Effect of high pressure homogenization on aqueous phase solvent extraction of lipids from Nannochloris Oculata microalgae

Samarasinghe, Nalin; Fernando, Sandun; Faulkner, William B.

doi:10.11648/j.jenr.20120101.11

Title: Effect of high pressure homogenization on aqueous phase solvent extraction of lipids from Nannochloris Oculata microalgae

Abstract

The ability to extract lipids from high-moisture Nannochloris Oculata algal biomass disrupted with high pressure homogenization was investigated. During the first phase, the effect of high pressure homogenization (system pressure and number of passes) on disrupting aqueous algae (of different concentrations and degree of stress) was investigated. Secondly, the effect of degree of cell wall disruption on the amount of lipids extracted with three solvents, namely: hexane, dichloromethane and chloroform, were compared. Studies reveled that high pressure homogenization is effective on cell disruption while the amount of system pressure being the most significant factor affecting the degree of cell breakage. Although the number of passes had some impact, the level of disruption seemed to level-off after a certain number of passes. The study revealed that slightly polar solvents (such as chloroform and dichloromethane) performed better in aqueous-phase lipid extractions as compared to hexane. Also, it was revealed that it was not necessary to disrupt the algal cells completely to achieve appreciable levels of lipid yields. In fact, conditions that exerted only 20% of the cells to completely disrupt, allowed sufficient damage to liberate most of the lipids contained in the remainder of the cells.

Authors:

Samarasinghe, Nalin ^[1]; Fernando, Sandun ^[1]; Faulkner, William B. ^[1]

Texas A & M Univ., College Station, TX (United States). Dept. of Biological and Agricultural Engineering

Publication Date:: Sat Dec 01 00:00:00 EST 2012

Research Org.:: Texas A&M AgriLife Research, College Station, TX (United States)

Sponsoring Org.:: USDOE Office of Energy Efficiency and Renewable Energy (EERE)

OSTI Identifier:: 1093832

Report Number(s):: DOE-DANF-0003046-P4
Journal ID: ISSN 2330-7366; 28302-P

Grant/Contract Number:: EE0003046

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Energy and Natural Resources

Additional Journal Information:: Journal Volume: 1; Journal Issue: 1; Journal ID: ISSN 2330-7366

Publisher:: SciencePG

Country of Publication:: United States

Language:: English

Subject:: 09 BIOMASS FUELS; microalgae; homogenization; extraction; lipids

Citation Formats


                    Samarasinghe, Nalin, Fernando, Sandun, and Faulkner, William B. Effect of high pressure homogenization on aqueous phase solvent extraction of lipids from Nannochloris Oculata microalgae.  United States: N. p., 2012. 
Web.  doi:10.11648/j.jenr.20120101.11.

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                    Samarasinghe, Nalin, Fernando, Sandun, & Faulkner, William B. Effect of high pressure homogenization on aqueous phase solvent extraction of lipids from Nannochloris Oculata microalgae.  United States.  https://doi.org/10.11648/j.jenr.20120101.11

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                    Samarasinghe, Nalin, Fernando, Sandun, and Faulkner, William B. Sat .  
"Effect of high pressure homogenization on aqueous phase solvent extraction of lipids from Nannochloris Oculata microalgae".  United States.  https://doi.org/10.11648/j.jenr.20120101.11.  https://www.osti.gov/servlets/purl/1093832.

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@article{osti_1093832,

  title        = {Effect of high pressure homogenization on aqueous phase solvent extraction of lipids from Nannochloris Oculata microalgae},

  author       = {Samarasinghe, Nalin and Fernando, Sandun and Faulkner, William B.},

  abstractNote = {The ability to extract lipids from high-moisture Nannochloris Oculata algal biomass disrupted with high pressure homogenization was investigated. During the first phase, the effect of high pressure homogenization (system pressure and number of passes) on disrupting aqueous algae (of different concentrations and degree of stress) was investigated. Secondly, the effect of degree of cell wall disruption on the amount of lipids extracted with three solvents, namely: hexane, dichloromethane and chloroform, were compared. Studies reveled that high pressure homogenization is effective on cell disruption while the amount of system pressure being the most significant factor affecting the degree of cell breakage. Although the number of passes had some impact, the level of disruption seemed to level-off after a certain number of passes. The study revealed that slightly polar solvents (such as chloroform and dichloromethane) performed better in aqueous-phase lipid extractions as compared to hexane. Also, it was revealed that it was not necessary to disrupt the algal cells completely to achieve appreciable levels of lipid yields. In fact, conditions that exerted only 20% of the cells to completely disrupt, allowed sufficient damage to liberate most of the lipids contained in the remainder of the cells.},

  doi          = {10.11648/j.jenr.20120101.11},

  journal      = {Journal of Energy and Natural Resources},

  number       = 1,

  volume       = 1,

  place        = {United States},

  year         = {Sat Dec 01 00:00:00 EST 2012},

  month        = {Sat Dec 01 00:00:00 EST 2012}

}

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Works referencing / citing this record:

Renewable Sources of Plant Biostimulation: Microalgae as a Sustainable Means to Improve Crop Performance
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Innovative Alternative Technologies to Extract Carotenoids from Microalgae and Seaweeds
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Marine Drugs, Vol. 14, Issue 11
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Renewable Sources of Plant Biostimulation: Microalgae as a Sustainable Means to Improve Crop Performance
journal, December 2018

Chiaiese, Pasquale; Corrado, Giandomenico; Colla, Giuseppe
Frontiers in Plant Science, Vol. 9
DOI: 10.3389/fpls.2018.01782

Innovative Alternative Technologies to Extract Carotenoids from Microalgae and Seaweeds
journal, November 2016

Poojary, Mahesha; Barba, Francisco; Aliakbarian, Bahar
Marine Drugs, Vol. 14, Issue 11
DOI: 10.3390/md14110214

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Title: Effect of high pressure homogenization on aqueous phase solvent extraction of lipids from Nannochloris Oculata microalgae

Abstract

Citation Formats

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