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Title: Active site and laminarin binding in glycoside hydrolase family 55

Abstract

The Carbohydrate Active Enzyme (CAZy) database indicates that glycoside hydrolase family 55 (GH55) contains both endo- and exo-β-1,3-glucanases. The founding structure in the GH55 is PcLam55A from the white rot fungus Phanerochaete chrysosporium. Here, we present high resolution crystal structures of bacterial SacteLam55A from the highly cellulolytic Streptomyces sp. SirexAA-E with bound substrates and product. These structures, along with mutagenesis and kinetic studies, implicate Glu-502 as the catalytic acid (as proposed earlier for Glu-663 in PcLam55A) and a proton relay network of four residues in activating water as the nucleophile. Further, a set of conserved aromatic residues that define the active site apparently enforce an exo-glucanase reactivity as demonstrated by exhaustive hydrolysis reactions with purified laminarioligosaccharides. Two additional aromatic residues that line the substrate-binding channel show substrate-dependent conformational flexibility that may promote processive reactivity of the bound oligosaccharide in the bacterial enzymes. Gene synthesis carried out on ~30% of the GH55 family gave 34 active enzymes (19% functional coverage of the nonredundant members of GH55). These active enzymes reacted with only laminarin from a panel of 10 different soluble and insoluble polysaccharides and displayed a broad range of specific activities and optima for pH and temperature. Furthermore, application of thismore » experimental method provides a new, systematic way to annotate glycoside hydrolase phylogenetic space for functional properties.« less

Authors:
 [1];  [2];  [3];  [4];  [5];  [4];  [4]
  1. Univ. of Wisconsin, Madison, WI (United States); Univ. of Wisconsin, Oshkosh, WI (United States)
  2. Univ. of Wisconsin, Madison, WI (United States); Hokkaido Univ., Sapporo (Japan)
  3. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  4. Univ. of Wisconsin, Madison, WI (United States)
  5. California State Univ., Fullerton, CA (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1182778
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Biological Chemistry
Additional Journal Information:
Journal Volume: 290; Journal Issue: 19; Journal ID: ISSN 0021-9258
Publisher:
American Society for Biochemistry and Molecular Biology
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; genomics; glycoside hydrolase; hydrolase; proteomics; x-ray crystallography; β-1,3-glucanase; GH55; cell-free translation; exo-glucanase

Citation Formats

Bianchetti, Christopher M., Takasuka, Taichi E., Deutsch, Sam, Udell, Hannah S., Yik, Eric J., Bergeman, Lai F., and Fox, Brian G. Active site and laminarin binding in glycoside hydrolase family 55. United States: N. p., 2015. Web. doi:10.1074/jbc.M114.623579.
Bianchetti, Christopher M., Takasuka, Taichi E., Deutsch, Sam, Udell, Hannah S., Yik, Eric J., Bergeman, Lai F., & Fox, Brian G. Active site and laminarin binding in glycoside hydrolase family 55. United States. doi:10.1074/jbc.M114.623579.
Bianchetti, Christopher M., Takasuka, Taichi E., Deutsch, Sam, Udell, Hannah S., Yik, Eric J., Bergeman, Lai F., and Fox, Brian G. Mon . "Active site and laminarin binding in glycoside hydrolase family 55". United States. doi:10.1074/jbc.M114.623579. https://www.osti.gov/servlets/purl/1182778.
@article{osti_1182778,
title = {Active site and laminarin binding in glycoside hydrolase family 55},
author = {Bianchetti, Christopher M. and Takasuka, Taichi E. and Deutsch, Sam and Udell, Hannah S. and Yik, Eric J. and Bergeman, Lai F. and Fox, Brian G.},
abstractNote = {The Carbohydrate Active Enzyme (CAZy) database indicates that glycoside hydrolase family 55 (GH55) contains both endo- and exo-β-1,3-glucanases. The founding structure in the GH55 is PcLam55A from the white rot fungus Phanerochaete chrysosporium. Here, we present high resolution crystal structures of bacterial SacteLam55A from the highly cellulolytic Streptomyces sp. SirexAA-E with bound substrates and product. These structures, along with mutagenesis and kinetic studies, implicate Glu-502 as the catalytic acid (as proposed earlier for Glu-663 in PcLam55A) and a proton relay network of four residues in activating water as the nucleophile. Further, a set of conserved aromatic residues that define the active site apparently enforce an exo-glucanase reactivity as demonstrated by exhaustive hydrolysis reactions with purified laminarioligosaccharides. Two additional aromatic residues that line the substrate-binding channel show substrate-dependent conformational flexibility that may promote processive reactivity of the bound oligosaccharide in the bacterial enzymes. Gene synthesis carried out on ~30% of the GH55 family gave 34 active enzymes (19% functional coverage of the nonredundant members of GH55). These active enzymes reacted with only laminarin from a panel of 10 different soluble and insoluble polysaccharides and displayed a broad range of specific activities and optima for pH and temperature. Furthermore, application of this experimental method provides a new, systematic way to annotate glycoside hydrolase phylogenetic space for functional properties.},
doi = {10.1074/jbc.M114.623579},
journal = {Journal of Biological Chemistry},
number = 19,
volume = 290,
place = {United States},
year = {2015},
month = {3}
}

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