Document Details
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Title:
CYTOCHEMICAL STUDY OF ^5-3 BETTA-HYDROXYSTERIOD DEHYDROGENASE IN TESTICULAR CELLS GROWN IN VITRO ( ENDOCRINOLOGY VOL. 79, NO. 2, AUGUST 1966, PP 406-410 )
Author(s):
STEINBERGER, E [ALBERT EINSTEIN MEDICAL CENTER, PHILADELPHIA, PA]; STEINBERGER, A [ALBERT EINSTEIN MEDICAL CENTER, PHILADELPHIA, PA]; VILAR, O [ALBERT EINSTEIN MEDICAL CENTER, PHILADELPHIA, PA]
Subject Terms:
HREX
Document Location:
Location - DOE/NNSA NUCLEAR TESTING ARCHIVE Address - P.O. Box 98521 City - Las Vegas State - NV Zip - 89193-8521 Phone - (702)794-5106 Fax - (702)862-4240 Email - NTA@NV.DOE.GOV
Document Type:
JOURNAL ARTICLE
Publication Date:
1966 Aug 31
Declassification Status:
Never classified
Document Pages:
0006
Accession Number:
NV0703824
Originating Research Org.:
ALBERT EINSTEIN MEDICAL CENTER, PHILADELPHIA, PA
OpenNet Entry Date:
1994 Aug 27
OpenNet Modified Date:
2012 May 14
Description/Abstract:
CYTOCHEMICAL REACTION FOR DELTA(5)-3 BETA-HYDROXYSTERIOD DEHYDROGENASE WAS STUDIED IN MONOLAYERS OF TESTICULAR CELLS GROWN IN VITRO. CELLS IN CULTURES ESTABLISHED FROM TESTICULAR TISSUE OF IMMATURE RATS WERE ESSENTIALLY NEGATIVE FOR DELTA(5)-3 BETA-HYDROXYSTERIOD DEHYDROGENASE. CELLS IN CULTURES ESTABLISHED FROM TESTICULAR TISSUE OF SIMILAR ANIMALS PRETREATED WITH HUMAN CHORIONIC GONADOTROPIN (HCG) WERE STRONGLY POSITIVE FOR DELTA(5)-3 BETA-HYDROXYSTEROID DEHYDROGENASE. ADDITION OF HCG TO THE CULTURE MEDIUM FAILED TO INDUCE A POSITIVE REACTION FOR THIS ENZYME. THESE STUDIES SHOW THAT TESTICULAR CELLS WHICH MAY BE ACTIVE IN STEROID BIOSYNTHESIS CAN BE GROWN IN VITRO.