Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading

Slininger, Patricia J.; Shea-Andersh, Maureen A.; Thompson, Stephanie R.; Dien, Bruce S.; Kurtzman, Cletus P.; Balan, Venkatesh; da Costa Sousa, Leonardo; Uppugundla, Nirmal; Dale, Bruce E; Cotta, Michael A

doi:10.1186/s13068-015-0239-6

Title: Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading

Journal Article · 09 April 2015 · Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-015-0239-6 · OSTI ID:1184797

Slininger, Patricia J. ^[1]; Shea-Andersh, Maureen A. ^[2]; Thompson, Stephanie R. ^[2]; Dien, Bruce S. ^[2]; Kurtzman, Cletus P. ^[3]; Balan, Venkatesh ^[4]; da Costa Sousa, Leonardo ^[4]; Uppugundla, Nirmal ^[4]; Dale, Bruce E ^[4]; Cotta, Michael A ^[2]

USDA National Center for Agricultural Utilization Research, Peoria, IL (United States). Bioenergy Research Unit.; None
USDA National Center for Agricultural Utilization Research, Peoria, IL (United States). Bioenergy Research Unit.
USDA National Center for Agricultural Utilization Research, Peoria, IL (United States). Bacterial Foodborne Pathogens and Mycology Research.
Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center (GLBRC).

Lignocellulosic biomass is an abundant, renewable feedstock useful for the production of fuel-grade ethanol via the processing steps of pretreatment, enzyme hydrolysis, and microbial fermentation. Traditional industrial yeasts do not ferment xylose and are not able to grow, survive, or ferment in concentrated hydrolyzates that contain enough sugar to support economical ethanol recovery since they are laden with toxic byproducts generated during pretreatment. Repetitive culturing in two types of concentrated hydrolyzates was applied along with ethanol challenged xylose-fed continuous culture to force targeted evolution of the native pentose fermenting yeast Scheffersomyces (Pichia) stipitis strain NRRL Y-7124 maintained in the ARS Culture Collection, Peoria, IL. Isolates collected from various enriched populations were screened and ranked based on relative xylose uptake rate and ethanol yield. Ranking on hydrolyzates with and without nutritional supplementation was used to identify those isolates with best performance across diverse conditions. Robust S. stipitis strains adapted to perform very well in enzyme hydrolyzates of high solids loading ammonia fiber expansion-pretreated corn stover (18% weight per volume solids) and dilute sulfuric acid-pretreated switchgrass (20% w/v solids) were obtained. Improved features include reduced initial lag phase preceding growth, significantly enhanced fermentation rates, improved ethanol tolerance and yield, reduced diauxic lag during glucose-xylose transition, and ability to accumulate >40 g/L ethanol in <167 h when fermenting hydrolyzate at low initial cell density of 0.5 absorbance units and pH 5 to 6.

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Research Organization:: Great Lakes Bioenergy Research Center (GLBRC), Michigan State Univ., East Lansing, MI (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: FC02-07ER64494

OSTI ID:: 1184797

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Journal Issue: 1 Vol. 8; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Repression of xylose-specific enzymes by ethanol in Scheffersomyces (Pichia) stipitis and utility of repitching xylose-grown populations to eliminate diauxic lag Slininger, Patricia J.; Thompson, Stephanie R.; Weber, Scott Biotechnology and Bioengineering, Vol. 108, Issue 8 https://doi.org/10.1002/bit.23119	journal	March 2011
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Adaptive response of yeasts to furfural and 5-hydroxymethylfurfural and new chemical evidence for HMF conversion to 2,5-bis-hydroxymethylfuran Liu, Z. L.; Slininger, P. J.; Dien, B. S. Journal of Industrial Microbiology & Biotechnology, Vol. 31, Issue 8, p. 345-352 https://doi.org/10.1007/s10295-004-0148-3	journal	July 2004
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Optimization of cellulolytic enzyme components through engineering Trichoderma reesei and on-site fermentation using the soluble inducer for cellulosic ethanol production from corn stover Li, Yong-Hao; Zhang, Xiao-Yue; Zhang, Fei Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1048-5	journal	February 2018
Comparative lipid production by oleaginous yeasts in hydrolyzates of lignocellulosic biomass and process strategy for high titers: High Titer Lipid Production by Yeasts in Lignocellulosic Hydrolyzates Slininger, Patricia J.; Dien, Bruce S.; Kurtzman, Cletus P. Biotechnology and Bioengineering, Vol. 113, Issue 8 https://doi.org/10.1002/bit.25928	journal	February 2016
Adaptive laboratory evolution of nanocellulose‐producing bacterium Vasconcellos, Vanessa M.; Farinas, Cristiane S.; Ximenes, Eduardo Biotechnology and Bioengineering, Vol. 116, Issue 8 https://doi.org/10.1002/bit.26997	journal	May 2019
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Title: Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading

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