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Title: Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents

Abstract

Here, for the first time, we demonstrate formation of virus-like nanoparticles (VNPs) utilizing gold-coated iron oxide nanoparticles as cores and capsidprotein of brome mosaic virus (BMV) or hepatitis B virus (HBV) as shells. Further, utilizing cryo-electron microscopy and single particle methods, we are able to show that the BMV coat on VNPs assembles into a structure very close to that of a native virion. This is a consequence of an optimal iron oxide NP size (~11 nm) fitting the virus cavity and an ultrathin gold layer on the maghemite cores, which allows for utilization of SH-(CH 2) 11-(CH 2-CH 2-O) 4-OCH 2-COOH as capping molecules to provide sufficient stability, charge density, and small form factor. MRI studies show unique relaxivity ratios that diminish only slightly with gold coating. In conclusion, a virus protein coating of a magnetic core mimicking the wild-type virus makes these VNPs a versatile platform for biomedical applications.

Authors:
 [1];  [1];  [1];  [2];  [1];  [1];  [1];  [1];  [2];  [1];  [3]
+ Show Author Affiliations
  1. Indiana Univ., Bloomington, IN (United States)
  2. European Molecular Biology Lab. (EMBL), Hamburg (Germany)
  3. King Abdulaziz Univ., Jeddah (Saudi Arabia); Indiana Univ., Bloomington, IN (United States)
Publication Date:
Research Org.:
Indiana Univ., Bloomington, IN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1167524
Alternate Identifier(s):
OSTI ID: 1440972
Grant/Contract Number:  
SC0010507
Resource Type:
Published Article
Journal Name:
Chemistry of Materials
Additional Journal Information:
Journal Volume: 27; Journal Issue: 1; Journal ID: ISSN 0897-4756
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Malyutin, Andrey G., Easterday, Rosemary, Lozovyy, Yaroslav, Spilotros, Alessandro, Cheng, Hu, Sanchez-Felix, Olivia R., Stein, Barry D., Morgan, David Gene, Svergun, Dmitri I., Dragnea, Bogdan, and Bronstein, Lyudmila M. Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents. United States: N. p., 2014. Web. doi:10.1021/cm504029j.
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Malyutin, Andrey G., Easterday, Rosemary, Lozovyy, Yaroslav, Spilotros, Alessandro, Cheng, Hu, Sanchez-Felix, Olivia R., Stein, Barry D., Morgan, David Gene, Svergun, Dmitri I., Dragnea, Bogdan, & Bronstein, Lyudmila M. Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents. United States. doi:10.1021/cm504029j.
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Malyutin, Andrey G., Easterday, Rosemary, Lozovyy, Yaroslav, Spilotros, Alessandro, Cheng, Hu, Sanchez-Felix, Olivia R., Stein, Barry D., Morgan, David Gene, Svergun, Dmitri I., Dragnea, Bogdan, and Bronstein, Lyudmila M. Mon . "Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents". United States. doi:10.1021/cm504029j.
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@article{osti_1167524,
title = {Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents},
author = {Malyutin, Andrey G. and Easterday, Rosemary and Lozovyy, Yaroslav and Spilotros, Alessandro and Cheng, Hu and Sanchez-Felix, Olivia R. and Stein, Barry D. and Morgan, David Gene and Svergun, Dmitri I. and Dragnea, Bogdan and Bronstein, Lyudmila M.},
abstractNote = {Here, for the first time, we demonstrate formation of virus-like nanoparticles (VNPs) utilizing gold-coated iron oxide nanoparticles as cores and capsidprotein of brome mosaic virus (BMV) or hepatitis B virus (HBV) as shells. Further, utilizing cryo-electron microscopy and single particle methods, we are able to show that the BMV coat on VNPs assembles into a structure very close to that of a native virion. This is a consequence of an optimal iron oxide NP size (~11 nm) fitting the virus cavity and an ultrathin gold layer on the maghemite cores, which allows for utilization of SH-(CH2)11-(CH2-CH2-O)4-OCH2-COOH as capping molecules to provide sufficient stability, charge density, and small form factor. MRI studies show unique relaxivity ratios that diminish only slightly with gold coating. In conclusion, a virus protein coating of a magnetic core mimicking the wild-type virus makes these VNPs a versatile platform for biomedical applications.},
doi = {10.1021/cm504029j},
journal = {Chemistry of Materials},
number = 1,
volume = 27,
place = {United States},
year = {2014},
month = {12}
}
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Journal Article:
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Publisher's Version of Record
DOI: 10.1021/cm504029j
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