Functional reconstitution of Drosophila melanogaster NMJ glutamate receptors
Abstract
The Drosophila larval neuromuscular junction (NMJ), at which glutamate acts as the excitatory neurotransmitter, is a widely used model for genetic analysis of synapse function and development. Despite decades of study, the inability to reconstitute NMJ glutamate receptor function using heterologous expression systems has complicated the analysis of receptor function, such that it is difficult to resolve the molecular basis for compound phenotypes observed in mutant flies. In this paper, we find that Drosophila Neto functions as an essential component required for the function of NMJ glutamate receptors, permitting analysis of glutamate receptor responses in Xenopus oocytes. Finally, in combination with a crystallographic analysis of the GluRIIB ligand binding domain, we use this system to characterize the subunit dependence of assembly, channel block, and ligand selectivity for Drosophila NMJ glutamate receptors.
- Authors:
-
- National Inst. of Health, Bethesda, MD (United States). The Eunice Kennedy Shriver National Inst. of Child Health and Human Development. Program in Cellular Regulation and Metabolism
- National Inst. of Health, Bethesda, MD (United States). The Eunice Kennedy Shriver National Inst. of Child Health and Human Development. Lab. of Cellular and Molecular Neurophysiology
- Publication Date:
- Research Org.:
- National Inst. of Health, Bethesda, MD (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Inst. of Health (NIH) (United States)
- OSTI Identifier:
- 1182323
- Grant/Contract Number:
- W-31-109-Eng-38
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Volume: 112; Journal Issue: 19; Journal ID: ISSN 0027-8424
- Publisher:
- National Academy of Sciences, Washington, DC (United States)
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Drosophila; NMJ; Neto; glutamate receptors; crystallography
Citation Formats
Han, Tae Hee, Dharkar, Poorva, Mayer, Mark L., and Serpe, Mihaela. Functional reconstitution of Drosophila melanogaster NMJ glutamate receptors. United States: N. p., 2015.
Web. doi:10.1073/pnas.1500458112.
Han, Tae Hee, Dharkar, Poorva, Mayer, Mark L., & Serpe, Mihaela. Functional reconstitution of Drosophila melanogaster NMJ glutamate receptors. United States. https://doi.org/10.1073/pnas.1500458112
Han, Tae Hee, Dharkar, Poorva, Mayer, Mark L., and Serpe, Mihaela. Mon .
"Functional reconstitution of Drosophila melanogaster NMJ glutamate receptors". United States. https://doi.org/10.1073/pnas.1500458112. https://www.osti.gov/servlets/purl/1182323.
@article{osti_1182323,
title = {Functional reconstitution of Drosophila melanogaster NMJ glutamate receptors},
author = {Han, Tae Hee and Dharkar, Poorva and Mayer, Mark L. and Serpe, Mihaela},
abstractNote = {The Drosophila larval neuromuscular junction (NMJ), at which glutamate acts as the excitatory neurotransmitter, is a widely used model for genetic analysis of synapse function and development. Despite decades of study, the inability to reconstitute NMJ glutamate receptor function using heterologous expression systems has complicated the analysis of receptor function, such that it is difficult to resolve the molecular basis for compound phenotypes observed in mutant flies. In this paper, we find that Drosophila Neto functions as an essential component required for the function of NMJ glutamate receptors, permitting analysis of glutamate receptor responses in Xenopus oocytes. Finally, in combination with a crystallographic analysis of the GluRIIB ligand binding domain, we use this system to characterize the subunit dependence of assembly, channel block, and ligand selectivity for Drosophila NMJ glutamate receptors.},
doi = {10.1073/pnas.1500458112},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 19,
volume = 112,
place = {United States},
year = {Mon Apr 27 00:00:00 EDT 2015},
month = {Mon Apr 27 00:00:00 EDT 2015}
}
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