Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents

Malyutin, Andrey G.; Easterday, Rosemary; Lozovyy, Yaroslav; Spilotros, Alessandro; Cheng, Hu; Sanchez-Felix, Olivia R.; Stein, Barry D.; Morgan, David Gene; Svergun, Dmitri I.; Dragnea, Bogdan; Bronstein, Lyudmila M.

doi:10.1021/cm504029j

Title: Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents

Journal Article · 15 December 2014 · Chemistry of Materials

DOI: https://doi.org/10.1021/cm504029j · OSTI ID:1167524

Malyutin, Andrey G. ^[1]; Easterday, Rosemary ^[1]; Lozovyy, Yaroslav ^[1]; Spilotros, Alessandro ^[2]; Cheng, Hu ^[1]; Sanchez-Felix, Olivia R. ^[1]; Stein, Barry D. ^[1]; Morgan, David Gene ^[1]; Svergun, Dmitri I. ^[2]; Dragnea, Bogdan ^[1]; Bronstein, Lyudmila M. ^[3]

Indiana Univ., Bloomington, IN (United States)
European Molecular Biology Lab. (EMBL), Hamburg (Germany)
King Abdulaziz Univ., Jeddah (Saudi Arabia); Indiana Univ., Bloomington, IN (United States)

Here, for the first time, we demonstrate formation of virus-like nanoparticles (VNPs) utilizing gold-coated iron oxide nanoparticles as cores and capsidprotein of brome mosaic virus (BMV) or hepatitis B virus (HBV) as shells. Further, utilizing cryo-electron microscopy and single particle methods, we are able to show that the BMV coat on VNPs assembles into a structure very close to that of a native virion. This is a consequence of an optimal iron oxide NP size (~11 nm) fitting the virus cavity and an ultrathin gold layer on the maghemite cores, which allows for utilization of SH-(CH₂)₁₁-(CH₂-CH₂-O)₄-OCH₂-COOH as capping molecules to provide sufficient stability, charge density, and small form factor. MRI studies show unique relaxivity ratios that diminish only slightly with gold coating. In conclusion, a virus protein coating of a magnetic core mimicking the wild-type virus makes these VNPs a versatile platform for biomedical applications.

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Research Organization:: Indiana Univ., Bloomington, IN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0010507

OSTI ID:: 1167524

Alternate ID(s):: OSTI ID: 1440972

Journal Information:: Chemistry of Materials, Vol. 27, Issue 1; ISSN 0897-4756

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 29 works

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