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Title: Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents

Abstract

Here, for the first time, we demonstrate formation of virus-like nanoparticles (VNPs) utilizing gold-coated iron oxide nanoparticles as cores and capsidprotein of brome mosaic virus (BMV) or hepatitis B virus (HBV) as shells. Further, utilizing cryo-electron microscopy and single particle methods, we are able to show that the BMV coat on VNPs assembles into a structure very close to that of a native virion. This is a consequence of an optimal iron oxide NP size (~11 nm) fitting the virus cavity and an ultrathin gold layer on the maghemite cores, which allows for utilization of SH-(CH2)11-(CH2-CH2-O)4-OCH2-COOH as capping molecules to provide sufficient stability, charge density, and small form factor. MRI studies show unique relaxivity ratios that diminish only slightly with gold coating. In conclusion, a virus protein coating of a magnetic core mimicking the wild-type virus makes these VNPs a versatile platform for biomedical applications.

Authors:
 [1];  [1];  [1];  [2];  [3];  [1];  [4];  [1];  [2];  [1];  [5]
  1. Department of Chemistry, Indiana University, 800 East Kirkwood Avenue, Bloomington, Indiana 47405, United States
  2. European Molecular Biology Laboratory (EMBL), Hamburg Outstation, Notkestraße 85, D-22603 Hamburg, Germany
  3. Department of Psychological and Brain Sciences, Indiana University, 1101 East Tenth Street, Bloomington, Indiana 47403, United States
  4. Department of Biology, Indiana University, 1001 East Third Street, Bloomington, Indiana 47405, United States
  5. Department of Chemistry, Indiana University, 800 East Kirkwood Avenue, Bloomington, Indiana 47405, United States, Department of Physics, Faculty of Science , King Abdulaziz University, Jeddah, Saudi Arabia
Publication Date:
Research Org.:
Indiana Univ., Bloomington, IN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1167524
Alternate Identifier(s):
OSTI ID: 1440972
Grant/Contract Number:  
SC0010507
Resource Type:
Published Article
Journal Name:
Chemistry of Materials
Additional Journal Information:
Journal Name: Chemistry of Materials Journal Volume: 27 Journal Issue: 1; Journal ID: ISSN 0897-4756
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Malyutin, Andrey G., Easterday, Rosemary, Lozovyy, Yaroslav, Spilotros, Alessandro, Cheng, Hu, Sanchez-Felix, Olivia R., Stein, Barry D., Morgan, David Gene, Svergun, Dmitri I., Dragnea, Bogdan, and Bronstein, Lyudmila M. Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents. United States: N. p., 2014. Web. doi:10.1021/cm504029j.
Malyutin, Andrey G., Easterday, Rosemary, Lozovyy, Yaroslav, Spilotros, Alessandro, Cheng, Hu, Sanchez-Felix, Olivia R., Stein, Barry D., Morgan, David Gene, Svergun, Dmitri I., Dragnea, Bogdan, & Bronstein, Lyudmila M. Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents. United States. doi:10.1021/cm504029j.
Malyutin, Andrey G., Easterday, Rosemary, Lozovyy, Yaroslav, Spilotros, Alessandro, Cheng, Hu, Sanchez-Felix, Olivia R., Stein, Barry D., Morgan, David Gene, Svergun, Dmitri I., Dragnea, Bogdan, and Bronstein, Lyudmila M. Tue . "Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents". United States. doi:10.1021/cm504029j.
@article{osti_1167524,
title = {Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents},
author = {Malyutin, Andrey G. and Easterday, Rosemary and Lozovyy, Yaroslav and Spilotros, Alessandro and Cheng, Hu and Sanchez-Felix, Olivia R. and Stein, Barry D. and Morgan, David Gene and Svergun, Dmitri I. and Dragnea, Bogdan and Bronstein, Lyudmila M.},
abstractNote = {Here, for the first time, we demonstrate formation of virus-like nanoparticles (VNPs) utilizing gold-coated iron oxide nanoparticles as cores and capsidprotein of brome mosaic virus (BMV) or hepatitis B virus (HBV) as shells. Further, utilizing cryo-electron microscopy and single particle methods, we are able to show that the BMV coat on VNPs assembles into a structure very close to that of a native virion. This is a consequence of an optimal iron oxide NP size (~11 nm) fitting the virus cavity and an ultrathin gold layer on the maghemite cores, which allows for utilization of SH-(CH2)11-(CH2-CH2-O)4-OCH2-COOH as capping molecules to provide sufficient stability, charge density, and small form factor. MRI studies show unique relaxivity ratios that diminish only slightly with gold coating. In conclusion, a virus protein coating of a magnetic core mimicking the wild-type virus makes these VNPs a versatile platform for biomedical applications.},
doi = {10.1021/cm504029j},
journal = {Chemistry of Materials},
number = 1,
volume = 27,
place = {United States},
year = {2014},
month = {12}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1021/cm504029j

Citation Metrics:
Cited by: 15 works
Citation information provided by
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