Kinetically Controlled Nanostructure Formation in Self-Assembled Globular Protein–Polymer Diblock Copolymers

Thomas, Carla S.; Xu, Liza; Olsen, Bradley D.

doi:10.1021/bm300763x

Title: Kinetically Controlled Nanostructure Formation in Self-Assembled Globular Protein–Polymer Diblock Copolymers

Abstract

Aqueous processing of globular protein–polymer diblock copolymers into solid-state materials and subsequent solvent annealing enables kinetic and thermodynamic control of nanostructure formation to produce block copolymer morphologies that maintain a high degree of protein fold and function. When model diblock copolymers composed of mCherry-b-poly(N-isopropylacrylamide) are used, orthogonal control over solubility of the protein block through changes in pH and the polymer block through changes in temperature is demonstrated during casting and solvent annealing. Hexagonal cylinders, perforated lamellae, lamellae, or hexagonal and disordered micellar phases are observed, depending on the coil fraction of the block copolymer and the kinetic pathway used for self-assembly. Here, good solvents for the polymer block produce ordered structures reminiscent of coil–coil diblock copolymers, while an unfavorable solvent results in kinetically trapped micellar structures. Decreasing solvent quality for the protein improves long-range ordering, suggesting that the strength of protein interactions influences nanostructure formation. Subsequent solvent annealing results in evolution of the nanostructures, with the best ordering and the highest protein function observed when annealing in a good solvent for both blocks. While protein secondary structure was found to be almost entirely preserved for all processing pathways, UV–vis spectroscopy of solid-state films indicates that using a good solventmore »« less

Authors:

Thomas, Carla S. ^[1]; Xu, Liza ^[1]; Olsen, Bradley D. ^[1]

Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Publication Date:: Tue Aug 28 00:00:00 EDT 2012

Research Org.:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Org.:: USDOE Office of Science (SC)

OSTI Identifier:: 1069699

Report Number(s):: BNL-100271-2013-JA
Journal ID: ISSN 1525-7797

Grant/Contract Number:: AC02-98CH10886

Resource Type:: Accepted Manuscript

Journal Name:: Biomacromolecules

Additional Journal Information:: Journal Volume: 13; Journal Issue: 9; Journal ID: ISSN 1525-7797

Publisher:: American Chemical Society

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; block copolymer; self-assembly; protein-polymer conjugate; mCherry; PNIPAM

Citation Formats


                    Thomas, Carla S., Xu, Liza, and Olsen, Bradley D. Kinetically Controlled Nanostructure Formation in Self-Assembled Globular Protein–Polymer Diblock Copolymers.  United States: N. p., 2012. 
Web.  doi:10.1021/bm300763x.

Copy to clipboard


                    Thomas, Carla S., Xu, Liza, & Olsen, Bradley D. Kinetically Controlled Nanostructure Formation in Self-Assembled Globular Protein–Polymer Diblock Copolymers.  United States.  https://doi.org/10.1021/bm300763x

Copy to clipboard


                    Thomas, Carla S., Xu, Liza, and Olsen, Bradley D. Tue .  
"Kinetically Controlled Nanostructure Formation in Self-Assembled Globular Protein–Polymer Diblock Copolymers".  United States.  https://doi.org/10.1021/bm300763x.  https://www.osti.gov/servlets/purl/1069699.

Copy to clipboard


                    
@article{osti_1069699,

  title        = {Kinetically Controlled Nanostructure Formation in Self-Assembled Globular Protein–Polymer Diblock Copolymers},

  author       = {Thomas, Carla S. and Xu, Liza and Olsen, Bradley D.},

  abstractNote = {Aqueous processing of globular protein–polymer diblock copolymers into solid-state materials and subsequent solvent annealing enables kinetic and thermodynamic control of nanostructure formation to produce block copolymer morphologies that maintain a high degree of protein fold and function. When model diblock copolymers composed of mCherry-b-poly(N-isopropylacrylamide) are used, orthogonal control over solubility of the protein block through changes in pH and the polymer block through changes in temperature is demonstrated during casting and solvent annealing. Hexagonal cylinders, perforated lamellae, lamellae, or hexagonal and disordered micellar phases are observed, depending on the coil fraction of the block copolymer and the kinetic pathway used for self-assembly. Here, good solvents for the polymer block produce ordered structures reminiscent of coil–coil diblock copolymers, while an unfavorable solvent results in kinetically trapped micellar structures. Decreasing solvent quality for the protein improves long-range ordering, suggesting that the strength of protein interactions influences nanostructure formation. Subsequent solvent annealing results in evolution of the nanostructures, with the best ordering and the highest protein function observed when annealing in a good solvent for both blocks. While protein secondary structure was found to be almost entirely preserved for all processing pathways, UV–vis spectroscopy of solid-state films indicates that using a good solvent for the protein block enables up to 70% of the protein to be retained in its functional form.},

  doi          = {10.1021/bm300763x},

  journal      = {Biomacromolecules},

  number       = 9,

  volume       = 13,

  place        = {United States},

  year         = {Tue Aug 28 00:00:00 EDT 2012},

  month        = {Tue Aug 28 00:00:00 EDT 2012}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (DOE)

Publisher's Version of Record

https://doi.org/10.1021/bm300763x

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 56 works

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Integration of Layered Redox Proteins and Conductive Supports for Bioelectronic Applications
journal, April 2000

Willner, Itamar; Katz, Eugenii
Angewandte Chemie International Edition, Vol. 39, Issue 7
DOI: 10.1002/(SICI)1521-3773(20000403)39:7<1180::AID-ANIE1180>3.0.CO;2-E

Synthesis of chiral pharmaceutical intermediates by biocatalysis
journal, March 2008

Patel, Ramesh N.
Coordination Chemistry Reviews, Vol. 252, Issue 5-7
DOI: 10.1016/j.ccr.2007.10.031

Reversible interconversion of carbon dioxide and formate by an electroactive enzyme
journal, July 2008

Reda, T.; Plugge, C. M.; Abram, N. J.
Proceedings of the National Academy of Sciences, Vol. 105, Issue 31, p. 10654-10658
DOI: 10.1073/pnas.0801290105

Integration of Photosynthetic Protein Molecular Complexes in Solid-State Electronic Devices
journal, June 2004

Das, Rupa; Kiley, Patrick J.; Segal, Michael
Nano Letters, Vol. 4, Issue 6
DOI: 10.1021/nl049579f

Recent advances in nanostructured biocatalysts
journal, April 2009

Ge, Jun; Lu, Diannan; Liu, Zhixia
Biochemical Engineering Journal, Vol. 44, Issue 1
DOI: 10.1016/j.bej.2009.01.002

Template Synthesis of Nanostructured Materials via Layer-by-Layer Assembly ^†
journal, February 2008

Wang, Yajun; Angelatos, Alexandra S.; Caruso, Frank
Chemistry of Materials, Vol. 20, Issue 3
DOI: 10.1021/cm7024813

Dynamic Interfacial Properties of Poly(ethylene glycol)-Modified Ferritin at the Solid/Liquid Interface
journal, July 2008

Kumashiro, Yoshikazu; Ikezoe, Yasuhiro; Tamada, Kaoru
The Journal of Physical Chemistry B, Vol. 112, Issue 28
DOI: 10.1021/jp7104506

Controlled Assembly for Well-Defined 3D Bioarchitecture Using Two Active Enzymes
journal, February 2010

Kim, Dong Chung; Sohn, Jung Inn; Zhou, Dejian
ACS Nano, Vol. 4, Issue 3
DOI: 10.1021/nn900610u

Electron-Transfer Dynamics of Photosynthetic Reaction Centers in Thermoresponsive Soft Materials
journal, December 2005

Laible, Philip D.; Kelley, Richard F.; Wasielewski, Michael R.
The Journal of Physical Chemistry B, Vol. 109, Issue 49
DOI: 10.1021/jp053884n

Characterization of Sol−Gel-Encapsulated Proteins Using Small-Angle Neutron Scattering
journal, September 2009

Luo, Guangming; Zhang, Qiu; Castillo, Alexis Rae Del
ACS Applied Materials & Interfaces, Vol. 1, Issue 10
DOI: 10.1021/am900430v

Immobilization of Human Carbonic Anhydrase on Gold Nanoparticles Assembled onto Amine/Thiol-Functionalized Mesoporous SBA-15 for Biomimetic Sequestration of CO ₂
journal, May 2011

Vinoba, Mari; Lim, Kyoung Soo; Lee, Si Hyun
Langmuir, Vol. 27, Issue 10
DOI: 10.1021/la105029h

Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of Enzymes
journal, July 2011

Rodrigues, Rafael C.; Berenguer-Murcia, Ángel; Fernandez-Lafuente, Roberto
Advanced Synthesis & Catalysis, Vol. 353, Issue 13
DOI: 10.1002/adsc.201100163

Self-Assembly and Supramolecular Assembly in Nanophase Separated Polymers and Thin Films
book, January 2008

Dutta, Naba K.; Choudhury, Namita Roy
Functional Nanostructures
DOI: 10.1007/978-0-387-48805-9_5

Interactions in protein solutions
journal, April 2006

Sear, R.
Current Opinion in Colloid & Interface Science, Vol. 11, Issue 1
DOI: 10.1016/j.cocis.2005.09.003

In Situ Preparation of Protein−“Smart” Polymer Conjugates with Retention of Bioactivity
journal, December 2005

Heredia, Karina L.; Bontempo, Debora; Ly, Tiffany
Journal of the American Chemical Society, Vol. 127, Issue 48
DOI: 10.1021/ja054482w

Biosynthetic-Synthetic Polymer Conjugates
journal, April 2007

van Hest, Jan C. M.
Polymer Reviews, Vol. 47, Issue 1
DOI: 10.1080/15583720601109578

Advances in Bioconjugation
journal, January 2010

Kalia, Jeet; Raines, Ronald
Current Organic Chemistry, Vol. 14, Issue 2
DOI: 10.2174/138527210790069839

Fabrication of a Thermoresponsive Biohybrid Double Hydrophilic Block Copolymer by a Cofactor Reconstitution Approach
journal, October 2010

Wan, Xuejuan; Liu, Shiyong
Macromolecular Rapid Communications, Vol. 31, Issue 23
DOI: 10.1002/marc.201000340

Anisotropic incorporation of lipid-anchored myoglobin into a phospholipid bilayer membrane
journal, June 1993

Hamachi, Itaru; Nakamura, Kentaro; Fujita, Akio
Journal of the American Chemical Society, Vol. 115, Issue 12
DOI: 10.1021/ja00065a004

An overview of protein–polymer particles
journal, January 2011

Boyer, Cyrille; Huang, Xin; Whittaker, Michael R.
Soft Matter, Vol. 7, Issue 5
DOI: 10.1039/C0SM00412J

Synthesis of Well-Defined (Nitrilotriacetic Acid)-End-Functionalized Polystyrenes and Their Bioconjugation with Histidine-Tagged Green Fluorescent Proteins
journal, June 2011

Cho, Hong Y.; Kadir, Mohammad Abdul; Kim, Bong-Soo
Macromolecules, Vol. 44, Issue 12
DOI: 10.1021/ma200480f

Stability of responsive polymer–protein bioconjugates
journal, April 2010

Shakya, Akhilesh Kumar; Sami, Haider; Srivastava, Akshay
Progress in Polymer Science, Vol. 35, Issue 4
DOI: 10.1016/j.progpolymsci.2010.01.003

Self-Assembled Architectures from Biohybrid Triblock Copolymers
journal, February 2007

Reynhout, Irene C.; Cornelissen, Jeroen J. L. M.; Nolte, Roeland J. M.
Journal of the American Chemical Society, Vol. 129, Issue 8
DOI: 10.1021/ja066790f

Synthesis of Polymer−Biohybrids: From Small to Giant Surfactants
journal, June 2009

Reynhout, Irene C.; Cornelissen, Jeroen J. L. M.; Nolte, Roeland J. M.
Accounts of Chemical Research, Vol. 42, Issue 6
DOI: 10.1021/ar800143a

Bionanoparticles and hybrid materials: tailored structural properties, self-assembly, materials and developments in the field
journal, January 2011

van Rijn, Patrick; Böker, Alexander
Journal of Materials Chemistry, Vol. 21, Issue 42
DOI: 10.1039/c1jm11433f

Hybrid Capsules via Self-Assembly of Thermoresponsive and Interfacially Active Bionanoparticle-Polymer Conjugates
journal, March 2011

Mougin, Nathalie C.; van Rijn, Patrick; Park, Hyunji
Advanced Functional Materials, Vol. 21, Issue 13
DOI: 10.1002/adfm.201002315

Conjugation of RAFT-generated polymers to proteins by two consecutive thiol–ene reactions
journal, January 2010

Li, Ming; De, Priyadarsi; Li, Hongmei
Polymer Chemistry, Vol. 1, Issue 6
DOI: 10.1039/c0py00025f

Reversible Meso-Scale Smart Polymer−Protein Particles of Controlled Sizes
journal, July 2004

Kulkarni, Samarth; Schilli, Christine; Müller, Axel H. E.
Bioconjugate Chemistry, Vol. 15, Issue 4
DOI: 10.1021/bc034215k

Orientation of myoglobin in cast multibilayer membranes of amphiphilic molecules
journal, July 1995

Nassar, Alaa-Eldin F.; Zhang, Zhe; Chynwat, Veeradej
The Journal of Physical Chemistry, Vol. 99, Issue 27
DOI: 10.1021/j100027a049

Directed co-assembly of hemeproteins with amphiphilic block copolymers toward functional biomolecular materials
journal, January 2011

Presley, Andrew D.; Chang, Joseph J.; Xu, Ting
Soft Matter, Vol. 7, Issue 1
DOI: 10.1039/C0SM00817F

Ferritin-Polymer Conjugates: Grafting Chemistry and Integration into Nanoscale Assemblies
journal, August 2010

Hu, Yunxia; Samanta, Debasis; Parelkar, Sangram S.
Advanced Functional Materials, Vol. 20, Issue 20
DOI: 10.1002/adfm.201000958

Nanopatterned Protein Films Directed by Ionic Complexation with Water-Soluble Diblock Copolymers
journal, May 2012

Kim, Bokyung; Lam, Christopher N.; Olsen, Bradley D.
Macromolecules, Vol. 45, Issue 11
DOI: 10.1021/ma2024914

Solid-State Nanostructured Materials from Self-Assembly of a Globular Protein–Polymer Diblock Copolymer
journal, June 2011

Thomas, Carla S.; Glassman, Matthew J.; Olsen, Bradley D.
ACS Nano, Vol. 5, Issue 7
DOI: 10.1021/nn2013673

Highly Oriented and Ordered Arrays from Block Copolymers via Solvent Evaporation
journal, February 2004

Kim, S. H.; Misner, M. J.; Xu, T.
Advanced Materials, Vol. 16, Issue 3, p. 226-231
DOI: 10.1002/adma.200304906

Solvent annealing thin films of poly(isoprene-b-lactide)
journal, November 2005

Cavicchi, Kevin A.; Berthiaume, Keith J.; Russell, Thomas P.
Polymer, Vol. 46, Issue 25
DOI: 10.1016/j.polymer.2005.09.072

Patterning with block copolymer thin films
journal, February 2005

Segalman, Rachel A.
Materials Science and Engineering: R: Reports, Vol. 48, Issue 6, p. 191-226
DOI: 10.1016/j.mser.2004.12.003

Self-assembly of block copolymer thin films
journal, June 2010

Albert, Julie N. L.; Epps, Thomas H.
Materials Today, Vol. 13, Issue 6, p. 24-33
DOI: 10.1016/S1369-7021(10)70106-1

Morphological Development in Solvent-Cast Polystyrene−Polybutadiene−Polystyrene (SBS) Triblock Copolymer Thin Films
journal, April 1998

Kim, G.; Libera, M.
Macromolecules, Vol. 31, Issue 8
DOI: 10.1021/ma971349i

The Full Phase Behavior for Block Copolymers in Solvents of Varying Selectivity
journal, June 2002

Lodge, Timothy P.; Pudil, Bryant; Hanley, Kenneth J.
Macromolecules, Vol. 35, Issue 12
DOI: 10.1021/ma0200975

Nature and consequences of protein–protein interactions in high protein concentration solutions
journal, June 2008

Saluja, Atul; Kalonia, Devendra S.
International Journal of Pharmaceutics, Vol. 358, Issue 1-2
DOI: 10.1016/j.ijpharm.2008.03.041

Examination of the secondary structure of proteins by deconvolved FTIR spectra
journal, March 1986

Byler, D. Michael; Susi, Heino
Biopolymers, Vol. 25, Issue 3
DOI: 10.1002/bip.360250307

The Use and Misuse of FTIR Spectroscopy in the Determination of Protein Structure
journal, January 1995

Jackson, Michael; Mantsch, Henry H.
Critical Reviews in Biochemistry and Molecular Biology, Vol. 30, Issue 2
DOI: 10.3109/10409239509085140

Approximations Leading to a Unified Exponential/Power-Law Approach to Small-Angle Scattering
journal, December 1995

Beaucage, G.
Journal of Applied Crystallography, Vol. 28, Issue 6
DOI: 10.1107/S0021889895005292

Hard-sphere interactions between spherical domains in diblock copolymers
journal, September 1984

Kinning, David J.; Thomas, Edwin L.
Macromolecules, Vol. 17, Issue 9
DOI: 10.1021/ma00139a013

Self-Assembly and Stress Relaxation in Acrylic Triblock Copolymer Gels
journal, January 2007

Seitz, Michelle E.; Burghardt, Wesley R.; Faber, K. T.
Macromolecules, Vol. 40, Issue 4
DOI: 10.1021/ma061993+

Novel Chromophores and Buried Charges Control Color in mFruits ^† ^, ^‡
journal, August 2006

Shu, Xiaokun; Shaner, Nathan C.; Yarbrough, Corinne A.
Biochemistry, Vol. 45, Issue 32
DOI: 10.1021/bi060773l

Single Polymer Chain Elongation of Poly( N -isopropylacrylamide) and Poly(acrylamide) by Atomic Force Microscopy
journal, November 2000

Zhang, Wenke; Zou, Shan; Wang, Chi
The Journal of Physical Chemistry B, Vol. 104, Issue 44
DOI: 10.1021/jp000459f

The Conformation of the Poly(ethylene glycol) Chain in Mono-PEGylated Lysozyme and Mono-PEGylated Human Growth Hormone
journal, November 2011

Pai, Sheetal S.; Hammouda, Boualem; Hong, Kunlun
Bioconjugate Chemistry, Vol. 22, Issue 11
DOI: 10.1021/bc2003583

Phase Behavior of a Block Copolymer in Solvents of Varying Selectivity
journal, August 2000

Hanley, Kenneth J.; Lodge, Timothy P.; Huang, Ching-I
Macromolecules, Vol. 33, Issue 16
DOI: 10.1021/ma000318b

Ordering Dynamics of a Symmetric Polystyrene- block -polyisoprene. 2. Real-Space Analysis on the Formation of Lamellar Microdomain
journal, June 1998

Sakamoto, Naoki; Hashimoto, Takeji
Macromolecules, Vol. 31, Issue 12
DOI: 10.1021/ma980037s

Block Copolymers—Designer Soft Materials
journal, February 1999

Bates, Frank S.; Fredrickson, Glenn H.
Physics Today, Vol. 52, Issue 2
DOI: 10.1063/1.882522

Enabling nanotechnology with self assembled block copolymer patterns
journal, October 2003

Park, Cheolmin; Yoon, Jongseung; Thomas, Edwin L.
Polymer, Vol. 44, Issue 22, p. 6725-6760
DOI: 10.1016/j.polymer.2003.08.011

Structure and Reactivity of the Chromophore of a GFP-like Chromoprotein from Condylactis gigantea ^†
journal, June 2006

Pakhomov, Alexey A.; Pletneva, Nadezhda V.; Balashova, Tamara A.
Biochemistry, Vol. 45, Issue 23
DOI: 10.1021/bi060207q

The structure of the chromophore within DsRed, a red fluorescent protein from coral
journal, October 2000

Gross, L. A.; Baird, G. S.; Hoffman, R. C.
Proceedings of the National Academy of Sciences, Vol. 97, Issue 22
DOI: 10.1073/pnas.97.22.11990

Photoactivation mechanism of PAmCherry based on crystal structures of the protein in the dark and fluorescent states
journal, November 2009

Subach, Fedor V.; Malashkevich, Vladimir N.; Zencheck, Wendy D.
Proceedings of the National Academy of Sciences, Vol. 106, Issue 50
DOI: 10.1073/pnas.0909204106

Insight into the Common Mechanism of the Chromophore Formation in the Red Fluorescent Proteins: The Elusive Blue Intermediate Revealed
journal, January 2012

Bravaya, Ksenia B.; Subach, Oksana M.; Korovina, Nadezhda
Journal of the American Chemical Society, Vol. 134, Issue 5
DOI: 10.1021/ja2114568

Protein–excipient interactions: Mechanisms and biophysical characterization applied to protein formulation development
journal, October 2011

Kamerzell, Tim J.; Esfandiary, Reza; Joshi, Sangeeta B.
Advanced Drug Delivery Reviews, Vol. 63, Issue 13
DOI: 10.1016/j.addr.2011.07.006

Fourier Transform Infrared Spectroscopic Analysis of Protein Secondary Structures
journal, August 2007

Kong, Jilie; Yu, Shaoning
Acta Biochimica et Biophysica Sinica, Vol. 39, Issue 8
DOI: 10.1111/j.1745-7270.2007.00320.x

FTIR characterization of the secondary structure of proteins encapsulated within PLGA microspheres1An article of related interest has been published by Yang et al. in J. Pharm. Sci., 88(2), Feb. 1999, accepted Nov. 1998.1
journal, April 1999

Fu, Karen; Griebenow, Kai; Hsieh, Lisa
Journal of Controlled Release, Vol. 58, Issue 3
DOI: 10.1016/S0168-3659(98)00192-8

Characterization of the Pressure-induced Intermediate and Unfolded State of Red-shifted Green Fluorescent Protein—A Static and Kinetic FTIR, UV/VIS and Fluorescence Spectroscopy Study
journal, July 2003

Herberhold, H.; Marchal, S.; Lange, R.
Journal of Molecular Biology, Vol. 330, Issue 5
DOI: 10.1016/S0022-2836(03)00657-0

What vibrations tell about proteins
journal, November 2002

Barth, Andreas; Zscherp, Christian
Quarterly Reviews of Biophysics, Vol. 35, Issue 4
DOI: 10.1017/S0033583502003815

Heat Capacity of Spider Silk-Like Block Copolymers
journal, July 2011

Huang, Wenwen; Krishnaji, Sreevidhya; Hu, Xiao
Macromolecules, Vol. 44, Issue 13
DOI: 10.1021/ma200563t

FTIR and nDSC as Analytical Tools for High-Concentration Protein Formulations
journal, May 2006

Matheus, Susanne; Friess, Wolfgang; Mahler, Hanns-Christian
Pharmaceutical Research, Vol. 23, Issue 6
DOI: 10.1007/s11095-006-0142-8

Works referencing / citing this record:

Responsive polymers for biosensing and protein delivery
journal, January 2014

Islam, Molla R.; Gao, Yongfeng; Li, Xue
J. Mater. Chem. B, Vol. 2, Issue 17
DOI: 10.1039/c3tb21657h

Elastin-like polypeptides as building motifs toward designing functional nanobiomaterials
journal, January 2019

Le, Duc H. T.; Sugawara-Narutaki, Ayae
Molecular Systems Design & Engineering, Vol. 4, Issue 3
DOI: 10.1039/c9me00002j

Photochemistry for Well-Defined Polymers in Aqueous Media: From Fundamentals to Polymer Nanoparticles to Bioconjugates
journal, May 2018

Burridge, Kevin M.; Wright, Thaiesha A.; Page, Richard C.
Macromolecular Rapid Communications, Vol. 39, Issue 12
DOI: 10.1002/marc.201800093

Topological Effects on Globular Protein-ELP Fusion Block Copolymer Self-Assembly
journal, December 2014

Qin, Guokui; Glassman, Matthew J.; Lam, Christopher N.
Advanced Functional Materials, Vol. 25, Issue 5
DOI: 10.1002/adfm.201403453

Self-assembled nanostructures from amphiphilic globular protein–polymer hybrids
journal, September 2017

Cao, Qi; He, Naipu; Wang, Yue
Polymer Bulletin, Vol. 75, Issue 6
DOI: 10.1007/s00289-017-2176-y

Phase transitions in concentrated solution self-assembly of globular protein–polymer block copolymers
journal, January 2013

Lam, Christopher N.; Olsen, Bradley D.
Soft Matter, Vol. 9, Issue 8
DOI: 10.1039/c2sm27459k

Enzyme–Polymer Conjugates as Robust Pickering Interfacial Biocatalysts for Efficient Biotransformations and One‐Pot Cascade Reactions
journal, October 2018

Sun, Zhiyong; Glebe, Ulrich; Charan, Himanshu
Angewandte Chemie International Edition, Vol. 57, Issue 42
DOI: 10.1002/anie.201806049

Block copolymers for protein ordering
journal, January 2014

Malmström, Jenny; Travas-Sejdic, Jadranka
Journal of Applied Polymer Science, Vol. 131, Issue 14
DOI: 10.1002/app.40360

Enzyme–Polymer Conjugates as Robust Pickering Interfacial Biocatalysts for Efficient Biotransformations and One‐Pot Cascade Reactions
journal, September 2018

Sun, Zhiyong; Glebe, Ulrich; Charan, Himanshu
Angewandte Chemie, Vol. 130, Issue 42
DOI: 10.1002/ange.201806049

Self-Assembly of Globular-Protein-Containing Block Copolymers
journal, July 2013

Olsen, Bradley D.
Macromolecular Chemistry and Physics, Vol. 214, Issue 15
DOI: 10.1002/macp.201300235

Micellar‐Mediated Block Copolymer Ordering Dynamics Revealed by In Situ Grazing Incidence Small‐Angle X‐Ray Scattering during Spin Coating
journal, March 2019

Fleury, Guillaume; Hermida‐Merino, Daniel; Jingjin, Dong
Advanced Functional Materials, Vol. 29, Issue 10
DOI: 10.1002/adfm.201806741

Similar Records in DOE PAGES and OSTI.GOV collections:

Coil fraction-dependent phase behaviour of a model globular protein–polymer diblock copolymer

Journal Article Thomas, Carla S. ; Olsen, Bradley D. - Soft Matter

The self-assembly of the model globular protein–polymer block copolymer mCherry-b-poly(N-isopropyl acrylamide) is explored across a range of polymer coil fractions from 0.21 to 0.82 to produce a phase diagram for these materials as a function of molecular composition. Overall, four types of morphologies were observed: hexagonally packed cylinders, perforated lamellae, lamellae, and disordered nanostructures. Across all coil fractions and morphologies, a lyotropic re-entrant order–disorder transition in water was observed, with disordered structures below 30 wt% and above 70 wt% and well-ordered morphologies at intermediate concentrations. Solid state samples prepared by solvent evaporation show moderately ordered structures similar to those observedmore »« less
https://doi.org/10.1039/C3SM52531G
Effect of Small Molecule Osmolytes on the Self-Assembly and Functionality of Globular Protein-Polymer Diblock Copolymers

Journal Article Thomas, Carla S. ; Xu, Liza ; Olsen, Bradley D. - Biomacromolecules

Blending the small molecule osmolytes glycerol and trehalose with the model globular protein–polymer block copolymer mCherry-b-poly(N-isopropyl acrylamide) (mCherry-b-PNIPAM) is demonstrated to improve protein functionality in self-assembled nanostructures. The incorporation of either additive into block copolymers results in functionality retention in the solid state of 80 and 100% for PNIPAM volume fractions of 40 and 55%, respectively. This represents a large improvement over the 50–60% functionality observed in the absence of any additive. Furthermore, glycerol decreases the thermal stability of block copolymer films by 15–20 °C, while trehalose results in an improvement in the thermal stability by 15–20 °C. These resultsmore »« less
https://doi.org/10.1021/bm400664t
Topology effects on protein–polymer block copolymer self-assembly

Journal Article Suguri, Takuya ; Olsen, Bradley D. - Polymer Chemistry

Bioconjugates made of the model red fluorescent protein mCherry and synthetic polymer blocks show that topology, i.e. the BA, BA₂, ABA and ABC chain structure of the block copolymers, where B represents the protein and A and C represent polymers, has a significant effect on ordering transitions and the type and size of nanostructures formed during microphase separation. ABA and ABC type block copolymers were synthesized by using two site-specific bioconjugation reactions: the thiol–ene reaction with a cysteine on mCherry and maleimide functionalized polymers, and the sortase A ligation reaction with an LPETG sequence at the C-terminus on mCherry andmore »« less
Cited by 9
https://doi.org/10.1039/C8PY01228H
Lyotropic Phase Behavior of Poly(ethylene oxide)-Poly(butadiene) Diblock Copolymers: Evolution of the Random Network Morphology

Journal Article Jain, Sumeet ; Dyrdahl, Mitchell H.E. ; Gong, Xiaobo ; ... - Macromolecules

The phase behavior of poly(ethylene oxide)-poly(butadiene) (PEO-PB) diblock copolymers mixed with water was studied using small-angle X-ray scattering (SAXS), cryogenic scanning electron microscopy (cryo-SEM), cryogenic transmission electron microscopy (cryo-TEM), and dynamic mechanical spectroscopy. Two sets of diblocks were synthesized by adding different lengths of PEO to hydroxy terminated PB with degrees of polymerization N{sub PB} = 46 and 170. Two-component mixtures were investigated as a function of block composition and copolymer molecular weight, between 1 and 100 wt % polymer content. Melt phase behavior is consistent with established theory and known experimental behavior for diblock copolymers. Various lyotropic liquid crystallinemore »« less
https://doi.org/10.1021/ma702322b
High-Performance Field-Effect Transistors Based on Polystyrene-b-Poly(3-hexylthiophene) Diblock Copolymers

Journal Article Geohegan, David B ; Sumpter, Bobby G ; Hong, Kunlun ; ... - ACS Nano

Polystyrene-b-poly(3-hexylthiophene) (PS-b-P3HT) block copolymers with fixed PS block length have been synthesized by combined atom transfer radical polymerization (ATRP) and Grignard metathesis (GRIM) polymerization. The self-assembled structures of these diblock copolymer thin films based on PS-b-P3HT have been studied by TEM, SAED, GIXD, AFM, and additionally by first principles modeling and simulation. These block copolymers undergo microphase separation and form nanostructured spheres, lamellae, nanofibers, or nanoribbons in the films dictated by the molecular weight of the P3HT block. Within the diblock copolymer thin film, PS blocks segregate to form amorphous domains, and the covalently bonded conjugated P3HT blocks exist asmore »« less

Similar Records

Title: Kinetically Controlled Nanostructure Formation in Self-Assembled Globular Protein–Polymer Diblock Copolymers

Abstract

Citation Formats

Integration of Layered Redox Proteins and Conductive Supports for Bioelectronic Applications journal, April 2000

Synthesis of chiral pharmaceutical intermediates by biocatalysis journal, March 2008

Reversible interconversion of carbon dioxide and formate by an electroactive enzyme journal, July 2008

Integration of Photosynthetic Protein Molecular Complexes in Solid-State Electronic Devices journal, June 2004

Recent advances in nanostructured biocatalysts journal, April 2009

Template Synthesis of Nanostructured Materials via Layer-by-Layer Assembly † journal, February 2008

Dynamic Interfacial Properties of Poly(ethylene glycol)-Modified Ferritin at the Solid/Liquid Interface journal, July 2008

Controlled Assembly for Well-Defined 3D Bioarchitecture Using Two Active Enzymes journal, February 2010

Electron-Transfer Dynamics of Photosynthetic Reaction Centers in Thermoresponsive Soft Materials journal, December 2005

Characterization of Sol−Gel-Encapsulated Proteins Using Small-Angle Neutron Scattering journal, September 2009

Immobilization of Human Carbonic Anhydrase on Gold Nanoparticles Assembled onto Amine/Thiol-Functionalized Mesoporous SBA-15 for Biomimetic Sequestration of CO 2 journal, May 2011

Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of Enzymes journal, July 2011

Self-Assembly and Supramolecular Assembly in Nanophase Separated Polymers and Thin Films book, January 2008

Interactions in protein solutions journal, April 2006

In Situ Preparation of Protein−“Smart” Polymer Conjugates with Retention of Bioactivity journal, December 2005

Biosynthetic-Synthetic Polymer Conjugates journal, April 2007

Advances in Bioconjugation journal, January 2010

Fabrication of a Thermoresponsive Biohybrid Double Hydrophilic Block Copolymer by a Cofactor Reconstitution Approach journal, October 2010

Anisotropic incorporation of lipid-anchored myoglobin into a phospholipid bilayer membrane journal, June 1993

An overview of protein–polymer particles journal, January 2011

Synthesis of Well-Defined (Nitrilotriacetic Acid)-End-Functionalized Polystyrenes and Their Bioconjugation with Histidine-Tagged Green Fluorescent Proteins journal, June 2011

Stability of responsive polymer–protein bioconjugates journal, April 2010

Self-Assembled Architectures from Biohybrid Triblock Copolymers journal, February 2007

Synthesis of Polymer−Biohybrids: From Small to Giant Surfactants journal, June 2009

Bionanoparticles and hybrid materials: tailored structural properties, self-assembly, materials and developments in the field journal, January 2011

Hybrid Capsules via Self-Assembly of Thermoresponsive and Interfacially Active Bionanoparticle-Polymer Conjugates journal, March 2011

Conjugation of RAFT-generated polymers to proteins by two consecutive thiol–ene reactions journal, January 2010

Reversible Meso-Scale Smart Polymer−Protein Particles of Controlled Sizes journal, July 2004

Orientation of myoglobin in cast multibilayer membranes of amphiphilic molecules journal, July 1995

Directed co-assembly of hemeproteins with amphiphilic block copolymers toward functional biomolecular materials journal, January 2011

Ferritin-Polymer Conjugates: Grafting Chemistry and Integration into Nanoscale Assemblies journal, August 2010

Nanopatterned Protein Films Directed by Ionic Complexation with Water-Soluble Diblock Copolymers journal, May 2012

Solid-State Nanostructured Materials from Self-Assembly of a Globular Protein–Polymer Diblock Copolymer journal, June 2011

Highly Oriented and Ordered Arrays from Block Copolymers via Solvent Evaporation journal, February 2004

Solvent annealing thin films of poly(isoprene-b-lactide) journal, November 2005

Patterning with block copolymer thin films journal, February 2005

Self-assembly of block copolymer thin films journal, June 2010

Morphological Development in Solvent-Cast Polystyrene−Polybutadiene−Polystyrene (SBS) Triblock Copolymer Thin Films journal, April 1998

The Full Phase Behavior for Block Copolymers in Solvents of Varying Selectivity journal, June 2002

Nature and consequences of protein–protein interactions in high protein concentration solutions journal, June 2008

Examination of the secondary structure of proteins by deconvolved FTIR spectra journal, March 1986

The Use and Misuse of FTIR Spectroscopy in the Determination of Protein Structure journal, January 1995

Approximations Leading to a Unified Exponential/Power-Law Approach to Small-Angle Scattering journal, December 1995

Hard-sphere interactions between spherical domains in diblock copolymers journal, September 1984

Self-Assembly and Stress Relaxation in Acrylic Triblock Copolymer Gels journal, January 2007

Novel Chromophores and Buried Charges Control Color in mFruits † , ‡ journal, August 2006

Single Polymer Chain Elongation of Poly( N -isopropylacrylamide) and Poly(acrylamide) by Atomic Force Microscopy journal, November 2000

The Conformation of the Poly(ethylene glycol) Chain in Mono-PEGylated Lysozyme and Mono-PEGylated Human Growth Hormone journal, November 2011

Phase Behavior of a Block Copolymer in Solvents of Varying Selectivity journal, August 2000

Ordering Dynamics of a Symmetric Polystyrene- block -polyisoprene. 2. Real-Space Analysis on the Formation of Lamellar Microdomain journal, June 1998

Block Copolymers—Designer Soft Materials journal, February 1999

Enabling nanotechnology with self assembled block copolymer patterns journal, October 2003

Structure and Reactivity of the Chromophore of a GFP-like Chromoprotein from Condylactis gigantea † journal, June 2006

The structure of the chromophore within DsRed, a red fluorescent protein from coral journal, October 2000

Photoactivation mechanism of PAmCherry based on crystal structures of the protein in the dark and fluorescent states journal, November 2009

Insight into the Common Mechanism of the Chromophore Formation in the Red Fluorescent Proteins: The Elusive Blue Intermediate Revealed journal, January 2012

Protein–excipient interactions: Mechanisms and biophysical characterization applied to protein formulation development journal, October 2011

Fourier Transform Infrared Spectroscopic Analysis of Protein Secondary Structures journal, August 2007

FTIR characterization of the secondary structure of proteins encapsulated within PLGA microspheres1An article of related interest has been published by Yang et al. in J. Pharm. Sci., 88(2), Feb. 1999, accepted Nov. 1998.1 journal, April 1999

Characterization of the Pressure-induced Intermediate and Unfolded State of Red-shifted Green Fluorescent Protein—A Static and Kinetic FTIR, UV/VIS and Fluorescence Spectroscopy Study journal, July 2003

What vibrations tell about proteins journal, November 2002

Heat Capacity of Spider Silk-Like Block Copolymers journal, July 2011

FTIR and nDSC as Analytical Tools for High-Concentration Protein Formulations journal, May 2006

Responsive polymers for biosensing and protein delivery journal, January 2014

Elastin-like polypeptides as building motifs toward designing functional nanobiomaterials journal, January 2019

Photochemistry for Well-Defined Polymers in Aqueous Media: From Fundamentals to Polymer Nanoparticles to Bioconjugates journal, May 2018

Topological Effects on Globular Protein-ELP Fusion Block Copolymer Self-Assembly journal, December 2014

Self-assembled nanostructures from amphiphilic globular protein–polymer hybrids journal, September 2017

Phase transitions in concentrated solution self-assembly of globular protein–polymer block copolymers journal, January 2013

Enzyme–Polymer Conjugates as Robust Pickering Interfacial Biocatalysts for Efficient Biotransformations and One‐Pot Cascade Reactions journal, October 2018

Block copolymers for protein ordering journal, January 2014

Enzyme–Polymer Conjugates as Robust Pickering Interfacial Biocatalysts for Efficient Biotransformations and One‐Pot Cascade Reactions journal, September 2018

Self-Assembly of Globular-Protein-Containing Block Copolymers journal, July 2013

Micellar‐Mediated Block Copolymer Ordering Dynamics Revealed by In Situ Grazing Incidence Small‐Angle X‐Ray Scattering during Spin Coating journal, March 2019

Integration of Layered Redox Proteins and Conductive Supports for Bioelectronic Applications
journal, April 2000

Synthesis of chiral pharmaceutical intermediates by biocatalysis
journal, March 2008

Reversible interconversion of carbon dioxide and formate by an electroactive enzyme
journal, July 2008

Integration of Photosynthetic Protein Molecular Complexes in Solid-State Electronic Devices
journal, June 2004

Recent advances in nanostructured biocatalysts
journal, April 2009

Template Synthesis of Nanostructured Materials via Layer-by-Layer Assembly ^†
journal, February 2008

Dynamic Interfacial Properties of Poly(ethylene glycol)-Modified Ferritin at the Solid/Liquid Interface
journal, July 2008

Controlled Assembly for Well-Defined 3D Bioarchitecture Using Two Active Enzymes
journal, February 2010

Electron-Transfer Dynamics of Photosynthetic Reaction Centers in Thermoresponsive Soft Materials
journal, December 2005

Characterization of Sol−Gel-Encapsulated Proteins Using Small-Angle Neutron Scattering
journal, September 2009

Immobilization of Human Carbonic Anhydrase on Gold Nanoparticles Assembled onto Amine/Thiol-Functionalized Mesoporous SBA-15 for Biomimetic Sequestration of CO ₂
journal, May 2011

Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of Enzymes
journal, July 2011

Self-Assembly and Supramolecular Assembly in Nanophase Separated Polymers and Thin Films
book, January 2008

Interactions in protein solutions
journal, April 2006

In Situ Preparation of Protein−“Smart” Polymer Conjugates with Retention of Bioactivity
journal, December 2005

Biosynthetic-Synthetic Polymer Conjugates
journal, April 2007

Advances in Bioconjugation
journal, January 2010

Fabrication of a Thermoresponsive Biohybrid Double Hydrophilic Block Copolymer by a Cofactor Reconstitution Approach
journal, October 2010

Anisotropic incorporation of lipid-anchored myoglobin into a phospholipid bilayer membrane
journal, June 1993

An overview of protein–polymer particles
journal, January 2011

Synthesis of Well-Defined (Nitrilotriacetic Acid)-End-Functionalized Polystyrenes and Their Bioconjugation with Histidine-Tagged Green Fluorescent Proteins
journal, June 2011

Stability of responsive polymer–protein bioconjugates
journal, April 2010

Self-Assembled Architectures from Biohybrid Triblock Copolymers
journal, February 2007

Synthesis of Polymer−Biohybrids: From Small to Giant Surfactants
journal, June 2009

Bionanoparticles and hybrid materials: tailored structural properties, self-assembly, materials and developments in the field
journal, January 2011

Hybrid Capsules via Self-Assembly of Thermoresponsive and Interfacially Active Bionanoparticle-Polymer Conjugates
journal, March 2011

Conjugation of RAFT-generated polymers to proteins by two consecutive thiol–ene reactions
journal, January 2010

Reversible Meso-Scale Smart Polymer−Protein Particles of Controlled Sizes
journal, July 2004

Orientation of myoglobin in cast multibilayer membranes of amphiphilic molecules
journal, July 1995

Directed co-assembly of hemeproteins with amphiphilic block copolymers toward functional biomolecular materials
journal, January 2011

Ferritin-Polymer Conjugates: Grafting Chemistry and Integration into Nanoscale Assemblies
journal, August 2010

Nanopatterned Protein Films Directed by Ionic Complexation with Water-Soluble Diblock Copolymers
journal, May 2012

Solid-State Nanostructured Materials from Self-Assembly of a Globular Protein–Polymer Diblock Copolymer
journal, June 2011

Highly Oriented and Ordered Arrays from Block Copolymers via Solvent Evaporation
journal, February 2004

Solvent annealing thin films of poly(isoprene-b-lactide)
journal, November 2005

Patterning with block copolymer thin films
journal, February 2005

Self-assembly of block copolymer thin films
journal, June 2010

Morphological Development in Solvent-Cast Polystyrene−Polybutadiene−Polystyrene (SBS) Triblock Copolymer Thin Films
journal, April 1998

The Full Phase Behavior for Block Copolymers in Solvents of Varying Selectivity
journal, June 2002

Nature and consequences of protein–protein interactions in high protein concentration solutions
journal, June 2008

Examination of the secondary structure of proteins by deconvolved FTIR spectra
journal, March 1986

The Use and Misuse of FTIR Spectroscopy in the Determination of Protein Structure
journal, January 1995

Approximations Leading to a Unified Exponential/Power-Law Approach to Small-Angle Scattering
journal, December 1995

Hard-sphere interactions between spherical domains in diblock copolymers
journal, September 1984

Self-Assembly and Stress Relaxation in Acrylic Triblock Copolymer Gels
journal, January 2007

Novel Chromophores and Buried Charges Control Color in mFruits ^† ^, ^‡
journal, August 2006

Single Polymer Chain Elongation of Poly( N -isopropylacrylamide) and Poly(acrylamide) by Atomic Force Microscopy
journal, November 2000

The Conformation of the Poly(ethylene glycol) Chain in Mono-PEGylated Lysozyme and Mono-PEGylated Human Growth Hormone
journal, November 2011

Phase Behavior of a Block Copolymer in Solvents of Varying Selectivity
journal, August 2000

Ordering Dynamics of a Symmetric Polystyrene- block -polyisoprene. 2. Real-Space Analysis on the Formation of Lamellar Microdomain
journal, June 1998

Block Copolymers—Designer Soft Materials
journal, February 1999

Enabling nanotechnology with self assembled block copolymer patterns
journal, October 2003

Structure and Reactivity of the Chromophore of a GFP-like Chromoprotein from Condylactis gigantea ^†
journal, June 2006

The structure of the chromophore within DsRed, a red fluorescent protein from coral
journal, October 2000

Photoactivation mechanism of PAmCherry based on crystal structures of the protein in the dark and fluorescent states
journal, November 2009

Insight into the Common Mechanism of the Chromophore Formation in the Red Fluorescent Proteins: The Elusive Blue Intermediate Revealed
journal, January 2012

Protein–excipient interactions: Mechanisms and biophysical characterization applied to protein formulation development
journal, October 2011

Fourier Transform Infrared Spectroscopic Analysis of Protein Secondary Structures
journal, August 2007

FTIR characterization of the secondary structure of proteins encapsulated within PLGA microspheres1An article of related interest has been published by Yang et al. in J. Pharm. Sci., 88(2), Feb. 1999, accepted Nov. 1998.1
journal, April 1999

Characterization of the Pressure-induced Intermediate and Unfolded State of Red-shifted Green Fluorescent Protein—A Static and Kinetic FTIR, UV/VIS and Fluorescence Spectroscopy Study
journal, July 2003

What vibrations tell about proteins
journal, November 2002

Heat Capacity of Spider Silk-Like Block Copolymers
journal, July 2011

FTIR and nDSC as Analytical Tools for High-Concentration Protein Formulations
journal, May 2006

Responsive polymers for biosensing and protein delivery
journal, January 2014

Elastin-like polypeptides as building motifs toward designing functional nanobiomaterials
journal, January 2019

Photochemistry for Well-Defined Polymers in Aqueous Media: From Fundamentals to Polymer Nanoparticles to Bioconjugates
journal, May 2018

Topological Effects on Globular Protein-ELP Fusion Block Copolymer Self-Assembly
journal, December 2014

Self-assembled nanostructures from amphiphilic globular protein–polymer hybrids
journal, September 2017

Phase transitions in concentrated solution self-assembly of globular protein–polymer block copolymers
journal, January 2013

Enzyme–Polymer Conjugates as Robust Pickering Interfacial Biocatalysts for Efficient Biotransformations and One‐Pot Cascade Reactions
journal, October 2018

Block copolymers for protein ordering
journal, January 2014

Enzyme–Polymer Conjugates as Robust Pickering Interfacial Biocatalysts for Efficient Biotransformations and One‐Pot Cascade Reactions
journal, September 2018

Self-Assembly of Globular-Protein-Containing Block Copolymers
journal, July 2013

Micellar‐Mediated Block Copolymer Ordering Dynamics Revealed by In Situ Grazing Incidence Small‐Angle X‐Ray Scattering during Spin Coating
journal, March 2019