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Title: Preservation of H 2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings

Abstract

To assess the applicability of latex cell coatings as an ‘off-the-shelf’ biocatalyst, the effect of osmoprotectants, temperature, humidity and O2 on preservation of H2 production in Rhodopseudomonas palustris coatings was evaluated. Immediately following latex coating coalescence (24 h) and for up to 2 weeks of dry storage, rehydrated coatings containing different osmoprotectants displayed similar rates of H2 production. Beyond 2 weeks of storage, sorbitol-treated coatings lost all H2 production activity, whereas considerable H2 production was still detected in sucrose- and trehalose-stabilized coatings. We stored the coatings at a relative humidity level which significantly impacts the recovery and subsequent rates of H2 production. After 4 weeks storage under air at 60% humidity, coatings produced only trace amounts of H2 (0–0.1% headspace accumulation), whereas those stored at < 5% humidity retained 27–53% of their H2 production activity after 8 weeks of storage. Furthermore, when stored in argon at < 5% humidity and room temperature, R. palustris coatings retained full H2 production activity for 3 months, implicating oxidative damage as a key factor limiting coating storage. Ultimately, the results demonstrate that biocatalytic latex coatings are an attractive cell immobilization platform for preservation of bioactivity in the dry state.

Authors:
 [1];  [2];  [3];  [2];  [3];  [1];  [2]
  1. Univ. of South Carolina, Aiken, SC (United States)
  2. Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)
  3. North Carolina State Univ., Raleigh, NC (United States)
Publication Date:
Research Org.:
Univ. of South Carolina, Columbia, SC (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B); USDOE Office of Environmental Management (EM)
OSTI Identifier:
1163478
Report Number(s):
DOE-USCA-3152-2
Journal ID: ISSN 1751-7915
Grant/Contract Number:  
EE0003152
Resource Type:
Accepted Manuscript
Journal Name:
Microbial Biotechnology (Online)
Additional Journal Information:
Journal Name: Microbial Biotechnology (Online); Journal Volume: 6; Journal Issue: 5; Journal ID: ISSN 1751-7915
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Piskorska, M., Soule, T., Gosse, J. L., Milliken, C., Flickinger, M. C., Smith, G. W., and Yeager, C. M. Preservation of H 2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings. United States: N. p., 2013. Web. doi:10.1111/1751-7915.12032.
Piskorska, M., Soule, T., Gosse, J. L., Milliken, C., Flickinger, M. C., Smith, G. W., & Yeager, C. M. Preservation of H 2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings. United States. doi:10.1111/1751-7915.12032.
Piskorska, M., Soule, T., Gosse, J. L., Milliken, C., Flickinger, M. C., Smith, G. W., and Yeager, C. M. Sun . "Preservation of H 2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings". United States. doi:10.1111/1751-7915.12032. https://www.osti.gov/servlets/purl/1163478.
@article{osti_1163478,
title = {Preservation of H 2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings},
author = {Piskorska, M. and Soule, T. and Gosse, J. L. and Milliken, C. and Flickinger, M. C. and Smith, G. W. and Yeager, C. M.},
abstractNote = {To assess the applicability of latex cell coatings as an ‘off-the-shelf’ biocatalyst, the effect of osmoprotectants, temperature, humidity and O2 on preservation of H2 production in Rhodopseudomonas palustris coatings was evaluated. Immediately following latex coating coalescence (24 h) and for up to 2 weeks of dry storage, rehydrated coatings containing different osmoprotectants displayed similar rates of H2 production. Beyond 2 weeks of storage, sorbitol-treated coatings lost all H2 production activity, whereas considerable H2 production was still detected in sucrose- and trehalose-stabilized coatings. We stored the coatings at a relative humidity level which significantly impacts the recovery and subsequent rates of H2 production. After 4 weeks storage under air at 60% humidity, coatings produced only trace amounts of H2 (0–0.1% headspace accumulation), whereas those stored at < 5% humidity retained 27–53% of their H2 production activity after 8 weeks of storage. Furthermore, when stored in argon at < 5% humidity and room temperature, R. palustris coatings retained full H2 production activity for 3 months, implicating oxidative damage as a key factor limiting coating storage. Ultimately, the results demonstrate that biocatalytic latex coatings are an attractive cell immobilization platform for preservation of bioactivity in the dry state.},
doi = {10.1111/1751-7915.12032},
journal = {Microbial Biotechnology (Online)},
number = 5,
volume = 6,
place = {United States},
year = {2013},
month = {7}
}

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