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Title:
AN ELECTRON MICROSCOPIC STUDY OF CULTURED RAT TESTICULAR FRAGMENTS ( ZEITSCHRIFT FUR ZELLFORSCHUNG 78, PP 221-233 (1967) )
Author(s):
STEINBERGER, A [ALBERT EINSTEIN MEDICAL CENTER, PHILADELPHIA, PA]; STEINBERGER, E [ALBERT EINSTEIN MEDICAL CENTER, PHILADELPHIA, PA]; VILAR, O [ALBERT EINSTEIN MEDICAL CENTER, PHILADELPHIA, PA]
Subject Terms:
HREX
Document Location:
Location - DOE/NNSA NUCLEAR TESTING ARCHIVE Address - P.O. Box 98521 City - Las Vegas State - NV Zip - 89193-8521 Phone - (702)794-5106 Fax - (702)862-4240 Email - NTA@NV.DOE.GOV
Document Type:
JOURNAL ARTICLE
Publication Date:
1967 Dec 31
Declassification Status:
Never classified
Document Pages:
0001
Accession Number:
NV0703812
Originating Research Org.:
ALBERT EINSTEIN MEDICAL CENTER, PHILADELPHIA, PA
OpenNet Entry Date:
1994 Aug 27
OpenNet Modified Date:
2012 May 14
Description/Abstract:
FRAGMENTS OF TESTICULAR TISSUE OF 26-DAY-OLD RATS WERE GROWN AS ORGAN CULTURES DURING ONE TO SIX WEEKS. ELECTRON MICROSCOPE STUDIES SHOWED THAT THESE TISSUES CAN BE MAINTAINED IN VITRO FOR PROLONGED PERIODS OF TIME, ALTHOUGH THE MOST DIFFERENTIATED ELEMENTS (LEYDIG AND SPERMATIC CELLS OTHER THAN SPERMATOGONIA) FAIL TO CONTINUE THEIR DEVELOPMENT AND DEGENERATE RATHER RAPIDLY. THE CONNECTIVE TISSUE STRUCTURES PRESERVE THEIR USUAL ARCHITECTURE, BUT THE BASAL MEMBRANE OF THE TUBULES APPEARS EXTREMELY FOLDED AND DETACHED FROM THE EPITHELIUM. AFTER FOUR WEEKS IN CULTURE, SPERMATOGONIA WITHOUT DIFFERENTIATING ARE STILL PRESENT, AND AMONG THEM THE PRESENCE OF A "MORE PRIMITIVE" TYPE IS NOTED. SERTOLI CELLS ARE WELL PRESERVED AND ULTRASTRUCTURALLY THEY PRESENT THE CHARACTERISTICS OF THE ADULT TYPE. THE POSSIBILITY EXISTS THAT DIFFERENTIATION OF THESE TWO LINES OF CELLS MAY BE ACHIEVED IN VITRO IF THE FACTORS NECESSARY FOR THEIR GROWTH AND DIFFERENTIATION ARE RECOGNIZED AND INCORPORATED IN THE CULTURE SYSTEM.