Document Details


Title:
The Fluoresometer
Author(s):
Subject Terms:
fluorescence methods; instrument; uranium ion, ultraviolet
Document Location:
DOE INFORMATION CENTER 1 Science.gov Way, Oak Ridge, TN 37831; Eva Butler; Phone: 865-241-4780; Toll-Free: 800-382-6938, Option 6; FAX: 865-574-3521; Email: doeic@oro.doe.gov
Document Categories:
Science and Technology\Instruments and Equipment; Health, Safety and Environment\Worker Health and Safety
Document Type:
REPORT
Publication Date:
1946 Jan 29
Declassification Date:
1956 Mar 28
Declassification Status:
Declassified
Document Pages:
28
Accession Number:
ORF18500
Document Number(s):
Y-407
Originating Research Org.:
Carbide and Carbon Chemicals Corporation
OpenNet Entry Date:
1998 Jun 16
Description/Abstract:
This report consists of information concerning the fluoresometer Model 2B instrument which has been in routine use for a period of six months and has given very satisfactory results. This instrument has been used for analysis of urine samples to detect uranium collected under rigid conditions of cleanliness from process workers. The sample is fused in a cake of sodium fluoride and is activated with ultraviolet light from a G. E. S4 sunlamp. The uranium ion acts as an activator to cause certain inorganic crystalline materials such as sodium fluoride to fluoresce brightly. The fluorescent intensity is a function of the amount of uranium present in the crystal. This technique provides a highly sensitive method of analysis for measuring small amounts of uranium. It was noted that an excessive amount of sodium fluoride used will quench (suppress) the fluorescence to insignificant amounts. This report describes the fluoresometer instrument, discusses sample preparation and instrument operation procedures, other activating elements, the effects of quenchers, and methods of reducing errors due to quenching. Also discussed are the calibration technique and curves, use of standard fluorescent material for daily calibration, and care and servicing of the instrument. A sample calibration chart, a picture and wiring diagram for the fluoresometer, a parts list, and references are included in this report.


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