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Title: Suppression of Proinflammatory Cytokines in Functionalized Fullerene-Exposed Dermal Keratinocytes

Abstract

Initial experiments using differentially functionalized fullerenes, CD-, hexa-, and tris-, suggested a properties dependent effect on cytotoxic and proliferative responses in human skin keratinocytes. In the present study we investigated the cytokine secretion profile of dermal epithelial cells exposed to functionalized fullerenes. Keratinocyte-derived cytokines affect homing and trafficking of normal and malignant epidermal immune as well as nonimmune cells in vivo. These cytokines are critical for regulating activation, proliferation, and differentiation of epidermal cells. Our results indicate that tris- (size range <100 nm) significantly reduces inflammatory cytokine release in a dose- and time-dependent manner. In contrast CD- demonstrated a relatively pro-inflammatory cytokine response, while hexa- did not significantly perturb cytokine responses. Physical and chemical characterizations of these engineered nanomaterials suggest that the disparate biological responses observed may potentially be a function of the aggregation properties of these fullerenes.

Authors:
 [1];  [2];  [1]
  1. Biosciences Division, Los Alamos National Laboratory, M888, TA 43, HRL-2, Los Alamos, NM 87545, USA
  2. Chemistry Division, Los Alamos National Laboratory, J567, TA 46, Los Alamos, NM 87545, USA
Publication Date:
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1198176
Grant/Contract Number:  
AC52-06NA25396
Resource Type:
Published Article
Journal Name:
Journal of Nanomaterials
Additional Journal Information:
Journal Name: Journal of Nanomaterials Journal Volume: 2010; Journal ID: ISSN 1687-4110
Publisher:
Hindawi Publishing Corporation
Country of Publication:
Egypt
Language:
English

Citation Formats

Gao, Jun, Wang, Hsing-Lin, and Iyer, Rashi. Suppression of Proinflammatory Cytokines in Functionalized Fullerene-Exposed Dermal Keratinocytes. Egypt: N. p., 2010. Web. doi:10.1155/2010/416408.
Gao, Jun, Wang, Hsing-Lin, & Iyer, Rashi. Suppression of Proinflammatory Cytokines in Functionalized Fullerene-Exposed Dermal Keratinocytes. Egypt. doi:10.1155/2010/416408.
Gao, Jun, Wang, Hsing-Lin, and Iyer, Rashi. Fri . "Suppression of Proinflammatory Cytokines in Functionalized Fullerene-Exposed Dermal Keratinocytes". Egypt. doi:10.1155/2010/416408.
@article{osti_1198176,
title = {Suppression of Proinflammatory Cytokines in Functionalized Fullerene-Exposed Dermal Keratinocytes},
author = {Gao, Jun and Wang, Hsing-Lin and Iyer, Rashi},
abstractNote = {Initial experiments using differentially functionalized fullerenes, CD-, hexa-, and tris-, suggested a properties dependent effect on cytotoxic and proliferative responses in human skin keratinocytes. In the present study we investigated the cytokine secretion profile of dermal epithelial cells exposed to functionalized fullerenes. Keratinocyte-derived cytokines affect homing and trafficking of normal and malignant epidermal immune as well as nonimmune cells in vivo. These cytokines are critical for regulating activation, proliferation, and differentiation of epidermal cells. Our results indicate that tris- (size range <100 nm) significantly reduces inflammatory cytokine release in a dose- and time-dependent manner. In contrast CD- demonstrated a relatively pro-inflammatory cytokine response, while hexa- did not significantly perturb cytokine responses. Physical and chemical characterizations of these engineered nanomaterials suggest that the disparate biological responses observed may potentially be a function of the aggregation properties of these fullerenes.},
doi = {10.1155/2010/416408},
journal = {Journal of Nanomaterials},
number = ,
volume = 2010,
place = {Egypt},
year = {2010},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1155/2010/416408

Citation Metrics:
Cited by: 1 work
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