Biological imaging by soft x-ray diffraction microscopy

doi:10.1073/pnas.0503305102

Title: Biological imaging by soft x-ray diffraction microscopy

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Abstract

We have used the method of x-ray diffraction microscopy to image the complex-valued exit wave of an intact and unstained yeast cell. The images of the freeze-dried cell, obtained by using 750-eV x-rays from different angular orientations, portray several of the cell's major internal components to 30-nm resolution. The good agreement among the independently recovered structures demonstrates the accuracy of the imaging technique. To obtain the best possible reconstructions, we have implemented procedures for handling noisy and incomplete diffraction data, and we propose a method for determining the reconstructed resolution. This work represents a previously uncharacterized application of x-ray diffraction microscopy to a specimen of this complexity and provides confidence in the feasibility of the ultimate goal of imaging biological specimens at 10-nm resolution in three dimensions.

Authors:

Shapiro, D. ^[1]; Thibault, P. ^[2]; Beetz, T. ^[3]; Elser, V. ^[2]; Howells, M. ^[4]; Jacobsen, C. ^[3]; Kirz, J. ^[5]; Lima, E. ^[1]; Miao, H. ^[1]; Neiman, A. M. ^[6]; Sayre, D. ^[1]

Stony Brook Univ., Stony Brook, NY (United States)
Cornell Univ., Ithaca, NY (United States)
Stony Brook Univ., Stony Brook, NY (United States); Brookhaven National Lab., Upton, NY (United States). Center for Functional Nanomaterials
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Advanced Light Source
Stony Brook Univ., Stony Brook, NY (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Advanced Light Source
State Univ. of New York at Stony Brook, NY (United States)

Publication Date:: 2005-10-25

Research Org.:: Stony Brook Univ., Stony Brook, NY (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

OSTI Identifier:: 1164139

Grant/Contract Number:: FG02-04ER46128

Resource Type:: Accepted Manuscript

Journal Name:: Proceedings of the National Academy of Sciences of the United States of America

Additional Journal Information:: Journal Volume: 102; Journal Issue: 43; Journal ID: ISSN 0027-8424

Publisher:: National Academy of Sciences, Washington, DC (United States)

Country of Publication:: United States

Language:: English

Subject:: 36 MATERIALS SCIENCE; 77 NANOSCIENCE AND NANOTECHNOLOGY

Citation Formats


                    Shapiro, D., Thibault, P., Beetz, T., Elser, V., Howells, M., Jacobsen, C., Kirz, J., Lima, E., Miao, H., Neiman, A. M., and Sayre, D. Biological imaging by soft x-ray diffraction microscopy.  United States: N. p., 2005. 
        Web.  doi:10.1073/pnas.0503305102.

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                    Shapiro, D., Thibault, P., Beetz, T., Elser, V., Howells, M., Jacobsen, C., Kirz, J., Lima, E., Miao, H., Neiman, A. M., & Sayre, D. Biological imaging by soft x-ray diffraction microscopy.  United States.  doi:10.1073/pnas.0503305102.

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                    Shapiro, D., Thibault, P., Beetz, T., Elser, V., Howells, M., Jacobsen, C., Kirz, J., Lima, E., Miao, H., Neiman, A. M., and Sayre, D. Tue .  
        "Biological imaging by soft x-ray diffraction microscopy".  United States.  doi:10.1073/pnas.0503305102.  https://www.osti.gov/servlets/purl/1164139.

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@article{osti_1164139,

  title        = {Biological imaging by soft x-ray diffraction microscopy},

  author       = {Shapiro, D. and Thibault, P. and Beetz, T. and Elser, V. and Howells, M. and Jacobsen, C. and Kirz, J. and Lima, E. and Miao, H. and Neiman, A. M. and Sayre, D.},

  abstractNote = {We have used the method of x-ray diffraction microscopy to image the complex-valued exit wave of an intact and unstained yeast cell. The images of the freeze-dried cell, obtained by using 750-eV x-rays from different angular orientations, portray several of the cell's major internal components to 30-nm resolution. The good agreement among the independently recovered structures demonstrates the accuracy of the imaging technique. To obtain the best possible reconstructions, we have implemented procedures for handling noisy and incomplete diffraction data, and we propose a method for determining the reconstructed resolution. This work represents a previously uncharacterized application of x-ray diffraction microscopy to a specimen of this complexity and provides confidence in the feasibility of the ultimate goal of imaging biological specimens at 10-nm resolution in three dimensions.},

  doi          = {10.1073/pnas.0503305102},

  journal      = {Proceedings of the National Academy of Sciences of the United States of America},

  number       = 43,

  volume       = 102,

  place        = {United States},

  year         = {2005},

  month        = {10}

}

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DOI: 10.1073/pnas.0503305102

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