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Title: Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling

Abstract

Comprehensive detection and quantitation of metabolites from a biological source constitute the major challenges of current metabolomics research. Two chemical derivatization methodologies, butylation and amination, were applied to human serum for ionization enhancement of a broad spectrum of metabolite classes, including steroids and amino acids. LC-ESI-MS analysis of the derivatized serum samples provided a significant signal elevation across the total ion chromatogram to over a 100-fold increase in ionization efficiency. It was also demonstrated that derivatization combined with isotopically labeled reagents facilitated the relative quantitation of derivatized metabolites from individual as well as pooled samples.

Authors:
 [1];  [1];  [1];  [1];  [1];  [2];  [1];  [1];  [1];  [1];  [1];  [3];  [3];  [1]
  1. The Center for Mass Spectrometry and Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA-92037, USA
  2. Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, CA-92037, USA
  3. Becton Dickinson, Sparks, MD-21152, USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1198015
Grant/Contract Number:  
AC02-05CH1123
Resource Type:
Published Article
Journal Name:
Spectroscopy
Additional Journal Information:
Journal Name: Spectroscopy Journal Volume: 22 Journal Issue: 5; Journal ID: ISSN 0712-4813
Publisher:
Hindawi Publishing Corporation
Country of Publication:
Country unknown/Code not available
Language:
English

Citation Formats

O'Maille, Grace, Go, Eden P., Hoang, Linh, Want, Elizabeth J., Smith, Colin, O'Maille, Paul, NordstrÖm, Anders, Morita, Hirotoshi, Qin, Chuan, Uritboonthai, Wilasinee, Apon, Junefredo, Moore, Richard, Garrett, James, and Siuzdak, Gary. Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling. Country unknown/Code not available: N. p., 2008. Web. doi:10.1155/2008/715905.
O'Maille, Grace, Go, Eden P., Hoang, Linh, Want, Elizabeth J., Smith, Colin, O'Maille, Paul, NordstrÖm, Anders, Morita, Hirotoshi, Qin, Chuan, Uritboonthai, Wilasinee, Apon, Junefredo, Moore, Richard, Garrett, James, & Siuzdak, Gary. Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling. Country unknown/Code not available. doi:10.1155/2008/715905.
O'Maille, Grace, Go, Eden P., Hoang, Linh, Want, Elizabeth J., Smith, Colin, O'Maille, Paul, NordstrÖm, Anders, Morita, Hirotoshi, Qin, Chuan, Uritboonthai, Wilasinee, Apon, Junefredo, Moore, Richard, Garrett, James, and Siuzdak, Gary. Tue . "Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling". Country unknown/Code not available. doi:10.1155/2008/715905.
@article{osti_1198015,
title = {Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling},
author = {O'Maille, Grace and Go, Eden P. and Hoang, Linh and Want, Elizabeth J. and Smith, Colin and O'Maille, Paul and NordstrÖm, Anders and Morita, Hirotoshi and Qin, Chuan and Uritboonthai, Wilasinee and Apon, Junefredo and Moore, Richard and Garrett, James and Siuzdak, Gary},
abstractNote = {Comprehensive detection and quantitation of metabolites from a biological source constitute the major challenges of current metabolomics research. Two chemical derivatization methodologies, butylation and amination, were applied to human serum for ionization enhancement of a broad spectrum of metabolite classes, including steroids and amino acids. LC-ESI-MS analysis of the derivatized serum samples provided a significant signal elevation across the total ion chromatogram to over a 100-fold increase in ionization efficiency. It was also demonstrated that derivatization combined with isotopically labeled reagents facilitated the relative quantitation of derivatized metabolites from individual as well as pooled samples.},
doi = {10.1155/2008/715905},
journal = {Spectroscopy},
number = 5,
volume = 22,
place = {Country unknown/Code not available},
year = {2008},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1155/2008/715905

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