Document Details

(844 K)

INCORPORATION BY THE KIDNEY OF FLUORESCENT PITUITARY HORMONES ( JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY, VOL. 12, NO. 8, AUGUST 1964, PP 621-627 )

ALVAREZ, B; DAVIDSON, O; MANCINI, R E; VILAR, O

HREX

Location - DOE/NNSA NUCLEAR TESTING ARCHIVE Address - P.O. Box 98521 City - Las Vegas State - NV Zip - 89193-8521 Phone - (702)794-5106 Fax - (702)862-4240 Email - NTA@NV.DOE.GOV

JOURNAL ARTICLE

1964 Feb 05

Never classified

0007

NV0703822

1994 Aug 27

2012 May 14

SEVERAL ARMOUR AND NIH PITUITARY HORMONE PREPARATIONS AS WELL AS OTHER HOMOLOGOUS SERUM PROTEIN FRACTIONS WERE LABELED WITH FLUORESCENT DYES. RATS WERE INJECTED INTRAVENOUSLY WITH THE DIFFERENT LABELED PROTEINS AND THE ANIMALS KILLED AT VARIOUS INTERVALS FROM 3 MINUTES TO 10 DAYS. SECTIONS OF THE KIDNEYS WERE STUDIED UNDER ULTRAVIOLET LIGHT. IT WAS OBSERVED THAT ALL THE LABELED PROTEINS PASSED THE GLOMERULAR FILTER AND WERE ACCUMULATED BY THE CELLS OF THE PROXIMAL CONVOLUTED TUBULES. LABELED PITUITARY HORMONES WERE, IN ADDITION, REABSORBED BY THE THIN LIMB AND COLLECTING TUBULES AND APPEARED IN THE LUMEN OF BLADDER. ALL THE PROTEINS WERE REABSORBED WITH THE SAME SEQUENCE, BUT PITUITARY HORMONES APPEARED FASTER AND IN LARGER AMOUNTS IN THE SAME SITES OF THE NEPHRON. THEY WERE ALSO DETECTED IN THE LUMEN OF THE URETER AND IN THE BLADDER. ALTHOUGH THE DIFFERENT HORMONES SHOWED A PREFERENTIAL ACCUMULATION IN THE VESSELS OF THE CORRESPONDING TARGET GLANDS, THEY BEHAVE SIMILARLY IN THE KIDNEY.