Live Cells as Dynamic Laboratories: Time Lapse Raman Spectral Microscopy of Nanoparticles with Both IgE Targeting and pH-Sensing Functions
Abstract
This review captures the use of live cells as dynamic microlaboratories through implementation of labeled nanoparticles (nanosensors) that have both sensing and targeting functions. The addition of 2,4-ε-dinitrophenol-L-lysine (DNP) as a FcεRI targeting ligand and 4-mercaptopyridine (4-MPy) as a pH-sensing ligand enables spatial and temporal monitoring of FcεRI receptors and their pH environment within the endocytic pathway. To ensure reliability, the sensor is calibrated in vivo using the ionophore nigericin and standard buffer solutions to equilibrate the external concentration with that of the cell compartments. This review highlights the nanosensors, ability to traffic and respond to pH of receptor-bound nanosensors (1) at physiological temperature versus room temperature , (2) after pharmacological treatment with bafilomycin, an ATPase pump inhibitor, or amiloride, an inhibitor of / exchange, and (3) in response to both temperature and pharmacological treatment. Whole-cell, time lapse images are demonstrated to show the ability to transform live cells into dynamic laboratories to monitor temporal and spatial endosomal pH. The versatility of these probes shows promise for future applications relevant to intracellular trafficking and intelligent drug design.
- Authors:
-
- Decision Applications Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
- Chemistry Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
- Publication Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
- OSTI Identifier:
- 1198264
- Alternate Identifier(s):
- OSTI ID: 1626204
- Grant/Contract Number:
- RP52-05NA25396; 20080001DR; AC52-06NA25396
- Resource Type:
- Published Article
- Journal Name:
- International Journal of Analytical Chemistry
- Additional Journal Information:
- Journal Name: International Journal of Analytical Chemistry Journal Volume: 2012; Journal ID: ISSN 1687-8760
- Publisher:
- Hindawi Publishing Corporation
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Chemistry
Citation Formats
Nowak-Lovato, Kristy L., and Rector, Kirk D. Live Cells as Dynamic Laboratories: Time Lapse Raman Spectral Microscopy of Nanoparticles with Both IgE Targeting and pH-Sensing Functions. United Kingdom: N. p., 2012.
Web. doi:10.1155/2012/390182.
Nowak-Lovato, Kristy L., & Rector, Kirk D. Live Cells as Dynamic Laboratories: Time Lapse Raman Spectral Microscopy of Nanoparticles with Both IgE Targeting and pH-Sensing Functions. United Kingdom. https://doi.org/10.1155/2012/390182
Nowak-Lovato, Kristy L., and Rector, Kirk D. Sun .
"Live Cells as Dynamic Laboratories: Time Lapse Raman Spectral Microscopy of Nanoparticles with Both IgE Targeting and pH-Sensing Functions". United Kingdom. https://doi.org/10.1155/2012/390182.
@article{osti_1198264,
title = {Live Cells as Dynamic Laboratories: Time Lapse Raman Spectral Microscopy of Nanoparticles with Both IgE Targeting and pH-Sensing Functions},
author = {Nowak-Lovato, Kristy L. and Rector, Kirk D.},
abstractNote = {This review captures the use of live cells as dynamic microlaboratories through implementation of labeled nanoparticles (nanosensors) that have both sensing and targeting functions. The addition of 2,4-ε-dinitrophenol-L-lysine (DNP) as a FcεRI targeting ligand and 4-mercaptopyridine (4-MPy) as a pH-sensing ligand enables spatial and temporal monitoring of FcεRI receptors and their pH environment within the endocytic pathway. To ensure reliability, the sensor is calibrated in vivo using the ionophore nigericin and standard buffer solutions to equilibrate the external [ H + ] concentration with that of the cell compartments. This review highlights the nanosensors, ability to traffic and respond to pH of receptor-bound nanosensors (1) at physiological temperature ( 37 ° C ) versus room temperature ( 25 ° C ) , (2) after pharmacological treatment with bafilomycin, an H + ATPase pump inhibitor, or amiloride, an inhibitor of Na + / H + exchange, and (3) in response to both temperature and pharmacological treatment. Whole-cell, time lapse images are demonstrated to show the ability to transform live cells into dynamic laboratories to monitor temporal and spatial endosomal pH. The versatility of these probes shows promise for future applications relevant to intracellular trafficking and intelligent drug design.},
doi = {10.1155/2012/390182},
journal = {International Journal of Analytical Chemistry},
number = ,
volume = 2012,
place = {United Kingdom},
year = {Sun Jan 01 00:00:00 EST 2012},
month = {Sun Jan 01 00:00:00 EST 2012}
}
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