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Title:
ELECRON MIRCOSCOPY OF ISOLATED RAT TESTICULAR CELLS GROWN IN VITRO ( ZEITSCHRIFT FUR ZELLFORSCHUNG 74, PP 529-538, 1966 )
Author(s):
STEINBBERGER, A [ALBERT EINSTEIN MEDICAL CENTER, PHILADELPHIA, PA]; STEINBERGER, E [ALBERT EINSTEIN MEDICAL CENTER, PHILADELPHIA, PA]; VILAR, O [ALBERT EINSTEIN MEDICAL CENTER, PHILADELPHIA, PA]
Subject Terms:
HREX
Document Location:
Location - DOE/NNSA NUCLEAR TESTING ARCHIVE Address - P.O. Box 98521 City - Las Vegas State - NV Zip - 89193-8521 Phone - (702)794-5106 Fax - (702)862-4240 Email - NTA@NV.DOE.GOV
Document Type:
JOURNAL ARTICLE
Publication Date:
1966 May 01
Declassification Status:
Never classified
Document Pages:
0010
Accession Number:
NV0703820
Originating Research Org.:
ALBERT EINSTEIN MEDICAL CENTER, PHILADELPHIA, PA
OpenNet Entry Date:
1994 Aug 27
OpenNet Modified Date:
2012 May 14
Description/Abstract:
SUSPENSIONS OF VIABLE TESTICULAR CELLS, OBTAINED FROM TWO GROUPS OF RATS WERE CULTURED FOR TEN DAYS. NUMEROUS CELLS ADHERED TO THE GLASS TO FORM A CONFLUENT MONOLAYER AND REMAINED IN GOOD CONDITION AFTER TEN DAYS. THIS MONOLAYER CONTAINED TWO CELL TYPES IDENTIFIED BY ELECTRON MICROSCOPY: FIBROBLASTIC CELLS AND LEYDIG CELLS. THE RELATIVE PROPORTION BY WHICH THESE TWO CELLS CONTRIBUTED TO THE MONOLAYER WAS RELATED TO THE CONDITION OF THE DONOR WHEN THE CULTURE WAS INITIATED. FIBROBLASTIC CELLS WERE MORE ABUNDANT WHEN THE ANIMAL WAS NOT PREVIOUSLY TREATED WITH HCG. HOWEVER, LEYDIG CELLS ALMOST EXCLUSIVELY FORMED THE MONOLAYER WHEN CULTURES WERE BEGUN WITH TESTICULAR CELLS OF HCG-TREATED RATS. GONADOTROPINS, ON THE OTHER HAND, DID NOT SEEM CAPABLE OF ACTING IN VITRO.