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EXTRAVASCULAR AND INTRATUBULAR DIFFUSION OF BABELED SERUM PROTIENS IN RAT TESTIS ( JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY, VOL 13, NO. 5, 1964, PP 376 -385 )

ALVAREZ, B; MANCINI, R E; SEIGUER, A C; VILAR, O

HREX

Location - DOE/NNSA NUCLEAR TESTING ARCHIVE Address - P.O. Box 98521 City - Las Vegas State - NV Zip - 89193-8521 Phone - (702)794-5106 Fax - (702)862-4240 Email - NTA@NV.DOE.GOV

JOURNAL ARTICLE

1964 Dec 17

Never classified

0010

NV0703823

1994 Aug 27

2012 May 14

RAT WHOLE SERUM, ALBUMIN, GLOBULINS, AND FIBRINOGEN WERE LABELED WITH FLUORESCENT DYES; ALBUMIN WAS ALSO TAGGED WITH RADIOACTIVE IODINE AND TRITIUM. IN ADDITION HETEROLOGOUS ALBUMIN WAS ALSO LABELED WITH FLUORESCENT DYES AND RADIOIODINE. THE PROTEINS WERE INTRAVENOUSLY INJECTED IN PREPUBERTAL, PUBERTAL AND ADULT RATS, AND THEIR DECAY IN THE CIRCULATION AND HISTOLOGICAL DISTRIBUTION IN THE TESTIS AND EPIDIDYMIS WAS STUDIED. AS CONTROLS OTHER ANIMALS WERE SIMILARLY INJECTED WITH FREE LABELS ALONE AND WITH LABELED DENATURED AND DEGRADED ALBUMIN; ALSO UNLABELED HOMOLOGOUS AND HETEROLOGOUS ALBUMINS WERE ADMINISTERED FOLLOWED BY INCUBATION OF BOTH ORGANS WITH CORRESPONDING LABELED ANTISERA APPLYING THE COON'S TECHNIQUE. IT WAS OBSERVED: 1) WITH THE EXCEPTION OF FIBRINOGEN, LABELED SERUM PROTEINS RAPIDLY APPEARED IN THE LUMINA OF VESSELS, DIFFUSED EXTRAVASCULARLY IN THE INTERTUBULAR SPACES AND FINALLY ARRIVED IN BETWEEN THE GERMINAL CELLS AND IN THE LUMINA OF SEMINIFEROUS TUBULES. ALSO LABELED MATERIAL WAS PRESENT IN THE LUMEN OF EPIDIDYMAL CANALICULI BUT NOT IN THE DUCTUS DEFERENS. 2) THIS EXTRAVASCULAR AND INTRATUBULAR DIFFUSION WAS PARALLEL TO THE FAST COMPONENT OF THE TIME DECLINE CURVE LABELED HOMOLOGOUS SERUM PROTEINS IN THE CIRCULATION. 3) THERE WAS NO GREAT DIFFERENCE BETWEEN YOUNG AND ADULT RATS IN THE EXTRAVASCULAR DIFFUSION PROCESS, BUT INTRATUBULAR PASSAGE WAS HIGHER IN PUBERTAL AND ADULT ANIMALS. 4) CONTROL EXPERIMENTS REVEALED THE PRESENCE OF SOME FLUORESCENT MATERIAL ONLY IN THE VESSELS AND MACROPHAGES, WHEREAS THE IMMUNOFLUORESCENT TECHNIQUE SHOWED A VERY SIMILAR LOCALIZATION OF UNLABELED PROTEINS TO THAT PROVIDED BY THE INJECTION OF DIRECTLY LABELED PROTEINS.