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Title: Newly identified helper bacteria stimulate ectomycorrhizal formation in Populus

Abstract

Mycorrhiza helper bacteria (MHB) are known to increase host root colonization by mycorrhizal fungi but the molecular mechanisms and potential tripartite trophic interactions are poorly understood. Through an effort to study Populus microbiome, we isolated 21 Pseudomonas strains from native Populus deltoides roots. These bacterial isolates were characterized and screened for MHB effectiveness on the Populus-Laccaria system. Two other Pseudomonas strains (i.e., Pf-5 and BBc6R8) from existing collections were also included as reference in the screening process. We analyzed Laccaria bicolor S238N growth rate, mycelial architecture and transcriptional changes induced by the contrasting Pseudomonas strains (i.e., inhibitory, neutral and beneficial). We characterized 17 out of the 21 Pseudomonas strains from the Populus rhizosphere with positive effects on L. bicolor S238N growth, as well as on Populus root architecture and colonization by L. bicolor S238N across three Populus species. Four of seven reporter genes, Tra1, Tectonin2, Gcn5 and Cipc1, thought to be specific to the interaction with strain BBc6R8, were induced or repressed while interacting with six (i.e., GM17, GM33, GM41, GM48, Pf-5 and BBc6R8) of the tested Pseudomonas strains. GM41 promoted the highest roots colonization across three Populus species but most notably in P. deltoides, which is otherwise, poorly colonizedmore » by L. bicolor. Here we report novel MHB strains isolated from native Populus that improve roots colonization. This tripartite relationship could be exploited in nursery production for target Populus species/genotypes as a means of improving establishment and survival in marginal lands.« less

Authors:
 [1];  [1];  [1];  [1];  [1]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Div.
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1185358
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
Journal Volume: 5; Journal Issue: 579; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Mycorrhiza helper bacteria; mycorrhizal fungi; Laccaria bicolor; mutualistic interactions; fungal-bacterial-Populus interactions; Populus rhizosphere; Soil Microbiology

Citation Formats

Labbe, Jessy L., Weston, David J., Dunkirk, Nora, Pelletier, Dale A., and Tuskan, Gerald A. Newly identified helper bacteria stimulate ectomycorrhizal formation in Populus. United States: N. p., 2014. Web. doi:10.3389/fpls.2014.00579.
Labbe, Jessy L., Weston, David J., Dunkirk, Nora, Pelletier, Dale A., & Tuskan, Gerald A. Newly identified helper bacteria stimulate ectomycorrhizal formation in Populus. United States. doi:10.3389/fpls.2014.00579.
Labbe, Jessy L., Weston, David J., Dunkirk, Nora, Pelletier, Dale A., and Tuskan, Gerald A. Fri . "Newly identified helper bacteria stimulate ectomycorrhizal formation in Populus". United States. doi:10.3389/fpls.2014.00579. https://www.osti.gov/servlets/purl/1185358.
@article{osti_1185358,
title = {Newly identified helper bacteria stimulate ectomycorrhizal formation in Populus},
author = {Labbe, Jessy L. and Weston, David J. and Dunkirk, Nora and Pelletier, Dale A. and Tuskan, Gerald A.},
abstractNote = {Mycorrhiza helper bacteria (MHB) are known to increase host root colonization by mycorrhizal fungi but the molecular mechanisms and potential tripartite trophic interactions are poorly understood. Through an effort to study Populus microbiome, we isolated 21 Pseudomonas strains from native Populus deltoides roots. These bacterial isolates were characterized and screened for MHB effectiveness on the Populus-Laccaria system. Two other Pseudomonas strains (i.e., Pf-5 and BBc6R8) from existing collections were also included as reference in the screening process. We analyzed Laccaria bicolor S238N growth rate, mycelial architecture and transcriptional changes induced by the contrasting Pseudomonas strains (i.e., inhibitory, neutral and beneficial). We characterized 17 out of the 21 Pseudomonas strains from the Populus rhizosphere with positive effects on L. bicolor S238N growth, as well as on Populus root architecture and colonization by L. bicolor S238N across three Populus species. Four of seven reporter genes, Tra1, Tectonin2, Gcn5 and Cipc1, thought to be specific to the interaction with strain BBc6R8, were induced or repressed while interacting with six (i.e., GM17, GM33, GM41, GM48, Pf-5 and BBc6R8) of the tested Pseudomonas strains. GM41 promoted the highest roots colonization across three Populus species but most notably in P. deltoides, which is otherwise, poorly colonized by L. bicolor. Here we report novel MHB strains isolated from native Populus that improve roots colonization. This tripartite relationship could be exploited in nursery production for target Populus species/genotypes as a means of improving establishment and survival in marginal lands.},
doi = {10.3389/fpls.2014.00579},
journal = {Frontiers in Plant Science},
number = 579,
volume = 5,
place = {United States},
year = {2014},
month = {10}
}

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Cited by: 11 works
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    Works referencing / citing this record:

    The Influence of Microfungi on the Mycelial Growth of Ectomycorrhizal Fungus Tricholoma matsutake
    journal, June 2019


    The Influence of Microfungi on the Mycelial Growth of Ectomycorrhizal Fungus Tricholoma matsutake
    journal, June 2019