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Title: Analysis of Casein Biopolymers Adsorption to Lignocellulosic Biomass as a Potential Cellulase Stabilizer

Abstract

Although lignocellulosic materials have a good potential to substitute current feedstocks used for ethanol production, conversion of these materials to fermentable sugars is still not economical through enzymatic hydrolysis. High cost of cellulase has prompted research to explore techniques that can prevent from enzyme deactivation. Colloidal proteins of casein can form monolayers on hydrophobic surfaces that alleviate the de-activation of protein of interest. Scanning electron microscope (SEM), fourier transform infrared spectroscopy (FT-IR), capillary electrophoresis (CE), and Kjeldahl and BSA protein assays were used to investigate the unknown mechanism of action of induced cellulase activity during hydrolysis of casein-treated biomass. Adsorption of casein to biomass was observed with all of the analytical techniques used and varied depending on the pretreatment techniques of biomass. FT-IR analysis of amides I and II suggested that the substructure of protein from casein or skim milk were deformed at the time of contact with biomass. With no additive, the majority of one of the cellulase mono-component, 97.1 ± 1.1, was adsorbed to CS within 24 h, this adsorption was irreversible and increased by 2% after 72 h. However, biomass treatment with skim-milk and casein reduced the adsorption to 32.9% ± 6.0 and 82.8% ± 6.0, respectively.

Authors:
 [1];  [1];  [2]
  1. Department of Agricultural and Biosystems Engineering, South Dakota State University, 1400 North Campus Drive, Brookings, SD 57007, USA
  2. Department of Biology and Microbiology, South Dakota State University, 1400 North Campus Drive, Brookings, SD 57007, USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1198032
Grant/Contract Number:  
FG36-08GO8807
Resource Type:
Published Article
Journal Name:
Journal of Biomedicine and Biotechnology
Additional Journal Information:
Journal Name: Journal of Biomedicine and Biotechnology Journal Volume: 2012; Journal ID: ISSN 1110-7243
Publisher:
Hindawi Publishing Corporation
Country of Publication:
Country unknown/Code not available
Language:
English

Citation Formats

Eckard, Anahita Dehkhoda, Muthukumarappan, Kasiviswanathan, and Gibbons, William. Analysis of Casein Biopolymers Adsorption to Lignocellulosic Biomass as a Potential Cellulase Stabilizer. Country unknown/Code not available: N. p., 2012. Web. https://doi.org/10.1155/2012/745181.
Eckard, Anahita Dehkhoda, Muthukumarappan, Kasiviswanathan, & Gibbons, William. Analysis of Casein Biopolymers Adsorption to Lignocellulosic Biomass as a Potential Cellulase Stabilizer. Country unknown/Code not available. https://doi.org/10.1155/2012/745181
Eckard, Anahita Dehkhoda, Muthukumarappan, Kasiviswanathan, and Gibbons, William. Sun . "Analysis of Casein Biopolymers Adsorption to Lignocellulosic Biomass as a Potential Cellulase Stabilizer". Country unknown/Code not available. https://doi.org/10.1155/2012/745181.
@article{osti_1198032,
title = {Analysis of Casein Biopolymers Adsorption to Lignocellulosic Biomass as a Potential Cellulase Stabilizer},
author = {Eckard, Anahita Dehkhoda and Muthukumarappan, Kasiviswanathan and Gibbons, William},
abstractNote = {Although lignocellulosic materials have a good potential to substitute current feedstocks used for ethanol production, conversion of these materials to fermentable sugars is still not economical through enzymatic hydrolysis. High cost of cellulase has prompted research to explore techniques that can prevent from enzyme deactivation. Colloidal proteins of casein can form monolayers on hydrophobic surfaces that alleviate the de-activation of protein of interest. Scanning electron microscope (SEM), fourier transform infrared spectroscopy (FT-IR), capillary electrophoresis (CE), and Kjeldahl and BSA protein assays were used to investigate the unknown mechanism of action of induced cellulase activity during hydrolysis of casein-treated biomass. Adsorption of casein to biomass was observed with all of the analytical techniques used and varied depending on the pretreatment techniques of biomass. FT-IR analysis of amides I and II suggested that the substructure of protein from casein or skim milk were deformed at the time of contact with biomass. With no additive, the majority of one of the cellulase mono-component, 97.1 ± 1.1, was adsorbed to CS within 24 h, this adsorption was irreversible and increased by 2% after 72 h. However, biomass treatment with skim-milk and casein reduced the adsorption to 32.9% ± 6.0 and 82.8% ± 6.0, respectively.},
doi = {10.1155/2012/745181},
journal = {Journal of Biomedicine and Biotechnology},
number = ,
volume = 2012,
place = {Country unknown/Code not available},
year = {2012},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1155/2012/745181

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