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Title: Genetic Confirmation of the Role of Sulfopyruvate Decarboxylase in Coenzyme M Biosynthesis in Methanococcus maripaludis

Abstract

Coenzyme M is an essential coenzyme for methanogenesis. The proposed biosynthetic pathway consists of five steps, of which the fourth step is catalyzed by sulfopyruvate decarboxylase (ComDE). Disruption of the gene comE by transposon mutagenesis resulted in a partial coenzyme M auxotroph, which grew poorly in the absence of coenzyme M and retained less than 3% of the wild type level of coenzyme M biosynthesis. Upon coenzyme M addition, normal growth of the mutant was restored. Moreover, complementation of the mutation with the wild type comE gene in trans restored full growth in the absence of coenzyme M. These results confirm that ComE plays an important role in coenzyme M biosynthesis. The inability to yield a complete CoM auxotroph suggests that either the transposon insertion failed to completely inactivate the gene or M. maripaludis possesses a promiscuous activity that partially complemented the mutation.

Authors:
 [1];  [1];  [1]
  1. Department of Microbiology, University of Georgia, 541 Biological Science Building, Athens, GA 30602-2605, USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1197873
Resource Type:
Published Article
Journal Name:
Archaea
Additional Journal Information:
Journal Name: Archaea Journal Volume: 2013; Journal ID: ISSN 1472-3646
Publisher:
Hindawi Publishing Corporation
Country of Publication:
United States
Language:
English

Citation Formats

Sarmiento, Felipe, Ellison, Courtney K., and Whitman, William B. Genetic Confirmation of the Role of Sulfopyruvate Decarboxylase in Coenzyme M Biosynthesis in Methanococcus maripaludis. United States: N. p., 2013. Web. doi:10.1155/2013/185250.
Sarmiento, Felipe, Ellison, Courtney K., & Whitman, William B. Genetic Confirmation of the Role of Sulfopyruvate Decarboxylase in Coenzyme M Biosynthesis in Methanococcus maripaludis. United States. doi:10.1155/2013/185250.
Sarmiento, Felipe, Ellison, Courtney K., and Whitman, William B. Tue . "Genetic Confirmation of the Role of Sulfopyruvate Decarboxylase in Coenzyme M Biosynthesis in Methanococcus maripaludis". United States. doi:10.1155/2013/185250.
@article{osti_1197873,
title = {Genetic Confirmation of the Role of Sulfopyruvate Decarboxylase in Coenzyme M Biosynthesis in Methanococcus maripaludis},
author = {Sarmiento, Felipe and Ellison, Courtney K. and Whitman, William B.},
abstractNote = {Coenzyme M is an essential coenzyme for methanogenesis. The proposed biosynthetic pathway consists of five steps, of which the fourth step is catalyzed by sulfopyruvate decarboxylase (ComDE). Disruption of the gene comE by transposon mutagenesis resulted in a partial coenzyme M auxotroph, which grew poorly in the absence of coenzyme M and retained less than 3% of the wild type level of coenzyme M biosynthesis. Upon coenzyme M addition, normal growth of the mutant was restored. Moreover, complementation of the mutation with the wild type comE gene in trans restored full growth in the absence of coenzyme M. These results confirm that ComE plays an important role in coenzyme M biosynthesis. The inability to yield a complete CoM auxotroph suggests that either the transposon insertion failed to completely inactivate the gene or M. maripaludis possesses a promiscuous activity that partially complemented the mutation.},
doi = {10.1155/2013/185250},
journal = {Archaea},
number = ,
volume = 2013,
place = {United States},
year = {2013},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1155/2013/185250

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