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Title: Genome sequencing of the Trichoderma reesei QM9136 mutant identifies a truncation of the transcriptional regulator XYR1 as the cause for its cellulase-negative phenotype

Trichoderma reesei is the main industrial source of cellulases and hemicellulases required for the hydrolysis of biomass to simple sugars, which can then be used in the production of biofuels and biorefineries. The highly productive strains in use today were generated by classical mutagenesis. As byproducts of this procedure, mutants were generated that turned out to be unable to produce cellulases. In order to identify the mutations responsible for this inability, we sequenced the genome of one of these strains, QM9136, and compared it to that of its progenitor T. reesei QM6a.
 [1] ;  [2] ;  [1] ;  [3] ;  [4] ;  [4] ;  [1] ;  [4] ;  [5] ;  [2] ;  [1] ;  [1]
  1. Vienna Univ. of Technology, Vienna (Austria)
  2. IFP Energies Nouvelles, Rueil-Malmaison (France)
  3. Sorbonne Univ., Paris (France)
  4. Joint Genome Inst., Walnut Creek, CA (United States). US Dept. of Energy.
  5. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Report Number(s):
PNNL-SA-110701; LBNL-177776
Journal ID: ISSN 1471-2164; PII: 1526
Grant/Contract Number:
AC02-05CH11231; AC05-76RL01830
Accepted Manuscript
Journal Name:
BMC Genomics
Additional Journal Information:
Journal Volume: 16; Journal Issue: 1; Journal ID: ISSN 1471-2164
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Genomics Division
Sponsoring Org:
USDOE; USDOE Office of Science (SC). Joint Genome Institute; Austrian Science Fund (FWF)
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES; single nucleotide polymorphism; SNP; indel; comparative genomics; classical mutant; XYR1; transcription; factor shuttling; cellulases; Trichoderma reesei; QM9136; transcription factor shuttling
OSTI Identifier:
Alternate Identifier(s):
OSTI ID: 1208746; OSTI ID: 1242698