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Title: Cryo diffraction microscopy: Ice conditions and finite supports

Abstract

Using a signal-to-noise ratio estimation based on correlations between multiple simulated images, we compare the dose efficiency of two soft x-ray imaging systems: incoherent brightfield imaging using zone plate optics in a transmission x-ray microscope (TXM), and x-ray diffraction microscopy (XDM) where an image is reconstructed from the far-field coherent diffraction pattern. In XDM one must computationally phase weak diffraction signals; in TXM one suffers signal losses due to the finite numerical aperture and efficiency of the optics. In simulations with objects representing isolated cells such as yeast, we find that XDM has the potential for delivering equivalent resolution images using fewer photons. This can be an important advantage for studying radiation-sensitive biological and soft matter specimens.

Authors:
 [1];  [2];  [1];  [2];  [2];  [1];  [2];  [1];  [1];  [1]
  1. Stony Brook Univ., Stony Brook, NY (United States)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Stony Brook Univ., Stony Brook, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1164147
Grant/Contract Number:  
FG02-04ER46128
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Physics. Conference Series (Online)
Additional Journal Information:
Journal Name: Journal of Physics. Conference Series (Online); Journal Volume: 186; Journal ID: ISSN 1742-6596
Publisher:
Institute of Physics (IOP)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Miao, H., Downing, K., Huang, X., Kirz, J., Marchesini, S., Nelson, J., Shapiro, D., Steinbrener, J., Stewart, A., and Jacobsen, C. Cryo diffraction microscopy: Ice conditions and finite supports. United States: N. p., 2009. Web. doi:10.1088/1742-6596/186/1/012055.
Miao, H., Downing, K., Huang, X., Kirz, J., Marchesini, S., Nelson, J., Shapiro, D., Steinbrener, J., Stewart, A., & Jacobsen, C. Cryo diffraction microscopy: Ice conditions and finite supports. United States. doi:10.1088/1742-6596/186/1/012055.
Miao, H., Downing, K., Huang, X., Kirz, J., Marchesini, S., Nelson, J., Shapiro, D., Steinbrener, J., Stewart, A., and Jacobsen, C. Fri . "Cryo diffraction microscopy: Ice conditions and finite supports". United States. doi:10.1088/1742-6596/186/1/012055. https://www.osti.gov/servlets/purl/1164147.
@article{osti_1164147,
title = {Cryo diffraction microscopy: Ice conditions and finite supports},
author = {Miao, H. and Downing, K. and Huang, X. and Kirz, J. and Marchesini, S. and Nelson, J. and Shapiro, D. and Steinbrener, J. and Stewart, A. and Jacobsen, C.},
abstractNote = {Using a signal-to-noise ratio estimation based on correlations between multiple simulated images, we compare the dose efficiency of two soft x-ray imaging systems: incoherent brightfield imaging using zone plate optics in a transmission x-ray microscope (TXM), and x-ray diffraction microscopy (XDM) where an image is reconstructed from the far-field coherent diffraction pattern. In XDM one must computationally phase weak diffraction signals; in TXM one suffers signal losses due to the finite numerical aperture and efficiency of the optics. In simulations with objects representing isolated cells such as yeast, we find that XDM has the potential for delivering equivalent resolution images using fewer photons. This can be an important advantage for studying radiation-sensitive biological and soft matter specimens.},
doi = {10.1088/1742-6596/186/1/012055},
journal = {Journal of Physics. Conference Series (Online)},
number = ,
volume = 186,
place = {United States},
year = {2009},
month = {9}
}

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