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Title: An assessment of the resolution limitation due to radiation-damage in X-ray diffraction microscopy

Abstract

X-ray diffraction microscopy (XDM) is a new form of x-ray imaging that is being practiced at several third-generation synchrotron-radiation x-ray facilities. Nine years have elapsed since the technique was first introduced and it has made rapid progress in demonstrating high-resolution three-dimensional imaging and promises few-nm resolution with much larger samples than can be imaged in the transmission electron microscope. Both life- and materials-science applications of XDM are intended, and it is expected that the principal limitation to resolution will be radiation damage for life science and the coherent power of available x-ray sources for material science. In this paper we address the question of the role of radiation damage. We use a statistical analysis based on the so-called "dose fractionation theorem" of Hegerl and Hoppe to calculate the dose needed to make an image of a single life-science sample by XDM with a given resolution. We find that for simply-shaped objects the needed dose scales with the inverse fourth power of the resolution and present experimental evidence to support this finding. To determine the maximum tolerable dose we have assembled a number of data taken from the literature plus some measurements of our own which cover ranges of resolution thatmore » are not well covered otherwise. The conclusion of this study is that, based on the natural contrast between protein and water and "Rose-criterion" image quality, one should be able to image a frozen-hydrated biological sample using XDM at a resolution of about 10 nm.« less

Authors:
 [1];  [2];  [3];  [1];  [4];  [5];  [5];  [2];  [1];  [2];  [2];  [1];  [6];  [7]
  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  2. Stony Brook Univ., NY (United States) Dept. of Physics
  3. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics
  5. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Stony Brook Univ., NY (United States) Dept. of Physics
  6. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Arizona State Univ., Tempe, AZ (United States) Dept. of Physics and Astronomy
  7. Arizona State Univ., Tempe, AZ (United States) Dept. of Physics and Astronomy
Publication Date:
Research Org.:
DOE-RFSUNY-46128
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1165056
Grant/Contract Number:  
FG02-04ER46128
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Electron Spectroscopy and Related Phenomena
Additional Journal Information:
Journal Volume: 170; Journal Issue: 1-3; Journal ID: ISSN 0368-2048
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 77 NANOSCIENCE AND NANOTECHNOLOGY

Citation Formats

Howells, M. R., Beetz, T., Chapman, H. N., Cui, C., Holton, J. M., Jacobsen, C. J., Kirz, J., Lima, E., Marchesini, S., Miao, H., Sayre, D., Shapiro, D. A., Spence, J. C.H., and Starodub, D.. An assessment of the resolution limitation due to radiation-damage in X-ray diffraction microscopy. United States: N. p., 2008. Web. https://doi.org/10.1016/j.elspec.2008.10.008.
Howells, M. R., Beetz, T., Chapman, H. N., Cui, C., Holton, J. M., Jacobsen, C. J., Kirz, J., Lima, E., Marchesini, S., Miao, H., Sayre, D., Shapiro, D. A., Spence, J. C.H., & Starodub, D.. An assessment of the resolution limitation due to radiation-damage in X-ray diffraction microscopy. United States. https://doi.org/10.1016/j.elspec.2008.10.008
Howells, M. R., Beetz, T., Chapman, H. N., Cui, C., Holton, J. M., Jacobsen, C. J., Kirz, J., Lima, E., Marchesini, S., Miao, H., Sayre, D., Shapiro, D. A., Spence, J. C.H., and Starodub, D.. Mon . "An assessment of the resolution limitation due to radiation-damage in X-ray diffraction microscopy". United States. https://doi.org/10.1016/j.elspec.2008.10.008. https://www.osti.gov/servlets/purl/1165056.
@article{osti_1165056,
title = {An assessment of the resolution limitation due to radiation-damage in X-ray diffraction microscopy},
author = {Howells, M. R. and Beetz, T. and Chapman, H. N. and Cui, C. and Holton, J. M. and Jacobsen, C. J. and Kirz, J. and Lima, E. and Marchesini, S. and Miao, H. and Sayre, D. and Shapiro, D. A. and Spence, J. C.H. and Starodub, D.},
abstractNote = {X-ray diffraction microscopy (XDM) is a new form of x-ray imaging that is being practiced at several third-generation synchrotron-radiation x-ray facilities. Nine years have elapsed since the technique was first introduced and it has made rapid progress in demonstrating high-resolution three-dimensional imaging and promises few-nm resolution with much larger samples than can be imaged in the transmission electron microscope. Both life- and materials-science applications of XDM are intended, and it is expected that the principal limitation to resolution will be radiation damage for life science and the coherent power of available x-ray sources for material science. In this paper we address the question of the role of radiation damage. We use a statistical analysis based on the so-called "dose fractionation theorem" of Hegerl and Hoppe to calculate the dose needed to make an image of a single life-science sample by XDM with a given resolution. We find that for simply-shaped objects the needed dose scales with the inverse fourth power of the resolution and present experimental evidence to support this finding. To determine the maximum tolerable dose we have assembled a number of data taken from the literature plus some measurements of our own which cover ranges of resolution that are not well covered otherwise. The conclusion of this study is that, based on the natural contrast between protein and water and "Rose-criterion" image quality, one should be able to image a frozen-hydrated biological sample using XDM at a resolution of about 10 nm.},
doi = {10.1016/j.elspec.2008.10.008},
journal = {Journal of Electron Spectroscopy and Related Phenomena},
number = 1-3,
volume = 170,
place = {United States},
year = {2008},
month = {11}
}

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Works referenced in this record:

Three-Dimensional Coherent X-Ray Diffraction Imaging of a Ceramic Nanofoam: Determination of Structural Deformation Mechanisms
journal, July 2008


Advances in synchrotron hard X-ray based imaging
journal, June 2008


Apparatus for X-ray diffraction microscopy and tomography of cryo specimens
journal, June 2005

  • Beetz, T.; Howells, M. R.; Jacobsen, C.
  • Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 545, Issue 1-2
  • DOI: 10.1016/j.nima.2004.12.040

Femtosecond diffractive imaging with a soft-X-ray free-electron laser
journal, November 2006

  • Chapman, Henry N.; Barty, Anton; Bogan, Michael J.
  • Nature Physics, Vol. 2, Issue 12
  • DOI: 10.1038/nphys461

High-resolution ab initio three-dimensional x-ray diffraction microscopy
journal, January 2006

  • Chapman, Henry N.; Barty, Anton; Marchesini, Stefano
  • Journal of the Optical Society of America A, Vol. 23, Issue 5
  • DOI: 10.1364/JOSAA.23.001179

Phase retrieval by iterated projections
journal, January 2003


Reconstruction of an object from the modulus of its Fourier transform
journal, January 1978


Radiation damage to crystalline biological molecules: current view
journal, November 2002


Progress in research into radiation damage in cryo-cooled macromolecular crystals
journal, December 2006


Characterization of Conditions Required for X-Ray Diffraction Experiments with Protein Microcrystals
journal, June 2000


Limitations to significant information in biological electron microscopy as a result of radiation damage
journal, August 1971


Radiation damage relative to transmission electron microscopy of biological specimens at low temperature: a review
journal, January 1978


Radiation damage of purple membrane at low temperature
journal, January 1979


Influence of Electron Noise on Three-dimensional Image Reconstruction
journal, December 1976


Realizing the potential of electron cryo-microscopy
journal, February 2004


Submicroscopic Structure Determination by Long Wavelength X‐Ray Diffraction
journal, July 1955

  • Henke, Burton L.; DuMond, Jesse W. M.
  • Journal of Applied Physics, Vol. 26, Issue 7
  • DOI: 10.1063/1.1722117

Soft X-ray microscopes and their biological applications
journal, February 1995

  • Kirz, Janos; Jacobsen, Chris; Howells, Malcolm
  • Quarterly Reviews of Biophysics, Vol. 28, Issue 1
  • DOI: 10.1017/S0033583500003139

Wavelength choice for soft x-ray laser holography of biological samples
journal, January 1989

  • London, Richard A.; Rosen, Mordecai D.; Trebes, James E.
  • Applied Optics, Vol. 28, Issue 16
  • DOI: 10.1364/AO.28.003397

Coherent X-ray diffractive imaging: applications and limitations
journal, January 2003

  • Marchesini, S.; Chapman, H. N.; Hau-Riege, S. P.
  • Optics Express, Vol. 11, Issue 19
  • DOI: 10.1364/OE.11.002344

X-ray image reconstruction from a diffraction pattern alone
journal, October 2003


The relevance of dose-fractionation in tomography of radiation-sensitive specimens
journal, October 1995


Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Tomography
journal, November 2002


Taking X-Ray Diffraction to the Limit: Macromolecular Structures from Femtosecond X-Ray Pulses and Diffraction Microscopy of Cells with Synchrotron Radiation
journal, June 2004


Extending the methodology of X-ray crystallography to allow imaging of micrometre-sized non-crystalline specimens
journal, July 1999

  • Miao, Jianwei; Charalambous, Pambos; Kirz, Janos
  • Nature, Vol. 400, Issue 6742
  • DOI: 10.1038/22498

Three-Dimensional GaN Ga 2 O 3 Core Shell Structure Revealed by X-Ray Diffraction Microscopy
journal, November 2006


High Resolution 3D X-Ray Diffraction Microscopy
journal, August 2002


Towards an understanding of radiation damage in cryocooled macromolecular crystals
journal, April 2005


Experimental determination of the radiation dose limit for cryocooled protein crystals
journal, March 2006

  • Owen, R. L.; Rudino-Pinera, E.; Garman, E. F.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 13
  • DOI: 10.1073/pnas.0600973103

In vivo veritas: electron cryotomography of cells
journal, August 2002


Radiation damage in macromolecular cryocrystallography
journal, October 2006


Reconstruction of the Shapes of Gold Nanocrystals Using Coherent X-Ray Diffraction
journal, October 2001


Hard-X-Ray Lensless Imaging of Extended Objects
journal, January 2007


X-ray microscopy
journal, May 1995

  • Sayre, D.; Chapman, H. N.
  • Acta Crystallographica Section A Foundations of Crystallography, Vol. 51, Issue 3
  • DOI: 10.1107/S0108767394011803

Multiple reference Fourier transform holography with soft x rays
journal, October 2006

  • Schlotter, W. F.; Rick, R.; Chen, K.
  • Applied Physics Letters, Vol. 89, Issue 16
  • DOI: 10.1063/1.2364259

Cryo X-ray microscopy with high spatial resolution in amplitude and phase contrast
journal, November 1998


Biological imaging by soft x-ray diffraction microscopy
journal, October 2005

  • Shapiro, D.; Thibault, P.; Beetz, T.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 43
  • DOI: 10.1073/pnas.0503305102

Powder diffraction from a continuous microjet of submicrometer protein crystals
journal, October 2008

  • Shapiro, D. A.; Chapman, H. N.; DePonte, D.
  • Journal of Synchrotron Radiation, Vol. 15, Issue 6
  • DOI: 10.1107/S0909049508024151

Diffractive imaging of nonperiodic materials with future coherent X-ray sources
journal, August 2004

  • Shen, Qun; Bazarov, Ivan; Thibault, Pierre
  • Journal of Synchrotron Radiation, Vol. 11, Issue 5
  • DOI: 10.1107/S0909049504016772

How does Radiation Damage in Protein Crystals Depend on X-Ray Dose?
journal, January 2003


Single Molecule Diffraction
journal, May 2004


Dose, exposure time and resolution in serial X-ray crystallography
journal, December 2007


Three-Dimensional Imaging of Microstructure in Au Nanocrystals
journal, April 2003


Automated diffraction image analysis and spot searching for high-throughput crystal screening
journal, January 2006

  • Zhang, Zepu; Sauter, Nicholas K.; van den Bedem, Henry
  • Journal of Applied Crystallography, Vol. 39, Issue 1
  • DOI: 10.1107/S0021889805040677

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    Radiation damage studies in cardiac muscle cells and tissue using microfocused X-ray beams: experiment and simulation
    journal, June 2019

    • Nicolas, Jan-David; Aeffner, Sebastian; Salditt, Tim
    • Journal of Synchrotron Radiation, Vol. 26, Issue 4
    • DOI: 10.1107/s1600577519006817

    Simple convergent-nozzle aerosol injector for single-particle diffractive imaging with X-ray free-electron lasers
    journal, June 2015

    • Kirian, R. A.; Awel, S.; Eckerskorn, N.
    • Structural Dynamics, Vol. 2, Issue 4
    • DOI: 10.1063/1.4922648

    Propagation-based phase-contrast x-ray tomography of cochlea using a compact synchrotron source
    journal, March 2018


    Monte Carlo calculation of ion, electron, and photon spectra of xenon atoms in x-ray free-electron laser pulses
    journal, June 2012


    Resolution and dose dependence of radiation damage in biomolecular systems
    journal, September 2019


    Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell
    journal, November 2009


    Soft x-ray ptychography studies of nanoscale magnetic and structural correlations in thin SmCo5 films
    journal, February 2016

    • Shi, X.; Fischer, P.; Neu, V.
    • Applied Physics Letters, Vol. 108, Issue 9
    • DOI: 10.1063/1.4942776

    Cryogenic X-Ray Diffraction Microscopy for Biological Samples
    journal, November 2009


    In-situ Scanning Transmission X-Ray Microscopy of Catalytic Solids and Related Nanomaterials
    journal, March 2010

    • de Groot, Frank M. F.; de Smit, Emiel; van Schooneveld, Matti M.
    • ChemPhysChem, Vol. 11, Issue 5
    • DOI: 10.1002/cphc.200901023

    Perspective: Towards single shot time-resolved microscopy using short wavelength table-top light sources
    text, January 2019

    • Helk, T.; Zürch, M.; Spielmann, C.
    • GSI Helmholtzzentrum fuer Schwerionenforschung, GSI, Darmstadt
    • DOI: 10.15120/gsi-2019-00570

    Radiation damage in protein crystallography at X-ray free-electron lasers
    journal, January 2019


    Femtosecond diffractive imaging with a soft-X-ray free-electron laser
    journal, November 2006

    • Chapman, Henry N.; Barty, Anton; Bogan, Michael J.
    • Nature Physics, Vol. 2, Issue 12
    • DOI: 10.1038/nphys461

    Serial Femtosecond Crystallography
    text, January 2015


    Multimodal x-ray and electron microscopy of the Allende meteorite
    journal, September 2019


    XFELs for structure and dynamics in biology
    journal, May 2017


    Radiation-damage investigation of a DNA 16-mer
    journal, June 2019

    • Bugris, Valéria; Harmat, Veronika; Ferenc, Györgyi
    • Journal of Synchrotron Radiation, Vol. 26, Issue 4
    • DOI: 10.1107/s160057751900763x

    Chemical composition mapping with nanometre resolution by soft X-ray microscopy
    journal, September 2014


    Quantitative disentanglement of nanocrystalline phases in cement pastes by synchrotron ptychographic X-ray tomography
    journal, April 2019


    Low-dose three-dimensional hard x-ray imaging of bacterial cells
    text, January 2012

    • Bartels, M.; Priebe, M.; Wilke, Robin
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/phppubdb-23830

    Imaging single cells in a beam of live cyanobacteria with an X-ray laser
    journal, February 2015

    • van der Schot, Gijs; Svenda, Martin; Maia, Filipe R. N. C.
    • Nature Communications, Vol. 6, Issue 1
    • DOI: 10.1038/ncomms6704

    X-ray focusing with efficient high-NA multilayer Laue lenses
    journal, November 2017

    • Bajt, Saša; Prasciolu, Mauro; Fleckenstein, Holger
    • Light: Science & Applications, Vol. 7, Issue 3
    • DOI: 10.1038/lsa.2017.162

    Ptychographic X-ray computed tomography at the nanoscale
    journal, September 2010

    • Dierolf, Martin; Menzel, Andreas; Thibault, Pierre
    • Nature, Vol. 467, Issue 7314
    • DOI: 10.1038/nature09419

    Room-temperature macromolecular serial crystallography using synchrotron radiation
    journal, May 2014


    Three-dimensional imaging of integrated circuits with macro- to nanoscale zoom
    journal, October 2019

    • Holler, Mirko; Odstrcil, Michal; Guizar-Sicairos, Manuel
    • Nature Electronics, Vol. 2, Issue 10
    • DOI: 10.1038/s41928-019-0309-z

    The Bionanoprobe: hard X-ray fluorescence nanoprobe with cryogenic capabilities
    journal, December 2013


    A Monte Carlo ray-tracing simulation of coherent X-ray diffractive imaging
    journal, January 2020

    • Fevola, Giovanni; Bergbäck Knudsen, Erik; Ramos, Tiago
    • Journal of Synchrotron Radiation, Vol. 27, Issue 1
    • DOI: 10.1107/s1600577519014425

    Outrunning damage: Electrons vs X-rays—timescales and mechanisms
    journal, July 2017


    Outrun radiation damage with electrons?
    journal, March 2015


    Classification of projection images of proteins with structural polymorphism by manifold: A simulation study for x-ray free-electron laser diffraction imaging
    journal, September 2015


    Laboratory-Based Cryogenic Soft X-Ray Tomography with Correlative Cryo-Light and Electron Microscopy
    journal, January 2013

    • Carlson, David B.; Gelb, Jeff; Palshin, Vadim
    • Microscopy and Microanalysis, Vol. 19, Issue 1
    • DOI: 10.1017/s1431927612013827

    Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae
    journal, February 2015

    • Deng, Junjing; Vine, David J.; Chen, Si
    • Proceedings of the National Academy of Sciences, Vol. 112, Issue 8
    • DOI: 10.1073/pnas.1413003112

    Three-dimensional coherent diffractive imaging on non-periodic specimens at the ESRF beamline ID10
    journal, March 2014


    Impact of ultrafast electronic damage in single-particle x-ray imaging experiments
    journal, November 2012


    Hard X-rays as pump and probe of atomic motion in oxide glasses
    journal, June 2017


    Materials characterization by synchrotron x-ray microprobes and nanoprobes
    journal, June 2018


    Quantitative ptychographic bio-imaging in the water window
    text, January 2018

    • Rose, Max; Senkbeil, Tobias; Von Gundlach, Andreas
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2018-00468

    Low Dose X-Ray Speckle Visibility Spectroscopy Reveals Nanoscale Dynamics in Radiation Sensitive Ionic Liquids
    text, January 2018

    • Verwohlt, Jan; Reiser, Mario; Randolph, Lisa
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2018-03851

    Signal-to-noise, spatial resolution and information capacity of coherent diffraction imaging
    journal, September 2018


    Diffraction before destruction
    journal, July 2014

    • Chapman, Henry N.; Caleman, Carl; Timneanu, Nicusor
    • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, Issue 1647
    • DOI: 10.1098/rstb.2013.0313

    Electron tomography for functional nanomaterials
    journal, April 2020


    IDATEN and G-SITENNO : GUI-assisted software for coherent X-ray diffraction imaging experiments and data analyses at SACLA
    journal, October 2014

    • Sekiguchi, Yuki; Yamamoto, Masaki; Oroguchi, Tomotaka
    • Journal of Synchrotron Radiation, Vol. 21, Issue 6
    • DOI: 10.1107/s1600577514017111

    Correlative multiscale tomography of biological materials
    journal, July 2016


    Unsupervised classification of single-particle X-ray diffraction snapshots by spectral clustering
    journal, January 2011

    • Yoon, Chun Hong; Schwander, Peter; Abergel, Chantal
    • Optics Express, Vol. 19, Issue 17
    • DOI: 10.1364/oe.19.016542

    Effects of soft X-ray radiation damage on paraffin-embedded rat tissues supported on ultralene: a chemical perspective
    journal, April 2018

    • Bedolla, Diana E.; Mantuano, Andrea; Pickler, Arissa
    • Journal of Synchrotron Radiation, Vol. 25, Issue 3
    • DOI: 10.1107/s1600577518003235

    Macromolecular structures probed by combining single-shot free-electron laser diffraction with synchrotron coherent X-ray imaging
    journal, May 2014

    • Gallagher-Jones, Marcus; Bessho, Yoshitaka; Kim, Sunam
    • Nature Communications, Vol. 5, Issue 1
    • DOI: 10.1038/ncomms4798

    Hard X-ray imaging of bacterial cells: nano-diffraction and ptychographic reconstruction
    journal, January 2012


    Computed stereo lensless X-ray imaging
    journal, April 2019


    High-resolution chemical imaging of gold nanoparticles using hard x-ray ptychography
    journal, May 2013

    • Hoppe, R.; Reinhardt, J.; Hofmann, G.
    • Applied Physics Letters, Vol. 102, Issue 20
    • DOI: 10.1063/1.4807020

    Quantitative and correlative extreme ultraviolet coherent imaging of mouse hippocampal neurons at high resolution
    journal, May 2020

    • Baksh, Peter D.; Ostrčil, Michal; Miszczak, Magdalena
    • Science Advances, Vol. 6, Issue 18
    • DOI: 10.1126/sciadv.aaz3025

    Computational studies of x-ray scattering from three-dimensionally-aligned asymmetric-top molecules
    journal, April 2010


    Outrunning free radicals in room-temperature macromolecular crystallography
    journal, June 2012

    • Owen, Robin L.; Axford, Danny; Nettleship, Joanne E.
    • Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 7
    • DOI: 10.1107/s0907444912012553

    Low-dose x-ray tomography through a deep convolutional neural network
    journal, February 2018


    Semi-implicit relaxed Douglas-Rachford algorithm (sDR) for ptychography
    journal, January 2019

    • Pham, Minh; Rana, Arjun; Miao, Jianwei
    • Optics Express, Vol. 27, Issue 22
    • DOI: 10.1364/oe.27.031246

    Dose efficient Compton X-ray microscopy
    journal, January 2018


    Simulated sample heating from a nanofocused X-ray beam
    journal, August 2017


    Imaging Fully Hydrated Whole Cells by Coherent X-Ray Diffraction Microscopy
    journal, February 2013


    Specimen preparation for cryogenic coherent X-ray diffraction imaging of biological cells and cellular organelles by using the X-ray free-electron laser at SACLA
    journal, May 2016

    • Kobayashi, Amane; Sekiguchi, Yuki; Oroguchi, Tomotaka
    • Journal of Synchrotron Radiation, Vol. 23, Issue 4
    • DOI: 10.1107/s1600577516007736

    Transmission and emission x-ray microscopy: operation modes, contrast mechanisms and applications
    journal, February 2011

    • Kaulich, Burkhard; Thibault, Pierre; Gianoncelli, Alessandra
    • Journal of Physics: Condensed Matter, Vol. 23, Issue 8
    • DOI: 10.1088/0953-8984/23/8/083002

    Dose requirements for resolving a given feature in an object by coherent x-ray diffraction imaging
    journal, March 2010


    Influence of gas atmospheres and ceria on the stability of nanoporous gold studied by environmental electron microscopy and in situ ptychography
    journal, January 2016

    • Baier, Sina; Wittstock, Arne; Damsgaard, Christian D.
    • RSC Advances, Vol. 6, Issue 86
    • DOI: 10.1039/c6ra12853j

    Ptychographic Nano-Analytical Microscope (PtyNAMi) at PETRA III: signal-to-background optimization for imaging with high sensitivity
    conference, September 2019

    • Schroer, Christian G.; Seyrich, Martin; Schropp, Andreas
    • X-Ray Nanoimaging: Instruments and Methods IV
    • DOI: 10.1117/12.2529096

    Soft X-Ray Microscopy Radiation Damage On Fixed Cells Investigated With Synchrotron Radiation FTIR Microscopy
    journal, May 2015

    • Gianoncelli, A.; Vaccari, L.; Kourousias, G.
    • Scientific Reports, Vol. 5, Issue 1
    • DOI: 10.1038/srep10250

    Signal enhancement and Patterson-search phasing for high-spatial-resolution coherent X-ray diffraction imaging of biological objects
    journal, January 2015

    • Takayama, Yuki; Maki-Yonekura, Saori; Oroguchi, Tomotaka
    • Scientific Reports, Vol. 5, Issue 1
    • DOI: 10.1038/srep08074

    The 2018 correlative microscopy techniques roadmap
    journal, August 2018

    • Ando, Toshio; Bhamidimarri, Satya Prathyusha; Brending, Niklas
    • Journal of Physics D: Applied Physics, Vol. 51, Issue 44
    • DOI: 10.1088/1361-6463/aad055

    Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source
    journal, August 2016

    • Munke, Anna; Andreasson, Jakob; Aquila, Andrew
    • Scientific Data, Vol. 3, Issue 1
    • DOI: 10.1038/sdata.2016.64

    Quantitative biological imaging by ptychographic x-ray diffraction microscopy
    journal, December 2009

    • Giewekemeyer, K.; Thibault, P.; Kalbfleisch, S.
    • Proceedings of the National Academy of Sciences, Vol. 107, Issue 2
    • DOI: 10.1073/pnas.0905846107

    Radiation damage in small-molecule crystallography: fact not fiction
    journal, June 2019


    Signal-to-noise and radiation exposure considerations in conventional and diffraction x-ray microscopy
    journal, January 2009

    • Huang, Xiaojing; Miao, Huijie; Steinbrener, Jan
    • Optics Express, Vol. 17, Issue 16
    • DOI: 10.1364/oe.17.013541

    Nanostructure surveys of macroscopic specimens by small-angle scattering tensor tomography
    journal, November 2015

    • Liebi, Marianne; Georgiadis, Marios; Menzel, Andreas
    • Nature, Vol. 527, Issue 7578
    • DOI: 10.1038/nature16056

    OMNY—A tOMography Nano crYo stage
    journal, April 2018

    • Holler, M.; Raabe, J.; Diaz, A.
    • Review of Scientific Instruments, Vol. 89, Issue 4
    • DOI: 10.1063/1.5020247

    Photon-limited ptychography of 3D objects via Bayesian reconstruction
    journal, January 2019


    Multiwavelength Anomalous Diffraction at High X-Ray Intensity
    text, January 2011

    • Son, Sang-Kil; Chapman, Henry N.; Santra, Robin
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/phppubdb-17977

    Coherent X-Ray Diffraction Imaging of Chloroplasts from Cyanidioschyzon merolae by Using X-Ray Free Electron Laser
    journal, March 2015

    • Takayama, Yuki; Inui, Yayoi; Sekiguchi, Yuki
    • Plant and Cell Physiology, Vol. 56, Issue 7
    • DOI: 10.1093/pcp/pcv032

    Quantitative 3D imaging of whole, unstained cells by using X-ray diffraction microscopy
    journal, June 2010

    • Jiang, H.; Song, C.; Chen, C. -C.
    • Proceedings of the National Academy of Sciences, Vol. 107, Issue 25
    • DOI: 10.1073/pnas.1000156107

    The fluence–resolution relationship in holographic and coherent diffractive imaging
    journal, March 2017


    X-ray lasers for structural and dynamic biology
    journal, September 2012


    A comparison of absorption and phase contrast for X-ray imaging of biological cells
    journal, August 2018


    Opportunities in multidimensional trace metal imaging: taking copper-associated disease research to the next level
    journal, October 2012


    Three-dimensional magnetization structures revealed with X-ray vector nanotomography
    journal, July 2017

    • Donnelly, Claire; Guizar-Sicairos, Manuel; Scagnoli, Valerio
    • Nature, Vol. 547, Issue 7663
    • DOI: 10.1038/nature23006

    Lifetimes and spatio-temporal response of protein crystals in intense X-ray microbeams
    journal, October 2017


    Resolution enhancement in coherent x-ray diffraction imaging by overcoming instrumental noise
    journal, January 2014

    • Kim, Chan; Kim, Yoonhee; Song, Changyong
    • Optics Express, Vol. 22, Issue 23
    • DOI: 10.1364/oe.22.029161

    Ab initio nonrigid X-ray nanotomography
    text, January 2019

    • Odstrcil, Michal; Holler, Mirko; Raabe, Jörg
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2020-00848

    Ptychography with broad-bandwidth radiation
    journal, April 2014

    • Enders, B.; Dierolf, M.; Cloetens, P.
    • Applied Physics Letters, Vol. 104, Issue 17
    • DOI: 10.1063/1.4874304

    Quantitative study of contrast enhancement in soft X-ray micrographs of insect eyes by tissue selective mass loss
    journal, July 2014

    • Späth, Andreas; Watts, Benjamin; Wasserthal, Lutz Thilo
    • Journal of Synchrotron Radiation, Vol. 21, Issue 5
    • DOI: 10.1107/s1600577514013940

    X-ray ptychographic computed tomography at 16 nm isotropic 3D resolution
    journal, January 2014

    • Holler, M.; Diaz, A.; Guizar-Sicairos, M.
    • Scientific Reports, Vol. 4, Issue 1
    • DOI: 10.1038/srep03857

    Diffraction apparatus and procedure in tomography X-ray diffraction imaging for biological cells at cryogenic temperature using synchrotron X-ray radiation
    journal, October 2018

    • Kobayashi, Amane; Takayama, Yuki; Okajima, Koji
    • Journal of Synchrotron Radiation, Vol. 25, Issue 6
    • DOI: 10.1107/s1600577518012687

    Observation of femtosecond X-ray interactions with matter using an X-ray–X-ray pump–probe scheme
    journal, January 2016

    • Inoue, Ichiro; Inubushi, Yuichi; Sato, Takahiro
    • Proceedings of the National Academy of Sciences, Vol. 113, Issue 6
    • DOI: 10.1073/pnas.1516426113

    High-resolution x-ray diffraction microscopy of specifically labeled yeast cells
    journal, April 2010

    • Nelson, J.; Huang, X.; Steinbrener, J.
    • Proceedings of the National Academy of Sciences, Vol. 107, Issue 16
    • DOI: 10.1073/pnas.0910874107

    Femtosecond protein nanocrystallography—data analysis methods
    journal, January 2010

    • Kirian, Richard A.; Wang, Xiaoyu; Weierstall, Uwe
    • Optics Express, Vol. 18, Issue 6
    • DOI: 10.1364/oe.18.005713

    Coherent lensless X-ray imaging
    journal, November 2010


    X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning
    journal, March 2017


    Where is it and how much? Mapping and quantifying elements in single cells
    journal, January 2016

    • Malucelli, Emil; Fratini, Michela; Notargiacomo, Andrea
    • The Analyst, Vol. 141, Issue 18
    • DOI: 10.1039/c6an01091a

    Cryo scanning transmission x-ray microscope optimized for spectrotomography
    journal, September 2018

    • Leontowich, A. F. G.; Berg, R.; Regier, C. N.
    • Review of Scientific Instruments, Vol. 89, Issue 9
    • DOI: 10.1063/1.5041009

    Atomic structure of granulin determined from native nanocrystalline granulovirus using an X-ray free-electron laser
    journal, February 2017

    • Gati, Cornelius; Oberthuer, Dominik; Yefanov, Oleksandr
    • Proceedings of the National Academy of Sciences, Vol. 114, Issue 9
    • DOI: 10.1073/pnas.1609243114

    Radiation Damage in Macromolecular Crystallography
    journal, November 2015


    The Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS)
    journal, March 2010


    Multiwavelength Anomalous Diffraction at High X-Ray Intensity
    journal, November 2011


    The minimum crystal size needed for a complete diffraction data set
    journal, March 2010

    • Holton, James M.; Frankel, Kenneth A.
    • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
    • DOI: 10.1107/S0907444910007262

    Temperature-dependent radiation sensitivity and order of 70S ribosome crystals
    journal, October 2014

    • Warkentin, Matthew; Hopkins, Jesse B.; Haber, Jonah B.
    • Acta Crystallographica Section D Biological Crystallography, Vol. 70, Issue 11
    • DOI: 10.1107/s1399004714017672

    Use of small-angle X-ray scattering to resolve intracellular structure changes of Escherichia coli cells induced by antibiotic treatment
    journal, December 2016

    • von Gundlach, A. R.; Garamus, V. M.; Willey, T. M.
    • Journal of Applied Crystallography, Vol. 49, Issue 6
    • DOI: 10.1107/s1600576716018562

    Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells
    journal, August 2015


    Radiation damage in small-molecule crystallography: fact not fiction
    text, January 2019


    Robust contrast-transfer-function phase retrieval via flexible deep learning networks
    journal, January 2019


    Hard x-ray multi-projection imaging for single-shot approaches
    journal, January 2018


    Quantitative and correlative extreme ultraviolet coherent imaging of mouse hippocampal neurons at high resolution
    text, January 2020


    The 2018 correlative microscopy techniques roadmap
    collection, January 2018

    • Ando, Toshio; Bhamidimarri, Satya Prathyusha; Brending, Niklas
    • Universität des Saarlandes
    • DOI: 10.22028/d291-29241

    Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source
    text, January 2016

    • Munke, Anna; Andreasson, Jakob; Aquila, Andrew
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2016-02771

    Influence of gas atmospheres and ceria on the stability of nanoporous gold studied by environmental electron microscopy and in situ ptychography
    text, January 2016

    • Baier, Sina; Wittstock, Arne; Damsgaard, Christian D.
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2016-05565

    X-ray focusing with efficient high-NA multilayer Laue lenses
    text, January 2017

    • Bajt, Saša; Prasciolu, Mauro; Fleckenstein, Holger
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2017-12451

    Computed stereo lensless X-ray imaging
    text, January 2019


    Radiation damage studies in cardiac muscle cells and tissue using microfocused X-ray beams: experiment and simulation
    text, January 2019

    • Nicolas, Jan-David; Aeffner, Sebastian; Salditt, Tim
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2020-00523