Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy
Abstract
Dynamics of nucleosomes and their interactions are important for understanding the mechanism of chromatin assembly. Internucleosomal interaction is required for the formation of higher-order chromatin structures. Although H1 histone is critically involved in the process of chromatin assembly, direct internucleosomal interactions contribute to this process as well. To characterize the interactions of nucleosomes within the nucleosome array, we designed a dinucleosome and performed direct AFM imaging. The analysis of the AFM data showed dinucleosomes are very dynamic systems, enabling the nucleosomes to move in a broad range along the DNA template. Di-nucleosomes in close proximity were observed, but their population was low. The use of the zwitterionic detergent, CHAPS, increased the dynamic range of the di-nucleosome, facilitating the formation of tight di-nucleosomes. The role of CHAPS and similar natural products in chromatin structure and dynamics is also discussed.
- Authors:
-
- Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198-6025, USA, Department of High Technologies, The International Solomon University, 1b Sholudenko Street, Kyiv 01135, Ukraine
- Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198-6025, USA, Department of Application Services, Infopulse Ukraine LLC, 24 Polyova Street, Kyiv 03056, Ukraine
- Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198-6025, USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1198215
- Grant/Contract Number:
- FG02-08ER64579
- Resource Type:
- Published Article
- Journal Name:
- Journal of Amino Acids
- Additional Journal Information:
- Journal Name: Journal of Amino Acids Journal Volume: 2012; Journal ID: ISSN 2090-0104
- Publisher:
- Hindawi Publishing Corporation
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Citation Formats
Filenko, Nina A., Palets, Dmytro B., and Lyubchenko, Yuri L. Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy. Country unknown/Code not available: N. p., 2012.
Web. doi:10.1155/2012/650840.
Filenko, Nina A., Palets, Dmytro B., & Lyubchenko, Yuri L. Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy. Country unknown/Code not available. https://doi.org/10.1155/2012/650840
Filenko, Nina A., Palets, Dmytro B., and Lyubchenko, Yuri L. Tue .
"Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy". Country unknown/Code not available. https://doi.org/10.1155/2012/650840.
@article{osti_1198215,
title = {Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy},
author = {Filenko, Nina A. and Palets, Dmytro B. and Lyubchenko, Yuri L.},
abstractNote = {Dynamics of nucleosomes and their interactions are important for understanding the mechanism of chromatin assembly. Internucleosomal interaction is required for the formation of higher-order chromatin structures. Although H1 histone is critically involved in the process of chromatin assembly, direct internucleosomal interactions contribute to this process as well. To characterize the interactions of nucleosomes within the nucleosome array, we designed a dinucleosome and performed direct AFM imaging. The analysis of the AFM data showed dinucleosomes are very dynamic systems, enabling the nucleosomes to move in a broad range along the DNA template. Di-nucleosomes in close proximity were observed, but their population was low. The use of the zwitterionic detergent, CHAPS, increased the dynamic range of the di-nucleosome, facilitating the formation of tight di-nucleosomes. The role of CHAPS and similar natural products in chromatin structure and dynamics is also discussed.},
doi = {10.1155/2012/650840},
journal = {Journal of Amino Acids},
number = ,
volume = 2012,
place = {Country unknown/Code not available},
year = {Tue Oct 23 00:00:00 EDT 2012},
month = {Tue Oct 23 00:00:00 EDT 2012}
}
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