Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents
Abstract
Here, for the first time, we demonstrate formation of virus-like nanoparticles (VNPs) utilizing gold-coated iron oxide nanoparticles as cores and capsidprotein of brome mosaic virus (BMV) or hepatitis B virus (HBV) as shells. Further, utilizing cryo-electron microscopy and single particle methods, we are able to show that the BMV coat on VNPs assembles into a structure very close to that of a native virion. This is a consequence of an optimal iron oxide NP size (~11 nm) fitting the virus cavity and an ultrathin gold layer on the maghemite cores, which allows for utilization of SH-(CH2)11-(CH2-CH2-O)4-OCH2-COOH as capping molecules to provide sufficient stability, charge density, and small form factor. MRI studies show unique relaxivity ratios that diminish only slightly with gold coating. In conclusion, a virus protein coating of a magnetic core mimicking the wild-type virus makes these VNPs a versatile platform for biomedical applications.
- Authors:
-
- Department of Chemistry, Indiana University, 800 East Kirkwood Avenue, Bloomington, Indiana 47405, United States
- European Molecular Biology Laboratory (EMBL), Hamburg Outstation, Notkestraße 85, D-22603 Hamburg, Germany
- Department of Psychological and Brain Sciences, Indiana University, 1101 East Tenth Street, Bloomington, Indiana 47403, United States
- Department of Biology, Indiana University, 1001 East Third Street, Bloomington, Indiana 47405, United States
- Department of Chemistry, Indiana University, 800 East Kirkwood Avenue, Bloomington, Indiana 47405, United States, Department of Physics, Faculty of Science , King Abdulaziz University, Jeddah, Saudi Arabia
- Publication Date:
- Research Org.:
- Indiana Univ., Bloomington, IN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1167524
- Alternate Identifier(s):
- OSTI ID: 1440972
- Grant/Contract Number:
- SC0010507
- Resource Type:
- Published Article
- Journal Name:
- Chemistry of Materials
- Additional Journal Information:
- Journal Name: Chemistry of Materials Journal Volume: 27 Journal Issue: 1; Journal ID: ISSN 0897-4756
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Malyutin, Andrey G., Easterday, Rosemary, Lozovyy, Yaroslav, Spilotros, Alessandro, Cheng, Hu, Sanchez-Felix, Olivia R., Stein, Barry D., Morgan, David Gene, Svergun, Dmitri I., Dragnea, Bogdan, and Bronstein, Lyudmila M. Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents. United States: N. p., 2014.
Web. doi:10.1021/cm504029j.
Malyutin, Andrey G., Easterday, Rosemary, Lozovyy, Yaroslav, Spilotros, Alessandro, Cheng, Hu, Sanchez-Felix, Olivia R., Stein, Barry D., Morgan, David Gene, Svergun, Dmitri I., Dragnea, Bogdan, & Bronstein, Lyudmila M. Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents. United States. https://doi.org/10.1021/cm504029j
Malyutin, Andrey G., Easterday, Rosemary, Lozovyy, Yaroslav, Spilotros, Alessandro, Cheng, Hu, Sanchez-Felix, Olivia R., Stein, Barry D., Morgan, David Gene, Svergun, Dmitri I., Dragnea, Bogdan, and Bronstein, Lyudmila M. Tue .
"Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents". United States. https://doi.org/10.1021/cm504029j.
@article{osti_1167524,
title = {Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents},
author = {Malyutin, Andrey G. and Easterday, Rosemary and Lozovyy, Yaroslav and Spilotros, Alessandro and Cheng, Hu and Sanchez-Felix, Olivia R. and Stein, Barry D. and Morgan, David Gene and Svergun, Dmitri I. and Dragnea, Bogdan and Bronstein, Lyudmila M.},
abstractNote = {Here, for the first time, we demonstrate formation of virus-like nanoparticles (VNPs) utilizing gold-coated iron oxide nanoparticles as cores and capsidprotein of brome mosaic virus (BMV) or hepatitis B virus (HBV) as shells. Further, utilizing cryo-electron microscopy and single particle methods, we are able to show that the BMV coat on VNPs assembles into a structure very close to that of a native virion. This is a consequence of an optimal iron oxide NP size (~11 nm) fitting the virus cavity and an ultrathin gold layer on the maghemite cores, which allows for utilization of SH-(CH2)11-(CH2-CH2-O)4-OCH2-COOH as capping molecules to provide sufficient stability, charge density, and small form factor. MRI studies show unique relaxivity ratios that diminish only slightly with gold coating. In conclusion, a virus protein coating of a magnetic core mimicking the wild-type virus makes these VNPs a versatile platform for biomedical applications.},
doi = {10.1021/cm504029j},
journal = {Chemistry of Materials},
number = 1,
volume = 27,
place = {United States},
year = {2014},
month = {12}
}
https://doi.org/10.1021/cm504029j
Web of Science
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