National Library of Energy BETA

Sample records for orthopedic maternity ear

  1. Bioactive glass coatings for orthopedic metallic implants

    SciTech Connect (OSTI)

    Lopez-Esteban, Sonia; Saiz, Eduardo; Fujino, Sigheru; Oku, Takeo; Suganuma, Katsuaki; Tomsia, Antoni P.


    The objective of this work is to develop bioactive glass coatings for metallic orthopedic implants. A new family of glasses in the SiO2-Na2O-K2O-CaO-MgO-P2O5 system has been synthesized and characterized. The glass properties (thermal expansion, softening and transformation temperatures, density and hardness) are in line with the predictions of established empirical models. The optimized firing conditions to fabricate coatings on Ti-based and Co-Cr alloys have been determined and related to the glass properties and the interfacial reactions. Excellent adhesion to alloys has been achieved through the formation of 100-200 nm thick interfacial layers (Ti5Si3 on Ti-based alloys and CrOx on Co-Cr). Finally, glass coatings, approximately 100 mu m thick, have been fabricated onto commercial Ti alloy-based dental implants.

  2. In-vivo orthopedic implant diagnostic device for sensing load, wear, and infection

    DOE Patents [OSTI]

    Evans, III, Boyd McCutchen; Thundat, Thomas G.; Komistek, Richard D.; Dennis, Douglas A.; Mahfouz, Mohamed


    A device for providing in vivo diagnostics of loads, wear, and infection in orthopedic implants having at least one load sensor associated with the implant, at least one temperature sensor associated with the implant, at least one vibration sensor associated with the implant, and at least one signal processing device operatively coupled with the sensors. The signal processing device is operable to receive the output signal from the sensors and transmit a signal corresponding with the output signal.

  3. Systems and methods for biometric identification using the acoustic properties of the ear canal

    DOE Patents [OSTI]

    Bouchard, A.M.; Osbourn, G.C.


    The present invention teaches systems and methods for verifying or recognizing a person`s identity based on measurements of the acoustic response of the individual`s ear canal. The system comprises an acoustic emission device, which emits an acoustic source signal s(t), designated by a computer, into the ear canal of an individual, and an acoustic response detection device, which detects the acoustic response signal f(t). A computer digitizes the response (detected) signal f(t) and stores the data. Computer-implemented algorithms analyze the response signal f(t) to produce ear-canal feature data. The ear-canal feature data obtained during enrollment is stored on the computer, or some other recording medium, to compare the enrollment data with ear-canal feature data produced in a subsequent access attempt, to determine if the individual has previously been enrolled. The system can also be adapted for remote access applications. 5 figs.

  4. Systems and methods for biometric identification using the acoustic properties of the ear canal

    DOE Patents [OSTI]

    Bouchard, Ann Marie; Osbourn, Gordon Cecil


    The present invention teaches systems and methods for verifying or recognizing a person's identity based on measurements of the acoustic response of the individual's ear canal. The system comprises an acoustic emission device, which emits an acoustic source signal s(t), designated by a computer, into the ear canal of an individual, and an acoustic response detection device, which detects the acoustic response signal f(t). A computer digitizes the response (detected) signal f(t) and stores the data. Computer-implemented algorithms analyze the response signal f(t) to produce ear-canal feature data. The ear-canal feature data obtained during enrollment is stored on the computer, or some other recording medium, to compare the enrollment data with ear-canal feature data produced in a subsequent access attempt, to determine if the individual has previously been enrolled. The system can also be adapted for remote access applications.

  5. Finite-element analysis of earing using non-quadratic yield surfaces

    SciTech Connect (OSTI)

    Logan, R.W.


    During deep draw cupping, the phenomenon known as earing may occur as the cup wall is formed, resulting in a periodic variation of cup wall height around the perimeter of the finished cup. This is generally due to planar anisotropy of flow in rolled sheet product. It is generally observed that the anisotropy parameter R will vary in the plane of the sheet when ears are observed in cupping, with a parameter {Delta}R describing the variation of R in the plane of the sheet. For many common textures in face-centered and body-centered materials, the ears form relative to the sheet rolling direction at 0{degrees} and 90{degrees} around the perimeter if {Delta}R>0, and at -45{degrees} and +45{degrees} if {Delta}R<0. There is extensive experimental evidence that ear height shows a linear correlation with {Delta}R/R, but attempts to duplicate this using the finite-element method are highly dependent on both the methodology and yield surface used. It was shown previously that using a coarse mesh and the quadratic Hill yield surface tends to greatly under predict earing. In this study, we have used two different finite-element codes developed at LLNL to examine the predicted earing using both quadratic Hill and alternative non-quadratic yield surfaces. These results are compared to experimental data and conclusions drawn about the most desirable closed-form yield surfaces to duplicate the observed earing phenomena.

  6. Sulfur mustard induces an endoplasmic reticulum stress response in the mouse ear vesicant model

    SciTech Connect (OSTI)

    Chang, Yoke-Chen; Wang, James D.; Svoboda, Kathy K.; Casillas, Robert P.; Laskin, Jeffrey D.; Gordon, Marion K.; Gerecke, Donald R.


    The endoplasmic reticulum (ER) stress response is a cell survival pathway upregulated when cells are under severe stress. Severely damaged mouse ear skin exposed to the vesicant, sulfur mustard (bis-2-chloroethyl sulfide, SM), resulted in increased expression of ER chaperone proteins that accompany misfolded and incorrectly made proteins targeted for degradation. Time course studies with SM using the mouse ear vesicant model (MEVM) showed progressive histopathologic changes including edema, separation of the epidermis from the dermis, persistent inflammation, upregulation of laminin ?2 (one of the chains of laminin-332, a heterotrimeric skin glycoprotein required for wound repair), and delayed wound healing from 24 h to 168 h post exposure. This was associated with time related increased expression of the cell survival ER stress marker, GRP78/BiP, and the ER stress apoptosis marker, GADD153/CHOP, suggesting simultaneous activation of both cell survival and non-mitochondrial apoptosis pathways. Dual immunofluorescence labeling of a keratinocyte migration promoting protein, laminin ?2 and GRP78/BIP, showed colocalization of the two molecules 72 h post exposure indicating that the laminin ?2 was misfolded after SM exposure and trapped within the ER. Taken together, these data show that ER stress is induced in mouse skin within 24 h of vesicant exposure in a defensive response to promote cell survival; however, it appears that this response is rapidly overwhelmed by the apoptotic pathway as a consequence of severe SM-induced injury. - Highlights: ? We demonstrated ER stress response in the mouse ear vesicant model. ? We described the asymmetrical nature of wound repair in the MEVM. ? We identified the distribution of various ER stress markers in the MEVM.

  7. A comparative study of the inner ear structures of artiodactyls and early cetaceans

    SciTech Connect (OSTI)

    Klingshirn, M.A.; Luo, Z.


    It has been suggested that the order Cetacea (whales and porpoises) are closely related to artiodactyls, even-hoofed ungulate mammals such as the pig and cow. Paleontological and molecular data strongly supports this concept of phylogenetic relationships. In a study of DNA sequences of two mitochondrial ribosomal gene segments of cetaceans, the artiodactyls were found to be closest related to Cetaceans. These well accepted studies on the phylogenetic affinities of artiodactyls and cetaceans cause us to conduct a comparative study of the bony structure of the inner ear of these two taxa.

  8. The distribution and contaminant exposure of Rafinesque's Big-Eared Bats in South Carolina with an emphasis on bridge surveys.

    SciTech Connect (OSTI)

    F.M. Bennett; S.C. Loeb; W.W. Bowerman


    Rafinesque's big-eared bat (Corynorhinus rafinesquii), an insectivorous mammal indigenous to the southern United States, has long been referred to as one of the least known bats in North America. Although there has been a moderate increase in the number of peer-reviewed articles published on this species in the past 6 years, the basic ecology and status of Rafinesque's big-eared bat remains largely obscure. Prior to 1996, when the United States Fish and Wildlife Service (USFWS) discontinued the list of Candidate Species, Rafinesque's big-eared bat was listed as a Federal Category 2 Candidate species. Currently, Rafinesque's big-eared bat is recognized as a ''species of special concern'' across most of its range but receives no legal protection. Nonetheless, the USFWS and numerous state agencies remain concerned about this species. Further biological research and field study are needed to resolve the conservation status of this taxona. In response to the paucity of information regarding the status and distribution of Rafinesque's big-eared bat, statewide survey of highway bridges used as roost sites was conducted.

  9. Seasonal and multiannual roost use by Rafinesque's Big-eared Bats in the Coastal Plain of South Carolina.

    SciTech Connect (OSTI)

    Loeb, Susan, C.; Zarnoch, Stanley, J.


    Little is known about factors affecting year-round use of roosts by Rafinesque's big-eared bats (Corynorhinus rafinesquii) or the long-term fidelity of this species to anthropogenic or natural roosts. The objectives of this study were to test whether seasonal use of roosts by Rafinesque's big-eared bats varied with roost type and environmental conditions within and among seasons and to document multiannual use of natural and anthropogenic structures by this species. We inspected 4 bridges, 1 building, and 59 tree roosts possessing basal cavity openings; roosts were inspected at least once per week from May through October in every year from 2005 through 2008 and once a month from November through April in every year from 2005 through 2009. We found that use of anthropogenic roosts was significantly greater than the use of tree roosts in summer but that the use of structure types did not differ in other seasons. There was significant seasonal variation in use of anthropogenic and tree roosts. Anthropogenic roost use was higher in summer than in all other seasons. There was no significant difference in tree use among spring, summer, and fall, but use in winter was significantly lower in 2 years of the study. Overall use of anthropogenic and tree roosts was positively related to minimum temperature, but the relationship between use of roosts and minimum temperature varied among seasons. Bats showed multiannual fidelity ({ge} 4 years) to all anthropogenic roosts and to some tree roosts, but fidelity of bats to anthropogenic roosts was greater and more consistent than to tree roosts. Our data indicate that Rafinesque's big-eared bats responded differently to environmental conditions among seasons; thus, a variety of structure types and characteristics are necessary for conservation of these bats. We suggest long-term protection of roost structures of all types is necessary for conservation of Rafinesque's big-eared bats in the southeast Coastal Plain.

  10. Maternal blood metal levels and fetal markers of metabolic function

    SciTech Connect (OSTI)

    Ashley-Martin, Jillian; Dodds, Linda; Arbuckle, Tye E.; Ettinger, Adrienne S.; Shapiro, Gabriel D.; Fisher, Mandy; Taback, Shayne; Bouchard, Maryse F.; Monnier, Patricia; Dallaire, Renee; Fraser, William D.


    Exposure to metals commonly found in the environment has been hypothesized to be associated with measures of fetal growth but the epidemiological literature is limited. The Maternal–Infant Research on Environmental Chemicals (MIREC) study recruited 2001 women during the first trimester of pregnancy from 10 Canadian sites. Our objective was to assess the association between prenatal exposure to metals (lead, arsenic, cadmium, and mercury) and fetal metabolic function. Average maternal metal concentrations in 1st and 3rd trimester blood samples were used to represent prenatal metals exposure. Leptin and adiponectin were measured in 1363 cord blood samples and served as markers of fetal metabolic function. Polytomous logistic regression models were used to estimate odds ratios (OR) and 95% confidence intervals (CI) for the association between metals and both high (≥90%) and low (≤10%) fetal adiponectin and leptin levels. Leptin levels were significantly higher in female infants compared to males. A significant relationship between maternal blood cadmium and odds of high leptin was observed among males but not females in adjusted models. When adjusting for birth weight z-score, lead was associated with an increased odd of high leptin. No other significant associations were found at the top or bottom 10th percentile in either leptin or adiponectin models. This study supports the proposition that maternal levels of cadmium influence cord blood adipokine levels in a sex-dependent manner. Further investigation is required to confirm these findings and to determine how such findings at birth will translate into childhood anthropometric measures. - Highlights: • We determined relationships between maternal metal levels and cord blood adipokines. • Cord blood leptin levels were higher among female than male infants. • Maternal cadmium was associated with elevated leptin in male, not female infants. • No significant associations were observed between metals and

  11. SU-E-I-63: Quantitative Evaluation of the Effects of Orthopedic Metal Artifact Reduction (OMAR) Software On CT Images for Radiotherapy Simulation

    SciTech Connect (OSTI)

    Jani, S [Sharp Memorial Hospital, San Diego, CA (United States)


    Purpose: CT simulation for patients with metal implants can often be challenging due to artifacts that obscure tumor/target delineation and normal organ definition. Our objective was to evaluate the effectiveness of Orthopedic Metal Artifact Reduction (OMAR), a commercially available software, in reducing metal-induced artifacts and its effect on computed dose during treatment planning. Methods: CT images of water surrounding metallic cylindrical rods made of aluminum, copper and iron were studied in terms of Hounsfield Units (HU) spread. Metal-induced artifacts were characterized in terms of HU/Volume Histogram (HVH) using the Pinnacle treatment planning system. Effects of OMAR on enhancing our ability to delineate organs on CT and subsequent dose computation were examined in nine (9) patients with hip implants and two (2) patients with breast tissue expanders. Results: Our study characterized water at 1000 HU with a standard deviation (SD) of about 20 HU. The HVHs allowed us to evaluate how the presence of metal changed the HU spread. For example, introducing a 2.54 cm diameter copper rod in water increased the SD in HU of the surrounding water from 20 to 209, representing an increase in artifacts. Subsequent use of OMAR brought the SD down to 78. Aluminum produced least artifacts whereas Iron showed largest amount of artifacts. In general, an increase in kVp and mA during CT scanning showed better effectiveness of OMAR in reducing artifacts. Our dose analysis showed that some isodose contours shifted by several mm with OMAR but infrequently and were nonsignificant in planning process. Computed volumes of various dose levels showed <2% change. Conclusions: In our experience, OMAR software greatly reduced the metal-induced CT artifacts for the majority of patients with implants, thereby improving our ability to delineate tumor and surrounding organs. OMAR had a clinically negligible effect on computed dose within tissues. Partially funded by unrestricted

  12. SU-E-I-75: Evaluation of An Orthopedic Metal Artifact Reduction (O-MAR) Algorithm On Patients with Spinal Prostheses Near Spinal Tumors

    SciTech Connect (OSTI)

    Shen, Z; Xia, P; Djemil, T; Klahr, P


    Purpose: To evaluate the impact of a commercial orthopedic metal artifact reduction (O-MAR) algorithm on CT image quality and dose calculation for patients with spinal prostheses near spinal tumors. Methods: A CT electron density phantom was scanned twice: with tissue-simulating inserts only, and with a titanium insert replacing solid water. A patient plan was mapped to the phantom images in two ways: with the titanium inside or outside of the spinal tumor. Pinnacle and Eclipse were used to evaluate the dosimetric effects of O-MAR on 12-bit and 16-bit CT data, respectively. CT images from five patients with spinal prostheses were reconstructed with and without O-MAR. Two observers assessed the image quality improvement from O-MAR. Both pencil beam and Monte Carlo dose calculation in iPlan were used for the patient study. The percentage differences between non-OMAR and O-MAR datasets were calculated for PTV-min, PTV-max, PTV-mean, PTV-V100, PTV-D90, OAR-V10Gy, OAR-max, and OAR-D0.1cc. Results: O-MAR improved image quality but did not significantly affect the dose distributions and DVHs for both 12-bit and 16- bit CT phantom data. All five patient cases demonstrated some degree of image quality improvement from O-MAR, ranging from small to large metal artifact reduction. For pencil beam, the largest discrepancy was observed for OARV-10Gy at 5.4%, while the other seven parameters were ≤0.6%. For Monte Carlo, the differences between non-O-MAR and O-MAR datasets were ≤3.0%. Conclusion: Both phantom and patient studies indicated that O-MAR can substantially reduce metal artifacts on CT images, allowing better visualization of the anatomical structures and metal objects. The dosimetric impact of O-MAR was insignificant regardless of the metal location, image bit-depth, and dose calculation algorithm. O-MAR corrected images are recommended for radiation treatment planning on patients with spinal prostheses because of the improved image quality and no need to modify

  13. Preconception maternal polychlorinated biphenyl concentrations and the secondary sex ratio

    SciTech Connect (OSTI)

    Taylor, Kira C. [Division of Epidemiology, Statistics and Prevention Research, National Institutes of Child Health and Development (NICHD), NIH, DHHS, 6100 Executive Blvd, Room 7B03, Rockville, MD 20852 (United States); Department of Epidemiology, 1518 Clifton Road, NE Atlanta, GA 30322 (United States); Jackson, Leila W. [Department of Epidemiology and Biostatistics, WG37, Case Western Reserve University School of Medicine, 10900 Euclid Avenue Cleveland, OH 44106-4945 (United States); Lynch, Courtney D. [Division of Epidemiology, Statistics and Prevention Research, National Institutes of Child Health and Development (NICHD), NIH, DHHS, 6100 Executive Blvd, Room 7B03, Rockville, MD 20852 (United States); Kostyniak, Paul J. [Toxicology Research Center, 134 Cary Hall, University at Buffalo, State of New York, 3434 Main St., Buffalo, NY 14214-3000 (United States); Buck Louis, Germaine M. [Division of Epidemiology, Statistics and Prevention Research, National Institutes of Child Health and Development (NICHD), NIH, DHHS, 6100 Executive Blvd, Room 7B03, Rockville, MD 20852 (United States)]. E-mail:


    The secondary sex ratio is the ratio of male to female live births and historically has ranged from 102 to 106 males to 100 females. Temporal declines have been reported in many countries prompting authors to hypothesize an environmental etiology. Blood specimens were obtained from 99 women aged 24-34 prior to attempting pregnancy and quantified for 76 polychlorinated biphenyl (PCB) congeners using dual column gas chromatography with electron capture detection. Women were prospectively followed until pregnancy or 12 cycles of trying. The odds of a male birth for three PCB groupings (total, estrogenic, anti-estrogenic) controlling for maternal characteristics were estimated using logistic regression. Among the 50 women with live births and PCB data, 26 female and 24 male infants were born (ratio 0.92). After adjusting for age and body mass index, odds of a male birth were elevated among women in the second (OR=1.29) and third (OR=1.48) tertiles of estrogenic PCBs; odds (OR=0.70) were reduced among women in the highest tertile of anti-estrogenic PCBs. All confidence intervals included one. The direction of the odds ratios in this preliminary study varied by PCB groupings, supporting the need to study specific PCB patterns when assessing environmental influences on the secondary sex ratio.

  14. Gestational urinary bisphenol A and maternal and newborn thyroid hormone concentrations: The HOME Study

    SciTech Connect (OSTI)

    Romano, Megan E.; Webster, Glenys M.; Vuong, Ann M.; Thomas Zoeller, R.; Chen, Aimin; Hoofnagle, Andrew N.; Calafat, Antonia M.; Karagas, Margaret R.; Yolton, Kimberly; Lanphear, Bruce P.; Braun, Joseph M.


    Bisphenol A (BPA), an endocrine disruptor used in consumer products, may perturb thyroid function. Prenatal BPA exposure may have sex-specific effects on thyroid hormones (THs). Our objectives were to investigate whether maternal urinary BPA concentrations during pregnancy were associated with THs in maternal or cord serum, and whether these associations differed by newborn sex or maternal iodine status. We measured urinary BPA concentrations at 16 and 26 weeks gestation among pregnant women in the HOME Study (2003–2006, Cincinnati, Ohio). Thyroid stimulating hormone (TSH) and free and total thyroxine (T{sub 4}) and triiodothyronine (T{sub 3}) were measured in maternal serum at 16 weeks (n=181) and cord serum at delivery (n=249). Associations between BPA concentrations and maternal or cord serum TH levels were estimated by multivariable linear regression. Mean maternal urinary BPA was not associated with cord THs in all newborns, but a 10-fold increase in mean BPA was associated with lower cord TSH in girls (percent change=−36.0%; 95% confidence interval (CI): −58.4, −1.7%), but not boys (7.8%; 95% CI: −28.5, 62.7%; p-for-effect modification=0.09). We observed no significant associations between 16-week BPA and THs in maternal or cord serum, but 26-week maternal BPA was inversely associated with TSH in girls (−42.9%; 95% CI: −59.9, −18.5%), but not boys (7.6%; 95% CI: −17.3, 40.2%; p-for-effect modification=0.005) at birth. The inverse BPA–TSH relation among girls was stronger, but less precise, among iodine deficient versus sufficient mothers. Prenatal BPA exposure may reduce TSH among newborn girls, particularly when exposure occurs later in gestation. - Highlights: • Examined associations of BPA with thyroid hormones in pregnant women and newborns. • Assessed effect modification of BPA–thyroid hormone associations by newborn sex. • Greater BPA related to decreased thyroid stimulating hormone in girls' cord serum. • Results may

  15. QER- Comment of EAR [DOE]

    I would first like to say that the North East does not have an energy supply problem. The problem that we have is a demand/supply issue. If the problem is only referred to as a supply problem then only supply solutions will be looked at. When we talk about meeting our energy needs the order should be: 1st Conservation 2nd Energy Efficiency …..and if we have a concern about Climate Change 3rd Renewables 4th Polluting technologies such as Fossil Fuels and Nuclear If we do not implement this list in the above order we will leave a very different climate and a planet that is difficult to live on for our children. Conservation, Efficiency and Renewables have been mentioned by a few of the presenters today. We need to bring Conservation, Efficiency and Renewables to center stage to solve our energy needs. Recommendations: Institute to a greater extent "Peak Load Shaving". Make it mandatory that all buildings that are built are Zero Net Energy or Zero Plus Energy. Institute a more aggressive "Time of Use" Electric and Gas Metering System that Mr Reilly of the Vermont Electric Power Company mentioned earlier. Put a severe Carbon Tax on all polluting Energy Producers. Measure CO2 emissions for the complete cycle of extraction, transportation, storage and burning of all Energy options. Remove all incentives and support for Energy Producers of Fossil Fuel and Nuclear.

  16. Effects of Love Canal soil extracts on maternal health and fetal development in rats

    SciTech Connect (OSTI)

    Silkworth, J.B.; Tumasonis, C.; Briggs, R.G.; Narang, A.S.; Narang, R.S.; Rej, R.; Stein, V.; McMartin, D.N.; Kaminsky, L.S.


    The effects of a solvent extract of the surface soil of the Love Canal chemical dump site, Niagara Falls, New York, and of a natural extract, or leachate, which is drained from the canal for treatment, on the maternal health and fetal development were determined in rats. The solvent extract, which was contaminated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (2, 3,7,8-TCDD) at 170 ppb and numerous other chlorinated organic compounds with the primary identified components being the isomers of benzenehexachloride (BHC), was dissolved in corn oil and administered by gavage to pregnant rats at 0,25,75, or 150 mg crude extract/kg/day on Days 6-15 of gestation. A 67% mortality was observed at the highest dose. The rats were sacrificed on Day 20. Dose-related increases in relative liver weight accompanied by hepatocyte hypertrophy were observed at all dose levels. Fetal birthweight was decreased at 75 and 150 mg extract/kg/day. No major treatment-related soft tissue or skeletal malformations, except for delayed ossification, were observed. Based on literature values for BHC, all of the observed toxicity could be accounted for by the BHC contaminants of the extract. The crude organic phase of the leachate was administered to pregnant rats at 0,10,100, or 250 mg/kg/day as described above. Maternal weight gain decreased at 100 and 250 mg/kg/day, accompanied by 5 and 14% maternal mortality, and 1 and 3 dead fetuses, respectively. Early resorptions and the percentage of dead implants increased whereas fetal birthweights were decreased at 250 mg/kg/day. No major treatment-related soft tissue or skeletal malformations, except for delayed ossification, were observed. The primary components of the complex leachate by mass were tetrachloroethanes; however, 2,3,7,8-TCDD, which was present at 3 ppm, probably accounted for all the observed toxicity.

  17. Concurrent determination of bisphenol A pharmacokinetics in maternal and fetal rhesus monkeys

    SciTech Connect (OSTI)

    Patterson, Tucker A.; Twaddle, Nathan C.; Roegge, Cindy S.; Callicott, Ralph J.; Fisher, Jeffrey W.; Doerge, Daniel R.


    Bisphenol A (BPA) is an important industrial chemical used as the monomer for polycarbonate plastic and in epoxy resins for food can liners. Worldwide biomonitoring studies consistently find a high prevalence of BPA conjugates in urine (> 90%) in amounts consistent with aggregate exposure at levels below 1 ?g/kg bw/d. The current study used LC/MS/MS to measure concurrently the pharmacokinetics of aglycone (active) and conjugated (inactive) deuterated BPA (d6) in maternal and fetal rhesus monkey serum, amniotic fluid, and placenta following intravenous injection in the dam (100 ?g/kg bw). Internal exposures of the fetus to aglycone d6-BPA (serum AUC) were attenuated by maternal, placental, and fetal Phase II metabolism to less than half that in the dam. Levels of aglycone and conjugated d6-BPA measured in whole placenta were consistent with a role in metabolic detoxification. The monotonic elimination of aglycone d6-BPA from the fetal compartment accompanied by persistent conjugate levels provides further evidence arguing against the hypothesis that BPA conjugates are selectively deconjugated by either the placenta or fetus. These results also provide benchmarks to guide the interpretation of human cord blood, amniotic fluid, and placenta sampling and measurement strategies as a basis for estimating fetal exposures to BPA. This study in a non-human primate model provides additional pharmacokinetic data for use in PBPK modeling of perinatal exposures to BPA from food contact, medical devices, and other environmental sources. - Highlights: ? Maternal, placental, and fetal Phase II metabolism attenuate fetal exposure to BPA. ? Serum AUC for aglycone BPA in fetal monkeys is less than half of that in the dam. ? BPA profiles in monkey fetus rule out selective deconjugation and accumulation. ? BPA levels in monkey placenta are similar to other metabolically active tissues. ? Some published human cord blood data for BPA are inconsistent with these measurements.

  18. Differential modulation of dibenzo[def,p]chrysene transplacental carcinogenesis: Maternal diets rich in indole-3-carbinol versus sulforaphane

    SciTech Connect (OSTI)

    Shorey, Lyndsey E.; Madeen, Erin P.; Atwell, Lauren L.; Ho, Emily; Lhr, Christiane V.; Pereira, Clifford B.; Dashwood, Roderick H.; Williams, David E.


    Cruciferous vegetable components have been documented to exhibit anticancer properties. Targets of action span multiple mechanisms deregulated during cancer progression, ranging from altered carcinogen metabolism to the restoration of epigenetic machinery. Furthermore, the developing fetus is highly susceptible to changes in nutritional status and to environmental toxicants. Thus, we have exploited a mouse model of transplacental carcinogenesis to assess the impact of maternal dietary supplementation on cancer risk in offspring. In this study, transplacental and lactational exposure to a maternal dose of 15 mg/Kg B.W. of dibenzo[def,p]chrysene (DBC) resulted in significant morbidity of offspring due to an aggressive T-cell lymphoblastic lymphoma. As in previous studies, indole-3-carbinol (I3C, feed to the dam at 100, 500 or 1000 ppm), derived from cruciferous vegetables, dose-dependently reduced lung tumor multiplicity and also increased offspring survival. Brussels sprout and broccoli sprout powders, selected for their relative abundance of I3C and the bioactive component sulforaphane (SFN), respectively, surprisingly enhanced DBC-induced morbidity and tumorigenesis when incorporated into the maternal diet at 10% wt/wt. Purified SFN, incorporated in the maternal diet at 400 ppm, also decreased the latency of DBC-dependent morbidity. Interestingly, I3C abrogated the effect of SFN when the two purified compounds were administered in equimolar combination (500 ppm I3C and 600 ppm SFN). SFN metabolites measured in the plasma of neonates positively correlated with exposure levels via the maternal diet but not with offspring mortality. These findings provide justification for further study of the safety and bioactivity of cruciferous vegetable phytochemicals at supplemental concentrations during the perinatal period. - Highlights: Dietary supplementation may modulate cancer risk in a mouse model of lymphoma. Cruciferous vegetables may not contain sufficient I3C for

  19. Toxicogenomic profiling in maternal and fetal rodent brains following gestational exposure to chlorpyrifos

    SciTech Connect (OSTI)

    Moreira, Estefania G.; Yu Xiaozhong; Robinson, Joshua F.; Griffith, Willian; Hong, Sung Woo; Beyer, Richard P.; Bammler, Theo K.; Faustman, Elaine M.


    Considering the wide variety of effects that have been reported to occur in the developmental neurotoxicity of chlorpyrifos (CP) and the lack of consensus on their dependence of brain acetylcholinesterase (AChE) activity inhibition, we applied microarray technology to explore dose-dependent alterations in transcriptional response in the fetal and maternal C57BL/6 mouse brain after daily gestational exposure (days 6 to 17) to CP (2, 4, 10, 12 or 15 mg/kg, sc). We identified significantly altered genes across doses and assessed for overrepresentation of Gene Ontology (GO) biological processes and KEGG pathways. We further clustered genes based on their expression profiles across doses and repeated the GO/pathways analysis for each cluster. The dose-effect relationship of CP on gene expression, both at the gene and pathway levels was non-monotonic and not necessarily related to brain AChE inhibition. The largest impact was observed in the 10 mg/kg dose group which was also the LOAEL for brain AChE inhibition. In the maternal brain, lower doses (4 mg/kg) influenced GO categories and pathways such as cell adhesion, behavior, lipid metabolism, long-term potentiation, nervous system development, neurogenesis, synaptic transmission. In the fetal brain, lower doses (2 and/or 4 mg/kg) significantly altered cell division, translation, transmission of nerve impulse, chromatin modification, long-term potentiation. In addition, some genes involved in nervous system development and signaling were shown to be specifically influenced by these lower CP doses. Our approach was sensitive and reflected the diversity of responses known to be disrupted by CP and highlighted possible additional consequences of CP neurotoxicity, such as disturbance of the ubiquitin proteasome system.

  20. Maternal exposure to cadmium during gestation perturbs the vascular system of the adult rat offspring

    SciTech Connect (OSTI)

    Ronco, Ana Maria; Montenegro, Marcela; Castillo, Paula; Urrutia, Manuel; Saez, Daniel; Hirsch, Sandra; Zepeda, Ramiro; Llanos, Miguel N.


    Several cardiovascular diseases (CVD) observed in adulthood have been associated with environmental influences during fetal growth. Here, we show that maternal exposure to cadmium, a ubiquitously distributed heavy metal and main component of cigarette smoke is able to induce cardiovascular morpho-functional changes in the offspring at adult age. Heart morphology and vascular reactivity were evaluated in the adult offspring of rats exposed to 30 ppm of cadmium during pregnancy. Echocardiographic examination shows altered heart morphology characterized by a concentric left ventricular hypertrophy. Also, we observed a reduced endothelium-dependent reactivity in isolated aortic rings of adult offspring, while endothelium-independent reactivity remained unaltered. These effects were associated with an increase of hem-oxygenase 1 (HO-1) expression in the aortas of adult offspring. The expression of HO-1 was higher in females than males, a finding likely related to the sex-dependent expression of the vascular cell adhesion molecule 1 (VCAM-1), which was lower in the adult female. All these long-term consequences were observed along with normal birth weights and absence of detectable levels of cadmium in fetal and adult tissues of the offspring. In placental tissues however, cadmium levels were detected and correlated with increased NF-{kappa}B expression - a transcription factor sensitive to inflammation and oxidative stress - suggesting a placentary mechanism that affect genes related to the development of the cardiovascular system. Our results provide, for the first time, direct experimental evidence supporting that exposure to cadmium during pregnancy reprograms cardiovascular development of the offspring which in turn may conduce to a long term increased risk of CVD.

  1. Interactive effects of maternal and environmental exposure to coal combustion wastes decrease survival of larval southern toads (Bufo terrestris)

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Interactive effects of maternal and environmental exposure to coal combustion wastes decrease survival of larval southern toads (Bufo terrestris) Brian S. Metts a, * , Kurt A. Buhlmann a , David E. Scott a , Tracey D. Tuberville a , William A. Hopkins b a Savannah River Ecology Laboratory, Drawer E, Aiken, SC 29802, USA b Department of Fish and Wildlife Conservation, Virginia Tech, Blacksburg, VA 24061, USA a r t i c l e i n f o Article history: Received 16 August 2011 Received in revised form

  2. Meiotic interstrand DNA damage escapes paternal repair and causes chromosomal aberrations in the zygote by maternal misrepair


    Marchetti, Francesco; Bishop, Jack; Gingerich, John; Wyrobek, Andrew J.


    De novo point mutations and chromosomal structural aberrations (CSA) detected in offspring of unaffected parents show a preferential paternal origin with higher risk for older fathers. Studies in rodents suggest that heritable mutations transmitted from the father can arise from either paternal or maternal misrepair of damaged paternal DNA, and that the entire spermatogenic cycle can be at risk after mutagenic exposure. Understanding the susceptibility and mechanisms of transmission of paternal mutations is important in family planning after chemotherapy and donor selection for assisted reproduction. We report that treatment of male mice with melphalan (MLP), a bifunctional alkylating agent widelymore » used in chemotherapy, induces DNA lesions during male mouse meiosis that persist unrepaired as germ cells progress through DNA repair-competent phases of spermatogenic development. After fertilization, unrepaired sperm DNA lesions are mis-repaired into CSA by the egg's DNA repair machinery producing chromosomally abnormal offspring. In conclusion, these findings highlight the importance of both pre- and post-fertilization DNA repair in assuring the genomic integrity of the conceptus.« less

  3. Combined effects of perfluorooctane sulfonate (PFOS) and maternal restraint stress on hypothalamus adrenal axis (HPA) function in the offspring of mice

    SciTech Connect (OSTI)

    Ribes, Diana; Fuentes, Silvia; Torrente, Margarita; Colomina, M. Teresa [Department of Psychology and Research Center for Behavioral Assessment (CRAMC), 'Rovira i Virgili' University, Sescelades Campus, 43007 Tarragona, Catalonia (Spain); Laboratory of Toxicology and Environmental Health, School of Medicine, IISPV, 'Rovira i Virgili' University, Sant Llorenc 21, 43201 Reus, Catalonia (Spain); Domingo, Jose L., E-mail: [Laboratory of Toxicology and Environmental Health, School of Medicine, IISPV, 'Rovira i Virgili' University, Sant Llorenc 21, 43201 Reus, Catalonia (Spain)


    Although it is known that prenatal exposure to perfluorooctane sulfonate (PFOS) can cause developmental adverse effects in mammals, the disruptive effects of this compound on hormonal systems are still controversial. Information concerning the effects of PFOS on hypothalamus adrenal (HPA) axis response to stress and corticosterone levels is not currently available. On the other hand, it is well established that stress can enhance the developmental toxicity of some chemicals. In the present study, we assessed the combined effects of maternal restraint stress and PFOS on HPA axis function in the offspring of mice. Twenty plug-positive female mice were divided in two groups. Animals were given by gavage 0 and 6 mg PFOS/kg/day on gestation days 12-18. One half of the animals in each group were also subjected to restraint stress (30 min/session, 3 sessions/day) during the same period. Five plug-positive females were also included as non-manipulated controls. At 3 months of age, activity in an open-field and the stress response were evaluated in male and female mice by exposing them to 30 min of restraint stress. Male and female offspring were subsequently sacrificed and blood samples were collected to measure changes in corticosterone levels at four different moments related to stress exposure conditions: before stress exposure, immediately after 30 min of stress exposure, and recuperation levels at 60 and 90 min after stress exposure. Results indicate corticosterone levels were lower in mice prenatally exposed to restraint. In general terms, PFOS exposure decreased corticosterone levels, although this effect was only significant in females. The recuperation pattern of corticosterone was mainly affected by prenatal stress. Interactive effects between PFOS and maternal stress were sex dependent. The current results suggest that prenatal PFOS exposure induced long-lasting effects in mice.

  4. Influence of metal(loid) bioaccumulation and maternal transfer on embryo-larval development in fish exposed to a major coal ash spill


    Greeley, Jr., Mark Stephen; Adams, S. Marshall; Elmore, Logan R.; McCracken, Mary Kitty


    In December 2008, an earthen retaining wall at the Tennessee Valley Authority (TVA) Kingston Fossil Fuel Plant failed and released 4.1 million m3 of coal ash to rivers flowing into Watts Bar Reservoir in east Tennessee, United States (U.S.). As part of a comprehensive effort to evaluate the risks to aquatic resources from this spill – the largest in U.S. history – we compared bioaccumulation and maternal transfer of selenium (Se), arsenic (As), and mercury (Hg) in adult redear sunfish (Lepomis macrolophus), collected two years after the spill from both coal-ash exposed and non-exposed areas of the Emory and Clinchmore » Rivers, with the success of embryo-larval development in their offspring. Whole body and ovary concentrations of Se in female sunfish at three study sites downstream of the spill were significantly elevated (site means = 4.9–5.3 and 6.7–9.0 mg/kg d.w. whole body and ovary concentrations, respectively) compared with concentrations in fish from reference sites upstream of the spill site (2.2–3.2 mg/kg d.w. for whole bodies and 3.6–4.8 mg/kg d.w. for ovaries). However, Se concentrations in coal ash-exposed areas remain below proposed U.S. Environmental Protection Agency (USEPA) criteria for the protection of aquatic life. Site-to-site variation in fish concentrations of As and Hg were not well-correlated with ash-exposure, reflecting the multiple sources of these metal(loid)s in the affected watersheds. In 7-day laboratory tests of embryos and larvae derived from in vitro crosses of eggs and sperm from these field-collected sunfish, fertilization success, hatching success, embryo-larval survival, and incidences of developmental abnormalities did not differ significantly between ash-exposed and non-exposed fish. Furthermore, these developmental endpoints were not correlated with whole body or ovary concentrations of Se, As, or Hg in the maternal fish, or with fish size, ovary weight, or gonadal-somatic indices. Furthermore, results from

  5. Advancements in Orthopedic Intervention: Retrograde Drilling and Bone Grafting of Osteochondral Lesions of the Knee Using Magnetic Resonance Imaging Guidance

    SciTech Connect (OSTI)

    Seebauer, Christian J.; Bail, Hermann J.; Rump, Jens C. Walter, Thula Teichgraeber, Ulf K. M.


    Computer-assisted surgery is currently a novel challenge for surgeons and interventional radiologists. Magnetic resonance imaging (MRI)-guided procedures are still evolving. In this experimental study, we describe and assess an innovative passive-navigation method for MRI-guided treatment of osteochondritis dissecans of the knee. A navigation principle using a passive-navigation device was evaluated in six cadaveric knee joint specimens for potential applicability in retrograde drilling and bone grafting of osteochondral lesions using MRI guidance. Feasibility and accuracy were evaluated in an open MRI scanner (1.0 T Philips Panorama HFO MRI System). Interactive MRI navigation allowed precise drilling and bone grafting of osteochondral lesions of the knee. All lesions were hit with an accuracy of 1.86 mm in the coronal plane and 1.4 mm the sagittal plane. Targeting of all lesions was possible with a single drilling. MRI allowed excellent assessment of correct positioning of the cancellous bone cylinder during bone grafting. The navigation device and anatomic structures could be clearly identified and distinguished throughout the entire drilling procedure. MRI-assisted navigation method using a passive navigation device is feasible for the treatment of osteochondral lesions of the knee under MRI guidance and allows precise and safe drilling without exposure to ionizing radiation. This method may be a viable alternative to other navigation principles, especially for pediatric and adolescent patients. This MRI-navigated method is also potentially applicable in many other MRI-guided interventions.

  6. SU-D-204-06: Dose and Image Quality Evaluation of a Low-Dose Slot-Scanning X-Ray System for Pediatric Orthopedic Studies

    SciTech Connect (OSTI)

    Liu, Z; Hoerner, M; Lamoureux, R; Rill, L; Arreola, M


    Purpose: Children in early teens with scoliosis require repeated radiographic exams over a number of years. The EOS (EOS imaging S.A., Paris, France) is a novel low-dose slot-scanning digital radiographic system designed to produce full-spine images of a free-standing patient. The radiation dose and image quality characteristics of the EOS were evaluated relative to those of a Computed Radiography (CR) system for scoliosis imaging. Methods: For dose evaluation, a full-torso anthropomorphic phantom was scanned five times using the default standard clinical protocols for both the EOS and a CR system, which include both posteroanterior and lateral full-spine views. Optically stimulated luminescent dosimeters (OSLDs), also known as nanoDots™ (Landauer, Inc., Glenwood, IL), were placed on the phantom’s surface to measure entrance skin dose. To assess image quality, MTF curves were generated from sampling the noise levels within the high-contrast regions of a line-pair phantom. Vertical and horizontal distortions were measured for the square line-pair phantom with the EOS system to evaluate the effects of geometric magnification and misalignment with the indicated imaging plane. Results: The entrance skin dose was measured to be 0.4 to 1.1 mGy for the EOS, and 0.7 to 3.6 mGy for the CR study. MTF comparison shows that CR greatly outperforms the EOS, despite both systems having a limiting resolution at 1.8 line-pairs per mm. Vertical distortion was unaffected by phantom positioning, because of the EOS slot-scanning geometry. Horizontal distortion increased linearly with miscentering distance. Conclusion: The EOS system resulted in approximately 70% lower radiation dose than CR for full-spine images. Image quality was found to be inferior to CR. Further investigation is required to see if EOS system is an acceptable modality for performing clinically diagnostic scoliosis examinations.

  7. Tat-CBR1 inhibits inflammatory responses through the suppressions of NF-κB and MAPK activation in macrophages and TPA-induced ear edema in mice

    SciTech Connect (OSTI)

    Kim, Young Nam; Kim, Dae Won; Jo, Hyo Sang; Shin, Min Jea; Ahn, Eun Hee; Ryu, Eun Ji; Yong, Ji In; Cha, Hyun Ju; Kim, Sang Jin; Yeo, Hyeon Ji; Youn, Jong Kyu; Hwang, Jae Hyeok; Jeong, Ji-Heon; Kim, Duk-Soo; Cho, Sung-Woo; Park, Jinseu; Eum, Won Sik; Choi, Soo Young


    Human carbonyl reductase 1 (CBR1) plays a crucial role in cell survival and protects against oxidative stress response. However, its anti-inflammatory effects are not yet clearly understood. In this study, we examined whether CBR1 protects against inflammatory responses in macrophages and mice using a Tat-CBR1 protein which is able to penetrate into cells. The results revealed that purified Tat-CBR1 protein efficiently transduced into Raw 264.7 cells and inhibited lipopolysaccharide (LPS)-induced cyclooxygenase-2 (COX-2), nitric oxide (NO) and prostaglandin E{sub 2} (PGE{sub 2}) expression levels. In addition, Tat-CBR1 protein leads to decreased pro-inflammatory cytokine expression through suppression of nuclear transcription factor-kappaB (NF-κB) and mitogen activated protein kinase (MAPK) activation. Furthermore, Tat-CBR1 protein inhibited inflammatory responses in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation when applied topically. These findings indicate that Tat-CBR1 protein has anti-inflammatory properties in vitro and in vivo through inhibition of NF-κB and MAPK activation, suggesting that Tat-CBR1 protein may have potential as a therapeutic agent against inflammatory diseases. - Highlights: • Transduced Tat-CBR1 reduces LPS-induced inflammatory mediators and cytokines. • Tat-CBR1 inhibits MAPK and NF-κB activation. • Tat-CBR1 ameliorates inflammation response in vitro and in vivo. • Tat-CBR1 may be useful as potential therapeutic agent for inflammation.

  8. Enter filename

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    sources, prenatal radiation exposure, maternal or child medications, pesticides, second-hand cigarette smoke, factors associated with maternal pregnancy history (maternal age...

  9. Improved medical implants comes from nanostructuring

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    However, cobalt-chromium superalloys, stainless steels, and titanium alloys-the most commonly used materials in today's orthopedic devices- have the potential to present serious ...

  10. 1

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    medical implants comes from nanostructuring April 3, 2012 Quality of treatment for those with medical implants improves as science and medicine unite once more Metals have provided the strength, durability, and other characteristics that have been needed in bone implants since the inception of orthopedics. However, cobalt-chromium superalloys, stainless steels, and titanium alloys-the most commonly used materials in today's orthopedic devices- have the potential to present serious side effects.

  11. A role for glutathione, independent of oxidative stress, in the developmental toxicity of methanol

    SciTech Connect (OSTI)

    Siu, Michelle T.; Shapiro, Aaron M.; Wiley, Michael J.; Wells, Peter G.


    Oxidative stress and reactive oxygen species (ROS) have been implicated in the teratogenicity of methanol (MeOH) in rodents, both in vivo and in embryo culture. We explored the ROS hypothesis further in vivo in pregnant C57BL/6J mice. Following maternal treatment with a teratogenic dose of MeOH, 4 g/kg via intraperitoneal (ip) injection on gestational day (GD) 12, there was no increase 6 h later in embryonic ROS formation, measured by 2′,7′-dichlorodihydrofluorescin diacetate (DCFH-DA) fluorescence, despite an increase observed with the positive control ethanol (EtOH), nor was there an increase in embryonic oxidatively damaged DNA, quantified as 8-oxo-2′-deoxyguanosine (8-oxodG) formation. MeOH teratogenicity (primarily ophthalmic anomalies, cleft palate) also was not altered by pre- and post-treatment with varying doses of the free radical spin trapping agent alpha-phenyl-N-tert-butylnitrone (PBN). In contrast, pretreatment with L-buthionine-(S,R)-sulfoximine (BSO), an inhibitor of glutathione (GSH) synthesis, depleted maternal hepatic and embryonic GSH, and enhanced some new anomalies (micrognathia, agnathia, short snout, fused digits, cleft lip, low set ears), but not the most common teratogenic effects of MeOH (ophthalmic anomalies, cleft palate) in this strain. These results suggest that ROS did not contribute to the teratogenic effects of MeOH in this in vivo mouse model, in contrast to results in embryo culture from our laboratory, and that the protective effect of GSH in this model may arise from its role as a cofactor for formaldehyde dehydrogenase in the detoxification of formaldehyde. - Highlights: • In vivo, a free radical scavenger did not block methanol (MeOH) teratogenesis. • MeOH did not increase embryonic reactive oxygen species formation or DNA oxidation. • MeOH teratogenesis was enhanced by glutathione (GSH) depletion. • GSH may protect as the cofactor for formaldehyde dehydrogenase (ADH3). • Formaldehyde may be a ROS

  12. High-Efficiency Commercial Cold Climate Heat Pump

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    ... are met during development to accelerate future transition. ... This page contains no technical data subject to the EAR or ... analysis 10 Project Integration and Collaboration ...

  13. High-Efficiency Commercial Cold Climate Heat Pump

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    ... Project is in early stages of development Project Integration and Collaboration 11 This page contains no technical data subject to the EAR or the ITAR. Project Start Compressor ...

  14. Micro-Earthquake At Coso Geothermal Area (1987) | Open Energy...

    Open Energy Information (Open El) [EERE & EIA]

    variations in the Coso region, California, derived from local earthquake travel times Additional References Retrieved from "http:en.openei.orgwindex.php?titleMicro-Ear...

  15. High-Efficiency Commercial Cold Climate Heat Pump

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    ... AwardsRecognition: Project is in early stages of development This page contains no technical data subject to the EAR or the ITAR. 7 Project Integration and Collaboration Project ...


    Energy Savers

    ... The full text of this decision can be found at http:energy.govsitesprodfiles201406f16PSH-14-0011.pdf. U.S. D E epartment of nergy O H A ffice of earings and ppeals 2014 A ...

  17. Dr. Chuck Ross gives final lecture of JLab Spring Science Series...

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    the Civil War. By David Macaulay, Daily Press March 28, 2008 NEWPORT NEWS - It won't be music to the ears of traditionalists, but Civil War Confederate Gen. Robert E. Lee might...

  18. CBEI - Fault Detection and Diagnostics (FDD) for Advanced RTUs

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    ... Expected cost of FDD Even for 6 ton RTU FDD can achieve 3 year payback based on cost estimates This page contains no technical data subject to the EAR or the ITAR. 9 LabView ...

  19. BlueChoice

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Surgeon (including maternity services that are not part of OBGyn global delivery fee and complications of pregnancy) 10% after deductible 5 10% after deductible 10% after...

  20. SREL Reprint #3208

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Previous maternal exposure to coal combustion wastes (CCW) reduced larval survival to metamorphosis up to 57% compared to larvae of unexposed females. Larvae reared on CCW ...

  1. Microsoft Word - Scott-CV2013Sept.docx

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    ... TD, Scott DE, and Hopkins WA. Maternal transfer of contaminants and reduced reproductive success of southern toads (Bufo Anaxyrus terrestris) exposed to coal combustion waste. ...

  2. SREL Reprint #3245

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Maternal transfer of contaminants and reduced reproductive success of southern toads (Bufo Anaxyrus terrestris) exposed to coal combustion waste Brian S. Metts1, Kurt A. ...

  3. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    rhic (2) catalysis (homogeneous), solar (photovoltaic), bio-inspired, charge ... In addition, maternal Cd exposure markedly repressed cell proliferation and increased ...

  4. Atomic radii for atoms with the 6s shell outermost: The effective atomic radius and the van der Waals radius from {sub 55}Cs to {sub 80}Hg

    SciTech Connect (OSTI)

    Tatewaki, Hiroshi; Hatano, Yasuyo; Noro, Takeshi; Yamamoto, Shigeyoshi


    We consider, for atoms from {sub 55}Cs to {sub 80}Hg, the effective atomic radius (r{sub ear}), which is defined as the distance from the nucleus at which the magnitude of the electric field is equal to that in He at one half of the equilibrium bond length of He{sub 2}. The values of r{sub ear} are about 50% larger than the mean radius of the outermost occupied orbital of 6s, . The value of r{sub ear} decreases from {sub 55}Cs to {sub 56}Ba and undergoes increases and decreases with rising nuclear charge from {sub 57}La to {sub 70}Y b. In fact r{sub ear} is understood as comprising two interlaced sequences; one consists of {sub 57}La, {sub 58}Ce, and {sub 64}Gd, which have electronic configuration (4f{sup n−1})(5d{sup 1})(6s{sup 2}), and the remaining atoms have configuration (4f{sup n})(6s{sup 2}). The sphere defined by r{sub ear} contains 85%–90% of the 6s electrons. From {sub 71}Lu to {sub 80}Hg the radius r{sub ear} also involves two sequences, corresponding to the two configurations 5d{sup n+1}6s{sup 1} and 5d{sup n}6s{sup 2}. The radius r{sub ear} according to the present methodology is considerably larger than r{sub vdW} obtained by other investigators, some of who have found values of r{sub vdW} close to .

  5. High-resolution radio observations of W50, the remnant associated with SS 433

    SciTech Connect (OSTI)

    Elston, R.; Baum, S.


    The NRAO VLA has been used to observe the radio source W50 with 30 arcsec and 1 arcmin resolutions at 20 cm. It is found that the overall structure of W50 is well delineated by filamentary structure. W50 possesses a nearly circular shell reminiscent of an evolved supernova remnant such as CTB1 and the Cygnus Loop. The most unusual features of W50 are the edge-brightened radio ears which extend beyond the shell. These ears appear morphologically distinct from the shell and have minimum energy pressures three times greater than are present in the shell. Thus, while no direct connection is observed between SS433 and the ears, it is suggested that the ears are the result of the interaction of the ram pressure of the collimated jets of SS433 with a supernova remnant shell. At 30 arcsec resolution the western ear of W50 is depolarized, suggesting that W50 and S74 are at a common distance of about 3 kpc. 27 references.

  6. Uranium (VI)Bis(imido) chalcogenate complexes:synthesis and density functional theory analysis

    SciTech Connect (OSTI)

    Spencer, Liam P; Batista, Enrique R; Boncella, James M; Yang, Ping; Scott, Brian L


    Bis(imido) uranium(VI) trans- and cis-dichalcogenate complexes with the general formula U(NtBu)2(EAr)2(OPPh3)2 (EAr = O-2-tBuC6H4, SPh, SePh, TePh) and U(NtBu)2(EAr)2(R2bpy) (EAr = SPh, SePh, TePh) (R2bpy = 4,4'-disubstituted-2,2'-bipyridyl, R = Me, tBu) have been prepared. This family of complexes includes the first reported monodentate selenolate and tellurolate complexes of uranium(VI). Density functional theory calculations show that covalent interactions in the U-E bond increase in the trans-dichalcogenate series U(NtBu)2(EAr)2(OPPh3)2 as the size of the chalcogenate donor increases and that both 5f and 6d orbital participation is important in the M-E bonds of U-S, U-Se, and U-Te complexes.

  7. Newark Neighbors Saving Energy | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Newark Neighbors Saving Energy Newark Neighbors Saving Energy April 2, 2010 - 2:32pm Addthis Joshua DeLung Rosa and Francisco Sanchez are ecstatic about the weatherization work done to their Newark, N.J., home. When Francisco explains the changes they have seen, his smile beams from ear to ear. "They replaced my boiler, put in some new CFL light bulbs and gave me some carbon monoxide detectors," he says. "I was happy when I got approved after filling out the application for


    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    FY 2008 Annual Report O H A ffice of earings ppeals & O H A ffice of earin s ppeals & g Doe/hg-0020 U.S. DEPARTMENT OF ENERGY U.S. DEPARTMENT OF ENERGY Printed with soy ink on recycled paper. Office of hearings and appeals Organization chart On September 4, 2008, OHA was honored to host a visit by Secretary of Energy Samuel W. Bodman U.S. D E O H A epartment of nergy ffice of earings and ppeals 2008 a R nnual eport 3 Director (HG-1) Deputy Director (HG-2) Personnel security and appeals

  9. sloan_qer_cheyenne_statement

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    E nergy R eview H earing o n E nergy I nfrastructure August 2 1, 2 014 Cheyenne, W Y Rep. ... stakeholders t o d raft l anguage w hich i s t hen v etted t hrough i nterested p arties. ...

  10. Microsoft Word - DoE-WorkforceIssues_PetroleumInfrastructure...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Petroleum T ransmission & D istribution W orkforce i n L ouisiana Stephen R . B arnes, P ... Five y ears a go, o ur g roup a t L SU b egan w orking w ith t he L ouisiana W orkforce C ...

  11. Microsoft Word - 4.11.2013 ANGA QER Written Statement.docx

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    S o w e a ppreciate t he emphasis o n t his s ubject d uring t he f irst y ear o f t he q ... M ore t han o ther f uels, w e a lso p rovide consistent, f lexible p ower t hat h elps m ...

  12. Audiometry (hearing test) [DOE]

    The audiogram is an evaluation of how well an individual can hear. Sounds are presented to the individual through earphones during the test. These sounds are presented at different levels of frequency and intensity. The human ear responds to the frequency or pitch of a sound and the intensity or loudness of the sound.

  13. Microsoft Word - Tri-State Case Study.docx (indexed) [DOE]

    hat primarily s erves m ore t han 1 2,000 r ural c ustomers, m any o f w hom h ave l ow---incomes l iving a t o r n ear poverty l evel a cross a m ulti---state r egion ( see m ap). ...

  14. New Report: Early Lessons Learned in Bringing SSL to Market [DOE]

    The U.S. Department of Energy (DOE) has published a new report that documents early challenges and lessons learned in the development of the SSL market. Entitled "Solid-State Lighting: Early Lessons Learned on the Way to Market," it summarizes ear

  15. Y

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Sequoia 4 WD SUV 5.7L V8 Auto Tier I I B in 5 141317 10913 NA 2016 FFV Toyota Tundra 2WD4WD Pickup 5.7L V8 Auto Tier I I B in 5 151318 11913 NA Model Y ear 2 016: A...

  16. WIPP Update 4_02_14

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    T hey d id n ot d etect a ny airborne c ontamination. T he s econd t eam entered t he m ... a ir m onitor n ear t he S alt S haft Station to p rovide a dditional m onitoring c ...

  17. CX-006244: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    Craig-Bears Ears-Hayden Substations Fiber Optic and Pole Installation, Moffat and Routt Counties, ColoradoCX(s) Applied: B4.6, B4.7Date: 05/17/2011Location(s): Moffat, ColoradoOffice(s): Western Area Power Administration-Rocky Mountain Region

  18. United States

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    ... Ellis (1993) collected fecal pellets from the roost of a maternity colony in North ... In summer, little brown bats roost under rocks, in piles of wood, and in trees (Fenton and ...

  19. Organ-on-a-chip | The Ames Laboratory

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    a "placenta-on-a-chip" in order to mimic the nutrientwaste transfer between maternal blood and fetal blood that occurs in the cotyledon section of the placenta, and to test and...

  20. National Strategy for the Arctic Region (NSAR) - 10-Year Renewable Energy Plan

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    NSAR - T en Y ear R enewable E nergy P lan - A pril 2 015 2 National S trategy f or t he A rctic R egion ( NSAR) Ten Y ear R enewable E nergy P lan Background The N ational S trategy f or t he A rctic R egion ( NSAR) o utlines t he U nited S tates G overnment's strategic p riorities f or t he A rctic r egion. T hese p riorities a re i ntended t o p osition t he U nited S tates to r espond e ffectively t o e merging o pportunities - w hile s imultaneously p ursuing e fforts t o protect a nd c

  1. nersc-for-hep-theory.pptx

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Ph.D.! NERSC User Services High Performance Computing and Big Data at NERSC --- 1 --- April 3 , 2 013 Outline * What i s N ERSC? * Who r uns a t N ERSC? * Can y ou u se/do y ou n eed H igh P erformance CompuDng a nd D ata S ervices? --- 2 --- What is NERSC? --- 3 --- NERSC is the High Performance Production Computing Center for the DOE Office of Science 4 NERSC's f ocus i s o n e nabling s cienDfic producDvity * 1,500 r efereed p ublica1ons p er y ear * ~10 m ajor j ournal c overs p er y ear *

  2. Using aural (audible) information generated directly from conventional NDE technology signals to aid the inspector in defect detection and discrimination

    SciTech Connect (OSTI)

    Holt, A.E.; Light, G.M.; Polk, K.D.; Clayton, W.T.


    During nondestructive evaluation (NDE) of a material, an NDE instrument typically is used to receive the inspection signals and display them for visual interpretation. Work has been underway to convert these signals to a range of aural (audible) signals with the intent to enhance the accuracy of evaluation through the use of two senses (ears and eyes) instead of one. This paper describes auralization of ultrasonic NDE testing signals to improve characterization and evaluation of materials.

  3. Aural perception of NDE signals

    SciTech Connect (OSTI)

    Light, G.M.; Holt, A.E.; Polk, K.D.; Godwin, J.G.; Clayton, W.T.


    During nondestructive evaluation (NDE) of a material, the inspection signals are received typically by an NDE instrument. These signals usually are displayed electronically for visual interpretation. Work has been done to convert these signals into aural (audible) signals with the intent to enhance the accuracy of evaluation through the use of two senses (ears and eyes) instead of one. This paper describes auralization of ultrasonic NDE testing signals to improve characterization and evaluation of materials.

  4. Issue

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    1 November 2006 A publication for all members of the NNSA/NSO family ear Admiral Gerald L. Talbot, Jr. has been named as the new manager of the National Nuclear Security Administration's Nevada Site Office. Upon his retirement, after serving 35-years in the United States Navy, Rear Admiral Talbot will assume his new position in January, 2007. He succeeds Kathy A. Carlson, who retired in May 2006. Rear Admiral Talbot currently serves as the Director, Military Personnel Plans and Policy Division.

  5. ITEP Webinar: About the Local Environmental Observer (LEO) Network [DOE]

    Attend this Institute for Tribal Environmental Professionals (ITEP) webinar and learn how the Local Environmental Observer (LEO) Network provides a model for engaging communities; connecting with technical experts and resources; and disseminating information about specific events and the impacts, needs, and responses. This webinar will introduce LEO as a resource comprised of local experts who collect observations about unusual environmental events and serve as the eyes, ears and voice of environmental change in their communities.

  6. 2001 ''You Have the Power'' campaign [Federal Energy Management Program]. Final technical report

    SciTech Connect (OSTI)


    The Tasks of 2001 ''You Have the Power'' campaign by the Federal Energy Management Program (FEMP) are: Task 1--Interagency Planning Meetings; Task 2--Ear Day Event; Task 3--Earth Day and Energy Awareness Month Activities; Task 4--Regional Target; Task 5--Outreach Tools and Campaign Products; Task 6--Private Sector Participation; Task 7--''You Have the Power'' on the FEMP Web Site; and Task 8--Effective Communications.

  7. Implications of a {open_quotes}cross-rolled{close_quotes} yield surface approximation on deep drawing

    SciTech Connect (OSTI)

    Logan, R.W.


    During deep-drawing, two issues manifest themselves that are due to normal and planar anisotropy in the sheet. These are a dependence of the Limiting Draw Ratio (LDR) on the average thinning ratio (R-value), and a dependence of ear formation and thinning around the circumference on the variation of R-value in the plane of the sheet. The quadratic (1948 Hill) yield surface has been applied to these issues and it has been demonstrated that there are numerous higher exponent yield criteria that may more closely duplicate experimental trends. These predict varying degrees of R-value dependencies of uniaxial yields and strength ratios in multiaxial loading paths. The result of this is that there are sometimes subtle and sometimes substantial differences in the predictions of the various yield surfaces on deep drawing regarding LDR and earing. Additional differences arise due to the way the shear term (45-degree yield) and the 0-degree vs. 90-degree strengths are treated in each criterion. These dependencies (in-plane strengths generated by the yield surfaces) are shown to affect the results of both LDR and earing during cupping. In particular, the 1979 Hosford and 1989 Barlat (Tricomponent) criteria, although identical for the normal anisotropy (planar isotropy) case, are strikingly different for cases where there are differences at 45 degrees and 90 degrees from the rolling direction.

  8. Improving the osteointegration of Ti6Al4V by zeolite MFI coating

    SciTech Connect (OSTI)

    Li, Yong; Jiao, Yilai; Li, Xiaokang; Guo, Zheng


    Osteointegration is crucial for success in orthopedic implantation. In recent decades, there have been numerous studies aiming to modify titanium alloys, which are the most widely used materials in orthopedics. Zeolites are solid aluminosilicates whose application in the biomedical field has recently been explored. To this end, MFI zeolites have been developed as titanium alloy coatings and tested in vitro. Nevertheless, the effect of the MFI coating of biomaterials in vivo has not yet been addressed. The aim of the present work is to evaluate the effects of MFI-coated Ti6Al4V implants in vitro and in vivo. After surface modification, the surface was investigated using field emission scanning electron microscopy (FE-SEM) and energy dispersive spectroscopy (EDS). No difference was observed regarding the proliferation of MC3T3-E1 cells on the Ti6Al4V (Ti) and MFI-coated Ti6Al4V (M−Ti) (p > 0.05). However, the attachment of MC3T3-E1 cells was found to be better in the M−Ti group. Additionally, ALP staining and activity assays and quantitative real-time RT-PCR indicated that MC3T3-E1 cells grown on the M−Ti displayed high levels of osteogenic differentiation markers. Moreover, Van-Gieson staining of histological sections demonstrated that the MFI coating on Ti6Al4V scaffolds significantly enhanced osteointegration and promoted bone regeneration after implantation in rabbit femoral condylar defects at 4 and 12 weeks. Therefore, this study provides a method for modifying Ti6Al4V to achieve improved osteointegration and osteogenesis. - Highlights: • Osteointegration is a crucial factor for orthopedic implants. • We coated MFI zeolite on Ti6Al4V substrates and investigated the effects in vitro and in vivo. • The MFI coating displayed good biocompatibility and promoted osteogenic differentiation in vitro. • The MFI coating promoted osteointegration and osteogenesis peri-implant in vivo.

  9. SREL Reprint #3335

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    5 Consequences of maternal effects on offspring size, growth and survival in the desert tortoise M. G. Nafus1,2, B. D. Todd1, K. A. Buhlmann3, and T. D. Tuberville3 1Department of Wildlife, Fish and Conservation Biology, University of California, Davis, CA, USA 2San Diego Zoological Global, San Diego Zoo Institute for Conservation Research, Escondido, CA, USA 3University of Georgia’s Savannah River Ecology Laboratory, Aiken, SC, USA Abstract: Maternal body size can have notable consequences on

  10. Method and product for phosphosilicate slurry for use in dentistry and related bone cements

    DOE Patents [OSTI]

    Wagh, Arun S.; Primus, Carolyn


    The present invention is directed to magnesium phosphate ceramics and their methods of manufacture. The composition of the invention is produced by combining a mixture of a substantially dry powder component with a liquid component. The substantially dry powder component comprises a sparsely soluble oxide powder, an alkali metal phosphate powder, a sparsely soluble silicate powder, with the balance of the substantially dry powder component comprising at least one powder selected from the group consisting of bioactive powders, biocompatible powders, fluorescent powders, fluoride releasing powders, and radiopaque powders. The liquid component comprises a pH modifying agent, a monovalent alkali metal phosphate in aqueous solution, the balance of the liquid component being water. The use of calcined magnesium oxide as the oxide powder and hydroxylapatite as the bioactive powder produces a self-setting ceramic that is particularly suited for use in dental and orthopedic applications.

  11. A cohort study of developmental polychlorinated biphenyl (PCB) exposure in relation to post-vaccination antibody response at 6-months of age

    SciTech Connect (OSTI)

    Jusko, Todd A.; De Roos, Anneclaire J.; Schwartz, Stephen M.; Program in Epidemiology, Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA ; Paige Lawrence, B.; Palkovicova, Lubica; Nemessanyi, Tomas; Drobna, Beata; Fabisikova, Anna; Kocan, Anton; Sonneborn, Dean; Jahnova, Eva; Kavanagh, Terrance J.; Trnovec, Tomas; Hertz-Picciotto, Irva


    Background: Extensive experimental data in animals indicate that exposure to polychlorinated biphenyls (PCBs) during pregnancy leads to changes in offspring immune function during the postnatal period. Whether developmental PCB exposure influences immunologic development in humans has received little study. Methods: The study population was 384 mother-infant pairs recruited from two districts of eastern Slovakia for whom prospectively collected maternal, cord, and 6-month infant blood specimens were available. Several PCB congeners were measured in maternal, cord, and 6-month infant sera by high-resolution gas chromatography with electron capture detection. Concentrations of IgG-specific anti-haemophilus influenzae type b, tetanus toxoid, and diphtheria toxoid were assayed in 6-month infant sera using ELISA methods. Multiple linear regression was used to estimate the relation between maternal, cord, and 6-month infant PCB concentrations and the antibody concentrations evaluated at 6-months of age. Results: Overall, there was little evidence of an association between infant antibody concentrations and PCB measures during the pre- and early postnatal period. In addition, our results did not show specificity in terms of associations limited to a particular developmental period (e.g. pre- vs. postnatal), a particular antibody, or a particular PCB congener. Conclusions: At the PCB concentrations measured in this cohort, which are high relative to most human populations today, we did not detect an association between maternal or early postnatal PCB exposure and specific antibody responses at 6-months of age.

  12. Identification and Protection of a Bat Colony in the 183-F Clearwell: Mitigation of Bat Habitat on the Hanford Site

    SciTech Connect (OSTI)

    K. A. Gano, J. G. Lucas, C. T. Lindsey


    An ecological investigation was conducted to evaluate mitigation options for demolition of a retired facility that contained a maternity roost of approximately 2,000 Myotis yumanensis bats. The recommendation from the study was to leave the non-contaminated structure intact and fence the area.

  13. Richard Gerber!

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Senior Science Advisor to the Director! NERSC Data-Driven Science at NERSC --- 1 --- August 8 , 2 013 New Community Focus on Data --- 2 --- DOE has extreme data needs --- 3 --- Light Sources * Many d etectors o n M oore's L aw c urve * Data v olumes r endering p revious o pera=onal m odels o bsolete High Energy Physics * LHC E xperiments p roduce a nd d istribute p etabytes o f d ata/year * Astro: p eak d ata r ates i ncrease 3 ---5x o ver 5 y ears, T B o f d ata p er n ight Genomics * Sequencer

  14. Gerhardt PDSF Intro.pptx

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    PDSF Consultant! ! April 29, 2014 PDSF Parallel Distributed Scientific Facility --- 2 --- * Rose f rom t he a shes o f t he S SC * Really c ame t o l ife i n 1 997 w hen N ERSC m oved t o L BNL STAR ALICE SNO AMANDA e895 KamLAND CDF Supernova Factory Many Years of Science --- 3 --- On t rack t o d eliver ~ 2500 CPU y ears i n 2 014 PDSF Today * Used by Nuclear Physics and High Energy Physics groups - SimulaMon a nd a nalysis - Data m irrors * Evergreen and condo * 2600 c ores w ith 2 0 - 6 0 G B

  15. Development of a phase-sensitive Fourier domain optical coherence tomography system to measure mouse organ of Corti vibrations in two cochlear turns

    SciTech Connect (OSTI)

    Ramamoorthy, Sripriya; Zhang, Yuan; Jacques, Steven; Petrie, Tracy; Wang, Ruikang; Nuttall, Alfred L.


    In this study, we have developed a phase-sensitive Fourier-domain optical coherence tomography system to simultaneously measure the in vivo inner ear vibrations in the hook area and second turn of the mouse cochlea. This technical development will enable measurement of intra-cochlear distortion products at ideal locations such as the distortion product generation site and reflection site. This information is necessary to un-mix the complex mixture of intra-cochlear waves comprising the DPOAE and thus leads to the non-invasive identification of the local region of cochlear damage.

  16. oganesian1_ab

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    SUPER H EAVY E LEMENTS Academician P rofessor Y uri O ganessian JJINR, D ubna, R ussian F ederation/Cyclotron I nstitute, T AMU Summary A s eries o f 5 l ectures i s d evoted t o o ne o f t he m ost f undamental p roblems o f m odern p hysics, n uclear chemistry and astrophysics - the problem of existence of hypothetical super heavy elements. Experimental s tudies o f t he p ast 1 5 y ears l ed t o a d iscovery o f s o---called " Island o f s tability" o f n eutron--- rich nuclei,

  17. 06-2015-2 |

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    The Infinite Possibilities of Partnership: CCSI, Collaboration, and Carbon Capture Elephant---Picture-One.jpg The whole is the sum of the parts. There is an old tale of six blind men asked to touch an elephant and identify what object they felt. The first touched the elephant's ear and concludes that he must be holding a fan. The next rested his hand on a tusk, and swore he was holding a spear. Each man, touching a different part of the beast, could only describe the small part that he felt;

  18. 06-DataManagement-Gerhardt.pdf

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    New User Training! July 15, 2014 Data Management at NERSC Where Do I Put My Data? --- 2 --- * Overview o f N ERSC fi le s ystems - Local v s. G lobal - Permanent v s. P urged * HPSS A rchive S ystem - What i s i t a nd h ow t o u se i t * Data S haring NERSC File Systems --- 3 --- The compute and storage systems 2014 Produc<on C lusters Carver, P DSF, J GI, MatComp, Planck /global/ scratch 4 PB /project 5 PB /home 250 TB 65 PB stored, 240 PB capacity, 4 0 y ears o f community d ata HPSS 16 x

  19. AmeriFlux US-Var Vaira Ranch- Ione

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Baldocchi, Dennis [University of California, Berkeley


    This is the AmeriFlux version of the carbon flux data for the site US-Var Vaira Ranch- Ione. Site Description - Located in the lower foothills of the Sierra Nevada Mountains on privately owned land, the Vaira Ranch site is classified as a grassland dominated by C3 annual grasses. Managed by local rancher, Fran Vaira, brush has been periodically removed for cattle grazing. Species include a variety of grasses and herbs, including purple false brome, smooth cat's ear, and rose clover. Growing season is confined to the wet season only, typically from October to early May.

  20. Descartes Labs Emerges With $5M to Improve Data for Farmers

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Descartes Labs Emerges With $5M to Improve Data for Farmers Descartes Labs Emerges With $5M to Improve Data for Farmers " real time, nobody knows how much corn is growing on the planet. One way our technology figures this out is by looking at light as it changes over the seasons. We're doing all this without seeing an ear of corn." November 17, 2015 Mark Johnson & Steven Brumby Mark Johnson, left, co-founder and CEO; and Steven Brumby, co-founder and CTO of Descartes Labs.

  1. Douglas Jacobsen! NERSC Bioinformatics Computing Consultant

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Working Remotely on NERSC Systems (genepool focus) --- 1 --- February 1 2, 2 013 Structure of the Genepool System --- 2 --- compute n odes gpint n odes high p riority & interac6ve nodes fpga web services database services login n odes filesystems ssh h=p:// User A ccess C ommand L ine S cheduler S ervice Types of Jobs on genepool * Batch - S cheduled ( compute n odes, f pga) - 8,320 c ores f or 7 2,953,280 c ompute h ours p er y ear i n g enepool - use

  2. Methods of and apparatus for levitating an eddy current probe

    DOE Patents [OSTI]

    Stone, William J.


    An eddy current probe is supported against the force of gravity with an air earing while being urged horizontally toward the specimen being examined by a spring and displaced horizontally against the force of the spring pneumatically. The pneumatic displacement is accomplished by flowing air between a plenum chamber fixed with respect to the probe and the surface of the specimen. In this way, the surface of the specimen can be examined without making mechanical contact therewith while precisely controlling the distance at which the probe stands-off from the surface of the specimen.

  3. DOE Challenge Home Verification

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    DOE Challenge Home Verification Projected Rating: Based on Plans - Field Confirmation Required. Energy Performance House Ty pe DOE Challenge Home Builder Partner ID# Single-family det ac hed 12345 Y ear built Square footage of Conditioned Space including Basement 2013 3968.0 Numbe r of Bedrooms Square footage of Conditioned Space without Basement 4 2368.0 Site addre ss (if no t available , list the site Lo t #) Registered Builder 555 Main St r eet Cold City Certified Rater MN, 20853 HERS Index

  4. DUF6 News | Department of Energy

    Energy Savers

    Chapter 25 June 2016 ______________________________________________________________________________ 1 Compliance with U.S. Export Control Laws, Regulations and Policies [References: FAR 17.6, DEAR 917.6, DEAR 925.71, AEA of 1954 as amended, DOE IG Report DOE/IG-0645, DOE IG Report INS-O-07-01, GAO Report GAO-11-354, EAA of 1979 as amended, EAR (15 CFR Parts 730-774), AECA (22 U.S.C. 2778), ITAR (22 CFR Parts 120-130), 10 CFR Part 810, 10 CFR Part 110, TWEA (50 U.S.C. App. 5(b) as amended), 31

  5. DRAFT

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Chapter 25 June 2016 ______________________________________________________________________________ 1 Compliance with U.S. Export Control Laws, Regulations and Policies [References: FAR 17.6, DEAR 917.6, DEAR 925.71, AEA of 1954 as amended, DOE IG Report DOE/IG-0645, DOE IG Report INS-O-07-01, GAO Report GAO-11-354, EAA of 1979 as amended, EAR (15 CFR Parts 730-774), AECA (22 U.S.C. 2778), ITAR (22 CFR Parts 120-130), 10 CFR Part 810, 10 CFR Part 110, TWEA (50 U.S.C. App. 5(b) as amended), 31

  6. Vertical distribution of structural components in corn stover

    SciTech Connect (OSTI)

    Johnson, Jane M. F.; Karlen, Douglas L.; Gresham, Garold L.; Cantrell, Keri B.; Archer, David W.; Wienhold, Brian J.; Varvel, Gary E.; Laird, David A.; Baker, John; Ochsner, Tyson E.; Novak, Jeff M.; Halvorson, Ardell D.; Arriaga, Francisco; Lightle, David T.; Hoover, Amber; Emerson, Rachel; Barbour, Nancy W.


    In the United States, corn (Zea mays L.) stover has been targeted for second generation fuel production and other bio-products. Our objective was to characterize sugar and structural composition as a function of vertical distribution of corn stover (leaves and stalk) that was sampled at physiological maturity and about three weeks later from multiple USA locations. A small subset of samples was assessed for thermochemical composition. Concentrations of lignin, glucan, and xylan were about 10% greater at grain harvest than at physiological maturity, but harvestable biomass was about 25% less due to stalk breakage. Gross heating density above the ear averaged 16.3 ± 0.40 MJ kg⁻¹, but with an alkalinity measure of 0.83 g MJ⁻¹, slagging is likely to occur during gasification. Assuming a stover harvest height of 10 cm, the estimated ethanol yield would be >2500 L ha⁻¹, but it would be only 1000 L ha⁻¹ if stover harvest was restricted to the material from above the primary ear. Vertical composition of corn stover is relatively uniform; thus, decision on cutting height may be driven by agronomic, economic and environmental considerations.

  7. Archaeofaunal insights on pinniped-human interactions in the northeastern Pacific

    SciTech Connect (OSTI)

    Gifford-Gonzales, D; Newsome, S; Koch, P; Guilderson, T; Snodgrass, J; Burton, R


    Human exploitation of pinnipeds has considerable antiquity but shows increasing impacts on population numbers in the Holocene. Pinnipeds are a rich source of fat as well as protein. A few well-documented cases of regional extirpation of seals and sea lions by non-industrial peoples exist. The northeastern Pacific region, from southern California to Alaska, has yielded archaeological evidence for distributions and abundances of eared seals that differs markedly from historically documented biogeography. This is especially true of the northern fur seal (Callorhinus ursinus), among the most common pinnipeds in many archaeological sites from the Santa Barbara Channel area through to Kodiak Islands. This chapter reviews contemporary eared seal biogeography, evidence for the earlier timing and extent, of occurrence of northern fur seals along the northeastern Pacific coast, zooarchaeological and isotopic evidence for their foraging and probable maintenance of rookeries in lower latitudes, and for their disappearance from the southernmost part of their ancient distribution well before European contact. It also reviews ongoing debates over the behavioral ecology of ancient fur seals and over humans role in contributing to their disappearance.

  8. Bat habitat research. Final technical report

    SciTech Connect (OSTI)

    Keller, B.L.; Bosworth, W.R.; Doering, R.W.


    This progress report describes activities over the current reporting period to characterize the habitats of bats on the INEL. Research tasks are entitled Monitoring bat habitation of caves on the INEL to determine species present, numbers, and seasons of use; Monitor bat use of man-made ponds at the INEL to determine species present and rates of use of these waters; If the Big Lost River is flowing on the INEL and/or if the Big Lost River sinks contain water, determine species present, numbers and seasons of use; Determine the habitat requirement of Townsend`s big-eared bats, including the microclimate of caves containing Townsend`s big-eared bats as compared to other caves that do not contain bats; Determine and describe an economical and efficient bat census technique to be used periodically by INEL scientists to determine the status of bats on the INEL; and Provide a suggestive management and protective plan for bat species on the INEL that might, in the future, be added to the endangered and sensitive list;

  9. Vertical distribution of structural components in corn stover

    SciTech Connect (OSTI)

    Jane M. F. Johnson; Douglas L. Karlen; Garold L. Gresham; Keri B. Cantrell; David W. Archer; Brian J. Wienhold; Gary E. Varvel; David A. Laird; John Baker; Tyson E. Ochsner; Jeff M. Novak; Ardell D. Halvorson; Francisco Arriaga; David T. Lightle; Amber Hoover; Rachel Emerson; Nancy W. Barbour


    In the United States, corn (Zea mays L.) stover has been targeted for second generation fuel production and other bio-products. Our objective was to characterize sugar and structural composition as a function of vertical distribution of corn stover (leaves and stalk) that was sampled at physiological maturity and about three weeks later from multiple USA locations. A small subset of samples was assessed for thermochemical composition. Concentrations of lignin, glucan, and xylan were about 10% greater at grain harvest than at physiological maturity, but harvestable biomass was about 25% less due to stalk breakage. Gross heating density above the ear averaged 16.3 0.40 MJ kg?, but with an alkalinity measure of 0.83 g MJ?, slagging is likely to occur during gasification. Assuming a stover harvest height of 10 cm, the estimated ethanol yield would be >2500 L ha?, but it would be only 1000 L ha? if stover harvest was restricted to the material from above the primary ear. Vertical composition of corn stover is relatively uniform; thus, decision on cutting height may be driven by agronomic, economic and environmental considerations.

  10. Anti-inflammatory activity of methyl palmitate and ethyl palmitate in different experimental rat models

    SciTech Connect (OSTI)

    Saeed, Noha M.; El-Demerdash, Ebtehal; Abdel-Rahman, Hanaa M.; Algandaby, Mardi M.; Al-Abbasi, Fahad A.; Abdel-Naim, Ashraf B.


    Methyl palmitate (MP) and ethyl palmitate (EP) are naturally occurring fatty acid esters reported as inflammatory cell inhibitors. In the current study, the potential anti-inflammatory activity of MP and EP was evaluated in different experimental rat models. Results showed that MP and EP caused reduction of carrageenan-induced rat paw edema in addition to diminishing prostaglandin E2 (PGE2) level in the inflammatory exudates. In lipopolysaccharide (LPS)-induced endotoxemia in rats, MP and EP reduced plasma levels of tumor necrosis factor-? (TNF-?) and interleukin-6 (IL-6). MP and EP decreased NF-?B expression in liver and lung tissues and ameliorated histopathological changes caused by LPS. Topical application of MP and EP reduced ear edema induced by croton oil in rats. In the same animal model, MP and EP reduced neutrophil infiltration, as indicated by decreased myeloperoxidase (MPO) activity. In conclusion, this study demonstrates the effectiveness of MP and EP in combating inflammation in several experimental models. -- Highlights: ? Efficacy of MP and EP in combating inflammation was displayed in several models. ? MP and EP reduced carrageenan-induced rat paw edema and prostaglandin E2 level. ? MP and EP decreased TNF-? and IL-6 levels in experimental endotoxemia. ? MP and EP reduced NF-?B expression and histological changes in rat liver and lung. ? MP and EP reduced croton oil-induced ear edema and neutrophil infiltration.

  11. Tuberville Lab Personnel | Savannah River Ecology Laboratory

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Lab Personnel RESEARCH STAFF Kimberly wih pine snake Kimberly Price is a new research technician in the Tuberville lab. Kimberly graduated in 2015 with her B.S. in Ecology from Augusta University in Augusta, Georgia. While a student working with Dr. Brandon R. Cromer, she focused her undergraduate research on contamination and maternal transfer of mercury in southeastern aquatic turtles using the yellow-bellied slider (Trachemys scripta scripta) and the eastern river cooter (Pseudemys concinna

  12. DNA repair efficiency in germ cells and early mouse embryos and consequences for radiation-induced transgenerational genomic damage

    SciTech Connect (OSTI)

    Marchetti, Francesco; Wyrobek, Andrew J.


    Exposure to ionizing radiation and other environmental agents can affect the genomic integrity of germ cells and induce adverse health effects in the progeny. Efficient DNA repair during gametogenesis and the early embryonic cycles after fertilization is critical for preventing transmission of DNA damage to the progeny and relies on maternal factors stored in the egg before fertilization. The ability of the maternal repair machinery to repair DNA damage in both parental genomes in the fertilizing egg is especially crucial for the fertilizing male genome that has not experienced a DNA repair-competent cellular environment for several weeks prior to fertilization. During the DNA repair-deficient period of spermatogenesis, DNA lesions may accumulate in sperm and be carried into the egg where, if not properly repaired, could result in the formation of heritable chromosomal aberrations or mutations and associated birth defects. Studies with female mice deficient in specific DNA repair genes have shown that: (i) cell cycle checkpoints are activated in the fertilized egg by DNA damage carried by the sperm; and (ii) the maternal genotype plays a major role in determining the efficiency of repairing genomic lesions in the fertilizing sperm and directly affect the risk for abnormal reproductive outcomes. There is also growing evidence that implicates DNA damage carried by the fertilizing gamete as a mediator of postfertilization processes that contribute to genomic instability in subsequent generations. Transgenerational genomic instability most likely involves epigenetic mechanisms or error-prone DNA repair processes in the early embryo. Maternal and embryonic DNA repair processes during the early phases of mammalian embryonic development can have far reaching consequences for the genomic integrity and health of subsequent generations.


    SciTech Connect (OSTI)

    Brigmon, R.


    Nanostructured materials may play a significant role in controlled release of pharmacologic agents for treatment of cancer. Many nanoporous polymer materials are inadequate for use in drug delivery. Nanoporous alumina provides several advantages over other materials for use in controlled drug delivery and other medical applications. Atomic layer deposition was used to coat all the surfaces of the nanoporous alumina membrane in order to reduce the pore size in a controlled manner. Both the 20 nm and 100 nm titanium oxide-coated nanoporous alumina membranes did not exhibit statistically lower viability compared to the uncoated nanoporous alumina membrane control materials. In addition, 20 nm pore size titanium oxide-coated nanoporous alumina membranes exposed to ultraviolet light demonstrated activity against Escherichia coli and Staphylococcus aureus bacteria. Nanostructured materials prepared using atomic layer deposition may be useful for delivering a pharmacologic agent at a precise rate to a specific location in the body. These materials may serve as the basis for 'smart' drug delivery devices, orthopedic implants, or self-sterilizing medical devices.

  14. Y

    Alternative Fuels and Advanced Vehicles Data Center

    Model Y ear 2 015: A lternative F uel a nd A dvanced T echnology V ehicles 1 (Updated 1 1/24/2014) 1 Source:http/ V ehicle S earch MY Fuel/Powertrain T ype Make Model Vehicle T ype Engine S ize/Cylinders Transmission Emissions C lass 2 Fuel E conomy Gasoline 3,4 City/Hwy Fuel E conomy A lt Fuel 3,4 City/Hwy All E lectric Range ( mi) 2015 B20 Chevrolet Cruze Sedan 2.0L I 4 diesel Auto Tier I I B in 5 LEV I II L EV160 27/46 N/A N/A 2015 B20 Chevrolet

  15. Richard Gerber! NERSC Senior Science Advisor! User Services Group Lead

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    User Services Group Lead NUG Webinar January 2014 --- 1 --- January 9 , 2 014 Agenda * Edison C onfigura6on f or A Y 2 014 * Alloca6on Y ear R ollover I ssues * Project D irectories p er P roject * NUG 2 014 U ser G roup M ee6ng * Automa6c I /O P rofiling f or Y our C ode * Give / T ake U 6lity f or S haring F iles * NUG Q ueue C ommiRee U pdate * User Survey Needs Your Input * Open D iscussion --- 2 --- Edison Update --- 3 --- Jeff B roughton NERSC D eputy f or O pera6ons Systems D epartment H

  16. Audible radiation monitor

    DOE Patents [OSTI]

    Odell, Daniel M. C.


    A method and apparatus for monitoring ionizing radiation comprising radiation detectors in electrical connection with an isotopic analyzer and a device for producing chords to which each isotope is mapped so that the device produces a unique chord for each isotope. Preferably the chords are pleasing to the ear, except for chords representing unexpected isotopes, and are louder or softer depending on the level of radioactivity produced by each isotope, and musical instrument voices may be simulated in producing the chords as an aid to distinguishing similar-sounding chords. Because of the representation by chords, information regarding the level and composition of the radiation in an area can be conveyed to workers in that area more effectively and yet without distracting them.

  17. WIPP Update 4_01_14

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    , 2 014 Delay i n D elivery o f P rotective E quipment P ostpones P ersonnel R e---Entry Today's s cheduled p ersonnel r e---entry t o t he W IPP u nderground was p ostponed d ue t o a delivery d elay of the lapel m onitors w orkers w ould w ear o n t heir o uter c lothing t o d etect r adiation. Safety requirements for r e---entry w ork r equire t his t ype o f p ersonal p rotective e quipment a nd e ntry w ill b e postponed u ntil t hey a rrive. Community M eetings S cheduled April 3 --- C

  18. WIPP Update 5_21_14

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    2 1, 2 014 WIPP R eviewing N MED A dministrative O rder Yesterday, t he N ew M exico E nvironment D epartment issued an A dministrative O rder r equiring t he Waste I solation P ilot P lant t o d evelop a n itrate s alt b earing w aste c ontainer i solation plan f or t he expedited c losure o f Panel 6 a nd Room 7 o f Panel 7 . T he D epartment i s c ommitted t o p rotecting t he health a nd s afety o f o ur w orkers a t W IPP, t he p ublic a nd t he e nvironment a s w e w ork t o u nderstand t

  19. WIPP Update 7_18_14

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    J uly 1 8, 2014 WIPP t o r educe t ransportation c ontract d rivers Visionary S olutions, a c arrier c ontracted t o t ransport w aste t o W IPP, w ill r educe i ts d river t eams a s a r esult o f t he February e vents t hat s uspended w aste d isposal o perations a t t he f acility. T he t emporary h alt i n s hipments t o WIPP h as s ignificantly r educed t he n eed f or t ransportation s ervices i n t he n ear---term. In a nticipation o f t he d ecline i n s hipments, t he C arlsbad F ield O

  20. Y-12 "Alpha" building | Y-12 National Security Complex

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Model Y ear 2 015: A lternative F uel a nd A dvanced T echnology V ehicles 1 (Updated 1 1/24/2014) 1 Source:http/ V ehicle S earch MY Fuel/Powertrain T ype Make Model Vehicle T ype Engine S ize/Cylinders Transmission Emissions C lass 2 Fuel E conomy Gasoline 3,4 City/Hwy Fuel E conomy A lt Fuel 3,4 City/Hwy All E lectric Range ( mi) 2015 B20 Chevrolet Cruze Sedan 2.0L I 4 diesel Auto Tier I I B in 5 LEV I II L EV160 27/46 N/A N/A 2015 B20 Chevrolet

  1. Lisa Gerhardt! NERSC User Services Group! NUG Training!

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    February 23, 2015 Data Management at NERSC Where Do I Put My Data? --- 2 --- * Overview o f N ERSC fi le s ystems - Local v s. G lobal - Permanent v s. P urged - Personal v s. S hared * HPSS A rchive S ystem - What i s i t a nd h ow t o u se i t * Data S haring NERSC File Systems --- 3 --- The compute and storage systems 2015 Produc=on C lusters Carver, P DSF, J GI, MatComp, Planck /global/ scratch 4 PB /project 5 PB /home 250 TB 70 P B s tored, 2 40 P B capacity, 4 0 y ears o f community d ata

  2. 01-Gerber-ASCR_Review_Overview.pptx

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Director! User Services Group Lead Review Goals & Process --- 1 --- January 1 5, 2 014 Accelerating Scientific Discovery --- 2 --- We l ove science! NERSC h olds P rogram R equirements Reviews s o w e c an p rovide f acili?es a nd services t hat b est e nable s cien?fic discovery. We're h ear t o l isten t o y our n eeds a nd help g uide y ou t o e xpress t hem a s requirements. 17 j ournal c overs i n 2 013 ASCR's Mission --- 3 --- The m ission o f t he A dvanced S cien?fic C ompu?ng R

  3. Microsoft Word - Tribal Call Jan 17 Summary 1-25-07 wep

    Office of Environmental Management (EM)

    Tri---State's s ervice a rea includes parts of Fannin County, G eorgia; P olk County, T ennessee; a nd Cherokee County, N orth C arolina. Smarter M eters H elp C ustomers B udget E lectric S ervice C osts T ri---State E lectric M embership C ooperative ( Tri---State) i s a d istribution r ural e lectric c ooperative t hat primarily s erves m ore t han 1 2,000 r ural c ustomers, m any o f w hom h ave l ow---incomes l iving a t o r n ear poverty l evel a cross a m ulti---state r egion ( see m ap).

  4. Operation Greenhouse. Scientific Director's report of atomic weapon tests at Eniwetok, 1951. Annex 2. 9. Blast injuries in foxholes

    SciTech Connect (OSTI)

    Talbot, J.M.; Maupin, C.S.


    This experiment was conducted to gain information about the amount of protection from direct blast effects that may be provided by foxholes of uniform dimensions located within distances of a nuclear explosion that are recognized as lethal for combinations of thermal and ionzing radiations and indirect blast injuries. Sixteen dogs protected in foxholes were exposed in pairs to the nuclear detonation. Autopsies performed between 10 and 15 hours after the blast demonstrated mild to moderately severe lung hemorrhages and three instances of mild to moderately severe brain hemorrhage. Ruptured ear drums and blast damage to abdominal viscera were infrequent. Evidences of acute ionizing radiation injury consisted in decreases in absolute lymphocyte counts and changes in lymph nodes and spleens. Photographs and diagrams of foxholes, animals, and tissue speciments; graphs of blast pressures, gamma doses, and neutron fluxes are included.

  5. Jason Hick! Storage Systems Group NERSC User Group Storage Update

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    NERSC User Group Storage Update Feb 2 6, 2 014 The compute and storage systems 2014 Sponsored C ompute S ystems Carver, P DSF, J GI, K BASE, H EP 8 x F DR I B /global/ scratch 4 PB /project 5 PB /home 250 TB 45 P B s tored, 2 40 P B capacity, 4 0 y ears o f community d ata HPSS 48 GB/s 2.2 P B L ocal Scratch 70 GB/s 6.4 P B L ocal Scratch 140 GB/s 80 GB/s Ethernet & I B F abric Science F riendly S ecurity ProducKon M onitoring Power E fficiency WAN 2 x 10 Gb 1 x 100 Gb Science D ata N etwork

  6. Jay Srinivasan! Group Lead, Computational Systems!

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Group Lead, Computational Systems! NUG - Feb 2015 Computational Systems Update NERSC - 2014 --- 2 --- Sponsored C ompute S ystems Carver, P DSF, J GI, K BASE, H EP 8 x F DR I B /global/ scratch 4 PB /project 5 PB /home 250 TB 45 P B s tored, 2 40 P B capacity, 4 0 y ears o f community d ata HPSS 48 GB/s 2.2 P B L ocal Scratch 70 GB/s 6.4 P B L ocal Scratch 140 GB/s 80 GB/s 2 x 10 Gb 1 x 100 Gb Science D ata N etwork Vis & A naly?cs, D ata T ransfer N odes, Adv. A rch., S cience G ateways 80

  7. OFA2013_Storage@Distance.pptx

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    NERSC Storage Systems Group Storage at a Distance --- 1 --- Open F abrics A lliance U ser D ay What is storage at a distance? * Data i s n ot l ocal t o t he u ser/resource * Processing a nd w orkflow n eeds a re n ear r eal---7me - Don't w ant t o w ait u n9l d ata t ransfer i s c omplete - Need t o s ee r esults, m ake a djustments, a nd t ry a gain * Network w ill b ecome p art o f t he i nstruments - Telescopes a nd t heir d ata - Sequencers a nd t heir g enome d ata - Light s ources a nd t

  8. Slide 1

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Min iBooNE Motivation Ex p erim ent Physics Sum m ary Kend all Mahn Colu m b ia Universit y CAM 2 005 We see t h e n um b er of n eut r in os w e ex p ect , just n ot t h e f lavor w e ex p ect Nu m b er of  e less t han ex p ect ed f rom t he su n b u t t ot al nu m b er correct  µ f rom at m osp here d isap p earing,  e am ou nt correct Accelerat or  µ , react or  e b ehave sim iliarly Neut r in o Oscillat ion s Th e cur r en t ex p lan at ion is f lavor oscillat ion s 2 f

  9. Rapid and noncontact photoacoustic tomography imaging system using an interferometer with high-speed phase modulation technique

    SciTech Connect (OSTI)

    Liu, Jun; Tang, Zhilie; Wu, Yongbo; Wang, Yi


    We designed, fabricated, and tested a rapid and noncontact photoacoustic tomography (PAT) imaging system using a low-coherence interferometer with high-speed phase modulation technique. Such a rapid and noncontact probing system can greatly decrease the time of imaging. The proposed PAT imaging system is experimentally verified by capturing images of a simulated tissue sample and the blood vessels within the ear flap of a mouse (pinna) in vivo. The axial and lateral resolutions of the system are evaluated at 45 and ∼15 μm, respectively. The imaging depth of the system is 1 mm in a special phantom. Our results show that the proposed system opens a promising way to realize noncontact, real-time PAT.

  10. Risk of Salivary Gland Cancer After Childhood Cancer: A Report From the Childhood Cancer Survivor Study

    SciTech Connect (OSTI)

    Boukheris, Houda; Stovall, Marilyn; Gilbert, Ethel S.; Stratton, Kayla L.; Smith, Susan A.; Weathers, Rita; Hammond, Sue; Mertens, Ann C.; Donaldson, Sarah S.; Armstrong, Gregory T.; Robison, Leslie L.; Neglia, Joseph P.; Inskip, Peter D.


    Purpose: To evaluate effects of radiation therapy, chemotherapy, cigarette smoking, and alcohol consumption on the risk of second primary salivary gland cancer (SGC) in the Childhood Cancer Survivor Study (CCSS). Methods and Materials: Standardized incidence ratios (SIR) and excess absolute risks (EAR) of SGC in the CCSS were calculated using incidence rates from Surveillance, Epidemiology, and End Results population-based cancer registries. Radiation dose to the salivary glands was estimated based on medical records. Poisson regression was used to assess risks with respect to radiation dose, chemotherapy, smoking, and alcohol consumption. Results: During the time period of the study, 23 cases of SGC were diagnosed among 14,135 childhood cancer survivors. The mean age at diagnosis of the first primary cancer was 8.3 years, and the mean age at SGC diagnosis was 24.8 years. The incidence of SGC was 39-fold higher in the cohort than in the general population (SIR = 39.4; 95% CI = 25.4-57.8). The EAR was 9.8 per 100,000 person-years. Risk increased linearly with radiation dose (excess relative risk = 0.36/Gy; 95% CI = 0.06-2.5) and remained elevated after 20 years. There was no significant trend of increasing risk with increasing dose of chemotherapeutic agents, pack-years of cigarette smoking, or alcohol intake. Conclusion: Although the cumulative incidence of SGC was low, childhood cancer survivors treated with radiation experienced significantly increased risk for at least 2 decades after exposure, and risk was positively associated with radiation dose. Results underscore the importance of long-term follow up of childhood cancer survivors for the development of new malignancies.

  11. Characterization of aerosols produced by surgical procedures

    SciTech Connect (OSTI)

    Yeh, H.C.; Muggenburg, B.A.; Lundgren, D.L.; Guilmette, R.A.; Snipes, M.B.; Jones, R.K.; Turner, R.S.


    In many surgeries, especially orthopedic procedures, power tools such as saws and drills are used. These tools may produce aerosolized blood and other biological material from bone and soft tissues. Surgical lasers and electrocautery tools can also produce aerosols when tissues are vaporized and condensed. Studies have been reported in the literature concerning production of aerosols during surgery, and some of these aerosols may contain infectious material. Garden et al. (1988) reported the presence of papilloma virus DNA in the fumes produced from laser surgery, but the infectivity of the aerosol was not assessed. Moon and Nininger (1989) measured the size distribution and production rate of emissions from laser surgery and found that particles were generally less than 0.5 {mu}m diameter. More recently there has been concern expressed over the production of aerosolized blood during surgical procedures that require power tools. In an in vitro study, the production of an aerosol containing the human immunodeficiency virus (HIV) was reported when power tools were used to cut tissues with blood infected with HIV. Another study measured the size distribution of blood aerosols produced by surgical power tools and found blood-containing particles in a number of size ranges. Health care workers are anxious and concerned about whether surgically produced aerosols are inspirable and can contain viable pathogens such as HIV. Other pathogens such as hepatitis B virus (HBV) are also of concern. The Occupational Safety and Health funded a project at the National Institute for Inhalation Toxicology Research Institute to assess the extent of aerosolization of blood and other tissues during surgical procedures. This document reports details of the experimental and sampling approach, methods, analyses, and results on potential production of blood-associated aerosols from surgical procedures in the laboratory and in the hospital surgical suite.

  12. Fibroblast growth factor 2 inhibits up-regulation of bone morphogenic proteins and their receptors during osteoblastic differentiation of human mesenchymal stem cells

    SciTech Connect (OSTI)

    Biver, Emmanuel; Soubrier, Anne-Sophie; Thouverey, Cyril; Cortet, Bernard; Broux, Odile; Caverzasio, Joseph; Hardouin, Pierre


    Highlights: Black-Right-Pointing-Pointer FGF modulates BMPs pathway in HMSCs by down-regulating BMP/BMPR expression. Black-Right-Pointing-Pointer This effect is mediated by ERK and JNK MAPKs pathways. Black-Right-Pointing-Pointer Crosstalk between FGF and BMPs must be taken into account in skeletal bioengineering. Black-Right-Pointing-Pointer It must also be considered in the use of recombinant BMPs in orthopedic and spine surgeries. -- Abstract: Understanding the interactions between growth factors and bone morphogenic proteins (BMPs) signaling remains a crucial issue to optimize the use of human mesenchymal stem cells (HMSCs) and BMPs in therapeutic perspectives and bone tissue engineering. BMPs are potent inducers of osteoblastic differentiation. They exert their actions via BMP receptors (BMPR), including BMPR1A, BMPR1B and BMPR2. Fibroblast growth factor 2 (FGF2) is expressed by cells of the osteoblastic lineage, increases their proliferation and is secreted during the healing process of fractures or in surgery bone sites. We hypothesized that FGF2 might influence HMSC osteoblastic differentiation by modulating expressions of BMPs and their receptors. BMP2, BMP4, BMPR1A and mainly BMPR1B expressions were up-regulated during this differentiation. FGF2 inhibited HMSCs osteoblastic differentiation and the up-regulation of BMPs and BMPR. This effect was prevented by inhibiting the ERK or JNK mitogen-activated protein kinases which are known to be activated by FGF2. These data provide a mechanism explaining the inhibitory effect of FGF2 on osteoblastic differentiation of HMSCs. These crosstalks between growth and osteogenic factors should be considered in the use of recombinant BMPs in therapeutic purpose of fracture repair or skeletal bioengineering.

  13. Microstructural characterization of as-cast biocompatible Co-Cr-Mo alloys

    SciTech Connect (OSTI)

    Giacchi, J.V.; Morando, C.N.; Fornaro, O.; Palacio, H.A.


    The microstructure of a cobalt-base alloy (Co-Cr-Mo) obtained by the investment casting process was studied. This alloy complies with the ASTM F75 standard and is widely used in the manufacturing of orthopedic implants because of its high strength, good corrosion resistance and excellent biocompatibility properties. This work focuses on the resulting microstructures arising from samples poured under industrial environment conditions, of three different Co-Cr-Mo alloys. For this purpose, we used: 1) an alloy built up from commercial purity constituents, 2) a remelted alloy and 3) a certified alloy for comparison. The characterization of the samples was achieved by using optical microscopy (OM) with a colorant etchant to identify the present phases and scanning electron microscopy (SE-SEM) and energy dispersion spectrometry (EDS) techniques for a better identification. In general the as-cast microstructure is a Co-fcc dendritic matrix with the presence of a secondary phase, such as the M{sub 23}C{sub 6} carbides precipitated at grain boundaries and interdendritic zones. These precipitates are the main strengthening mechanism in this type of alloys. Other minority phases were also reported and their presence could be linked to the cooling rate and the manufacturing process variables and environment. - Research Highlights: {yields}The solidification microstructure of an ASTM-F75 type alloy were studied. {yields}The alloys were poured under an industrial environment. {yields}Carbides and sigma phase identified by color metallography and scanning microscopy (SEM and EDS). {yields}Two carbide morphologies were detected 'blocky type' and 'pearlite type'. {yields}Minority phases were also detected.

  14. Selenium Preferentially Accumulates in the Eye Lens Following Embryonic Exposure: A Confocal X-ray Fluorescence Imaging Study

    SciTech Connect (OSTI)

    Choudhury, Sanjukta; Thomas, Jith; Sylvain, Nicole J.; Ponomarenko, Olena; Gordon, Robert A.; Heald, Steve M.; Janz, David M.; Krone, Patrick H.; Coulthard, Ian; George, Graham N.; Pickering, Ingrid J.


    Maternal transfer of elevated selenium (Se) to offspring is an important route of Se exposure for fish in the natural environment. However, there is a lack of information on the tissue specific spatial distribution and speciation of Se in the early developmental stages of fish, which provide important information about Se toxicokinetics. The effect of maternal transfer of Se was studied by feeding adult zebrafish a Se-elevated or a control diet followed by collection of larvae from both groups. Novel confocal synchrotron-based techniques were used to investigate Se within intact preserved larvae. Confocal X-ray fluorescence imaging was used to compare Se distributions within specific planes of an intact larva from each of the two groups. The elevated Se treatment showed substantially higher Se levels than the control; Se preferentially accumulated to highest levels in the eye lens, with lower levels in the retina, yolk and other tissues. Confocal X-ray absorption spectroscopy was used to determine that the speciation of Se within the eye lens of the intact larva was a selenomethionine-like species. Preferential accumulation of Se in the eye lens may suggest a direct cause-and-effect relationship between exposure to elevated Se and Se-induced ocular impairments reported previously. This study illustrates the effectiveness of confocal X-ray fluorescence methods for investigating trace element distribution and speciation in intact biological specimens

  15. Etiology of Early Lifestage Diseases, Project 84-44, 1985 Final Report.

    SciTech Connect (OSTI)

    Sauter, R.W.


    Each year hatcheries experience loss of eggs, fry and fingerlings due to a group of poorly defined diseases called White Spot and Coagulated Yolk. Samples of maternal blood and ovarian fluid (coelomic fluid), as well as unfertilized eggs, were collected at spawning and subsequently tested for the presence of bacteria. Our tests reveal that there is a wide range in mortality rates experienced by the progeny of different brood salmon. Microbiologic tests revealed that these eggs contained a variety of both Gram positive and Gram negative bacteria within their yolk and that the fluids from the females who produced these lots were contaminated with a variety of bacteria. In contrast the eggs and maternal fluids from the six egg lots which experienced the lowest mortalities did not contain high numbers of Gram positive bacteria and contributed only 5% of the total mortalities observed within the 30 egg lots tested. From the 60 egg lots tested over two brood years we have isolated 18 different bacterial genera containing 32 different species from within the yolk surface sterilized, unfertilized eggs. Our tests suggest that Aeromonas hydrophila, Pseudomonas (3 species) Staphylococcus aureus, Vibrio sp., Corynebacterium hoffmanii, Listeria sp. and Bacillus sp. when detected within the yolk of eggs sampled from egg lots prior to fertilization will be associated with higher than normal mortality rates when the remainder of the egg lots containing these bacteria are incubated and reared. 36 refs., 16 figs.

  16. Glomerular-specific imprinting of the mouse Gs{alpha} gene: How does this relate to hormone resistance in Albright hereditary osteodystrophy?

    SciTech Connect (OSTI)

    Williamson, C.M.; Dutton, E.R.; Seymour, A.


    The gene for alpha-stimulating guanine-nucleotide binding polypeptide, Gnas, has been considered as a candidate for the imprinting effects ascribed to distal mouse Chromosome (Chr) 2. Its human homologue (GNAS1) appears, from clinical and biochemical studies of patients with Albright hereditary ostodystrophy, to be paternally imprinted. GNAS1 maps to 20q13, a region that shows linkage conservation with distal mouse Chr 2. We have mapped Gnas within the imprinting region on distal Chr 2 by linkage analysis. To establish if Gnas is imprinted, we have looked for expression differences in tissues taken from mice carrying maternal duplication/paternal deficiency for distal Chr 2 (MatDp2) and its reciprocal (PatDp2). RNA in situ hybridization revealed high levels of Gnas mRNA in glomeruli of PatDp2 embryos at late gestation and lower levels in glomeruli of MatDp2 embryos. These results strongly suggest that Gnas is maternally imprinted and suggest that the mouse gene may be imprinted in a manner opposite the predicted in human. 42 refs., 4 figs.

  17. Inhalation developmental toxicology studies: Developmental toxicity of chloroprene vapors in New Zealand white rabbits. Final report

    SciTech Connect (OSTI)

    Mast, T.J.; Evanoff, J.J.; Westerberg, R.B.; Rommereim, R.L.; Weigel, R.J.


    Chloroprene, 2-chloro-1,3-butadiene, is a colorless liquid with a pungent ethereal odor that is primarily used as an intermediate in the manufacture of neoprene rubber, and has been used as such since about 1930. This study addressed the potential for chloroprene to cause developmental toxicity in New Zealand white rabbits following gestational exposure to 0, 10, 40, or 175 ppm chloroprene vapors, 6h/dy, 7dy/wk. Each treatment group consisted of 15 artificially inseminated females exposed on 6 through 28 days of gestation (dg). Body weights were obtained throughout the study period, and uterine and fetal body weights were obtained at sacrifice on 29 dg. Implants were enumerated and their status recorded and live fetuses were examined for gross, visceral, skeletal, and soft-tissue craniofacial defects. There were no overt signs of maternal toxicity and the change in maternal body weight over the course of the study was not affected. Exposure of pregnant rabbits to chloroprene vapors on 6-28 dg had no effect on the number of implantation, the mean percent of live pups per litter, or on the incidence of resorptions per litter. The incidence of fetal malformations was not increased by exposure to chloroprene. Results of this study indicate that gestational exposure of New Zealand white rabbits to 10, 40, or 175 ppm chloroprene did not result in observable toxicity to either the dam or the offspring.

  18. Inhalation developmental toxicology studies: Acetonitrile in rats. Final report

    SciTech Connect (OSTI)

    Mast, T.J.; Weigel, R.J.; Westerberg, R.B.; Boyd, P.J.; Hayden, B.K.; Evanoff, J.J.; Rommereim, R.L.


    The potential for acetonitrile to cause developmental toxicity was assessed in Sprague-Dawley rats exposed to 0, 100, 400, or 1200 ppM acetonitrile, 6 hours/day, 7 days/week. Exposure of rats to these concentrations of acetonitrile resulted in mortality in the 1200 ppM group (2/33 pregnant females; 1/10 non-pregnant females). However, there were no treatment-related effects upon body weights or reproduction indices at any exposure level, nor was there a significant increase in the incidence of fetal malformations or variations. The only effect observed in the fetuses was a slight, but not statiscally significant, exposure-correlated increase in the incidence of supernumerary ribs. Determination of acetonitrile and cyanide concentrations in maternal rat blood showed that acetonitrile concentration in the blood increased with exposure concentration for all exposed maternal rats. Detectable amounts of cyanide in the blood were found only in the rats exposed to 1200 ppM acetonitrile ({approximately}2 {mu}g cyanide/g of blood).

  19. The production, purification and crystallization of a soluble form of the nonclassical MHC HLA-G: the essential role of cobalt

    SciTech Connect (OSTI)

    Clements, Craig S.; Kjer-Nielsen, Lars; Kostenko, Lyudmila; McCluskey, James; Rossjohn, Jamie


    X-ray diffraction data were collected to 1.9 from crystals of HLA-G. Cobalt ions were found to be essential for the production of diffracting crystals. HLA-G is a nonclassical class I major histocompatibility complex (MHC) molecule that is primarily expressed at the foetalmaternal interface. Although the role of HLA-G has not been fully elucidated, current evidence suggests it protects the foetus from the maternal immune response. In this report, HLA-G (44 kDa) is characterized by expression in Escherichia coli. The inclusion bodies were refolded in complex with a peptide derived from histone H2A (RIIPRHLQL), purified and subsequently crystallized. Correct refolding was determined using two conformation-dependent antibodies. Cobalt ions were shown to be an essential ingredient for obtaining diffraction-quality crystals. The crystals, which diffracted to 1.9 resolution, belonged to space group P3{sub 2}2{sub 1}, with unit-cell parameters a = b = 77.15, c = 151.72 .

  20. Final report on the developmental toxicity of ethylenediamine (CAS No. 107-15-3) in New Zealand white rabbits. Report for November-February 1992

    SciTech Connect (OSTI)

    Not Available


    Ethylenediamine (EDA) is a major industrial chemical with an estimated U.S. production of 64 million pounds in 1985. EDA is used as a chemical intermediate or solvent in manufacturing, as a paint thinner, and as a constituent of certain cosmetic, pharmaceutical and veterinary products. Based upon its widespread applications and the potential for exposure in pregnant women, EDA was evaluated for maternal and developmental toxicity using a laboratory animal model. Artificially-inseminated New Zealand White rabbits (26/group) were administered ethylenediamine (0, 10, 40 or 80 mg/kg/day) by gavage on gestational days (gd) 6 through 19. The doses administered were equivalent to 0, 22, 89 or 178 of EDA.2HC1. Maternal clinical signs, body weight, and food consumption were monitored at regular intervals throughout gestation. At termination (gd 30), the uterus was removed and examined to determine pregnancy status and to evaluate the number of resorptions, and dead or live fetuses. Dead or live fetuses were weighed, and live fetuses examined for external, visceral and skeletal defects.

  1. Second Malignancies After Adjuvant Radiation Therapy for Early Stage Breast Cancer: Is There Increased Risk With Addition of Regional Radiation to Local Radiation?

    SciTech Connect (OSTI)

    Hamilton, Sarah Nicole; Tyldesley, Scott; Li, Dongdong; Olson, Robert; McBride, Mary


    Purpose: This study was undertaken to determine whether there was an increased risk of second malignancies (SM), particularly lung cancer, in early stage breast cancer patients treated with the addition of nodal fields to breast and/or chest wall radiation therapy (RT). Materials and Methods: Subjects were stage I/II female breast cancer patients 20 to 79 years of age, diagnosed between 1989 and 2005 and treated with adjuvant RT at our institution. Patients were included if they survived and did not have SM within 3 years of diagnosis. Standardized incidence ratios (SIR) with 95% confidence intervals (CI) were calculated to compare SM incidence to cancer incidence in the general sex- and age-matched populations. Secondary malignancy risks in patients treated with local RT (LRT) to the breast/chest wall were compared to those in patients treated with locoregional RT (LRRT) to the breast/chest wall and regional nodes, using multivariate regression analysis (MVA) to account for covariates. Results: The cohort included 12,836 patients with a median follow-up of 8.4 years. LRRT was used in 18% of patients. The SIR comparing patients treated with LRT to the general population was 1.29 (CI: 1.21-1.38). No statistically significant increased incidence of in-field malignancies (SIR, 1.04; CI: 0.87-1.23) and lung cancers (SIR, 1.06; CI: 0.88-1.26) was detected. The SIR comparing patients treated with LRRT to the general population was 1.39 (CI: 1.17-1.64). No statistically significant increased incidence of in-field malignancies (SIR, 1.26; CI: 0.77-1.94) and lung cancers (SIR, 1.27; CI: 0.76-1.98) was detected. On MVA comparing LRRT to LRT, the adjusted hazard ratio was 1.20 for in-field malignancies (CI: 0.68-2.16) and 1.26 for lung cancer (CI: 0.67-2.36). The excess attributable risk (EAR) to regional RT was 3.1 per 10,000 person years (CI: −8.7 to 9.9). Conclusions: No statistically significant increased risk of second malignancy was detected after LRRT relative to

  2. Infant sex-specific placental cadmium and DNA methylation associations

    SciTech Connect (OSTI)

    Mohanty, April F.; Farin, Fred M.; Bammler, Theo K.; MacDonald, James W.; Afsharinejad, Zahra; Burbacher, Thomas M.; Siscovick, David S.; and others


    Background: Recent evidence suggests that maternal cadmium (Cd) burden and fetal growth associations may vary by fetal sex. However, mechanisms contributing to these differences are unknown. Objectives: Among 24 maternal-infant pairs, we investigated infant sex-specific associations between placental Cd and placental genome-wide DNA methylation. Methods: We used ANOVA models to examine sex-stratified associations of placental Cd (dichotomized into high/low Cd using sex-specific Cd median cutoffs) with DNA methylation at each cytosine-phosphate-guanine site or region. Statistical significance was defined using a false discovery rate cutoff (<0.10). Results: Medians of placental Cd among females and males were 5 and 2 ng/g, respectively. Among females, three sites (near ADP-ribosylation factor-like 9 (ARL9), siah E3 ubiquitin protein ligase family member 3 (SIAH3), and heparin sulfate (glucosamine) 3-O-sulfotransferase 4 (HS3ST4) and one region on chromosome 7 (including carnitine O-octanoyltransferase (CROT) and TP5S target 1 (TP53TG1)) were hypomethylated in high Cd placentas. Among males, high placental Cd was associated with methylation of three sites, two (hypomethylated) near MDS1 and EVI1 complex locus (MECOM) and one (hypermethylated) near spalt-like transcription factor 1 (SALL1), and two regions (both hypomethylated, one on chromosome 3 including MECOM and another on chromosome 8 including rho guanine nucleotide exchange factor (GEF) 10 (ARHGEF10). Differentially methylated sites were at or close to transcription start sites of genes involved in cell damage response (SIAH3, HS3ST4, TP53TG1) in females and cell differentiation, angiogenesis and organ development (MECOM, SALL1) in males. Conclusions: Our preliminary study supports infant sex-specific placental Cd-DNA methylation associations, possibly accounting for previously reported differences in Cd-fetal growth associations across fetal sex. Larger studies are needed to replicate and extend these

  3. Method and apparatus for non-invasive monitoring of blood glucose

    DOE Patents [OSTI]

    Thomas, Graham H.; Watson, Roger M.; Noell, J. Oakey


    A new and improved method and apparatus are provided for non-invasive monitoring of changes in blood glucose concentration in a tissue specimen and particularly in an individual. The method uses acoustic velocity measurements for monitoring the effect of glucose concentration upon the density and adiabatic compressibility of the serum. In a preferred embodiment, the acoustic velocity measurements are made through the earlobe of a subject by means of an acoustic probe or monitor which includes a transducer for transmitting and receiving ultrasonic energy pulses to and from the blood flowing in the subject's earlobe and a reflector for facilitating reflection of the acoustic pulses from the blood. The probe is designed in such a way that when properly affixed to an ear, the transducer is positioned flush against the anterior portion of an earlobe while the reflector is positioned flush against the interior portion of the earlobe. A microthermocouple is provided on the probe for monitoring the internal temperature of the blood being sampled. An electrical system, essentially comprising a frequency generator, a time intervalometer and an oscilloscope, is linked to the glucose monitoring probe. The electrical system analyzes selected ones of the pulses reflected from the blood sample in order to determine therefrom the acoustic velocity of the blood which, in turn, provides a representation of the blood glucose concentration levels at the time of the acoustic velocity measurements.

  4. Generation of insulin-producing cells from gnotobiotic porcine skin-derived stem cells

    SciTech Connect (OSTI)

    Yang, Ji Hoon; Lee, Sung Ho; Heo, Young Tae; Uhm, Sang Jun; Lee, Hoon Taek


    A major problem in the treatment of type 1 diabetes mellitus is the limited availability of alternative sources of insulin-producing cells for islet transplantation. In this study, we investigated the effect of bone morphogenetic protein 4 (BMP-4) treatments of gnotobiotic porcine skin-derived stem cells (gSDSCs) on their reprogramming and subsequent differentiation into insulin-producing cells (IPCs). We isolated SDSCs from the ear skin of a gnotobiotic pig. During the proliferation period, the cells expressed stem-cell markers Oct-4, Sox-2, and CD90; nestin expression also increased significantly. The cells could differentiate into IPCs after treatments with activin-A, glucagon-like peptide-1 (GLP-1), and nicotinamide. After 15 days in the differentiation medium, controlled gSDSCs began expressing endocrine progenitor genes and proteins (Ngn3, Neuro-D, PDX-1, NKX2.2, NKX6.1, and insulin). The IPCs showed increased insulin synthesis after glucose stimulation. The results indicate that stem cells derived from the skin of gnotobiotic pigs can differentiate into IPCs under the appropriate conditions in vitro. Our three-stage induction protocol could be applied without genetic modification to source IPCs from stem cells in the skin of patients with diabetes for autologous transplantation.

  5. Use of non-quadratic yield surfaces in design of optimal deep-draw blank geometry

    SciTech Connect (OSTI)

    Logan, R.W.


    Planar anisotropy in the deep-drawing of sheet can lead to the formation of ears in cylindrical cups and to undesirable metal flow in the blankholder in the general case. For design analysis purposes in non-linear finite-element codes, this anisotropy is characterized by the use of an appropriate yield surface which is then implemented into codes such as DYNA3D . The quadratic Hill yield surface offers a relatively straightforward implementation and can be formulated to be invariant to the coordinate system. Non-quadratic yield surfaces can provide more realistic strength or strain increment ratios, but they may not provide invariance and thus demand certain approximations. Forms due to Hosford and Badat et al. have been shown to more accurately address the earning phenomenon. in this work, use is made of these non-quadratic yield surfaces in order to determine the optimal blank shape for cups and other shapes using ferrous and other metal blank materials with planar anisotropy. The analyses are compared to previous experimental studies on non-uniform blank motion due to anisotropy and asymmetric geometry.

  6. AmeriFlux US-Ton Tonzi Ranch

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Baldocchi, Dennis [University of California, Berkeley


    This is the AmeriFlux version of the carbon flux data for the site US-Ton Tonzi Ranch. Site Description - Located in the lower foothills of the Sierra Nevada Mountains, the Tonzi Ranch site is classified as an oak savanna woodland on privately owned land. Managed by local rancher, Russell Tonzi, brush has been periodically removed for cattle grazing. The overstory is dominated by blue oak trees (40% of total vegetation) with intermittent grey pine trees (3 trees/ha). Understory species include a variety of grasses and herbs, including purple false brome, smooth cat's ear, and rose clover. These two distinctive layers operate in and out from one another. Growing season of the understory is confined to the wet season only, typically from October to early May. In contrast, the deciduous blue oak trees are dormant during the rainy winter months and reach maximum LAI in April. The blue oak ecosystem rings the Great Central Valley of California, inhabiting the lower reaches of the Sierra Nevada foothills.

  7. The Rosetta Resources CO2 Storage Project - A WESTCARB GeologicPilot Test

    SciTech Connect (OSTI)

    Trautz, Robert; Benson, Sally; Myer, Larry; Oldenburg, Curtis; Seeman, Ed; Hadsell, Eric; Funderburk, Ben


    WESTCARB, one of seven U.S. Department of Energypartnerships, identified (during its Phase I study) over 600 gigatonnesof CO2 storage capacity in geologic formations located in the Westernregion. The Western region includes the WESTCARB partnership states ofAlaska, Arizona, California, Nevada, Oregon and Washington and theCanadian province of British Columbia. The WESTCARB Phase II study iscurrently under way, featuring three geologic and two terrestrial CO2pilot projects designed to test promising sequestration technologies atsites broadly representative of the region's largest potential carbonsinks. This paper focuses on two of the geologic pilot studies plannedfor Phase II -referred to-collectively as the Rosetta-Calpine CO2 StorageProject. The first pilot test will demonstrate injection of CO2 into asaline formation beneath a depleted gas reservoir. The second test willgather data for assessing CO2 enhanced gas recovery (EGR) as well asstorage in a depleted gas reservoir. The benefit of enhanced oil recovery(EOR) using injected CO2 to drive or sweep oil from the reservoir towarda production well is well known. EaR involves a similar CO2 injectionprocess, but has received far less attention. Depleted natural gasreservoirs still contain methane; therefore, CO2 injection may enhancemethane production by reservoir repressurization or pressure maintenance.CO2 injection into a saline formation, followed by injection into adepleted natural gas reservoir, is currently scheduled to start inOctober 2006.

  8. Impact of TBI on late effects in children treated by megatherapy for Stage IV neuroblastoma. A study of the French Society of Pediatric oncology

    SciTech Connect (OSTI)

    Flandin, Isabelle; Michon, Jean; Pinkerton, Ross; Coze, Carole; Stephan, Jean Louis; Fourquet, Bernard; Valteau-Couanet, Dominique; Bergeron, Christophe; Philip, Thierry; Carrie, Christian . E-mail:


    Purpose: To determine the contribution of total body irradiation (TBI) to late sequelae in children treated with high-dose chemotherapy and autologous bone marrow transplantation for Stage IV neuroblastoma. Patients and Methods: We compared two populations that were similar with regard to age, stage, pre-autologous bone marrow transplantation chemotherapy (CT) regimen, period of treatment, and follow-up (12 years). The TBI group (n = 32) received TBI as part of the megatherapy procedure (1982-1993), whereas the CT group (n 30) received conditioning without TBI (1985-1992). Analysis 12 years later focused on growth, weight and corpulence (body mass index) delay; hormonal deficiencies; liver, kidney, heart, ear, eye, and dental sequelae; school performance; and the incidence of secondary tumors. Results: Impact of TBI was most marked in relation to growth and weight delay, although the mean delay was not severe, probably because of treatment with growth hormones. Other consequences of TBI were thyroid insufficiency, cataracts, and a high incidence of secondary tumors. Hearing loss and dental agenesis were more prominent in the group treated with CT alone. No differences were observed in school performance. Conclusion: The most frequent side effects of TBI were cataracts, thyroid insufficiency, and growth delay, but more worrying is the risk of secondary tumors. Because of the young mean age of patients and the toxicity of TBI regimens without any survival advantage, regimens without TBI are preferable in the management of Stage IV neuroblastoma.

  9. Vertical distribution of structural components in corn stover


    Johnson, Jane M. F.; Karlen, Douglas L.; Gresham, Garold L.; Cantrell, Keri B.; Archer, David W.; Wienhold, Brian J.; Varvel, Gary E.; Laird, David A.; Baker, John; Ochsner, Tyson E.; et al


    In the United States, corn (Zea mays L.) stover has been targeted for second generation fuel production and other bio-products. Our objective was to characterize sugar and structural composition as a function of vertical distribution of corn stover (leaves and stalk) that was sampled at physiological maturity and about three weeks later from multiple USA locations. A small subset of samples was assessed for thermochemical composition. Concentrations of lignin, glucan, and xylan were about 10% greater at grain harvest than at physiological maturity, but harvestable biomass was about 25% less due to stalk breakage. Gross heating density above the earmore » averaged 16.3 ± 0.40 MJ kg⁻¹, but with an alkalinity measure of 0.83 g MJ⁻¹, slagging is likely to occur during gasification. Assuming a stover harvest height of 10 cm, the estimated ethanol yield would be >2500 L ha⁻¹, but it would be only 1000 L ha⁻¹ if stover harvest was restricted to the material from above the primary ear. Vertical composition of corn stover is relatively uniform; thus, decision on cutting height may be driven by agronomic, economic and environmental considerations.« less

  10. GPR Imaging of Clastic Dikes at the Hanford Site, Hanford, Washington

    SciTech Connect (OSTI)

    Clement, William P.; Murray, Christopher J.


    We use ground penetrating radar (GPR) data to help determine the spatial distribution and the subsurface geometry of clastic injection dikes at the Hanford site. This information will help to improve the understanding of the hydrological role of these ubiquitous clastic dikes at the Han¬ford Site. We collected 100 MHz ground penetrating radar (GPR) 3D surface reflection data at two sites, the S-16 Pond and the Army Loop Road sites, and 2D reflection data along a 6.9 km lin¬ear transect near the Army Loop Road site. The dikes are distinguished in the GPR data by a strongly attenuated zone, disruptions in the continuity of reflections, and diffractions where reflections are disrupted. In general, the data quality is better at the Army Loop Road and Traverse sites than at the S-16 Pond site, probably due to the presence of cobbles at the S-16 Pond site. A high-moisture, fine-grained unit probably causes the strong reflections at the Army Loop Road site and the Traverse survey site. The signal penetration varies between 5 to 12 m below the land surface.

  11. Methane emission from single cropping rice paddies amended different manures

    SciTech Connect (OSTI)

    Du Daodeng; Tao Zhan


    Methane emission fluxes were determined from single cropping rice paddies amended with different manures through a productively comparative experiment. The average fluxes in the whole growth season ranged from 3.92 to 10.96 mg/m{sup 2}.hr. The compost amended paddies gave the highest emission fluxes of 10.26 mg/m{sup 2}.hr, while the fluxes from the other manure amended paddies ranked as follows: horse dung biogas digester sediment 10.02, chemical fertilizer only 8.81, nightsoil biogas sediment 7.76, chicken dropping biogas digester sediment 4.48 and pig dung biogas digester sediment 3.92 mg/m{sup 2}.hr. The latter 3 sediments gave the significant less ({alpha} < 0.05) fluxes than compost. The highest fluxes peaks of all treated paddies appeared unanimously between the stages of the midtillering and the earing, with a half of total CH{sub 4} emissions were produced in this period which could be chosen as the key period for control of CH{sub 4} emission from the single cropping rice paddies. The positive correlation of the fluxes with the temperatures in 5 cm soil layers and the negative correlation of the fluxes with the rice yields, the soil N and P{sub 2}O{sub 5} contents were also observed.

  12. SU-E-T-208: Incidence Cancer Risk From the Radiation Treatment for Acoustic Neuroma Patient

    SciTech Connect (OSTI)

    Kim, D; Chung, W; Shin, D; Yoon, M


    Purpose: The present study aimed to compare the incidence risk of a secondary cancer from therapeutic doses in patients receiving intensitymodulated radiotherapy (IMRT), volumetric modulated arc therapy (VMAT), and stereotactic radiosurgery (SRS). Methods: Four acoustic neuroma patients were treated with IMRT, VMAT, or SRS. Their incidnece excess relative risk (ERR), excess absolute risk (EAR), and lifetime attributable risk (LAR) were estimated using the corresponding therapeutic doses measured at various organs by radio-photoluminescence glass dosimeters (RPLGD) placed inside a humanoid phantom. Results: When a prescription dose was delivered in the planning target volume of the 4 patients, the average organ equivalent doses (OED) at the thyroid, lung, normal liver, colon, bladder, prostate (or ovary), and rectum were measured. The OED decreased as the distance from the primary beam increased. The thyroid received the highest OED compared to other organs. A LAR were estimated that more than 0.03% of AN patients would get radiation-induced cancer. Conclusion: The tyroid was highest radiation-induced cancer risk after radiation treatment for AN. We found that LAR can be increased by the transmitted dose from the primary beam. No modality-specific difference in radiation-induced cancer risk was observed in our study.

  13. C~]

    U.S. Department of Energy (DOE) all webpages (Extended Search)

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  14. Assessment of methods and results of reproductive occupational epidemiology: spontaneous abortions and malformations in the offspring of working women

    SciTech Connect (OSTI)

    Hemminki, K.; Axelson, O.; Niemi, M.L.; Ahlborg, G.


    Epidemiological studies relating occupational exposures of working women to spontaneous abortions and malformation are reviewed and some methodological considerations are presented. The reproductive epidemiology is less developed than epidemiology in general and seems to involve some specific problems. The exposures may be reported differently by the women depending on the outcome of the pregnancy; thus confirmation of exposure from an independent data source would be an asset. The types of occupational exposures of the women, suggested to carry a risk of spontaneous abortions, include anesthetic agents, laboratory work, copper smelting, soldering, and chemical sterilization using ethylene oxide and glutaraldehyde. Maternal employment in laboratories and exposure to solvents has been linked to a risk of congenital malformations in the offspring in five studies. Data on the teratogenic effects of anesthetic gases has been conflicting. In one study, employment in copper smelting was associated with malformations in the offspring.

  15. Repeated in utero and lactational 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure affects male gonads in offspring, leading to sex ratio changes in F{sub 2} progeny

    SciTech Connect (OSTI)

    Ikeda, Masahiko . E-mail:; Tamura, Masashi; Yamashita, Junko; Suzuki, Chinatsu; Tomita, Takako


    The effects of in utero and lactational 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the reproductive system of male rat offspring (F{sub 1}) and the sex ratio of the subsequent generation (F{sub 2}) were examined. Female Holtzman rats were gavaged with an initial loading dose of 400 ng/kg TCDD prior to mating, followed by weekly maintenance doses of 80 ng/kg during mating, pregnancy, and the lactation period. Maternal exposure to TCDD had no significant effects on fetus/pup (F{sub 1}) mortality, litter size, or sex ratio on gestation day (GD) 20 or postnatal day (PND) 2. The TCDD concentration in maternal livers and adipose tissue on GD20 was 1.21 and 1.81 ng/kg, respectively, and decreased at weaning to 0.72 in the liver and 0.84 in the adipose tissue. In contrast, the TCDD concentration in pup livers was 1.32 ng/kg on PND2 and increased to 1.80 ng/kg at weaning. Ventral prostate weight of male offspring was significantly decreased by TCDD exposure on PND28 and 120 compared with that of controls. Weight of the testes, cauda epididymides, and seminal vesicle, and sperm number in the cauda epididymis were not changed by TCDD exposure at PND120. TCDD- or vehicle-exposed male offspring were mated with unexposed females. The sex ratio (percentage of male pups) of F{sub 2} offspring was significantly reduced in the TCDD-exposed group compared with controls. These results suggest that in utero and lactational TCDD exposures affect the development of male gonads in offspring (F{sub 1}), leading to changes in the sex ratio of the subsequent generation (F{sub 2})

  16. Placental concentrations of heavy metals in a mother-child cohort

    SciTech Connect (OSTI)

    Amaya, E.; Gil, F.; Freire, C.; National School of Public Health, Oswaldo Cruz Foundation , 21041-210 Rio de Janeiro ; Olmedo, P.; Fernandez-Rodriguez, M.; Fernandez, M.F.; Olea, N.; CIBER de Epidemiologia y Salud Publica


    Heavy metals are environmental contaminants with properties known to be toxic for wildlife and humans. Despite strong concerns about their harmful effects, little information is available on intrauterine exposure in humans. The aim of this study was to evaluate prenatal exposure to As, Cd, Cr, Hg, Mn, and Pb and its association with maternal factors in a population-based mother-child cohort in Southern Spain. Between 2000 and 2002, 700 pregnant women were recruited and 137 placentas from the cohort were randomly selected and analyzed for the selected metals by atomic absorption. Maternal sociodemographic and lifestyle factors were obtained by questionnaire after delivery. Bivariate analysis and multivariate linear regression were performed. Cd and Mn concentrations were detected in all placentas, while Cr, Pb, and Hg were found in 98.5%, 35.0%, and 30.7% of samples, respectively. The highest concentrations were observed for Pb (mean: 94.80 ng/g wet weight of placenta), followed by Mn (63.80 ng/g), Cr (63.70 ng/g), Cd (3.45 ng/g), and Hg (0.024 ng/g). Arsenic was not detected in any sample. Gestational age and smoking during pregnancy were associated with placental Cd concentrations, while no factor appeared to influence concentrations of Cr, Hg, Mn, or Pb. In comparison to results of European studies, these concentrations are in a low-intermediate position. Studies are required to investigate the factors contributing to early exposure to heavy metals and to determine how placental transfer of these toxic compounds may affect children's health.

  17. Remote Whispering Applying Time Reversal

    SciTech Connect (OSTI)

    Anderson, Brian Eric


    The purpose of this project was to explore the use of time reversal technologies as a means for communication to a targeted individual or location. The idea is to have the privacy of whispering in one’s ear, but to do this remotely from loudspeakers not located near the target. Applications of this work include communicating with hostages and survivors in rescue operations, communicating imaging and operational conditions in deep drilling operations, monitoring storage of spent nuclear fuel in storage casks without wires, or clandestine activities requiring signaling between specific points. This technology provides a solution in any application where wires and radio communications are not possible or not desired. It also may be configured to self calibrate on a regular basis to adjust for changing conditions. These communications allow two people to converse with one another in real time, converse in an inaudible frequency range or medium (i.e. using ultrasonic frequencies and/or sending vibrations through a structure), or send information for a system to interpret (even allowing remote control of a system using sound). The time reversal process allows one to focus energy to a specific location in space and to send a clean transmission of a selected signal only to that location. In order for the time reversal process to work, a calibration signal must be obtained. This signal may be obtained experimentally using an impulsive sound, a known chirp signal, or other known signals. It may also be determined from a numerical model of a known environment in which the focusing is desired or from passive listening over time to ambient noise.

  18. Differential gene expression profiling of mouse skin after sulfur mustard exposure: Extended time response and inhibitor effect

    SciTech Connect (OSTI)

    Gerecke, Donald R. Chen Minjun; Isukapalli, Sastry S.; Gordon, Marion K.; Chang, Y.-C.; Tong Weida; Androulakis, Ioannis P.; Georgopoulos, Panos G.


    Sulfur mustard (HD, SM), is a chemical warfare agent that within hours causes extensive blistering at the dermal-epidermal junction of skin. To better understand the progression of SM-induced blistering, gene expression profiling for mouse skin was performed after a single high dose of SM exposure. Punch biopsies of mouse ears were collected at both early and late time periods following SM exposure (previous studies only considered early time periods). The biopsies were examined for pathological disturbances and the samples further assayed for gene expression profiling using the Affymetrix microarray analysis system. Principal component analysis and hierarchical cluster analysis of the differently expressed genes, performed with ArrayTrack showed clear separation of the various groups. Pathway analysis employing the KEGG library and Ingenuity Pathway Analysis (IPA) indicated that cytokine-cytokine receptor interaction, cell adhesion molecules (CAMs), and hematopoietic cell lineage are common pathways affected at different time points. Gene ontology analysis identified the most significantly altered biological processes as the immune response, inflammatory response, and chemotaxis; these findings are consistent with other reported results for shorter time periods. Selected genes were chosen for RT-PCR verification and showed correlations in the general trends for the microarrays. Interleukin 1 beta was checked for biological analysis to confirm the presence of protein correlated to the corresponding microarray data. The impact of a matrix metalloproteinase inhibitor, MMP-2/MMP-9 inhibitor I, against SM exposure was assessed. These results can help in understanding the molecular mechanism of SM-induced blistering, as well as to test the efficacy of different inhibitors.

  19. SU-E-T-492: Implementing a Method for Brain Irradiation in Rats Utilizing a Commercially Available Radiosurgery Irradiator

    SciTech Connect (OSTI)

    Cates, J; Drzymala, R


    Purpose: The purpose of the study was to implement a method for accurate rat brain irradiation using the Gamma Knife Perfexion unit. The system needed to be repeatable, efficient, and dosimetrically and spatially accurate. Methods: A platform (“rat holder”) was made such that it is attachable to the Leskell Gamma Knife G Frame. The rat holder utilizes two ear bars contacting bony anatomy and a front tooth bar to secure the rat. The rat holder fits inside of the Leskell localizer box, which utilizes fiducial markers to register with the GammaPlan planning system. This method allows for accurate, repeatable setup.A cylindrical phantom was made so that film can be placed axially in the phantom. We then acquired CT image sets of the rat holder and localizer box with both a rat and the phantom. Three treatment plans were created: a plan on the rat CT dataset, a phantom plan with the same prescription dose as the rat plan, and a phantom plan with the same delivery time as the rat plan. Results: Film analysis from the phantom showed that our setup is spatially accurate and repeatable. It is also dosimetrically accurate, with an difference between predicted and measured dose of 2.9%. Film analysis with prescription dose equal between rat and phantom plans showed a difference of 3.8%, showing that our phantom is a good representation of the rat for dosimetry purposes, allowing for +/- 3mm diameter variation. Film analysis with treatment time equal showed an error of 2.6%, which means we can deliver a prescription dose within 3% accuracy. Conclusion: Our method for irradiation of rat brain has been shown to be repeatable, efficient, and accurate, both dosimetrically and spatially. We can treat a large number of rats efficiently while delivering prescription doses within 3% at millimeter level accuracy.

  20. Technical Note: Immunohistochemical evaluation of mouse brain irradiation targeting accuracy with 3D-printed immobilization device

    SciTech Connect (OSTI)

    Zarghami, Niloufar Jensen, Michael D.; Talluri, Srikanth; Dick, Frederick A.; Foster, Paula J.; Chambers, Ann F.; Wong, Eugene


    Purpose: Small animal immobilization devices facilitate positioning of animals for reproducible imaging and accurate focal radiation therapy. In this study, the authors demonstrate the use of three-dimensional (3D) printing technology to fabricate a custom-designed mouse head restraint. The authors evaluate the accuracy of this device for the purpose of mouse brain irradiation. Methods: A mouse head holder was designed for a microCT couch using CAD software and printed in an acrylic based material. Ten mice received half-brain radiation while positioned in the 3D-printed head holder. Animal placement was achieved using on-board image guidance and computerized asymmetric collimators. To evaluate the precision of beam localization for half-brain irradiation, mice were sacrificed approximately 30 min after treatment and brain sections were stained for γ-H2AX, a marker for DNA breaks. The distance and angle of the γ-H2AX radiation beam border to longitudinal fissure were measured on histological samples. Animals were monitored for any possible trauma from the device. Results: Visualization of the radiation beam on ex vivo brain sections with γ-H2AX immunohistochemical staining showed a sharp radiation field within the tissue. Measurements showed a mean irradiation targeting error of 0.14 ± 0.09 mm (standard deviation). Rotation between the beam axis and mouse head was 1.2° ± 1.0° (standard deviation). The immobilization device was easily adjusted to accommodate different sizes of mice. No signs of trauma to the mice were observed from the use of tooth block and ear bars. Conclusions: The authors designed and built a novel 3D-printed mouse head holder with many desired features for accurate and reproducible radiation targeting. The 3D printing technology was found to be practical and economical for producing a small animal imaging and radiation restraint device and allows for customization for study specific needs.

  1. Canted Undulator Upgrade for GeoSoilEnviroCARS Sector 13 at the Advanced Photon Source

    SciTech Connect (OSTI)

    Sutton, Stephen


    Support for the beamline component of the canted undulator upgrade of Sector 13 (GeoSoilEnviroCARS; managed and operated by the University of Chicago) at the Advanced Photon Source (APS; Argonne National Laboratory) was received from three agencies (equally divided): NASA-SRLIDAP (now LARS), NSF-EAR-IF (ARRA) and DOE-Single Investigator Small Group (SISGR). The associated accelerator components (undulators, canted front end) were provided by the APS using DOE-ARRA funding. The intellectual merit of the research enabled by the upgrade lies in advancing our knowledge of the composition, structure and properties of earth materials; the processes they control; and the processes that produce them. The upgrade will facilitate scientific advances in the following areas: high pressure mineral physics and chemistry, non-crystalline and nano-crystalline materials at high pressure, chemistry of hydrothermal fluids, reactions at mineral-water interfaces, biogeochemistry, oxidation states of magmas, flow dynamics of fluids and solids, and cosmochemistry. The upgrade, allowing the microprobe to operate 100% of the time and the high pressure and surface scattering and spectroscopy instruments to receive beam time increases, will facilitate much more efficient use of the substantial investment in these instruments. The broad scientific community will benefit by the increase in the number of scientists who conduct cutting-edge research at GSECARS. The user program in stations 13ID-C (interface scattering) and 13ID-D (laser heated diamond anvil cell and large volume press) recommenced in June 2012. The operation of the 13ID-E microprobe station began in the Fall 2012 cycle (Oct.-Dec 2012). The upgraded canted beamlines double the amount of undulator beam time at Sector 13 and provide new capabilities including extended operations of the X-ray microprobe down to the sulfur K edge and enhanced brightness at high energy. The availability of the upgraded beamlines will advance the

  2. Double trisomy mosaic (47,XXX/48,XXX,+13) confirmed by FISH and skin fibroblast culture

    SciTech Connect (OSTI)

    Lieber, E.; Grady, V.; Dosik, H.


    A 4 lb 8 oz female was born to a 49-year-old woman (P1200G12) at 40 weeks. The baby had tetralogy of Fallot, polydactyly, microcephaly, low set simple ears, posterior cleft of the soft palate and overlapping flexion deformities of both hands. The eyes were deep set. The clinical impression was trisomy 13. The baby is not doing well and needs a gastrotomy tube for feeding. Sucking is allright but swallowing is impeded. An MRI showed an anomaly of the corpus callosum. The ophthalmological examination showed no abnormalities. A chromosome study on a 2-day peripheral blood sample resulted in poor growth and poor morphology; however, 20 Giemsa-banded cells revealed a 47,XXX karyotype. A second specimen was obtained to search for mosaicism and a blood smear revealed nuclear projections on the neutrophils. FISH analysis using whole chromosome painting probe (Life Technologies) first identified the extra chromosome number 13, the final results showing five of sixty metaphase cells (8.3%) with trisomy 13. Cytogenetic analysis using Giemsa-banding technique revealed four cells in fifty examined (8.0%) with a 48,XXX,+13 karyotype. In order to further evaluate the mosaicism, cytogenetic analysis of a skin fibroblast culture was performed. Twenty one of twenty three cells examined (91.3%) showed the 48,XXX,+13 karyotype. FISH analysis of the skin biopsy revealed eighteen of twenty cells (90.9%) with the trisomy 13. The FISH technique is an important enhancement to routine cytogenetic studies when they do not immediately correlate with clinical impressions.

  3. Fully Automated Simultaneous Integrated Boosted-Intensity Modulated Radiation Therapy Treatment Planning Is Feasible for Head-and-Neck Cancer: A Prospective Clinical Study

    SciTech Connect (OSTI)

    Wu Binbin; McNutt, Todd; Zahurak, Marianna; Simari, Patricio; Pang, Dalong; Taylor, Russell; Sanguineti, Giuseppe


    Purpose: To prospectively determine whether overlap volume histogram (OVH)-driven, automated simultaneous integrated boosted (SIB)-intensity-modulated radiation therapy (IMRT) treatment planning for head-and-neck cancer can be implemented in clinics. Methods and Materials: A prospective study was designed to compare fully automated plans (APs) created by an OVH-driven, automated planning application with clinical plans (CPs) created by dosimetrists in a 3-dose-level (70 Gy, 63 Gy, and 58.1 Gy), head-and-neck SIB-IMRT planning. Because primary organ sparing (cord, brain, brainstem, mandible, and optic nerve/chiasm) always received the highest priority in clinical planning, the study aimed to show the noninferiority of APs with respect to PTV coverage and secondary organ sparing (parotid, brachial plexus, esophagus, larynx, inner ear, and oral mucosa). The sample size was determined a priori by a superiority hypothesis test that had 85% power to detect a 4% dose decrease in secondary organ sparing with a 2-sided alpha level of 0.05. A generalized estimating equation (GEE) regression model was used for statistical comparison. Results: Forty consecutive patients were accrued from July to December 2010. GEE analysis indicated that in APs, overall average dose to the secondary organs was reduced by 1.16 (95% CI = 0.09-2.33) with P=.04, overall average PTV coverage was increased by 0.26% (95% CI = 0.06-0.47) with P=.02 and overall average dose to the primary organs was reduced by 1.14 Gy (95% CI = 0.45-1.8) with P=.004. A physician determined that all APs could be delivered to patients, and APs were clinically superior in 27 of 40 cases. Conclusions: The application can be implemented in clinics as a fast, reliable, and consistent way of generating plans that need only minor adjustments to meet specific clinical needs.

  4. The ePLAS Code for Ignition Studies

    SciTech Connect (OSTI)

    Mason, Rodney J


    Inertial Confinement Fusion (ICF) presents unique opportunities for the extraction of clean energy from Fusion. Intense lasers and particle beams can create and interact with such plasmas, potentially yielding sufficient energy to satisfy all our national needs. However, few models are available to help aid the scientific community in the study and optimization of such interactions. This project enhanced and disseminated the computer code ePLAS for the early understanding and control of Ignition in ICF. ePLAS is a unique simulation code that tracks the transport of laser light to a target, the absorption of that light resulting in the generation and transport of hot electrons, and the heating and flow dynamics of the background plasma. It uses an implicit electromagnetic field-solving method to greatly reduce computing demands, so that useful target interaction studies can often be completed in 15 minutes on a portable 2.1 GHz PC. The code permits the rapid scoping of calculations for the optimization of laser target interactions aimed at fusion. Recent efforts have initiated the use of analytic equations of state (EOS), K-alpha image rendering graphics, allocatable memory for source-free usage, and adaption to the latest Mac and Linux Operating Systems. The speed and utility of ePLAS are unequaled in the ICF simulation community. This project evaluated the effects of its new EOSs on target heating, compared fluid and particle models for the ions, initiated the simultaneous use of both ion models in the code, and studied long time scale 500 ps hot electron deposition for shock ignition. ePLAS has been granted EAR99 export control status, permitting export without a license to most foreign countries. Beta-test versions of ePLAS have been granted to several Universities and Commercial users. The net Project was aimed at achieving early success in the laboratory ignition of thermonuclear targets and the mastery of controlled fusion power for the nation.

  5. Hearing Protection Evaluation for the Combat Arms Earplug at Idaho National Laboratory

    SciTech Connect (OSTI)

    James Lovejoy


    The Idaho National Laboratory (INL) is managed by Battelle Energy Alliance, LLC (BEA) for the Department of Energy. The INL Protective Security Forces (Pro Force) are involved in training exercises that generate impulse noise by small arms fire. Force-on-force (FOF) training exercises that simulate real world scenarios require the Pro Force to engage the opposition force (OPFOR) while maintaining situational awareness through verbal communications. The Combat Arms earplug was studied to determine if it provides adequate hearing protection in accordance with the requirements of MIL-STD-1474C/D. The Combat Arms earplug uses a design that allows continuous noise through a critical orifice while effectively attenuating high-energy impulse noise. The earplug attenuates noise on a non linear scale, as the sound increases the attenuation increases. The INL studied the effectiveness of the Combat Arms earplug with a Bruel & Kjaer (B&K) head and torso simulator used with a selection of small arms to create impulse sound pressures. The Combat Arms earplugs were inserted into the B&K head and torso ears, and small arms were then discharged to generate the impulse noise. The INL analysis of the data indicates that the Combat Arms earplug does provide adequate protection, in accordance with MIL-STD-1474C/D, when used to protect against impulse noise generated by small arms fire using blank ammunition. Impulse noise generated by small arms fire ranged from 135160 dB range unfiltered un-weighted. The Combat Arms earplug attenuated the sound pressure 1025 dB depending on the impulse noise pressure. This assessment is consistent with the results of previously published studies on the Combat Arms earplug (see Section 5, References). Based upon these result, the INL intends to use the Combat Arms earplug for FOF training exercises.

  6. Ectopic bone formation and chondrodysplasia in transgenic mice carrying the rat C3(1)/T{sub AG} fusion gene

    SciTech Connect (OSTI)

    Green, J.E.; Maroulakou, I.G.; Anver, M.


    Transgenic mice expressing the SV40 large T-antigen (T{sup AG}) under the regultory control of the hormone-responsive rat C3(1) prostatein promoter develop unusual bone and cartilage lesions, as well as ectopic bone and cartilage formation. Two lines of transgenic animals have been propagated in which the expression of the transgene in chondrocytes results in a mild to moderate generalized disorganization of cartilage growth which appears to affect multiple tissues, including the trachea, ear pinna and articular cartilage. The epiphyseal plates are also affected with normal architecture of the zones of proliferation and maturation, but marked elongation of the zone of hypertrophy. Immunocytochemistry demonstrates that expression of T{sup AG} is limited to the zone of hypertropny in the epiphyseal plates, suggesting that the chondrocytes become hormone-responsive at this particular stage of differentiation. Normal mineralization and trabecular formation in long bone appears to occur. Ectopic bone and cartilage formation occurs in the foot pads of the fore- and hind- feet over the course of several months. This is preceded by proliferation of sweat gland epithelial cells followed by the appearance of nodules of cartilage and bone. The nodules are closely associated with proliferating epithelium but are not contiguous with bony structures normally found in the feet. The roles of BMP`s, growth factors, oncogenes and hormones in the development of these lesions will be presented. These transgenic animals may provide new insights into hormone-responsiveness of chondrocytes, as well as factors involved in the processes of bone and cartilage differentiation and growth. These transgenic animals may serve as a useful model for human heterotopic bone formation.

  7. ASC-ATDM Performance Portability Requirements for 2015-2019

    SciTech Connect (OSTI)

    Edwards, Harold C.; Trott, Christian Robert


    This report outlines the research, development, and support requirements for the Advanced Simulation and Computing (ASC ) Advanced Technology, Development, and Mitigation (ATDM) Performance Portability (a.k.a., Kokkos) project for 2015 - 2019 . The research and development (R&D) goal for Kokkos (v2) has been to create and demonstrate a thread - parallel programming model a nd standard C++ library - based implementation that enables performance portability across diverse manycore architectures such as multicore CPU, Intel Xeon Phi, and NVIDIA Kepler GPU. This R&D goal has been achieved for algorithms that use data parallel pat terns including parallel - for, parallel - reduce, and parallel - scan. Current R&D is focusing on hierarchical parallel patterns such as a directed acyclic graph (DAG) of asynchronous tasks where each task contain s nested data parallel algorithms. This five y ear plan includes R&D required to f ully and performance portably exploit thread parallelism across current and anticipated next generation platforms (NGP). The Kokkos library is being evaluated by many projects exploring algorithm s and code design for NGP. Some production libraries and applications such as Trilinos and LAMMPS have already committed to Kokkos as their foundation for manycore parallelism an d performance portability. These five year requirements includes support required for current and antic ipated ASC projects to be effective and productive in their use of Kokkos on NGP. The greatest risk to the success of Kokkos and ASC projects relying upon Kokkos is a lack of staffing resources to support Kokkos to the degree needed by these ASC projects. This support includes up - to - date tutorials, documentation, multi - platform (hardware and software stack) testing, minor feature enhancements, thread - scalable algorithm consulting, and managing collaborative R&D.

  8. Improved Dosimetric and Clinical Outcomes With Intensity-Modulated Radiotherapy for Head-and-Neck Cancer of Unknown Primary Origin

    SciTech Connect (OSTI)

    Chen, Allen M.; Li Baoqing; Farwell, D. Gregory; Marsano, Joseph; Vijayakumar, Srinivasan; Purdy, James A.


    Purpose: To compare differences in dosimetric, clinical, and quality-of-life endpoints among a cohort of patients treated by intensity-modulated radiotherapy (IMRT) and conventional radiotherapy (CRT) for head-and-neck cancer of unknown primary origin. Methods and Materials: The medical records of 51 patients treated by radiation therapy for squamous cell carcinoma of the head and neck presenting as cervical lymph node metastasis of occult primary origin were reviewed. Twenty-four patients (47%) were treated using CRT, and 27 (53%) were treated using IMRT. The proportions of patients receiving concurrent chemotherapy were 54% and 63%, respectively. Results: The 2-year estimates of overall survival, local-regional control, and disease-specific survival for the entire patient population were 86%, 89%, and84%, respectively. There were no significant differences in any of these endpoints with respect to radiation therapy technique (p > 0.05 for all). Dosimetric analysis revealed that the use of IMRT resulted in significant improvements with respect to mean dose and V30 to the contralateral (spared) parotid gland. In addition, mean doses to the ipsilateral inner and middle ear structures were significantly reduced with IMRT (p < 0.05 for all). The incidence of severe xerostomia in the late setting was 58% and 11% among patients treated by CRT and IMRT, respectively (p < 0.001). The percentages of patients who were G-tube dependent at 6 months after treatment were 42% and 11%, respectively (p < 0.001). Conclusions: IMRT results in significant improvements in the therapeutic ratio among patients treated by radiation therapy for head-and-neck cancer of unknown primary origin.

  9. Estimation of placental and lactational transfer and tissue distribution of atrazine and its main metabolites in rodent dams, fetuses, and neonates with physiologically based pharmacokinetic modeling

    SciTech Connect (OSTI)

    Lin, Zhoumeng; Fisher, Jeffrey W.; Wang, Ran; Ross, Matthew K.; Filipov, Nikolay M.


    Atrazine (ATR) is a widely used chlorotriazine herbicide, a ubiquitous environmental contaminant, and a potential developmental toxicant. To quantitatively evaluate placental/lactational transfer and fetal/neonatal tissue dosimetry of ATR and its major metabolites, physiologically based pharmacokinetic models were developed for rat dams, fetuses and neonates. These models were calibrated using pharmacokinetic data from rat dams repeatedly exposed (oral gavage; 5 mg/kg) to ATR followed by model evaluation against other available rat data. Model simulations corresponded well to the majority of available experimental data and suggest that: (1) the fetus is exposed to both ATR and its major metabolite didealkylatrazine (DACT) at levels similar to maternal plasma levels, (2) the neonate is exposed mostly to DACT at levels two-thirds lower than maternal plasma or fetal levels, while lactational exposure to ATR is minimal, and (3) gestational carryover of DACT greatly affects its neonatal dosimetry up until mid-lactation. To test the model's cross-species extrapolation capability, a pharmacokinetic study was conducted with pregnant C57BL/6 mice exposed (oral gavage; 5 mg/kg) to ATR from gestational day 12 to 18. By using mouse-specific parameters, the model predictions fitted well with the measured data, including placental ATR/DACT levels. However, fetal concentrations of DACT were overestimated by the model (10-fold). This overestimation suggests that only around 10% of the DACT that reaches the fetus is tissue-bound. These rodent models could be used in fetal/neonatal tissue dosimetry predictions to help design/interpret early life toxicity/pharmacokinetic studies with ATR and as a foundation for scaling to humans. - Highlights: We developed PBPK models for atrazine in rat dams, fetuses, and neonates. We conducted pharmacokinetic (PK) study with atrazine in pregnant mice. Model predictions were in good agreement with experimental rat and mouse PK data. The

  10. In utero exposure to benzene increases embryonic c-Myb and Pim-1 protein levels in CD-1 mice

    SciTech Connect (OSTI)

    Wan, Joanne; Winn, Louise M.


    Benzene is a known human leukemogen, but its role as an in utero leukemogen remains controversial. Epidemiological studies have correlated parental exposure to benzene with an increased incidence of childhood leukemias. We hypothesize that in utero exposure to benzene may cause leukemogenesis by affecting the embryonic c-Myb/Pim-1 signaling pathway and that this is mediated by oxidative stress. To investigate this hypothesis, pregnant CD-1 mice were treated with either 800 mg/kg of benzene or corn oil (i.p.) on days 10 and 11 of gestation and in some cases pretreated with 25 kU/kg of PEG-catalase. Phosphorylated and total embryonic c-Myb and Pim-1 protein levels were assessed using Western blotting and maternal and embryonic oxidative stress were assessed by measuring reduced to oxidized glutathione ratios. Our results show increased oxidative stress at 4 and 24 h after exposure, increased phosphorylated Pim-1 protein levels 4 h after benzene exposure, and increased Pim-1 levels at 24 and 48 h after benzene exposure. Embryonic c-Myb levels were elevated at 24 h after exposure. PEG-catalase pretreatment prevented benzene-mediated increases in embryonic c-Myb and Pim-1 protein levels, and benzene-induced oxidative stress. These results support a role for ROS in c-Myb and Pim-1 alterations after in utero benzene exposure.

  11. Prenatal dichlorodiphenyldichloroethylene (DDE) exposure and child growth during the first year of life

    SciTech Connect (OSTI)

    Garced, Sheyla; Torres-Sanchez, Luisa; Cebrian, Mariano E.; Claudio, Luz; Lopez-Carrillo, Lizbeth


    Background: Due to its long-term persistence in the environment and its ability to cross the placental barrier, prenatal p,p Prime -dichlorodiphenyldichloroethene (DDE) exposure continues to be a public health concern. This study aimed to evaluate the association between prenatal DDE exposure and child growth, at birth and during the first year of life. Methods: 253 pregnant women were recruited between January 2001 and June 2005 in a prospective cohort in Morelos, Mexico. Serum levels of DDE were measured during each trimester of pregnancy by gas chromatography with an electron capture detector. Using the generalized mixed-effects models, the association between DDE and child growth parameters (weight-for-age, length-for-age, weight-for-length, BMI-for-age and head circumference-for-age Z-scores) from birth to 1 year of age was assessed. Maternal dietary intake was considered as covariable among others. Results: DDE levels were 6.3{+-}2.8 ng/mL (first trimester), 6.6{+-}2.9 ng/mL (second trimester), and 7.6{+-}2.9 ng/mL (third trimester). After adjusting for potential confounder variables, no significant associations were observed with prenatal DDE exposure and each of the selected parameters. Conclusions: Our results show no evidence of an association between prenatal DDE exposure and child growth during the first year of life.

  12. Genome structure and emerging evidence of an incipient sex chromosome in Populus

    SciTech Connect (OSTI)

    Yin, Tongming; DiFazio, Stephen P; Gunter, Lee E; Zhang, Xinye; Sewell, Mitchell; Woolbright, Dr. Scott; Allan, Dr. Gery; Kelleher, Colin; Douglas, Carl; Wang, Prof. Mingxiu; Tuskan, Gerald A


    The genus Populus consists of dioecious woody species with largely unknown genetic mechanisms for gender determination. We have discovered genetic and genomic features in the peritelomeric region of chromosome XIX that suggest this region of the Populus genome is in the process of developing characteristics of a sex chromosome. We have identified a gender-associated locus that consistently maps to this region. Furthermore, comparison of genetic maps across multiple Populus families reveals consistently distorted segregation within this region. We have intensively characterized this region using an F1 interspecific cross involving the female genotype that was used for genome sequencing. This region shows suppressed recombination and high divergence between the alternate haplotypes, as revealed by dense map-based genome assembly using microsatellite markers. The suppressed recombination, distorted segregation, and haplotype divergence were observed only for the maternal parent in this cross. Furthermore, the progeny of this cross showed a strongly male-biased sex ratio, in agreement with Haldane's rule that postulates that the heterogametic sex is more likely to be absent, rare, or sterile in interspecific crosses. Together, these results support the role of chromosome XIX in sex determination and suggest that sex determination in Populus occurs through a ZW system in which the female is the heterogametic gender.

  13. Asymptomatic and late-onset ornithine transcarbamylase deficiency caused by a A208T mutation: Clinical, biochemical and DNA analyses in a four-generation family

    SciTech Connect (OSTI)

    Ausems, M.G.E.M.; Bakker, E.; Kneppers, A.L.J.


    We describe a 4-generation family in which a previously healthy 10-year-old boy died of late-onset ornithine transcarbamylase (OTC) deficiency. Pedigree analysis and allopurinol loading tests in female relatives were not informative. A missense mutation (A208T) in the OTC gene was detected in the deceased patient and in several clinically healthy male and female relatives, the oldest male being 97 years old. OTC deficiency was established in autopsy liver tissue of the propositus and liver biopsy samples of his sister, mother, and a maternal uncle. The males had 4% and 6% residual activity, respectively, the females 58% and 67%, respectively. The observed relation between the mutation and the decreased OTC activity in liver tissue of these subjects suggests that the mutation is a deleterious one. Late-onset, {open_quotes}mild{close_quotes} OTC deficiency can have a fatal or a favorable outcome. The disease can segregate undetected in families. 16 refs., 1 fig., 1 tab.

  14. Methods And Compositions For Chromosome-Specific Staining

    DOE Patents [OSTI]

    Gray, Joe W.; Pinkel, Daniel


    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods are provided to disable the hybridization capacity of shared, high copy repetitive sequences and/or remove such sequences to provide for useful contrast. Still further methods are provided to produce chromosome-specific staining reagents which are made specific to the targeted chromosomal material, which can be one or more whole chromosomes, one or more regions on one or more chromosomes, subsets of chromosomes and/or the entire genome. Probes and test kits are provided for use in tumor cytogenetics, in the detection of disease related loci, in analysis of structural abnormalities, such as translocations, and for biological dosimetry. Further, methods and prenatal test kits are provided to stain targeted chromosomal material of fetal cells, including fetal cells obtained from maternal blood. Still further, the invention provides for automated means to detect and analyse chromosomal abnormalities.

  15. Inhalation developmental toxicology studies: Gallium arsenide in mice and rats

    SciTech Connect (OSTI)

    Mast, T.J.; Greenspan, B.J.; Dill, J.A.; Stoney, K.H.; Evanoff, J.J.; Rommereim, R.L.


    Gallium arsenide is a crystalline compound used extensively in the semiconductor industry. Workers preparing solar cells and gallium arsenide ingots and wafers are potentially at risk from the inhalation of gallium arsenide dust. The potential for gallium arsenide to cause developmental toxicity was assessed in Sprague- Dawley rats and CD-1 (Swiss) mice exposed to 0, 10, 37, or 75 mg/m{sup 3} gallium arsenide, 6 h/day, 7 days/week. Each of the four treatment groups consisted of 10 virgin females (for comparison), and {approx}30 positively mated rats or {approx}24 positively mated mice. Mice were exposed on 4--17 days of gestation (dg), and rats on 4--19 dg. The day of plug or sperm detection was designated as 0 dg. Body weights were obtained throughout the study period, and uterine and fetal body weights were obtained at sacrifice (rats, 20 dg; mice, 18 dg). Implants were enumerated and their status recorded. Live fetuses were sexed and examined for gross, visceral, skeletal, and soft-tissue craniofacial defects. Gallium and arsenic concentrations were determined in the maternal blood and uterine contents of the rats (3/group) at 7, 14, and 20 dg. 37 refs., 11 figs., 30 tabs.

  16. Biomarkers of Radiosensitivity in A-Bomb Survivors Pregnant at the Time of Bombings in Hiroshima and Nagasaki


    Miles, Edward F.; Tatsukawa, Yoshimi; Funamoto, Sachiyo; Kamada, Naoko; Nakashima, Eiji; Kodama, Yoshiaki; Seed, Thomas; Kusonoki, Yoichiro; Nakachi, Kei; Fujiwara, Saeko; et al


    Purpose . There is evidence in the literature of increased maternal radiosensitivity during pregnancy. Materials and Methods . We tested this hypothesis using information from the atomic-bomb survivor cohort, that is, the Adult Health Study database at the Radiation Effects Research Foundation, which contains data from a cohort of women who were pregnant at the time of the bombings of Hiroshima and Nagasaki. Previous evaluation has demonstrated long-term radiation dose-response effects. Results/Conclusions . Data on approximately 250 women were available to assess dose-response rates for serum cholesterol, white blood cell count, erythrocyte sedimentation rate, and serum hemoglobin, and onmore » approximately 85 women for stable chromosome aberrations, glycophorin A locus mutations, and naïve CD4 T-cell counts. Although there is no statistically significant evidence of increased radiosensitivity in pregnant women, the increased slope of the linear trend line in the third trimester with respect to stable chromosome aberrations is suggestive of an increased radiosensitivity.« less

  17. Somatic genomic variations in extra-embryonic tissues

    SciTech Connect (OSTI)

    Weier, Jingly F.; Ferlatte, Christy; Weier, Heinz-Ulli G.


    In the mature chorion, one of the membranes that exist during pregnancy between the developing fetus and mother, human placental cells form highly specialized tissues composed of mesenchyme and floating or anchoring villi. Using fluorescence in situ hybridization, we found that human invasive cytotrophoblasts isolated from anchoring villi or the uterine wall had gained individual chromosomes; however, chromosome losses were detected infrequently. With chromosomes gained in what appeared to be a chromosome-specific manner, more than half of the invasive cytotrophoblasts in normal pregnancies were found to be hyperdiploid. Interestingly, the rates of hyperdiploid cells depended not only on gestational age, but were strongly associated with the extraembryonic compartment at the fetal-maternal interface from which they were isolated. Since hyperdiploid cells showed drastically reduced DNA replication as measured by bromodeoxyuridine incorporation, we conclude that aneuploidy is a part of the normal process of placentation potentially limiting the proliferative capabilities of invasive cytotrophoblasts. Thus, under the special circumstances of human reproduction, somatic genomic variations may exert a beneficial, anti-neoplastic effect on the organism.

  18. Methods of biological dosimetry employing chromosome-specific staining

    DOE Patents [OSTI]

    Gray, Joe W.; Pinkel, Daniel


    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods are provided to disable the hybridization capacity of shared, high copy repetitive sequences and/or remove such sequences to provide for useful contrast. Still further methods are provided to produce chromosome-specific staining reagents which are made specific to the targeted chromosomal material, which can be one or more whole chromosomes, one or more regions on one or more chromosomes, subsets of chromosomes and/or the entire genome. Probes and test kits are provided for use in tumor cytogenetics, in the detection of disease related loci, in analysis of structural abnormalities, such as translocations, and for biological dosimetry. Further, methods and prenatal test kits are provided to stain targeted chromosomal material of fetal cells, including fetal cells obtained from maternal blood. Still further, the invention provides for automated means to detect and analyse chromosomal abnormalities.

  19. Compositions for chromosome-specific staining

    DOE Patents [OSTI]

    Gray, J.W.; Pinkel, D.


    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. The methods produce staining patterns that can be tailored for specific cytogenetic analyses. The probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods are provided to disable the hybridization capacity of shared, high copy repetitive sequences and/or remove such sequences to provide for useful contrast. Still further methods are provided to produce chromosome-specific staining reagents which are made specific to the targeted chromosomal material, which can be one or more whole chromosomes, one or more regions on one or more chromosomes, subsets of chromosomes and/or the entire genome. Probes and test kits are provided for use in tumor cytogenetics, in the detection of disease related loci, in analysis of structural abnormalities, such as translocations, and for biological dosimetry. Methods and prenatal test kits are provided to stain targeted chromosomal material of fetal cells, including fetal cells obtained from maternal blood. The invention provides for automated means to detect and analyze chromosomal abnormalities. 17 figs.

  20. Compositions for chromosome-specific staining

    DOE Patents [OSTI]

    Gray, Joe W.; Pinkel, Daniel


    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods are provided to disable the hybridization capacity of shared, high copy repetitive sequences and/or remove such sequences to provide for useful contrast. Still further methods are provided to produce chromosome-specific staining reagents which are made specific to the targeted chromosomal material, which can be one or more whole chromosomes, one or more regions on one or more chromosomes, subsets of chromosomes and/or the entire genome. Probes and test kits are provided for use in tumor cytogenetics, in the detection of disease related loci, in analysis of structural abnormalities, such as translocations, and for biological dosimetry. Further, methods and prenatal test kits are provided to stain targeted chromosomal material of fetal cells, including fetal cells obtained from maternal blood. Still further, the invention provides for automated means to detect and analyse chromosomal abnormalities.

  1. Genetic Analysis of Snake River Sockeye Salmon (Oncorhynchus Nerka), 2003 Technical Report.

    SciTech Connect (OSTI)

    Faler, Joyce; Powell, Madison


    A total of 1720 Oncorhynchus nerka tissue samples from 40 populations were characterized using mitochondrial DNA RFLPs (Restriction Fragment Length Polymorphisms). Analysis of anadromous sockeye populations indicated the historical presence of four major maternal lineages. Thirty-five composite mitochondrial haplotypes were observed from the 40 populations of O. nerka sampled throughout the Pacific Northwest. Six of these composite haplotypes ranged in frequency from 7-26% overall and were commonly observed in most populations. The six haplotypes together comprised 90% of the sampled O. nerka. An average of 4.6 composite haplotypes were observed per population. Genetic markers used were satisfactory in separating Redfish Lake anadromous sockeye, residual sockeye and outmigrants from the sympatric kokanee population that spawns in the Fishhook Creek tributary. Outmigrants appear to be primarily composed of progeny from resident residual sockeye, and captively-reared progeny of the captive broodstock program. Thus, residual sockeye may be considered a suitable source of genetic variation to maintain genetic diversity among captive broodstocks of anadromous sockeye. Fishhook Creek kokanee are genetically diverse and during spawning, are temporally and spatially isolated from the residual sockeye population. Eleven composite haplotypes were observed in the kokanee population. The unusually high number of haplotypes is most likely a consequence of periodic stocking of Redfish Lake with kokanee from other sources. Genetic data from Redfish Lake creel samples taken during 1996-1999 putatively indicate the incidental take of a listed resident sockeye.

  2. Molecular epidemiology of childhood leukemia with emphasis on chemical exposures

    SciTech Connect (OSTI)

    Buffler, P.A.; Smith, M.T.; Wood, S.; Reynolds, P.


    Developing markets in the Pacific Basin depend heavily on the production and export of consumer goods. The generation of hazardous waste as a by-product of industrial production can be linked to adverse health outcomes, such as childhood leukemia, in ways that are presently unknown. In California, exposures resulting from hazardous waste disposal are of concern in the etiology of childhood cancer. Approximately 63% of the 57 hazardous waste sites that the U.S. Environmental Protection Agency (USEPA) included in the national priority list under the Comprehensive Environmental Response, Compensation and Liability Act (CERCLA) statute were in the six-county San Francisco Bay area. This area includes California`s Silicon Valley, where a disproportionate majority of these sites are located. Although only one study links hazardous waste disposal to childhood leukemia evidence is accumulating that in utero and maternal pesticide exposures as well as chemical exposures during childhood are important in the etiology of childhood leukemia. This study investigates whether children with leukemia have common genetic changes, whether children with genetic changes experience common chemical exposures, and whether the occurrences of these genetic changes correspond to the same temporal sequence as exposure. The purpose of this paper is to describe the study design and report on the status of research activity. 10 refs., 1 fig., 3 tabs.


    SciTech Connect (OSTI)

    Salaymeh, S.; Jeffcoat, R.


    Sonar and speech techniques have been investigated to improve functionality and enable handheld and other man-portable, mobile, and portal systems to positively detect and identify illicit nuclear materials, with minimal data and with minimal false positives and false negatives. RadSonar isotope detection and identification is an algorithm development project funded by NA-22 and employing the resources of Savannah River National Laboratory and three University Laboratories (JHU-APL, UT-ARL, and UW-APL). Algorithms have been developed that improve the probability of detection and decrease the number of false positives and negatives. Two algorithms have been developed and tested. The first algorithm uses support vector machine (SVM) classifiers to determine the most prevalent nuclide(s) in a spectrum. It then uses a constrained weighted least squares fit to estimate and remove the contribution of these nuclide(s) to the spectrum, iterating classification and fitting until there is nothing of significance left. If any Special Nuclear Materials (SNMs) were detected in this process, a second tier of more stringent classifiers are used to make the final SNM alert decision. The second algorithm is looking at identifying existing feature sets that would be relevant in the radioisotope identification context. The underlying philosophy here is to identify parallels between the physics and/or the structures present in the data for the two applications (speech analysis and gamma spectroscopy). The expectation is that similar approaches may work in both cases. The mel-frequency cepstral representation of spectra is widely used in speech, particularly for two reasons: approximation of the response of the human ear, and simplicity of channel effect separation (in this context, a 'channel' is a method of signal transport that affects the signal, examples being vocal tract shape, room echoes, and microphone response). Measured and simulated gamma-ray spectra from a hand

  4. Development of Biomarkers for Chronic Beryllium Disease in Mice

    SciTech Connect (OSTI)

    Gordon, Terry


    Beryllium is a strategic metal, indispensable for national defense programs in aerospace, telecommunications, electronics, and weaponry. Exposure to beryllium is an extensively documented occupational hazard that causes irreversible, debilitating granulomatous lung disease in as much as 3 - 5% of exposed workers. Mechanistic research on beryllium exposure-disease relationships has been severely limited by a general lack of a sufficient CBD animal model. We have now developed and tested an animal model which can be used for dissecting dose-response relationships and pathogenic mechanisms and for testing new diagnostic and treatment paradigms. We have created 3 strains of transgenic mice in which the human antigen-presenting moiety, HLA-DP, was inserted into the mouse genome. Each mouse strain contains HLA-DPB1 alleles that confer different magnitude of risk for chronic beryllium disease (CBD): HLA-DPB1*0401 (odds ratio = 0.2), HLA-DPB1*0201 (odds ratio = 15), HLA-DPB1*1701 (odds ratio = 240). Our preliminary work has demonstrated that the *1701 allele, as predicted by human studies, results in the greatest degree of sensitization in a mouse ear swelling test. We have also completed dose-response experiments examining beryllium-induced lung granulomas and identified susceptible and resistant inbred strains of mice (without the human transgenes) as well as quantitative trait loci that may contain gene(s) that modify the immune response to beryllium. In this grant application, we propose to use the transgenic and normal inbred strains of mice to identify biomarkers for the progression of beryllium sensitization and CBD. To achieve this goal, we propose to compare the sensitivity and accuracy of the lymphocyte proliferation test (blood and bronchoalveolar lavage fluid) with the ELISPOT test in the three HLA-DP transgenic mice strains throughout a 6 month treatment with beryllium particles. Because of the availability of high-throughput proteomics, we will also identify

  5. Response-surface models for deterministic effects of localized irradiation of the skin by discrete {beta}/{gamma} -emitting sources

    SciTech Connect (OSTI)

    Scott, B.R.


    Individuals who work at nuclear reactor facilities can be at risk for deterministic effects in the skin from exposure to discrete {Beta}- and {gamma}-emitting ({Beta}{gamma}E) sources (e.g., {Beta}{gamma}E hot particles) on the skin or clothing. Deterministic effects are non-cancer effects that have a threshold and increase in severity as dose increases (e.g., ulcer in skin). Hot {Beta}{gamma}E particles are {sup 60}Co- or nuclear fuel-derived particles with diameters > 10 {mu}m and < 3 mm and contain at least 3.7 kBq (0.1 {mu}Ci) of radioactivity. For such {Beta}{gamma}E sources on the skin, it is the beta component of the dose that is most important. To develop exposure limitation systems that adequately control exposure of workers to discrete {Beta}{gamma}E sources, models are needed for systems that adequately control exposure of workers to discrete {Beta}{gamma}E sources, models are needed for evaluating the risk of deterministic effects of localized {Beta} irradiation of the skin. The purpose of this study was to develop dose-rate and irradiated-area dependent, response-surface models for evaluating risks of significant deterministic effects of localized irradiation of the skin by discrete {Beta}{gamma}E sources and to use modeling results to recommend approaches to limiting occupational exposure to such sources. The significance of the research results as follows: (1) response-surface models are now available for evaluating the risk of specific deterministic effects of localized irradiation of the skin; (2) modeling results have been used to recommend approaches to limiting occupational exposure of workers to {Beta} radiation from {Beta}{gamma}E sources on the skin or on clothing; and (3) the generic irradiated-volume, weighting-factor approach to limiting exposure can be applied to other organs including the eye, the ear, and organs of the respiratory or gastrointestinal tract and can be used for both deterministic and stochastic effects.

  6. Ultrasonic imaging of materials under unconventional circumstances

    SciTech Connect (OSTI)

    Declercq, Nico Felicien McKeon, Peter Liu, Jingfei; Shaw, Anurupa; Slah, Yaacoubi


    This paper reflects the contents of the plenary talk given by Nico Felicien Declercq. “Ultrasonic Imaging of materials” covers a wide technological area with main purpose to look at and to peek inside materials. In an ideal world one would manage to examine materials like a clairvoyant. Fortunately this is impossible hence nature has offered sufficient challenges to mankind to provoke curiosity and to develop science and technology. Here we focus on the appearance of certain undesired physical effects that prohibit direct imaging of materials in ultrasonic C-scans. Furthermore we try to make use of these effects to obtain indirect images of materials and therefore make a virtue of necessity. First we return to one of the oldest quests in the progress of mankind: how thick is ice? Our ancestors must have faced this question early on during migration to Northern Europe and to the America’s and Asia. If a physicist or engineer is not provided with helpful tools such as a drill or a device based on ultrasound, it is difficult to determine the ice thickness. Guided waves, similar to those used for nondestructive testing of thin plates in structural health monitoring can be used in combination with the human ear to determine the thickness of ice. To continue with plates, if an image of its interior is desired high frequency ultrasonic pulses can be applied. It is known by the physicist that the resolution depends on the wavelength and that high frequencies usually result in undesirably high damping effects inhibiting deep penetration into the material. To the more practical oriented engineer it is known that it is advantageous to polish surfaces before examination because scattering and diffraction of sound lowers the image resolution. Random scatterers cause some blurriness but cooperating scatters, causing coherent diffraction effects similar to the effects that cause DVD’s to show rainbow patterns under sunlight, can cause spooky images and erroneous

  7. ePLAS Development for Jet Modeling and Applications

    SciTech Connect (OSTI)

    Dr. Rodney J. Mason


    Plasma jets provide an alternate approach to the creation of high energy density laboratory plasmas (HEDLP). For the Plasma Liner Experiment (PLX), typically 30 partially ionized argon jets, produced with mini-rail guns, will be focused into a central volume for subsequent magnetic compression into high density plasma liners that can reach high (0.1 Mbar) peak pressures upon stagnation. The jets are typically 2.5 cm in radius traveling at Mach number 30. Ultimate success will require optimized tuning of the rail configurations, the nozzles injecting the gases, and the careful implementation of pre-ionization. The modeling of plasma jet transport is particularly challenging, due the large space (100 sq cm) and time scales (microseconds) involved. Even traditional implicit methods are insufficient, due to the usual need to track electrons explicitly on the mesh. Wall emission and chemistry must be managed, as must ionization of the jet plasma. Ions in the jets are best followed as particles to account properly for collisions upon jet merger. This Phase I Project developed the code ePLAS to attack and successfully surmount many of these challenges. It invented a new 'super implicit' electromagnetic scheme, using implicit electron moment currents that allowed for modeling of jets over multi-cm and multi-picoseconds on standard, single processor 2 GHz PCs. It enabled merger studies of two jets, in preparation for the multi-jet merger problem. The Project explored particle modeling for the ions, and prepared for the future addition of a grid-base jet ion collision model. Access was added to tabular equations of state for the study of ionization effects in merging jets. The improved code was discussed at the primary plasma meetings (IEEE and APS) during the Project period. Collaborations with National Laboratory and industrial partners were nurtured. Code improvements were made to facilitate code use. See: The ePLAS code enjoys EAR

  8. Effects of Cell Phone Radiofrequency Signal Exposure on Brain Glucos Metabolism

    SciTech Connect (OSTI)

    Volkow, N.D.; Wang, G.; Volkow, N.D.; Tomasi, D.; Wang, G.-J.; Vaska, P.; Fowler, J.S.; Telang, F.; Alexoff, D.; Logan, J.; Wong, C.


    The dramatic increase in use of cellular telephones has generated concern about possible negative effects of radiofrequency signals delivered to the brain. However, whether acute cell phone exposure affects the human brain is unclear. To evaluate if acute cell phone exposure affects brain glucose metabolism, a marker of brain activity. Randomized crossover study conducted between January 1 and December 31, 2009, at a single US laboratory among 47 healthy participants recruited from the community. Cell phones were placed on the left and right ears and positron emission tomography with ({sup 18}F)fluorodeoxyglucose injection was used to measure brain glucose metabolism twice, once with the right cell phone activated (sound muted) for 50 minutes ('on' condition) and once with both cell phones deactivated ('off' condition). Statistical parametric mapping was used to compare metabolism between on and off conditions using paired t tests, and Pearson linear correlations were used to verify the association of metabolism and estimated amplitude of radiofrequency-modulated electromagnetic waves emitted by the cell phone. Clusters with at least 1000 voxels (volume >8 cm{sup 3}) and P < .05 (corrected for multiple comparisons) were considered significant. Brain glucose metabolism computed as absolute metabolism ({micro}mol/100 g per minute) and as normalized metabolism (region/whole brain). Whole-brain metabolism did not differ between on and off conditions. In contrast, metabolism in the region closest to the antenna (orbitofrontal cortex and temporal pole) was significantly higher for on than off conditions (35.7 vs 33.3 {micro}mol/100 g per minute; mean difference, 2.4 [95% confidence interval, 0.67-4.2]; P = .004). The increases were significantly correlated with the estimated electromagnetic field amplitudes both for absolute metabolism (R = 0.95, P < .001) and normalized metabolism (R = 0.89; P < .001). In healthy participants and compared with no exposure, 50-minute

  9. Institute for Scientific Computing Research Fiscal Year 2002 Annual Report

    SciTech Connect (OSTI)

    Keyes, D E; McGraw, J R; Bodtker, L K


    The Institute for Scientific Computing Research (ISCR) at Lawrence Livermore National Laboratory is jointly administered by the Computing Applications and Research Department (CAR) and the University Relations Program (URP), and this joint relationship expresses its mission. An extensively externally networked ISCR cost-effectively expands the level and scope of national computational science expertise available to the Laboratory through CAR. The URP, with its infrastructure for managing six institutes and numerous educational programs at LLNL, assumes much of the logistical burden that is unavoidable in bridging the Laboratory's internal computational research environment with that of the academic community. As large-scale simulations on the parallel platforms of DOE's Advanced Simulation and Computing (ASCI) become increasingly important to the overall mission of LLNL, the role of the ISCR expands in importance, accordingly. Relying primarily on non-permanent staffing, the ISCR complements Laboratory research in areas of the computer and information sciences that are needed at the frontier of Laboratory missions. The ISCR strives to be the ''eyes and ears'' of the Laboratory in the computer and information sciences, in keeping the Laboratory aware of and connected to important external advances. It also attempts to be ''feet and hands, in carrying those advances into the Laboratory and incorporating them into practice. In addition to conducting research, the ISCR provides continuing education opportunities to Laboratory personnel, in the form of on-site workshops taught by experts on novel software or hardware technologies. The ISCR also seeks to influence the research community external to the Laboratory to pursue Laboratory-related interests and to train the workforce that will be required by the Laboratory. Part of the performance of this function is interpreting to the external community appropriate (unclassified) aspects of the Laboratory's own contributions

  10. Developmental exposure to a commercial PBDE mixture, DE-71: neurobehavioral, hormonal, and reproductive effects.

    SciTech Connect (OSTI)

    Kodavanti, Prasada; Coburn, Cary; Moser, Virginia; MacPhail, Robert; Fenton, Suzanne; Stoker, Tammy; Birnbaum, Linda


    Developmental effects of polybrominated diphenyl ethers (PBDEs) have been suspected due to their structural similarities to polychlorinated biphenyls (PCBs). This study evaluated neurobehavioral, hormonal, and reproductive effects in rat offspring perinatally exposed to a widely used pentabrominated commercial mixture, DE-71. Pregnant Long-Evans rats were exposed to 0, 1.7, 10.2, or 30.6 mg/kg/day DE-71 in corn oil by oral gavage from gestational day 6 to weaning. DE-71 did not alter maternal or male offspring body weights. However, female offspring were smaller compared with controls from postnatal days (PNDs) 35-60. Although several neurobehavioral endpoints were assessed, the only statistically significant behavioral finding was a dose-by-age interaction in the number of rears in an open-field test. Developmental exposure to DE-71 caused severe hypothyroxinemia in the dams and early postnatal offspring. DE-71 also affected anogenital distance and preputial separation in male pups. Body weight gain over time, reproductive tissue weights, and serum testosterone concentrations at PND 60 were not altered. Mammary gland development of female offspring was significantly affected at PND 21. Congener-specific analysis of PBDEs indicated accumulation in all tissues examined. Highest PBDE concentrations were found in fat including milk, whereas blood had the lowest concentrations on a wet weight basis. PBDE concentrations were comparable among various brain regions. Thus, perinatal exposure to DE-71 leads to accumulation of PBDE congeners in various tissues crossing blood-placenta and blood-brain barriers, causing subtle changes in some parameters of neurobehavior and dramatic changes in circulating thyroid hormone levels, as well as changes in both male and female reproductive endpoints. Some of these effects are similar to those seen with PCBs, and the persistence of these changes requires further investigation.

  11. The potential reproductive, neurobehavioral and systemic effects of soluble sodium tungstate exposure in Sprague-Dawley rats

    SciTech Connect (OSTI)

    McInturf, S.M. [Naval Medical Research Unit at Dayton (NAMRU), Wright Patterson Air Force Base, OH (United States); Bekkedal, M.Y.V. [Two Steps Forward, LLC, Sun Prairie, WI (United States); Wilfong, E. [U.S. Naval Academy, 572M Holloway Road, Annapolis, MD (United States); Arfsten, D. [Navy Drug Screening Laboratory P.O. Box 113, Naval Air Station Jacksonville, FL (United States); Chapman, G. [Naval Medical Research Unit at Dayton (NAMRU), Wright Patterson Air Force Base, OH (United States); Gunasekar, P.G., E-mail: [Naval Medical Research Unit at Dayton (NAMRU), Wright Patterson Air Force Base, OH (United States)


    The debate on tungsten (W) is fostered by its continuous usage in military munitions. Reports demonstrate W solubilizes in soil and can migrate into drinking water supplies and, therefore, is a potential health risk to humans. This study evaluated the reproductive, systemic and neurobehavioral effects of sodium tungstate (NaW) in rats following 70 days of daily pre-and postnatal exposure via oral gavage to 5, 62.5 and 125 mg/kg/day of NaW through mating, gestation and weaning (PND 0-20). Daily administration of NaW produced no overt evidence of toxicity and had no apparent effect on mating success or offspring physical development. Distress vocalizations were elevated in F{sub 1} offspring from the high dose group, whereas righting reflex showed unexpected sex differences where males demonstrated faster righting than females; however, the effects were not dose-dependent. Locomotor activity was affected in both low and high-dose groups of F{sub 1} females. Low-dose group showed increased distance traveled, more time in ambulatory movements and less time in stereotypic behavior than controls or high dose animals. The high-dose group had more time in stereotypical movements than controls, and less time resting than controls and the lowest exposure group. Maternal retrieval was not affected by NaW exposure. Tungsten analysis showed a systemic distribution of NaW in both parents and offspring, with preferential uptake within the immune organs, including the femur, spleen and thymus. Histopathological evidence suggested no severe chronic injury or loss of function in these organs. However, the heart showed histological lesions, histiocytic inflammation from minimal to mild with cardiomyocyte degeneration and necrosis in several P{sub 0} animals of 125 mg NaW dose group. The result of this study suggests that pre and postnatal exposure to NaW may produce subtle neurobehavioral effects in offspring related to motor activity and emotionality.

  12. Lead, mercury, and cadmium exposure and attention deficit hyperactivity disorder in children

    SciTech Connect (OSTI)

    Kim, Stephani [Division of Epidemiology and Biostatistics, Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, OH 45267 (United States)] [Division of Epidemiology and Biostatistics, Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, OH 45267 (United States); Arora, Monica [Department of Psychiatry, Creighton University School of Medicine, Omaha, NE 68131 (United States)] [Department of Psychiatry, Creighton University School of Medicine, Omaha, NE 68131 (United States); Fernandez, Cristina [Department of Pediatrics, Creighton University School of Medicine, Omaha, NE 68131 (United States)] [Department of Pediatrics, Creighton University School of Medicine, Omaha, NE 68131 (United States); Landero, Julio; Caruso, Joseph [Metallomics Center, Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221 (United States)] [Metallomics Center, Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221 (United States); Chen, Aimin, E-mail: [Division of Epidemiology and Biostatistics, Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, OH 45267 (United States)] [Division of Epidemiology and Biostatistics, Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, OH 45267 (United States)


    Background: There is limited research examining the relationship between lead (Pb) exposure and medically diagnosed attention deficit hyperactivity disorder (ADHD) in children. The role of mercury (Hg) and cadmium (Cd) exposures in ADHD development is even less clear. Objectives: To examine the relationship between Pb, Hg, and Cd and ADHD in children living inside and outside a Lead Investigation Area (LIA) of a former lead refinery in Omaha, NE. Methods: We carried out a case-control study with 71 currently medically diagnosed ADHD cases and 58 controls from a psychiatric clinic and a pediatric clinic inside and outside of the LIA. The participants were matched on age group (58, 912 years), sex, race (African American or Caucasians and others), and location (inside or outside LIA). We measured whole blood Pb, total Hg, and Cd using inductively coupled plasma mass spectrometry. Results: Inside the LIA, the 27 cases had blood Pb geometric mean (GM) 1.89 g/dL and the 41 controls had 1.51 g/dL. Outside the LIA, the 44 cases had blood Pb GM 1.02 g/dL while the 17 controls had 0.97 g/dL. After adjustment for matching variables and maternal smoking, socioeconomic status, and environmental tobacco exposure, each natural log unit blood Pb had an odds ratio of 2.52 with 95% confidence interval of 1.075.92. Stratification by the LIA indicated similar point estimate but wider CIs. No associations were observed for Hg or Cd. Conclusions: Postnatal Pb exposure may be associated with higher risk of clinical ADHD, but not the postnatal exposure to Hg or Cd. -- Highlights: Blood Pb levels are associated with ADHD diagnosis in children. No association was found between blood Cd or Hg levels and ADHD. Children living close to hazardous waste site need to reduce metal exposure.

  13. Sex ratios in fetuses and liveborn infants with autosomal aneuploidy

    SciTech Connect (OSTI)

    Heuther, C.A.; Martin, R.L.M.; Stoppelman, S.M. [Univ. of Cincinnati, OH (United States)] [and others


    Ten data sources were used substantially to increase the available data for estimating fetal and livebirth sex ratios for Patau (trisomy 13), Edwards (trisomy 18), and Down (trisomy 21) syndromes and controls. The fetal sex ratio estimate was 0.88 (N = 584) for trisomy 13, 0.90 (N = 1702) for trisomy 18, and 1.16 (N = 3154) for trisomy 21. All were significantly different from prenatal controls (1.07). The estimated ratios in prenatal controls were 1.28 (N = 1409) for CVSs and 1.06 (N = 49427) for amniocenteses, indicating a clear differential selection against males, mostly during the first half of fetal development. By contrast, there were no sex ratio differences for any of the trisomies when comparing gestational ages <16 and >16 weeks. The livebirth sex ratio estimate was 0.90 (N = 293) for trisomy 13, 0.63 (N = 497) for trisomy 18, and 1.15 (N = 6424) for trisomy 21, the latter two being statistically different than controls (1.05) (N = 3660707). These ratios for trisomies 13 and 18 were also statistically different than the ratio for trisomy 21. Only in trisomy 18 did the sex ratios in fetuses and livebirths differ, indicating a prenatal selection against males >16 weeks. No effects of maternal age or race were found on these estimates for any of the fetal or livebirth trisomies. Sex ratios for translocations and mosaics were also estimated for these aneuploids. Compared to previous estimates, these results are less extreme, most likely because of larger sample sizes and less sample bias. They support the hypothesis that these trisomy sex ratios are skewed at conception, or become so during embryonic development through differential intrauterine selection. The estimate for Down syndrome livebirths is also consistent with the hypothesis that its higher sex ratio is associated with paternal nondisjunction. 36 refs., 5 tabs.

  14. Early life ethanol exposure causes long-lasting disturbances in rat mesenchymal stem cells via epigenetic modifications

    SciTech Connect (OSTI)

    Leu, Yu-Wei; Chu, Pei-Yi; Chen, Chien-Min; Yeh, Kun-Tu; Liu, Yu Ming; Lee, Yen-Hui; Kuo, Shan-Tsu; Hsiao, Shu-Huei


    Highlights: • Ethanol exposure alters proliferation and differentiation of MSCs. • Ethanol exposure suppresses osteogenesis and adipogenesis of MSCs. • H3K27me3-associated genes/pathways are affected in ethanol-exposed MSCs. • Expression of lineage-specific genes is dysregulated in ethanol-exposed MSCs. - Abstract: Fetal alcohol syndrome (FAS) is a birth defect due to maternal alcohol consumption during pregnancy. Because mesenchymal stem cells (MSCs) are the main somatic stem cells in adults and may contribute to tissue homeostasis and repair in adulthood, we investigated whether early life ethanol exposure affects MSCs and contributes to the propensity for disease onset in later life. Using a rodent model of FAS, we found that ethanol exposure (5.25 g/kg/day) from postnatal days 4 to 9 in rat pups (mimic of human third trimester) caused long-term anomalies in bone marrow-derived MSCs. MSCs isolated from ethanol-exposed animals were prone to neural induction but resistant to osteogenic and adipogenic inductions compared to their age-matched controls. The altered differentiation may contribute to the severe trabecular bone loss seen in ethanol-exposed animals at 3 months of age as well as overt growth retardation. Expression of alkaline phosphatase, osteocalcin, aP2, and PPARγ were substantially inhibited, but BDNF was up-regulated in MSCs isolated from ethanol-exposed 3 month-old animals. Several signaling pathways were distorted in ethanol-exposed MSCs via altered trimethylation at histone 3 lysine 27. These results demonstrate that early life ethanol exposure can have long-term impacts in rat MSCs by both genetic and epigenetic mechanisms.

  15. JV Task - 116 Selenium's Role in the Seafood Safety Issue

    SciTech Connect (OSTI)

    Nicholas Ralston; Laura Raymond


    Continuing studies under these three funded projects - (JV Task 77 The Health Implications of the Mercury-Selenium Interaction, JV Task 96 Investigating the Importance of the Mercury-Selenium Interaction, and JV Task 116 Selenium's Role in the Seafood Safety Issue) - were performed to determine the effects of different levels of dietary mercury and selenium on the growth and development of test animals, and related tissue analyses, to understand the protective benefits of dietary selenium in reference to low-level exposure to mercury. Maternal exposure to methylmercury from seafood has been found to cause neurodevelopmental harm in children. However, significant nutritional benefits will be lost if fish consumption is needlessly avoided. The results of these studies support the hypothesis that intracellular Se itself is the physiologically important biomolecule and that the harm of mercury toxicity arises when Hg abundance becomes great enough to bind a significant portion of intracellular Se in vulnerable tissues such as the brain. Formation of HgSe limits bioavailability of Se for synthesis of Se-dependent enzymes, particularly in brain tissues. When production of these enzymes is impaired, the loss of their numerous essential functions results in the signs and symptoms of Hg toxicity. The finding that one mole of Se protects against many moles of Hg indicates that its beneficial effect is not due to sequestration of mercury as HgSe but rather due to the biological activity of the Se. Therefore, the selenium content of seafoods must be considered along with their methylmercury contents in evaluating the effect of dietary exposure to mercury.

  16. Fetal weight at term influenced by H-2-associated loci

    SciTech Connect (OSTI)

    Tyan, M.L.


    Pregnant mice which in theory differ only in the region of the major histocompatibility complex (MHC) on chromosome 17 (C57BL/10, the inbred partner [host strain], and B10.D2, B10.BR, BI0.A, B10.A[2R], Bl0.A[5R], B10.A[15R] and B10.A[18R]) were sacrificed on the 11th and 18th days of gestation, and the fetuses were sexed and weighed. Fetuses from reciprocal crosses between B10.A and B10BR, B10.D2 and C57BL/10 were weighed and sexed on the 18th day of gestation. It was found that (i) fetal weights were not significantly different among the strains examined on day 11 (Bl0.BR, B10.A[15R] and B10.A[18R]), (ii) B10.BR fetuses of both sexes weighed significantly less than fetuses from the other strains on day 18, (iii) B10.D2 18-day-old male but not female fetuses were heavier than the males from the other strains (this difference was not present when corrections for litter size were made), (iv) the fetuses from the B10.A x B10.BR cross were the smallest, those from the B10.D2 x 810.A cross the largest, and those from the B10.A x C57BL/10 crosses intermediate, and (v) maternal effects were noted In the B10.A x B10.BR and B10.A x B10.D2 but not the B10.A x C57BL/10 crosses. The results suggest that there are two or more MHC associated loci that influence growth rate in late gestation. Among the candidate genes are Ped and Igfr II. 17 refs., 4 tabs.

  17. The effect of copper deficiency on fetal growth and liver anti-oxidant capacity in the Cohen diabetic rat model

    SciTech Connect (OSTI)

    Ergaz, Zivanit; Shoshani-Dror, Dana; Guillemin, Claire; Neeman-azulay, Meytal; Fudim, Liza; Weksler-Zangen, Sarah; Stodgell, Christopher J.; Miller, Richard K.; Ornoy, Asher


    High sucrose low copper diet induces fetal growth restriction in the three strains of the Cohen diabetic rats: an inbred copper deficient resistant (CDr), an inbred copper deficient sensitive (CDs that become diabetic on high sucrose low copper diet -HSD) and an outbred Wistar derived Sabra rats. Although those growth restricted fetuses also exhibit increased oxidative stress, antioxidants do not restore normal growth. In the present study, we evaluated the role of copper deficiency in the HSD induced fetal growth restriction by adding to the drinking water of the rats 1 ppm or 2 ppm of copper throughout their pregnancy. Fetal and placental growth in correlation with fetal liver copper content and anti-oxidant capacity was evaluated on day 21 of pregnancy. HSD compared to regular chow induced fetal growth restriction, which was most significant in the Cohen diabetic sensitive animals. The addition of 1 ppm and 2 ppm copper to the drinking water normalized fetal growth in a dose dependent manner and reduced the degree of hyperglycemia in the diabetes sensitive rats. The CDs fetuses responded to the HSD with lower catalase like activity, and less reduced superoxide dismutase levels compared to the Sabra strain, and had high malondialdehyde levels even when fed regular chow. Immunostaining was higher for nitrotyrosine among the CDr and higher for hypoxia factor 1 α among the CDs. We conclude that in our model of dietary-induced fetal growth restriction, copper deficiency plays a major etiologic role in the decrease of fetal growth and anti-oxidant capacity. -- Highlights: ► High sucrose low copper diet restricted fetal growth in the Cohen diabetic rat model ► Maternal copper blood levels directly correlated with fetal liver copper content ► Copper supplementation decreased embryonic resorption in the inbred strains ► Copper supplementation reduced hyperglycemia in the sucrose sensitive inbred strain ► Copper supplementation alleviated growth restriction and

  18. Effect of paternal age in achondroplasia, thanatophoric dysplasia, and osteogenesis imperfecta

    SciTech Connect (OSTI)

    Orioli, I.M.; Castilla, E.E.; Scarano, G.; Mastroiacovo, P.


    The paternal ages of nonfamilial cases of achondroplasia (AC) (n = 78), thanatophoric dysplasia (TD) (n = 64), and osteogenesis imperfecta (OI) (n = 106), were compared with those of matched controls, from an Italian Indagine Policentrica Italiana sulle Malformazioni Congenite (IPIMC) and a South American Estudio Colaborativo Latinoamericano de Malformaciones Congenitas (ECLAMC) series. The degree of paternal age effect on the origin of these dominant mutations differed among the three conditions. Mean paternal age was highly elevated in AC, 36.30 {plus_minus} 6.74 years in the IPIMC, and 37.19 {plus_minus} 10.53 years in the ECLAMC; less consistently elevated in TD, 33.60 {plus_minus} 7.08 years in the IPIMC, and 36.41 {plus_minus} 9.38 years in the ECLAMC; and only slightly elevated in OI in the ECLAMC, 31.15 {plus_minus} 9.25 years, but not in the IPIMC, 32.26 {plus_minus} 6.07 years. Increased maternal age or birth order in these conditions disappeared when corrected for paternal age. Approximately 50% of AC and TD cases, and only 30% of OI cases, were born to fathers above age 35 years. For AC and TD, the increase in relative incidence with paternal age fitted an exponential curve. The variability of paternal age effect in these new mutations could be due, among other reasons, to the high proportion of germ-line mosaicism in OI parents, or to the localization of the AC gene, mapped to the 4p16.3 region, in the neighborhood of an unstable DNA area. 28 refs., 1 fig., 6 tabs.

  19. Modeling mixtures of thyroid gland function disruptors in a vertebrate alternative model, the zebrafish eleutheroembryo

    SciTech Connect (OSTI)

    Thienpont, Benedicte; Barata, Carlos; Ralda, Demetrio


    Maternal thyroxine (T4) plays an essential role in fetal brain development, and even mild and transitory deficits in free-T4 in pregnant women can produce irreversible neurological effects in their offspring. Women of childbearing age are daily exposed to mixtures of chemicals disrupting the thyroid gland function (TGFDs) through the diet, drinking water, air and pharmaceuticals, which has raised the highest concern for the potential additive or synergic effects on the development of mild hypothyroxinemia during early pregnancy. Recently we demonstrated that zebrafish eleutheroembryos provide a suitable alternative model for screening chemicals impairing the thyroid hormone synthesis. The present study used the intrafollicular T4-content (IT4C) of zebrafish eleutheroembryos as integrative endpoint for testing the hypotheses that the effect of mixtures of TGFDs with a similar mode of action [inhibition of thyroid peroxidase (TPO)] was well predicted by a concentration addition concept (CA) model, whereas the response addition concept (RA) model predicted better the effect of dissimilarly acting binary mixtures of TGFDs [TPO-inhibitors and sodium-iodide symporter (NIS)-inhibitors]. However, CA model provided better prediction of joint effects than RA in five out of the six tested mixtures. The exception being the mixture MMI (TPO-inhibitor)-KClO{sub 4} (NIS-inhibitor) dosed at a fixed ratio of EC{sub 10} that provided similar CA and RA predictions and hence it was difficult to get any conclusive result. There results support the phenomenological similarity criterion stating that the concept of concentration addition could be extended to mixture constituents having common apical endpoints or common adverse outcomes. - Highlights: Potential synergic or additive effect of mixtures of chemicals on thyroid function. Zebrafish as alternative model for testing the effect of mixtures of goitrogens. Concentration addition seems to predict better the effect of mixtures

  20. Mono-2-ethylhexyl phthalate induces oxidative stress responses in human placental cells in vitro

    SciTech Connect (OSTI)

    Tetz, Lauren M.; Cheng, Adrienne A.; Korte, Cassandra S.; Giese, Roger W.; Wang, Poguang; Harris, Craig; Meeker, John D.; Loch-Caruso, Rita


    Di-2-ethylhexyl phthalate (DEHP) is an environmental contaminant commonly used as a plasticizer in polyvinyl chloride products. Exposure to DEHP has been linked to adverse pregnancy outcomes in humans including preterm birth, low birth-weight, and pregnancy loss. Although oxidative stress is linked to the pathology of adverse pregnancy outcomes, effects of DEHP metabolites, including the active metabolite, mono-2-ethylhexyl phthalate (MEHP), on oxidative stress responses in placental cells have not been previously evaluated. The objective of the current study is to identify MEHP-stimulated oxidative stress responses in human placental cells. We treated a human placental cell line, HTR-8/SVneo, with MEHP and then measured reactive oxygen species (ROS) generation using the dichlorofluorescein assay, oxidized thymine with mass-spectrometry, redox-sensitive gene expression with qRT-PCR, and apoptosis using a luminescence assay for caspase 3/7 activity. Treatment of HTR-8 cells with 180 ?M MEHP increased ROS generation, oxidative DNA damage, and caspase 3/7 activity, and resulted in differential expression of redox-sensitive genes. Notably, 90 and 180 ?M MEHP significantly induced mRNA expression of prostaglandin-endoperoxide synthase 2 (PTGS2), an enzyme important for synthesis of prostaglandins implicated in initiation of labor. The results from the present study are the first to demonstrate that MEHP stimulates oxidative stress responses in placental cells. Furthermore, the MEHP concentrations used were within an order of magnitude of the highest concentrations measured previously in human umbilical cord or maternal serum. The findings from the current study warrant future mechanistic studies of oxidative stress, apoptosis, and prostaglandins as molecular mediators of DEHP/MEHP-associated adverse pregnancy outcomes. - Highlights: ? MEHP increased reactive oxygen species, oxidative DNA damage, and caspase activity. ? MEHP induced expression of PTGS2, a gene important

  1. Fetal and neonatal exposure to nicotine leads to augmented hepatic and circulating triglycerides in adult male offspring due to increased expression of fatty acid synthase

    SciTech Connect (OSTI)

    Ma, Noelle; Nicholson, Catherine J.; Wong, Michael; Holloway, Alison C.; Hardy, Daniel B.


    While nicotine replacement therapy is assumed to be a safer alternative to smoking during pregnancy, the long-term consequences for the offspring remain elusive. Animal studies now suggest that maternal nicotine exposure during perinatal life leads to a wide range of adverse outcomes for the offspring including increased adiposity. The focus of this study was to investigate if nicotine exposure during pregnancy and lactation leads to alterations in hepatic triglyceride synthesis. Female Wistar rats were randomly assigned to receive daily subcutaneous injections of saline (vehicle) or nicotine bitartrate (1 mg/kg/day) for two weeks prior to mating until weaning. At postnatal day 180 (PND 180), nicotine exposed offspring exhibited significantly elevated levels of circulating and hepatic triglycerides in the male offspring. This was concomitant with increased expression of fatty acid synthase (FAS), the critical hepatic enzyme in de novo triglyceride synthesis. Given that FAS is regulated by the nuclear receptor Liver X receptor (LXR?), we measured LXR? expression in both control and nicotine-exposed offspring. Nicotine exposure during pregnancy and lactation led to an increase in hepatic LXR? protein expression and enriched binding to the putative LXRE element on the FAS promoter in PND 180 male offspring. This was also associated with significantly enhanced acetylation of histone H3 [K9,14] surrounding the FAS promoter, a hallmark of chromatin activation. Collectively, these findings suggest that nicotine exposure during pregnancy and lactation leads to an increase in circulating and hepatic triglycerides long-term via changes in the transcriptional and epigenetic regulation of the hepatic lipogenic pathway. - Highlights: Our data reveals the links nicotine exposure in utero and long-term hypertriglyceridemia. It is due to nicotine-induced augmented expression of hepatic FAS and LXR? activity. Moreover, this involves nicotine-induced enhanced acetylation of

  2. X-linked inheritance in neuronal migration disorders (NMD)

    SciTech Connect (OSTI)

    Andermann, E.; Dubeau, F.; Tampieri, D.


    With the advent of MRI imaging, an increasing number of NMD have been identified in patients with epilepsy. Although most cases have been sporadic, families with these disorders have now been reported in several types of NMD. Furthermore, subcortical bank heterotopia (SBH) or {open_quotes}double cortex syndrome{close_quotes} and periventricular nodular heterotopia (PNH) have a marked female predominance. Two females with SBH, mild mental retardation and seizures had sons with lissencephaly, severe retardation and seizures, and daughters with SBH. X-linked lissencephaly has been observed in several other families, and one girl with lissencephaly was found to have a de novo X-autosomal translocation with a breakpoint in chromosome Xq22. We have studied three families with two or more generations affected by PNH in females, a high frequency of spontaneous abortions and abnormal sex ratios in sibships. The clinical manifestations include seizures and normal intelligence. Three other families with PNH in females have been reported in the literature. Bilateral perisylvian polymicrogyria has been reported in monozygotic twins and in siblings, and we have studied a brother and sister with an affected maternal uncle. These findings suggest sex-linked dominant inheritance with male lethality or severe expression in males. The three disorders described above may represent different mutations of a single gene or mutations in two or more genes on the X-chromosome. At least one gene is probably located in chromosome band Xq22. Genetic linkage studies in families with NMD as well as a search for candidate genes such as adhesion molecules known to map on the X-chromosome should lead to the identification of the gene(s) responsible for these disorders.

  3. A human factors approach towards the design of a new glovebox glove for Los Alamos National Laboratory

    SciTech Connect (OSTI)

    Oka, Jude M.


    thumb for better positioning, 45 degrees extension angle for the thumb for better positioning, and various angles for the other fingers to allow a more relaxed and natural fit. 3D modeling was used to implement the anthropometric data listed above onto an existing scanned solid model of a human hand. SolidWorks 2010 3-D modeling package was utilized to manipulate the hand model to represent the anthropometric data researched. The anthropometrics and modifications were reviewed by the University of New Mexico Department of Orthopedics hand surgeons. After all modifications and reviews were completed the model was printed out using stereolithography. The printed out model of the hand was used as a mold to create a prototype glovebox glove. The new mold was taken to Piercan USA to produce a 20mil Polyurethane/Hypalon glovebox glove. The Minnesota Dexterity test and Purdue Pegboard test were used to measure the dexterity of the prototype glovebox glove against a current 15 mil Hypalon LANL glovebox glove. Using the data from the tests a student t test was used to determine if there was a significant difference between the current hypalon glove results and the new prototype glove results. With a 95% confidence level the prototype showed to have a significantly lower mean difference from the current hypalon glovebox glove with the Minnesota Dexterity test. With a 95% confidence level the prototype showed to have a significantly higher mean difference from the current hypalon glovebox glove with the Purdue Pegboard test. A p value method was also performed to confirm the results of the student t test. A survey was also given to glovebox workers to determine if they preferred the new design. The best reaction from glovebox workers was the new thumb position, 73.2% of the sample population agreed with the new thumb position. Developing a new glovebox glove will improve the ergonomics of the hand for work performed, decrease exposure time, decreasing risk of breaching, increasing

  4. DEFORMATION AND FRACTURE OF POORLY CONSOLIDATED MEDIA - Borehole Failure Mechanisms in High-Porosity Sandstone

    SciTech Connect (OSTI)

    Bezalel c. Haimson


    We investigated failure mechanisms around boreholes and the formation of borehole breakouts in high-porosity sandstone, with particular interest to grain-scale micromechanics of failure leading to the hitherto unrecognized fracture-like borehole breakouts and apparent compaction band formation in poorly consolidated granular materials. We also looked at a variety of drilling-related factors that contribute to the type, size and shape of borehole breakouts. The objective was to assess their effect on the ability to establish correlations between breakout geometry and in situ stress magnitudes, as well as on borehole stability prediction, and hydrocarbon/water extraction in general. We identified two classes of medium to high porosity (12-30%) sandstones, arkosic, consisting of 50-70% quartz and 15 to 50% feldspar, and quartz-rich sandstones, in which quartz grain contents varied from 90 to 100%. In arkose sandstones critical far-field stress magnitudes induced compressive failure around boreholes in the form of V-shaped (dog-eared) breakouts, the result of dilatant intra-and trans-granular microcracking subparallel to both the maximum horizontal far-field stress and to the borehole wall. On the other hand, boreholes in quartz-rich sandstones failed by developing fracture-like breakouts. These are long and very narrow (several grain diameters) tabular failure zones perpendicular to the maximum stress. Evidence provided mainly by SEM observations suggests a failure process initiated by localized grain-bond loosening along the least horizontal far-field stress springline, the packing of these grains into a lower porosity compaction band resembling those discovered in Navajo and Aztec sandstones, and the emptying of the loosened grains by the circulating drilling fluid starting from the borehole wall. Although the immediate several grain layers at the breakout tip often contain some cracked or even crushed grains, the failure mechanism enabled by the formation of the

  5. ISCR Annual Report: Fical Year 2004

    SciTech Connect (OSTI)

    McGraw, J R


    long-term visits with the aim of encouraging long-term academic research agendas that address LLNL's research priorities. Through such collaborations, ideas and software flow in both directions, and LLNL cultivates its future workforce. The Institute strives to be LLNL's ''eyes and ears'' in the computer and information sciences, keeping the Laboratory aware of and connected to important external advances. It also attempts to be the ''feet and hands'' that carry those advances into the Laboratory and incorporates them into practice. ISCR research participants are integrated into LLNL's Computing and Applied Research (CAR) Department, especially into its Center for Applied Scientific Computing (CASC). In turn, these organizations address computational challenges arising throughout the rest of the Laboratory. Administratively, the ISCR flourishes under LLNL's University Relations Program (URP). Together with the other five institutes of the URP, it navigates a course that allows LLNL to benefit from academic exchanges while preserving national security. While it is difficult to operate an academic-like research enterprise within the context of a national security laboratory, the results declare the challenges well met and worth the continued effort.

  6. Exposure to mercury among Spanish preschool children: Trend from birth to age four

    SciTech Connect (OSTI)

    Llop, Sabrina; Murcia, Mario; Aguinagalde, Xabier; Vioque, Jesus; Rebagliato, Marisa; Iñiguez, Carmen; Lopez-Espinosa, Maria-Jose; Amurrio, Ascensión; María Navarrete-Muñoz, Eva; and others


    The purpose of this study is to describe the total hair mercury concentrations and their determinants in preschool Spanish children, as well as to explore the trend in mercury exposure from birth to the age four. This evolution has been scarcely studied in other birth cohort studies. The study population was 580 four year old children participating in the INMA (i.e. Childhood and Environment) birth cohort study in Valencia (2008–2009). Total mercury concentration at age four was measured in hair samples by atomic absorption spectrometry. Fish consumption and other covariates were obtained by questionnaire. Multivariate linear regression models were conducted in order to explore the association between mercury exposure and fish consumption, socio-demographic characteristics and prenatal exposure to mercury. The geometric mean was 1.10 µg/g (95%CI: 1.02, 1.19). Nineteen percent of children had mercury concentrations above the equivalent to the Provisional Tolerable Weekly Intake proposed by WHO. Mercury concentration was associated with increasing maternal age, fish consumption and cord blood mercury levels, as well as decreasing parity. Children whose mothers worked had higher mercury levels than those with non working mothers. Swordfish, lean fish and canned fish were the fish categories most associated with hair mercury concentrations. We observed a decreasing trend in mercury concentrations between birth and age four. In conclusion, the children participating in this study had high hair mercury concentrations compared to reported studies on children from other European countries and similar to other countries with high fish consumption. The INMA study design allows the evaluation of the exposure to mercury longitudinally and enables this information to be used for biomonitoring purposes and dietary recommendations. - Highlights: • The geometric mean of hair Hg concentrations was 1.10 µg/g. • 19% of children had Hg concentrations above the RfD proposed by

  7. Transcription Factors Bind Thousands of Active and InactiveRegions in the Drosophila Blastoderm

    SciTech Connect (OSTI)

    Li, Xiao-Yong; MacArthur, Stewart; Bourgon, Richard; Nix, David; Pollard, Daniel A.; Iyer, Venky N.; Hechmer, Aaron; Simirenko, Lisa; Stapleton, Mark; Luengo Hendriks, Cris L.; Chu, Hou Cheng; Ogawa, Nobuo; Inwood, William; Sementchenko, Victor; Beaton, Amy; Weiszmann, Richard; Celniker, Susan E.; Knowles, David W.; Gingeras, Tom; Speed, Terence P.; Eisen, Michael B.; Biggin, Mark D.


    Identifying the genomic regions bound by sequence-specific regulatory factors is central both to deciphering the complex DNA cis-regulatory code that controls transcription in metazoans and to determining the range of genes that shape animal morphogenesis. Here, we use whole-genome tiling arrays to map sequences bound in Drosophila melanogaster embryos by the six maternal and gap transcription factors that initiate anterior-posterior patterning. We find that these sequence-specific DNA binding proteins bind with quantitatively different specificities to highly overlapping sets of several thousand genomic regions in blastoderm embryos. Specific high- and moderate-affinity in vitro recognition sequences for each factor are enriched in bound regions. This enrichment, however, is not sufficient to explain the pattern of binding in vivo and varies in a context-dependent manner, demonstrating that higher-order rules must govern targeting of transcription factors. The more highly bound regions include all of the over forty well-characterized enhancers known to respond to these factors as well as several hundred putative new cis-regulatory modules clustered near developmental regulators and other genes with patterned expression at this stage of embryogenesis. The new targets include most of the microRNAs (miRNAs) transcribed in the blastoderm, as well as all major zygotically transcribed dorsal-ventral patterning genes, whose expression we show to be quantitatively modulated by anterior-posterior factors. In addition to these highly bound regions, there are several thousand regions that are reproducibly bound at lower levels. However, these poorly bound regions are, collectively, far more distant from genes transcribed in the blastoderm than highly bound regions; are preferentially found in protein-coding sequences; and are less conserved than highly bound regions. Together these observations suggest that many of these poorly-bound regions are not involved in early

  8. Imprinted genes and transpositions: epigenomic targets for low dose radiation effects. Final report

    SciTech Connect (OSTI)

    Jirtle, Randy L.


    The overall hypothesis of this grant application is that low dose ionizing radiation (LDIR) elicits adaptive responses in part by causing heritable DNA methylation changes in the epigenome. This novel postulate was tested by determining if the level of DNA methylation at the Agouti viable yellow (A{sup vy}) metastable locus is altered, in a dose-dependent manner, by low dose radiation exposure (<10 cGy) during early gestation. This information is particularly important to ascertain given the increased use of CT scans in disease diagnosis, increased number of people predicted to live and work in space, and the present concern about radiological terrorism. We showed for the first time that LDIR significantly increased DNA methylation at the A{sup vy} locus in a sex-specific manner (p=0.004). Average DNA methylation was significantly increased in male offspring exposed to doses between 0.7 cGy and 7.6 cGy with maximum effects at 1.4 cGy and 3.0 cGy (p<0.01). Offspring coat color was concomitantly shifted towards pseudoagouti (p<0.01). Maternal dietary antioxidant supplementation mitigated both the DNA methylation changes and coat color shift in the irradiated offspring (p<0.05). Thus, LDIR exposure during gestation elicits epigenetic alterations that lead to positive adaptive phenotypic changes that are negated with antioxidants, indicating they are mediated in part by oxidative stress. These findings provide evidence that in the isogenic Avy mouse model epigenetic alterations resulting from LDIR play a role in radiation hormesis, bringing into question the assumption that every dose of radiation is harmful. Our findings not only have significant implications concerning the mechanism of hormesis, but they also emphasize the potential importance of this phenomenon in determining human risk at low radiation doses. Since the epigenetic regulation of genes varies markedly between species, the effect of LDIR on other epigenetically labile genes (e.g. imprinted genes) in

  9. Body burdens of brominated flame retardants and other persistent organo-halogenated compounds and their descriptors in US girls

    SciTech Connect (OSTI)

    Windham, Gayle C.; Pinney, Susan M.; Sjodin, Andreas; Lum, Raymond; Jones, Richard S.; Needham, Larry L.; Biro, Frank M.; Hiatt, Robert A.; Kushi, Lawrence H.


    Background: Levels of brominated flame retardants are increasing in US populations, yet little data are available on body burdens of these and other persistent hormonally active agents (HAAs) in school-aged children. Exposures to such chemicals may affect a number of health outcomes related to development and reproductive function. Objective: Determine the distribution of biomarkers of polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs), and organo-chlorinated pesticides (OCPs), such as DDT/DDE, in children, and their variation by key descriptor variables. Methods: Ethnically diverse cohorts of girls 6-8 y old at baseline are being followed for growth and pubertal development in a multi-site, longitudinal study. Nearly 600 serum samples from the California and Ohio sites were analyzed for lipids, 35 PCB congeners, 11 PBDE congeners, and 9 OCPs. The biomarker distributions were examined and geometric means compared for selected analytes across categories of age, race, site, body mass index (BMI), parental education, maternal age at delivery, and breast feeding in adjusted models. Results: Six PBDE congeners were detected among greater than 70% of samples, with BDE-47 having the highest concentration (median 42.2, range 4.9-855 ng/g lipid). Girls in California had adjusted geometric mean (GM) PBDE levels significantly higher than girls in Ohio. Furthermore, Blacks had significantly higher adjusted GMs of all six PBDE congeners than Whites, and Hispanics had intermediate values. GMs tended to be lower among more obese girls, while other variables were not strongly associated. In contrast, GMs of the six PCB congeners most frequently detected were significantly lower among Blacks and Hispanics than Whites. PCBs and the three pesticides most frequently detected were also consistently lower among girls with high BMI, who were not breast-fed, whose mothers were younger, or whose care-givers (usually parents) were less educated. Girls in California had

  10. Pregnancy and Parenthood in Radiation Oncology, Views and Experiences Survey (PROVES): Results of a Blinded Prospective Trainee Parenting and Career Development Assessment

    SciTech Connect (OSTI)

    Holliday, Emma B.; Ahmed, Awad A.; Jagsi, Reshma; Stentz, Natalie Clark; Woodward, Wendy A.; Fuller, Clifton D.; Thomas, Charles R.


    Purpose: Medical training spans nearly a decade, during which many physicians traditionally begin families. Although childrearing responsibilities are shared by men and women in the modern era, differences in time allocated to child care by sex and its potential impact on residency experience merit discussion. Methods and Materials: An anonymous, voluntary, 102-item survey was distributed to 540 current radiation oncology residents and 2014 graduates that asked about marital and parental status, pregnancy during residency, publication productivity, career aspirations, and experiences working with pregnant co-residents. Respondents with children were asked about childcare arrangements, and women who were pregnant during residency were asked about radiation safety, maternity leave, and breastfeeding experiences. Results: A total of 190 respondents completed the survey, 107 men (56.3%) and 84 women (43.7%). Ninety-seven respondents (51.1%) were parents, and 84 (44.2%) reported a pregnancy during residency. Respondents with children more often were male (65% vs 47.3%; P=.014), in a higher level of training (79.3% vs 54.8% were PGY4 or higher; P=.001), were older (median age of 32, interquartile range [IQR]:31-35] vs age 30 [IQR: 29-33]; P<.001), had a PhD (33% vs 19.3%, respectively; P=.033), were married (99% vs 43%, respectively; P<.001), and had a partner who did not work (24.7% vs 1.9%, respectively; <.001). There were no differences in the number of manuscripts published or the number of residents who expressed likelihood of pursing an academic career by parental status. Among parents, men more frequently had partners who did not work (38.1% vs 0%, respectively; P<.001) and reported that their partner performed a greater percentage of childcare duties (70% [IQR: 60%-80%] vs 35% [IQR: 20%-50%], respectively; P<.001). Conclusions: Pregnancy and parenthood are common during residency. Female residents are frequently responsible for more childcare duties than males

  11. Transplacental arsenic carcinogenesis in mice

    SciTech Connect (OSTI)

    Waalkes, Michael P. Liu, Jie; Diwan, Bhalchandra A.


    Our work has focused on the carcinogenic effects of in utero arsenic exposure in mice. Our data show that a short period of maternal exposure to inorganic arsenic in the drinking water is an effective, multi-tissue carcinogen in the adult offspring. These studies have been reproduced in three temporally separate studies using two different mouse strains. In these studies pregnant mice were treated with drinking water containing sodium arsenite at up to 85 ppm arsenic from days 8 to 18 of gestation, and the offspring were observed for up to 2 years. The doses used in all these studies were well tolerated by both the dam and offspring. In C3H mice, two separate studies show male offspring exposed to arsenic in utero developed liver carcinoma and adrenal cortical adenoma in a dose-related fashion during adulthood. Prenatally exposed female C3H offspring show dose-related increases in ovarian tumors and lung carcinoma and in proliferative lesions (tumors plus preneoplastic hyperplasia) of the uterus and oviduct. In addition, prenatal arsenic plus postnatal exposure to the tumor promoter, 12-O-tetradecanoyl phorbol-13-acetate (TPA) in C3H mice produces excess lung tumors in both sexes and liver tumors in females. Male CD1 mice treated with arsenic in utero develop tumors of the liver and adrenal and renal hyperplasia while females develop tumors of urogenital system, ovary, uterus and adrenal and hyperplasia of the oviduct. Additional postnatal treatment with diethylstilbestrol or tamoxifen after prenatal arsenic in CD1 mice induces urinary bladder transitional cell proliferative lesions, including carcinoma and papilloma, and enhances the carcinogenic response in the liver of both sexes. Overall this model has provided convincing evidence that arsenic is a transplacental carcinogen in mice with the ability to target tissues of potential human relevance, such as the urinary bladder, lung and liver. Transplacental carcinogenesis clearly occurs with other agents in humans

  12. Enhanced vector borne disease surveillance of California Culex mosquito populations reveals spatial and species-specific barriers of infection.

    SciTech Connect (OSTI)

    VanderNoot, Victoria A.; Curtis, Deanna Joy; Koh, Chung-Yan; Brodsky, Benjamin H; Lane, Todd


    Monitor i ng in f ectio n s in v ect o rs su c h as m osquit o es, s a nd fl i es, tsetse fl i es, a nd ticks to i denti f y hu m a n path o gens m a y s e r v e as a n ear l y w arn i ng det e ction system t o dir e ct loc a l g o v er n ment dise a se pr e v en t i v e m easu r e s . One major hurdle i n de t ection is the abi l i t y to scre e n l arge n u mbers of v e c t ors for h uman patho g ens w i thout t h e u s e of ge n o t y pe - s p ecific m o lecu l ar tec h nique s . N e x t genera t ion s equ e nc i ng (NG S ) pr o v i des a n unbi a sed p latfo r m capab l e of identi f y i ng k n o w n a n d unk n o w n p ath o ge n s circula t ing w i thin a v e ctor p opul a tion, but utili z ing t h is te c h nolo g y i s tim e - con s u ming a n d cos t l y for v ecto r -b o rne disease su r v e illan c e pr o gra m s. T o addr e s s this w e d e v e lop e d cos t -eff e ct i v e Ilumina(r) R NA- S eq l i bra r y p r epara t ion m e thodol o gies i n con j u n ction w i t h an automa t ed c ompu t at i onal a n a l y sis pipel i n e to ch a racter i ze t h e microbial popula t ions c ircula t i n g in Cu l e x m o squit o e s (Cul e x qui n quef a s c iatu s , C ul e x quinq u efasc i atus / pip i ens co m pl e x h y bri d s, and C u l e x ta r salis ) t hroug h out Californ i a. W e assembled 2 0 n o vel a n d w e l l -do c ume n ted a r b o v i ruses repres e nting mem b e rs of B u n y a v ir i da e , F l a v i virid a e, If a virida e , Meson i v i rida e , Nid o v iri d ae, O rtho m y x o virid a e, Pa r v o v iri d ae, Re o virid a e, R h a b d o v i rid a e, T y m o v iri d ae, a s w ell as s e v e r al u n assi g n e d v irus e s . In addit i o n, w e m app e d mRNA s pecies to d i vergent s peci e s of t r y panos o ma a nd pl a s modium eu k a r yotic parasit e s and cha r a c terized t he p r oka r yot i c microb i al c o mposit i on to i d enti f y bacteri a l tran s c r ipts der i v ed from wolba c hia, clo s tridi u m, m y c oplas m a, fusoba c


    SciTech Connect (OSTI)

    Greeley Jr, Mark Stephen; Elmore, Logan R; McCracken, Kitty


    On December 22, 2008, a dike containing fly ash and bottom ash in an 84-acre complex of the Tennessee Valley Authority's (TVA) Kingston Steam Plant in East Tennessee failed and released a large quantity of ash into the adjacent Emory River. Ash deposits extended as far as 4 miles upstream (Emory River mile 6) of the Plant, and some ash was carried as far downstream as Tennessee River mile 564 ({approx}4 miles downstream of the Tennessee River confluence with the Clinch River). A byproduct of coal burning power plants, fly ash contains a variety of metals and other elements which, at sufficient concentrations and in specific forms, can be toxic to biological systems. The effects of fly ash contamination on exposed fish populations depend on the magnitude and duration of exposure, with the most significant risk considered to be the effects of specific ash constituents, especially selenium, on fish early life stages. Uptake by adult female fish of fly ash constituents through the food chain and subsequent maternal transfer of contaminants to the developing eggs is thought to be the primary route of selenium exposure to larval fish (Woock and others 1987, Coyle and others 1993, Lemly 1999, Moscatello and others 2006), but direct contact of the fertilized eggs and developing embryos to ash constituents in river water and sediments is also a potential risk factor (Woock and others 1987, Coyle and others 1993, Jezierska and others 2009). To address the risk of fly ash from the Kingston spill to the reproductive health of downstream fish populations, ORNL has undertaken a series of studies in collaboration with TVA including: (1) a field study of the bioaccumulation of fly ash constituents in fish ovaries and the reproductive condition of sentinel fish species in reaches of the Emory and Clinch Rivers affected by the fly ash spill; (2) laboratory tests of the potential toxicity of fly ash from the spill area on fish embryonic and larval development (reported in the current

  14. Energy Systems and Population Health

    SciTech Connect (OSTI)

    Ezzati, Majid; Bailis, Rob; Kammen, Daniel M.; Holloway, Tracey; Price, Lynn; Cifuentes, Luis A.; Barnes, Brendon; Chaurey, Akanksha; Dhanapala, Kiran N.


    to rural and urban health facilities allows increased delivery and coverage of 3 various health services and interventions such as tests and treatments, better storage of medicine and vaccines, disinfection of medical equipment by boiling or radiation, and more frequent and efficient health system encounters through mobile clinics or longer working hours; and so on. In fact, while the dominant view of development-energy-health linkages has been that improvements in energy and health are outcomes of the socioeconomic development process (e.g., the ''energy ladder'' framework discussed below), it has even been argued that access to higher quality energy sources and technologies can initiate a chain of demographic, health, and development outcomes by changing the household structure and socioeconomic relationships. For example, in addition to increased opportunities for food and income production, reduced infant mortality as a result of transition to cleaner fuels or increased coverage of vaccination with availability of refrigerators in rural clinics may initiate a process of ''demographic transition'' to low-mortality and low-fertility populations (14). Such a transition has historically been followed with further improvements in maternal and child health and increased female participation in the labor markets and other economic activities.

  15. Evaluating the Effects of the Kingston Fly Ash Release on Fish Reproduction: Spring 2009 - 2010 Studies

    SciTech Connect (OSTI)

    Greeley Jr, Mark Stephen; Adams, Marshall; McCracken, Kitty


    On December 22, 2008, a dike containing fly ash and bottom ash at the Tennessee Valley Authority's (TVA) Kingston Fossil Plant in East Tennessee failed and released a large quantity of ash into the adjacent Emory River. Ash deposits from the spill extended 4 miles upstream of the facility to Emory River mile 6 and downstream to Tennessee River mile 564 ({approx}8.5 miles downstream of the confluence of the Emory River with the Clinch River, and {approx}4 miles downstream of the confluence of the Clinch River with the Tennessee River). A byproduct of coal combustion, fly ash contains a variety of metals and other elements which, at sufficient concentrations and in specific forms, can be harmful to biological systems. The ecological effects of fly ash contamination on exposed fish populations depend on the magnitude and duration of exposure, with the most significant risk considered to come from elevated levels of certain metals in the ash, particularly selenium, on fish reproduction and fish early life stages (Lemly 1993; Besser and others 1996). The ovaries of adult female fish in a lake contaminated by coal ash were reported to have an increased frequency of atretic oocytes (dead or damaged immature eggs) and reductions in the overall numbers of developing oocytes (Sorensen 1988) associated with elevated body burdens of selenium. Larval fish exposed to selenium through maternal transfer of contaminants to developing eggs in either contaminated bodies of water (Lemly 1999) or in experimental laboratory exposures (Woock and others 1987, Jezierska and others 2009) have significantly increased incidences of developmental abnormalities. Contact of fertilized eggs and developing embryos to ash in water and sediments may also pose an additional risk to the early life stages of exposed fish populations through direct uptake of metals and other ash constituents (Jezierska and others 2009). The establishment and maintenance of fish populations is intimately associated with

  16. Worksheet

    U.S. Energy Information Administration (EIA) (indexed site)

    ;Western Area Power Admin",3,"Structure 96/4","O/Banion 1",100,38,"OH","AC",230,230,,"ASCR",,2,2,"Steel Lattice",,0,0,0,0,0 2003,27000,"Western Area Power Admin",4,"Mead","Market Place",100,12.9,"OH","AC",525,525,,"ASCR",,1,1,"Steel Lattice",,0,0,0,0,0 2003,27000,"Western Area Power Admin",5,"Bears Ears","Craig",100,1,"OH","AC",345,345,,"ASCR",,1,1,"Steel Lattice",,0,0,0,0,0 2003,27000,"Western Area Power Admin",6,"Glen Canyon Pumping Plant","Glen Canyon SW Yard",100,1,"OH","AC",345,345,,"ASCR",,1,1,"Steel Lattice",,0,0,0,0,0 2003,27000,"Western Area Power Admin",7,"Baker","Bowman",22.96,53.96,"OH","AC",230,230,954,"ASCR",,1,1,"Wood H",,0,0,0,0,0 2003,27000,"Western Area Power Admin",8,"Basin Tap #2","Washburn",100,2.23,"OH","AC",230,230,795,"ASCR",,1,1,"Steel Lattice",,0,0,0,0,0 2003,27000,"Western Area Power Admin",9,"Craig","Rifle",100,96,"OH","AC",230,230,1272,"ASCR",,1,1,"Steel Lattice",,0,0,0,0,0 2003,27000,"Western Area Power Admin",10,"Garrison","Basin Tap #1",100,20.97,"OH","AC",230,230,795,"ASCR",,1,1,"Steel Lattice",,0,0,0,0,0 2003,27000,"Western Area Power Admin",11,"Everta","Roseville",100,13.3,"OH","AC",230,230,,"ASCR",,1,1,"Steel Lattice",,0,0,0,0,0 2003,27000,"Western Area Power Admin",12,"Griffith","McConnico",100,8,"OH","AC",230,230,1272,"ASCR",,1,1,"Steel Lattice",,0