Preservation of H 2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings
Abstract
To assess the applicability of latex cell coatings as an ‘off-the-shelf’ biocatalyst, the effect of osmoprotectants, temperature, humidity and O2 on preservation of H2 production in Rhodopseudomonas palustris coatings was evaluated. Immediately following latex coating coalescence (24 h) and for up to 2 weeks of dry storage, rehydrated coatings containing different osmoprotectants displayed similar rates of H2 production. Beyond 2 weeks of storage, sorbitol-treated coatings lost all H2 production activity, whereas considerable H2 production was still detected in sucrose- and trehalose-stabilized coatings. We stored the coatings at a relative humidity level which significantly impacts the recovery and subsequent rates of H2 production. After 4 weeks storage under air at 60% humidity, coatings produced only trace amounts of H2 (0–0.1% headspace accumulation), whereas those stored at < 5% humidity retained 27–53% of their H2 production activity after 8 weeks of storage. Furthermore, when stored in argon at < 5% humidity and room temperature, R. palustris coatings retained full H2 production activity for 3 months, implicating oxidative damage as a key factor limiting coating storage. Ultimately, the results demonstrate that biocatalytic latex coatings are an attractive cell immobilization platform for preservation of bioactivity in the dry state.
- Authors:
-
- Univ. of South Carolina, Aiken, SC (United States)
- Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)
- North Carolina State Univ., Raleigh, NC (United States)
- Publication Date:
- Research Org.:
- Univ. of South Carolina, Columbia, SC (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office; USDOE Office of Environmental Management (EM)
- OSTI Identifier:
- 1163478
- Report Number(s):
- DOE-USCA-3152-2
Journal ID: ISSN 1751-7915
- Grant/Contract Number:
- EE0003152
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Microbial Biotechnology (Online)
- Additional Journal Information:
- Journal Name: Microbial Biotechnology (Online); Journal Volume: 6; Journal Issue: 5; Journal ID: ISSN 1751-7915
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS
Citation Formats
Piskorska, M., Soule, T., Gosse, J. L., Milliken, C., Flickinger, M. C., Smith, G. W., and Yeager, C. M. Preservation of H 2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings. United States: N. p., 2013.
Web. doi:10.1111/1751-7915.12032.
Piskorska, M., Soule, T., Gosse, J. L., Milliken, C., Flickinger, M. C., Smith, G. W., & Yeager, C. M. Preservation of H 2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings. United States. https://doi.org/10.1111/1751-7915.12032
Piskorska, M., Soule, T., Gosse, J. L., Milliken, C., Flickinger, M. C., Smith, G. W., and Yeager, C. M. Sun .
"Preservation of H 2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings". United States. https://doi.org/10.1111/1751-7915.12032. https://www.osti.gov/servlets/purl/1163478.
@article{osti_1163478,
title = {Preservation of H 2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings},
author = {Piskorska, M. and Soule, T. and Gosse, J. L. and Milliken, C. and Flickinger, M. C. and Smith, G. W. and Yeager, C. M.},
abstractNote = {To assess the applicability of latex cell coatings as an ‘off-the-shelf’ biocatalyst, the effect of osmoprotectants, temperature, humidity and O2 on preservation of H2 production in Rhodopseudomonas palustris coatings was evaluated. Immediately following latex coating coalescence (24 h) and for up to 2 weeks of dry storage, rehydrated coatings containing different osmoprotectants displayed similar rates of H2 production. Beyond 2 weeks of storage, sorbitol-treated coatings lost all H2 production activity, whereas considerable H2 production was still detected in sucrose- and trehalose-stabilized coatings. We stored the coatings at a relative humidity level which significantly impacts the recovery and subsequent rates of H2 production. After 4 weeks storage under air at 60% humidity, coatings produced only trace amounts of H2 (0–0.1% headspace accumulation), whereas those stored at < 5% humidity retained 27–53% of their H2 production activity after 8 weeks of storage. Furthermore, when stored in argon at < 5% humidity and room temperature, R. palustris coatings retained full H2 production activity for 3 months, implicating oxidative damage as a key factor limiting coating storage. Ultimately, the results demonstrate that biocatalytic latex coatings are an attractive cell immobilization platform for preservation of bioactivity in the dry state.},
doi = {10.1111/1751-7915.12032},
journal = {Microbial Biotechnology (Online)},
number = 5,
volume = 6,
place = {United States},
year = {Sun Jul 21 00:00:00 EDT 2013},
month = {Sun Jul 21 00:00:00 EDT 2013}
}
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Works referenced in this record:
Role of Water in Particle Deformation and Compaction in Latex Film Formation
journal, August 1994
- Sperry, P. R.; Snyder, B. S.; O'Dowd, M. L.
- Langmuir, Vol. 10, Issue 8
Free-Radical Formation during Storage of Freeze-dried Serratia marcescens
journal, November 1961
- Dimmick, R. L.; Heckly, R. J.; Hollis, D. P.
- Nature, Vol. 192, Issue 4804
Protective effect of sorbitol and monosodium glutamate during storage of freeze-dried lactic acid bacteria
journal, May 2003
- Carvalho, Ana S.; Silva, Joana; Ho, Peter
- Le Lait, Vol. 83, Issue 3
Hydrogen Production by Photoreactive Nanoporous Latex Coatings of Nongrowing Rhodopseudomonas palustris CGA009
journal, February 2007
- Gosse, J. L.; Engel, B. J.; Rey, F. E.
- Biotechnology Progress, Vol. 23, Issue 1
Anhydrobiotics: The challenges of drying probiotic cultures
journal, February 2008
- Meng, X. C.; Stanton, C.; Fitzgerald, G. F.
- Food Chemistry, Vol. 106, Issue 4
Biological activity and mechanical stability of sol–gel-based biofilters using the freeze-gelation technique for immobilization of Rhodococcus ruber
journal, July 2011
- Pannier, Angela; Mkandawire, Martin; Soltmann, Ulrich
- Applied Microbiology and Biotechnology, Vol. 93, Issue 4
Functional Genomic Analysis of Three Nitrogenase Isozymes in the Photosynthetic Bacterium Rhodopseudomonas palustris
journal, November 2005
- Oda, Y.; Samanta, S. K.; Rey, F. E.
- Journal of Bacteriology, Vol. 187, Issue 22
The molecular nature of damage by oxygen to freeze-dried Escherichia coli
journal, February 1975
- Israeli, E.; Kohn, A.; Gitelman, Janina
- Cryobiology, Vol. 12, Issue 1
Microstructure development in drying latex coatings
journal, January 2005
- Ma, Yue; Davis, H. T.; Scriven, L. E.
- Progress in Organic Coatings, Vol. 52, Issue 1
Painting and Printing Living Bacteria: Engineering Nanoporous Biocatalytic Coatings to Preserve Microbial Viability and Intensify Reactivity
journal, February 2007
- Flickinger, M. C.; Schottel, J. L.; Bond, D. R.
- Biotechnology Progress, Vol. 23, Issue 1
A single-use luciferase-based mercury biosensor using Escherichia coli HB101 immobilized in a latex copolymer film
journal, July 1999
- Lyngberg, O. K.; Stemke, D. J.; Schottel, J. L.
- Journal of Industrial Microbiology and Biotechnology, Vol. 23, Issue 1
Development of a Set of Simple Bacterial Biosensors for Quantitative and Rapid Measurements of Arsenite and Arsenate in Potable Water
journal, October 2003
- Stocker, Judith; Balluch, Denisa; Gsell, Monika
- Environmental Science & Technology, Vol. 37, Issue 20
Freeze-drying of sol–gel encapsulated recombinant bioluminescent E. coli by using lyo-protectants
journal, February 2006
- Tessema, Dejene A.; Rosen, Rachel; Pedazur, Rami
- Sensors and Actuators B: Chemical, Vol. 113, Issue 2
Thermoprotective effect of sorbitol on proteins during dehydration
journal, February 1993
- Yoo, B.; Lee, C. M.
- Journal of Agricultural and Food Chemistry, Vol. 41, Issue 2
Production of Hydrogen Gas from Light and the Inorganic Electron Donor Thiosulfate by Rhodopseudomonas palustris
journal, October 2010
- Huang, J. J.; Heiniger, E. K.; McKinlay, J. B.
- Applied and Environmental Microbiology, Vol. 76, Issue 23
Immobilized viable microbial cells: from the process to the proteome… or the cart before the horse
journal, November 2004
- Junter, Guy-Alain; Jouenne, Thierry
- Biotechnology Advances, Vol. 22, Issue 8
Photobiological hydrogen production: photochemical efficiency and bioreactor design
journal, November 2002
- Akkerman, I.
- International Journal of Hydrogen Energy, Vol. 27, Issue 11-12
Photobiological production of hydrogen gas as a biofuel
journal, June 2010
- McKinlay, James B.; Harwood, Caroline S.
- Current Opinion in Biotechnology, Vol. 21, Issue 3
Survival of freeze-dried bacteria
journal, January 2008
- Miyamoto-Shinohara, Yukie; Sukenobe, Junji; Imaizumi, Takashi
- The Journal of General and Applied Microbiology, Vol. 54, Issue 1
Responses of Rhizobia to Desiccation in Relation to Osmotic Stress, Oxygen, and Temperature
journal, March 2007
- Vriezen, J. A. C.; de Bruijn, F. J.; Nusslein, K.
- Applied and Environmental Microbiology, Vol. 73, Issue 11
Staying alive: new perspectives on cell immobilization for biosensing purposes
journal, September 2011
- Michelini, Elisa; Roda, Aldo
- Analytical and Bioanalytical Chemistry, Vol. 402, Issue 5
Regulation of Uptake Hydrogenase and Effects of Hydrogen Utilization on Gene Expression in Rhodopseudomonas palustris
journal, August 2006
- Rey, F. E.; Oda, Y.; Harwood, C. S.
- Journal of Bacteriology, Vol. 188, Issue 17
Rapid in situ assessmetn of physiological activities in bacterial biofilms using fluorescent probes
journal, June 1994
- Yu, Feipeing P.; McFeters, Gordon A.
- Journal of Microbiological Methods, Vol. 20, Issue 1
Advances in preservation methods: keeping biosensor microorganisms alive and active
journal, February 2006
- Bjerketorp, Joakim; Håkansson, Sebastian; Belkin, Shimshon
- Current Opinion in Biotechnology, Vol. 17, Issue 1
Reconciling the incompatible: N2 fixation And O2
journal, December 1992
- Gallon, J. R.
- New Phytologist, Vol. 122, Issue 4
Preservation of micro-organisms by drying; A review
journal, August 2006
- Morgan, C. A.; Herman, N.; White, P. A.
- Journal of Microbiological Methods, Vol. 66, Issue 2
Immobilized cells: a review of recent literature
journal, February 1987
- Scott, Charles D.
- Enzyme and Microbial Technology, Vol. 9, Issue 2
Engineering the Microstructure and Permeability of Thin Multilayer Latex Biocatalytic Coatings Containing E. coli
journal, December 2001
- Lyngberg, O. K.; Ng, C. P.; Thiagarajan, V.
- Biotechnology Progress, Vol. 17, Issue 6
Paper Strip Whole Cell Biosensors: A Portable Test for the Semiquantitative Detection of Bacterial Quorum Signaling Molecules
journal, June 2010
- Struss, Anjali; Pasini, Patrizia; Ensor, C. Mark
- Analytical Chemistry, Vol. 82, Issue 11
Survival kinetics of lactic acid starter cultures during and after freeze drying
journal, September 1987
- Bozoǧlu, T. F.; Özilgen, M.; Bakir, U.
- Enzyme and Microbial Technology, Vol. 9, Issue 9
Cultivation of Green Algae Chlorella sp. in Different Wastewaters from Municipal Wastewater Treatment Plant
journal, November 2009
- Wang, Liang; Min, Min; Li, Yecong
- Applied Biochemistry and Biotechnology, Vol. 162, Issue 4
Encapsulation of cells within silica matrixes: Towards a new advance in the conception of living hybrid materials
journal, February 2010
- Meunier, Christophe F.; Dandoy, Philippe; Su, Bao-Lian
- Journal of Colloid and Interface Science, Vol. 342, Issue 2, p. 211-224
Hydrogen production during stationary phase in purple photosynthetic bacteria
journal, November 2008
- Melnicki, M.; Bianchi, L.; Dephilippis, R.
- International Journal of Hydrogen Energy, Vol. 33, Issue 22
Utilization of sol–gel ceramics for the immobilization of living microorganisms
journal, May 2008
- Soltmann, U.; Böttcher, H.
- Journal of Sol-Gel Science and Technology, Vol. 48, Issue 1-2
Permeability and reactivity of Thermotoga maritima in latex bimodal blend coatings at 80°C: a model high temperature biocatalytic coating
journal, March 2005
- Lyngberg, Olav K.; Solheid, Chris; Charaniya, Salim
- Extremophiles, Vol. 9, Issue 3
A model system for increasing the intensity of whole-cell biocatalysis: Investigation of the rate of oxidation ofD-sorbitol toL-sorbose by thin bi-layer latex coatings of non-growingGluconobacter oxydans
journal, January 2006
- Fidaleo, M.; Charaniya, S.; Solheid, C.
- Biotechnology and Bioengineering, Vol. 95, Issue 3
Membrane phase behavior of Escherichia coli during desiccation, rehydration, and growth recovery
journal, November 2009
- Scherber, Cally M.; Schottel, Janet L.; Aksan, Alptekin
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1788, Issue 11
Optimization of preservation conditions of As (III) bioreporter bacteria
journal, February 2009
- Kuppardt, Anke; Chatzinotas, Antonis; Breuer, Uta
- Applied Microbiology and Biotechnology, Vol. 82, Issue 4
Electrochemical characterization ofGeobacter sulfurreducens cells immobilized on graphite paper electrodes
journal, January 2008
- Srikanth, Shweta; Marsili, Enrico; Flickinger, Michael C.
- Biotechnology and Bioengineering, Vol. 99, Issue 5
Survival Rate of Microbes after Freeze-Drying and Long-Term Storage
journal, November 2000
- Miyamoto-Shinohara, Yukie; Imaizumi, Takashi; Sukenobe, Junji
- Cryobiology, Vol. 41, Issue 3
Some Factors Affecting the Viability of Dried Bacteria During Storage In Vacuo
journal, January 1974
- Marshall, Betty J.; Coote, G. G.; Scott, W. J.
- Applied Microbiology, Vol. 27, Issue 4
Protective Effect of Adonitol on Lactic Acid Bacteria Subjected to Freeze-Drying
journal, January 1983
- de Valdéz, Graciela F.; de Giori, Graciela S.; de Ruiz Holgado, Aída A. P.
- Applied and Environmental Microbiology, Vol. 45, Issue 1
A model system for increasing the intensity of whole-cell biocatalysis: Investigation of the rate of oxidation ofD-sorbitol toL-sorbose by thin bi-layer latex coatings of non-growingGluconobacter oxydans
journal, January 2006
- Fidaleo, M.; Charaniya, S.; Solheid, C.
- Biotechnology and Bioengineering, Vol. 95, Issue 3
The molecular nature of damage by oxygen to freeze-dried Escherichia coli
journal, February 1975
- Israeli, E.; Kohn, A.; Gitelman, Janina
- Cryobiology, Vol. 12, Issue 1
Membrane phase behavior of Escherichia coli during desiccation, rehydration, and growth recovery
journal, November 2009
- Scherber, Cally M.; Schottel, Janet L.; Aksan, Alptekin
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1788, Issue 11
Immobilized viable microbial cells: from the process to the proteome… or the cart before the horse
journal, November 2004
- Junter, Guy-Alain; Jouenne, Thierry
- Biotechnology Advances, Vol. 22, Issue 8
Preservation of micro-organisms by drying; A review
journal, August 2006
- Morgan, C. A.; Herman, N.; White, P. A.
- Journal of Microbiological Methods, Vol. 66, Issue 2
Paper Strip Whole Cell Biosensors: A Portable Test for the Semiquantitative Detection of Bacterial Quorum Signaling Molecules
journal, June 2010
- Struss, Anjali; Pasini, Patrizia; Ensor, C. Mark
- Analytical Chemistry, Vol. 82, Issue 11
Evidence of membrane lipid oxidation of spray-dried Lactobacillus bulgaricus during storage
journal, January 1996
- Teixeira, P.; Castro, H.; Kirby, R.
- Letters in Applied Microbiology, Vol. 22, Issue 1
Trehalose and sucrose protect both membranes and proteins in intact bacteria during drying.
journal, January 1995
- Leslie, S. B.; Israeli, E.; Lighthart, B.
- Applied and environmental microbiology, Vol. 61, Issue 10
Bioactive Ceramics - New Processing Technologies for Immobilization of Microorganisms for Filtration and Bioreactor Applications
journal, April 2007
- Koch, Dietmar; Soltmann, Christian; Grathwohl, Georg
- Key Engineering Materials, Vol. 336-338
Works referencing / citing this record:
The effects of Rhodopseudomonas palustris PSB06 and CGA009 with different agricultural applications on rice growth and rhizosphere bacterial communities
journal, October 2019
- Luo, Luyun; Wang, Pei; Zhai, Zhongying
- AMB Express, Vol. 9, Issue 1
The effects of Rhodopseudomonas palustris PSB06 and CGA009 with different agricultural applications on rice growth and rhizosphere bacterial communities
journal, October 2019
- Luo, Luyun; Wang, Pei; Zhai, Zhongying
- AMB Express, Vol. 9, Issue 1