FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis
Abstract
Abscisic acid (ABA) is a plant hormone that regulates plant growth and development and mediates abiotic stress responses. Direct cellular monitoring of dynamic ABA concentration changes in response to environmental cues is essential for understanding ABA action. We have developed ABAleons: ABA-specific optogenetic reporters that instantaneously convert the phytohormone-triggered interaction of ABA receptors with PP2C-type phosphatases to send a fluorescence resonance energy transfer (FRET) signal in response to ABA. We report the design, engineering and use of ABAleons with ABA affinities in the range of 100–600 nM to map ABA concentration changes in plant tissues with spatial and temporal resolution. High ABAleon expression can partially repress Arabidopsis ABA responses. ABAleons report ABA concentration differences in distinct cell types, ABA concentration increases in response to low humidity and NaCl in guard cells and to NaCl and osmotic stress in roots and ABA transport from the hypocotyl to the shoot and root.
- Authors:
- Publication Date:
- Research Org.:
- Univ. of California, San Diego, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
- OSTI Identifier:
- 1197664
- Alternate Identifier(s):
- OSTI ID: 1197665; OSTI ID: 1628805
- Grant/Contract Number:
- FG02-03ER15449
- Resource Type:
- Published Article
- Journal Name:
- eLife
- Additional Journal Information:
- Journal Name: eLife Journal Volume: 3; Journal ID: ISSN 2050-084X
- Publisher:
- eLife Sciences Publications, Ltd.
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Life Sciences & Biomedicine - Other Topics
Citation Formats
Waadt, Rainer, Hitomi, Kenichi, Nishimura, Noriyuki, Hitomi, Chiharu, Adams, Stephen R., Getzoff, Elizabeth D., and Schroeder, Julian I. FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis. United States: N. p., 2014.
Web. doi:10.7554/eLife.01739.
Waadt, Rainer, Hitomi, Kenichi, Nishimura, Noriyuki, Hitomi, Chiharu, Adams, Stephen R., Getzoff, Elizabeth D., & Schroeder, Julian I. FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis. United States. https://doi.org/10.7554/eLife.01739
Waadt, Rainer, Hitomi, Kenichi, Nishimura, Noriyuki, Hitomi, Chiharu, Adams, Stephen R., Getzoff, Elizabeth D., and Schroeder, Julian I. Tue .
"FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis". United States. https://doi.org/10.7554/eLife.01739.
@article{osti_1197664,
title = {FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis},
author = {Waadt, Rainer and Hitomi, Kenichi and Nishimura, Noriyuki and Hitomi, Chiharu and Adams, Stephen R. and Getzoff, Elizabeth D. and Schroeder, Julian I.},
abstractNote = {Abscisic acid (ABA) is a plant hormone that regulates plant growth and development and mediates abiotic stress responses. Direct cellular monitoring of dynamic ABA concentration changes in response to environmental cues is essential for understanding ABA action. We have developed ABAleons: ABA-specific optogenetic reporters that instantaneously convert the phytohormone-triggered interaction of ABA receptors with PP2C-type phosphatases to send a fluorescence resonance energy transfer (FRET) signal in response to ABA. We report the design, engineering and use of ABAleons with ABA affinities in the range of 100–600 nM to map ABA concentration changes in plant tissues with spatial and temporal resolution. High ABAleon expression can partially repress Arabidopsis ABA responses. ABAleons report ABA concentration differences in distinct cell types, ABA concentration increases in response to low humidity and NaCl in guard cells and to NaCl and osmotic stress in roots and ABA transport from the hypocotyl to the shoot and root.},
doi = {10.7554/eLife.01739},
journal = {eLife},
number = ,
volume = 3,
place = {United States},
year = {Tue Apr 15 00:00:00 EDT 2014},
month = {Tue Apr 15 00:00:00 EDT 2014}
}
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