Structural Basis for Mechanical Force Regulation of the Adhesin FimH via Finger Trap-like β Sheet Twisting

Le Trong, Isolde; Aprikian, Pavel; Kidd, Brian A.; Forero-Shelton, Manu; Tchesnokova, Veronika; Rajagopal, Ponni; Rodriguez, Victoria; Interlandi, Gianluca; Klevit, Rachel; Vogel, Viola; Stenkamp, Ronald E.; Sokurenko, Evgeni V.; Thomas, Wendy E.

doi:10.1016/j.cell.2010.03.038

Title: Structural Basis for Mechanical Force Regulation of the Adhesin FimH via Finger Trap-like β Sheet Twisting

Authors:: Le Trong, Isolde; Aprikian, Pavel; Kidd, Brian A.; Forero-Shelton, Manu; Tchesnokova, Veronika; Rajagopal, Ponni; Rodriguez, Victoria; Interlandi, Gianluca; Klevit, Rachel; Vogel, Viola; Stenkamp, Ronald E.; Sokurenko, Evgeni V.; Thomas, Wendy E.

Publication Date:: Sat May 01 00:00:00 EDT 2010

Sponsoring Org.:: USDOE

OSTI Identifier:: 1198115

Grant/Contract Number:: AC02-05CH11231

Resource Type:: Published Article

Journal Name:: Cell

Additional Journal Information:: Journal Name: Cell Journal Volume: 141 Journal Issue: 4; Journal ID: ISSN 0092-8674

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Citation Formats


                    Le Trong, Isolde, Aprikian, Pavel, Kidd, Brian A., Forero-Shelton, Manu, Tchesnokova, Veronika, Rajagopal, Ponni, Rodriguez, Victoria, Interlandi, Gianluca, Klevit, Rachel, Vogel, Viola, Stenkamp, Ronald E., Sokurenko, Evgeni V., and Thomas, Wendy E. Structural Basis for Mechanical Force Regulation of the Adhesin FimH via Finger Trap-like β Sheet Twisting.  United States: N. p., 2010. 
Web.  doi:10.1016/j.cell.2010.03.038.

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                    Le Trong, Isolde, Aprikian, Pavel, Kidd, Brian A., Forero-Shelton, Manu, Tchesnokova, Veronika, Rajagopal, Ponni, Rodriguez, Victoria, Interlandi, Gianluca, Klevit, Rachel, Vogel, Viola, Stenkamp, Ronald E., Sokurenko, Evgeni V., & Thomas, Wendy E. Structural Basis for Mechanical Force Regulation of the Adhesin FimH via Finger Trap-like β Sheet Twisting.  United States.  https://doi.org/10.1016/j.cell.2010.03.038

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                    Le Trong, Isolde, Aprikian, Pavel, Kidd, Brian A., Forero-Shelton, Manu, Tchesnokova, Veronika, Rajagopal, Ponni, Rodriguez, Victoria, Interlandi, Gianluca, Klevit, Rachel, Vogel, Viola, Stenkamp, Ronald E., Sokurenko, Evgeni V., and Thomas, Wendy E. Sat .  
"Structural Basis for Mechanical Force Regulation of the Adhesin FimH via Finger Trap-like β Sheet Twisting".  United States.  https://doi.org/10.1016/j.cell.2010.03.038.

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@article{osti_1198115,

  title        = {Structural Basis for Mechanical Force Regulation of the Adhesin FimH via Finger Trap-like β Sheet Twisting},

  author       = {Le Trong, Isolde and Aprikian, Pavel and Kidd, Brian A. and Forero-Shelton, Manu and Tchesnokova, Veronika and Rajagopal, Ponni and Rodriguez, Victoria and Interlandi, Gianluca and Klevit, Rachel and Vogel, Viola and Stenkamp, Ronald E. and Sokurenko, Evgeni V. and Thomas, Wendy E.},

  abstractNote = {},

  doi          = {10.1016/j.cell.2010.03.038},

  journal      = {Cell},

  number       = 4,

  volume       = 141,

  place        = {United States},

  year         = {Sat May 01 00:00:00 EDT 2010},

  month        = {Sat May 01 00:00:00 EDT 2010}

}

Copy to clipboard

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Works referenced in this record:

Measuring Receptor/Ligand Interaction at the Single-Bond Level: Experimental and Interpretative Issues
journal, March 2002

Zhu, Cheng; Long, Mian; Chesla, Scott E.
Annals of Biomedical Engineering, Vol. 30, Issue 3
DOI: 10.1114/1.1467923

MODIP revisited: re-evaluation and refinement of an automated procedure for modeling of disulfide bonds in proteins
journal, March 2003

Dani, Vardhan S.; Ramakrishnan, C.; Varadarajan, Raghavan
Protein Engineering, Design and Selection, Vol. 16, Issue 3
DOI: 10.1093/proeng/gzg024

Integrin-like Allosteric Properties of the Catch Bond-forming FimH Adhesin of Escherichia coli
journal, March 2008

Tchesnokova, Veronika; Aprikian, Pavel; Yakovenko, Olga
Journal of Biological Chemistry, Vol. 283, Issue 12
DOI: 10.1074/jbc.M707804200

Refinement of Macromolecular Structures by the Maximum-Likelihood Method
journal, May 1997

Murshudov, G. N.; Vagin, A. A.; Dodson, E. J.
Acta Crystallographica Section D Biological Crystallography, Vol. 53, Issue 3
DOI: 10.1107/S0907444996012255

Shear-dependent ‘stick-and-roll’ adhesion of type 1 fimbriated Escherichia coli: Stick-and-roll bacterial adhesion
journal, August 2004

Thomas, Wendy E.; Nilsson, Lina M.; Forero, Manu
Molecular Microbiology, Vol. 53, Issue 5
DOI: 10.1111/j.1365-2958.2004.04226.x

Bacterial Adhesion to Target Cells Enhanced by Shear Force
journal, June 2002

Thomas, Wendy E.; Trintchina, Elena; Forero, Manu
Cell, Vol. 109, Issue 7
DOI: 10.1016/S0092-8674(02)00796-1

Rapid automated molecular replacement by evolutionary search
journal, February 1999

Kissinger, Charles R.; Gehlhaar, Daniel K.; Fogel, David B.
Acta Crystallographica Section D Biological Crystallography, Vol. 55, Issue 2
DOI: 10.1107/S0907444998012517

Intervening with Urinary Tract Infections Using Anti-Adhesives Based on the Crystal Structure of the FimH–Oligomannose-3 Complex
journal, April 2008

Wellens, Adinda; Garofalo, Corinne; Nguyen, Hien
PLoS ONE, Vol. 3, Issue 4
DOI: 10.1371/journal.pone.0002040

NMR Structure of the Escherichia coli Type 1 Pilus Subunit FimF and Its Interactions with Other Pilus Subunits
journal, January 2008

Gossert, Alvar D.; Bettendorff, Pascal; Puorger, Chasper
Journal of Molecular Biology, Vol. 375, Issue 3
DOI: 10.1016/j.jmb.2007.10.059

Catch-Bond Model Derived from Allostery Explains Force-Activated Bacterial Adhesion
journal, February 2006

Thomas, Wendy; Forero, Manu; Yakovenko, Olga
Biophysical Journal, Vol. 90, Issue 3
DOI: 10.1529/biophysj.105.066548

Structural basis of tropism of Escherichia coli to the bladder during urinary tract infection: FimH mannose-binding pocket
journal, May 2002

Hung, Chia-Suei; Bouckaert, Julie; Hung, Danielle
Molecular Microbiology, Vol. 44, Issue 4
DOI: 10.1046/j.1365-2958.2002.02915.x

Importance of Force Linkage in Mechanochemistry of Adhesion Receptors
journal, November 2006

Astrof, Nathan S.; Salas, Azucena; Shimaoka, Motomu
Biochemistry, Vol. 45, Issue 50
DOI: 10.1021/bi061566o

Type II Cadherin Ectodomain Structures: Implications for Classical Cadherin Specificity
journal, March 2006

Patel, Saurabh D.; Ciatto, Carlo; Chen, Chien Peter
Cell, Vol. 124, Issue 6
DOI: 10.1016/j.cell.2005.12.046

Interdomain Interaction in the FimH Adhesin of Escherichia coli Regulates the Affinity to Mannose
journal, June 2007

Aprikian, Pavel; Tchesnokova, Veronika; Kidd, Brian
Journal of Biological Chemistry, Vol. 282, Issue 32
DOI: 10.1074/jbc.M702037200

The structure of the rigor complex and its implications for the power stroke
journal, December 2004

Holmes, K. C.; Trentham, D. R.; Simmons, R.
Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, Vol. 359, Issue 1452
DOI: 10.1098/rstb.2004.1566

Receptor binding studies disclose a novel class of high-affinity inhibitors of the Escherichia coli FimH adhesin
journal, November 2004

Bouckaert, Julie; Berglund, Jenny; Schembri, Mark
Molecular Microbiology, Vol. 55, Issue 2
DOI: 10.1111/j.1365-2958.2004.04415.x

The CCP4 suite programs for protein crystallography
journal, September 1994

,
Acta Crystallographica Section D Biological Crystallography, Vol. 50, Issue 5, p. 760-763
DOI: 10.1107/S0907444994003112

Improved Fourier coefficients for maps using phases from partial structures with errors
journal, May 1986

Read, R. J.
Acta Crystallographica Section A Foundations of Crystallography, Vol. 42, Issue 3
DOI: 10.1107/S0108767386099622

Mechanically Activated Integrin Switch Controls α ₅ β ₁ Function
journal, January 2009

Friedland, Julie C.; Lee, Mark H.; Boettiger, David
Science, Vol. 323, Issue 5914
DOI: 10.1126/science.1168441

Linking integrin conformation to function
journal, January 2009

Askari, Janet A.; Buckley, Patrick A.; Mould, A. Paul
Journal of Cell Science, Vol. 122, Issue 2
DOI: 10.1242/jcs.018556

FimH Forms Catch Bonds That Are Enhanced by Mechanical Force Due to Allosteric Regulation
journal, April 2008

Yakovenko, Olga; Sharma, Shivani; Forero, Manu
Journal of Biological Chemistry, Vol. 283, Issue 17
DOI: 10.1074/jbc.M707815200

Crystal structures of fibronectin-binding sites from Staphylococcus aureus FnBPA in complex with fibronectin domains
journal, August 2008

Bingham, Richard J.; Rudiño-Piñera, Enrique; Meenan, Nicola A. G.
Proceedings of the National Academy of Sciences, Vol. 105, Issue 34
DOI: 10.1073/pnas.0803556105

Autoinhibitory Domains: Modular Effectors of Cellular Regulation
journal, November 2002

Pufall, Miles A.; Graves, Barbara J.
Annual Review of Cell and Developmental Biology, Vol. 18, Issue 1
DOI: 10.1146/annurev.cellbio.18.031502.133614

Infinite Kinetic Stability against Dissociation of Supramolecular Protein Complexes through Donor Strand Complementation
journal, April 2008

Puorger, Chasper; Eidam, Oliv; Capitani, Guido
Structure, Vol. 16, Issue 4
DOI: 10.1016/j.str.2008.01.013

Selection Footprint in the FimH Adhesin Shows Pathoadaptive Niche Differentiation in Escherichia coli
journal, March 2004

Sokurenko, E. V.
Molecular Biology and Evolution, Vol. 21, Issue 7
DOI: 10.1093/molbev/msh136

Structural basis for allostery in integrins and binding to fibrinogen-mimetic therapeutics
journal, September 2004

Xiao, Tsan; Takagi, Junichi; Coller, Barry S.
Nature, Vol. 432, Issue 7013
DOI: 10.1038/nature02976

Integrin Structure, Allostery, and Bidirectional Signaling
journal, November 2005

Arnaout, M. A.; Mahalingam, B.; Xiong, J. -P.
Annual Review of Cell and Developmental Biology, Vol. 21, Issue 1
DOI: 10.1146/annurev.cellbio.21.090704.151217

Weak Rolling Adhesion Enhances Bacterial Surface Colonization
journal, March 2007

Anderson, Brett N.; Ding, Albert M.; Nilsson, Lina M.
Journal of Bacteriology, Vol. 189, Issue 5
DOI: 10.1128/JB.00899-06

Force as a Facilitator of Integrin Conformational Changes during Leukocyte Arrest on Blood Vessels and Antigen-Presenting Cells
journal, January 2007

Alon, Ronen; Dustin, Michael L.
Immunity, Vol. 26, Issue 1
DOI: 10.1016/j.immuni.2007.01.002

Coot model-building tools for molecular graphics
journal, November 2004

Emsley, Paul; Cowtan, Kevin
Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132
DOI: 10.1107/S0907444904019158

Specificity of cell-cell adhesion by classical cadherins: Critical role for low-affinity dimerization through -strand swapping
journal, June 2005

Chen, C. P.; Posy, S.; Ben-Shaul, A.
Proceedings of the National Academy of Sciences, Vol. 102, Issue 24
DOI: 10.1073/pnas.0503319102

The adhesive binding site of cadherins revisited
journal, December 1999

Shan, Wei-Song; Koch, Alexander; Murray, John
Biophysical Chemistry, Vol. 82, Issue 2-3
DOI: 10.1016/S0301-4622(99)00115-5

Structure and function of laminin LG modules
journal, August 2000

Timpl, Rupert; Tisi, Dominic; Talts, Jan F.
Matrix Biology, Vol. 19, Issue 4
DOI: 10.1016/S0945-053X(00)00072-X

Structural basis for selectin mechanochemistry
journal, December 2008

Springer, T. A.
Proceedings of the National Academy of Sciences, Vol. 106, Issue 1
DOI: 10.1073/pnas.0810784105

Remodeling of the lectin–EGF-like domain interface in P- and L-selectin increases adhesiveness and shear resistance under hydrodynamic force
journal, July 2006

Phan, Uyen T.; Waldron, Travis T.; Springer, Timothy A.
Nature Immunology, Vol. 7, Issue 8
DOI: 10.1038/ni1366

SURVEY AND SUMMARY: The morph server: a standardized system for analyzing and visualizing macromolecular motions in a database framework
journal, April 2000

Krebs, W. G.
Nucleic Acids Research, Vol. 28, Issue 8
DOI: 10.1093/nar/28.8.1665

XtalView/Xfit—A Versatile Program for Manipulating Atomic Coordinates and Electron Density
journal, April 1999

McRee, Duncan E.
Journal of Structural Biology, Vol. 125, Issue 2-3
DOI: 10.1006/jsbi.1999.4094

Crystal structure of the ternary FimC-FimF _t -FimD _N complex indicates conserved pilus chaperone-subunit complex recognition by the usher FimD
journal, January 2008

Eidam, Oliv; Dworkowski, Florian S. N.; Glockshuber, Rudi
FEBS Letters, Vol. 582, Issue 5
DOI: 10.1016/j.febslet.2008.01.030

NMRPipe: A multidimensional spectral processing system based on UNIX pipes
journal, November 1995

Delaglio, Frank; Grzesiek, Stephan; Vuister, GeertenW.
Journal of Biomolecular NMR, Vol. 6, Issue 3
DOI: 10.1007/BF00197809

Elevated Shear Stress Protects Escherichia coli Cells Adhering to Surfaces via Catch Bonds from Detachment by Soluble Inhibitors
journal, April 2006

Nilsson, Lina M.; Thomas, Wendy E.; Sokurenko, Evgeni V.
Applied and Environmental Microbiology, Vol. 72, Issue 4
DOI: 10.1128/AEM.72.4.3005-3010.2006

Recent structural studies of carbohydrate-binding modules
journal, November 2006

Hashimoto, H.
Cellular and Molecular Life Sciences, Vol. 63, Issue 24
DOI: 10.1007/s00018-006-6195-3

Structural plasticity in Ig superfamily domain 4 of ICAM-1 mediates cell surface dimerization
journal, September 2007

Chen, Xuehui; Kim, Thomas Doohun; Carman, Christopher V.
Proceedings of the National Academy of Sciences, Vol. 104, Issue 39
DOI: 10.1073/pnas.0707406104

MOLREP an Automated Program for Molecular Replacement
journal, December 1997

Vagin, A.; Teplyakov, A.
Journal of Applied Crystallography, Vol. 30, Issue 6, p. 1022-1025
DOI: 10.1107/S0021889897006766

Biophysics of Catch Bonds
journal, June 2008

Thomas, Wendy E.; Vogel, Viola; Sokurenko, Evgeni
Annual Review of Biophysics, Vol. 37, Issue 1
DOI: 10.1146/annurev.biophys.37.032807.125804

X-ray Structure of the FimC-FimH Chaperone-Adhesin Complex from Uropathogenic Escherichia coli
journal, August 1999

Choudhury, D.
Science, Vol. 285, Issue 5430
DOI: 10.1126/science.285.5430.1061

Assessment of phase accuracy by cross validation: the free R value. Methods and applications
journal, January 1993

Brünger, A. T.
Acta Crystallographica Section D Biological Crystallography, Vol. 49, Issue 1
DOI: 10.1107/S0907444992007352

Transmission of allostery through the lectin domain in selectin-mediated cell adhesion
journal, January 2009

Waldron, Travis T.; Springer, Timothy A.
Proceedings of the National Academy of Sciences, Vol. 106, Issue 1
DOI: 10.1073/pnas.0810620105

A deliberate approach to screening for initial crystallization conditions of biological macromolecules
journal, April 2003

Luft, Joseph R.; Collins, Robert J.; Fehrman, Nancy A.
Journal of Structural Biology, Vol. 142, Issue 1
DOI: 10.1016/S1047-8477(03)00048-0

[20] Processing of X-ray diffraction data collected in oscillation mode
book, January 1997

Otwinowski, Zbyszek; Minor, Wladek
Macromolecular Crystallography Part A, 307-326
DOI: 10.1016/S0076-6879(97)76066-X

VMD: Visual molecular dynamics
journal, February 1996

Humphrey, William; Dalke, Andrew; Schulten, Klaus
Journal of Molecular Graphics, Vol. 14, Issue 1
DOI: 10.1016/0263-7855(96)00018-5

Phaser crystallographic software
journal, July 2007

McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
Journal of Applied Crystallography, Vol. 40, Issue 4
DOI: 10.1107/S0021889807021206

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Title: Structural Basis for Mechanical Force Regulation of the Adhesin FimH via Finger Trap-like β Sheet Twisting

Citation Formats

Measuring Receptor/Ligand Interaction at the Single-Bond Level: Experimental and Interpretative Issues journal, March 2002

MODIP revisited: re-evaluation and refinement of an automated procedure for modeling of disulfide bonds in proteins journal, March 2003

Integrin-like Allosteric Properties of the Catch Bond-forming FimH Adhesin of Escherichia coli journal, March 2008

Refinement of Macromolecular Structures by the Maximum-Likelihood Method journal, May 1997

Shear-dependent ‘stick-and-roll’ adhesion of type 1 fimbriated Escherichia coli: Stick-and-roll bacterial adhesion journal, August 2004

Bacterial Adhesion to Target Cells Enhanced by Shear Force journal, June 2002

Rapid automated molecular replacement by evolutionary search journal, February 1999

Intervening with Urinary Tract Infections Using Anti-Adhesives Based on the Crystal Structure of the FimH–Oligomannose-3 Complex journal, April 2008

NMR Structure of the Escherichia coli Type 1 Pilus Subunit FimF and Its Interactions with Other Pilus Subunits journal, January 2008

Catch-Bond Model Derived from Allostery Explains Force-Activated Bacterial Adhesion journal, February 2006

Structural basis of tropism of Escherichia coli to the bladder during urinary tract infection: FimH mannose-binding pocket journal, May 2002

Importance of Force Linkage in Mechanochemistry of Adhesion Receptors journal, November 2006

Type II Cadherin Ectodomain Structures: Implications for Classical Cadherin Specificity journal, March 2006

Interdomain Interaction in the FimH Adhesin of Escherichia coli Regulates the Affinity to Mannose journal, June 2007

The structure of the rigor complex and its implications for the power stroke journal, December 2004

Receptor binding studies disclose a novel class of high-affinity inhibitors of the Escherichia coli FimH adhesin journal, November 2004

The CCP4 suite programs for protein crystallography journal, September 1994

Improved Fourier coefficients for maps using phases from partial structures with errors journal, May 1986

Mechanically Activated Integrin Switch Controls α 5 β 1 Function journal, January 2009

Linking integrin conformation to function journal, January 2009

FimH Forms Catch Bonds That Are Enhanced by Mechanical Force Due to Allosteric Regulation journal, April 2008

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Autoinhibitory Domains: Modular Effectors of Cellular Regulation journal, November 2002

Infinite Kinetic Stability against Dissociation of Supramolecular Protein Complexes through Donor Strand Complementation journal, April 2008

Selection Footprint in the FimH Adhesin Shows Pathoadaptive Niche Differentiation in Escherichia coli journal, March 2004

Structural basis for allostery in integrins and binding to fibrinogen-mimetic therapeutics journal, September 2004

Integrin Structure, Allostery, and Bidirectional Signaling journal, November 2005

Weak Rolling Adhesion Enhances Bacterial Surface Colonization journal, March 2007

Force as a Facilitator of Integrin Conformational Changes during Leukocyte Arrest on Blood Vessels and Antigen-Presenting Cells journal, January 2007

Coot model-building tools for molecular graphics journal, November 2004

Specificity of cell-cell adhesion by classical cadherins: Critical role for low-affinity dimerization through -strand swapping journal, June 2005

The adhesive binding site of cadherins revisited journal, December 1999

Structure and function of laminin LG modules journal, August 2000

Structural basis for selectin mechanochemistry journal, December 2008

Remodeling of the lectin–EGF-like domain interface in P- and L-selectin increases adhesiveness and shear resistance under hydrodynamic force journal, July 2006

SURVEY AND SUMMARY: The morph server: a standardized system for analyzing and visualizing macromolecular motions in a database framework journal, April 2000

XtalView/Xfit—A Versatile Program for Manipulating Atomic Coordinates and Electron Density journal, April 1999

Crystal structure of the ternary FimC-FimF t -FimD N complex indicates conserved pilus chaperone-subunit complex recognition by the usher FimD journal, January 2008

NMRPipe: A multidimensional spectral processing system based on UNIX pipes journal, November 1995

Elevated Shear Stress Protects Escherichia coli Cells Adhering to Surfaces via Catch Bonds from Detachment by Soluble Inhibitors journal, April 2006

Recent structural studies of carbohydrate-binding modules journal, November 2006

Structural plasticity in Ig superfamily domain 4 of ICAM-1 mediates cell surface dimerization journal, September 2007

MOLREP an Automated Program for Molecular Replacement journal, December 1997

Biophysics of Catch Bonds journal, June 2008

X-ray Structure of the FimC-FimH Chaperone-Adhesin Complex from Uropathogenic Escherichia coli journal, August 1999

Assessment of phase accuracy by cross validation: the free R value. Methods and applications journal, January 1993

Transmission of allostery through the lectin domain in selectin-mediated cell adhesion journal, January 2009

A deliberate approach to screening for initial crystallization conditions of biological macromolecules journal, April 2003

[20] Processing of X-ray diffraction data collected in oscillation mode book, January 1997

VMD: Visual molecular dynamics journal, February 1996

Phaser crystallographic software journal, July 2007

Measuring Receptor/Ligand Interaction at the Single-Bond Level: Experimental and Interpretative Issues
journal, March 2002

MODIP revisited: re-evaluation and refinement of an automated procedure for modeling of disulfide bonds in proteins
journal, March 2003

Integrin-like Allosteric Properties of the Catch Bond-forming FimH Adhesin of Escherichia coli
journal, March 2008

Refinement of Macromolecular Structures by the Maximum-Likelihood Method
journal, May 1997

Shear-dependent ‘stick-and-roll’ adhesion of type 1 fimbriated Escherichia coli: Stick-and-roll bacterial adhesion
journal, August 2004

Bacterial Adhesion to Target Cells Enhanced by Shear Force
journal, June 2002

Rapid automated molecular replacement by evolutionary search
journal, February 1999

Intervening with Urinary Tract Infections Using Anti-Adhesives Based on the Crystal Structure of the FimH–Oligomannose-3 Complex
journal, April 2008

NMR Structure of the Escherichia coli Type 1 Pilus Subunit FimF and Its Interactions with Other Pilus Subunits
journal, January 2008

Catch-Bond Model Derived from Allostery Explains Force-Activated Bacterial Adhesion
journal, February 2006

Structural basis of tropism of Escherichia coli to the bladder during urinary tract infection: FimH mannose-binding pocket
journal, May 2002

Importance of Force Linkage in Mechanochemistry of Adhesion Receptors
journal, November 2006

Type II Cadherin Ectodomain Structures: Implications for Classical Cadherin Specificity
journal, March 2006

Interdomain Interaction in the FimH Adhesin of Escherichia coli Regulates the Affinity to Mannose
journal, June 2007

The structure of the rigor complex and its implications for the power stroke
journal, December 2004

Receptor binding studies disclose a novel class of high-affinity inhibitors of the Escherichia coli FimH adhesin
journal, November 2004

The CCP4 suite programs for protein crystallography
journal, September 1994

Improved Fourier coefficients for maps using phases from partial structures with errors
journal, May 1986

Mechanically Activated Integrin Switch Controls α ₅ β ₁ Function
journal, January 2009

Linking integrin conformation to function
journal, January 2009

FimH Forms Catch Bonds That Are Enhanced by Mechanical Force Due to Allosteric Regulation
journal, April 2008

Crystal structures of fibronectin-binding sites from Staphylococcus aureus FnBPA in complex with fibronectin domains
journal, August 2008

Autoinhibitory Domains: Modular Effectors of Cellular Regulation
journal, November 2002

Infinite Kinetic Stability against Dissociation of Supramolecular Protein Complexes through Donor Strand Complementation
journal, April 2008

Selection Footprint in the FimH Adhesin Shows Pathoadaptive Niche Differentiation in Escherichia coli
journal, March 2004

Structural basis for allostery in integrins and binding to fibrinogen-mimetic therapeutics
journal, September 2004

Integrin Structure, Allostery, and Bidirectional Signaling
journal, November 2005

Weak Rolling Adhesion Enhances Bacterial Surface Colonization
journal, March 2007

Force as a Facilitator of Integrin Conformational Changes during Leukocyte Arrest on Blood Vessels and Antigen-Presenting Cells
journal, January 2007

Coot model-building tools for molecular graphics
journal, November 2004

Specificity of cell-cell adhesion by classical cadherins: Critical role for low-affinity dimerization through -strand swapping
journal, June 2005

The adhesive binding site of cadherins revisited
journal, December 1999

Structure and function of laminin LG modules
journal, August 2000

Structural basis for selectin mechanochemistry
journal, December 2008

Remodeling of the lectin–EGF-like domain interface in P- and L-selectin increases adhesiveness and shear resistance under hydrodynamic force
journal, July 2006

SURVEY AND SUMMARY: The morph server: a standardized system for analyzing and visualizing macromolecular motions in a database framework
journal, April 2000

XtalView/Xfit—A Versatile Program for Manipulating Atomic Coordinates and Electron Density
journal, April 1999

Crystal structure of the ternary FimC-FimF _t -FimD _N complex indicates conserved pilus chaperone-subunit complex recognition by the usher FimD
journal, January 2008

NMRPipe: A multidimensional spectral processing system based on UNIX pipes
journal, November 1995

Elevated Shear Stress Protects Escherichia coli Cells Adhering to Surfaces via Catch Bonds from Detachment by Soluble Inhibitors
journal, April 2006

Recent structural studies of carbohydrate-binding modules
journal, November 2006

Structural plasticity in Ig superfamily domain 4 of ICAM-1 mediates cell surface dimerization
journal, September 2007

MOLREP an Automated Program for Molecular Replacement
journal, December 1997

Biophysics of Catch Bonds
journal, June 2008

X-ray Structure of the FimC-FimH Chaperone-Adhesin Complex from Uropathogenic Escherichia coli
journal, August 1999

Assessment of phase accuracy by cross validation: the free R value. Methods and applications
journal, January 1993

Transmission of allostery through the lectin domain in selectin-mediated cell adhesion
journal, January 2009

A deliberate approach to screening for initial crystallization conditions of biological macromolecules
journal, April 2003

[20] Processing of X-ray diffraction data collected in oscillation mode
book, January 1997

VMD: Visual molecular dynamics
journal, February 1996

Phaser crystallographic software
journal, July 2007