone Trace of GroEL from Single Particle Electron Cryomicroscopy journal, March 2008

4.0-A resolution cryo-EM structure of the mammalian chaperonin TRiC/CCT reveals its unique subunit arrangement journal, March 2010

Retrospective: Radiation damage and its associated “Information Limitations” journal, September 2008

Subunit interactions in bovine papillomavirus journal, March 2010

Visualizing cells at the nanoscale journal, February 2009

Refined Structure of the Nicotinic Acetylcholine Receptor at 4Å Resolution journal, March 2005

Zernike Phase Contrast Cryo-Electron Microscopy and Tomography for Structure Determination at Nanometer and Subnanometer Resolutions journal, August 2010

Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy journal, July 2010

3.88 Å structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy journal, April 2008

Exploring conformational modes of macromolecular assemblies by multiparticle cryo-EM journal, October 2009

Images of paraffin monolayer crystals with perfect contrast: Minimization of beam-induced specimen motion journal, January 2011

Practical factors affecting the performance of a thin-film phase plate for transmission electron microscopy journal, March 2009

Optimal Determination of Particle Orientation, Absolute Hand, and Contrast Loss in Single-particle Electron Cryomicroscopy journal, October 2003

Zernike phase contrast cryo-electron tomography journal, August 2010

Spot-scan imaging in transmission electron microscopy journal, January 1991

Contrast transfer for frozen-hydrated specimens: Determination from pairs of defocused images journal, January 1988

Visualizing Ribosome Biogenesis: Parallel Assembly Pathways for the 30 S Subunit journal, October 2010

Detective quantum efficiency of electron area detectors in electron microscopy journal, August 2009

Cryo-electron microscopy of biological nanostructures journal, January 2008

Electron energy filtering significantly improves amplitude contrast of frozen-hydrated protein at 300kV journal, December 2006

Quantitative analysis of image contrast in electron micrographs of beam-sensitive crystals journal, January 1985

Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy journal, August 2003

Electron Microscopy of Biological Macromolecules: Bridging the Gap between What Physics Allows and What We Currently Can Get journal, January 2004

Cryomesh™: A New Substrate for Cryo-Electron Microscopy journal, January 2010

Molecular interactions in rotavirus assembly and uncoating seen by high-resolution cryo-EM journal, June 2009

Backbone structure of the infectious ε15 virus capsid revealed by electron cryomicroscopy journal, February 2008

The relevance of dose-fractionation in tomography of radiation-sensitive specimens journal, October 1995

Lipid–protein interactions in double-layered two-dimensional AQP0 crystals journal, December 2005

Refined structure of αβ-tubulin at 3.5 Å resolution 1 1Edited by I. A. Wilson journal, November 2001

Use of spot-scan procedure for recording low-dose micrographs of beam-sensitive specimens journal, January 1987

Review: Electron Crystallography: Present Excitement, a Nod to the Past, Anticipating the Future journal, December 1999

Atomic Structure of Human Adenovirus by Cryo-EM Reveals Interactions Among Protein Networks journal, August 2010

Equivalent Mutations in the Eight Subunits of the Chaperonin CCT Produce Dramatically Different Cellular and Gene Expression Phenotypes journal, August 2010

Quantum noise in 2D projections and 3D reconstructions journal, January 1981

3.3 Å Cryo-EM Structure of a Nonenveloped Virus Reveals a Priming Mechanism for Cell Entry journal, April 2010

Modeling and Analysis of Charged-Particle CMOS Image Sensor Arrays journal, June 2009

Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction journal, January 2008

Looking Inside Adenovirus journal, August 2010

Radiation damage effects at four specimen temperatures from 4 to 100K journal, March 2010

Characterisation of a CMOS active pixel sensor for use in the TEAM microscope
journal, October 2010

Zernike Phase Plate Cryoelectron Microscopy Facilitates Single Particle Analysis of Unstained Asymmetric Protein Complexes
journal, January 2010

Using cryo-EM to measure the dipole potential of a lipid membrane
journal, November 2006

Design of a microfabricated, two-electrode phase-contrast element suitable for electron microscopy
journal, April 2007

Single particle analysis based on Zernike phase contrast transmission electron microscopy
journal, February 2008

Ribosome-induced changes in elongation factor Tu conformation control GTP hydrolysis
journal, January 2009

Fabrication of a Boersch phase plate for phase contrast imaging in a transmission electron microscope
journal, March 2006

Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution
journal, October 2010

Automatic CTF correction for single particles based upon multivariate statistical analysis of individual power spectra
journal, June 2003

The resolution dependence of optimal exposures in liquid nitrogen temperature electron cryomicroscopy of catalase crystals
journal, March 2010

How I Discovered Phase Contrast
journal, March 1955

Some remarks concerning the influence of electron noise on 3D reconstruction
journal, January 1981

Development of phase plates for electron microscopes and their biological application
journal, February 2008

Towards atomic resolution structural determination by single-particle cryo-electron microscopy
journal, April 2008

Optimizing phase contrast in transmission electron microscopy with an electrostatic (Boersch) phase plate
journal, February 2007

Über die Kontraste von Atomen im Elektronenmikroskop
journal, December 1947

Phase contrast electron microscopy: development of thin-film phase plates and biological applications
journal, March 2008

The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules
journal, May 1995

GTPase activation of elongation factor EF-Tu by the ribosome during decoding
journal, February 2009

Mechanism of folding chamber closure in a group II chaperonin
journal, January 2010

Structural basis for scaffolding-mediated assembly and maturation of a dsDNA virus
journal, January 2011

De Novo Backbone Trace of GroEL from Single Particle Electron Cryomicroscopy
journal, March 2008

4.0-A resolution cryo-EM structure of the mammalian chaperonin TRiC/CCT reveals its unique subunit arrangement
journal, March 2010

Retrospective: Radiation damage and its associated “Information Limitations”
journal, September 2008

Subunit interactions in bovine papillomavirus
journal, March 2010

Visualizing cells at the nanoscale
journal, February 2009

Refined Structure of the Nicotinic Acetylcholine Receptor at 4Å Resolution
journal, March 2005

Zernike Phase Contrast Cryo-Electron Microscopy and Tomography for Structure Determination at Nanometer and Subnanometer Resolutions
journal, August 2010

Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy
journal, July 2010

3.88 Å structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy
journal, April 2008

Exploring conformational modes of macromolecular assemblies by multiparticle cryo-EM
journal, October 2009

Images of paraffin monolayer crystals with perfect contrast: Minimization of beam-induced specimen motion
journal, January 2011

Practical factors affecting the performance of a thin-film phase plate for transmission electron microscopy
journal, March 2009

Optimal Determination of Particle Orientation, Absolute Hand, and Contrast Loss in Single-particle Electron Cryomicroscopy
journal, October 2003

Zernike phase contrast cryo-electron tomography
journal, August 2010

Spot-scan imaging in transmission electron microscopy
journal, January 1991

Contrast transfer for frozen-hydrated specimens: Determination from pairs of defocused images
journal, January 1988

Visualizing Ribosome Biogenesis: Parallel Assembly Pathways for the 30 S Subunit
journal, October 2010

Detective quantum efficiency of electron area detectors in electron microscopy
journal, August 2009

Cryo-electron microscopy of biological nanostructures
journal, January 2008

Electron energy filtering significantly improves amplitude contrast of frozen-hydrated protein at 300kV
journal, December 2006

Quantitative analysis of image contrast in electron micrographs of beam-sensitive crystals
journal, January 1985

Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy
journal, August 2003

Electron Microscopy of Biological Macromolecules: Bridging the Gap between What Physics Allows and What We Currently Can Get
journal, January 2004

Cryomesh™: A New Substrate for Cryo-Electron Microscopy
journal, January 2010

Molecular interactions in rotavirus assembly and uncoating seen by high-resolution cryo-EM
journal, June 2009

Backbone structure of the infectious ε15 virus capsid revealed by electron cryomicroscopy
journal, February 2008

The relevance of dose-fractionation in tomography of radiation-sensitive specimens
journal, October 1995

Lipid–protein interactions in double-layered two-dimensional AQP0 crystals
journal, December 2005

Refined structure of αβ-tubulin at 3.5 Å resolution 1 1Edited by I. A. Wilson
journal, November 2001

Use of spot-scan procedure for recording low-dose micrographs of beam-sensitive specimens
journal, January 1987

Review: Electron Crystallography: Present Excitement, a Nod to the Past, Anticipating the Future
journal, December 1999

Atomic Structure of Human Adenovirus by Cryo-EM Reveals Interactions Among Protein Networks
journal, August 2010

Equivalent Mutations in the Eight Subunits of the Chaperonin CCT Produce Dramatically Different Cellular and Gene Expression Phenotypes
journal, August 2010

Quantum noise in 2D projections and 3D reconstructions
journal, January 1981

3.3 Å Cryo-EM Structure of a Nonenveloped Virus Reveals a Priming Mechanism for Cell Entry
journal, April 2010

Modeling and Analysis of Charged-Particle CMOS Image Sensor Arrays
journal, June 2009

Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction
journal, January 2008

Looking Inside Adenovirus
journal, August 2010

Radiation damage effects at four specimen temperatures from 4 to 100K
journal, March 2010