skip to main content

DOE PAGESDOE PAGES

Title: Comparison of Two Site-Specifically 18F-Labeled Affibodies for PET Imaging of EGFR Positive Tumors

The epidermal growth factor receptor (EGFR) serves as an attractive target for cancer molecular imaging and therapy. Our previous positron emission tomography (PET) studies showed that the EGFR-targeting affibody molecules 64Cu-DOTA-Z EGFR:1907 and 18F-FBEM-Z EGFR:1907 can discriminate between high and low EGFR-expression tumors and have the potential for patient selection for EGFR-targeted therapy. Compared with 64Cu, 18F may improve imaging of EGFR-expression and is more suitable for clinical application, but the labeling reaction of 18F-FBEM-Z EGFR:1907 requires a long synthesis time. The aim of the present study is to develop a new generation of 18F labeled affibody probes (Al 18F-NOTA-Z EGFR:1907 and 18F-CBT-Z EGFR:1907) and to determine whether they are suitable agents for imaging of EGFR expression. The first approach consisted of conjugating Z EGFR:1907 with NOTA and radiolabeling with Al 18F to produce Al 18F-NOTA-Z EGFR:1907. In a second approach the prosthetic group 18F-labeled-2-cyanobenzothiazole ( 18F-CBT) was conjugated to Cys-Z EGFR:1907 to produce 18F-CBT-Z EGFR:1907. Binding affinity and specificity of Al 18F-NOTA-Z EGFR:1907 and 18F-CBT-Z EGFR:1907 to EGFR were evaluated using A431 cells. Biodistribution and PET studies were conducted on mice bearing A431 xenografts after injection of Al 18F-NOTA-Z EGFR:1907 or 18F-CBT-Z EGFR:1907 with or without coinjection of unlabeledmore » affibody proteins. The radiosyntheses of Al 18F-NOTA-Z EGFR:1907 and 18F-CBT-Z EGFR:1907 were completed successfully within 40 and 120 min with a decay-corrected yield of 15% and 41% using a 2-step, 1-pot reaction and 2-step, 2-pot reaction, respectively. Both probes bound to EGFR with low nanomolar affinity in A431 cells. Although 18F-CBT-Z EGFR:1907 showed instability in vivo, biodistribution studies revealed rapid and high tumor accumulation and quick clearance from normal tissues except the bones. In contrast, Al 18F-NOTA-Z EGFR:1907 demonstrated high in vitro and in vivo stability, high tumor uptake, and relative low uptake in most of the normal organs except the liver and kidneys at 3 h after injection. The specificity of both probes for A431 tumors was confirmed by their lower uptake on coinjection of unlabeled affibody. PET studies showed that Al 18F-NOTA-Z EGFR:1907 and 18F-CBT-Z EGFR:1907 could clearly identify EGFR positive tumors with good contrast. Two strategies for 18F-labeling of affibody molecules were successfully developed as two model platforms using NOTA or CBT coupling to affibody molecules that contain an N-terminal cysteine. Al 18F-NOTA-Z EGFR:1907 and 18F-CBT-Z EGFR:1907 can be reliably obtained in a relatively short time. Biodistribution and PET studies demonstrated that Al 18F-NOTA-Z EGFR:1907 is a promising PET probe for imaging EGFR expression in living mice.« less
Authors:
 [1] ;  [2] ;  [3] ;  [2] ;  [4] ;  [2] ;  [2] ;  [2] ;  [5] ;  [2]
  1. Zhongshan Hospital Xiamen University, Xiamen (China). Department of Nuclear Medicine; Stanford University School of Medicine, CA (United States). Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Bio-X Program, and Stanford Cancer Center
  2. Stanford University School of Medicine, CA (United States). Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Bio-X Program, and Stanford Cancer Center
  3. Stanford University School of Medicine, CA (United States). Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Bio-X Program, and Stanford Cancer Center; Korea Atomic Energy Research Institute (Republic of Korea). Research Division for Biotechnology, Advanced Radiation Technology Institute
  4. Stanford University School of Medicine, CA (United States). Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Bio-X Program, and Stanford Cancer Center; Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre (Brazil). Instituto do Cérebro do Rio Grande do Sul (InsCer)
  5. The First Affiliated Hospital of Xiamen University, Xiamen (China). Department of Nuclear Medicine
Publication Date:
Grant/Contract Number:
SC0008397
Type:
Published Article
Journal Name:
Molecular Pharmaceutics
Additional Journal Information:
Journal Volume: 11; Journal Issue: 11; Journal ID: ISSN 1543-8384
Publisher:
American Chemical Society (ACS)
Research Org:
Stanford Univ., CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 18F; affibody; CBT; EGFR; NOTA; PET
OSTI Identifier:
1188056
Alternate Identifier(s):
OSTI ID: 1440565

Su, Xinhui, Cheng, Kai, Jeon, Jongho, Shen, Bin, Venturin, Gianina Teribele, Hu, Xiang, Rao, Jianghong, Chin, Frederick T., Wu, Hua, and Cheng, Zhen. Comparison of Two Site-Specifically 18F-Labeled Affibodies for PET Imaging of EGFR Positive Tumors. United States: N. p., Web. doi:10.1021/mp5003043.
Su, Xinhui, Cheng, Kai, Jeon, Jongho, Shen, Bin, Venturin, Gianina Teribele, Hu, Xiang, Rao, Jianghong, Chin, Frederick T., Wu, Hua, & Cheng, Zhen. Comparison of Two Site-Specifically 18F-Labeled Affibodies for PET Imaging of EGFR Positive Tumors. United States. doi:10.1021/mp5003043.
Su, Xinhui, Cheng, Kai, Jeon, Jongho, Shen, Bin, Venturin, Gianina Teribele, Hu, Xiang, Rao, Jianghong, Chin, Frederick T., Wu, Hua, and Cheng, Zhen. 2014. "Comparison of Two Site-Specifically 18F-Labeled Affibodies for PET Imaging of EGFR Positive Tumors". United States. doi:10.1021/mp5003043.
@article{osti_1188056,
title = {Comparison of Two Site-Specifically 18F-Labeled Affibodies for PET Imaging of EGFR Positive Tumors},
author = {Su, Xinhui and Cheng, Kai and Jeon, Jongho and Shen, Bin and Venturin, Gianina Teribele and Hu, Xiang and Rao, Jianghong and Chin, Frederick T. and Wu, Hua and Cheng, Zhen},
abstractNote = {The epidermal growth factor receptor (EGFR) serves as an attractive target for cancer molecular imaging and therapy. Our previous positron emission tomography (PET) studies showed that the EGFR-targeting affibody molecules 64Cu-DOTA-ZEGFR:1907 and 18F-FBEM-ZEGFR:1907 can discriminate between high and low EGFR-expression tumors and have the potential for patient selection for EGFR-targeted therapy. Compared with 64Cu, 18F may improve imaging of EGFR-expression and is more suitable for clinical application, but the labeling reaction of 18F-FBEM-ZEGFR:1907 requires a long synthesis time. The aim of the present study is to develop a new generation of 18F labeled affibody probes (Al18F-NOTA-ZEGFR:1907 and 18F-CBT-ZEGFR:1907) and to determine whether they are suitable agents for imaging of EGFR expression. The first approach consisted of conjugating ZEGFR:1907 with NOTA and radiolabeling with Al18F to produce Al18F-NOTA-ZEGFR:1907. In a second approach the prosthetic group 18F-labeled-2-cyanobenzothiazole (18F-CBT) was conjugated to Cys-ZEGFR:1907 to produce 18F-CBT-ZEGFR:1907. Binding affinity and specificity of Al18F-NOTA-ZEGFR:1907 and 18F-CBT-ZEGFR:1907 to EGFR were evaluated using A431 cells. Biodistribution and PET studies were conducted on mice bearing A431 xenografts after injection of Al18F-NOTA-ZEGFR:1907 or 18F-CBT-ZEGFR:1907 with or without coinjection of unlabeled affibody proteins. The radiosyntheses of Al18F-NOTA-ZEGFR:1907 and 18F-CBT-ZEGFR:1907 were completed successfully within 40 and 120 min with a decay-corrected yield of 15% and 41% using a 2-step, 1-pot reaction and 2-step, 2-pot reaction, respectively. Both probes bound to EGFR with low nanomolar affinity in A431 cells. Although 18F-CBT-ZEGFR:1907 showed instability in vivo, biodistribution studies revealed rapid and high tumor accumulation and quick clearance from normal tissues except the bones. In contrast, Al18F-NOTA-ZEGFR:1907 demonstrated high in vitro and in vivo stability, high tumor uptake, and relative low uptake in most of the normal organs except the liver and kidneys at 3 h after injection. The specificity of both probes for A431 tumors was confirmed by their lower uptake on coinjection of unlabeled affibody. PET studies showed that Al18F-NOTA-ZEGFR:1907 and 18F-CBT-ZEGFR:1907 could clearly identify EGFR positive tumors with good contrast. Two strategies for 18F-labeling of affibody molecules were successfully developed as two model platforms using NOTA or CBT coupling to affibody molecules that contain an N-terminal cysteine. Al18F-NOTA-ZEGFR:1907 and 18F-CBT-ZEGFR:1907 can be reliably obtained in a relatively short time. Biodistribution and PET studies demonstrated that Al18F-NOTA-ZEGFR:1907 is a promising PET probe for imaging EGFR expression in living mice.},
doi = {10.1021/mp5003043},
journal = {Molecular Pharmaceutics},
number = 11,
volume = 11,
place = {United States},
year = {2014},
month = {6}
}