Active site and laminarin binding in glycoside hydrolase family 55

Bianchetti, Christopher M.; Takasuka, Taichi E.; Deutsch, Sam; Udell, Hannah S.; Yik, Eric J.; Bergeman, Lai F.; Fox, Brian G.

doi:10.1074/jbc.M114.623579

Title: Active site and laminarin binding in glycoside hydrolase family 55

Journal Article · 09 March 2015 · Journal of Biological Chemistry

DOI: https://doi.org/10.1074/jbc.M114.623579 · OSTI ID:1182778

Bianchetti, Christopher M. ^[1]; Takasuka, Taichi E. ^[2]; Deutsch, Sam ^[3]; Udell, Hannah S. ^[4]; Yik, Eric J. ^[5]; Bergeman, Lai F. ^[4]; Fox, Brian G. ^[4]

Univ. of Wisconsin, Madison, WI (United States); Univ. of Wisconsin, Oshkosh, WI (United States)
Univ. of Wisconsin, Madison, WI (United States); Hokkaido Univ., Sapporo (Japan)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Univ. of Wisconsin, Madison, WI (United States)
California State Univ., Fullerton, CA (United States)

The Carbohydrate Active Enzyme (CAZy) database indicates that glycoside hydrolase family 55 (GH55) contains both endo- and exo-β-1,3-glucanases. The founding structure in the GH55 is PcLam55A from the white rot fungus Phanerochaete chrysosporium. Here, we present high resolution crystal structures of bacterial SacteLam55A from the highly cellulolytic Streptomyces sp. SirexAA-E with bound substrates and product. These structures, along with mutagenesis and kinetic studies, implicate Glu-502 as the catalytic acid (as proposed earlier for Glu-663 in PcLam55A) and a proton relay network of four residues in activating water as the nucleophile. Further, a set of conserved aromatic residues that define the active site apparently enforce an exo-glucanase reactivity as demonstrated by exhaustive hydrolysis reactions with purified laminarioligosaccharides. Two additional aromatic residues that line the substrate-binding channel show substrate-dependent conformational flexibility that may promote processive reactivity of the bound oligosaccharide in the bacterial enzymes. Gene synthesis carried out on ~30% of the GH55 family gave 34 active enzymes (19% functional coverage of the nonredundant members of GH55). These active enzymes reacted with only laminarin from a panel of 10 different soluble and insoluble polysaccharides and displayed a broad range of specific activities and optima for pH and temperature. Furthermore, application of this experimental method provides a new, systematic way to annotate glycoside hydrolase phylogenetic space for functional properties.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI ID:: 1182778

Journal Information:: Journal of Biological Chemistry, Vol. 290, Issue 19; ISSN 0021-9258

Publisher:: American Society for Biochemistry and Molecular BiologyCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

Citation Metrics:

Cited by: 29 works

Citation information provided by
Web of Science

References (75)

Evolution of substrate specificity in bacterial AA10 lytic polysaccharide monooxygenases Book, Adam J.; Yennamalli, Ragothaman M.; Takasuka, Taichi E. Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-109	journal	January 2014
Cloning and characterization of multiple glycosyl hydrolase genes from Trichoderma virens Kim, Dong-Jin; Baek, Jong-Min; Uribe, Pedro Current Genetics, Vol. 40, Issue 6 https://doi.org/10.1007/s00294-001-0267-6	journal	March 2002
Glycosidase mechanisms Rye, Carl S.; Withers, Stephen G. Current Opinion in Chemical Biology, Vol. 4, Issue 5 https://doi.org/10.1016/S1367-5931(00)00135-6	journal	October 2000
Purification and Characterization of Laminaran Hydrolases from Trichoderma viride Nobe, Rika; Sakakibara, Yoichi; Fukuda, Nobuhiro Bioscience, Biotechnology, and Biochemistry, Vol. 67, Issue 6 https://doi.org/10.1271/bbb.67.1349	journal	January 2003
Molecular characterization of a novel β-1,3-exoglucanase related to mycoparasitism of Trichoderma harzianum Cohen-Kupiec, Rachel; Broglie, Karen E.; Friesem, Dana Gene, Vol. 226, Issue 2 https://doi.org/10.1016/S0378-1119(98)00583-6	journal	January 1999
Fusion of Dioxygenase and Lignin-binding Domains in a Novel Secreted Enzyme from Cellulolytic Streptomyces sp. SirexAA-E Bianchetti, Christopher M.; Harmann, Connor H.; Takasuka, Taichi E. Journal of Biological Chemistry, Vol. 288, Issue 25 https://doi.org/10.1074/jbc.M113.475848	journal	May 2013
Flexi Vector Cloning Blommel, Paul G.; Martin, Peter A.; Seder, Kory D. Methods in Molecular Biology https://doi.org/10.1007/978-1-59745-196-3_4	book	January 2009
Binding Site Dynamics and Aromatic–Carbohydrate Interactions in Processive and Non-Processive Family 7 Glycoside Hydrolases Taylor, Courtney B.; Payne, Christina M.; Himmel, Michael E. The Journal of Physical Chemistry B, Vol. 117, Issue 17 https://doi.org/10.1021/jp401410h	journal	April 2013
Ensemble Refinement of Protein Crystal Structures: Validation and Application Levin, Elena J.; Kondrashov, Dmitry A.; Wesenberg, Gary E. Structure, Vol. 15, Issue 9 https://doi.org/10.1016/j.str.2007.06.019	journal	September 2007
The Active Site of Cellobiohydrolase Cel6A from Trichoderma r eesei : The Roles of Aspartic Acids D221 and D175 Koivula, Anu; Ruohonen, Laura; Wohlfahrt, Gerd Journal of the American Chemical Society, Vol. 124, Issue 34 https://doi.org/10.1021/ja012659q	journal	August 2002
Structures of an active-site mutant of a plant 1,3-β-glucanase in complex with oligosaccharide products of hydrolysis Wojtkowiak, Agnieszka; Witek, Kamil; Hennig, Jacek Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 1 https://doi.org/10.1107/S0907444912042175	journal	December 2012
Iterative model building, structure refinement and density modification with the PHENIX AutoBuild wizard Terwilliger, Thomas C.; Grosse-Kunstleve, Ralf W.; Afonine, Pavel V. Acta Crystallographica Section D Biological Crystallography, Vol. 64, Issue 1 https://doi.org/10.1107/S090744490705024X	journal	December 2007
Substructure search procedures for macromolecular structures Grosse-Kunstleve, R. W.; Adams, P. D. Acta Crystallographica Section D Biological Crystallography, Vol. 59, Issue 11 https://doi.org/10.1107/S0907444903018043	journal	October 2003
Wounding in the plant tissue: the defense of a dangerous passage Savatin, Daniel V.; Gramegna, Giovanna; Modesti, Vanessa Frontiers in Plant Science, Vol. 5 https://doi.org/10.3389/fpls.2014.00470	journal	September 2014
Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts Klock, Heath E.; Koesema, Eric J.; Knuth, Mark W. Proteins: Structure, Function, and Bioinformatics, Vol. 71, Issue 2 https://doi.org/10.1002/prot.21786	journal	January 2008
Autoinduction of Protein Expression Fox, Brian G.; Blommel, Paul G. Current Protocols in Protein Science, Vol. 56, Issue 1 https://doi.org/10.1002/0471140864.ps0523s56	journal	April 2009
Aromatic Residues in the Catalytic Center of Chitinase A from Serratia marcescens Affect Processivity, Enzyme Activity, and Biomass Converting Efficiency Zakariassen, Henrik; Aam, Berit Bjugan; Horn, Svein J. Journal of Biological Chemistry, Vol. 284, Issue 16 https://doi.org/10.1074/jbc.M900092200	journal	February 2009
Acquisition of Digestive Enzymes by Siricid Woodwasps from Their Fungal Symbiont Kukor, J. J.; Martin, M. M. Science, Vol. 220, Issue 4602 https://doi.org/10.1126/science.220.4602.1161	journal	June 1983
Site-Directed Mutation of the Putative Catalytic Residues of Endoglucanase CenA from Cellulomonas fimi Damude, Howard Glenn; Withers, Stephen G.; Kilburn, Douglas G. Biochemistry, Vol. 34, Issue 7 https://doi.org/10.1021/bi00007a016	journal	February 1995
Cellulolytic Streptomyces Strains Associated with Herbivorous Insects Share a Phylogenetically Linked Capacity To Degrade Lignocellulose Book, Adam J.; Lewin, Gina R.; McDonald, Bradon R. Applied and Environmental Microbiology, Vol. 80, Issue 15 https://doi.org/10.1128/AEM.01133-14	journal	May 2014
SignalP 4.0: discriminating signal peptides from transmembrane regions Petersen, Thomas Nordahl; Brunak, Søren; von Heijne, Gunnar Nature Methods, Vol. 8, Issue 10 https://doi.org/10.1038/nmeth.1701	journal	September 2011
Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces Takasuka, Taichi E.; Book, Adam J.; Lewin, Gina R. Scientific Reports, Vol. 3, Issue 1 https://doi.org/10.1038/srep01030	journal	January 2013
Callose synthesis in higher plants Chen, Xiong-Yan; Kim, Jae-Yean Plant Signaling & Behavior, Vol. 4, Issue 6 https://doi.org/10.4161/psb.4.6.8359	journal	June 2009
Cellulose-degrading bacteria associated with the invasive woodwasp Sirex noctilio Adams, Aaron S.; Jordan, Michelle S.; Adams, Sandye M. The ISME Journal, Vol. 5, Issue 8 https://doi.org/10.1038/ismej.2011.14	journal	March 2011
Decision-making in structure solution using Bayesian estimates of map quality: the PHENIX AutoSol wizard Terwilliger, Thomas C.; Adams, Paul D.; Read, Randy J. Acta Crystallographica Section D Biological Crystallography, Vol. 65, Issue 6 https://doi.org/10.1107/S0907444909012098	journal	May 2009
Analysis of surface binding sites (SBSs) in carbohydrate active enzymes with focus on glycoside hydrolase families 13 and 77 — a mini-review Cockburn, Darrell; Wilkens, Casper; Ruzanski, Christian Biologia, Vol. 69, Issue 6 https://doi.org/10.2478/s11756-014-0373-9	journal	January 2014
The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics Cantarel, B. L.; Coutinho, P. M.; Rancurel, C. Nucleic Acids Research, Vol. 37, Issue Database https://doi.org/10.1093/nar/gkn663	journal	January 2009
Convergent Bacterial Microbiotas in the Fungal Agricultural Systems of Insects Aylward, Frank O.; Suen, Garret; Biedermann, Peter H. W. mBio, Vol. 5, Issue 6 https://doi.org/10.1128/mBio.02077-14	journal	October 2014
Cloning and Characterization of the Gene Encoding Endo-β-1,3-glucanase from Arthrobacter sp. NHB-10 Okazaki, Katsuichiro; Nishimura, Naohito; Matsuoka, Fumiyoshi Bioscience, Biotechnology, and Biochemistry, Vol. 71, Issue 6 https://doi.org/10.1271/bbb.70030	journal	June 2007
Modelling dynamics in protein crystal structures by ensemble refinement Burnley, B. Tom; Afonine, Pavel V.; Adams, Paul D. eLife, Vol. 1 https://doi.org/10.7554/eLife.00311	journal	December 2012
Inference of Macromolecular Assemblies from Crystalline State Krissinel, Evgeny; Henrick, Kim Journal of Molecular Biology, Vol. 372, Issue 3 https://doi.org/10.1016/j.jmb.2007.05.022	journal	September 2007
Crystal Structure of Glycoside Hydrolase Family 55 β-1,3-Glucanase from the Basidiomycete Phanerochaete chrysosporium Ishida, Takuya; Fushinobu, Shinya; Kawai, Rie Journal of Biological Chemistry, Vol. 284, Issue 15 https://doi.org/10.1074/jbc.M808122200	journal	February 2009
Multiple Functions of Aromatic-Carbohydrate Interactions in a Processive Cellulase Examined with Molecular Simulation Payne, Christina M.; Bomble, Yannick J.; Taylor, Courtney B. Journal of Biological Chemistry, Vol. 286, Issue 47 https://doi.org/10.1074/jbc.M111.297713	journal	September 2011
Predicting the potential distribution of Sirex noctilio (Hymenoptera: Siricidae), a significant exotic pest of Pinus plantations Carnegie, Angus J.; Matsuki, Mamoru; Haugen, Dennis A. Annals of Forest Science, Vol. 63, Issue 2 https://doi.org/10.1051/forest:2005104	journal	March 2006
ExPASy: the proteomics server for in-depth protein knowledge and analysis Gasteiger, E. Nucleic Acids Research, Vol. 31, Issue 13 https://doi.org/10.1093/nar/gkg563	journal	July 2003
The Tryptophan Residue at the Active Site Tunnel Entrance of Trichoderma reesei Cellobiohydrolase Cel7A Is Important for Initiation of Degradation of Crystalline Cellulose Nakamura, Akihiko; Tsukada, Takeshi; Auer, Sanna Journal of Biological Chemistry, Vol. 288, Issue 19 https://doi.org/10.1074/jbc.M113.452623	journal	March 2013
Structure, Mechanistic Action, and Essential Residues of a GH-64 Enzyme, Laminaripentaose-producing β-1,3-Glucanase Wu, Hsin-Mao; Liu, Sheng-Wen; Hsu, Ming-Tsung Journal of Biological Chemistry, Vol. 284, Issue 39 https://doi.org/10.1074/jbc.M109.010983	journal	July 2009
A novel endo-beta-1,3-glucanase, BGN13.1, involved in the mycoparasitism of Trichoderma harzianum. de la Cruz, J.; Pintor-Toro, J. A.; Benítez, T. Journal of bacteriology, Vol. 177, Issue 23 https://doi.org/10.1128/jb.177.23.6937-6945.1995	journal	January 1995
Cloning, sequence and structure of a gene encoding an antifungal glucan 1,3-β-glucosidase from Trichoderma atroviride (T. harzianum) Donzelli, B. Giuliano Garisto; Lorito, M.; Scala, F. Gene, Vol. 277, Issue 1-2 https://doi.org/10.1016/S0378-1119(01)00681-3	journal	October 2001
A Secondary Xylan-binding Site Enhances the Catalytic Activity of a Single-domain Family 11 Glycoside Hydrolase Ludwiczek, Martin L.; Heller, Markus; Kantner, Terrence Journal of Molecular Biology, Vol. 373, Issue 2 https://doi.org/10.1016/j.jmb.2007.07.057	journal	October 2007
142. The structure of laminarin. Part II. The minor structural features Peat, Stanley; Whelan, W. J.; Lawley, H. G. Journal of the Chemical Society (Resumed) https://doi.org/10.1039/jr9580000729	journal	January 1958
The β-Glucanase Zg LamA from Zobellia galactanivorans Evolved a Bent Active Site Adapted for Efficient Degradation of Algal Laminarin Labourel, Aurore; Jam, Murielle; Jeudy, Alexandra Journal of Biological Chemistry, Vol. 289, Issue 4 https://doi.org/10.1074/jbc.M113.538843	journal	December 2013
Glycoside hydrolases: Catalytic base/nucleophile diversity Vuong, Thu V.; Wilson, David B. Biotechnology and Bioengineering, Vol. 107, Issue 2, p. 195-205 https://doi.org/10.1002/bit.22838	journal	June 2010
Structural and Functional Analysis of a Glycoside Hydrolase Family 97 Enzyme from Bacteroides thetaiotaomicron Kitamura, Momoyo; Okuyama, Masayuki; Tanzawa, Fumiko Journal of Biological Chemistry, Vol. 283, Issue 52 https://doi.org/10.1074/jbc.M806115200	journal	November 2008
Arthrobacter phenanthrenivorans sp. nov., to accommodate the phenanthrene-degrading bacterium Arthrobacter sp. strain Sphe3 Kallimanis, A.; Kavakiotis, K.; Perisynakis, A. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 59, Issue 2 https://doi.org/10.1099/ijs.0.000984-0	journal	February 2009
MolProbity : all-atom structure validation for macromolecular crystallography Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1 https://doi.org/10.1107/S0907444909042073	journal	December 2009
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar Miller, G. L. Analytical Chemistry, Vol. 31, Issue 3 https://doi.org/10.1021/ac60147a030	journal	March 1959
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
GeneDesign 3.0 is an updated synthetic biology toolkit Richardson, Sarah M.; Nunley, Paul W.; Yarrington, Robert M. Nucleic Acids Research, Vol. 38, Issue 8 https://doi.org/10.1093/nar/gkq143	journal	March 2010
Carbohydrate-binding modules: fine-tuning polysaccharide recognition Boraston, Alisdair B.; Bolam, David N.; Gilbert, Harry J. Biochemical Journal, Vol. 382, Issue 3 https://doi.org/10.1042/BJ20040892	journal	September 2004
Biochemical Properties and Atomic Resolution Structure of a Proteolytically Processed β-Mannanase from Cellulolytic Streptomyces sp. SirexAA-E Takasuka, Taichi E.; Acheson, Justin F.; Bianchetti, Christopher M. PLoS ONE, Vol. 9, Issue 4 https://doi.org/10.1371/journal.pone.0094166	journal	April 2014
The architecture of parallel β-helices and related folds Jenkins, John; Pickersgill, Richard Progress in Biophysics and Molecular Biology, Vol. 77, Issue 2 https://doi.org/10.1016/S0079-6107(01)00013-X	journal	October 2001
Analysis of the structural heterogeneity of laminarin by electrospray-ionisation-mass spectrometry Read, Steve M.; Currie, Graeme; Bacic, Antony Carbohydrate Research, Vol. 281, Issue 2 https://doi.org/10.1016/0008-6215(95)00350-9	journal	February 1996
Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite Warnecke, Falk; Luginbühl, Peter; Ivanova, Natalia Nature, Vol. 450, Issue 7169 https://doi.org/10.1038/nature06269	journal	November 2007
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
A combined approach to improving large-scale production of tobacco etch virus protease Blommel, Paul G.; Fox, Brian G. Protein Expression and Purification, Vol. 55, Issue 1, p. 53-68 https://doi.org/10.1016/j.pep.2007.04.013	journal	September 2007
Enzymatic Assembly of Overlapping DNA Fragments Gibson, Daniel G. Methods in Enzymology https://doi.org/10.1016/B978-0-12-385120-8.00015-2	book	May 2011
Enhanced Bacterial Protein Expression During Auto-Induction Obtained by Alteration of Lac Repressor Dosage and Medium Composition Blommel, Paul G.; Becker, Katie J.; Duvnjak, Petar Biotechnology Progress, Vol. 23, Issue 3 https://doi.org/10.1021/bp070011x	journal	January 2007
Mechanisms of enzymatic glycoside hydrolysis McCarter, John D.; Stephen Withers, G. Current Opinion in Structural Biology, Vol. 4, Issue 6 https://doi.org/10.1016/0959-440X(94)90271-2	journal	January 1994
Cloning and Expression of a Novel Trichoderma viride Laminarinase AI Gene ( lamAI ) Nobe, Rika; Sakakibara, Yoichi; Ogawa, Kihachiro Bioscience, Biotechnology, and Biochemistry, Vol. 68, Issue 10 https://doi.org/10.1271/bbb.68.2111	journal	January 2004
Cell-Free Translation of Biofuel Enzymes Takasuka, Taichi E.; Walker, Johnnie A.; Bergeman, Lai F. Methods in Molecular Biology https://doi.org/10.1007/978-1-62703-782-2_5	book	December 2013
NMRPipe: A multidimensional spectral processing system based on UNIX pipes Delaglio, Frank; Grzesiek, Stephan; Vuister, GeertenW. Journal of Biomolecular NMR, Vol. 6, Issue 3 https://doi.org/10.1007/BF00197809	journal	November 1995
Expression of cmg1, an Exo-β-1,3-Glucanase Gene from Coniothyrium minitans, Increases during Sclerotial Parasitism Giczey, Gábor; Kerényi, Zoltán; Fülöp, László Applied and Environmental Microbiology, Vol. 67, Issue 2 https://doi.org/10.1128/AEM.67.2.865-871.2001	journal	February 2001
Gene Cloning and Heterologous Expression of Glycoside Hydrolase Family 55 β-1,3-Glucanase from the Basidiomycete Phanerochaete Chrysosporium Kawai, Rie; Igarashi, Kiyohiko; Samejima, Masahiro Biotechnology Letters, Vol. 28, Issue 6 https://doi.org/10.1007/s10529-005-6179-7	journal	March 2006
Cloning and targeted gene disruption of EXG1, encoding exo-beta 1, 3-glucanase, in the phytopathogenic fungus Cochliobolus carbonum. Schaeffer, H. J.; Leykam, J.; Walton, J. D. Applied and Environmental Microbiology, Vol. 60, Issue 2 https://doi.org/10.1128/AEM.60.2.594-598.1994	journal	January 1994
Nomenclature for sugar-binding subsites in glycosyl hydrolases Davies, Gideon J.; Wilson, Keith S.; Henrissat, Bernard Biochemical Journal, Vol. 321, Issue 2 https://doi.org/10.1042/bj3210557	journal	January 1997
The absence of an identifiable single catalytic base residue in Thermobifida fusca exocellulase Cel6B Vuong, Thu V.; Wilson, David B. FEBS Journal, Vol. 276, Issue 14 https://doi.org/10.1111/j.1742-4658.2009.07097.x	journal	July 2009
The cell wall: a carbohydrate armour for the fungal cell Latgé, Jean-Paul Molecular Microbiology, Vol. 66, Issue 2 https://doi.org/10.1111/j.1365-2958.2007.05872.x	journal	October 2007
The fungus gardens of leaf-cutter ants undergo a distinct physiological transition during biomass degradation: Metabolomics of leaf-cutter ant fungus gardens Huang, Eric L.; Aylward, Frank O.; Kim, Young-Mo Environmental Microbiology Reports, Vol. 6, Issue 4 https://doi.org/10.1111/1758-2229.12163	journal	April 2014
Clustal Omega, Accurate Alignment of Very Large Numbers of Sequences Sievers, Fabian; Higgins, Desmond G. Multiple Sequence Alignment Methods https://doi.org/10.1007/978-1-62703-646-7_6	book	January 2013
Antitumor anthraquinones from an endophytic actinomycete Micromonospora lupini sp. nov. Igarashi, Yasuhiro; Trujillo, Martha E.; Martínez-Molina, Eustoquio Bioorganic & Medicinal Chemistry Letters, Vol. 17, Issue 13 https://doi.org/10.1016/j.bmcl.2007.04.039	journal	July 2007
Phaser crystallographic software McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D. Journal of Applied Crystallography, Vol. 40, Issue 4 https://doi.org/10.1107/S0021889807021206	journal	July 2007
Metagenomic and metaproteomic insights into bacterial communities in leaf-cutter ant fungus gardens Aylward, Frank O.; Burnum, Kristin E.; Scott, Jarrod J. The ISME Journal, Vol. 6, Issue 9 https://doi.org/10.1038/ismej.2012.10	journal	March 2012
Bacterial symbionts in agricultural systems provide a strategic source for antibiotic discovery Ramadhar, Timothy R.; Beemelmanns, Christine; Currie, Cameron R. The Journal of Antibiotics, Vol. 67, Issue 1 https://doi.org/10.1038/ja.2013.77	journal	August 2013
[20] Processing of X-ray diffraction data collected in oscillation mode Otwinowski, Zbyszek; Minor, Wladek Macromolecular Crystallography Part A, 307-326 https://doi.org/10.1016/S0076-6879(97)76066-X	book	January 1997

Cited By (4)

Structural insights into substrate recognition and catalysis by glycoside hydrolase family 87 α‐1,3‐glucanase from Paenibacillus glycanilyticus FH11 Itoh, Takafumi; Intuy, Rattanaporn; Suyotha, Wasana The FEBS Journal, Vol. 287, Issue 12 https://doi.org/10.1111/febs.15161	journal	December 2019
Function-driven single-cell genomics uncovers cellulose-degrading bacteria from the rare biosphere Doud, Devin F. R.; Bowers, Robert M.; Schulz, Frederik The ISME Journal, Vol. 14, Issue 3 https://doi.org/10.1038/s41396-019-0557-y	journal	November 2019
Identification, characterization, and structural analyses of a fungal endo-β-1,2-glucanase reveal a new glycoside hydrolase family Tanaka, Nobukiyo; Nakajima, Masahiro; Narukawa-Nara, Megumi Journal of Biological Chemistry, Vol. 294, Issue 19 https://doi.org/10.1074/jbc.ra118.007087	journal	March 2019
Eng1 and Exg8 Are the Major β-Glucanases Secreted by the Fungal Pathogen Histoplasma capsulatum Garfoot, Andrew L.; Dearing, Kacey L.; VanSchoiack, Andrew D. Journal of Biological Chemistry, Vol. 292, Issue 12 https://doi.org/10.1074/jbc.m116.762104	journal	February 2017

Similar Records

Insights into the dual cleavage activity of the GH16 laminarinase enzyme class on β-1,3 and β-1,4 glycosidic bonds

Journal Article · 2021 · Journal of Biological Chemistry · OSTI ID:1767876

Liberato, Marcelo Vizona; Prates, Erica Teixeira; Gonçalves, Thiago Augusto; +11 more

Accurate Quantification of Laminarin in Marine Organic Matter with Enzymes from Marine Microbes

Journal Article · 2017 · Applied and Environmental Microbiology · OSTI ID:1536860

Becker, Stefan; Scheffel, André; Polz, Martin F.; +1 more

Direct determination of protonation states and visualization of hydrogen bonding in a glycoside hydrolase with neutron crystallography

Journal Article · 2015 · Proceedings of the National Academy of Sciences of the United States of America · OSTI ID:1265915

Wan, Qun; Parks, Jerry M.; Hanson, B. Leif; +7 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
genomics
glycoside hydrolase
hydrolase
proteomics
x-ray crystallography
β-1
3-glucanase
GH55
cell-free translation
exo-glucanase

Title: Active site and laminarin binding in glycoside hydrolase family 55

Citation Formats

References (75)

Cited By (4)

Similar Records

Related Subjects