National Library of Energy BETA

Sample records for mi investor owned

  1. Financial statistics of selected investor-owned electric utilities, 1989

    SciTech Connect (OSTI)

    Not Available

    1991-01-01

    The Financial Statistics of Selected Investor-Owned Electric Utilities publication presents summary and detailed financial accounting data on the investor-owned electric utilities. The objective of the publication is to provide the Federal and State governments, industry, and the general public with current and historical data that can be used for policymaking and decisionmaking purposes related to investor-owned electric utility issues.

  2. Financial Statistics of Major U.S. Investor-Owned Electric Utilities

    Reports and Publications

    1997-01-01

    1996 - Final issue. Presents summary and detailed financial accounting data on the investor-owned electric utilities.

  3. Financial statistics of major U.S. investor-owned electric utilities 1993

    SciTech Connect (OSTI)

    Not Available

    1995-01-01

    The Financial Statistics of Major US Investor-Owned Electric Utilities publication presents summary and detailed financial accounting data on the investor-owned electric utilities. The objective of the publication is to provide Federal and State governments, industry, and the general public with current and historical data that can be used for policymaking and decisionmaking purposes related to investor-owned electric utility issues.

  4. Financial statistics of major US investor-owned electric utilities 1994

    SciTech Connect (OSTI)

    1995-12-01

    The Financial Statistics of Major U.S. Investor-Owned Electric Utilities publication presents summary and detailed financial accounting data on the investor-owned electric utilities. The objective of the publication is to provide Federal and State Governments, industry, and the general public with current and historical data that can be used for making policy and decisions relating to investor-owned electric utility issues.

  5. Financial statistics of major US investor-owned electric utilities 1992

    SciTech Connect (OSTI)

    Not Available

    1993-12-28

    The Financial Statistics of Major US Investor-Owned Electric Utilities publication presents summary and detailed financial accounting data on the investor-owned electric utilities. The objective of the publication is to provide Federal and State governments, industry, and the general public with current and historical data that can be used for policymaking and decisionmaking purposes related to investor-owned electric utility issues. The Financial Statistics of Major US Investor-Owned Electric Utilities publication provides information about the financial results of operations of investor-owned electric utilities for use by government, industry, electric utilities, financial organizations and educational institutions in energy planning. In the private sector, the readers of this publication are researchers and analysts associated with the financial markets, the policymaking and decisionmaking members of electric utility companies, and economic development organizations. Other organizations that may be interested in the data presented in this publication include manufacturers of electric power equipment and marketing organizations. In the public sector, the readers of this publication include analysts, researchers, statisticians, and other professionals engaged in regulatory, policy, and program areas. These individuals are generally associated with the Congress, other legislative bodies, State public utility commissions, universities, and national strategic planning organizations.

  6. Financial impacts of nonutility power purchases on investor-owned electric utilities

    SciTech Connect (OSTI)

    Not Available

    1994-06-01

    To assist in its these responsibilities in the area of electric power, EIA has prepared this report, Financial Impacts of Nonutility Power Purchases on Investor-Owned Electric Utilities. The primary purpose of this report is to provide an overview of the issues surrounding the financial impacts of nonutility generation contracts (since the passage of the Public Utility Regulatory Policies Act of 1978) on investor-owned utilities. The existing concern in this area is manifest in the provisions of Section 712 of the Energy Policy Act of 1992, which required State regulatory commissions to evaluate various aspects of long-term power purchase contracts, including their impact on investor-owned utilities` cost of capital and rates charged to customers. The EIA does not take positions on policy questions. The EIA`s responsibility is to provide timely, high quality information and to perform objective, credible analyses in support of the deliberations by both public and private decision-makers. Accordingly, this report does not purport to represent the policy positions of the US Department of Energy or the Administration.

  7. Revenue and earnings performance masked continuing investor-owned utility problems

    SciTech Connect (OSTI)

    Lincicome, R.A.

    1983-06-01

    The 1982 increase in revenues and net income for the top 100 electric utilities is misleading because the figure is distorted by the allowance for funds used during construction (AFUDC), which overstates the real dollar strength of most investor-owned utilities. A random sampling of profit and loss statements shows that companies heavily involved in plant construction can have AFUDC over 100% of net income. The average is 50% of utility earnings, while cash dividends run 75% of earnings. The problem is short-term, however, and will diminish as construction is completed. A summary of utility performance presents earnings growth statistics, sales data and comparisons, financial statistics, and income statistics and comparisons. A summary financial table lists the 100 utilities in alphabetical order. 7 tables. (DCK)

  8. Incentive regulation of investor-owned nuclear power plants by public utility regulators. Revision 1

    SciTech Connect (OSTI)

    McKinney, M.D.; Seely, H.E.; Merritt, C.R.; Baker, D.C.

    1995-04-01

    The US Nuclear Regulatory Commission (NRC) periodically surveys the Federal Energy Regulatory Commission (FERC) and state regulatory commissions that regulate utility owners of nuclear power plants. The NRC is interested in identifying states that have established economic or performance incentive programs applicable to nuclear power plants, how the programs are being implemented, and in determining the financial impact of the programs on the utilities. The NRC interest stems from the fact that such programs have the potential to adversely affect the safety of nuclear power plants. The current report is an update of NUREG/CR-5975, Incentive Regulation of Investor-Owned Nuclear Power Plants by Public Utility Regulators, published in January 1993. The information in this report was obtained from interviews conducted with each state regulatory agency that administers an incentive program and each utility that owns at least 10% of an affected nuclear power plant. The agreements, orders, and settlements that form the basis for each incentive program were reviewed as required. The interviews and supporting documentation form the basis for the individual state reports describing the structure and financial impact of each incentive program.

  9. Investor Relations

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    and related services at cost. BPA Overview for Investors - as of September 24, 2015 Credit Ratings Latest Rating Agency Reports Full Reports: Fitch Full Report, March 2014...

  10. Owned","Public","Federal","Cooperative","Non-utility","Energy...

    U.S. Energy Information Administration (EIA) (indexed site)

    Florida" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of ...

  11. Owned","Public","Federal","Cooperative","Non-utility","Energy...

    U.S. Energy Information Administration (EIA) (indexed site)

    Nevada" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of ...

  12. Owned","Public","Federal","Cooperative","Non-utility","Energy...

    U.S. Energy Information Administration (EIA) (indexed site)

    Mexico" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of ...

  13. Owned","Public","Federal","Cooperative","Non-utility","Energy...

    U.S. Energy Information Administration (EIA) (indexed site)

    Minnesota" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of ...

  14. Low Carbon Investors Ltd | Open Energy Information

    Open Energy Information (Open El) [EERE & EIA]

    Investors Ltd Jump to: navigation, search Name: Low Carbon Investors Ltd Place: London, England, United Kingdom Zip: W1W 7TH Sector: Carbon Product: London-based manager of the Low...

  15. Khosla Vinod Private investor | Open Energy Information

    Open Energy Information (Open El) [EERE & EIA]

    Place: Menlo Park, California Zip: 94025 Product: Individual investor providing strategic advice and venture capital to start-ups. References: Khosla, Vinod (Private...

  16. West Central Biodiesel Investors LLC | Open Energy Information

    Open Energy Information (Open El) [EERE & EIA]

    Biodiesel Investors LLC Jump to: navigation, search Name: West Central Biodiesel Investors, LLC Place: Ralston, Iowa Zip: 51459 Product: Iowa-based body raising capital to support...

  17. Quercus Trust David Gelbaum Private investor | Open Energy Information

    Open Energy Information (Open El) [EERE & EIA]

    Quercus Trust David Gelbaum Private investor Jump to: navigation, search Name: Quercus Trust David Gelbaum (Private investor) Place: Newport Beach, California Zip: 92660 Product:...

  18. Solar Energy LLC Industrial Investors Group | Open Energy Information

    Open Energy Information (Open El) [EERE & EIA]

    LLC Industrial Investors Group Jump to: navigation, search Name: Solar Energy LLC - Industrial Investors Group Place: Moscow, Russian Federation Zip: 119017 Sector: Solar Product:...

  19. Institutional Investors Group on Climate Change IIGCC | Open...

    Open Energy Information (Open El) [EERE & EIA]

    Institutional Investors Group on Climate Change IIGCC Jump to: navigation, search Name: Institutional Investors Group on Climate Change (IIGCC) Place: London, United Kingdom Zip:...

  20. Global Energy Investors | Open Energy Information

    Open Energy Information (Open El) [EERE & EIA]

    Wind energy Product: Massachusetts-based Global Energy Investors was formed in 2009 to invest primarily in wind and solar power projects but it may also consider biomass and hydro...

  1. Boosting investor yields through bond insurance

    SciTech Connect (OSTI)

    Mosbacher, M.L.; Burkhardt, D.A.

    1993-02-01

    The market for utility securities generally tends to be fairly static. Innovative financing techniques are rarely used because of the marketability of utility securities stemming from the companies' generally strong financial credit and the monopoly markets most utilities serve. To many people, utility securities are considered the pillars of the financial world, and innovation is not needed. Further, plain vanilla utility issues are easily understood by investors, as well as by regulators and customers. Over the past several years, however, a new utility bond product has crept into the world of utility securities - insured secondary utility bonds. These insured bonds may possibly be used as an alternative financing technique for newly issued debt. Individual investors often tend to rely on insurance as a tool for reducing credit risk and are willing to take the lower yields as a tradeoff. Insured utility bonds are created by brokerage firms through the acqusition of a portion of an outstanding utility bond issue and subsequent solicitation of the insurance companies for bids. The insurance company then agrees to insure that portion of the issue until maturity for a fee, and the brokerage firm sells those bonds to their customers as a AAA-insured bond. Issuers are encouraged to explore the retail market as a financing alternative. They may find a most cost-effective means of raising capital.

  2. Clean Energy Investment Center Holds its Inaugural Laboratory-Investor

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Knowledge Seminar in Silicon Valley | Department of Energy Holds its Inaugural Laboratory-Investor Knowledge Seminar in Silicon Valley Clean Energy Investment Center Holds its Inaugural Laboratory-Investor Knowledge Seminar in Silicon Valley May 3, 2016 - 3:39pm Addthis Success! The Clean Energy Investment Center (CEIC) held its inaugural Laboratory-Investor Knowledge Seminar (LINKS) on Thursday, April 28, co-hosted with Lawrence Livermore National Laboratory at Innovate Pleasanton in

  3. New Financing Tool Expands Market to Institutional Investors

    Energy.gov [DOE]

    A new financing platform designed to open the market for energy efficiency to institutional investors, known as Warehouse for Energy Efficiency Loans (WHEEL), was launched in April 2014

  4. Investor and entrepreneur visits Los Alamos for open coffee

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    is a nationally recognized speaker on the topics of venture capital investing and entrepreneurship. Open Coffee Club encourages entrepreneurs, developers, and investors to organize...

  5. Investor Deal Summary Template (IDIQ Attachment J-11)

    Energy.gov [DOE]

    Document features a template for summarizing an investor or energy service company's (ESCO) deal as part of an energy savings performance contract (ESPC).

  6. NREL Forum Attracts Clean Energy Investors and Entrepreneurs...

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Forum Attracts Clean Energy Investors and Entrepreneurs Industry Growth Forum to feature business presentations from 30 clean energy startups, compelling panels and speakers, ...

  7. Investor Flows and the 2008 Boom/Bust in Oil Prices

    Gasoline and Diesel Fuel Update

    Introduction Investor Flows and Speculation New Evidence on Investor Flows and Oil Prices References Investor Flows and the 2008 Boom/Bust in Oil Prices Kenneth J. Singleton Graduate School of Business Stanford University August, 2011 Introduction Investor Flows and Speculation New Evidence on Investor Flows and Oil Prices References Investor Flows, Speculation, and Oil Prices The role of speculation (broadly construed) in the dramatic rise and subsequent sharp decline in oil prices during 2008?

  8. New Financing Tool Expands Market to Institutional Investors...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    New Financing Tool Expands Market to Institutional Investors Photo of the wheel and helm of a boat. A new financing platform designed to open the market for energy efficiency to ...

  9. Clean Energy Inventions and Achieving Scale; CEIC Engages Future Investors

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    of the World at the Duke Fuqua School of Business EDGE Seminar | Department of Energy Inventions and Achieving Scale; CEIC Engages Future Investors of the World at the Duke Fuqua School of Business EDGE Seminar Clean Energy Inventions and Achieving Scale; CEIC Engages Future Investors of the World at the Duke Fuqua School of Business EDGE Seminar September 26, 2016 - 5:01pm Addthis CEIC Director Sanjiv Malhotra speaking at Duke University's EDGE Seminar (credit CEIC/OTT). CEIC Director

  10. NREL's Industry Growth Forum Attracts Clean Energy Investors - News

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Releases | NREL NREL's Industry Growth Forum Attracts Clean Energy Investors 22nd Forum to Feature 34 Clean Energy Companies October 9, 2008 Thirty-four clean energy companies will present their business cases to a panel of investors and industry experts in Denver Nov. 3-5 as the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) hosts the 22nd NREL Industry Growth Forum. The 34 companies were selected through an application and review process and will compete for the

  11. NREL's Industry Growth Forum Attracts Clean Energy Investors - News

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Releases | NREL NREL's Industry Growth Forum Attracts Clean Energy Investors 25th Forum to Feature 30 Clean Energy Companies September 17, 2012 Thirty clean energy companies will present their business cases to a panel of investors and industry experts in Denver Oct. 23-24 as the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) hosts the 25th NREL Industry Growth Forum. The 30 companies were selected through an application and review process and will compete for the

  12. Measurement of the direct <mi>CP> -violating parameter <mi>Ami><mi>CP> in the decay <mi>D>+<mi>Kmi>-<mimi>+<mi>π>+

    SciTech Connect (OSTI)

    Abazov, V. M.; Abbott, B.; Acharya, B. S.; Adams, M.; Adams, T.; Agnew, J. P.; Alexeev, G. D.; Alkhazov, G.; Alton, A.; Askew, A.; Atkins, S.; Augsten, K.; Avila, C.; Badaud, F.; Bagby, L.; Baldin, B.; Bandurin, D. V.; Banerjee, S.; Barberis, E.; Baringer, P.; Bartlett, J. F.; Bassler, U.; Bazterra, V.; Bean, A.; Begalli, M.; Bellantoni, L.; Beri, S. B.; Bernardi, G.; Bernhard, R.; Bertram, I.; Besançon, M.; Beuselinck, R.; Bhat, P. C.; Bhatia, S.; Bhatnagar, V.; Blazey, G.; Blessing, S.; Bloom, K.; Boehnlein, A.; Boline, D.; Boos, E. E.; Borissov, G.; Borysova, M.; Brandt, A.; Brandt, O.; Brock, R.; Bross, A.; Brown, D.; Bu, X. B.; Buehler, M.; Buescher, V.; Bunichev, V.; Burdin, S.; Buszello, C. P.; Camacho-Pérez, E.; Casey, B. C. K.; Castilla-Valdez, H.; Caughron, S.; Chakrabarti, S.; Chan, K. M.; Chandra, A.; Chapon, E.; Chen, G.; Cho, S. W.; Choi, S.; Choudhary, B.; Cihangir, S.; Claes, D.; Clutter, J.; Cooke, M.; Cooper, W. E.; Corcoran, M.; Couderc, F.; Cousinou, M. -C.; Cutts, D.; Das, A.; Davies, G.; de Jong, S. J.; De La Cruz-Burelo, E.; Déliot, F.; Demina, R.; Denisov, D.; Denisov, S. P.; Desai, S.; Deterre, C.; DeVaughan, K.; Diehl, H. T.; Diesburg, M.; Ding, P. F.; Dominguez, A.; Dubey, A.; Dudko, L. V.; Duperrin, A.; Dutt, S.; Eads, M.; Edmunds, D.; Ellison, J.; Elvira, V. D.; Enari, Y.; Evans, H.; Evdokimov, V. N.; Fauré, A.; Feng, L.; Ferbel, T.; Fiedler, F.; Filthaut, F.; Fisher, W.; Fisk, H. E.; Fortner, M.; Fox, H.; Fuess, S.; Garbincius, P. H.; Garcia-Bellido, A.; García-González, J. A.; Gavrilov, V.; Geng, W.; Gerber, C. E.; Gershtein, Y.; Ginther, G.; Gogota, O.; Golovanov, G.; Grannis, P. D.; Greder, S.; Greenlee, H.; Grenier, G.; Gris, Ph.; Grivaz, J. -F.; Grohsjean, A.; Grünendahl, S.; Grünewald, M. W.; Guillemin, T.; Gutierrez, G.; Gutierrez, P.; Haley, J.; Han, L.; Harder, K.; Harel, A.; Hauptman, J. M.; Hays, J.; Head, T.; Hebbeker, T.; Hedin, D.; Hegab, H.; Heinson, A. P.; Heintz, U.; Hensel, C.; Heredia-De La Cruz, I.; Herner, K.; Hesketh, G.; Hildreth, M. D.; Hirosky, R.; Hoang, T.; Hobbs, J. D.; Hoeneisen, B.; Hogan, J.; Hohlfeld, M.; Holzbauer, J. L.; Howley, I.; Hubacek, Z.; Hynek, V.; Iashvili, I.; Ilchenko, Y.; Illingworth, R.; Ito, A. S.; Jabeen, S.; Jaffré, M.; Jayasinghe, A.; Jeong, M. S.; Jesik, R.; Jiang, P.; Johns, K.; Johnson, E.; Johnson, M.; Jonckheere, A.; Jonsson, P.; Joshi, J.; Jung, A. W.; Juste, A.; Kajfasz, E.; Karmanov, D.; Katsanos, I.; Kaur, M.; Kehoe, R.; Kermiche, S.; Khalatyan, N.; Khanov, A.; Kharchilava, A.; Kharzheev, Y. N.; Kiselevich, I.; Kohli, J. M.; Kozelov, A. V.; Kraus, J.; Kumar, A.; Kupco, A.; Kurča, T.; Kuzmin, V. A.; Lammers, S.; Lebrun, P.; Lee, H. S.; Lee, S. W.; Lee, W. M.; Lei, X.; Lellouch, J.; Li, D.; Li, H.; Li, L.; Li, Q. Z.; Lim, J. K.; Lincoln, D.; Linnemann, J.; Lipaev, V. V.; Lipton, R.; Liu, H.; Liu, Y.; Lobodenko, A.; Lokajicek, M.; Lopes de Sa, R.; Luna-Garcia, R.; Lyon, A. L.; Maciel, A. K. A.; Madar, R.; Magaña-Villalba, R.; Malik, S.; Malyshev, V. L.; Mansour, J.; Martínez-Ortega, J.; McCarthy, R.; McGivern, C. L.; Meijer, M. M.; Melnitchouk, A.; Menezes, D.; Mercadante, P. G.; Merkin, M.; Meyer, A.; Meyer, J.; Miconi, F.; Mondal, N. K.; Mulhearn, M.; Nagy, E.; Narain, M.; Nayyar, R.; Neal, H. A.; Negret, J. P.; Neustroev, P.; Nguyen, H. T.; Nunnemann, T.; Orduna, J.; Osman, N.; Osta, J.; Pal, A.; Parashar, N.; Parihar, V.; Park, S. K.; Partridge, R.; Parua, N.; Patwa, A.; Penning, B.; Perfilov, M.; Peters, Y.; Petridis, K.; Petrillo, G.; Pétroff, P.; Pleier, M. -A.; Podstavkov, V. M.; Popov, A. V.; Prewitt, M.; Price, D.; Prokopenko, N.; Qian, J.; Quadt, A.; Quinn, B.; Ratoff, P. N.; Razumov, I.; Ripp-Baudot, I.; Rizatdinova, F.; Rominsky, M.; Ross, A.; Royon, C.; Rubinov, P.; Ruchti, R.; Sajot, G.; Sánchez-Hernández, A.; Sanders, M. P.; Santos, A. S.; Savage, G.; Savitskyi, M.; Sawyer, L.; Scanlon, T.; Schamberger, R. D.; Scheglov, Y.; Schellman, H.; Schwanenberger, C.; Schwienhorst, R.; Sekaric, J.; Severini, H.; Shabalina, E.; Shary, V.; Shaw, S.; Shchukin, A. A.; Simak, V.; Skubic, P.; Slattery, P.; Smirnov, D.; Snow, G. R.; Snow, J.; Snyder, S.; Söldner-Rembold, S.; Sonnenschein, L.; Soustruznik, K.; Stark, J.; Stoyanova, D. A.; Strauss, M.; Suter, L.; Svoisky, P.; Titov, M.; Tokmenin, V. V.; Tsai, Y. -T.; Tsybychev, D.; Tuchming, B.; Tully, C.; Uvarov, L.; Uvarov, S.; Uzunyan, S.; Van Kooten, R.; van Leeuwen, W. M.; Varelas, N.; Varnes, E. W.; Vasilyev, I. A.; Verkheev, A. Y.; Vertogradov, L. S.; Verzocchi, M.; Vesterinen, M.; Vilanova, D.; Vokac, P.; Wahl, H. D.; Wang, M. H. L. S.; Warchol, J.; Watts, G.; Wayne, M.; Weichert, J.; Welty-Rieger, L.; Williams, M. R. J.; Wilson, G. W.; Wobisch, M.; Wood, D. R.; Wyatt, T. R.; Xie, Y.; Yamada, R.; Yang, S.; Yasuda, T.; Yatsunenko, Y. A.; Ye, W.; Ye, Z.; Yin, H.; Yip, K.; Youn, S. W.; Yu, J. M.; Zennamo, J.; Zhao, T. G.; Zhou, B.; Zhu, J.; Zielinski, M.; Zieminska, D.; Zivkovic, L.

    2014-12-01

    We measure the direct mi>Cmi>mi>P>-violating parameter mi>Ami>mi>Cmi>mi>Pmi> for the decay of the charged charm meson, mi>Dmi>+mi>Kmi>-mi>πmi>+mi>πmi>+ (and charge conjugate), using the full 10.4 mi>fbmi>-1 sample of mi>p>mi>p>¯ collisions at mi>smi>=1.96 mi>TeVmi> collected by the D0 detector at the Fermilab Tevatron collider. We extract the raw reconstructed charge asymmetry by fitting the invariant mass distributions for the sum and difference of charge-specific samples. This quantity is then corrected for detector-related asymmetries using data-driven methods and for possible physics asymmetries (from mi>B>mi>D

  13. Bring Your Own Device

    Office of Energy Efficiency and Renewable Energy (EERE)

    Bring your own Device, or BYOD, has been a popular topic for some time now. While government organizations and private companies continue to struggle with how to enjoy the business and economic...

  14. Investor Flows and the 2008 Boom/Bust in Oil Prices

    Annual Energy Outlook

    ... MMSPD13: the thirteen-week change in managed-money spread positions in millions, as constructed by the CFTC. Introduction Investor Flows and Speculation New Evidence on Investor ...

  15. COP 21 Climate Summit's Momentum Keeps Going at the 2016 Investor Summit

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    | Department of Energy COP 21 Climate Summit's Momentum Keeps Going at the 2016 Investor Summit COP 21 Climate Summit's Momentum Keeps Going at the 2016 Investor Summit February 1, 2016 - 2:45pm Addthis The 2016 Investor Summit on Climate Risk was the first major event on climate change for investors and businesses to discuss the far-reaching implications of the Paris Agreement. Ceres, the United Nations Foundation, and the United Nations Office for Partnerships co-hosted the Summit at the

  16. Energy Deputy Secretary Poneman Tours Minority-Owned Small Business in

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Detroit | Department of Energy Tours Minority-Owned Small Business in Detroit Energy Deputy Secretary Poneman Tours Minority-Owned Small Business in Detroit May 22, 2012 - 10:37am Addthis Deputy Secretary of Energy Daniel Poneman meets with owners and workers at Diversified Chemical Technologies, a small business in Detroit, MI. | Energy Department photo Deputy Secretary of Energy Daniel Poneman meets with owners and workers at Diversified Chemical Technologies, a small business in Detroit,

  17. OTT's Clean Energy Investment Center Holds its Inaugural Laboratory-Investor Knowledge Seminar in Silicon Valley

    Energy.gov [DOE]

    The Clean Energy Investment Center (CEIC) held its inaugural Laboratory-Investor Knowledge Seminar (LINKS) on Thursday, April 28, co-hosted with Lawrence Livermore National Laboratory at Innovate Pleasanton in Pleasanton, CA.

  18. US ENC MI Site Consumption

    U.S. Energy Information Administration (EIA) (indexed site)

    MI Site Consumption million Btu $0 $500 $1,000 $1,500 $2,000 $2,500 US ENC MI Expenditures dollars ALL ENERGY average per household (excl. transportation) 0 2,000 4,000 6,000 8,000 10,000 12,000 US ENC MI Site Consumption kilowatthours $0 $250 $500 $750 $1,000 $1,250 $1,500 US ENC MI Expenditures dollars ELECTRICITY ONLY average per household * Michigan households use 123 million Btu of energy per home, 38% more than the U.S. average. * High consumption, combined with low costs for heating fuels

  19. US ENC MI Site Consumption

    Annual Energy Outlook

    ... Yes Yes No No 0% 20% 40% 60% 80% 100% US MI No Car CAR IS PARKED WITHIN 20 FT OF ELECTRICAL OUTLET More highlights from RECS on housing characteristics and energy-related ...

  20. NREL's 23rd Industry Growth Forum Attracts Clean Energy Investors - News

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Releases | NREL NREL's 23rd Industry Growth Forum Attracts Clean Energy Investors Forum to Feature 34 Clean Energy Companies September 23, 2010 Thirty-four clean energy companies will present their business cases to a panel of investors and industry experts in Denver Oct. 19-21 as the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) hosts the 23rd NREL Industry Growth Forum. The 34 companies were selected through an application and review process and will compete for

  1. Six ARPA-E Projects Illustrate Private Investors Excited About Clean Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Innovation | Department of Energy Six ARPA-E Projects Illustrate Private Investors Excited About Clean Energy Innovation Six ARPA-E Projects Illustrate Private Investors Excited About Clean Energy Innovation February 3, 2011 - 12:00am Addthis Washington, DC - In a little over one year, six projects that received a total of $23.6 million in seed funding from the Department of Energy's Advanced Research Project Agency - Energy (ARPA-E) have generated more than $100 million in outside private

  2. Are neutrinos their own antiparticles?

    SciTech Connect (OSTI)

    Kayser, Boris; /Fermilab

    2009-03-01

    We explain the relationship between Majorana neutrinos, which are their own antiparticles, and Majorana neutrino masses. We point out that Majorana masses would make the neutrinos very distinctive particles, and explain why many theorists strongly suspect that neutrinos do have Majorana masses. The promising approach to confirming this suspicion is to seek neutrinoless double beta decay. We introduce a toy model that illustrates why this decay requires nonzero neutrino masses, even when there are both right-handed and left-handed weak currents.

  3. Real-time sub-<mi>>ngstrom...

    Office of Scientific and Technical Information (OSTI)

    Real-time sub-<mi>>ngstrom imaging of reversible and irreversible conformations in rhodium catalysts and graphene Kisielowski, Christian; Wang,...

  4. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    West Virginia" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",5,2,"NA",2,"NA","NA","NA"," " "Number of retail

  5. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Alaska" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",17,34,"NA",19,"NA","NA","NA"," " "Number of retail

  6. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    District of Columbia" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",3,"NA","NA","NA","NA",26,1," " "Number of retail

  7. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Hawaii" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",14,"NA","NA",1,2,"NA","NA"," " "Number of retail

  8. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Indiana" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",7,72,"NA",39,"NA","NA","NA"," " "Number of retail

  9. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Iowa" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",3,137,"NA",42,"NA","NA","NA"," " "Number of retail

  10. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Louisiana" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",7,22,"NA",12,"NA","NA","NA"," " "Number of retail

  11. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Missouri" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",5,86,"NA",42,"NA","NA","NA"," " "Number of retail

  12. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Nebraska" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities","NA",148,1,10,"NA","NA","NA"," " "Number of retail

  13. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Carolina" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",4,22,"NA",21,"NA","NA","NA"," " "Number of retail

  14. Mi GmbH | Open Energy Information

    Open Energy Information (Open El) [EERE & EIA]

    Mi GmbH Jump to: navigation, search Name: Mi GmbH Place: Switzerland Zip: CH-6340 Sector: Solar Product: Baar-based manufacturer and distributor of fruit juices. The firm is also...

  15. Who Owns Renewable Energy Certificates?

    SciTech Connect (OSTI)

    Holt, Edward; Wiser, Ryan; Bolinger, Mark

    2006-06-01

    Renewable energy certificates (RECs) are tradable instruments that convey the attributes of a renewable energy generator and the right to make certain claims about energy purchases. RECs first appeared in US markets in the late 1990s and are particularly important in states that accept or require them as evidence of compliance with renewables portfolio standards (RPS). The emergence of RECs as a tradable commodity has made utilities, generators, and regulators increasingly aware of the need to specify who owns the RECs in energy transactions. In voluntary transactions, most agree that the question of REC ownership can and should be negotiated privately between the buyer and the seller, and should be clearly established by contract. Claims about purchasing or using renewable energy should only be made if REC ownership can be documented. In many other cases, however, renewable energy transactions are either mandated or encouraged through state or federal policy. Because of the recent appearance of RECs, legislation and regulation mandating the purchase of renewable energy has sometimes been silent on the disposition of the RECs associated with that generation. Furthermore, some renewable energy contracts pre-date the existence of RECs, and therefore do not address REC ownership. In both of these instances, the issue of REC ownership must often be answered by legislative or regulatory authorities. The resulting uncertainty in REC ownership has hindered the development of robust REC markets and has, in some cases, led to contention between buyers and sellers of renewable generation. This article, which is based on a longer Berkeley Lab report, reviews federal and state efforts to clarify the ownership of RECs from Qualifying Facilities (QFs) that sell their generation under the Public Utility Regulatory Policies Act (PURPA) of 1978. The full report also addresses state efforts to clarify REC ownership in two other situations, customer-owned generation that benefits

  16. Abating climate change. What will be done and the consequences for investors

    SciTech Connect (OSTI)

    Raskin, A.; Mellquist, N.; Shah, S.; Winton, B.

    2008-01-15

    This report is meant for institutional investors. Sections discuss: the science of climate change; global emissions - abatement scenario; coal - the world's dominant fuel for electric generation; CO{sub 2} transport and storage; nuclear energy - the new green solution; renewable energy - harnessing the power of water, wind and sun; regulation; increasing energy efficiency - the lowest cost option; enhancing the efficiency of electric applications; enhancing the efficiency of transportation; macroeconomic implications; and investment implications.

  17. Buildings*","Nongovernment-Owned Buildings",,,,"Government-Owned Buildings"

    U.S. Energy Information Administration (EIA) (indexed site)

    8. Occupancy of Nongovernment-Owned and Government-Owned Buildings, Floorspace for Non-Mall Buildings, 2003" ,"Total Floorspace (million square feet)" ,"All Buildings*","Nongovernment-Owned Buildings",,,,"Government-Owned Buildings" ,,"Nongov- ernment- Owned Buildings","Owner Occupied","Nonowner Occupied","Unocc- upied","Govern- ment- Owned

  18. miRNAs in brain development

    SciTech Connect (OSTI)

    Petri, Rebecca; Malmevik, Josephine; Fasching, Liana; Åkerblom, Malin; Jakobsson, Johan

    2014-02-01

    MicroRNAs (miRNAs) are small, non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. In the brain, a large number of miRNAs are expressed and there is a growing body of evidence demonstrating that miRNAs are essential for brain development and neuronal function. Conditional knockout studies of the core components in the miRNA biogenesis pathway, such as Dicer and DGCR8, have demonstrated a crucial role for miRNAs during the development of the central nervous system. Furthermore, mice deleted for specific miRNAs and miRNA-clusters demonstrate diverse functional roles for different miRNAs during the development of different brain structures. miRNAs have been proposed to regulate cellular functions such as differentiation, proliferation and fate-determination of neural progenitors. In this review we summarise the findings from recent studies that highlight the importance of miRNAs in brain development with a focus on the mouse model. We also discuss the technical limitations of current miRNA studies that still limit our understanding of this family of non-coding RNAs and propose the use of novel and refined technologies that are needed in order to fully determine the impact of specific miRNAs in brain development. - Highlights: • miRNAs are essential for brain development and neuronal function. • KO of Dicer is embryonically lethal. • Conditional Dicer KO results in defective proliferation or increased apoptosis. • KO of individual miRNAs or miRNA families is necessary to determine function.

  19. ARM - Lesson Plans: Your Own Greenhouse

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Your Own Greenhouse Outreach Home Room News Publications Traditional Knowledge Kiosks Barrow, Alaska Tropical Western Pacific Site Tours Contacts Students Study Hall About ARM Global Warming FAQ Just for Fun Meet our Friends Cool Sites Teachers Teachers' Toolbox Lesson Plans Lesson Plans: Your Own Greenhouse Objective The objective is to make your own small greenhouse and in a simple way to test its effect on temperature. Materials Each student or group of students will need the following:

  20. DOE - Office of Legacy Management -- Detrex Corp - MI 10

    Office of Legacy Management (LM)

    Detroit , Michigan MI.10-1 Evaluation Year: 1987 MI.10-2 Site Operations: Conducted experimental runs relative to picklingdegreasing of one handful of uranium turnings MI.10-1...

  1. DOE - Office of Legacy Management -- Adrian - MI 01

    Office of Legacy Management (LM)

    Adrian - MI 01 FUSRAP Considered Sites Adrian, MI Alternate Name(s): Bridgeport Brass Co. Special Metals Extrusion Plant Bridgeport Brass Company General Motors General Motors Company, Adrian MI.01-1 Location: 1450 East Beecher Street, Adrian, Michigan MI.01-3 Historical Operations: Performed uranium extrusion research and development and metal fabrication work for the AEC using uranium, thorium, and plutonium. MI.01-2 Eligibility Determination: Eligible MI.01-1 Radiological Survey(s):

  2. DOE - Office of Legacy Management -- Oliver Corp - MI 11

    Office of Legacy Management (LM)

    OLIVER CORP. (MI.11 ) Eliminated from further consideration under FUSRAP - Referred to NRC Designated Name: Not Designated Alternate Name: Behnke Warehousing Incorporated MI.11-1...

  3. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Vermont" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",2,14,"NA",2,1,"NA","NA"," " "Number of retail customers",258928,54912,"NA",49378,1,"NA","NA",363219 "Retail

  4. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Virginia" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",3,16,"NA",13,"NA",1,1," " "Number of retail customers",2934456,166751,"NA",629034,"NA",20,"NA",3730261 "Retail sales

  5. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Washington" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",4,41,2,18,1,3,1," " "Number of retail customers",1460672,1669068,10,167371,1,17,"NA",3297139 "Retail sales

  6. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Wisconsin" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",13,82,"NA",24,2,"NA","NA"," " "Number of retail customers",2439647,282258,"NA",260892,2,"NA","NA",2982799

  7. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Wyoming" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",5,13,1,18,"NA","NA","NA"," " "Number of retail customers",198292,36318,5,99606,"NA","NA","NA",334221 "Retail

  8. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    United States" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",256,1948,6,810,144,188,67," " "Number of retail customers",93329397,21335809,40029,19096482,656,13411030,"NA",147213403 "Retail sales

  9. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Arizona" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",23,29,3,9,11,"NA","NA"," " "Number of retail customers",1675038,1078638,16690,187629,12,"NA","NA",2958007 "Retail sales

  10. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    California" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",29,41,2,4,65,17,3," " "Number of retail customers",11676056,3110257,2197,16506,69,185755,"NA",14990840 "Retail sales

  11. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Colorado" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",10,29,1,28,7,"NA","NA"," " "Number of retail customers",1500660,428854,13,632335,7,"NA","NA",2561869 "Retail sales

  12. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Connecticut" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",7,8,"NA","NA",3,35,2," " "Number of retail customers",948486,71741,"NA","NA",3,597272,"NA",1617502 "Retail sales

  13. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Delaware" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",2,9,"NA",1,1,27,1," " "Number of retail customers",267434,66283,"NA",88026,1,38537,"NA",460281 "Retail sales

  14. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Georgia" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",2,53,"NA",42,1,"NA","NA"," " "Number of retail customers",2410042,333203,"NA",1966788,31,"NA","NA",4710064

  15. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Idaho" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",3,11,2,17,"NA","NA","NA"," " "Number of retail customers",693393,43895,1,84578,"NA","NA","NA",821867 "Retail

  16. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Illinois" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",4,41,"NA",26,2,53,3," " "Number of retail customers",1911129,270483,"NA",301219,318,3268220,"NA",5751369 "Retail sales

  17. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Kansas" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",4,118,1,29,"NA","NA","NA"," " "Number of retail customers",953679,235288,4,292717,"NA","NA","NA",1481688 "Retail

  18. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Kentucky" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",5,30,1,24,2,"NA","NA"," " "Number of retail customers",1220619,210206,17,813201,4,"NA","NA",2244047 "Retail sales

  19. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Maine" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",2,4,"NA",2,1,32,6," " "Number of retail customers",39,10603,"NA",2535,1,788335,"NA",801513 "Retail sales

  20. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Maryland" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",10,5,"NA",3,8,52,5," " "Number of retail customers",1638979,28808,"NA",208447,8,610640,"NA",2486882 "Retail sales

  1. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Massachusetts" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",13,40,"NA","NA",27,40,5," " "Number of retail customers",2182382,399857,"NA","NA",40,544399,"NA",3126678 "Retail

  2. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Michigan" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",8,41,"NA",10,2,12,3," " "Number of retail customers",4177118,306315,"NA",318985,2,6419,"NA",4808839 "Retail sales

  3. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Mississippi" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",2,23,1,25,"NA","NA","NA"," " "Number of retail customers",628656,134500,7,741758,"NA","NA","NA",1504921

  4. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Montana" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",5,1,3,29,"NA",2,1," " "Number of retail customers",377770,983,20971,197627,"NA",419,"NA",597770 "Retail sales

  5. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Hampshire" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",3,5,"NA",1,"NA",20,4," " "Number of retail customers",496060,12226,"NA",78794,"NA",128985,"NA",716065 "Retail sales

  6. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Jersey" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",17,9,"NA",1,35,58,4," " "Number of retail customers",3270179,55120,"NA",11581,39,649669,"NA",3986588 "Retail sales

  7. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    York" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",19,48,"NA",4,5,69,9," " "Number of retail customers",5052054,1270394,"NA",18139,15,1751992,"NA",8092594 "Retail sales

  8. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Dakota" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",3,12,1,21,"NA","NA","NA"," " "Number of retail customers",238608,11023,21,186997,"NA","NA","NA",436649 "Retail

  9. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Ohio" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",10,85,"NA",25,6,52,6," " "Number of retail customers",2143362,375117,"NA",383167,12,2618989,"NA",5520647 "Retail sales

  10. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Oklahoma" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",3,62,1,31,"NA","NA","NA"," " "Number of retail customers",1291253,204450,1,508162,"NA","NA","NA",2003866

  11. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Oregon" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",8,18,1,19,"NA",4,3," " "Number of retail customers",1421279,294747,1,203211,"NA",484,"NA",1919722 "Retail sales

  12. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Pennsylvania" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",15,35,"NA",13,5,73,10," " "Number of retail customers",3554206,83922,"NA",219570,5,2146096,"NA",6003799 "Retail sales

  13. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Rhode Island" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",2,1,"NA","NA","NA",17,1," " "Number of retail customers",462381,4658,"NA","NA","NA",32071,"NA",499110

  14. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Dakota" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",6,36,1,31,"NA","NA","NA"," " "Number of retail customers",243148,60553,22,154530,"NA","NA","NA",458253 "Retail

  15. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Tennessee" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",3,61,1,26,1,"NA","NA"," " "Number of retail customers",47264,2213496,23,969214,1,"NA","NA",3229998 "Retail sales

  16. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Texas" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",89,72,"NA",68,13,"NA","NA"," " "Number of retail customers",7744205,1849743,"NA",2076859,50,"NA","NA",11670857

  17. Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total"

    U.S. Energy Information Administration (EIA) (indexed site)

    Utah" ,"Full service providers",,,,,"Other providers",, "Item","Investor Owned","Public","Federal","Cooperative","Non-utility","Energy","Delivery","Total" "Number of entities",1,40,1,9,1,"NA","NA"," " "Number of retail customers",835233,244217,7,48538,1,"NA","NA",1127996 "Retail sales

  18. Energy use in state owned facilities

    SciTech Connect (OSTI)

    Not Available

    1988-12-01

    This report contains the energy use data for Wisconsin's 32 largest state-owned government facilities. These facilities account for the majority of both fuels consumed in Wisconsin state buildings and total state-owned gross square footage. Figures for each agency reflect buildings owned but not necessarily occupied by that agency. Information is presented for each of the sixteen years from and including the base year, fiscal 1972--1973 (chosen because it was the last year before the oil embargo). Agencies addressed in this report are the Department of Public Instruction (DPI), the University of Wisconsin System (UWS), the Department of Health and Social Services (DHSS), the Department of Veterans Affairs (DVA), and the Department of Administration (DOA). 16 figs., 40 tabs.

  19. The NuMI neutrino beam

    DOE PAGES-Beta [OSTI]

    Adamson, P.; Anderson, K.; Andrews, M.; Andrews, R.; Anghel, I.; Augustine, D.; Aurisano, A.; Avvakumov, S.; Ayres, D. S.; Baller, B.; et al

    2015-10-20

    Our paper describes the hardware and operations of the Neutrinos at the Main Injector (NuMI) beam at Fermilab. It elaborates on the design considerations for the beam as a whole and for individual elements. The most important part of our design details pertaining to individual components is described. Beam monitoring systems and procedures, including the tuning and alignment of the beam and NuMI long-term performance, are also discussed.

  20. The NuMI Neutrino Beam

    SciTech Connect (OSTI)

    Adamson, P.; Anderson, K.; Andrews, M.; Andrews, R.; Anghel, I.; Augustine, D.; Aurisano, A.; Avvakumov, S.; Ayres, D. S.; Baller, B.; Barish, B.; Barr, G.; Barrett, W. L.; Berstein, R. H.; Biggs, J.; Bishai, M.; Blake, A.; Bocean, V.; Bock, G. J.; Boehnlein, D. J.; Bogert, D.; Bourkland, K.; Cao, S. V.; Castromonte, C. M.; Childress, S.; Choudhary, B. C.; Coelho, J. A.B.; Cobb, J. H.; Corwin, L.; Crane, D.; Cravens, J. P.; Cronin-Hennessy, D.; Ducar, R. J.; De Jong, J. K.; Devan, A. V.; Devenish, N. E.; Diwan, M. V.; Erwin, A. R.; Escobar, C. O.; Evans, J. J.; Falk, E.; Feldman, G. J.; Fields, T. H.; Ford, R.; Frohne, M. V.; Gallahger, H. R.; Garkusha, V.; Gomes, R. A.; Goodman, M. C.; Gouffon, P.; Graf, N.; Gran, R.; Grossman, N.; Grzelak, K.; Habig, A.; Hahn, S. R.; Harding, D.; Harris, D.; Harris, P. G.; Hartnell, J.; Hatcher, R.; Hays, S.; Heller, K.; Holin, A.; Huang, J.; Hylen, J.; Ibrahim, A.; Indurthy, D.; Irwin, G. M.; Isvan, Z.; Jaffe, D. E.; James, C.; Jensen, D.; Johnstone, J.; Kafka, T.; Kasahara, S. M.S.; Koizumi, G.; Kopp, S.; Kordosky, M.; Kreymer, A.; Lang, K.; Laughton, C.; Lefeuvre, G.; Ling, J.; Litchfield, P. J.; Loiacono, L.; Lucas, P.; Mann, W. A.; Marchionni, A.; Marshak, M. L.; Mayer, N.; McGivern, C.; Medeiros, M. M.; Mehdiyev, R.; Meier, J. R.; Messier, M. D.; Michael, D. G.; Milburn, R. H.; Miller, J. L.; Miller, W. H.; Mishra, S. R.; Sher, S. Moed; Moore, C. D.; Morfin, J.; Mualem, L.; Mufson, S.; Murgia, S.; Murtagh, M.; Musser, J.; Naples, D.; Nelson, J. K.; Newman, H. B.; Nichol, R. J.; Nowak, J. A.; O'connor, J.; Oliver, W. P.; Olsen, M.; Orchanian, M.; Osprey, S.; Pahlka, R. B.; Paley, J.; Para, A.; Patterson, R. B.; Patzak, T.; Pavlovic, Z.; Pawloski, G.; Perch, A.; Peterson, E. A.; Petyt, D. A.; Pfutzner, M. M.; Phan-Budd, S.; Plunkett, R. K.; Poonthottathil, N.; Prieto, P.; Pushka, D.; Qiu, X.; Radovic, A.; Rameika, R. A.; Ratchford, J.; Rebel, B.; Reilly, R.; Rosenfeld, C.; Rubin, H. A.; Ruddick, K.; Sanchez, M. C.; Saoulidou, N.; Sauer, L.; Schneps, J.; Schoo, D.; Schreckenberger, A.; Schreiner, P.; Shanahan, P.; Sharma, R.; Smart, W.; Smith, C.; Sousa, A.; Stefanik, A.; Tagg, N.; Talaga, R. L.; Tassotto, G.; Thomas, J.; Thompson, J.; Thomson, M. A.; Tian, X.; Timmons, A.; Tinsley, D.; Tognini, S. C.; Toner, R.; Torretta, D.; Trostin, I.; Tzanakos, G.; Urheim, J.; Vahle, P.; Vaziri, K.; Villegas, E.; Viren, B.; Vogel, G.; Webber, R. C.; Weber, A.; Webb, R. C.; Wehmann, A.; White, C.; Whitehead, L.; Whitehead, L. H.; Wojcicki, S. G.; Wong-Squires, M. L.; Yang, T.; Yumiceva, F. X.; Zarucheisky, V.; Zwaska, R.

    2015-10-20

    Our paper describes the hardware and operations of the Neutrinos at the Main Injector (NuMI) beam at Fermilab. It elaborates on the design considerations for the beam as a whole and for individual elements. The most important part of our design details pertaining to individual components is described. Beam monitoring systems and procedures, including the tuning and alignment of the beam and NuMI long-term performance, are also discussed.

  1. Performance Excellence Partners Wins Woman-Owned Small Business...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Performance Excellence Partners Wins Woman-Owned Small Business of the Year Award at Small Business Forum & Expo Performance Excellence Partners Wins Woman-Owned Small Business of ...

  2. DOE Awards Native American, Tribally-Owned Small Business Contract...

    Energy Savers

    Native American, Tribally-Owned Small Business Contract for Support Services to Savannah River Operations Office DOE Awards Native American, Tribally-Owned Small Business Contract ...

  3. Port Huron, MI Liquefied Natural Gas Exports to Canada (Million...

    U.S. Energy Information Administration (EIA) (indexed site)

    Huron, MI Liquefied Natural Gas Exports to Canada (Million Cubic Feet) Port Huron, MI Liquefied Natural Gas Exports to Canada (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul ...

  4. DOE - Office of Legacy Management -- Naval Ordnance Plant - MI...

    Office of Legacy Management (LM)

    Eliminated from further consideration under FUSRAP - Referred to DoD for action Designated ... MI.0-03-1 Site Disposition: Eliminated - No Authority - Referred to DoD MI.0-03-1 ...

  5. miR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor

    SciTech Connect (OSTI)

    Park, Jong-Kook; Henry, Jon C.; Jiang, Jinmai; Esau, Christine; Gusev, Yuriy; Lerner, Megan R.; Postier, Russell G.; Brackett, Daniel J.; Schmittgen, Thomas D.

    2011-03-25

    Research highlights: {yields} The expression of miR-132 and miR-212 are significantly increased in pancreatic cancer. {yields} miR-132 and miR-212 target the tumor suppressor pRb, resulting in enhanced proliferation. {yields} miR-132 and miR-212 expression is increased by a {beta}2 adrenergic receptor agonist, suggesting a novel mechanism for pancreatic cancer progression. -- Abstract: Numerous microRNAs (miRNAs) are reported as differentially expressed in cancer, however the consequence of miRNA deregulation in cancer is unknown for many miRNAs. We report that two miRNAs located on chromosome 17p13, miR-132 and miR-212, are over-expressed in pancreatic adenocarcinoma (PDAC) tissues. Both miRNAs are predicted to target the retinoblastoma tumor suppressor, Rb1. Validation of this interaction was confirmed by luciferase reporter assay and western blot in a pancreatic cancer cell line transfected with pre-miR-212 and pre-miR-132 oligos. Cell proliferation was enhanced in Panc-1 cells transfected with pre-miR-132/-212 oligos. Conversely, antisense oligos to miR-132/-212 reduced cell proliferation and caused a G{sub 2}/M cell cycle arrest. The mRNA of a number of E2F transcriptional targets were increased in cells over expressing miR-132/-212. Exposing Panc-1 cells to the {beta}2 adrenergic receptor agonist, terbutaline, increased the miR-132 and miR-212 expression by 2- to 4-fold. We report that over-expression of miR-132 and miR-212 result in reduced pRb protein in pancreatic cancer cells and that the increase in cell proliferation from over-expression of these miRNAs is likely due to increased expression of several E2F target genes. The {beta}2 adrenergic pathway may play an important role in this novel mechanism.

  6. “Nodal Gap” induced by the incommensurate diagonal spin density modulation in underdoped high- <mi>Tmi>c> superconductors

    SciTech Connect (OSTI)

    Zhou, Tao; Gao, Yi; Zhu, Jian -Xin

    2015-03-07

    Recently it was revealed that the whole Fermi surface is fully gapped for several families of underdoped cuprates. The existence of the finite energy gap along the <mi>d>-wave nodal lines (nodal gap) contrasts the common understanding of the <mi>d>-wave pairing symmetry, which challenges the present theories for the high-<mi>Tmi><mi>c>superconductors. Here we propose that the incommensurate diagonal spin-density-wave order can account for the above experimental observation. The Fermi surface and the local density of states are also studied. Our results are in good agreement with many important experiments in high-<mi>Tmi><mi>c>superconductors.

  7. Characterization of function and regulation of miR-24-1 and miR-31

    SciTech Connect (OSTI)

    Sun Fenyong; Wang Jiayi; Pan Qiuhui; Yu Yongchun; Zhang Yue; Wan Yang; Wang Ju; Li Xiaoyan; Hong An

    2009-03-13

    To date, numerous microRNAs (miRNAs) have been discovered. However, the function of these miRNAs is largely unknown. While our knowledge of miRNA post-transcriptional processing has greatly expanded in recent years, we have a limited understanding of the regulation and transcription of miRNA genes. In this study, we characterized two BMP-2 upregulated miRNAs, miR-24-1 and miR-31, in mesenchymal stem cells and showed their opposing function in controlling cellular proliferation, and adipogenesis. Furthermore, we are the first to identify and characterize mouse intronic miR-23b{approx}27b{approx}24-1 and intergenic miR-31 genes. Moreover, we found that pri-miR-23b, pri-miR-27b, and pri-miR-24-1 are transcribed independently and their expression profiles are unique when cells are treated with BMP-2, even though they are located closely together.

  8. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Eligibility: Investor-Owned Utility, Municipal Utilities, Cooperative Utilities Renewables Portfolio Standard Eligible Technologies: Eligibility: Investor-Owned Utility, Local...

  9. Rover on Mars now picks its own laser targets

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Rover on Mars now picks its own laser targets Rover on Mars now picks its own laser targets If you find yourself on Mars anytime soon, beware: there's a rover exploring its ...

  10. Office of River Protection Women-Owned Small Business Contractor...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Office of River Protection Women-Owned Small Business Contractor Receives One-Year Extension Office of River Protection Women-Owned Small Business Contractor Receives One-Year ...

  11. BNL Technical Services Awarded Service-Disabled Veteran-Owned...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    BNL Technical Services Awarded Service-Disabled Veteran-Owned Small Business of the Year BNL Technical Services Awarded Service-Disabled Veteran-Owned Small Business of the Year ...

  12. GC GUIDANCE ON BARTER TRANSACTIONS INVOLVING DOE-OWNED URANIUM...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    GUIDANCE ON BARTER TRANSACTIONS INVOLVING DOE-OWNED URANIUM GC GUIDANCE ON BARTER TRANSACTIONS INVOLVING DOE-OWNED URANIUM The Department of Energy has on a variety of occasions ...

  13. Financial statistics of major publicly owned electric utilities, 1991

    SciTech Connect (OSTI)

    Not Available

    1993-03-31

    The Financial Statistics of Major Publicly Owned Electric Utilities publication presents summary and detailed financial accounting data on the publicly owned electric utilities. The objective of the publication is to provide Federal and State governments, industry, and the general public with data that can be used for policymaking and decisionmaking purposes relating to publicly owned electric utility issues.

  14. Magnetocrystalline anisotropy in <mi>UMn>2<mi>Ge>2 and related Mn-based actinide ferromagnets

    SciTech Connect (OSTI)

    Parker, David S.; Ghimire, Nirmal; Singleton, John; Thompson, J. D.; Bauer, Eric D.; Baumbach, Ryan; Mandrus, David; Li, Ling; Singh, David J.

    2015-05-04

    We present magnetization isotherms in pulsed magnetic fields up to 62 Tesla, supported by first principles calculations, demonstrating a huge uniaxial magnetocrystalline anisotropy energy - approximately 20 MJ/m3 - in <mi>UMn>2<mi>Ge>2. This large anisotropy results from the extremely strong spin-orbit coupling affecting the uranium 5 f electrons, which in the calculations exhibit a substantial orbital moment exceeding 2 μB. Finally, we also find from theoretical calculations that a number of isostructural Mn-actinide compounds are expected to have similarly large anisotropy.

  15. DOE - Office of Legacy Management -- Star Cutter Corp - MI 15

    Office of Legacy Management (LM)

    Eliminated - Potential for contamination considered remote based on limited scope and quantity of materials handled MI.15-2 Radioactive Materials Handled: Yes Primary ...

  16. MINOS Experiment and NuMI Beam Home Page

    U.S. Department of Energy (DOE) - all webpages

    NuMI-MINOS Neutrino Logo NuMI Beamline and MINOS Experiment Neutrino Logo The MINOS Experiment and NuMI Beamline Fermilab Logo MINOS Experiment Links ◊ MINOS for the Public ◊ Scientific Results ◊ MINOS at Work ◊ NuMI at Work ◊ MINOS+ Experiment Fermilab Neutrino Links ◊ Neutrino FAQ ◊ MINOS Underground Areas at Fermilab ◊ PPD Intensity Frontier Dept Back to - - - ◊ Fermilab at Work ◊ Fermilab Home the MINOS Far Detector in the Soudan Mine MINOS collaborators assembling the

  17. The Appraisal Process: Be Your Own Advocate | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    The Appraisal Process: Be Your Own Advocate The Appraisal Process: Be Your Own Advocate The Appraisal Process: Be Your Own Advocate, a presentation of the U.S. Department of Energy's DOE Zero Energy Ready Home program. ZERH Appraisal Process (3.03 MB) More Documents & Publications DOE ZERH Webinar: Marketing and Sales Solutions for Zero Energy Ready Homes Zero Energy Ready Home Training Presentation Collective Impact for Zero Net Energy Homes

  18. Rover on Mars now picks its own laser targets

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Rover on Mars now picks its own laser targets Rover on Mars now picks its own laser targets If you find yourself on Mars anytime soon, beware: there's a rover exploring its surface, and it now has the ability to choose its own targets for its onboard laser-and even fire it autonomously. August 1, 2016 Rover on Mars now picks its own laser targets This selfie of NASA's Curiosity Mars rover shows the vehicle at a drilled sample site called "Okoruso," on the "Naukluft Plateau"

  19. SBA Expands Access to Contracting Opportunities for Women-Owned...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    on the Small Business Administration's website. Women-owned small businesses will have greater access to federal contracting opportunities as a result of changes included ...

  20. Financial statistics of major US publicly owned electric utilities 1993

    SciTech Connect (OSTI)

    Not Available

    1995-02-01

    The 1993 edition of the Financial Statistics of Major U.S. Publicly Owned Electric Utilities publication presents five years (1989 to 1993) of summary financial data and current year detailed financial data on the major publicly owned electric utilities. The objective of the publication is to provide Federal and State governments, industry, and the general public with current and historical data that can be used for policymaking and decision making purposes related to publicly owned electric utility issues. Generator and nongenerator summaries are presented in this publication. The primary source of publicly owned financial data is the Form EIA-412, the Annual Report of Public Electric Utilities, filed on a fiscal basis.

  1. DOE Awards Native American, Tribally-Owned Small Business Contract...

    Energy Savers

    Small Business Contract for Support Services to Savannah River Operations Office DOE Awards Native American, Tribally-Owned Small Business Contract for Support Services to ...

  2. Green Energy Options for Consumer-Owned Business

    SciTech Connect (OSTI)

    Co-opPlus of Western Massachusetts

    2006-05-01

    The goal of this project was to define, test, and prototype a replicable business model for consumer-owned cooperatives. The result is a replicable consumer-owned cooperative business model for the generation, interconnection, and distribution of renewable energy that incorporates energy conservation and efficiency improvements.

  3. Role for DNA methylation in the regulation of miR-200c and miR-141 expression in normal and cancer cells

    SciTech Connect (OSTI)

    Vrba, Lukas; Jensen, Taylor J.; Garbe, James C.; Heimark, Ronald L.; Cress, Anne E.; Dickinson, Sally; Stampfer, Martha R.; Futscher, Bernard W.

    2009-12-23

    BACKGROUND: The microRNA-200 family participates in the maintenance of an epithelial phenotype and loss of its expression can result in epithelial to mesenchymal transition (EMT). Furthermore, the loss of expression of miR-200 family members is linked to an aggressive cancer phenotype. Regulation of the miR-200 family expression in normal and cancer cells is not fully understood. METHODOLOGY/ PRINCIPAL FINDINGS: Epigenetic mechanisms participate in the control of miR-200c and miR-141 expression in both normal and cancer cells. A CpG island near the predicted mir-200c/mir-141 transcription start site shows a striking correlation between miR-200c and miR-141 expression and DNA methylation in both normal and cancer cells, as determined by MassARRAY technology. The CpG island is unmethylated in human miR-200/miR-141 expressing epithelial cells and in miR-200c/miR-141 positive tumor cells. The CpG island is heavily methylated in human miR-200c/miR-141 negative fibroblasts and miR-200c/miR-141 negative tumor cells. Mouse cells show a similar inverse correlation between DNA methylation and miR-200c expression. Enrichment of permissive histone modifications, H3 acetylation and H3K4 trimethylation, is seen in normal miR-200c/miR-141-positive epithelial cells, as determined by chromatin immunoprecipitation coupled to real-time PCR. In contrast, repressive H3K9 dimethylation marks are present in normal miR-200c/miR-141-negative fibroblasts and miR-200c/miR-141 negative cancer cells and the permissive histone modifications are absent. The epigenetic modifier drug, 5-aza-2'-deoxycytidine, reactivates miR-200c/miR-141 expression showing that epigenetic mechanisms play a functional role in their transcriptional control. CONCLUSIONS/ SIGNIFICANCE: We report that DNA methylation plays a role in the normal cell type-specific expression of miR-200c and miR-141 and this role appears evolutionarily conserved, since similar results were obtained in mouse. Aberrant DNA methylation of the

  4. Users May Now Clear Their Own Login Failures

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Users May Now Clear Their Own Login Failures Users May Now Clear Their Own Login Failures May 16, 2013 by Francesca Verdier Users may now clear their own login failures simply by logging in to the NIM website (https://nim.nersc.gov). No further steps are necessary; that is, the simple act of logging in to NIM will clear your login failures on all NERSC compute systems. NIM will then provide a display of the number of login failures that were cleared on each compute system that was affected at

  5. Save Money with Your Very Own Drapes | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Money with Your Very Own Drapes Save Money with Your Very Own Drapes June 11, 2012 - 2:47pm Addthis Elizabeth Spencer Communicator, National Renewable Energy Laboratory So, last winter I decided that I was going to get crafty and make my own blankets as an incentive to keep the thermostat low. It was a fun project, and it actually worked. I needed more blankets, so I made them. They were warm, so I used them all the time. And hey, I was proud of them! It feels good to make something you love.

  6. miR-92a family and their target genes in tumorigenesis and metastasis

    SciTech Connect (OSTI)

    Li, Molin; Guan, Xingfang; Sun, Yuqiang; Mi, Jun; Shu, Xiaohong; Liu, Fang; Li, Chuangang

    2014-04-15

    The miR-92a family, including miR-25, miR-92a-1, miR-92a-2 and miR-363, arises from three different paralog clusters miR-17-92, miR-106a-363, and miR-106b-25 that are highly conservative in the process of evolution, and it was thought as a group of microRNAs (miRNAs) correlated with endothelial cells. Aberrant expression of miR-92a family was detected in multiple cancers, and the disturbance of miR-92a family was related with tumorigenesis and tumor development. In this review, the progress on the relationship between miR-92a family and their target genes and malignant tumors will be summarized. - Highlights: • Aberrant expression of miR-92a, miR-25 and miR-363 can be observed in many kinds of malignant tumors. • The expression of miR-92a family is regulated by LOH, epigenetic alteration, transcriptional factors such as SP1, MYC, E2F, wild-type p53 etc. • Roles of miR-92a family in tumorigenesis and development: promoting cell proliferation, invasion and metastasis, inhibiting cell apoptosis.

  7. Women-Owned Small Business Webinar, June 20, 2013

    Energy.gov [DOE]

    The Office of Small and Disadvantaged Business Utilization hosted a webinar for women-owned small businesses on June 20, 2013, to provide overviews of major program offices in the Department of...

  8. Faith Enterprises Inc. A Service Disabled Veteran-Owned Small...

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Faith Enterprises Inc. A Service Disabled Veteran-Owned Small Business Security When the Air Force asked Sandia to deliver complex security upgrades to a facility on Kirtland Air...

  9. Great opportunity for Native American-owned businesses

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Great opportunity for Native American-owned businesses Community Connections: Your link to news and opportunities from Los Alamos National Laboratory Latest Issue:November 2, 2016 all issues All Issues » submit Great opportunity for Native American-owned businesses Proposals for Native American Venture Acceleration Fund due October 18. September 1, 2016 Phoebe Suina of High Water Mark, LLC, was a Native American VAF winner in 2015. Phoebe Suina of High Water Mark, LLC, was a Native American VAF

  10. OSTI Salutes Librarians (including its own!), National Librarian Week

    Office of Scientific and Technical Information (OSTI)

    (April 8-14) | OSTI, US Dept of Energy Office of Scientific and Technical Information Salutes Librarians (including its own!), National Librarian Week (April 8-14) Back to the OSTI News Listing for 2012 Thanks to librarians across the nation for their commitment to ensuring access to information. OSTI, with its own cadre of Masters-level librarians, connects with university research departments and libraries to increase awareness of the U.S. Department of Energy's valuable scientific and

  11. Minority-Owned Business Creating Career Opportunities | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Energy Minority-Owned Business Creating Career Opportunities Minority-Owned Business Creating Career Opportunities September 15, 2010 - 2:21pm Addthis Most Catalyst Management Group employees had no previous experience with weatherization. | Photo by CMG Most Catalyst Management Group employees had no previous experience with weatherization. | Photo by CMG Lindsay Gsell What are the key facts? This Pontiac, Michigan weatherization company sees growth through Recovery Act. Catalyst Management

  12. Port Huron, MI Natural Gas Pipeline Imports From Canada (Million...

    U.S. Energy Information Administration (EIA) (indexed site)

    Million Cubic Feet) Port Huron, MI Natural Gas Pipeline Imports From Canada (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2016 262 - No Data Reported; ...

  13. miR-107 and miR-25 simultaneously target LATS2 and regulate proliferation and invasion of gastric adenocarcinoma (GAC) cells

    SciTech Connect (OSTI)

    Zhang, Mingjun; Wang, Xiaolei; Li, Wanhu; Cui, Yongchun

    2015-05-08

    Although a series of oncogenes and tumor suppressors were identified in the pathological development of gastric adenocarcinoma (GAC), the underlying molecule mechanism were still not fully understood. The current study explored the expression profile of miR-107 and miR-25 in GAC patients and their downstream regulative network. qRT-PCR analysis was performed to quantify the expression of these two miRNAs in serum samples from both patients and healthy controls. Dual luciferase assay was conducted to verify their putative bindings with LATS2. MTT assay, cell cycle assay and transwell assay were performed to explore how miR-107 and miR-25 regulate proliferation and invasion of gastric cancer cells. Findings of this study demonstrated that total miR-107 or miR-25 expression might be overexpressed in gastric cancer patients and they can simultaneously and synchronically regulate LATS2 expression, thereby affecting gastric cancer cell growth and invasion. Therefore, the miR-25/miR-107-LATS2 axis might play an important role in proliferation and invasion of the gastric cancer cells. - Highlights: • Total miR-107 and miR-25 expression is significantly increased in GAC patients. • Both miR-107 and miR-25 can promote proliferation and invasion of GAC cells. • Both miR-107 and miR-25 can target LATS2 and regulate its expression. • miR-107 and miR-25 regulate proliferation and invasion of GAC cells though LATS2.

  14. Empirical test of the effects of Internal Revenue Code Section 465 on risk-taking by investors in oil and gas drilling programs

    SciTech Connect (OSTI)

    Christian, C.W.

    1985-01-01

    Taxation affects the cash flows generated by financial investments, and, under some conditions, it also affects the degree of risk investors are willing to bear. This study investigates the effects of the Internal Revenue Code Section 465 on risk-taking by financial investors in oil and gas drilling programs. Section 465 added new rules limiting loss deductions from certain activities, explicitly including oil and gas drilling. Prior research reached varying conclusions analytically, but most research concurs that investor risk-taking is reduced when a tax structure reduces loss-offsetting, i.e., reduces the deductibility of investment losses against other income. Section 465 does that under certain circumstances, so it presents an opportunity to empirically reexamine the question. This study presents null hypotheses that state that the percentage of limited-partner investment in drilling programs with different drilling objectives and deal term structures (and different levels of risk) was unchanged between the time periods before and after the enactment of Section 465. The study concludes that the loss deduction limitations of I.R.C. Section 465 did play a role in the reduction of risk-taking by limited partners in oil and gas drilling programs.

  15. miR-17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression

    SciTech Connect (OSTI)

    Gao, Yong; Luo, Ling-hui; Li, Shuai; Yang, Cao

    2014-02-07

    Highlights: • miR-17 was increased in OS tissues and cell lines. • Inhibition of miR-17 suppressed OS cell proliferation. • Inhibition of miR-17 suppressed OS cell migration and invasion. • PTEN was a target of miR-17. • miR-17 was negatively correlated with PTEN in OS tissues. - Abstract: MicroRNAs (miRNAs) play essential roles in cancer development and progression. Here, we investigated the role of miR-17 in the progression and metastasis of osteosarcoma (OS). miR-17 was frequently increased in OS tissues and cell lines. Inhibition of miR-17 in OS cell lines substantially suppressed cell proliferation, migration, and invasion. Phosphatase and tensin homolog (PTEN) was identified as a target of miR-17, and ectopic expression of miR-17 inhibited PTEN by direct binding to its 3′-untranslated region (3′-UTR). Expression of miR-17 was negatively correlated with PTEN in OS tissues. Together, these findings indicate that miR-17 acts as an oncogenic miRNA and may contribute to the progression and metastasis of OS, suggesting miR-17 as a potential novel diagnostic and therapeutic target of OS.

  16. Safety of Department of Energy-Owned Nuclear Reactors

    Directives, Delegations, and Requirements [Office of Management (MA)]

    1986-09-23

    To establish reactor safety program requirements assure that the safety of each Department of Energy-owned (DOE-owned) reactor is properly analyzed, evaluated, documented, and approved by DOE; and reactors are sited, designed, constructed, modified, operated, maintained, and decommissioned in a manner that gives adequate protection for health and safety and will be in accordance with uniform standards, guides, and codes which are consistent with those applied to comparable licensed reactors. Cancels Chap. 6 of DOE O 5480.1A. Paragraphs 7b(3), 7e(3) & 8c canceled by DOE O 5480.23 & canceled by DOE N 251.4 of 9-29-95.

  17. MiR-218 Mediates tumorigenesis and metastasis: Perspectives and implications

    SciTech Connect (OSTI)

    Lu, Ying-fei; Zhang, Li; Waye, Mary Miu Yee; Fu, Wei-ming; Zhang, Jin-fang

    2015-05-15

    MicroRNAs (miRNAs) are a class of small non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. As a highly conserved miRNA across a variety of species, microRNA-218 (miR-218) was found to play pivotal roles in tumorigenesis and progression. A group of evidence has demonstrated that miR-218 acts as a tumor suppressor by targeting many oncogenes related to proliferation, apoptosis and invasion. In this review, we provide a complex overview of miR-218, including its regulatory mechanisms, known functions in cancer and future challenges as a potential therapeutic target in human cancers. - Highlights: • miR-218 is frequently down regulated in multiple cancers. • miR-218 plays pivotal roles in carcinogenesis. • miR-218 mediates proliferation, apoptosis, metastasis, invasion, etc. • miR-218 mediates tumorigenesis and metastasis via multiple pathways.

  18. Financial statistics of major US publicly owned electric utilities 1992

    SciTech Connect (OSTI)

    Not Available

    1994-01-01

    The 1992 edition of the Financial Statistics of Major US Publicly Owned Electric Utilities publication presents 4 years (1989 through 1992) of summary financial data and current year detailed financial data on the major publicly owned electric utilities. The objective of the publication is to provide Federal and State governments, industry, and the general public with current and historical data that can be used for policymaking and decisionmaking purposes related to publicly owned electric utility issues. Generator and nongenerator summaries are presented in this publication. Four years of summary financial data are provided. Summaries of generators for fiscal years ending June 30 and December 31, nongenerators for fiscal years ending June 30 and December 31, and summaries of all respondents are provided. The composite tables present aggregates of income statement and balance sheet data, as well as financial indicators. Composite tables also display electric operation and maintenance expenses, electric utility plant, number of consumers, sales of electricity, and operating revenue, and electric energy account data. The primary source of publicly owned financial data is the Form EIA-412, {open_quotes}Annual Report of Public Electric Utilities.{close_quotes} Public electric utilities file this survey on a fiscal year, rather than a calendar year basis, in conformance with their recordkeeping practices. In previous editions of this publication, data were aggregated by the two most commonly reported fiscal years, June 30 and December 31. This omitted approximately 20 percent of the respondents who operate on fiscal years ending in other months. Accordingly, the EIA undertook a review of the Form EIA-412 submissions to determine if alternative classifications of publicly owned electric utilities would permit the inclusion of all respondents.

  19. NuMI Low Energy Flux Prediction Release

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    NuMI Low Energy Flux Prediction Release Neutrino Flux Predictions for the NuMI Beam hep-ex/1607.00704 Data Ancillary data files for this result are available on arXiv at http://arxiv.org/src/1607.00704/anc Among the available data files are: pdf file describing format of all the available files root file of all the available fluxes python code to read and process MINERvA's flux predictions Text Files of the flux, uncertainties, and covariance matrix, with units of neutrinos/m^2/POT, in 0.5 GeV

  20. miRNA-205 affects infiltration and metastasis of breast cancer

    SciTech Connect (OSTI)

    Wang, Zhouquan; Department of Tumor, SenGong Hospital of Shaanxi, Xian 710300 ; Liao, Hehe; Deng, Zhiping; Yang, Po; Du, Ning; Zhanng, Yunfeng; Ren, Hong

    2013-11-08

    Highlights: We detected expression of miR-205 in breast cancer cell lines and tissue samples. We suggest miR-205 is downregulated in human breast cancer tissues and MCF7 cells. We suggest the lower expression of miR-205 play a role in breast cancer onset. These data suggest that miR-205 directly targets HER3 in human breast cancer. -- Abstract: Background: An increasing number of studies have shown that miRNAs are commonly deregulated in human malignancies, but little is known about the function of miRNA-205 (miR-205) in human breast cancer. The present study investigated the influence of miR-205 on breast cancer malignancy. Methods: The expression level of miR-205 in the MCF7 breast cancer cell line was determined by quantitative (q)RT-PCR. We then analyzed the expression of miR-205 in breast cancer and paired non-tumor tissues. Finally, the roles of miR-205 in regulating tumor proliferation, apoptosis, migration, and target gene expression were studied by MTT assay, flow cytometry, qRT-PCR, Western blotting and luciferase assay. Results: miR-205 was downregulated in breast cancer cells or tissues compared with normal breast cell lines or non-tumor tissues. Overexpression of miR-205 reduced the growth and colony-formation capacity of MCF7 cells by inducing apoptosis. Overexpression of miR-205 inhibited MCF7 cell migration and invasiveness. By bioinformation analysis, miR-205 was predicted to bind to the 3? untranslated regions of human epidermal growth factor receptor (HER)3 mRNA, and upregulation of miR-205 reduced HER3 protein expression. Conclusion: miR-205 is a tumor suppressor in human breast cancer by post-transcriptional inhibition of HER3 expression.

  1. Ground Motion Studies at NuMI

    SciTech Connect (OSTI)

    Mayda M. Velasco; Michal Szleper

    2012-02-20

    Ground motion can cause significant deterioration in the luminosity of a linear collider. Vibration of numerous focusing magnets causes continuous misalignments, which makes the beam emittance grow. For this reason, understanding the seismic vibration of all potential LC sites is essential and related efforts in many sites are ongoing. In this document we summarize the results from the studies specific to Fermilab grounds as requested by the LC project leader at FNAL, Shekhar Mishra in FY04-FY06. The Northwestern group focused on how the ground motion effects vary with depth. Knowledge of depth dependence of the seismic activity is needed in order to decide how deep the LC tunnel should be at sites like Fermilab. The measurements were made in the NuMI tunnel, see Figure 1. We take advantage of the fact that from the beginning to the end of the tunnel there is a height difference of about 350 ft and that there are about five different types of dolomite layers. The support received allowed to pay for three months of salary of Michal Szleper. During this period he worked a 100% of his time in this project. That include one week of preparation: 2.5 months of data taking and data analysis during the full period of the project in order to guarantee that we were recording high quality data. We extended our previous work and made more systematic measurements, which included detailed studies on stability of the vibration amplitudes at different depths over long periods of time. As a consequence, a better control and more efficient averaging out of the daytime variation effects were possible, and a better study of other time dependences before the actual depth dependence was obtained. Those initial measurements were made at the surface and are summarized in Figure 2. All measurements are made with equipment that we already had (two broadband seismometers KS200 from GEOTECH and DL-24 portable data recorder). The offline data analysis took advantage of the full Fourier spectra

  2. DOE2016_Socioeconomic_Programs_Women_Owned_Small_Business

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Women-Owned Small Business Federal Contract Program Ann Sullivan President Madison Services Group, Inc. 2 The WOSB Program - 15 Years of Advocacy * Establishment * Leveling the playing field * Program expansion * Success 3 The WOSB Program - A Brief History * WOSB Program established in 2000 * Original disparity study released in 2007 * Program implemented in 2011 4 How It Works * Contracting officers can restrict competition to WOSBs/EDWOSBs if: - Fair and reasonable price - Rule of two - In

  3. Financial statistics of major US publicly owned electric utilities 1994

    SciTech Connect (OSTI)

    1995-12-15

    This publication presents 5 years (1990--94) of summary financial data and current year detailed financial data on the major publicly owned electric utilities. Generator and nongenerator summaries are presented. Composite tables present: Aggregates of income statement and balance sheet data, financial indicators, electric operation and maintenance expenses, electric utility plant, number of consumers, sales of electricity, and operating revenue, and electric energy account data.

  4. Dianne Williams Wilburn-Creating her own destiny

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Dianne Williams Wilburn Dianne Williams Wilburn-Creating her own destiny Having monitored environmental compliance for New Mexico State and analyzed water chemistry for a nuclear power plant in Virginia, Wilburn is well versed in environmental health and radiation safety. March 11, 2014 Dianne Williams Wilburn Having monitored environmental compliance for New Mexico State and analyzed water chemistry for a nuclear power plant in Virginia, Wilburn is well versed in environmental health and

  5. Financial statistics major US publicly owned electric utilities 1996

    SciTech Connect (OSTI)

    1998-03-01

    The 1996 edition of The Financial Statistics of Major US Publicly Owned Electric Utilities publication presents 5 years (1992 through 1996) of summary financial data and current year detailed financial data on the major publicly owned electric utilities. The objective of the publication is to provide Federal and State governments, industry, and the general public with current and historical data that can be used for policymaking and decision making purposes related to publicly owned electric utility issues. Generator and nongenerator summaries are presented in this publication. Five years of summary financial data are provided. Summaries of generators for fiscal years ending June 30 and December 31, nongenerators for fiscal years ending June 30 and December 31, and summaries of all respondents are provided. The composite tables present aggregates of income statement and balance sheet data, as well as financial indicators. Composite tables also display electric operation and maintenance expenses, electric utility plant, number of consumers, sales of electricity, and operating revenue, and electric energy account data. 2 figs., 32 tabs.

  6. DOE - Office of Legacy Management -- Mitts-Merrill Co - MI 14

    Office of Legacy Management (LM)

    1993 MI.14-2 Site Operations: Reduced thorium metal chunks into particle sized pieces ... Primary Radioactive Materials Handled: Thorium MI.14-1 Radiological Survey(s): Yes - ...

  7. DOE - Office of Legacy Management -- Mitts-Merrel Co - MI 14

    Office of Legacy Management (LM)

    1993 MI.14-2 Site Operations: Reduced thorium metal chunks into particle sized pieces ... Primary Radioactive Materials Handled: Thorium MI.14-1 Radiological Survey(s): Yes - ...

  8. MiR-125a TNF receptor-associated factor 6 to inhibit osteoclastogenesis

    SciTech Connect (OSTI)

    Guo, Li-Juan; Liao, Lan; Yang, Li; Li, Yu; Jiang, Tie-Jian

    2014-02-15

    MicroRNAs (miRNAs) play important roles in osteoclastogenesis and bone resorption. In the present study, we found that miR-125a was dramatically down-regulated during macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor-κB ligand (RANKL) induced osteoclastogenesis of circulating CD14+ peripheral blood mononuclear cells (PBMCs). Overexpression of miR-125a in CD14+ PBMCs inhibited osteoclastogenesis, while inhibition of miR-125a promoted osteoclastogenesis. TNF receptor-associated factor 6 (TRAF6), a transduction factor for RANKL/RANK/NFATc1 signal, was confirmed to be a target of miR-125a. EMSA and ChIP assays confirmed that NFATc1 bound to the promoter of the miR-125a. Overexpression of NFATc1 inhibited miR-125a transcription, and block of NFATc1 expression attenuated RANKL-regulated miR-125a transcription. Here, we reported that miR-125a played a biological function in osteoclastogenesis through a novel TRAF6/ NFATc1/miR-125a regulatory feedback loop. It suggests that regulation of miR-125a expression may be a potential strategy for ameliorating metabolic disease. - Highlights: • MiR-125a was significantly down-regulated in osteoclastogenesis of CD14+ PBMCs. • MiR-125a inhibited osteoclast differentiation by targeting TRAF6. • NFATc1 inhibited miR-125a transciption by binding to the promoter of miR-125a. • TRAF6/NFATc1 and miR-125a form a regulatory feedback loop in osteoclastogenesis.

  9. Port Huron, MI Liquefied Natural Gas Exports (Million Cubic Feet...

    U.S. Energy Information Administration (EIA) (indexed site)

    (Million Cubic Feet) Port Huron, MI Liquefied Natural Gas Exports (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2013 1 2014 1 1 1 1 2 1 1 1 1 1 2015 1 1...

  10. Neutron scattering study of spin ordering and stripe pinning in superconducting <mi>La>1.93<mi>Sr>0.07<mi>CuO>4

    SciTech Connect (OSTI)

    Jacobsen, H.; Zaliznyak, I. A.; Savici, A. T.; Winn, B. L.; Chang, S.; Hücker, M.; Gu, G. D.; Tranquada, J. M.

    2015-11-20

    The relationships among charge order, spin fluctuations, and superconductivity in underdoped cuprates remain controversial. We use neutron scattering techniques to study these phenomena in <mi>La>1.93<mi>Sr>0.07<mi>CuO>4 a superconductor with a transition temperature of Tc = 20 K. At T<< Tc, we find incommensurate spin fluctuations with a quasielastic energy spectrum and no sign of a gap within the energy range from 0.2 to 15 meV. A weak elastic magnetic component grows below ~ 10 K, consistent with results from local probes. Regarding the atomic lattice, we have discovered unexpectedly strong fluctuations of the CuO6 octahedra about Cu-O bonds, which are associated with inequivalent O sites within the CuO2 planes. Moreover, we observed a weak elastic (3 30) superlattice peak that implies a reduced lattice symmetry. The presence of inequivalent O sites rationalizes various pieces of evidence for charge stripe order in underdoped La2-xSrxCuO4. The coexistence of superconductivity with quasi-static spin-stripe order suggests the presence of intertwined orders; however, the rotation of the stripe orientation away from the Cu-O bonds might be connected with evidence for a finite gap at the nodal points of the superconducting gap function.

  11. MiR-145 functions as a tumor suppressor targeting NUAK1 in human intrahepatic cholangiocarcinoma

    SciTech Connect (OSTI)

    Xiong, Xinkui; Sun, Daoyi; Chai, Hao; Shan, Wengang; Yu, Yue; Pu, Liyong; Cheng, Feng

    2015-09-18

    The dysregulation of micro (mi)RNAs is associated with cancer development. The miRNA miR-145 is downregulated in intrahepatic cholangiocarcinoma (ICC); however, its precise role in tumor progression has not yet been elucidated. Novel (nua) kinase family (NUAK)1 functions as an oncogene in various cancers and is a putative target of miR-145 regulation. In this study, we investigated the regulation of NUAK1 by miR-145 in ICC. We found that miR-145 level was significantly decreased in ICC tissue and cell lines, which corresponded with an increase in NUAK1 expression. NUAK1 was found to be a direct target of miR-145 regulation. The overexpression of miR-145 in ICC cell lines inhibited proliferation, growth, and invasion by suppressing NUAK1 expression, which was associated with a decrease in Akt signaling and matrix metalloproteinase protein expression. Similar results were observed by inhibiting NUAK1 expression. These results demonstrate that miR-145 can prevent ICC progression by targeting NUAK1 and its downstream effectors, and can therefore be useful for clinical diagnosis and targeted therapy of ICC. - Highlights: • MiR-145 suppresses ICC proliferation and invasion abilities. • We demonstrated that miR-145 directly targets NUAK1 in ICC. • MiR-145 expression in ICC was associated with Akt signaling and MMPs expression.

  12. Radiosensitizing Effects of Ectopic miR-101 on Non-Small-Cell Lung Cancer Cells Depend on the Endogenous miR-101 Level

    SciTech Connect (OSTI)

    Chen, Susie; Wang Hongyan; Ng, Wooi Loon; Curran, Walter J.; Wang Ya

    2011-12-01

    Purpose: Previously, we showed that ectopic miR-101 could sensitize human tumor cells to radiation by targeting ATM and DNA-PK catalytic subunit (DNA-PKcs) to inhibit DNA repair, as the endogenous miR-101 levels are low in tumors in general. However, the heterogeneity of human cancers may result in an exception. The purpose of this study was to test the hypothesis that a few tumor cell lines with a high level of endogenous miR-101 would prove less response to ectopic miR-101. Methods and Materials: Fourteeen non-small-cell lung cancer (NSCLC) cell lines and one immortalized non-malignant lung epithelial cell line (NL20) were used for comparing endogenous miR-101 levels by real-time reverse transcription-polymerase chain reaction. Based on the different miR-101 levels, four cell lines with different miR-101 levels were chosen for transfection with a green fluorescent protein-lentiviral plasmid encoding miR-101. The target protein levels were measured by using Western blotting. The radiosensitizing effects of ectopic miR-101 on these NSCLC cell lines were determined by a clonogenic assay and xenograft mouse model. Results: The endogenous miR-101 level was similar or lower in 13 NSCLC cell lines but was 11-fold higher in one cell line (H157) than in NL20 cells. Although ectopic miR-101 efficiently decreased the ATM and DNA-PKcs levels and increased the radiosensitization level in H1299, H1975, and A549 cells, it did not change the levels of the miR-101 targets or radiosensitivity in H157 cells. Similar results were observed in xenograft mice. Conclusions: A small number of NSCLC cell lines could have a high level of endogenous miR-101. The ectopic miR-101 was able to radiosensitize most NSCLC cells, except for the NSCLC cell lines that had a much higher endogenous miR-101 level. These results suggest that when we choose one miRNA as a therapeutic tool, the endogenous level of the miRNA in each tumor should be considered.

  13. Transportation capabilities study of DOE-owned spent nuclear fuel

    SciTech Connect (OSTI)

    Clark, G.L.; Johnson, R.A.; Smith, R.W.; Abbott, D.G.; Tyacke, M.J.

    1994-10-01

    This study evaluates current capabilities for transporting spent nuclear fuel owned by the US Department of Energy. Currently licensed irradiated fuel shipping packages that have the potential for shipping the spent nuclear fuel are identified and then matched against the various spent nuclear fuel types. Also included are the results of a limited investigation into other certified packages and new packages currently under development. This study is intended to support top-level planning for the disposition of the Department of Energy`s spent nuclear fuel inventory.

  14. PPARγ inhibits ovarian cancer cells proliferation through upregulation of miR-125b

    SciTech Connect (OSTI)

    Luo, Shuang; Wang, Jidong; Ma, Ying; Yao, Zhenwei; Pan, Hongjuan

    2015-06-26

    miR-125b has essential roles in coordinating tumor proliferation, angiogenesis, invasiveness, metastasis and chemotherapy recurrence. In ovarian cancer miR-125b has been shown to be downregulated and acts as a tumor suppressor by targeting proto-oncogene BCL3. PPARγ, a multiple functional transcription factor, has been reported to have anti-tumor effects through inhibition of proliferation and induction of differentiation and apoptosis by targeting the tumor related genes. However, it is unclear whether miR-125b is regulated by PPARγ in ovarian cancer. In this study, we demonstrated that the miR-125b downregulated in ovarian cancer tissues and cell lines. Ligands-activated PPARγ suppressed proliferation of ovarian cancer cells and this PPARγ-induced growth inhibition is mediated by the upregulation of miR-125b. PPARγ promoted the expression of miR-125b by directly binding to the responsive element in miR-125b gene promoter region. Thus, our results suggest that PPARγ can induce growth suppression of ovarian cancer by upregulating miR-125b which inhibition of proto-oncogene BCL3. These findings will extend our understanding of the function of PPARγ in tumorigenesis and miR-125b may be a therapeutic intervention of ovarian cancer. - Highlights: • miR-125b is down-regulated in ovarian cancer tissues and cells. • PPARγ upregulates miR-125b and downregulates its target gene BCL3 expression. • Silence of miR-125b attenuates PPARγ-mediated growth suppression of ovarian cancer cells. • PPARγ promotes the transcription of miR-125b via binding to PPARE in miR-125b gene promoter region.

  15. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    which requires both electric and natural gas investor-owned utilities to reduce energy sales, and spend a minimum ... Eligibility: Investor-Owned Utility, Retail Supplier...

  16. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Act 295, requiring the state's investor-owned utilities, alternative retail suppliers, electric cooperativ... Eligibility: Investor-Owned Utility, Municipal Utilities,...

  17. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    that created the Illinois Power Agency (IPA). The agency's purpose is to develop electricity procurement plans for investor-owne... Eligibility: Investor-Owned Utility,...

  18. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Virginia Wisconsin Wyoming Apply Current search Tankless Water Heater Remove Tankless Water Heater filter Investor-Owned Utility Remove Investor-Owned Utility filter Filter by...

  19. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Public Act 295, requiring the state's investor-owned utilities, alternative retail suppliers, electric cooperativ... Eligibility: Investor-Owned Utility, Municipal Utilities,...

  20. V-033: ownCloud Cross-Site Scripting and File Upload Vulnerabilities |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy 3: ownCloud Cross-Site Scripting and File Upload Vulnerabilities V-033: ownCloud Cross-Site Scripting and File Upload Vulnerabilities November 26, 2012 - 2:00am Addthis PROBLEM: ownCloud Cross-Site Scripting and File Upload Vulnerabilities PLATFORM: ownCloud 4.5.2, 4.5.1, 4.0.9 ABSTRACT: Multiple vulnerabilities have been reported in ownCloud REFERENCE LINKS: ownCloud Server Advisories Secunia Advisory SA51357 IMPACT ASSESSMENT: Medium DISCUSSION: 1) Input passed via the

  1. A Look at Health Care Buildings - Who owns and occupies them

    U.S. Energy Information Administration (EIA) (indexed site)

    Who Owns? Return to: A Look at Health Care Buildings How large are they? How many employees are there? Where are they located? How old are they? Who owns and occupies them? Are...

  2. POLICY FLASH 2013-59 Class Deviation (FAR) 19.15, Women-Owned...

    Energy Savers

    POLICY FLASH 2013-59 Class Deviation (FAR) 19.15, Women-Owned Small Business (WOSB) Program POLICY FLASH 2013-59 Class Deviation (FAR) 19.15, Women-Owned Small Business (WOSB)...

  3. Financial statistics of selected publicly owned electric utilities 1989. [Contains glossary

    SciTech Connect (OSTI)

    Not Available

    1991-02-06

    The Financial Statistics of Selected Publicly Owned Electric Utilities publication presents summary and detailed financial accounting data on the publicly owned electric utilities. The objective of the publication is to provide the Federal and State governments, industry, and the general public with data that can be used for policymaking and decision making purposes relating to publicly owned electric utility issues. 21 tabs.

  4. Genome-Wide Analysis of miRNA targets in Brachypodium and Biomass Energy Crops

    SciTech Connect (OSTI)

    Green, Pamela J.

    2015-08-11

    MicroRNAs (miRNAs) contribute to the control of numerous biological processes through the regulation of specific target mRNAs. Although the identities of these targets are essential to elucidate miRNA function, the targets are much more difficult to identify than the small RNAs themselves. Before this work, we pioneered the genome-wide identification of the targets of Arabidopsis miRNAs using an approach called PARE (German et al., Nature Biotech. 2008; Nature Protocols, 2009). Under this project, we applied PARE to Brachypodium distachyon (Brachypodium), a model plant in the Poaceae family, which includes the major food grain and bioenergy crops. Through in-depth global analysis and examination of specific examples, this research greatly expanded our knowledge of miRNAs and target RNAs of Brachypodium. New regulation in response to environmental stress or tissue type was found, and many new miRNAs were discovered. More than 260 targets of new and known miRNAs with PARE sequences at the precise sites of miRNA-guided cleavage were identified and characterized. Combining PARE data with the small RNA data also identified the miRNAs responsible for initiating approximately 500 phased loci, including one of the novel miRNAs. PARE analysis also revealed that differentially expressed miRNAs in the same family guide specific target RNA cleavage in a correspondingly tissue-preferential manner. The project included generation of small RNA and PARE resources for bioenergy crops, to facilitate ongoing discovery of conserved miRNA-target RNA regulation. By associating specific miRNA-target RNA pairs with known physiological functions, the research provides insights about gene regulation in different tissues and in response to environmental stress. This, and release of new PARE and small RNA data sets should contribute basic knowledge to enhance breeding and may suggest new strategies for improvement of biomass energy crops.

  5. DLEU2, frequently deleted in malignancy, functions as a critical host gene of the cell cycle inhibitory microRNAs miR-15a and miR-16-1

    SciTech Connect (OSTI)

    Lerner, Mikael; Harada, Masako; Loven, Jakob; Castro, Juan; Davis, Zadie; Oscier, David; Henriksson, Marie; Sangfelt, Olle; Grander, Dan; Corcoran, Martin M.

    2009-10-15

    The microRNAs miR-15a and miR-16-1 are downregulated in multiple tumor types and are frequently deleted in chronic lymphocytic leukemia (CLL), myeloma and mantle cell lymphoma. Despite their abundance in most cells the transcriptional regulation of miR-15a/16-1 remains unclear. Here we demonstrate that the putative tumor suppressor DLEU2 acts as a host gene of these microRNAs. Mature miR-15a/miR-16-1 are produced in a Drosha-dependent process from DLEU2 and binding of the Myc oncoprotein to two alterative DLEU2 promoters represses both the host gene transcript and levels of mature miR-15a/miR-16-1. In line with a functional role for DLEU2 in the expression of the microRNAs, the miR-15a/miR-16-1 locus is retained in four CLL cases that delete both promoters of this gene and expression analysis indicates that this leads to functional loss of mature miR-15a/16-1. We additionally show that DLEU2 negatively regulates the G1 Cyclins E1 and D1 through miR-15a/miR-16-1 and provide evidence that these oncoproteins are subject to miR-15a/miR-16-1-mediated repression under normal conditions. We also demonstrate that DLEU2 overexpression blocks cellular proliferation and inhibits the colony-forming ability of tumor cell lines in a miR-15a/miR-16-1-dependent way. Together the data illuminate how inactivation of DLEU2 promotes cell proliferation and tumor progression through functional loss of miR-15a/miR-16-1.

  6. Microfluidic Molecular Assay Platform for the Detection of miRNAs...

    Office of Scientific and Technical Information (OSTI)

    Article: Microfluidic Molecular Assay Platform for the Detection of miRNAs, mRNAs, Proteins, and Post-translational Modifications at Single-cell Resolution. Citation Details...

  7. DOE - Office of Legacy Management -- Baker-Perkins Co - MI 13

    Office of Legacy Management (LM)

    Site Disposition: Eliminated - Potential for contamination remote based on limited scope of activities at the site MI.13-3 Radioactive Materials Handled: Yes Primary ...

  8. miR-4295 promotes cell proliferation and invasion in anaplastic thyroid carcinoma via CDKN1A

    SciTech Connect (OSTI)

    Shao, Mingchen; Geng, Yiwei; Lu, Peng; Xi, Ying; Wei, Sidong; Wang, Liuxing; Fan, Qingxia; Ma, Wang

    2015-09-04

    MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. However, the role of microRNAs in anaplastic thyroid carcinoma (ATC), has remained elusive. Here, we identified that miR-4295 promotes ATC cell proliferation by negatively regulates its target gene CDKN1A. In ATC cell lines, CCK-8 proliferation assay indicated that the cell proliferation was promoted by miR-4295, while miR-4295 inhibitor significantly inhibited the cell proliferation. Transwell assay showed that miR-4295 mimics significantly promoted the migration and invasion of ATC cells, whereas miR-4295 inhibitors significantly reduced cell migration and invasion. luciferase assays confirmed that miR-4295 directly bound to the 3'untranslated region of CDKN1A, and western blotting showed that miR-4295 suppressed the expression of CDKN1A at the protein levels. This study indicated that miR-4295 negatively regulates CDKN1A and promotes proliferation and invasion of ATC cell lines. Thus, miR-4295 may represent a potential therapeutic target for ATC intervention. - Highlights: • miR-4295 mimics promote the proliferation and invasion of ATC cells. • miR-4295 inhibitors inhibit the proliferation and invasion of ATC cells. • miR-4295 targets 3′UTR of CDKN1A in ATC cells. • miR-4295 negatively regulates CDKN1A in ATC cells.

  9. Groundwater protection for the NuMI project

    SciTech Connect (OSTI)

    Wehmann, A.; Smart, W.; Menary, S.; Hylen, J.; Childress, S.

    1997-10-01

    The physics requirements for the long base line neutrino oscillation experiment MINOS dictate that the NuMI beamline be located in the aquifer at Fermilab. A methodology is described for calculating the level of radioactivation of groundwater caused by operation of this beamline. A conceptual shielding design for the 750 meter long decay pipe is investigated which would reduce radioactivation of the groundwater to below government standards. More economical shielding designs to meet these requirements are being explored. Also, information on local geology, hydrogeology, government standards, and a glossary have been included.

  10. MiR-153 inhibits migration and invasion of human non-small-cell lung cancer by targeting ADAM19

    SciTech Connect (OSTI)

    Shan, Nianxi; Shen, Liangfang; Wang, Jun; He, Dan; Duan, Chaojun

    2015-01-02

    Highlights: • Decreased miR-153 and up-regulated ADAM19 are correlated with NSCLC pathology. • MiR-153 inhibits the proliferation and migration and invasion of NSCLC cells in vitro. • ADAM19 is a direct target of miR-153. • ADAM19 is involved in miR-153-suppressed migration and invasion of NSCLC cells. - Abstract: MiR-153 was reported to be dysregulated in some human cancers. However, the function and mechanism of miR-153 in lung cancer cells remains unknown. In this study, we investigated the role of miR-153 in human non-small-cell lung cancer (NSCLC). Using qRT-PCR, we demonstrated that miR-153 was significantly decreased in clinical NSCLC tissues and cell lines, and downregulation of miR-153 was significantly correlated with lymph node status. We further found that ectopic expression of miR-153 significantly inhibited the proliferation and migration and invasion of NSCLC cells in vitro, suggesting that miR-153 may be a novel tumor suppressor in NSCLC. Further integrated analysis revealed that ADAM19 is as a direct and functional target of miR-153. Luciferase reporter assay demonstrated that miR-153 directly targeted 3′UTR of ADAM19, and correlation analysis revealed an inverse correlation between miR-153 and ADAM19 mRNA levels in clinical NSCLC tissues. Knockdown of ADAM19 inhibited migration and invasion of NSCLC cells which was similar with effects of overexpression of miR-153, while overexpression of ADAM19 attenuated the function of miR-153 in NSCLC cells. Taken together, our results highlight the significance of miR-153 and ADAM19 in the development and progression of NSCLC.

  11. miR-129 suppresses tumor cell growth and invasion by targeting PAK5 in hepatocellular carcinoma

    SciTech Connect (OSTI)

    Zhai, Jian; Qu, Shuping; Li, Xiaowei; Zhong, Jiaming; Chen, Xiaoxia; Qu, Zengqiang; Wu, Dong

    2015-08-14

    Emerging evidence suggests that microRNAs (miRNAs) play important roles in regulating HCC development and progression; however, the mechanisms by which their specific functions and mechanisms remained to be further explored. miR-129 has been reported in gastric cancers, lung cancer and colon cancer. In this study, we disclosed a new tumor suppresser function of miR-129 in HCC. We also found the downregulation of miR-129 occurred in nearly 3/4 of the tumors examined (56/76) compared with adjacent nontumorous tissues, which was more importantly, correlated to the advanced stage and vascular invasion. We then demonstrated that miR-129 overexpression attenuated HCC cells proliferation and invasion, inducing apoptosis in vitro. Moreover, we used miR-129 antagonist and found that anti-miR-129 promoted HCC cells malignant phenotypes. Mechanistically, our further investigations revealed that miR-129 suppressed cell proliferation and invasion by targeting the 3’-untranslated region of PAK5, as well as miR-129 silencing up-regulated PAK5 expression. Moreover, miR-129 expression was inversely correlated with PAK5 expression in 76 cases of HCC samples. RNA interference of PAK5 attenuated anti-miR-129 mediated cell proliferation and invasion in HCC cells. Taken together, these results demonstrated that miR-129 suppressed tumorigenesis and progression by directly targeting PAK5, defining miR-129 as a potential treatment target for HCC. - Highlights: • Decreased of miR-129 is found in HCC and associated with advanced stage and metastasis. • miR-129 suppresses proliferation and invasion of HCC cells. • miR-129 directly targets the 3′ UTR of PAK5 and diminishes PAK5 expression. • PAK5 is involved in miR-129 mediated suppression functions.

  12. Transcriptional regulation of miR-146b by C/EBPβ LAP2 in esophageal cancer cells

    SciTech Connect (OSTI)

    Li, Junxia; Shan, Fabo; Xiong, Gang; Wang, Ju-Ming; Wang, Wen-Lin; Xu, Xueqing; Bai, Yun

    2014-03-28

    Highlights: • MiR-146b promotes esophageal cancer cell proliferation. • MiR-146b inhibits esophageal cancer cell apoptosis. • C/EBPβ directly binds to miR-146b promoter conserved region. • MiR-146b is up-regulated by C/EBPβ LAP2 transcriptional activation. - Abstract: Recent clinical study indicated that up-regulation of miR-146b was associated with poor overall survival of patients in esophageal squamous cell carcinoma. However, the underlying mechanism of miR-146b dysregulation remains to be explored. Here we report that miR-146b promotes cell proliferation and inhibits cell apoptosis in esophageal cancer cell lines. Mechanismly, two C/EBPβ binding motifs are located in the miR-146b promoter conserved region. Among the three isoforms of C/EBPβ, C/EBPβ LAP2 positively regulated miR-146b expression and increases miR-146b levels in a dose-dependent manner through transcription activation of miR-146b gene. Together, these results suggest a miR-146b regulatory mechanism involving C/EBPβ, which may contribute to the up-regulation of miR-146b in esophageal squamous cell carcinoma.

  13. miR-1297 mediates PTEN expression and contributes to cell progression in LSCC

    SciTech Connect (OSTI)

    Li, Xin; Wang, Hong-liang; Peng, Xin; Zhou, Hui-fang; Wang, Xin

    2012-10-19

    Highlights: Black-Right-Pointing-Pointer miR-1297 was found to be overexpressed in LSCC and contribute to the cell progression. Black-Right-Pointing-Pointer PTEN was confirmed to be a target gene of miR-1297. Black-Right-Pointing-Pointer Downregulation of PTEN can rescue the proliferation and invasion ability of miR-1297 downregulated Hep-2 cells. Black-Right-Pointing-Pointer Downregulation of miR-1297 inhibits tumor growth in vivo. -- Abstract: MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression after transcription, and are involved in cancer development. Laryngeal squamous cell carcinoma (LSCC) is one of the most common malignant neoplasms with increasing incidence in recent years. In this paper, we report the overexpression of miR-1297 in LSCC and Hep-2 cells. In addition, PTEN was identified to be directly regulated by miR-1297 through western blot and luciferase activity assay. Furthermore, downregulation of miR-1297 in Hep-2 cells was shown to inhibit cancer cell proliferation, migration, and tumor genesis. Our results document a new epigenetic mechanism for PTEN regulation in LSCC, which is crucial for the development of these tumors.

  14. miR-30a suppresses breast cancer cell proliferation and migration by targeting Eya2

    SciTech Connect (OSTI)

    Fu, Jing; Xu, Xiaojie; Kang, Lei; Zhou, Liying; Wang, Shibin; Lu, Juming; Cheng, Long; Fan, Zhongyi; Yuan, Bin; Tian, Peirong; Zheng, Xiaofei; Yu, Chengze; Ye, Qinong; Lv, Zhaohui

    2014-03-07

    Highlights: • miR-30a represses Eya2 expression by binding to the 3′-untranslated region of Eya2. • The miR-30a/EYA2 axis regulates breast cancer cell proliferation and migration. • The miR-30a/EYA2 axis modulates G1/S cell cycle progression. • The miR-30a/EYA2 axis is dysregulated in breast cancer patients. - Abstract: Eye absent (Eya) proteins are involved in cell fate determination in a broad spectrum of cells and tissues. Aberrant expression of Eya2 has been documented in a variety of cancers and correlates with clinical outcome. However, whether microRNAs (miRNAs) can regulate Eya2 expression remains unknown. Here, we show that miR-30a represses Eya2 expression by binding to the 3′-untranslated region of Eya2. Overexpression of Eya2 in miR-30a-transfected breast cancer cells effectively rescued the inhibition of cell proliferation and migration caused by miR-30a. Knockdown of Eya2 by small-interfering RNA (siRNA) in breast cancer cells mimicked the effect induced by miR-30a and abolished the ability of miR-30a to regulate breast cancer cell proliferation and migration. The miR-30a/Eya2 axis could regulate G1/S cell cycle progression, accompanied by the modulation of expression of cell cycle-related proteins, including cyclin A, cyclin D1, cyclin E, and c-Myc. Moreover, miR-30a expression was downregulated in breast cancer patients, and negatively correlated with Eya2, which was upregulated in breast cancer patients. These data suggest that the miR-30a/Eya2 axis may play an important role in breast cancer development and progression and that miR-30a activation or Eya2 inhibition may be a useful strategy for cancer treatment.

  15. Alternative Fuels Data Center: Buying and Selling Pre-Owned Alternative

    Alternative Fuels and Advanced Vehicles Data Center

    Fuel and Advanced Vehicles Vehicles Printable Version Share this resource Send a link to Alternative Fuels Data Center: Buying and Selling Pre-Owned Alternative Fuel and Advanced Vehicles to someone by E-mail Share Alternative Fuels Data Center: Buying and Selling Pre-Owned Alternative Fuel and Advanced Vehicles on Facebook Tweet about Alternative Fuels Data Center: Buying and Selling Pre-Owned Alternative Fuel and Advanced Vehicles on Twitter Bookmark Alternative Fuels Data Center: Buying

  16. Largest Federally Owned Wind Farm Breaks Ground at U.S. Weapons Facility |

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Y-12 National Security Complex Largest Federally Owned ... Largest Federally Owned Wind Farm Breaks Ground at U.S. Weapons Facility Posted: August 13, 2013 - 12:01pm WASHINGTON - Building on President Obama's Climate Action Plan, which calls for steady, responsible steps to reduce carbon pollution, the Energy Department today broke ground on the nation's largest federally owned wind project at the Pantex Plant in Amarillo, Texas. Once completed, this five-turbine 11.5 megawatt project will

  17. Office of River Protection Women-Owned Small Business Contractor Receives

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    One-Year Extension | Department of Energy Office of River Protection Women-Owned Small Business Contractor Receives One-Year Extension Office of River Protection Women-Owned Small Business Contractor Receives One-Year Extension December 19, 2012 - 10:33am Addthis Richland - - The Energy Department's Office of River Protection announced the contract for Advanced Technologies and Laboratories International Inc. (ATL) has been extended for a second one-year option. ATL, a women-owned small

  18. miR-21 modulates resistance of HR-HPV positive cervical cancer cells to radiation through targeting LATS1

    SciTech Connect (OSTI)

    Liu, Shikai; Song, Lili Zhang, Liang; Zeng, Saitian; Gao, Fangyuan

    2015-04-17

    Although multiple miRNAs are found involved in radioresistance development in HR-HPV positive (+) cervical cancer, only limited studies explored the regulative mechanism of the miRNAs. miR-21 is one of the miRNAs significantly upregulated in HR-HPV (+) cervical cancer is also significantly associated with radioresistance. However, the detailed regulative network of miR-21 in radioresistance is still not clear. In this study, we confirmed that miR-21 overexpression was associated with higher level of radioresistance in HR-HPV (+) cervical cancer patients and thus decided to further explore its role. Findings of this study found miR-21 can negatively affect radiosensitivity of HR-HPV (+) cervical cancer cells and decrease radiation induced G2/M block and increase S phase accumulation. By using dual luciferase assay, we verified a binding site between miR-21 and 3′-UTR of large tumor suppressor kinase 1 (LATS1). Through direct binding, miR-21 can regulate LATS1 expression in cervical cancer cells. LATS1 overexpression can reverse miR-21 induced higher colony formation rate and also reduced miR-21 induced S phase accumulation and G2/M phase block reduction under radiation treatment. These results suggested that miR-21-LATS1 axis plays an important role in regulating radiosensitivity. - Highlights: • miR-21 is highly expressed in HR-HPV (+) radioresistant cervical cancer patients. • miR-21 can negatively affect radiosensitivity of HR-HPV (+) cervical cancer cells. • miR-21 can decrease radiation induced G2/M block and increase S phase accumulation. • miR-21 modulates radiosensitivity cervical cancer cell by directly targeting LATS1.

  19. miR-92a is upregulated in cervical cancer and promotes cell proliferation and invasion by targeting FBXW7

    SciTech Connect (OSTI)

    Zhou, Chuanyi; Shen, Liangfang; Mao, Lei; Wang, Bing; Li, Yang; Yu, Huizhi

    2015-02-27

    MicroRNAs (miRNAs) are involved in the cervical carcinogenesis and progression. In this study, we investigated the role of miR-92a in progression and invasion of cervical cancer. MiR-92a was significantly upregulated in cervical cancer tissues and cell lines. Overexpression of miR-92a led to remarkably enhanced proliferation by promoting cell cycle transition from G1 to S phase and significantly enhanced invasion of cervical cancer cells, while its knockdown significantly reversed these cellular events. Bioinformatics analysis suggested F-box and WD repeat domain-containing 7 (FBXW7) as a novel target of miR-92a, and miR-92a suppressed the expression level of FBXW7 mRNA by direct binding to its 3′-untranslated region (3′UTR). Expression of miR-92a was negatively correlated with FBXW7 in cervical cancer tissues. Furthermore, Silencing of FBXW7 counteracted the effects of miR-92a suppression, while its overexpression reversed oncogenic effects of miR-92a. Together, these findings indicate that miR-92a acts as an onco-miRNA and may contribute to the progression and invasion of cervical cancer, suggesting miR-92a as a potential novel diagnostic and therapeutic target of cervical cancer. - Highlights: • miR-92a is elevated in cervical cancer tissues and cell lines. • miR-92a promotes cervical cancer cell proliferation, cell cycle transition from G1 to S phase and invasion. • FBXW7 is a direct target of miR-92a. • FBXW7 counteracts the oncogenic effects of miR-92a on cervical cancer cells.

  20. Financial Statistics of Major U.S. Publicly Owned Electric Utilities

    Reports and Publications

    2001-01-01

    2000 - Final issue. Presents summary financial data for 1994 through 2000 and detailed financial data for 2000 on major publicly owned electric utilities.

  1. Marysville, MI Natural Gas Pipeline Imports From Canada (Million Cubic

    U.S. Energy Information Administration (EIA) (indexed site)

    Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1990's 10 1,827 135 2000's NA NA 74 0 303 0 24 876 2,252 5,651 2010's 5,694 9,946 8,099 2,337 4,650 1,961 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 10/31/2016 Next Release Date: 11/30/2016 Referring Pages: U.S. Natural Gas Pipeline Imports by Point of Entry Marysville, MI Natural Gas Imports by Pipeli

  2. Marysville, MI Natural Gas Pipeline Imports From Canada (Dollars per

    U.S. Energy Information Administration (EIA) (indexed site)

    Thousand Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1990's 3.48 2.17 2.06 2000's NA NA 3.95 -- 7.80 -- 7.07 7.59 8.59 3.80 2010's 4.44 4.42 2.99 4.15 6.86 2.73 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 10/31/2016 Next Release Date: 11/30/2016 Referring Pages: U.S. Price of Natural Gas Pipeline Imports by Point of Entry Marysville, MI Natural Gas

  3. NuMI proton kicker extraction magnet termination resistor system

    SciTech Connect (OSTI)

    Reeves, S.R.; Jensen, C.C.; /Fermilab

    2005-05-01

    The temperature stability of the kicker magnet termination resistor assembly directly affects the field flatness and amplitude stability. Comprehensive thermal enhancements were made to the existing Main Injector resistor assembly design to satisfy NuMI performance specifications. Additionally, a fluid-processing system utilizing Fluorinert{reg_sign} FC-77 high-voltage dielectric was built to precisely control the setpoint temperature of the resistor assembly from 70 to 120F, required to maintain constant resistance during changing operational modes. The Fluorinert{reg_sign} must be continually processed to remove hazardous breakdown products caused by radiation exposure to prevent chemical attack of system components. Design details of the termination resistor assembly and Fluorinert{reg_sign} processing system are described. Early performance results will be presented.

  4. Non-canonical microRNAs miR-320 and miR-702 promote proliferation in Dgcr8-deficient embryonic stem cells

    SciTech Connect (OSTI)

    Kim, Byeong-Moo; Choi, Michael Y.

    2012-09-21

    Highlights: Black-Right-Pointing-Pointer Embryonic stem cells (ESCs) lacking non-canonical miRNAs proliferate slower. Black-Right-Pointing-Pointer miR-320 and miR-702 are two non-canonical miRNAs expressed in ESCs. Black-Right-Pointing-Pointer miR-320 and miR-702 promote proliferation of Dgcr8-deficient ESCs. Black-Right-Pointing-Pointer miR-320 targets p57 and helps to release Dgcr8-deficient ESCs from G1 arrest. Black-Right-Pointing-Pointer miR-702 targets p21 and helps to release Dgcr8-deficient ESCs from G1 arrest. -- Abstract: MicroRNAs are known to contribute significantly to stem cell phenotype by post-transcriptionally regulating gene expression. Most of our knowledge of microRNAs comes from the study of canonical microRNAs that require two sequential cleavages by the Drosha/Dgcr8 heterodimer and Dicer to generate mature products. In contrast, non-canonical microRNAs bypass the cleavage by the Drosha/Dgcr8 heterodimer within the nucleus but still require cytoplasmic cleavage by Dicer. The function of non-canonical microRNAs in embryonic stem cells (ESCs) remains obscure. It has been hypothesized that non-canonical microRNAs have important roles in ESCs based upon the phenotypes of ESC lines that lack these specific classes of microRNAs; Dicer-deficient ESCs lacking both canonical and non-canonical microRNAs have much more severe proliferation defect than Dgcr8-deficient ESCs lacking only canonical microRNAs. Using these cell lines, we identified two non-canonical microRNAs, miR-320 and miR-702, that promote proliferation of Dgcr8-deficient ESCs by releasing them from G1 arrest. This is accomplished by targeting the 3 Prime -untranslated regions of the cell cycle inhibitors p57 and p21 and thereby inhibiting their expression. This is the first report of the crucial role of non-canonical microRNAs in ESCs.

  5. miR-182 targets CHL1 and controls tumor growth and invasion in papillary thyroid carcinoma

    SciTech Connect (OSTI)

    Zhu, Hongling; Fang, Jin; Zhang, Jichen; Zhao, Zefei; Liu, Lianyong; Wang, Jingnan; Xi, Qian; Gu, Mingjun

    2014-07-18

    Highlights: miR-182 and CHL1 expression patterns are negatively correlated. CHL1 is a direct target of miR-182 in PTC cells. miR-182 suppression inhibits PTC cell growth and invasion. CHL1 is involved in miR-182-mediated cell behavior. - Abstract: In this study, we investigated the role and underlying mechanism of action of miR-182 in papillary thyroid carcinoma (PTC). Bioinformatics analysis revealed close homolog of LI (CHL1) as a potential target of miR-182. Upregulation of miR-182 was significantly correlated with CHL1 downregulation in human PTC tissues and cell lines. miR-182 suppressed the expression of CHL1 mRNA through direct targeting of the 3?-untranslated region (3?-UTR). Downregulation of miR-182 suppressed growth and invasion of PTC cells. Silencing of CHL1 counteracted the effects of miR-182 suppression, while its overexpression mimicked these effects. Our data collectively indicate that miR-182 in PTC promotes cell proliferation and invasion through direct suppression of CHL1, supporting the potential utility of miR-182 inhibition as a novel therapeutic strategy against PTC.

  6. Downregulation of miRNA-30c and miR-203a is associated with hepatitis C virus core protein-induced epithelial–mesenchymal transition in normal hepatocytes and hepatocellular carcinoma cells

    SciTech Connect (OSTI)

    Liu, Dongjing; Wu, Jilin; Liu, Meizhou; Yin, Hui; He, Jiantai; Zhang, Bo

    2015-09-04

    Hepatitis C virus (HCV) Core protein has been demonstrated to induce epithelial–mesenchymal transition (EMT) and is associated with cancer progression of hepatocellular carcinoma (HCC). However, how the Core protein regulates EMT is still unclear. In this study, HCV Core protein was overexpressed by an adenovirus. The protein levels of EMT markers were measured by Western blot. The xenograft animal model was established by inoculation of HepG2 cells. Results showed that ectopic expression of HCV core protein induced EMT in L02 hepatocytes and HepG2 tumor cells by upregulating vimentin, Sanl1, and Snal2 expression and downregulating E-cadherin expression. Moreover, Core protein downregulated miR-30c and miR-203a levels in L02 and HepG2 cells, but artificial expression of miR-30c and miR-203a reversed Core protein-induced EMT. Further analysis showed that ectopic expression of HCV core protein stimulated cell proliferation, inhibited apoptosis, and increased cell migration, whereas artificial expression of miR-30c and miR-203a significantly reversed the role of Core protein in these cell functions in L02 and HepG2 cells. In the HepG2 xenograft tumor models, artificial expression of miR-30c and miR-203a inhibited EMT and tumor growth. Moreover, L02 cells overexpressing Core protein can form tumors in nude mice. In HCC patients, HCV infection significantly shortened patients' survival time, and loss of miR-30c and miR-203 expression correlated with poor survival. In conclusion, HCV core protein downregulates miR-30c and miR-203a expression, which results in activation of EMT in normal hepatocytes and HCC tumor cells. The Core protein-activated-EMT is involved in the carcinogenesis and progression of HCC. Loss of miR-30c and miR-203a expression is a marker for the poor prognosis of HCC. - Highlights: • HCV core protein downregulates miR-30c and miR-203a expression. • Downregulation of miR-30c and miR-203a activates EMT. • Activated-EMT is involved in the

  7. miR-128 and its target genes in tumorigenesis and metastasis

    SciTech Connect (OSTI)

    Li, Molin, E-mail: molin_li@hotmail.com [Dalian Medical University, Dalian 116044 (China); Fu, Weiming [Center for Food Safety and Environmental Technology, Guangzhou Institute of Advanced Technology, Chinese Academy of Sciences, Guangzhou 511458 (China); Wo, Lulu; Shu, Xiaohong [Dalian Medical University, Dalian 116044 (China); Liu, Fang [The second affiliated hospital of Dalian Medical University, Dalian 116023 (China); Li, Chuangang, E-mail: li_chuangang@sina.com [The second affiliated hospital of Dalian Medical University, Dalian 116023 (China)

    2013-12-10

    MicroRNAs (miRNAs) are a class of endogenous, non-coding, 1824 nucleotide length single-strand RNAs that could modulate gene expression at post-transcriptional level. Previous studies have shown that miR-128 enriched in the brain plays an important role in the development of nervous system and the maintenance of normal physical functions. Aberrant expression of miR-128 has been detected in many types of human tumors and its validated target genes are involved in cancer-related biological processes such as cell proliferation, differentiation and apoptosis. In this review, we will summarize the roles of miR-128 and its target genes in tumorigenesis and metastasis. - Highlights: Aberrant expression of miR-128 can be observed in many kinds of malignant tumors. The molecular mechanisms regulating miR-128 expression are elucidated. Roles of miR-128 and its target genes in tumorigenesis and metastasis are summarized.

  8. miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation

    SciTech Connect (OSTI)

    Mizuno, Yosuke; Yagi, Ken; Tokuzawa, Yoshimi; Kanesaki-Yatsuka, Yukiko; Suda, Tatsuo; Katagiri, Takenobu; Fukuda, Toru; Maruyama, Masayoshi; Okuda, Akihiko; Amemiya, Tomoyuki; Kondoh, Yasumitsu; Tashiro, Hideo; Okazaki, Yasushi

    2008-04-04

    Although various microRNAs regulate cell differentiation and proliferation, no miRNA has been reported so far to play an important role in the regulation of osteoblast differentiation. Here we describe the role of miR-125b in osteoblastic differentiation in mouse mesenchymal stem cells, ST2, by regulating cell proliferation. The expression of miR-125b was time-dependently increased in ST2 cells, and the increase in miR-125b expression was attenuated in osteoblastic-differentiated ST2 cells induced by BMP-4. The transfection of exogenous miR-125b inhibited proliferation of ST2 cells and caused inhibition of osteoblastic differentiation. In contrast, when the endogenous miR-125b was blocked by transfection of its antisense RNA molecule, alkaline phosphatase activity after BMP-4 treatment was elevated. These results strongly suggest that miR-125b is involved in osteoblastic differentiation through the regulation of cell proliferation.

  9. miR-15a/16 Enhances Radiation Sensitivity of Non-Small Cell Lung Cancer

    Office of Scientific and Technical Information (OSTI)

    Cells by Targeting the TLR1/NF-κB Signaling Pathway (Journal Article) | SciTech Connect miR-15a/16 Enhances Radiation Sensitivity of Non-Small Cell Lung Cancer Cells by Targeting the TLR1/NF-κB Signaling Pathway Citation Details In-Document Search Title: miR-15a/16 Enhances Radiation Sensitivity of Non-Small Cell Lung Cancer Cells by Targeting the TLR1/NF-κB Signaling Pathway Purpose: Many miRNAs have been identified as essential issues and core determining factors in tumor radiation.

  10. Overexpression of miR-206 suppresses glycolysis, proliferation and migration in breast cancer cells via PFKFB3 targeting

    SciTech Connect (OSTI)

    Ge, Xin; Lyu, Pengwei; Cao, Zhang; Li, Jingruo; Guo, Guangcheng; Xia, Wanjun; Gu, Yuanting

    2015-08-07

    miRNAs, sorting as non-coding RNAs, are differentially expressed in breast tumor and act as tumor promoters or suppressors. miR-206 could suppress the progression of breast cancer, the mechanism of which remains unclear. The study here was aimed to investigate the effect of miR-206 on human breast cancers. We found that miR-206 was down-regulated while one of its predicted targets, 6-Phosphofructo-2-kinase (PFKFB3) was up-regulated in human breast carcinomas. 17β-estradiol dose-dependently decreased miR-206 expression as well as enhanced PFKFB3 mRNA and protein expression in estrogen receptor α (ERα) positive breast cancer cells. Furthermore, we identified that miR-206 directly interacted with 3′-untranslated region (UTR) of PFKFB3 mRNA. miR-206 modulated PFKFB3 expression in MCF-7, T47D and SUM159 cells, which was influenced by 17β-estradiol depending on ERα expression. In addition, miR-206 overexpression impeded fructose-2,6-bisphosphate (F2,6BP) production, diminished lactate generation and reduced cell proliferation and migration in breast cancer cells. In conclusion, our study demonstrated that miR-206 regulated PFKFB3 expression in breast cancer cells, thereby stunting glycolysis, cell proliferation and migration. - Highlights: • miR-206 was down-regulated and PFKFB3 was up-regulated in human breast carcinomas. • 17β-estradiol regulated miR-206 and PFKFB3 expression in ERα+ cancer cells. • miR-206directly interacted with 3′-UTR of PFKFB3 mRNA. • miR-206 fructose-2,6-bisphosphate (F2,6BP) impeded production and lactate generation. • miR-206 reduced cell proliferation and migration in breast cancer cells.

  11. AMENDMENT OF SOLICITATION/MODIFICATlON OF CONTRACT MI54 I See...

    National Nuclear Security Administration (NNSA)

    MI54 I See Block 16C I REQ. NO. Babcock & Wilcox Technical Services Pantex, LLC PO Box 30020 Amarillo, TX 79120 2. AMENDMENTIMODIFICATION NO. 1 3. EFFECTIVE DATE 1 4. ...

  12. File:USDA-CE-Production-GIFmaps-MI.pdf | Open Energy Information

    Open Energy Information (Open El) [EERE & EIA]

    MI.pdf Jump to: navigation, search File File history File usage Michigan Ethanol Plant Locations Size of this preview: 463 599 pixels. Other resolution: 464 600 pixels. Full...

  13. Simulations of neutron multiplicity measurements with MCNP-PoliMi.

    SciTech Connect (OSTI)

    Mattingly, John K.; Pozzi, Sara A.; Clarke, Shaun D.; Dennis, Ben D.; Miller, Eric C.

    2010-09-01

    The heightened focus on nuclear safeguards and accountability has increased the need to develop and verify simulation tools for modeling these applications. The ability to accurately simulate safeguards techniques, such as neutron multiplicity counting, aids in the design and development of future systems. This work focuses on validating the ability of the Monte Carlo code MCNPX-PoliMi to reproduce measured neutron multiplicity results for a highly multiplicative sample. The benchmark experiment for this validation consists of a 4.5-kg sphere of plutonium metal that was moderated by various thicknesses of polyethylene. The detector system was the nPod, which contains a bank of 15 3He detectors. Simulations of the experiments were compared to the actual measurements and several sources of potential bias in the simulation were evaluated. The analysis included the effects of detector dead time, source-detector distance, density, and adjustments made to the value of {nu}-bar in the data libraries. Based on this analysis it was observed that a 1.14% decrease in the evaluated value of {nu}-bar for 239Pu in the ENDF-VII library substantially improved the accuracy of the simulation.

  14. Climate Action Champions: Sault Ste. Marie Tribe of Chippewa Indians, MI |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy Sault Ste. Marie Tribe of Chippewa Indians, MI Climate Action Champions: Sault Ste. Marie Tribe of Chippewa Indians, MI The Sault Ste. Marie Tribe of Chippewa Indians is a 44,000-strong federally recognized Indian tribe that is an economic, social and cultural force in its community across the eastern Upper Peninsula counties of Chippewa, Luce, Mackinac, Schoolcraft, Alger, Delta and Marquette, with housing and tribal centers, casinos, and other enterprises that employ

  15. BNL Technical Services Awarded Service-Disabled Veteran-Owned Small

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Business of the Year | Department of Energy BNL Technical Services Awarded Service-Disabled Veteran-Owned Small Business of the Year BNL Technical Services Awarded Service-Disabled Veteran-Owned Small Business of the Year October 1, 2014 - 10:39am Addthis DOE Chief of Staff Kevin Knobloch (l) and DOE OSDBU Director John Hale III (r) present Service-Disabled Veteran-Owned Small Business of the Year to BNL Tech President and Chief Financial Officer Wilson Stevenson and BNL Tech Chief Executive

  16. CubeSats could soon be zooming around space under their own power

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    CubeSats under their own power CubeSats could soon be zooming around space under their own power Los Alamos researchers have created and tested a safe and innovative rocket motor concept that could soon see CubeSats zooming around space and even steering themselves back to Earth when they're finished their mission. November 4, 2016 CubeSats could soon be zooming around space under their own power The Colville River runs across northern Alaska. CubeSats could soon be zooming around space under

  17. Performance Excellence Partners Wins Woman-Owned Small Business of the Year

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Award at Small Business Forum & Expo | Department of Energy Performance Excellence Partners Wins Woman-Owned Small Business of the Year Award at Small Business Forum & Expo Performance Excellence Partners Wins Woman-Owned Small Business of the Year Award at Small Business Forum & Expo October 10, 2014 - 9:52am Addthis Rachel Ramirez, CEO & President of Performance Excellence Partners, accepts the Woman-Owned Small Business of the Year Award from Kevin Knobloch, DOE Chief of

  18. TGLO - Application to Perform Seismic Work on State-Owned Land...

    Open Energy Information (Open El) [EERE & EIA]

    TGLO - Application to Perform Seismic Work on State-Owned Land Jump to: navigation, search OpenEI Reference LibraryAdd to library Form: TGLO - Application to Perform Seismic Work...

  19. May 23 ChallengeHER Women Owned Small Business Event in Washington...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    The campaign is an exciting new initiative that leverages the resources of SBA, Women Impacting Public Policy, and American Express OPEN to promote the Women-Owned Small Business ...

  20. Idaho - IDAPA 20.03.08 - Easements on State-Owned Lands | Open...

    Open Energy Information (Open El) [EERE & EIA]

    20.03.08 - Easements on State-Owned LandsLegal Abstract This regulation outlines the law surrounding the granting of easements on state lands. Published NA Year Signed or Took...

  1. Hammerfest Strom UK co owned by StatoilHydro | Open Energy Information

    Open Energy Information (Open El) [EERE & EIA]

    navigation, search Name: Hammerfest Strom UK co owned by StatoilHydro Address: The Innovation Centre 1 Ainslie Road Hillington Business Park Place: Glasgow Zip: G52 4RU Region:...

  2. Fact #727: May 14, 2012 Nearly Twenty Percent of Households Own Three or More Vehicles

    Office of Energy Efficiency and Renewable Energy (EERE)

    Household vehicle ownership has changed over the last six decades. In 1960, over twenty percent of households did not own a vehicle, but by 2010, that number fell to less than 10%. The number of...

  3. Stories of Discovery & Innovation: Beating Nature at her Own Game? | U.S.

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    DOE Office of Science (SC) Beating Nature at her Own Game? Energy Frontier Research Centers (EFRCs) EFRCs Home Centers Research Science Highlights News & Events EFRC News EFRC Events DOE Announcements Publications History Contact BES Home 08.24.11 Stories of Discovery & Innovation: Beating Nature at her Own Game? Print Text Size: A A A Subscribe FeedbackShare Page New catalyst speeds conversion of electricity to hydrogen fuel. This work, featured in the Office of Science's Stories of

  4. Family-Owned Restaurant Serves Up Huge Energy Savings | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Energy Family-Owned Restaurant Serves Up Huge Energy Savings Family-Owned Restaurant Serves Up Huge Energy Savings May 8, 2013 - 2:27pm Addthis Energy efficiency upgrades helped the Athenian Corner reduce its operating costs and improved the restaurant's bottom line. | Photo courtesy of BetterBuildings Lowell Energy Upgrade program. Energy efficiency upgrades helped the Athenian Corner reduce its operating costs and improved the restaurant's bottom line. | Photo courtesy of BetterBuildings

  5. Purple Bacteria Develops Its Own Form of Sunscreen | U.S. DOE Office of

    Office of Science (SC) [DOE]

    Science (SC) Purple Bacteria Develops Its Own Form of "Sunscreen" Energy Frontier Research Centers (EFRCs) EFRCs Home Centers Research Science Highlights Highlight Archives News & Events Publications History Contact BES Home 05.03.12 Purple Bacteria Develops Its Own Form of "Sunscreen" Print Text Size: A A A FeedbackShare Page Scientific Achievement Found that specific pigments in the light harvesting complex of a photosynthetic bacterium act primarily to protect the

  6. Government-Owned Firearms Inventory at the Nevada National Security Site

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Government-Owned Firearms Inventory at the Nevada National Security Site INS-L-15-01 December 2014 U.S. Department of Energy Office of Inspector General Office of Audits and Inspections Department of Energy Washington, DC 20585 December 3, 2014 MEMORANDUM FOR THE MANAGER, NEVADA FIELD OFFICE FROM: Marilyn E. Richardson Acting Assistant Inspector General for the Office of Inspections Office of Inspector General SUBJECT: INFORMATION: Inspection Report on "Government-Owned Firearms Inventory

  7. miR-196a targets netrin 4 and regulates cell proliferation and migration of cervical cancer cells

    SciTech Connect (OSTI)

    Zhang, Jie; Zheng, Fangxia; Yu, Gang; Yin, Yanhua; Lu, Qingyang

    2013-11-01

    Highlights: miR-196a was overexpressed in cervical cancer tissue compared to normal tissue. miR-196a expression elevated proliferation and migration of cervical cancer cells. miR-196a inhibited NTN4 expression by binding 3?-UTR region of NTN4 mRNA. NTN4 inversely correlated with miR-196a expression in cervical tissue and cell line. NTN4 expression was low in cervical cancer tissue compared to normal tissue. -- Abstract: Recent research has uncovered tumor-suppressive and oncogenic potential of miR-196a in various tumors. However, the expression and mechanism of its function in cervical cancer remains unclear. In this study, we assess relative expression of miR-196a in cervical premalignant lesions, cervical cancer tissues, and four cancer cell lines using quantitative real-time PCR. CaSki and HeLa cells were treated with miR-196a inhibitors, mimics, or pCDNA/miR-196a to investigate the role of miR-196a in cancer cell proliferation and migration. We demonstrated that miR-196a was overexpressed in cervical intraepithelial neoplasia 23 and cervical cancer tissue. Moreover, its expression contributes to the proliferation and migration of cervical cancer cells, whereas inhibiting its expression led to a reduction in proliferation and migration. Five candidate targets of miR-196a chosen by computational prediction and Cervical Cancer Gene Database search were measured for their mRNA in both miR-196a-overexpressing and -depleted cancer cells. Only netrin 4 (NTN4) expression displayed an inverse association with miR-196a. Fluorescent reporter assays revealed that miR-196a inhibited NTN4 expression by targeting one binding site in the 3?-untranslated region (3?-UTR) of NTN4 mRNA. Furthermore, qPCR and Western blot assays verified NTN4 expression was downregulated in cervical cancer tissues compared to normal controls, and in vivo mRNA level of NTN4 inversely correlated with miR-196a expression. In summary, our findings provide new insights about the functional role of

  8. <mi>B> -meson decay constants from 2+1 -flavor lattice QCD with domain-wall light quarks and relativistic heavy quarks

    SciTech Connect (OSTI)

    Christ, N. H.; Flynn, J. M.; Izubuchi, T.; Kawanai, T.; Lehner, C.; Soni, A.; Van de Water, R. S.; Witzel, O.

    2015-03-01

    We calculate the mi>Bmi>-meson decay constants mi>fmi>mi>Bmi>, mi>fmi>mi>Bmi>mi>smi>, and their ratio in unquenched lattice QCD using domain-wall light quarks and relativistic mi>b> quarks. We use gauge-field ensembles generated by the RBC and UKQCD collaborations using the domain-wall fermion action and Iwasaki gauge action with three flavors of light dynamical quarks. We analyze data at two lattice spacings of mi>ami>0.11, 0.086 fm with unitary pion masses as light as mi>Mmi>mi>πmi>290mi>MeV>; this enables us to control the extrapolation to the physical light-quark masses and continuum. For the mi>b> quarks we use the anisotropic clover action with the relativistic heavy-quark interpretation, such that discretization errors from the heavy-quark action are of the same size as from the light-quark sector. We renormalize the

  9. miR-214 promotes the proliferation and invasion of osteosarcoma cells through direct suppression of LZTS1

    SciTech Connect (OSTI)

    Xu, Zhengyu; Wang, Tao

    2014-06-27

    Highlights: • miR-214 is upregulated in human OS tissues and inversely correlated with LZTS1 expression. • miR-214 directly targets LZTS1 by binding to its 3′-UTR. • miR-214 promotes OS cell proliferation, invasion and tumor growth. • Overexpression of LZTS1 reverses miR-214-induced proliferation and invasion of OS cells. - Abstract: Previous studies have shown that miR-214 functions either as an oncogene or a tumor suppressor in various human cancer types. The role of this microRNA in osteosarcoma (OS) is presently unclear. Here, we demonstrated that miR-214 is frequently upregulated in OS specimens, compared with noncancerous bone tissues. Bioinformatics analysis further revealed leucine zipper, putative tumor suppressor 1 (LZTS1) as a potential target of miR-214. Expression patterns of miR-214 were inversely correlated with those of LZTS1 mRNA and protein in OS tissues. Data from reporter assays showed that miR-214 directly binds to the 3′-untranslated region (3′-UTR) of LZTS1 mRNA and suppresses expression at both transcriptional and translational levels. In functional assays, miR-214 promoted OS cell proliferation, invasion and tumor growth in nude mice, which could be reversed by overexpression of LZTS1. Taken together, our data provide compelling evidence that miR-214 functions as an onco-miRNA in OS, and its oncogenic effects are mediated chiefly through downregulation of LZTS1.

  10. MiR-132 prohibits proliferation, invasion, migration, and metastasis in breast cancer by targeting HN1

    SciTech Connect (OSTI)

    Zhang, Zhan-Guo Chen, Wei-Xun Wu, Yan-Hui Liang, Hui-Fang Zhang, Bi-Xiang

    2014-11-07

    Highlights: • MiR-132 is down-regulated in breast cancer tissues and cell lines. • MiR-132 directly regulates HN1 by binding its 3′ UTR. • MiR-132 shows regulatory role in proliferation, invasion, migration and metastasis. • HN1 is involved in miR-132-mediated cell behavior. • Aberrant HN1 is associated with worse overall survival of breast cancer patients. - Abstract: Accumulating evidence indicates that miRNAs play critical roles in tumorigenesis and cancer progression. This study aims to investigate the role and the underlying mechanism of miR-132 in breast cancer. Here, we report that miR-132 is significantly down-regulated in breast cancer tissues and cancer cell lines. Additional study identifies HN1 as a novel direct target of miR-132. MiR-132 down-regulates HN1 expression by binding to the 3′ UTR of HN1 transcript, thereby, suppressing multiple oncogenic traits such as cancer cell proliferation, invasion, migration and metastasis in vivo and in vitro. Overexpression of HN1 restores miR-132-suppressed malignancy. Importantly, higher HN1 expression is significantly associated with worse overall survival of breast cancer patients. Taken together, our data demonstrate a critical role of miR-132 in prohibiting cell proliferation, invasion, migration and metastasis in breast cancer through direct suppression of HN1, supporting the potential utility of miR-132 as a novel therapeutic strategy against breast cancer.

  11. miR-421 induces cell proliferation and apoptosis resistance in human nasopharyngeal carcinoma via downregulation of FOXO4

    SciTech Connect (OSTI)

    Chen, Liang; Department of Otolaryngology, Guangzhou General Hospital of PLA Guangzhou Command, Guangzhou 510010 ; Tang, Yanping; Wang, Jian; Yan, Zhongjie; Xu, Ruxiang

    2013-06-14

    Highlights: •miR-421 is upregulated in nasopharyngeal carcinoma. •miR-421 induces cell proliferation and apoptosis resistance. •FOXO4 is a direct and functional target of miR-421. -- Abstract: microRNAs have been demonstrated to play important roles in cancer development and progression. Hence, identifying functional microRNAs and better understanding of the underlying molecular mechanisms would provide new clues for the development of targeted cancer therapies. Herein, we reported that a microRNA, miR-421 played an oncogenic role in nasopharyngeal carcinoma. Upregulation of miR-421 induced, whereas inhibition of miR-421 repressed cell proliferation and apoptosis resistance. Furthermore, we found that upregulation of miR-421 inhibited forkhead box protein O4 (FOXO4) signaling pathway following downregulation of p21, p27, Bim and FASL expression by directly targeting FOXO4 3′UTR. Additionally, we demonstrated that FOXO4 expression is critical for miR-421-induced cell growth and apoptosis resistance. Taken together, our findings not only suggest that miR-421 promotes nasopharyngeal carcinoma cell proliferation and anti-apoptosis, but also uncover a novel regulatory mechanism for inactivation of FOXO4 in nasopharyngeal carcinoma.

  12. Roles of miRNAs in microcystin-LR-induced Sertoli cell toxicity

    SciTech Connect (OSTI)

    Zhou, Yuan; Wang, Hui; Wang, Cong; Qiu, Xuefeng; Benson, Mikael; Yin, Xiaoqin; Xiang, Zou; Li, Dongmei; and others

    2015-08-15

    Microcystin (MC)-LR, a cyclic heptapeptide, is a potent reproductive system toxin. To understand the molecular mechanisms of MC-induced reproductive system cytotoxicity, we evaluated global changes of miRNA and mRNA expression in mouse Sertoli cells following MC-LR treatment. Our results revealed that the exposure to MC-LR resulted in an altered miRNA expression profile that might be responsible for the modulation of mRNA expression. Bio-functional analysis indicated that the altered genes were involved in specific cellular processes, including cell death and proliferation. Target gene analysis suggested that junction injury in Sertoli cells exposed to MC-LR might be mediated by miRNAs through the regulation of the Sertoli cell-Sertoli cell pathway. Collectively, these findings may enhance our understanding on the modes of action of MC-LR on mouse Sertoli cells as well as the molecular mechanisms underlying the toxicity of MC-LR on the male reproductive system. - Highlights: • miRNAs were altered in Sertoli cells exposed to MC-LR. • Alerted genes were involved in different cell functions including the cell morphology. • MC-LR adversely affected Sertoli cell junction formation through the regulating miRNAs.

  13. Financial statistics of major U.S. publicly owned electric utilities 1997

    SciTech Connect (OSTI)

    1998-12-01

    The 1997 edition of the ``Financial Statistics of Major U.S. Publicly Owned Electric Utilities`` publication presents 5 years (1993 through 1997) of summary financial data and current year detailed financial data on the major publicly owned electric utilities. The objective of the publication is to provide Federal and State governments, industry, and the general public with current and historical data that can be used for policymaking and decisionmaking purposes related to publicly owned electric utility issues. Generator (Tables 3 through 11) and nongenerator (Tables 12 through 20) summaries are presented in this publication. Five years of summary financial data are provided (Tables 5 through 11 and 14 through 20). Summaries of generators for fiscal years ending June 30 and December 31, nongenerators for fiscal years ending June 30 and December 31, and summaries of all respondents are provided in Appendix C. The composite tables present aggregates of income statement and balance sheet data, as well as financial indicators. Composite tables also display electric operation and maintenance expenses, electric utility plant, number of consumers, sales of electricity, operating revenue, and electric energy account data. The primary source of publicly owned financial data is the Form EIA-412, ``Annual Report of Public Electric Utilities.`` Public electric utilities file this survey on a fiscal year basis, in conformance with their recordkeeping practices. The EIA undertook a review of the Form EIA-412 submissions to determine if alternative classifications of publicly owned electric utilities would permit the inclusion of all respondents. The review indicated that financial indicators differ most according to whether or not a publicly owned electric utility generates electricity. Therefore, the main body of the report provides summary information in generator/nongenerator classifications. 2 figs., 101 tabs.

  14. Ionizing Radiation–Inducible miR-27b Suppresses Leukemia Proliferation via Targeting Cyclin A2

    SciTech Connect (OSTI)

    Wang, Bo; Li, Dongping; Kovalchuk, Anna; Litvinov, Dmitry; Kovalchuk, Olga

    2014-09-01

    Purpose: Ionizing radiation is a common carcinogen that is important for the development of leukemia. However, the underlying epigenetic mechanisms remain largely unknown. The goal of the study was to explore microRNAome alterations induced by ionizing radiation (IR) in murine thymus, and to determine the role of IR-inducible microRNA (miRNA/miR) in the development of leukemia. Methods and Materials: We used the well-established C57BL/6 mouse model and miRNA microarray profiling to identify miRNAs that are differentially expressed in murine thymus in response to irradiation. TIB152 human leukemia cell line was used to determine the role of estrogen receptor–α (ERα) in miR-27b transcription. The biological effects of ectopic miR-27b on leukemogenesis were measured by western immunoblotting, cell viability, apoptosis, and cell cycle analyses. Results: Here, we have shown that IR triggers the differential expression of miR-27b in murine thymus tissue in a dose-, time- and sex-dependent manner. miR-27b was significantly down-regulated in leukemia cell lines CCL119 and TIB152. Interestingly, ERα was overexpressed in those 2 cell lines, and it was inversely correlated with miR-27b expression. Therefore, we used TIB152 as a model system to determine the role of ERα in miR-27b expression and the contribution of miR-27b to leukemogenesis. β-Estradiol caused a rapid and transient reduction in miR-27b expression reversed by either ERα-neutralizing antibody or ERK1/2 inhibitor. Ectopic expression of miR-27b remarkably suppressed TIB152 cell proliferation, at least in part, by inducing S-phase arrest. In addition, it attenuated the expression of cyclin A2, although it had no effect on the levels of PCNA, PPARγ, CDK2, p21, p27, p-p53, and cleaved caspase-3. Conclusion: Our data reveal that β-estradiol/ERα signaling may contribute to the down-regulation of miR-27b in acute leukemia cell lines through the ERK1/2 pathway, and that miR-27b may function as a tumor

  15. Curcumin inhibits oral squamous cell carcinoma SCC-9 cells proliferation by regulating miR-9 expression

    SciTech Connect (OSTI)

    Xiao, Can; Wang, Lili; Zhu, Lifang; Zhang, Chenping; Zhou, Jianhua

    2014-11-28

    Highlights: • miR-9 expression level was significantly decreased in OSCC tissues. • Curcumin significantly inhibited SCC-9 cells proliferation. • miR-9 mediates the inhibition of SCC-9 proliferation by curcumin. • Curcumin suppresses Wnt/β-catenin signaling in SCC-9 cells. • miR-9 mediates the suppression of Wnt/β-catenin signaling by curcumin. - Abstract: Curcumin, a phytochemical derived from the rhizome of Curcuma longa, has shown anticancer effects against a variety of tumors. In the present study, we investigated the effects of curcumin on the miR-9 expression in oral squamous cell carcinoma (OSCC) and explored the potential relationships between miR-9 and Wnt/β-catenin pathway in curcumin-mediated OSCC inhibition in vitro. As the results shown, the expression levels of miR-9 were significantly lower in clinical OSCC specimens than those in the adjacent non-tumor tissues. Furthermore, our results indicated that curcumin inhibited OSCC cells (SCC-9 cells) proliferation through up-regulating miR-9 expression, and suppressing Wnt/β-catenin signaling by increasing the expression levels of the GSK-3β, phosphorylated GSK-3β and β-catenin, and decreasing the cyclin D1 level. Additionally, the up-regulation of miR-9 by curcumin in SCC-9 cells was significantly inhibited by delivering anti-miR-9 but not control oligonucleotides. Downregulation of miR-9 by anti-miR-9 not only attenuated the growth-suppressive effects of curcumin on SCC-9 cells, but also re-activated Wnt/β-catenin signaling that was inhibited by curcumin. Therefore, our findings would provide a new insight into the use of curcumin against OSCC in future.

  16. miR-339-5p inhibits alcohol-induced brain inflammation through regulating NF-κB pathway

    SciTech Connect (OSTI)

    Zhang, Yu; Wei, Guangkuan; Di, Zhiyong; Zhao, Qingjie

    2014-09-26

    Graphical abstract: - Highlights: • Alcohol upregulates miR-339-5p expression. • miR-339-5p inhibits the NF-kB pathway. • miR-339-5p interacts with and blocks activity of IKK-beat and IKK-epsilon. • miR-339-5p modulates IL-1β, IL-6 and TNF-α. - Abstract: Alcohol-induced neuroinflammation is mediated by the innate immunesystem. Pro-inflammatory responses to alcohol are modulated by miRNAs. The miRNA miR-339-5p has previously been found to be upregulated in alcohol-induced neuroinflammation. However, little has been elucidated on the regulatory functions of this miRNA in alcohol-induced neuroinflammation. We investigated the function of miR-339-5p in alcohol exposed brain tissue and isolated microglial cells using ex vivo and in vitro techniques. Our results show that alcohol induces transcription of miR 339-5p, IL-6, IL-1β and TNF-α in mouse brain tissue and isolated microglial cells by activating NF-κB. Alcohol activation of NF-κB allows for nuclear translocation of the NF-κB subunit p65 and expression of pro-inflammatory mediators. miR-339-5p inhibited expression of these pro-inflammatory factors through the NF-κB pathway by abolishing IKK-β and IKK-ε activity.

  17. miR-208-3p promotes hepatocellular carcinoma cell proliferation and invasion through regulating ARID2 expression

    SciTech Connect (OSTI)

    Yu, Peng; Wu, Dingguo; You, Yu; Sun, Jing; Lu, Lele; Tan, Jiaxing; Bie, Ping

    2015-08-15

    MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at post-transcriptional level. miRNA dysregulation plays a causal role in cancer progression. In this study, miR-208-3p was highly expressed and directly repressed ARID2 expression. As a result, ARID2 expression in hepatocellular carcinoma (HCC) was decreased. In vitro, miR-208-3p down-regulation and ARID2 over-expression elicited similar inhibitory effects on HCC cell proliferation and invasion. In vivo test results revealed that miR-208-3p down-regulation inhibited HCC tumorigenesis in Hep3B cells. Moreover, ARID2 was possibly a downstream element of transforming growth factor beta1 (TGFβ1)/miR-208-3p/ARID2 regulatory pathway. These findings suggested that miR-208-3p up-regulation is associated with HCC cell progression and may provide a new target for liver cancer treatment. - Highlights: • miR-208-3p was highly expressed and directly repressed the expression of ARID2 in HCC. • miR-208-3p contributed to HCC cell progression both in vitro and in vivo. • Over-expression of ARID2 inhibited the HCC cell proliferation and invasion. • Restoration of ARID2 partly reversed the the effect of miR-208-3p down-regulation on HCC cells. • Newly regulatory pathway: miR-208-3p mediated the repression of ARID2 by TGFβ1 in HCC cells.

  18. Beating Nature at her Own Game? | U.S. DOE Office of Science (SC)

    Office of Science (SC) [DOE]

    Beating Nature at her Own Game? News News Home Featured Articles 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 Science Headlines Science Highlights Presentations & Testimony News Archives Communications and Public Affairs Contact Information Office of Science U.S. Department of Energy 1000 Independence Ave., SW Washington, DC 20585 P: (202) 586-5430 08.24.11 Beating Nature at her Own Game? New catalyst speeds conversion of electricity to hydrogen fuel. Print Text Size: A A A

  19. MicroRNAs expression in ox-LDL treated HUVECs: MiR-365 modulates apoptosis and Bcl-2 expression

    SciTech Connect (OSTI)

    Qin, Bing; Xiao, Bo; Liang, Desheng; Xia, Jian; Li, Ye; Yang, Huan

    2011-06-24

    Highlights: {yields} We evaluated the role of miRNAs in ox-LDL induced apoptosis in ECs. {yields} We found 4 up-regulated and 11 down-regulated miRNAs in apoptotic ECs. {yields} Target genes of the dysregulated miRNAs regulate ECs apoptosis and atherosclerosis. {yields} MiR-365 promotes ECs apoptosis via suppressing Bcl-2 expression. {yields} MiR-365 inhibitor alleviates ECs apoptosis induced by ox-LDL. -- Abstract: Endothelial cells (ECs) apoptosis induced by oxidized low-density lipoprotein (ox-LDL) is thought to play a critical role in atherosclerosis. MicroRNAs (miRNAs) are a class of noncoding RNAs that posttranscriptionally regulate the expression of genes involved in diverse cell functions, including differentiation, growth, proliferation, and apoptosis. However, whether miRNAs are associated with ox-LDL induced apoptosis and their effect on ECs is still unknown. Therefore, this study evaluated potential miRNAs and their involvement in ECs apoptosis in response to ox-LDL stimulation. Microarray and qRT-PCR analysis performed on human umbilical vein endothelial cells (HUVECs) exposed to ox-LDL identified 15 differentially expressed (4 up- and 11 down-regulated) miRNAs. Web-based query tools were utilized to predict the target genes of the differentially expressed miRNAs, and the potential target genes were classified into different function categories with the gene ontology (GO) term and KEGG pathway annotation. In particular, bioinformatics analysis suggested that anti-apoptotic protein B-cell CLL/lymphoma 2 (Bcl-2) is a target gene of miR-365, an apoptomir up-regulated by ox-LDL stimulation in HUVECs. We further showed that transfection of miR-365 inhibitor partly restored Bcl-2 expression at both mRNA and protein levels, leading to a reduction of ox-LDL-mediated apoptosis in HUVECs. Taken together, our findings indicate that miRNAs participate in ox-LDL-mediated apoptosis in HUVECs. MiR-365 potentiates ox-LDL-induced ECs apoptosis by regulating the

  20. MiRNA-125a-5p inhibits glioblastoma cell proliferation and promotes cell differentiation by targeting TAZ

    SciTech Connect (OSTI)

    Yuan, Jian; Xiao, Gelei; Peng, Gang; Liu, Dingyang; Wang, Zeyou; Liao, Yiwei; Liu, Qing; Wu, Minghua; Yuan, Xianrui

    2015-02-06

    Highlights: • Expression of miR-125a-5p is inversely correlated with that of TAZ in glioma cells. • MiR-125a-5p represses TAZ expression in glioma cells. • MiR-125a-5p directly targets the 3′ UTR of TAZ mRNA and promotes its degradation. • MiR-125a-5p represses CTGF and survivin via TAZ, and inhibits glioma cell growth. • MiR-125a-5p inhibits the stem cell features of HFU-251 MG cells. - Abstract: Glioblastoma (GBM) is the most lethal brain tumor due to the resistance to conventional therapies, such as radiotherapy and chemotherapy. TAZ, an important mediator of the Hippo pathway, was found to be up-regulated in diverse cancers, including in GBM, and plays important roles in tumor initiation and progression. However, little is known about the regulation of TAZ expression in tumors. In this study, we found that miR-125a-5p is an important regulator of TAZ in glioma cells by directly targeting the TAZ 3′ UTR. MiR-125a-5p levels are inversely correlated with that of TAZ in normal astrocytes and a panel of glioma cell lines. MiR-125a-5p represses the expression of TAZ target genes, including CTGF and survivin, and inhibits cell proliferation and induces the differentiation of GBM cells; whereas over-expression of TAZ rescues the effects of miR-125a-5p. This study revealed a mechanism for TAZ deregulation in glioma cells, and also demonstrated a tumor suppressor role of miR-125a-5p in glioblastoma cells.

  1. The NuMI proton beam at Fermilab successes and challenges

    SciTech Connect (OSTI)

    Childress, S.; /Fermilab

    2008-11-01

    The NuMI beam at Fermilab has delivered over 5 x 10{sup 20} 120 GeV protons to the neutrino production target since the start for MINOS [1] neutrino oscillation experiment operation in 2005. We report on proton beam commissioning and operation status, including successes and challenges with this beam.

  2. DOE Zero Energy Ready Home Case Study: Cobblestone Homes, Midland, MI

    Office of Energy Efficiency and Renewable Energy (EERE)

    Case study of a DOE Zero Energy Ready home in Midland, MI, that scored HERS 49 without PV or HERS 44 with 1.4 kW of PV. The custom home served as a prototype and energy efficiency demonstration...

  3. MiR-18a regulates the proliferation, migration and invasion of human glioblastoma cell by targeting neogenin

    SciTech Connect (OSTI)

    Song, Yichen; Wang, Ping; Zhao, Wei; Yao, Yilong; Liu, Xiaobai; Ma, Jun; Xue, Yixue; Liu, Yunhui

    2014-05-15

    MiR-17-92 cluster has recently been reported as an oncogene in some tumors. However, the association of miR-18a, an important member of this cluster, with glioblastoma remains unknown. Therefore, this study aims to investigate the expression of miR-18a in glioblastoma and its role in biological behavior of U87 and U251 human glioblastoma cell lines. Quantitative RT-PCR results showed that miR-18a was highly expressed in glioblastoma tissues and U87 and U251 cell lines compared with that in human brain tissues and primary normal human astrocytes, and the expression levels were increased along with the rising pathological grades of glioblastoma. Neogenin was identified as the target gene of miR-18a by dual-luciferase reporter assays. RT-PCR and western blot results showed that its expression levels were decreased along with the rising pathological grades of glioblastoma. Inhibition of miR-18a expression was established by transfecting exogenous miR-18a inhibitor into U87 and U251 cells, and its effects on the biological behavior of glioblastoma cells were studied using CCK-8 assay, transwell assay and flow cytometry. Inhibition of miR-18a expression in U87 and U251 cells significantly up-regulated neogenin, and dramatically suppressed the abilities of cell proliferation, migration and invasion, induced cell cycle arrest and promoted cellular apoptosis. Collectively, these results suggest that miR-18a may regulate biological behavior of human glioblastoma cells by targeting neogenin, and miR-18a can serve as a potential target in the treatment of glioblastoma. - Highlights: • MiR-18a was highly expressed in glioblastoma tissues and U87 and U251 cell lines. • Neogenin was identified as the target gene of miR-18a. • Neogenin expressions were decreased along with the rising pathological grades of glioblastoma. • Inhibition of miR-18a suppressed biological behavior of glioma cells by up-regulating neogenin.

  4. miR-7 and miR-218 epigenetically control tumor suppressor genes RASSF1A and Claudin-6 by targeting HoxB3 in breast cancer

    SciTech Connect (OSTI)

    Li, Qiaoyan; Zhu, Fufan; Chen, Puxiang

    2012-07-20

    Highlights: Black-Right-Pointing-Pointer Both miR-7 and miR-218 down-regulates HoxB3 expression by targeting the 3 Prime -UTR of HoxB3 mRNA. Black-Right-Pointing-Pointer A reverse correlation between the levels of endogenous miR-7, miR218 and HoxB3 expression. Black-Right-Pointing-Pointer Epigenetic changes involve in the reactivation of HoxB3. Black-Right-Pointing-Pointer Both miRNAs inhibits the cell cycle and clone formation of breast cancer cells. -- Abstract: Many microRNAs have been implicated as key regulators of cellular growth and differentiation and have been found to dysregulate proliferation in human tumors, including breast cancer. Cancer-linked microRNAs also alter the epigenetic landscape by way of DNA methylation and post-translational modifications of histones. Aberrations in Hox gene expression are important for oncogene or tumor suppressor during abnormal development and malignancy. Although recent studies suggest that HoxB3 is critical in breast cancer, the putative role(s) of microRNAs impinging on HoxB3 is not yet fully understood. In this study, we found that the expression levels of miR-7 and miR-218 were strongly and reversely associated with HoxB3 expression. Stable overexpression of miR-7 and miR-218 was accompanied by reactivation of tumor suppressor genes including RASSF1A and Claudin-6 by means of epigenetic switches in DNA methylation and histone modification, giving rise to inhibition of the cell cycle and clone formation of breast cancer cells. The current study provides a novel link between overexpression of collinear Hox genes and multiple microRNAs in human breast malignancy.

  5. 90 MW build/own/operate gas turbine combined cycle cogeneration project with sludge drying plant

    SciTech Connect (OSTI)

    Schroppe, J.T.

    1986-04-01

    This paper will discuss some of the unique aspects of a build/own/operate cogeneration project for an oil refinery in which Foster Wheeler is involved. The organization is constructing a 90 MW plant that will supply 55 MW and 160,000 lb/hr of 600 psi, 700F steam to the Tosco Corporation's 130,000 bd Avon Oil Refinery in Martinez, California. (The refinery is located about 45 miles northeast of San Francisco.) Surplus power production will be sold to the local utility, Pacific Gas and Electric Co. (PG and E). Many of the aspects of this project took on a different perspective, since the contractor would build, own and operate the plant.

  6. Loss of expression of miR-335 is implicated in hepatic stellate cell migration and activation

    SciTech Connect (OSTI)

    Chen, Chao; Wu, Chao-Qun; Zhang, Zong-Qi; Yao, Ding-Kang; Zhu, Liang

    2011-07-15

    Activation and migration of resident stellate cells (HSCs) within the hepatic space of Disse play an important role in hepatic fibrosis, which accounts for the increased numbers of activated HSCs in areas of inflammation during hepatic fibrosis. Currently, microRNAs have been found to play essential roles in HSC differentiation, proliferation, apoptosis, fat accumulation and collagen production. However, little is known about microRNA mediated HSC activation and migration. In this study, the miRNA expression profiles of quiescent HSCs, partially activated HSCs and fully activated HSCs were compared in pairs. Gene ontology (GO) and GO-Map network analysis indicated that the activation of HSCs was regulated by microRNAs. Among them miR-335 was confirmed to be significantly reduced during HSC activation by qRT-PCR, and restoring expression of miR-335 inhibited HSC migration and reduced {alpha}-SMA and collagen type I. Previous study revealed that tenascin-C (TNC), an extracellular matrix glycoprotein involved in cell migration, might be a target of miR-335. Therefore, we further studied the TNC expression in miR-335 over-expressed HSCs. Our data showed that exogenous TNC could enhance HSC migration in vitro and miR-335 restoration resulted in a significant inhibition of TNC expression. These results demonstrated that miR-335 restoration inhibited HSC migration, at least in part, via downregulating the TNC expression.

  7. NREL Reports Examine Economic Trade-offs of Owning versus Leasing a Solar

    U.S. Department of Energy (DOE) all webpages (Extended Search)

    Photovoltaic System - News Releases | NREL Reports Examine Economic Trade-offs of Owning versus Leasing a Solar Photovoltaic System January 21, 2015 Two new reports from the Energy Department's National Renewable Energy Laboratory (NREL) examine the economic options customers face when deciding how to finance commercial or residential solar energy systems. NREL analysts found that businesses that use low-cost financing to purchase a photovoltaic (PV) system and homeowners who use

  8. Financial statistics of major U.S. publicly owned electric utilities 1995

    SciTech Connect (OSTI)

    1997-07-01

    The 1995 Edition of the Financial Statistics of Major U.S. Publicly Owned Electric Utilities publication presents 5 years (1991 through 1995) of summary financial data and current year detailed financial data on the major publicly owned electric utilities. The objective of the publication is to provide Federal and State governments, industry, and the general public with current and historical data that can be used for policymaking and decisionmaking purposes related to publicly owned electric utility issues. Generator (Tables 3 through 11) and nongenerator (Tables 12 through 20) summaries are presented in this publication. Five years of summary financial data are provided (Tables 5 through 11 and 14 through 20). Summaries of generators for fiscal years ending June 30 and December 31, nongenerators for fiscal years ending June 30 and December 31, and summaries of all respondents are provided in Appendix C. The composite tables present aggregates of income statement and balance sheet data, as well as financial indicators. Composite tables also display electric operation and maintenance expenses, electric utility plant, number of consumers, sales of electricity, and operating revenue, and electric energy account data. 9 figs., 87 tabs.

  9. PSMB4 promotes multiple myeloma cell growth by activating NF-κB-miR-21 signaling

    SciTech Connect (OSTI)

    Zheng, Peihao; Guo, Honggang; Li, Guangchao; Han, Siqi; Luo, Fei; Liu, Yi

    2015-03-06

    Proteasomal subunit PSMB4, was recently identified as potential cancer driver genes in several tumors. However, the regulatory mechanism of PSMB4 on carcinogenesis process remains unclear. In this study, we investigated the expression and roles of PSMB4 in multiple myeloma (MM). We found a significant up-regulation of PSMB4 in MM plasma and cell lines. Ectopic overexpression of PSMB4 promoted cell growth and colony forming ability of MM cells, whereas inhibition of PSMB4 led to a decrease of such events. Furthermore, our results demonstrated the up-regulation of miR-21 and a positive correlation between the levels of miR-21 and PSMB4 in MM. Re-expression of miR-21 markedly rescued PSMB4 knockdown-mediated suppression of cell proliferation and clone-formation. Additionally, while enforced expression of PSMB4 profoundly increased NF-κB activity and the level of miR-21, PSMB4 knockdown or NF-κB inhibition suppressed miR-21 expression in MM cells. Taken together, our results demonstrated that PSMB4 regulated MM cell growth in part by activating NF-κB-miR-21 signaling, which may represent promising targets for novel specific therapies. - Highlights: • First reported upregulation of PSMB4 in MM plasma and cell lines. • PSMB4 promoted MM cell growth and colony forming ability. • Further found miR-21 was up-regulated by PSMB4 in MM plasma and cell lines. • PSMB4-induced miR-21 expression was modulated by NF-κB. • PSMB4-NF-κB-miR-21 axis may be potential therapeutic targets of MM.

  10. RLIP76-dependent suppression of PI3K/AKT/Bcl-2 pathway by miR...

    Office of Scientific and Technical Information (OSTI)

    in prostate cancer Citation Details In-Document Search Title: RLIP76-dependent suppression of PI3KAKTBcl-2 pathway by miR-101 induces apoptosis in prostate cancer MicroRNA-101 ...

  11. Aryl hydrocarbon receptor-dependent regulation of miR-196a expression controls lung fibroblast apoptosis but not proliferation

    SciTech Connect (OSTI)

    Hecht, Emelia; Zago, Michela; Sarill, Miles; Rico de Souza, Angela; Gomez, Alvin; Matthews, Jason; Hamid, Qutayba; Eidelman, David H.; Baglole, Carolyn J.

    2014-11-01

    The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor implicated in the regulation of apoptosis and proliferation. Although activation of the AhR by xenobiotics such as dioxin inhibits the cell cycle and control apoptosis, paradoxically, AhR expression also promotes cell proliferation and survival independent of exogenous ligands. The microRNA (miRNA) miR-196a has also emerged as a regulator of proliferation and apoptosis but a relationship between the AhR and miR-196a is not known. Therefore, we hypothesized that AhR-dependent regulation of endogenous miR-196a expression would promote cell survival and proliferation. Utilizing lung fibroblasts from AhR deficient (AhR{sup −/−}) and wild-type (AhR{sup +/+}) mice, we show that there is ligand-independent regulation of miRNA, including low miR-196a in AhR{sup −/−} cells. Validation by qRT-PCR revealed a significant decrease in basal expression of miR-196a in AhR{sup −/−} compared to AhR{sup +/+} cells. Exposure to AhR agonists benzo[a]pyrene (B[a]P) and FICZ as well as AhR antagonist CH-223191 decreased miR-196a expression in AhR{sup +/+} fibroblasts concomitant with decreased AhR protein levels. There was increased proliferation only in AhR{sup +/+} lung fibroblasts in response to serum, corresponding to a decrease in p27{sup KIP1} protein, a cyclin-dependent kinase inhibitor. Increasing the cellular levels of miR-196a had no effect on proliferation or expression of p27{sup KIP1} in AhR{sup −/−} fibroblasts but attenuated cigarette smoke-induced apoptosis. This study provides the first evidence that AhR expression is essential for the physiological regulation of cellular miRNA levels- including miR-196a. Future experiments designed to elucidate the functional relationship between the AhR and miR-196a may delineate additional novel ligand-independent roles for the AhR. - Highlights: • The AhR controls proliferation and apoptosis in lung cells. • The AhR regulates the

  12. Material Activation Benchmark Experiments at the NuMI Hadron Absorber Hall in Fermilab

    SciTech Connect (OSTI)

    Matsumura, H.; Matsuda, N.; Kasugai, Y.; Toyoda, A.; Yashima, H.; Sekimoto, S.; Iwase, H.; Oishi, K.; Sakamoto, Y.; Nakashima, H.; Leveling, A.; Boehnlein, D.; Lauten, G.; Mokhov, N.; Vaziri, K.

    2014-06-15

    In our previous study, double and mirror symmetric activation peaks found for Al and Au arranged spatially on the back of the Hadron absorber of the NuMI beamline in Fermilab were considerably higher than those expected purely from muon-induced reactions. From material activation bench-mark experiments, we conclude that this activation is due to hadrons with energy greater than 3 GeV that had passed downstream through small gaps in the hadron absorber.

  13. Measurement of Pi-K Ratios from the NuMI Target

    SciTech Connect (OSTI)

    Seun, Sin Man

    2007-07-01

    Interactions of protons (p) with the NuMI (Neutrinos at the Main Injector) target are used to create the neutrino beam for the MINOS (Main Injector Neutrino Oscillation Search) Experiment. Using the MIPP (Main Injector Particle Production) experimental apparatus, the production of charged pions and kaons in p+NuMI interactions is studied. The data come from a sample of 2 x 106 events obtained by MIPP using the 120 GeV/c proton beam from the Main Injector at Fermi National Accelerator Laboratory in Illinois, USA. Pions and kaons are identified by measurement in a Ring Imaging Cherenkov detector. Presented are measurements of π-+, K-/K+, π+/K+ and π-/K- production ratios in the momentum range pT < 2 GeV/c transversely and 20 GeV/c < pz < 90 GeV/c longitudinally. Also provided are detailed comparisons of the MIPP NuMI data with the MIPP Thin Carbon data, the MIPP Monte Carlo simulation and the current MINOS models in the relevant momentum ranges.

  14. miR-21 modulates tumor outgrowth induced by human adipose tissue-derived mesenchymal stem cells in vivo

    SciTech Connect (OSTI)

    Shin, Keun Koo; Lee, Ae Lim; Kim, Jee Young; Medical Research Center for Ischemic Tissue Engineering, Pusan National University, Yangsan, Gyeongnam 626-870; BK21 Medical Science Education Center, School of Medicine, Pusan National University, Yangsan, Gyeongnam 626-870 ; Lee, Sun Young; Medical Research Center for Ischemic Tissue Engineering, Pusan National University, Yangsan, Gyeongnam 626-870 ; Bae, Yong Chan; Jung, Jin Sup

    2012-06-15

    Highlights: Black-Right-Pointing-Pointer miR-21 modulates hADSC-induced increase of tumor growth. Black-Right-Pointing-Pointer The action is mostly mediated by the modulation of TGF-{beta} signaling. Black-Right-Pointing-Pointer Inhibition of miR-21 enhances the blood flow recovery in hindlimb ischemia. -- Abstract: Mesenchymal stem cells (MSCs) have generated a great deal of interest in clinical situations, due principally to their potential use in regenerative medicine and tissue engineering applications. However, the therapeutic application of MSCs remains limited, unless the favorable effects of MSCs on tumor growth in vivo, and the long-term safety of the clinical applications of MSCs, can be more thoroughly understood. In this study, we determined whether microRNAs can modulate MSC-induced tumor outgrowth in BALB/c nude mice. Overexpression of miR-21 in human adipose-derived stem cells (hADSCs) inhibited hADSC-induced tumor growth, and inhibition of miR-21 increased it. Downregulation of transforming growth factor beta receptor II (TGFBR2), but not of signal transducer and activator of transcription 3, in hADSCs showed effects similar to those of miR-21 overexpression. Downregulation of TGFBR2 and overexpression of miR21 decreased tumor vascularity. Inhibition of miR-21 and the addition of TGF-{beta} increased the levels of vascular endothelial growth factor and interleukin-6 in hADSCs. Transplantation of miR-21 inhibitor-transfected hADSCs increased blood flow recovery in a hind limb ischemia model of nude mice, compared with transplantation of control oligo-transfected cells. These findings indicate that MSCs might favor tumor growth in vivo. Thus, it is necessary to study the long-term safety of this technique before MSCs can be used as therapeutic tools in regenerative medicine and tissue engineering.

  15. Targeting miR-21 enhances the sensitivity of human colon cancer HT-29 cells to chemoradiotherapy in vitro

    SciTech Connect (OSTI)

    Deng, Jun; Lei, Wan; Fu, Jian-Chun; Zhang, Ling; Li, Jun-He; Xiong, Jian-Ping

    2014-01-17

    Highlight: MiR-21 plays a significant role in 5-FU resistance. This role might be attributed to targeting of hMSH2 as well as TP and DPD via miR-21 targeted hMSH2. Indirectly targeted TP and DPD to influence 5-FU chemotherapy sensitivity. -- Abstract: 5-Fluorouracil (5-FU) is a classic chemotherapeutic drug that has been widely used for colorectal cancer treatment, but colorectal cancer cells are often resistant to primary or acquired 5-FU therapy. Several studies have shown that miR-21 is significantly elevated in colorectal cancer. This suggests that this miRNA might play a role in this resistance. In this study, we investigated this possibility and the possible mechanism underlying this role. We showed that forced expression of miR-21 significantly inhibited apoptosis, enhanced cell proliferation, invasion, and colony formation ability, promoted G1/S cell cycle transition and increased the resistance of tumor cells to 5-FU and X radiation in HT-29 colon cancer cells. Furthermore, knockdown of miR-21 reversed these effects on HT-29 cells and increased the sensitivity of HT-29/5-FU to 5-FU chemotherapy. Finally, we showed that miR-21 targeted the human mutS homolog2 (hMSH2), and indirectly regulated the expression of thymidine phosphorylase (TP) and dihydropyrimidine dehydrogenase (DPD). These results demonstrate that miR-21 may play an important role in the 5-FU resistance of colon cancer cells.

  16. Evaluation of codisposal viability of MOX (FFTF) DOE-owned fuel: Phase 1 -- Intact mode calculations

    SciTech Connect (OSTI)

    Goluoglu, S.; Davis, J.W.; Montierth, L.M.

    1999-07-01

    The authors provide the intact criticality information that supports the disposal of spent nuclear fuel (SNF) from the US Department of Energy's (DOE's) Fast Flux Test Facility (FFTF) in the potential Monitored Geologic Repository at Yucca Mountain. FFTF is one of more than 250 forms of DOE-owned SNF. Because of the variety of the DOE SNF, the National Spent Nuclear Fuel Program has designated nine representative fuel groups for disposal criticality analyses based on fuel matrix, primary fissile isotope, and enrichment. The FFTF fuel is representative of the mixed-oxide fuel (MOX) group.

  17. To Own or Lease Solar: Understanding Commercial Retailers' Decisions to Use Alternative Financing Models

    SciTech Connect (OSTI)

    Feldman, David; Margolis, Robert

    2014-12-01

    This report examines the tradeoffs among financing methods for businesses installing onsite photovoltaics (PV). We present case studies of PV financing strategies used by two large commercial retailers that have deployed substantial U.S. PV capacity: IKEA, which owns its PV, and Staples, which purchases power generated from onsite PV systems through power purchase agreements (PPAs). We also analyze the financial considerations that influence any company's choice of PV financing strategy. Our goal in this report is to clarify the financial and institutional costs and benefits of financing strategies and to inform other companies that are considering launching or expanding similar PV programs.

  18. Interconnection Standards

    Energy.gov [DOE]

    California's "Rule 21" generally applies to systems connecting to an investor-owned utility’s distribution grid, non-export generating facilities connecting to an investor-owned utility’s transmi...

  19. Table 3. Top five retailers of electricity, with end use sectors...

    U.S. Energy Information Administration (EIA) (indexed site)

    4,"Wheeling Power Co","Investor-owned",3269892,425005,447849,2397038,0 5,"Black Diamond Power Co","Investor-owned",56232,39152,17080,0,0 " ","Total sales, top five ...

  20. Table 3. Top five retailers of electricity, with end use sectors...

    U.S. Energy Information Administration (EIA) (indexed site)

    2,"Southwestern Public Service Co","Investor-owned",5014741,1127323,1655334,2232084,0 3,"El Paso Electric Co","Investor-owned",1640996,651515,910681,78800,0 4,"City of Farmington - ...

  1. miR-502 inhibits cell proliferation and tumor growth in hepatocellular carcinoma through suppressing phosphoinositide 3-kinase catalytic subunit gamma

    SciTech Connect (OSTI)

    Chen, Suling; Li, Fang; Chai, Haiyun; Tao, Xin; Wang, Haili; Ji, Aifang

    2015-08-21

    MicroRNAs (miRNAs) play a key role in carcinogenesis and tumor progression in hepatocellular carcinoma (HCC). In the present study, we demonstrated that miR-502 significantly inhibits HCC cell proliferation in vitro and tumor growth in vivo. G1/S cell cycle arrest and apoptosis of HCC cells were induced by miR-502. Phosphoinositide 3-kinase catalytic subunit gamma (PIK3CG) was identified as a direct downstream target of miR-502 in HCC cells. Notably, overexpression of PIK3CG reversed the inhibitory effects of miR-502 in HCC cells. Our findings suggest that miR-502 functions as a tumor suppressor in HCC via inhibition of PI3KCG, supporting its utility as a promising therapeutic gene target for this tumor type. - Highlights: • miR-502 suppresses HCC cell proliferation in vitro and tumorigenicity in vivo. • miR-502 regulates cell cycle and apoptosis in HCC cells. • PIK3CG is a direct target of miR-502. • miR-502 and PIK3CG expression patterns are inversely correlated in HCC tissues.

  2. RLIP76-dependent suppression of PI3K/AKT/Bcl-2 pathway by miR-101 induces apoptosis in prostate cancer

    SciTech Connect (OSTI)

    Yang, Jing; Song, Qi; Cai, Yi; Wang, Peng; Wang, Min; Zhang, Dong

    2015-08-07

    MicroRNA-101 (miR-101) participates in carcinogenesis and tumor progression in various cancers. However, its biological functions in prostate cancer are still unclear. Here, we demonstrate that miR-101 represents a critical role in regulating cell apoptosis in prostate cancer cells. We first demonstrated that miR-101 treatment promoted apoptosis in DU145 and PC3 cells by using flow cytometric analysis and transmission electron microscopy (TEM). To verify the mechanisms, we identified a novel miR-101 target, Ral binding protein 1 (RLIP76). We found miR-101 transfection significantly suppresses RLIP76 expression, which can transactivate phosphorylation of PI3K-Akt signaling, and resulted in an amplification of Bcl2-induced apoptosis. Furthermore, we demonstrated that RLIP76 overexpression could reverse the anti-tumor effects of miR-101 in DU145 and PC3 cells by using flow cytometry assay and MTT assay. Taken together, our results revealed that the effect of miR-101 on prostate cancer cell apoptosis was due to RLIP76 regulation of the PI3K/Akt/Bcl-2 signaling pathway. - Highlights: • miR-101 inhibited prostate cancer cell proliferation and enhanced apoptosis. • miR-101 directly targeted and regulated RLIP76 expression. • miR-101 suppressed PI3K/Akt/Bcl-2 signaling pathway by targeting RLIP76.

  3. A spacecraft's own ambient environment: The role of simulation-based research

    SciTech Connect (OSTI)

    Ketsdever, Andrew D.; Gimelshein, Sergey

    2014-12-09

    Spacecraft contamination has long been a subject of study in the rarefied gas dynamics community. Professor Mikhail Ivanov coined the term a spacecraft's 'own ambient environment' to describe the effects of natural and satellite driven processes on the conditions encountered by a spacecraft in orbit. Outgassing, thruster firings, and gas and liquid dumps all contribute to the spacecraft's contamination environment. Rarefied gas dynamic modeling techniques, such as Direct Simulation Monte Carlo, are well suited to investigate these spacebased environments. However, many advances were necessary to fully characterize the extent of this problem. A better understanding of modeling flows over large pressure ranges, for example hybrid continuum and rarefied numerical schemes, were required. Two-phase flow modeling under rarefied conditions was necessary. And the ability to model plasma flows for a new era of propulsion systems was also required. Through the work of Professor Ivanov and his team, we now have a better understanding of processes that create a spacecraft's own ambient environment and are able to better characterize these environments. Advances in numerical simulation have also spurred on the development of experimental facilities to study these effects. The relationship between numerical results and experimental advances will be explored in this manuscript.

  4. MiR-20a Induces Cell Radioresistance by Activating the PTEN/PI3K/Akt Signaling Pathway in Hepatocellular Carcinoma

    SciTech Connect (OSTI)

    Zhang, Yuqin; Zheng, Lin; Ding, Yi; Li, Qi; Wang, Rong; Liu, Tongxin; Sun, Quanquan; Yang, Hua; Peng, Shunli; Wang, Wei; Chen, Longhua

    2015-08-01

    Purpose: To investigate the role of miR-20a in hepatocellular carcinoma (HCC) cell radioresistance, which may reveal potential strategies to improve treatment. Methods and Materials: The expression of miR-20a and PTEN were detected in HCC cell lines and paired primary tissues by quantitative real-time polymerase chain reaction. Cell radiation combined with colony formation assays was administrated to discover the effect of miR-20a on radiosensitivity. Bioinformatics prediction and luciferase assay were used to identify the target of miR-20a. The phosphatidylinositol 3-kinase inhibitor LY294002 was used to inhibit phosphorylation of Akt, to verify whether miR-20a affects HCC cell radioresistance through activating the PTEN/PI3K/Akt pathway. Results: MiR-20a levels were increased in HCC cell lines and tissues, whereas PTEN was inversely correlated with it. Overexpression of miR-20a in Bel-7402 and SMMC-7721 cells enhances their resistance to the effect of ionizing radiation, and the inhibition of miR-20a in HCCLM3 and QGY-7701 cells sensitizes them to it. PTEN was identified as a direct functional target of miR-20a for the induction of radioresistance. Overexpression of miR-20a activated the PTEN/PI3K/Akt signaling pathway. Additionally, the kinase inhibitor LY294002 could reverse the effect of miR-20a–induced radioresistance. Conclusion: MiR-20a induces HCC cell radioresistance by activating the PTEN/PI3K/Akt pathway, which suggests that miR-20a/PTEN/PI3K/Akt might represent a target of investigation for developing effective therapeutic strategies against HCC.

  5. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Standard Eligible Technologies: Eligibility: Investor-Owned Utility, Local Government, Retail Supplier Savings Category: Geothermal Electric, Solar Thermal Electric, Solar...

  6. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Alternative Energy Portfolio Standard Background Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Geothermal Electric, Solar Thermal Electric, Solar...

  7. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    alternative retail suppliers, electric cooperativ... Eligibility: Investor-Owned Utility, Municipal Utilities, Cooperative Utilities, Retail Supplier Savings Category:...

  8. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Digestion Alternative Energy Portfolio Standard Background Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Geothermal Electric, Solar Thermal Electric,...

  9. Electric Power Board of Chattanooga - Energy Efficient New Homes...

    Energy.gov (indexed) [DOE]

    Construction Investor-Owned Utility Municipal Utilities Residential Cooperative Utilities Multifamily Residential Savings Category Comprehensive MeasuresWhole Building Program...

  10. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    of Hyde... Eligibility: Commercial, Industrial, Investor-Owned Utility, Local Government, Nonprofit, Municipal Utilities, Residential, Cooperative Utilities, Schools, State...

  11. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Renewable Energy and Energy Efficiency Portfolio Standard Eligible Technologies Eligibility: Investor-Owned Utility, Municipal Utilities, Cooperative Utilities Savings Category:...

  12. List of Photovoltaics Incentives | Open Energy Information

    Open Energy Information (Open El) [EERE & EIA]

    Standard West Virginia Investor-Owned Utility Retail Supplier Anaerobic Digestion Biodiesel Biomass CHPCogeneration Fuel Cells Geothermal Electric Hydroelectric energy...

  13. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    energy, photovoltaics, dedicated crops grown for energy production and organic waste biomass, hydropower th... Eligibility: Commercial, Industrial, Investor-Owned...

  14. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    reimburs... Eligibility: Commercial, Construction, Industrial, Investor-Owned Utility, Local Government, Municipal Utilities, Cooperative Utilities, State Government, Federal...

  15. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  16. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    several other types o... Eligibility: Commercial, Industrial, Investor-Owned Utility, Local Government, Nonprofit, Municipal Utilities, Residential, Cooperative Utilities,...

  17. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    the end of 2011. In... Eligibility: Commercial, Industrial, Investor-Owned Utility, Local Government, Nonprofit, Municipal Utilities, Residential, Cooperative Utilities,...

  18. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Renewable Fuels, Other Distributed Generation Technologies, Microturbines Alternative Energy Portfolio Standard Background Eligibility: Investor-Owned Utility, Retail Supplier...

  19. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Standard Eligible Technologies Eligibility: Investor-Owned Utility, Local Government, Municipal Utilities, Cooperative Utilities Savings Category: Solar Water Heat, Geothermal...

  20. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    other types o... Eligibility: Commercial, Industrial, Investor-Owned Utility, Local Government, Nonprofit, Municipal Utilities, Residential, Cooperative Utilities, Schools,...

  1. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Eligible Technologies Eligibility: Investor-Owned Utility, Local Government, Municipal Utilities, Cooperative Utilities Savings Category: Solar Water Heat, Geothermal Electric,...

  2. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    repeals the Sustainably Priced Energy Enterprise Development program's renewable energy goals. The Renewable... Eligibility: Investor-Owned Utility, Municipal Utilities,...

  3. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Building Insulation Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned...

  4. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Generation Technologies Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned...

  5. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Dehumidifiers Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility,...

  6. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Advanced Energy Fund History Eligibility: Commercial, Industrial, Investor-Owned Utility, Municipal Utilities, Residential, Cooperative Utilities, Institutional Savings Category:...

  7. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Renewables Portfolio Standard Eligible Technologies: Eligibility: Investor-Owned Utility, Local Government,...

  8. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Renewables Portfolio Standard Eligible Technologies: Eligibility: Investor-Owned Utility, Local Government, Retail Supplier Savings Category: Geothermal Electric, Solar Thermal...

  9. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Requirements for Wind Development Decommissioning Eligibility: Commercial, Construction, Industrial, Investor-Owned Utility, Municipal Utilities, Cooperative Utilities, Installers...

  10. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Investor-Owned Utility, Municipal Utilities, Cooperative Utilities Savings Category: Solar Photovoltaics, Wind (All), Biomass, Hydroelectric, Municipal Solid Waste, Wind...

  11. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    charge (or several other types o... Eligibility: Commercial, Industrial, Investor-Owned Utility, Local Government, Nonprofit, Municipal Utilities, Residential, Cooperative...

  12. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Renewable Portfolio Standard Eligible Technologies Eligibility: Investor-Owned Utility, Local Government, Municipal Utilities, Cooperative Utilities Savings Category: Solar Water...

  13. Other Policy | Open Energy Information

    Open Energy Information (Open El) [EERE & EIA]

    Incentive Type Place Applicable Sector Eligible Technologies Active Alternative Energy Portfolio Standard (Massachusetts) Other Policy Massachusetts Investor-Owned Utility...

  14. MiR-145 is downregulated in human ovarian cancer and modulates cell growth and invasion by targeting p70S6K1 and MUC1

    SciTech Connect (OSTI)

    Wu, Huijuan; Xiao, ZhengHua; Wang, Ke; Liu, Wenxin; Hao, Quan

    2013-11-29

    Highlights: •MiR-145 is downregulated in human ovarian cancer. •MiR-145 targets p70S6K1 and MUC1. •p70S6K1 and MUC1 are involved in miR-145 mediated tumor cell growth and cell invasion, respectively. -- Abstract: MicroRNAs (miRNAs) are a family of small non-coding RNA molecules that regulate gene expression at post-transcriptional levels. Previous studies have shown that miR-145 is downregulated in human ovarian cancer; however, the roles of miR-145 in ovarian cancer growth and invasion have not been fully demonstrated. In the present study, Northern blot and qRT-PCR analysis indicate that miR-145 is downregulated in ovarian cancer tissues and cell lines, as well as in serum samples of ovarian cancer, compared to healthy ovarian tissues, cell lines and serum samples. Functional studies suggest that miR-145 overexpression leads to the inhibition of colony formation, cell proliferation, cell growth viability and invasion, and the induction of cell apoptosis. In accordance with the effect of miR-145 on cell growth, miR-145 suppresses tumor growth in vivo. MiR-145 is found to negatively regulate P70S6K1 and MUC1 protein levels by directly targeting their 3′UTRs. Importantly, the overexpression of p70S6K1 and MUC1 can restore the cell colony formation and invasion abilities that are reduced by miR-145, respectively. MiR-145 expression is increased after 5-aza-CdR treatment, and 5-aza-CdR treatment results in the same phenotype as the effect of miR-145 overexpression. Our study suggests that miR-145 modulates ovarian cancer growth and invasion by suppressing p70S6K1 and MUC1, functioning as a tumor suppressor. Moreover, our data imply that miR-145 has potential as a miRNA-based therapeutic target for ovarian cancer.

  15. UTILITY CHARACTERISTICS",,,,,,,"RESIDENTIAL",,,"COMMERCIAL",...

    U.S. Energy Information Administration (EIA) (indexed site)

    ... 2015,1,59624,"Vivint Solar, Inc.","MD","Behind the ... 2015,1,9324,"Indiana Michigan Power Co","MI","Investor ...

  16. UTILITY CHARACTERISTICS ",,,,,,,"RESIDENTIAL",,,"COMMERCIAL...

    U.S. Energy Information Administration (EIA) (indexed site)

    ... 2016,1,59624,"Vivint Solar, Inc.","MD","Behind the ... 2016,1,9324,"Indiana Michigan Power Co","MI","Investor ...

  17. The long non-coding RNA H19-derived miR-675 modulates human gastric cancer cell proliferation by targeting tumor suppressor RUNX1

    SciTech Connect (OSTI)

    Zhuang, Ming; Gao, Wen; Xu, Jing; Wang, Ping; Shu, Yongqian

    2014-06-06

    Graphical abstract: - Highlights: • H19 regulates gastric cancer cell proliferation phenotype via miR-675. • MiR-675 modulates cell proliferation of gastric cancer cells by targeting tumor suppressor RUNX1. • The H19/miR-675/RUNX1 axis plays an important role in the tumorigenesis of gastric cancer. - Abstract: The lncRNA H19 has been recently shown to be upregulated and play important roles in gastric cancer tumorigenesis. However, the precise molecular mechanism of H19 and its mature product miR-675 in the carcinogenesis of gastric cancer remains unclear. In this study, we found that miR-675 was positively expressed with H19 and was a pivotal mediator in H19-induced gastric cancer cell growth promotion. Subsequently, the tumor suppressor Runt Domain Transcription Factor1 (RUNX1) was confirmed to be a direct target of miR-675 using a luciferase reporter assay and Western blotting analyses. A series of rescue assays indicated that RUNX1 mediated H19/miR-67-induced gastric cancer cell phenotypic changes. Moreover, the inverse relationship between the expression of RUNX1 and H19/miR-675 was also revealed in gastric cancer tissues and gastric cancer cell lines. Taken together, our study demonstrated that the novel pathway H19/miR-675/RUNX1 regulates gastric cancer development and may serve as a potential target for gastric cancer therapy.

  18. Testing CPT conservation using the NuMI neutrino beam with the MINOS experiment

    SciTech Connect (OSTI)

    Auty, David John

    2010-05-01

    The MINOS experiment was designed to measure neutrino oscillation parameters with muon neutrinos. It achieves this by measuring the neutrino energy spectrum and flavor composition of the man-made NuMI neutrino beam 1km after the beam is formed and again after 735 km. By comparing the two spectra it is possible to measure the oscillation parameters. The NuMI beam is made up of 7.0% {bar {nu}}{sub {mu}}, which can be separated from the {nu}{sub {mu}} because the MINOS detectors are magnetized. This makes it possible to study {bar {nu}}{sub {mu}} oscillations separately from those of muon neutrinos, and thereby test CPT invariance in the neutrino sector by determining the {bar {nu}}{sub {mu}} oscillation parameters and comparing them with those for {nu}{sub {mu}}, although any unknown physics of the antineutrino would appear as a difference in oscillation parameters. Such a test has not been performed with beam {bar {nu}}{sub {mu}} before. It is also possible to produce an almost pure {bar {nu}}{sub {mu}} beam by reversing the current through the magnetic focusing horns of the NuMI beamline, thereby focusing negatively, instead of positively charged particles. This thesis describes the analysis of the 7% {bar {nu}}{sub {mu}} component of the forward horn current NuMI beam. The {bar {nu}}{sub {mu}} of a data sample of 3.2 x 10{sup 20} protons on target analysis found 42 events, compared to a CPT conserving prediction of 58.3{sub -7.6}{sup +7.6}(stat.){sub -3.6}{sup +3.6}(syst.) events. This corresponds to a 1.9 {sigma} deficit, and a best fit value of {Delta}{bar m}{sub 32}{sup 2} = 18 x 10{sup -3} eV{sup 2} and sin{sup 2} 2{bar {theta}}{sub 23} = 0.55. This thesis focuses particularly on the selection of {bar {nu}}{sub {mu}} events, and investigates possible improvements of the selection algorithm. From this a different selector was chosen, which corroborated the findings of the original selector. The thesis also investigates how the systematic errors affect the

  19. ,"Detroit, MI Natural Gas Pipeline Imports From Canada (MMcf)"

    U.S. Energy Information Administration (EIA) (indexed site)

    Imports From Canada (MMcf)" ,"Click worksheet name or tab at bottom for data" ,"Worksheet Name","Description","# Of Series","Frequency","Latest Data for" ,"Data 1","Detroit, MI Natural Gas Pipeline Imports From Canada (MMcf)",1,"Annual",2015 ,"Release Date:","10/31/2016" ,"Next Release Date:","11/30/2016" ,"Excel File

  20. ,"Marysville, MI Natural Gas Pipeline Imports From Canada (MMcf)"

    U.S. Energy Information Administration (EIA) (indexed site)

    Imports From Canada (MMcf)" ,"Click worksheet name or tab at bottom for data" ,"Worksheet Name","Description","# Of Series","Frequency","Latest Data for" ,"Data 1","Marysville, MI Natural Gas Pipeline Imports From Canada (MMcf)",1,"Annual",2015 ,"Release Date:","10/31/2016" ,"Next Release Date:","11/30/2016" ,"Excel File

  1. Port Huron, MI Natural Gas Pipeline Imports From Canada (Dollars per

    U.S. Energy Information Administration (EIA) (indexed site)

    Thousand Cubic Feet) Dollars per Thousand Cubic Feet) Port Huron, MI Natural Gas Pipeline Imports From Canada (Dollars per Thousand Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2016 2.07 2.06 2.21 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 10/31/2016 Next Release Date: 11/30/2016 Referring Pages: U.S. Price of

  2. ,"St. Clair, MI Natural Gas Pipeline Exports to Canada (MMcf)"

    U.S. Energy Information Administration (EIA) (indexed site)

    Exports to Canada (MMcf)" ,"Click worksheet name or tab at bottom for data" ,"Worksheet Name","Description","# Of Series","Frequency","Latest Data for" ,"Data 1","St. Clair, MI Natural Gas Pipeline Exports to Canada (MMcf)",1,"Annual",2015 ,"Release Date:","10/31/2016" ,"Next Release Date:","11/30/2016" ,"Excel File

  3. ,"St. Clair, MI Natural Gas Pipeline Imports From Canada (MMcf)"

    U.S. Energy Information Administration (EIA) (indexed site)

    Imports From Canada (MMcf)" ,"Click worksheet name or tab at bottom for data" ,"Worksheet Name","Description","# Of Series","Frequency","Latest Data for" ,"Data 1","St. Clair, MI Natural Gas Pipeline Imports From Canada (MMcf)",1,"Annual",2015 ,"Release Date:","10/31/2016" ,"Next Release Date:","11/30/2016" ,"Excel File

  4. Who Owns Renewable Energy Certificates? An Exploration of PolicyOptions and Practice

    SciTech Connect (OSTI)

    Holt, Edward A.; Wiser, Ryan; Bolinger, Mark

    2006-04-05

    Renewable energy certificates (RECs) represent the bundle of information that describes the characteristics of renewable electricity generation, and may be (and increasingly are) sold separately from the underlying electricity itself. RECs are a relatively new phenomenon, emerging as a tradable commodity in voluntary markets in the late 1990s, and gaining strength as a means of compliance with various state policy requirements affecting renewable generation in the early 2000s (Holt and Bird 2005). Twenty states and Washington, D.C. now have mandatory renewables portfolio standard (RPS) obligations, and most of these may be satisfied by owning and retiring RECs. Many states also have fuel source and emissions disclosure requirements, for which RECs are useful. Even where state policy does not allow unbundled and fully tradable RECs to meet these requirements, RECs may still be used as an accounting and verification tool (REC tracking systems are in place or under development in many regions of the U.S.). These applications, plus REC trading activity in support of voluntary green claims, give rise to potential ''double counting'' to the extent that the purchaser of the RECs and the purchaser of the underlying electricity both make claims to the renewable energy attributes of the facility in question (Hamrin and Wingate 2003). When renewable electricity is sold and purchased, an important question therefore arises: ''Who owns the RECs created by the generation of renewable energy?'' In voluntary transactions, most agree that the question of REC ownership can and should be negotiated between the buyer and the seller privately, and should be clearly established by contract. Claims about purchasing renewable energy should only be made if REC ownership can be documented. In many other cases, however, renewable energy transactions are either mandated or encouraged through state or federal policy. In these cases, the issue of REC ownership must often be answered by

  5. Assessment of radiological releases from the NuMI facility during MINOS and NOvA operations

    SciTech Connect (OSTI)

    Martens, Mike; /Fermilab

    2007-04-01

    This report makes projections of the radiological releases from the NuMI facility during operations for the MINOS and NO ?A experiments. It includes an estimate of the radionuclide levels released into the atmosphere and the estimated tritium and sodium-22 concentrations in the NuMI sump water and Fermilab pond system. The analysis was performed for NuMI operations with a beam power on target increased from the present 400 kW design up to a possible 1500 kW with future upgrades. The total number of protons on target was assumed to be 18 x 10{sup 20} after the completion of MINOS and 78 x 10{sup 20} after the completion of NO ?A.

  6. Identification of miR-2400 gene as a novel regulator in skeletal muscle satellite cells proliferation by targeting MYOG gene

    SciTech Connect (OSTI)

    Zhang, Wei Wei; Tong, Hui Li; Sun, Xiao Feng; Hu, Qian; Yang, Yu; Li, Shu Feng; Yan, Yun Qin; Li, Guang Peng

    2015-08-07

    MicroRNAs play critical roles in skeletal muscle development as well as in regulation of muscle cell proliferation and differentiation. Previous study in our laboratory showed that the expression level of miR-2400, a novel and unique miRNA from bovine, had significantly changed in skeletal muscle-derived satellite cells (MDSCs) during differentiation, however, the function and expression pattern for miR-2400 in MDSCs has not been fully understood. In this report, we firstly identified that the expression levels of miR-2400 were down-regulated during MDSCs differentiation by stem-loop RT-PCR. Over-expression and inhibition studies demonstrated that miR-2400 promoted MDSCs proliferation by EdU (5-ethynyl-2′ deoxyuridine) incorporation assay and immunofluorescence staining of Proliferating cell nuclear antigen (PCNA). Luciferase reporter assays showed that miR-2400 directly targeted the 3′ untranslated regions (UTRs) of myogenin (MYOG) mRNA. These data suggested that miR-2400 could promote MDSCs proliferation through targeting MYOG. Furthermore, we found that miR-2400, which was located within the eighth intron of the Wolf-Hirschhorn syndrome candidate 1-like 1 (WHSC1L1) gene, was down-regulated in MDSCs in a direct correlation with the WHSC1L1 transcript by Clustered regularly interspaced palindromic repeats interference (CRISPRi). In addition, these observations not only provided supporting evidence for the codependent expression of intronic miRNAs and their host genes in vitro, but also gave insight into the role of miR-2400 in MDSCs proliferation. - Highlights: • miR-2400 is a novel and unique miRNA from bovine. • miR-2400 could promote skeletal muscle satellite cells proliferation. • miR-2400 directly targeted the 3′ untranslated regions of MYOG mRNA. • miR-2400 could be coexpressed together with its host gene WHSC1L1.

  7. miR-340 inhibits glioblastoma cell proliferation by suppressing CDK6, cyclin-D1 and cyclin-D2

    SciTech Connect (OSTI)

    Li, Xuesong; Gong, Xuhai; Chen, Jing; Zhang, Jinghui; Sun, Jiahang; Guo, Mian

    2015-05-08

    Glioblastoma development is often associated with alteration in the activity and expression of cell cycle regulators, such as cyclin-dependent kinases (CKDs) and cyclins, resulting in aberrant cell proliferation. Recent studies have highlighted the pivotal roles of miRNAs in controlling the development and growth of glioblastoma. Here, we provide evidence for a function of miR-340 in the inhibition of glioblastoma cell proliferation. We found that miR-340 is downregulated in human glioblastoma tissue samples and several established glioblastoma cell lines. Proliferation and neurosphere formation assays revealed that miR-340 plays an oncosuppressive role in glioblastoma, and that its ectopic expression causes significant defect in glioblastoma cell growth. Further, using bioinformatics, luciferase assay and western blot, we found that miR-340 specifically targets the 3′UTRs of CDK6, cyclin-D1 and cyclin-D2, leading to the arrest of glioblastoma cells in the G0/G1 cell cycle phase. Confirming these results, we found that re-introducing CDK6, cyclin-D1 or cyclin-D2 expression partially, but significantly, rescues cells from the suppression of cell proliferation and cell cycle arrest mediated by miR-340. Collectively, our results demonstrate that miR-340 plays a tumor-suppressive role in glioblastoma and may be useful as a diagnostic biomarker and/or a therapeutic avenue for glioblastoma. - Highlights: • miR-340 is downregulated in glioblastoma samples and cell lines. • miR-340 inhibits glioblastoma cell proliferation. • miR-340 directly targets CDK6, cyclin-D1, and cyclin-D2. • miR-340 regulates glioblastoma cell proliferation via CDK6, cyclin-D1 and cyclin-D2.

  8. Electric Trade in the United States

    Reports and Publications

    1998-01-01

    Final Issue. Presents information on bulk power transactions by investor-owned utilities, federal and other publicly-owned utilities, and cooperative utilities.

  9. Evaluation of codisposal viability of MOX (FFTF) DOE-owned fuel: Phase 2 -- Degraded mode calculations

    SciTech Connect (OSTI)

    Goluoglu, S.; Angers, L.; Davis, J.W.; Stockman, H.; Gottlieb, P.; Montierth, L.M.

    1999-07-01

    The authors provide the degraded criticality information that supports the disposal of spent nuclear fuel (SNF) from the US Department of Energy's (DOE's) Fast Flux Test facility (FFTF) in the potential Monitored Geologic Repository (MGR) at Yucca Mountain. FFTF is one of more than 250 forms of DOE-owned SNF. Because of the variety of the SNF, the National Spent Nuclear Fuel Program has designated nine representative fuel groups for disposal criticality analyses based on fuel matrix, primary fissile isotope, and enrichment. The FFTF fuel is a mixture of uranium and plutonium oxides and is representative of the mixed-oxide fuel (MOX) group. The analyses were performed according to the disposal criticality analysis methodology that was documented in the topical report submitted to the US nuclear Regulatory Commission (YMP/TR-004Q). The methodology includes analyzing the geochemical and physical processes that can breach the waste package and degrade the waste forms. This paper summarizes the results of geochemistry degradation analysis and the criticality calculations using the degradation products.

  10. Transcript abundance on its own cannot be used to infer fluxes in central metabolism

    SciTech Connect (OSTI)

    Schwender, Jorg; Konig, Christina; Klapperstuck, Matthias; Heinzel, Nicolas; Munz, Eberhard; Hebbelmann, Inga; Hay, Jordan O.; Denolf, Peter; De Bodt, Stefanie; Redestig, Henning; Caestecker, Evelyne; Jakob, Peter M.; Borisjuk, Ljudmilla; Rolletschek, Hardy

    2014-11-28

    An attempt has been made to define the extent to which metabolic flux in central plant metabolism is reflected by changes in the transcriptome and metabolome, based on an analysis of in vitro cultured immature embryos of two oilseed rape (Brassica napus) accessions which contrast for seed lipid accumulation. Metabolic flux analysis (MFA) was used to constrain a flux balance metabolic model which included 671 biochemical and transport reactions within the central metabolism. This highly confident flux information was eventually used for comparative analysis of flux vs. transcript (metabolite). Metabolite profiling succeeded in identifying 79 intermediates within the central metabolism, some of which differed quantitatively between the two accessions and displayed a significant shift corresponding to flux. An RNA-Seq based transcriptome analysis revealed a large number of genes which were differentially transcribed in the two accessions, including some enzymes/proteins active in major metabolic pathways. With a few exceptions, differential activity in the major pathways (glycolysis, TCA cycle, amino acid, and fatty acid synthesis) was not reflected in contrasting abundances of the relevant transcripts. The conclusion was that transcript abundance on its own cannot be used to infer metabolic activity/fluxes in central plant metabolism. Lastly, this limitation needs to be borne in mind in evaluating transcriptome data and designing metabolic engineering experiments.

  11. State-owned companies dominate list of largest non-U. S. producers

    SciTech Connect (OSTI)

    Beck, R.J.; Williamson, M.

    1994-09-05

    Because state-owned oil and gas companies dominate Oil and Gas Journal's list of largest non-US producers, data aren't fully comparable with those of the OGJ300. Many state companies report only production and reserves, with little or no financial data. Companies on the OGJ100, therefore, cannot be ranked by assets or revenues. Instead, they are listed by regions, based on location of corporate headquarters. There was no change in makeup of the top 20 holders of crude oil reserves. These companies' reserves totaled 872.3 billion bbl in 1993. The top 20 non-US companies now control 87.3 % of total world crude oil reserves, according to OGJ estimates. This is up marginally from 87.2 % of total world oil reserves in 1992. The top 20 had 87.7 % of total world reserves in 1991 and 85.5 % in 1990. The table lists company name, total assets, revenues, net income, capital and exploratory expenditures, worldwide oil production, gas production, oil and gas reserves worldwide.

  12. Facilitating communities in designing and using their own community health impact assessment tool

    SciTech Connect (OSTI)

    Cameron, Colleen; Ghosh, Sebanti; Eaton, Susan L.

    2011-07-15

    Reducing health inequities and improving the health of communities require an informed public that is aware of the social determinants of health and how policies and programs have an impact on the health of their communities. People Assessing Their Health (PATH) is a process that uses community-driven health impact assessment to build the capacity of people to become active participants in the decisions that affect the well-being of their community. The PATH process is both a health promotion and a community development approach that builds people's ability to bring critical analysis to a situation and to engage in effective social action to bring about desired change. Because it increases analytical skills and provides communities with their own unique tool to assess the potential impact of projects, programs or policies on the health and well-being of their community it is an empowering process. PATH was originally used in three communities in northeastern Nova Scotia, Canada in 1996 when the Canadian health care system was being restructured to a more decentralized system. Since then it has been used in other communities in Nova Scotia and India. This paper will describe the PATH process and the use of the community health impact assessment as well as the methodology used in the PATH process. The lessons learned from PATH's experiences of building capacity among the community in Canada and India will be presented.

  13. White Paper: Multi-purpose canister (MPC) for DOE-owned spent nuclear fuel (SNF)

    SciTech Connect (OSTI)

    Knecht, D.A.

    1994-04-01

    The paper examines the issue, What are the advantages, disadvantages, and other considerations for using the MPC concept as part of the strategy for interim storage and disposal of DOE-owned SNF? The paper is based in part on the results of an evaluation made for the DOE National Spent Fuel Program by the Waste Form Barrier/Canister Team, which is composed of knowledgeable DOE and DOE-contractor personnel. The paper reviews the MPC and DOE SNF status, provides criteria and other considerations applicable to the issue, and presents an evaluation, conclusions, and recommendations. The primary conclusion is that while most of DOE SNF is not currently sufficiently characterized to be sealed into an MPC, the advantages of standardized packages in handling, reduced radiation exposure, and improved human factors should be considered in DOE SNF program planning. While the design of MPCs for DOE SNF are likely premature at this time, the use of canisters should be considered which are consistent with interim storage options and the MPC design envelope.

  14. Transcript abundance on its own cannot be used to infer fluxes in central metabolism

    DOE PAGES-Beta [OSTI]

    Schwender, Jorg; Konig, Christina; Klapperstuck, Matthias; Heinzel, Nicolas; Munz, Eberhard; Hebbelmann, Inga; Hay, Jordan O.; Denolf, Peter; De Bodt, Stefanie; Redestig, Henning; et al

    2014-11-28

    An attempt has been made to define the extent to which metabolic flux in central plant metabolism is reflected by changes in the transcriptome and metabolome, based on an analysis of in vitro cultured immature embryos of two oilseed rape (Brassica napus) accessions which contrast for seed lipid accumulation. Metabolic flux analysis (MFA) was used to constrain a flux balance metabolic model which included 671 biochemical and transport reactions within the central metabolism. This highly confident flux information was eventually used for comparative analysis of flux vs. transcript (metabolite). Metabolite profiling succeeded in identifying 79 intermediates within the central metabolism,more » some of which differed quantitatively between the two accessions and displayed a significant shift corresponding to flux. An RNA-Seq based transcriptome analysis revealed a large number of genes which were differentially transcribed in the two accessions, including some enzymes/proteins active in major metabolic pathways. With a few exceptions, differential activity in the major pathways (glycolysis, TCA cycle, amino acid, and fatty acid synthesis) was not reflected in contrasting abundances of the relevant transcripts. The conclusion was that transcript abundance on its own cannot be used to infer metabolic activity/fluxes in central plant metabolism. Lastly, this limitation needs to be borne in mind in evaluating transcriptome data and designing metabolic engineering experiments.« less

  15. ,"Sault St Marie, MI Natural Gas Pipeline Exports to Canada (Million Cubic Feet)"

    U.S. Energy Information Administration (EIA) (indexed site)

    Sault St Marie, MI Natural Gas Pipeline Exports to Canada (Million Cubic Feet)" ,"Click worksheet name or tab at bottom for data" ,"Worksheet Name","Description","# Of Series","Frequency","Latest Data for" ,"Data 1","Sault St Marie, MI Natural Gas Pipeline Exports to Canada (Million Cubic Feet)",1,"Annual",2015 ,"Release Date:","10/31/2016" ,"Next Release

  16. Do financial investors destabilize the oil price?

    Annual Energy Outlook

    Telephone +49 69 1344 0 Internet http:www.ecb.europa.eu Fax +49 69 1344 6000 All rights ... More speci...cally, all other things equal, futures prices will be moved by the ow of ...

  17. Do financial investors destabilize the oil price?

    Gasoline and Diesel Fuel Update

    and Commercial Consumers by Local Distributio Area: District of Columbia Florida Georgia Maryland Michigan New Jersey New York Ohio Pennsylvania Virginia Period: Monthly Annual Download Series History Download Series History Definitions, Sources & Notes Definitions, Sources & Notes Show Data By: Data Series Area 2010 2011 2012 2013 2014 2015 View History Residential Average Price 13.53 13.06 12.10 12.45 13.05 11.98 1980-2015 Commercial Average Price 12.26 12.24 11.19 11.64 12.18

  18. miR-206 is down-regulated in breast cancer and inhibits cell proliferation through the up-regulation of cyclinD2

    SciTech Connect (OSTI)

    Zhou, Jing; Tian, Ye; Li, Juan; Lu, Binbin; Sun, Ming; Zou, Yanfen; Kong, Rong; Luo, Yanhong; Shi, Yongguo; Wang, Keming; Ji, Guozhong

    2013-04-05

    Highlights: ? miR-206 was downexpressed in tumor samples compared with matched normal samples. ? Enhanced expression of miR-206 could inhibit breast cancer growth in vitro. ? Luciferase confirmed miR-206 functions as an anti-oncogene by targeting cyclinD2. ? A reverse correlation between miR-206 and cyclinD2 in breast cancer was found. -- Abstract: MicroRNAs act as important gene regulators in human genomes, and their aberrant expression is linked to many malignancies. Aberrant expression of miR-206 has been frequently reported in cancer studies; however, the role and mechanism of its function in breast cancer remains unclear. Quantitative real-time PCR was performed to detect the relative expression levels of miR-206 in breast cancer and normal breast tissues. Lower expression of miR-206 in breast cancer tissues was associated with larger tumour size and a more advanced clinical stage. Further in vitro observations showed that the enforced expression of miR-206 in MCF-7 breast cancer cells inhibited cell growth by blocking the G1/S transition and suppressed cell proliferation and colony formation, implying that miR-206 functions as a tumour suppressor in the progression of breast cancer. Interestingly, Luciferase assays first revealed that miR-206 inhibited cyclinD2 expression by targeting two binding sites in the 3?-untranslated region of cyclinD2 mRNA. qRT-PCR and Western blot assays verified that miR-206 reduced cyclinD2 expression at both the mRNA and protein levels. A reverse correlation between miR-206 and cyclinD2 expression was noted in breast cancer tissues. Altogether, our results identify a crucial tumour suppressive role of miR-206 in the progression of breast cancer, at least partly via up-regulation of the expression of cyclinD2, and suggest that miR-206 might be a candidate prognostic predictor or an anticancer therapeutic target for breast cancer patients.

  19. MiR-138 promotes smooth muscle cells proliferation and migration in db/db mice through down-regulation of SIRT1

    SciTech Connect (OSTI)

    Xu, Juan; Li, Li; Yun, Hui-fang; Han, Ye-shan

    2015-08-07

    Background: Diabetic vascular smooth muscle cells (VSMCs) exhibit significantly increased rates of proliferation and migration, which was the most common pathological change in atherosclerosis. In addition, the study about the role for miRNAs in the regulation of VSMC proliferation is just beginning to emerge and additional miRNAs involved in VSMC proliferation modulation should be identified. Methods: The expression of miR-138 and SIRT1 were examined in SMCs separated from db/db mice and in SMC lines C-12511 exposed to high glucose with qRT-PCR and western blot. The regulation of miR-138 on the expression of SMCs was detected with luciferase report assay. VSMCs proliferation and migration assays were performed to examine the effect of miR-138 inhibitor on VSMCs proliferation and migration. Results: We discovered that higher mRNA level of miR-138 and reduced expression of SIRT1 were observed in SMCs separated from db/db mice and in SMC lines C-12511. Moreover, luciferase report assay showed that the activity of SIRT1 3′-UTR was highly increased by miR-138 inhibitor and reduced by miR-138 mimic. In addition, we examined that the up-regulation of NF-κB induced by high glucose in SMCs was reversed by resveratrol and miR-138 inhibitor. MTT and migration assays showed that miR-138 inhibitor attenuated the proliferation and migration of smooth muscle cells. Conclusion: In this study, we revealed that miR-138 might promote proliferation and migration of SMC in db/db mice through suppressing the expression of SIRT1. - Highlights: • Higher mRNA level of miR-138 was observed in SMCs from db/db mice. • The mRNA and protein level of SIRT1 in SMCs from db/db mice were greatly reduced. • miR-138 could regulate the expression of SIRT1 in SMCs. • SIRT1 overexpression reversed the up-regulation of acetylized p65 and NF-κB induced by high glucose. • MiR-138 inhibitor reversed VSMCs proliferation and migration induced by high glucose.

  20. miR-15a/16 Enhances Radiation Sensitivity of Non-Small Cell Lung Cancer Cells by Targeting the TLR1/NF-κB Signaling Pathway

    SciTech Connect (OSTI)

    Lan, Fengming; Yue, Xiao; Ren, Gang; Li, Hongqi; Ping, Li; Wang, Yingjie; Xia, Tingyi

    2015-01-01

    Purpose: Many miRNAs have been identified as essential issues and core determining factors in tumor radiation. Recent reports have demonstrated that miRNAs and Toll-like receptors could exert reciprocal effects to control cancer development in various ways. However, a novel role of miR-15a/16 in enhancing radiation sensitivity by directly targeting TLR1 has not been reported, to our knowledge. Methods and Materials: Bioinformatic analyses, luciferase reporter assay, biochemical assays, and subcutaneous tumor establishment were used to characterize the signaling pathways of miRNA-15a/16 in response to radiation treatment. Results: First, an inverse correlation between the expression of miR-15a/16 and TLR1 protein was revealed in non-small cell lung cancer (NSCLC) and normal lung tissues. Next, we corroborated that miR-15a/16 specifically bound to TLR1 3′UTR and inhibited the expression of TLR1 in H358 and A549 cells. Furthermore, miR-15a/16 downregulated the activity of the NF-κB signaling pathway through TLR1. In addition, overexpression of miR-15a/16 inhibited survival capability and increased radiation-induced apoptosis, resulting in enhancement of radiosensitivity in H358 and A549 cells. Finally, subcutaneous tumor bearing NSCLC cells in a nude mice model was established, and the results showed that combined groups (miR-15a/16 + radiation) inhibited tumor growth more significantly than did radiation alone. Conclusions: We mainly elucidate that miRNA-15a/16 can enhance radiation sensitivity by regulating the TLR1/NF-κB signaling pathway and act as a potential therapeutic approach to overcome radioresistance for lung cancer treatment.

  1. St. Clair, MI Natural Gas Pipeline Imports From Canada (Million Cubic Feet)

    U.S. Energy Information Administration (EIA) (indexed site)

    Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1990's 14,132 11,855 34,592 33,388 2000's 17,198 21,747 28,441 5,202 22,853 18,281 10,410 9,633 9,104 6,544 2010's 5,591 5,228 3,531 6,019 16,409 9,024 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 10/31/2016 Next Release Date: 11/30/2016 Referring Pages: U.S. Natural Gas Pipeline Imports by Point of Entry St. Clair, MI Natural

  2. Observation of Disappearance of Muon Neutrinos in the NuMI Beam

    SciTech Connect (OSTI)

    Pavlovic, Zarko

    2008-05-01

    The Main Injector Neutrino Oscillation Search (MINOS) is a two detector long-baseline neutrino experiment designed to study the disappearance of muon neutrinos. MINOS will test the vμ → vτ oscillation hypothesis and measure precisely Δm232 and sin223 oscillation parameters. The source of neutrinos for MINOS experiment is Fermilab's Neutrinos at the Main Injector (NuMI) beamline. The energy spectrum and the composition of the beam is measured at two locations, one close to the source and the other 735 km down-stream in the Soudan Mine Underground Laboratory in northern Minnesota. The precision measurement of the oscillation parameters requires an accurate prediction of the neutrino flux at the Far Detector. This thesis discusses the calculation of the neutrino flux at the Far Detector and its uncertainties. A technique that uses the Near Detector data to constrain the uncertainties in the calculation of the flux is described. The data corresponding to an exposure of 2.5 x 1020 protons on the NuMI target is presented and an energy dependent disappearance pattern predicted by neutrino oscillation hypotheses is observed in the Far Detector data. The fit to MINOS data, for given exposure, yields the best fit values for Δm$2\\atop{23}$ and sin223 to be (2.38$+0.20\\atop{-0.16}$) x 10-3 eV2/c4and 1.00-0.08, respectively.

  3. Validation of the MCNPX-PoliMi Code to Design a Fast-Neutron Multiplicity Counter

    SciTech Connect (OSTI)

    J. L. Dolan; A. C. Kaplan; M. Flaska; S. A. Pozzi; D. L. Chichester

    2012-07-01

    Many safeguards measurement systems used at nuclear facilities, both domestically and internationally, rely on He-3 detectors and well established mathematical equations to interpret coincidence and multiplicity-type measurements for verifying quantities of special nuclear material. Due to resource shortages alternatives to these existing He-3 based systems are being sought. Work is also underway to broaden the capabilities of these types of measurement systems in order to improve current multiplicity analysis techniques. As a part of a Material Protection, Accounting, and Control Technology (MPACT) project within the U.S. Department of Energy's Fuel Cycle Technology Program we are designing a fast-neutron multiplicity counter with organic liquid scintillators to quantify important quantities such as plutonium mass. We are also examining the potential benefits of using fast-neutron detectors for multiplicity analysis of advanced fuels in comparison with He-3 detectors and testing the performance of such designs. The designs are being developed and optimized using the MCNPX-PoliMi transport code to study detector response. In the full paper, we will discuss validation measurements used to justify the use of the MCNPX-PoliMi code paired with the MPPost multiplicity routine to design a fast neutron multiplicity counter with liquid scintillators. This multiplicity counter will be designed with the end goal of safeguarding advanced nuclear fuels. With improved timing qualities associated with liquid scintillation detectors, we can design a system that is less limited by nuclear materials of high activities. Initial testing of the designed system with nuclear fuels will take place at Idaho National Laboratory in a later stage of this collaboration.

  4. T-1025 IU SciBath-768 detector tests in MI-12

    SciTech Connect (OSTI)

    Tayloe, Rex; Cooper, R.; Garrison, L.; Thornton, T.; Rebenitsch, L.; DeJongh, Fritz; Loer, Benjamin; Ramberg, Erik; Yoo, Jonghee; /Fermilab

    2012-02-11

    This is a memorandum of understanding between the Fermi National Accelerator Laboratory (Fermilab) and the experimenters of Department of Physics and Center for Exploration of Energy and Matter, Indiana University, who have committed to participate in detector tests to be carried out during the 2012 Fermilab Neutrino program. The memorandum is intended solely for the purpose of recording expectations for budget estimates and work allocations for Fermilab, the funding agencies and the participating institutions. it reflects an arrangement that currently is satisfactory to the parties; however, it is recognized and anticipated that changing circumstances of the evolving research program will necessitate revisions. The parties agree to modify this memorandum to reflect such required adjustments. Actual contractual obligations will be set forth in separate documents. The experimenters propsoe to test their prototype 'SciBat-768' detector in the MI-12 building for 3 months (February-April) in Spring 2012. The major goal of this effort is to measure or limit the flux of beam-induced neutrons in a far-off-axis (> 45{sup o}) location of the Booster Neutrino Beamline (BNB). This flux is of interest for a proposed coherent neutral-current neutrino-argon elastic scattering experiment. A second goal is to collect more test data for the SciBath-768 to enable better understanding and calibration of the device. The SciBath-768 detector successfully ran for 3 months in the MINOS Underground Area in Fall 2011 as testbeam experiment T-1014 and is currently running above ground in the MINOS service building. For the run proposed here, the experiments are requesting: space in MI-12 in which to run the SciBath detector during February-April 2012 while the BNB is operating; technical support to help with moving the equipment on site; access to power, internet, and accelerator signals; and a small office space from which to run and monitor the experiment.

  5. The multiple market-exposure of waste management companies: A case study of two Swedish municipally owned companies

    SciTech Connect (OSTI)

    Corvellec, Herve; Bramryd, Torleif

    2012-09-15

    Highlights: Black-Right-Pointing-Pointer Swedish municipally owned waste management companies are active on political, material, technical, and commercial markets. Black-Right-Pointing-Pointer These markets differ in kind and their demands follow different logics. Black-Right-Pointing-Pointer These markets affect the public service, processing, and marketing of Swedish waste management. Black-Right-Pointing-Pointer Articulating these markets is a strategic challenge for Swedish municipally owned waste management. - Abstract: This paper describes how the business model of two leading Swedish municipally owned solid waste management companies exposes them to four different but related markets: a political market in which their legitimacy as an organization is determined; a waste-as-material market that determines their access to waste as a process input; a technical market in which these companies choose what waste processing technique to use; and a commercial market in which they market their products. Each of these markets has a logic of its own. Managing these logics and articulating the interrelationships between these markets is a key strategic challenge for these companies.

  6. Memorandum requesting a clarification of the circumstances under which a DOE Government Owned Contractor Operated (GOCO) facility

    Energy.gov [DOE]

    Memorandum requesting a clarification of the circumstances under which a DOE Government Owned Contractor Operated (GOCO) facility may be considered a laser manufacturer and subject to FDA laser manufacturer requirements and other points of interpretation of the FDA Exemption Letter, 78EL-01DOE (DOE exemption or exemption) by the LSSG for GOCG facilities.

  7. Actual Versus Estimated Utility Factor of a Large Set of Privately Owned Chevrolet Volts

    SciTech Connect (OSTI)

    John Smart; Thomas Bradley; Stephen Schey

    2014-04-01

    In order to determine the overall fuel economy of a plug-in hybrid electric vehicle (PHEV), the amount of operation in charge depleting (CD) versus charge sustaining modes must be determined. Mode of operation is predominantly dependent on customer usage of the vehicle and is therefore highly variable. The utility factor (UF) concept was developed to quantify the distance a group of vehicles has traveled or may travel in CD mode. SAE J2841 presents a UF calculation method based on data collected from travel surveys of conventional vehicles. UF estimates have been used in a variety of areas, including the calculation of window sticker fuel economy, policy decisions, and vehicle design determination. The EV Project, a plug-in electric vehicle charging infrastructure demonstration being conducted across the United States, provides the opportunity to determine the real-world UF of a large group of privately owned Chevrolet Volt extended range electric vehicles. Using data collected from Volts enrolled in The EV Project, this paper compares the real-world UF of two groups of Chevrolet Volts to estimated UF's based on J2841. The actual observed fleet utility factors (FUF) for the MY2011/2012 and MY2013 Volt groups studied were observed to be 72% and 74%, respectively. Using the EPA CD ranges, the method prescribed by J2841 estimates a FUF of 65% and 68% for the MY2011/2012 and MY2013 Volt groups, respectively. Volt drivers achieved higher percentages of distance traveled in EV mode for two reasons. First, they had fewer long-distance travel days than drivers in the national travel survey referenced by J2841. Second, they charged more frequently than the J2841 assumption of once per day - drivers of Volts in this study averaged over 1.4 charging events per day. Although actual CD range varied widely as driving conditions varied, the average CD ranges for the two Volt groups studied matched the EPA CD range estimates, so CD range variation did not affect FUF results.

  8. MiR-520b suppresses proliferation of hepatoma cells through targeting ten-eleven translocation 1 (TET1) mRNA

    SciTech Connect (OSTI)

    Zhang, Weiying; Lu, Zhanping; Gao, Yuen; Ye, Lihong; Song, Tianqiang; Zhang, Xiaodong

    2015-05-08

    Accumulating evidence indicates that microRNAs are able to act as oncogenes or tumor suppressor genes in human cancer. We previously reported that miR-520b was down-regulated in hepatocellular carcinoma (HCC) and its deregulation was involved in hepatocarcinogenesis. In the present study, we report that miR-520b suppresses cell proliferation in HCC through targeting the ten-eleven translocation 1 (TET1) mRNA. Notably, we identified that miR-520b was able to target 3′-untranslated region (3′UTR) of TET1 mRNA by luciferase reporter gene assays. Then, we revealed that miR-520b was able to reduce the expression of TET1 at the levels of mRNA and protein using reverse transcription-polymerase chain reaction and Western blotting analysis. In terms of function, 5-ethynyl-2-deoxyuridine (EdU) incorporation and colony formation assays demonstrated that the forced miR-520b expression remarkably inhibited proliferation of hepatoma cells, but TET1 overexpression could rescue the inhibition of cell proliferation mediated by miR-520b. Furthermore, anti-miR-520b enhanced proliferation of hepatoma cells, whereas silencing of TET1 abolished anti-miR-520b-induced acceleration of cell proliferation. Then, we validated that the expression levels of miR-520b were negatively related to those of TET1 mRNA in clinical HCC tissues. Thus, we conclude that miR-520b depresses proliferation of liver cancer cells through targeting 3′UTR of TET1 mRNA. Our finding provides new insights into the mechanism of hepatocarcinogenesis. - Highlights: • TET1 is a novel target gene of miR-520b. • TET1 is upregulated in clinical HCC tissues. • MiR-520b is negatively correlated with TET1 in clinical HCC tissues. • MiR-520b depresses the proliferation of HCC cells through targeting TET1 mRNA.

  9. Evaluation of Codisposal Viability for Melt and Dilute DOE-Owned Fuel

    SciTech Connect (OSTI)

    H. Radulescu

    2001-07-31

    There are more than 250 forms of U.S. Department of Energy (DOE)-owned spent nuclear fuel (SNF). Due to the variety of the spent nuclear fuel, the National Spent Nuclear Fuel Program has designated nine representative fuel groups for disposal criticality analyses based on fuel matrix, primary fissile isotope, and enrichment (DOE 2000b, Section 6.6.8). The Melt and Dilute (MD) SNF has been designated as the representative fuel for the high-enriched U-Al fuel group. The MD SNF consists of homogeneous cylindrical ingots with 16.5 in. (419.1 mm) maximum diameter. Two general ingot compositions are considered in the criticality and geochemistry analyses. The first composition consists of 8.2 to 18.2 wt% uranium, enriched at less than 20 wt% U-235 and 0.5 wt% gadolinium, with the balance of the ingot being aluminum. The second composition is identical to the first for uranium and gadolinium, but in this case 2.5 wt% of the ingot is hafnium, with the balance of the ingot being aluminum. The results of the analyses performed will be used to develop waste acceptance criteria. The items that are important to criticality control are identified based on the analysis needs and result sensitivities. Prior to acceptance of fuel from the high-enriched U-Al fuel group for disposal, the important items for the fuel types that are being considered for disposal under the high-enriched U-Al fuel group must be demonstrated to satisfy the conditions determined in this report. The analyses have been performed by following the disposal criticality analysis methodology, which was documented in the ''Disposal Criticality Analysis Methodology Topical Report'' (YMP 2000) submitted to the U.S. Nuclear Regulatory Commission. The methodology includes analyzing the geochemical and physical processes that can breach the waste package and degrade the waste forms and other internal components, as well as the structural, thermal, and shielding analyses, and intact and degraded component criticality

  10. Owning and Driving a Car You Plug In Scott Wilson, Electric Vehicle Association of Greater Washington DC

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Owning and Driving a Car You Plug In Scott Wilson, Electric Vehicle Association of Greater Washington DC US Patent and Trademark Office Electric Vehicle Association of Greater Washington DC www.evadc.org Electric Vehicle Association of Greater Washington DC www.evadc.org EVADC - Owners, Drivers, Builders of Electric Vehicles * Education of the public, promotion of EV's Electric Vehicle Association of Greater Washington DC www.evadc.org 2012 Nissan Leaf 36k miles 2016 Kia Soul EV 7k miles 2014

  11. The RNA-binding protein PCBP2 facilitates gastric carcinoma growth by targeting miR-34a

    SciTech Connect (OSTI)

    Hu, Cheng-En; Liu, Yong-Chao; Zhang, Hui-Dong; Huang, Guang-Jian

    2014-06-13

    Highlights: • PCBP2 is overexpressed in human gastric cancer. • PCBP2 high expression predicts poor survival. • PCBP2 regulates gastric cancer growth in vitro and in vivo. • PCBP2 regulates gastric cancer apoptosis by targeting miR-34a. - Abstract: Gastric carcinoma is the fourth most common cancer worldwide, with a high rate of death and low 5-year survival rate. However, the mechanism underling gastric cancer is still not fully understood. Here in the present study, we identify the RNA-binding protein PCBP2 as an oncogenic protein in human gastric carcinoma. Our results show that PCBP2 is up-regulated in human gastric cancer tissues compared to adjacent normal tissues, and that high level of PCBP2 predicts poor overall and disease-free survival. Knockdown of PCBP2 in gastric cancer cells inhibits cell proliferation and colony formation in vitro, whereas opposing results are obtained when PCBP2 is overexpressed. Our in vivo subcutaneous xenograft results also show that PCBP2 can critically regulate gastric cancer cell growth. In addition, we find that PCBP2-depletion induces apoptosis in gastric cancer cells via up-regulating expression of pro-apoptotic proteins and down-regulating anti-apoptotic proteins. Mechanically, we identify that miR-34a as a target of PCBP2, and that miR-34a is critically essential for the function of PCBP2. In summary, PCBP2 promotes gastric carcinoma development by regulating the level of miR-34a.

  12. Ginsenoside-Rg{sub 1} induces angiogenesis by the inverse regulation of MET tyrosine kinase receptor expression through miR-23a

    SciTech Connect (OSTI)

    Kwok, Hoi-Hin; Chan, Lai-Sheung; Poon, Po-Ying; Yue, Patrick Ying-Kit; Wong, Ricky Ngok-Shun

    2015-09-15

    Therapeutic angiogenesis has been implicated in ischemic diseases and wound healing. Ginsenoside-Rg{sub 1} (Rg{sub 1}), one of the most abundant active components of ginseng, has been demonstrated as an angiogenesis-stimulating compound in different models. There is increasing evidence implicating microRNAs (miRNAs), a group of non-coding RNAs, as important regulators of angiogenesis, but the role of microRNAs in Rg{sub 1}-induced angiogenesis has not been fully explored. In this report, we found that stimulating endothelial cells with Rg{sub 1} could reduce miR-23a expression. In silico experiments predicted hepatocyte growth factor receptor (MET), a well-established mediator of angiogenesis, as the target of miR-23a. Transfection of the miR-23a precursor or inhibitor oligonucleotides validated the inverse relationship of miR-23a and MET expression. Luciferase reporter assays further confirmed the interaction between miR-23a and the MET mRNA 3′-UTR. Intriguingly, ginsenoside-Rg{sub 1} was found to increase MET protein expression in a time-dependent manner. We further demonstrated that ginsenoside-Rg{sub 1}-induced angiogenic activities were indeed mediated through the down-regulation of miR-23a and subsequent up-regulation of MET protein expression, as confirmed by gain- and loss-of-function angiogenic experiments. In summary, our results demonstrated that ginsenoside-Rg{sub 1} could induce angiogenesis by the inverse regulation of MET tyrosine kinase receptor expression through miR-23a. This study has broadened our understanding of the non-genomic effects of ginsenoside-Rg{sub 1,} and provided molecular evidence that warrant further development of natural compound as novel angiogenesis-promoting therapy. - Highlights: • Therapeutic angiogenesis has been implicated in ischemic diseases and wound healing. • Ginsenoside-Rg{sub 1} (Rg{sub 1}) has been demonstrated as an angiogenesis-stimulating compound. • We found that Rg{sub 1} induces angiogenesis by

  13. Analysis of the hydraulic data from the MI fracture zone at the Grimsel Rock Laboratory, Switzerland

    SciTech Connect (OSTI)

    Davey, A.; Karasaki, K.; Long, J.C.S.; Landsfeld, M.; Mensch, A.; Martel, S.J.

    1989-10-01

    One of the major problems in analyzing flow and transport in fractured rock is that the flow may be largely confined to a poorly connected network of fractures. In order to overcome some of this problem, Lawrence Berkeley Laboratory (LBL) has been developing a new type of fracture hydrology model called an equivalent discontinuum model. In this model the authors represent the discontinuous nature of the problem through flow on a partially filled lattice. A key component in constructing an equivalent discontinuum model from this lattice is removing some of the conductive elements such that the system is partially connected in the same manner as the fracture network. This is done through a statistical inverse technique called simulated annealing. The fracture network model is annealed by continually modifying a base model, or template such that the modified systems behave more and more like the observed system. In order to see how the simulated annealing algorithm works, the authors have developed a series of synthetic real cases. In these cases, the real system is completely known so that the results of annealing to steady state data can be evaluated absolutely. The effect of the starting configuration has been studied by varying the percent of conducting elements in the initial configuration. Results have shown that the final configurations converge to about the same percentage of conducting elements. An example using Nagra field data from the Migration Experiment (MI) at Grimsel Rock Laboratory in Switzerland is also analyzed. 24 refs., 33 figs., 3 tabs.

  14. A Flexible Baseline Design for the Advanced High Temperature Reactor Using Metallic Internals (AHTR-MI)

    SciTech Connect (OSTI)

    Peterson, Per F.; Haihua, Zhao

    2006-07-01

    The Advanced High Temperature Reactor (AHTR) is a novel reactor concept that uses clean liquid fluoride salts as a high temperature primary coolant for conventional graphite matrix, coated particle fuels. The very large volumetric heat capacity and excellent natural circulation heat transfer provided by liquid salts enables the design of high-temperature reactors with passive safety features and thermal powers ranging from 2400 to 4000 MW(t). To make optimal use of existing design information, early AHTR designs were modeled upon the S-PRISM sodium pool-reactor design, but introduced an insulated reactor vessel. Subsequent work has shown that liquid-salt cooled systems share important similarities with pressurized water reactors, which use a closed primary loop. This article discusses the use of a closed primary loop immersed in a separate buffer salt tank, which permits the primary loop components to be fabricated from metallic materials (AHTR-MI). This approach may provide several key advantages over open loop approaches, including very large thermal inertia. (authors)

  15. Monocyte to macrophage differentiation-associated (MMD) targeted by miR-140-5p regulates tumor growth in non-small cell lung cancer

    SciTech Connect (OSTI)

    Li, Weina; He, Fei

    2014-07-18

    Highlights: • Expression of MMD is increased in lung cancer tissues. • Knockdown of MMD inhibits growth of A549 and LLC cells in vitro and in vivo. • MMD is a direct functional target of miR-140-5p. • MiR-140-5p/MMD axis regulates Erk1/2 signaling. - Abstract: Monocyte to macrophage differentiation-associated (MMD) is identified in macrophages as a gene associated with the differentiation from monocytes to macrophages. Recent microarray analysis for non-small cell lung cancer (NSCLC) suggests that MMD is an important signature associated with relapse and survival among patients with NSCLC. Therefore, we speculate that MMD likely plays a role in lung cancer. In this study, we found that the protein level of MMD was increased in lung cancer compared to benign lung tissues, and knockdown of MMD inhibited the growth of A549 and Lewis lung cancer cells (LLC) in vitro and in vivo. Integrated analysis demonstrated that MMD was a direct functional target of miR-140-5p. Furthermore, we found that miR-140-5p/MMD axis could affect the cell proliferation of lung cancer cells by regulating Erk signaling. Together, our results highlight the significance of miR-140-5p/MMD axis in lung cancer, and miR-140-5p/MMD axis could serve as new molecular targets for the therapy against lung cancer.

  16. Executive summary of major NuMI lessons learned: a review of relevant meetings of Fermilab's DUSEL Beamline Working Group

    SciTech Connect (OSTI)

    Andrews, Mike; Appel, Jeffrey A.; Bogert, Dixon; Childress, Sam; Cossairt, Don; Griffing, William; Grossman, Nancy; Harding, David; Hylen, Jim; Kuchler, Vic; Laughton, Chris; /Fermilab /Argonne /Brookhaven /LBL, Berkeley

    2009-05-01

    We have gained tremendous experience with the NuMI Project on what was a new level of neutrino beams from a high power proton source. We expect to build on that experience for any new long baseline neutrino beam. In particular, we have learned about some things which have worked well and/or where the experience is fairly directly applicable to the next project (e.g., similar civil construction issues including: tunneling, service buildings, outfitting, and potential claims/legal issues). Some things might be done very differently (e.g., decay pipe, windows, target, beam dump, and precision of power supply control/monitoring). The NuMI experience does lead to identification of critical items for any future such project, and what issues it will be important to address. The DUSEL Beamline Working Group established at Fermilab has been meeting weekly to collect and discuss information from that NuMI experience. This document attempts to assemble much of that information in one place. In this Executive Summary, we group relevant discussion of some of the major issues and lessons learned under seven categories: (1) Differences Between the NuMI Project and Any Next Project; (2) The Process of Starting Up the Project; (3) Decision and Review Processes; (4) ES&H: Environment, Safety, and Health; (5) Local Community Buy-In; (6) Transition from Project Status to Operation; and (7) Some Lessons on Technical Elements. We concentrate here on internal project management issues, including technical areas that require special attention. We cannot ignore, however, two major external management problems that plagued the NuMI project. The first problem was the top-down imposition of an unrealistic combination of scope, cost, and schedule. This situation was partially corrected by a rebaselining. However, the full, desirable scope was never achievable. The second problem was a crippling shortage of resources. Critical early design work could not be done in a timely fashion, leading to

  17. Final Technical Report. Sault Tribe Building Efficiency Audits of Tribally-Owned Governmental Buildings and Residential Tribal Housing

    SciTech Connect (OSTI)

    Holt, Jeffrey W.

    2015-03-27

    The Tribe is working to reduce energy consumption and expense in Tribally-owned governmental buildings and low income housing sites. In 2009, the Tribe applied to the U. S. Department of Energy for funding to conduct energy audits of Tribally-owned governmental buildings. Findings from the energy audits would define the extent and types of energy efficiency improvements needed, establish a basis for energy priorities, strategies and action plans, and provide a benchmark for measuring improvements from energy efficiency implementations. In 2010, the DOE awarded a grant in the amount of $95,238 to the Tribe to fund the energy audits of nine governmental buildings and to pay for travel expenses associated with attendance and participation at the DOE annual program reviews. In 2011, the Tribe applied for and was awarded a DOE grant in the amount of $75,509 to conduct energy audits of the remaining 30 Tribally-owned governmental buildings. Repeating mobilization steps performed during the first DOE energy audits grant, the Tribe initiated the second round of governmental building energy audits by completing energy auditor procurement. The selected energy auditor successfully passed DOE debarment and Sault Tribe background clearances. The energy audits contract was awarded to U. P. Engineers and Architects, Inc. of Sault Ste. Marie, Michigan. The Tribe continued mobilizing for the energy audits by providing the energy auditor with one year of electric, gas and water utility invoice copies per building, as well as supplemental building information, such as operating hours. The Tribe also contacted building occupants to coordinate scheduling for the on-site energy audit inspections and arranged for facilities management personnel to guide the energy auditor through the buildings and answer questions regarding building systems.

  18. Hepatitis B virus X protein mutant HBxΔ127 promotes proliferation of hepatoma cells through up-regulating miR-215 targeting PTPRT

    SciTech Connect (OSTI)

    Liu, Fabao; You, Xiaona; Chi, Xiumei; Wang, Tao; Ye, Lihong; Niu, Junqi; Zhang, Xiaodong

    2014-02-07

    Highlights: • Relative to wild type HBx, HBX mutant HBxΔ127 strongly enhances cell proliferation. • Relative to wild type HBx, HBxΔ127 remarkably up-regulates miR-215 in hepatoma cells. • HBxΔ127-elevated miR-215 promotes cell proliferation via targeting PTPRT mRNA. - Abstract: The mutant of virus is a frequent event. Hepatitis B virus X protein (HBx) plays a vital role in the development of hepatocellular carcinoma (HCC). Therefore, the identification of potent mutant of HBx in hepatocarcinogenesis is significant. Previously, we identified a natural mutant of the HBx gene (termed HBxΔ127). Relative to wild type HBx, HBxΔ127 strongly enhanced cell proliferation and migration in HCC. In this study, we aim to explore the mechanism of HBxΔ127 in promotion of proliferation of hepatoma cells. Our data showed that both wild type HBx and HBxΔ127 could increase the expression of miR-215 in hepatoma HepG2 and H7402 cells. However, HBxΔ127 was able to significantly increase miR-215 expression relative to wild type HBx in the cells. We identified that protein tyrosine phosphatase, receptor type T (PTPRT) was one of the target genes of miR-215 through targeting 3′UTR of PTPRT mRNA. In function, miR-215 was able to promote the proliferation of hepatoma cells. Meanwhile anti-miR-215 could partially abolish the enhancement of cell proliferation mediated by HBxΔ127 in vitro. Knockdown of PTPRT by siRNA could distinctly suppress the decrease of cell proliferation mediated by anti-miR-215 in HepG2-XΔ127/H7402-XΔ127 cells. Moreover, we found that anti-miR-215 remarkably inhibited the tumor growth of hepatoma cells in nude mice. Collectively, relative to wild type HBx, HBxΔ127 strongly enhances proliferation of hepatoma cells through up-regulating miR-215 targeting PTPRT. Our finding provides new insights into the mechanism of HBx mutant HBxΔ127 in promotion of proliferation of hepatoma cells.

  19. Mitsubishi iMiEV: An Electric Mini-Car in NREL's Advanced Technology Vehicle Fleet (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2011-10-01

    This fact sheet highlights the Mitsubishi iMiEV, an electric mini-car in the advanced technology vehicle fleet at the National Renewable Energy Laboratory (NREL). In support of the U.S. Department of Energy's fast-charging research efforts, NREL engineers are conducting charge and discharge performance testing on the vehicle. NREL's advanced technology vehicle fleet features promising technologies to increase efficiency and reduce emissions without sacrificing safety or comfort. The fleet serves as a technology showcase, helping visitors learn about innovative vehicles that are available today or are in development. Vehicles in the fleet are representative of current, advanced, prototype, and emerging technologies.

  20. Who owns the recyclables

    SciTech Connect (OSTI)

    Parker, B.

    1994-05-01

    On March 31, the California Supreme Court decided the much awaited Rancho Mirage'' case (Waste Management of the Desert, Inc., and the City of Rancho Mirage v. Palm Springs Recycling Center, Inc.), and held that the California Integrated Waste Management Act of 1989 does not allow an exclusive franchise for the collection of recyclables not discarded by their owner.'' This ends a three-year slugfest between secondary materials processors in the state and municipalities and their franchised garbage haulers who also collect and process recyclables as part of their exclusive arrangement. Central to this nationally-watched litigation is a most fundamental question in waste management: at what point in time do articles in the solid waste stream become actual or potentially valuable secondary materials

  1. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Investor-Owned Utility Savings Category: Geothermal Electric, Solar Thermal Process Heat, Solar Photovoltaics, Wind (All), Biomass, Hydroelectric, Geothermal Heat Pumps,...

  2. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Investor-Owned Utility, Municipal Utilities, Cooperative Utilities Savings Category: Solar Water Heat, Geothermal Electric, Solar Thermal Electric, Solar Thermal Process Heat,...

  3. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    (ERCOT), the program administrator for the Texas Renewable Energy Credit Trading Program, Texas sur... Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Solar...

  4. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Public Benefits Funds for Renewables and Efficiency California's 1996 electric industry restructuring legislation (AB 1890) directed the state's three major investor-owned...

  5. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    in the District. In October 2008 the RPS was amended by... Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Solar Water Heat, Solar Space Heat, Geothermal...

  6. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Texas Renewable Energy Credit Trading Program, Texas sur... Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Solar Water Heat, Geothermal Electric, Solar...

  7. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    electric load by 2025 and thereafter. Of this percentage,... Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Solar Water Heat, Solar Space Heat, Geothermal...

  8. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    gas and electric utilities, failure to submit an energ... Eligibility: Investor-Owned Utility, Municipal Utilities, Cooperative Utilities, Retail Supplier Savings Category:...

  9. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Solar Water Heat, Solar Space Heat, Geothermal Electric,...

  10. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Solar Water Heat, Solar Space Heat, Geothermal Electric, Solar Thermal Electric, Solar Thermal...

  11. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Energy Efficiency Standard for Focus on Energy Origin Eligibility: Investor-Owned Utility, Municipal Utilities, Cooperative Utilities Savings Category: Solar Water Heat, Geothermal...

  12. Net Metering

    Office of Energy Efficiency and Renewable Energy (EERE)

    Kansas adopted the Net Metering and Easy Connection Act in May 2009, which established net metering for customers of investor-owned utilities (IOUs). 

  13. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    legislation concerning spent nuclear fuel at Xcel Energy's Prairie Island Nuclear Plant. The... Eligibility: Commercial, Industrial, Investor-Owned Utility, Local...

  14. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    a renewable portfolio standard (RPS) without having restructured its electric utility industry. The RPS sets a total goal... Eligibility: Investor-Owned Utility, Municipal...

  15. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Wave Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Net Metering New Jersey's net-metering rules require state's investor-owned utilities and energy suppliers...

  16. Property:EIA/861/Ownership | Open Energy Information

    Open Energy Information (Open El) [EERE & EIA]

    10 different types of ownership categories: Federal; Political Subdivision; Municipal Marketing Authority; Cooperative; State; Municipal; Investor-Owned; Retail Power Marketer...

  17. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Energy Efficiency Resource Standard In 2007, the Minnesota legislature passed the Next Generation Energy Act (NGEA), which requires both electric and natural gas investor-owned...

  18. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    demand-side management (DSM) electric savings goals leading to 2.0% reduction of electricity sa... Eligibility: Investor-Owned Utility, Retail Supplier Savings Category:...

  19. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    EE, Wind (Small), Hydroelectric (Small), Anaerobic Digestion, Fuel Cells using Renewable Fuels Alternative Energy Portfolio Standard Background Eligibility: Investor-Owned...

  20. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Appeals upheld the constitutionality Colorado's renewable energy standard (Energy & Environment Legal, et al v. Epel, et al, case number 14-1216). Eligibility: Investor-Owned...

  1. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    sur... Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Solar Water Heat, Geothermal Electric, Solar Thermal Electric, Solar Photovoltaics, Wind (All),...

  2. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Wind (All), Biomass, Hydroelectric, Tidal, Wave, Ocean Thermal, Solar Pool Heating, Wind (Small) Renewables Portfolio Goal Goals... Eligibility: Investor-Owned Utility,...

  3. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Tidal Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Net Metering New Jersey's net-metering rules require state's investor-owned utilities and energy suppliers...

  4. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Geothermal Heat Pumps, Landfill Gas, Tidal, Wave, Ocean Thermal, Wind (Small) Renewables Portfolio Standard Eligible Technologies: Eligibility: Investor-Owned Utility, Local...

  5. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Ocean Thermal, Wind (Small), Anaerobic Digestion, Fuel Cells using Renewable Fuels Renewables Portfolio Standard Eligible Technologies: Eligibility: Investor-Owned Utility, Local...

  6. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    technologies Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Solar Water Heat, Solar Space Heat, Geothermal Electric, Solar Thermal Electric, Solar Thermal...

  7. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    sa... Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Combined Heat & Power, Dishwasher, RefrigeratorsFreezers, Lighting, Lighting ControlsSensors,...

  8. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    utilities (IOUs) to engage in demand response and adopt demand-side management (DSM)... Eligibility: Investor-Owned Utility Savings Category: CustomOthers pending...

  9. Table 3. Top five retailers of electricity, with end use sectors...

    U.S. Energy Information Administration (EIA) (indexed site)

    Florida" "megawatthours" ,"Entity","Type of provider","All sectors","Residential","Commercial","Industrial","Transportation" 1,"Florida Power & Light Co","Investor-owned",104431096...

  10. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    fees as part of the Energy Efficiency Portfolio Standard (EEPS). For both natural gas and electric utilities, failure to submit an energ... Eligibility: Investor-Owned...

  11. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Agricultural Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Net Metering New Jersey's net-metering rules require state's investor-owned utilities and energy...

  12. Interconnection Standards

    Office of Energy Efficiency and Renewable Energy (EERE)

    Massachusetts' interconnection standards apply to all forms of distributed generation (DG), including renewables, and to all customers of the state's three investor-owned utilities (Unitil,...

  13. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Photovoltaics Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Net Metering New Jersey's net-metering rules require state's investor-owned utilities and energy...

  14. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Reliability Council of Texas (ERCOT), the program administrator for the Texas Renewable Energy Credit Trading Program, Texas sur... Eligibility: Investor-Owned Utility, Retail...

  15. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Thermal Electric Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility,...

  16. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    DuctAir sealing Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility,...

  17. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Wind (Small), Hydroelectric (Small), Geothermal Direct-Use Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail Supplier...

  18. Table 3. Top five retailers of electricity, with end use sectors...

    U.S. Energy Information Administration (EIA) (indexed site)

    Washington" "megawatthours" ,"Entity","Type of provider","All sectors","Residential","Commercial","Industrial","Transportation" 1,"Puget Sound Energy Inc","Investor-owned",20568948...

  19. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    districts; community colleges; and universities that receive electricity and natural gas distribution service from... Eligibility: Investor-Owned Utility, Local Government,...

  20. Renewables Portfolio Standard | Department of Energy

    Energy.gov (indexed) [DOE]

    Investor-Owned Utility Local Government Savings Category Geothermal Electric Solar Thermal Electric Solar Photovoltaics Wind (All) Biomass Municipal Solid Waste Landfill Gas Tidal...

  1. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Geothermal Electric Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Net Metering New Jersey's net-metering rules require state's investor-owned utilities and...

  2. Municipal Consortium LED Street Lighting Workshop Presentations...

    Energy Savers

    Massaro, Glenn Cooper, Matthew Mayrl, City of Boston System Reliability for Outdoor SSL Tom Bevan, CreeBetaLED Investor Owned Utility Financial Perspective Michael Stevens, ...

  3. Idaho/Transmission | Open Energy Information

    Open Energy Information (Open El) [EERE & EIA]

    for the grid that links all of these service territories. Idaho Power, Bonneville Power Administration, Rocky Mountain Power, and Avista are the investor owned utilities in...

  4. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Portfolio Standard and creating two new classes of renewable energy credits (RECs): Zero Emission Renewable Energy... Eligibility: Commercial, Industrial, Investor-Owned...

  5. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Standard and creating two new classes of renewable energy credits (RECs): Zero Emission Renewable Energy... Eligibility: Commercial, Construction, Industrial, Investor-Owned...

  6. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    for individuals, businesses, and local governments that generate electricity from solar power, wind power or... Eligibility: Commercial, Investor-Owned Utility, Local...

  7. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Yes; specific technologies not identified Energy Efficiency Portfolio Standard Origin Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Combined Heat &...

  8. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Utility Savings Category: CustomOthers pending approval Energy Reduction Goals Origin Eligibility: Investor-Owned Utility, Local Government Savings Category: CustomOthers...

  9. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Others pending approval, Other EE Energy Efficiency Standard for Focus on Energy Origin Eligibility: Investor-Owned Utility, Municipal Utilities, Cooperative Utilities...

  10. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Windows, CustomOthers pending approval, Other EE Energy Efficiency Standard Origin Eligibility: Investor-Owned Utility, Local Government, Municipal Utilities,...

  11. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    LED Lighting, Commercial Refrigeration Equipment Energy Efficiency Portfolio Standard Origin Eligibility: Investor-Owned Utility Savings Category: Solar Water Heat, Combined Heat...

  12. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Fuels, Other Distributed Generation Technologies Energy Efficiency Portfolio Standard Origin Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Combined Heat &...

  13. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Standard Requirements Eligibility: Investor-Owned Utility Savings Category: Solar Water Heat, Solar Space Heat, Geothermal Electric, Solar Thermal Electric, Solar Thermal...

  14. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Utility Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  15. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Supplier Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  16. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Pumps Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  17. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Washers Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  18. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    VFDs Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  19. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    sealing Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  20. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Freezers Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  1. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Islands- Renewables Portfolio Targets Photovoltaic Energy, wind energy, hydroelectric energy, landfill gas, biomass, ocean and microturbine systems. Eligibility: Investor-Owned...

  2. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Biomass Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Net Metering New Jersey's net-metering rules require state's investor-owned utilities and energy suppliers...

  3. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Eligibility: Investor-Owned Utility, Municipal Utilities, Cooperative Utilities, Retail Supplier Savings Category: Solar Water Heat, Solar Space Heat, Geothermal Electric,...

  4. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    gas distribution service from... Eligibility: Investor-Owned Utility, Local Government, State Government, Federal Government Savings Category: Geothermal Heat Pumps, Refrigerators...

  5. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    public school districts; community colleges; and universities that receive electricity and natural gas distribution service from... Eligibility: Investor-Owned Utility,...

  6. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Eligibility: Investor-Owned Utility Savings Category: Solar Water Heat, Solar Space Heat, Geothermal Electric, Solar Thermal Electric, Solar Thermal Process Heat, Solar...

  7. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Investor-Owned Utility, Retail Supplier Savings Category: Solar Water Heat, Solar Space Heat, Geothermal Electric, Solar Thermal Electric, Solar Thermal Process Heat, Solar...

  8. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Yes; specific technologies not identified Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Energy Efficiency Resource Standard Origin Eligibility: Investor-Owned...

  9. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Motor VFDs Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  10. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Tribal Government Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Net Metering New Jersey's net-metering rules require state's investor-owned utilities and energy...

  11. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Federal Government Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Net Metering New Jersey's net-metering rules require state's investor-owned utilities and energy...

  12. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Local Government Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Net Metering New Jersey's net-metering rules require state's investor-owned utilities and energy...

  13. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    State Government Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Net Metering New Jersey's net-metering rules require state's investor-owned utilities and energy...

  14. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Federal Government Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Net Metering New Jersey's net-metering rules require state's investor-owned utilities and...

  15. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Local Government Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Renewables Portfolio Standard Eligible Technologies: Eligibility: Investor-Owned Utility, Local...

  16. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Investor-Owned Utility, Local Government Savings Category: Solar Water Heat, Solar Photovoltaics, Wind (All), Biomass, Hydroelectric, Combined Heat & Power, Fuel Cells...

  17. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Advanced Energy Fund History Eligibility: Commercial, Industrial, Investor-Owned Utility, Municipal Utilities,...

  18. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    not identified, Anaerobic Digestion, Fuel Cells using Renewable Fuels Advanced Energy Fund History Eligibility: Commercial, Industrial, Investor-Owned Utility, Municipal...

  19. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Yes; specific technologies not identified, Wind (Small), Hydroelectric (Small) Advanced Energy Fund History Eligibility: Commercial, Industrial, Investor-Owned Utility, Municipal...

  20. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Eligibility: Investor-Owned Utility, Local Government Savings Category: Solar Water Heat, Solar Photovoltaics, Wind (All), Biomass, Hydroelectric, Combined Heat & Power, Fuel Cells...

  1. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Other EE, Wind (Small), Hydroelectric (Small), Geothermal Direct-Use Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  2. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Motors, CustomOthers pending approval, Other EE, Wind (Small) Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  3. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    EE, Wind (Small), Personal Computing Equipment, Tankless Water Heater Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  4. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Wind (Small), Hydroelectric (Small), Fuel Cells using Renewable Fuels Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  5. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Equipment, Other EE, Wind (Small), Fuel Cells using Renewable Fuels Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  6. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Energy Portfolio Standard The following schedule is currently in effect: Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Solar Water Heat, Solar Space Heat,...

  7. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Service Commission (PSC) to establish a statewide net metering program for renewable-energy systems within... Eligibility: Commercial, Industrial, Investor-Owned Utility, Local...

  8. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Puerto Rico's adaptation of renewable energy and to reduce the island's reliance on fossil fuels. This is especially... Eligibility: Commercial, Industrial, Investor-Owned...

  9. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    legislation (AB 1890) directed the state's three major investor-owned utilities (Southern California Edison, Pacific Gas and Electric Company, and... Eligibility: Commercial,...

  10. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    a new Clean Energy Standard mandating 50% of the electricity consumed in NY to come from clean... Eligibility: Investor-Owned Utility, Local Government Savings Category: Solar...

  11. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Clean Energy Portfolio Goal Eligible Technologies Eligibility: Investor-Owned Utility, Municipal Utilities, Cooperative Utilities, Retail Supplier Savings Category: Solar Water...

  12. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    using Renewable Fuels Energy Portfolio Standard The following schedule is currently in effect: Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Solar Water...

  13. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    require state's investor-owned utilities and energy suppliers (and certain competitive municipal utilities and electric cooperatives) to offer net metering at non-......

  14. Market Segmentation and Energy Efficiency Program Design

    Energy.gov [DOE]

    Describe the existing state of market segmentation among California’s electric utilities, with an emphasis on the investor-owned utilities. Author: Steve J. Moss

  15. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Heat Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  16. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Ceiling Fan Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  17. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    The remaining funds support low-income housing energy... Eligibility: Commercial, Industrial, Investor-Owned Utility, Local Government, Nonprofit, Municipal Utilities,...

  18. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    and creating two new classes of renewable energy credits (RECs): Zero Emission Renewable Energy... Eligibility: Commercial, Industrial, Investor-Owned Utility, Nonprofit,...

  19. SGIP Smart Grid Interoperabilty Panel

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    SGIP Mission SGIP Members Electric Utilities Investor Owned Utilities, Rural ... - 08 Standard DR & DER Signals - 09 Map IEEE 1815 to IEC 61850 - 12 Wind Plant ...

  20. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    IOUs are required to offer customers a voluntary program for purchasing renewable energy. The volunt... Eligibility: Investor-Owned Utility, Municipal Utilities, Cooperative...

  1. Interconnection Guidelines

    Energy.gov [DOE]

    The Missouri Public Service Commission (PSC) adopted administrative rules for investor-owned utilities that included simplified interconnection standards, and electric cooperatives and municipal ...

  2. Interconnection Guidelines

    Energy.gov [DOE]

    Rocky Mountain Power (PacifiCorp) has developed an interconnection application for net metering. All other electric utilities in Wyoming -- investor-owned utilities and rural electric associations...

  3. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Metering New Jersey's net-metering rules require state's investor-owned utilities and energy suppliers (and certain competitive municipal utilities and electric cooperatives) to...

  4. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Windows Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  5. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    dedicated crops grown for energy production and organic waste biomass, hydropower th... Eligibility: Commercial, Industrial, Investor-Owned Utility, Municipal Utilities,...

  6. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    New Jersey's net-metering rules require state's investor-owned utilities and energy suppliers (and certain competitive municipal utilities and electric cooperatives) to offer...

  7. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    upheld the constitutionality Colorado's renewable energy standard (Energy & Environment Legal, et al v. Epel, et al, case number 14-1216). Eligibility: Investor-Owned Utility,...

  8. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Portfolio Standard Requirements Eligibility: Investor-Owned Utility Savings Category: Solar Water Heat, Solar Space Heat, Geothermal Electric, Solar Thermal Electric, Solar...

  9. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    to meet all the scoring criteria. In particular reimburs... Eligibility: Commercial, Construction, Industrial, Investor-Owned Utility, Local Government, Municipal Utilities,...

  10. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Chillers Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  11. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Heat Pumps Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  12. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Dishwasher Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  13. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    ControlsSensors Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned...

  14. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Air conditioners Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility,...

  15. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Lighting Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  16. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Service Department (PSC) to establish a new Clean Energy Standard mandating 50% of the electricity consumed in NY to come from clean... Eligibility: Investor-Owned Utility,...

  17. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    -- were required to contribute revenue generated from a surcharge on customers' electricity use. In 1997, the... Eligibility: Commercial, Industrial, Investor-Owned...

  18. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    governments; public school districts; community colleges; and universities that receive electricity and natural gas distribution service from... Eligibility: Investor-Owned...

  19. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Solar Water Heat, Solar Space Heat, Geothermal Electric, Solar Thermal Electric, Solar Thermal Process...

  20. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Industrial Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Net Metering New Jersey's net-metering rules require state's investor-owned utilities and energy...

  1. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    in effect: Eligibility: Investor-Owned Utility, Retail Supplier Savings Category: Solar Water Heat, Solar Space Heat, Geothermal Electric, Solar Thermal Electric, Solar...

  2. Business Energy Efficiency Rebate for Existing Buildings | Department...

    Energy.gov (indexed) [DOE]

    institutional customers of any of the state's investor owned utilities to increase the energy efficiency of their existing buildings. The standard incentive program provides...

  3. Net Metering

    Energy.gov [DOE]

    Missouri enacted legislation in June 2007 requiring all electric utilities—investor-owned utilities, municipal utilities, and electric cooperatives—to offer net metering to customers with systems...

  4. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    businesses, and local governments that generate electricity from solar power, wind power or... Eligibility: Commercial, Investor-Owned Utility, Local Government, Municipal...

  5. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    in renewable energy facilities between 1 MW and 10 MW with a cumulative installed capacity equal to one p... Eligibility: Investor-Owned Utility, Municipal Utilities,...

  6. Renewable Energy Standard

    Energy.gov [DOE]

    In October 2008, Michigan enacted the Clean, Renewable, and Efficient Energy Act (Public Act 295), requiring the state's investor-owned utilities, alternative retail suppliers, electric...

  7. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    New Jersey's net-metering rules require state's investor-owned utilities and energy suppliers (and certain competitive municipal utilities and electric cooperatives) to offer net...

  8. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Standard and creating two new classes of renewable energy credits (RECs): Zero Emission Renewable Energy... Eligibility: Commercial, Industrial, Investor-Owned Utility,...

  9. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Solar Photovoltaics, Wind (All), Wind (Small) Net Metering New Jersey's net-metering rules require state's investor-owned utilities and energy suppliers (and certain...

  10. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Rebates & Savings Tax Credits, Rebates & Savings Net Metering New Jersey's net-metering rules require state's investor-owned utilities and energy suppliers (and certain...

  11. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Fuels Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  12. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    USDA- Biorefinery Assistance Program The project must meet the following requirements: Eligibility: Commercial, Construction, Industrial, Investor-Owned Utility, Local Government,...

  13. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    for Renewables and Efficiency California's 1996 electric industry restructuring legislation (AB 1890) directed the state's three major investor-owned utilities (Southern...

  14. Public Benefits Funds for Renewables and Efficiency

    Energy.gov [DOE]

    California's 1996 electric industry restructuring legislation (AB 1890) directed the state’s three major investor-owned utilities (Southern California Edison, Pacific Gas and Electric Company, and...

  15. Renewable Energy Standard

    Office of Energy Efficiency and Renewable Energy (EERE)

    In 2007, Minnesota legislation modified the state's 2001 voluntary renewable energy objective to create a mandatory renewable portfolio standard (RPS). Public utilities (i.e., investor-owned...

  16. Minwind Energy LLC | Open Energy Information

    Open Energy Information (Open El) [EERE & EIA]

    Energy LLC Place: Luverne, Minnesota Zip: 56156 Sector: Wind energy Product: Wind energy business that allows local investors (at least 85% farmers) to own commercial scale...

  17. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    or regulations that place unreasonable limits on or hinder the performance of wind energy... Eligibility: Commercial, Industrial, Investor-Owned Utility, Local Government,...

  18. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Sensors Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility, Retail...

  19. Entity State Ownership Residential Commercial Industrial Transportation

    U.S. Energy Information Administration (EIA) (indexed site)

    Revenue for Delivery Service Providers (Data from form EIA-861 schedule 4C) Entity State Ownership Residential Commercial Industrial Transportation Total Pacific Gas & Electric Co CA Investor Owned 174,506 435,933 237,898 4,192 852,529 San Diego Gas & Electric Co CA Investor Owned 693 100,429 128,369 0 229,490 Southern California Edison Co CA Investor Owned 4,219 532,646 85,532 0 622,397 Connecticut Light & Power Co CT Investor Owned 338,794 492,975 101,437 5,687 938,894 United

  20. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Anaerobic Digestion Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Net Metering New Jersey's net-metering rules require state's investor-owned utilities and...

  1. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Municipal Solid Waste Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Renewables Portfolio Standard Eligible Technologies: Eligibility: Investor-Owned Utility,...

  2. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    rules require state's investor-owned utilities and energy suppliers (and certain competitive municipal utilities and electric cooperatives) to offer net metering at non-......

  3. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    utilities (Alliant, Minnesota Power, Otter Tail Power Company, Xcel Energy) in the Community Solar Gardens... Eligibility: Commercial, Industrial, Investor-Owned Utility, Local...

  4. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Clothes Washers Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned Utility,...

  5. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Council of Texas (ERCOT), the program administrator for the Texas Renewable Energy Credit Trading Program, Texas sur... Eligibility: Investor-Owned Utility, Retail Supplier Savings...

  6. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    competitive electricity providers must refer to the disclosure label in all written marketing materials promoting available generation service. Eligibility: Investor-Owned...

  7. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Lighting ControlsSensors Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned...

  8. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    not identified, Wind (Small), Fuel Cells using Renewable Fuels Advanced Energy Fund History Eligibility: Commercial, Industrial, Investor-Owned Utility, Municipal Utilities,...

  9. Energy Optimization Standard

    Energy.gov [DOE]

    In October 2008, Michigan enacted the Clean, Renewable, and Efficient Energy Act, Public Act 295, requiring the state's investor-owned utilities, alternative retail suppliers, electric cooperatives...

  10. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    on the gross receipts from the sale, lease, or rental of personal property for use in a community-based energy dev... Eligibility: Commercial, Construction, Investor-Owned...

  11. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    by the Electric Reliability Council of Texas (ERCOT), the program administrator for the Texas Renewable Energy Credit Trading Program, Texas sur... Eligibility: Investor-Owned...

  12. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Council of Texas (ERCOT), the program administrator for the Texas Renewable Energy Credit Trading Program, Texas sur... Eligibility: Investor-Owned Utility, Retail...

  13. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Thermal, Fuel Cells using Renewable Fuels, Other Distributed Generation Technologies Renewables Portfolio Standard Eligible Technologies: Eligibility: Investor-Owned Utility, Local...

  14. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    energy, photovoltaics, dedicated crops grown for energy production and organic waste biomass, hydropower th... Eligibility: Commercial, Industrial, Investor-Owned Utility,...

  15. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Fuel Cells using Renewable Fuels Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Renewables Portfolio Standard Eligible Technologies: Eligibility: Investor-Owned...

  16. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    to establish demand-side management (DSM) electric savings goals leading to 2.0% reduction of electricity sa... Eligibility: Investor-Owned Utility, Retail Supplier Savings...

  17. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    efficiency goals for the State. Previous state mandated energy efficiency program- EmPOWER Maryland Act is... Eligibility: Investor-Owned Utility, Municipal Utilities, Retail...

  18. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Fuel Cells using Renewable Fuels Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Net Metering New Jersey's net-metering rules require state's investor-owned...

  19. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    RefrigeratorsFreezers Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Alternative Energy Portfolio Standard Eligible technologies Eligibility: Investor-Owned...

  20. Table 3. Top five retailers of electricity, with end use sectors...

    U.S. Energy Information Administration (EIA) (indexed site)

    4,"Duke Energy Progress - (NC)","Investor-owned",6559067,2292609,1804594,2461864,0 5,"Berkeley Electric Coop Inc","Cooperative",2095651,1269704,244465,581482,0 " ","Total ...

  1. Fuel Mix Disclosure | Department of Energy

    Energy.gov (indexed) [DOE]

    Florida Program Type Generation Disclosure Summary In March 1999, the Florida Public Service Commission issued an order requiring the state's investor-owned electric utilities,...

  2. Market Segmentation and Energy Efficiency Program Design (2008 CIEE Report)

    Office of Energy Efficiency and Renewable Energy (EERE)

    This paper describes the existing state of market segmentation among California’s electric utilities, with an emphasis on the investor-owned utilities (IOUs).

  3. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    companies and electric service suppliers also must provide this information in most marketing materials. Eligibility: Investor-Owned Utility, Municipal Utilities, Cooperative...

  4. Table 3. Top five retailers of electricity, with end use sectors...

    U.S. Energy Information Administration (EIA) (indexed site)

    Iowa" "megawatthours" ,"Entity","Type of provider","All sectors","Residential","Commercial","Industrial","Transportation" 1,"MidAmerican Energy Co","Investor-owned",20585461,570529...

  5. Table 3. Top five retailers of electricity, with end use sectors...

    U.S. Energy Information Administration (EIA) (indexed site)

    Montana" "megawatthours" ,"Entity","Type of provider","All sectors","Residential","Commercial","Industrial","Transportation" 1,"NorthWestern Energy LLC - (MT)","Investor-owned",597...

  6. Table 3. Top five retailers of electricity, with end use sectors...

    U.S. Energy Information Administration (EIA) (indexed site)

    Kansas" "megawatthours" ,"Entity","Type of provider","All sectors","Residential","Commercial","Industrial","Transportation" 1,"Westar Energy Inc","Investor-owned",9973395,3434301,4...

  7. Table 3. Top five retailers of electricity, with end use sectors...

    U.S. Energy Information Administration (EIA) (indexed site)

    Carolina" "megawatthours" ,"Entity","Type of provider","All sectors","Residential","Commercial","Industrial","Transportation" 1,"Duke Energy Carolinas, LLC","Investor-owned",567506...

  8. Table 3. Top five retailers of electricity, with end use sectors...

    U.S. Energy Information Administration (EIA) (indexed site)

    Ohio" "megawatthours" ,"Entity","Type of provider","All sectors","Residential","Commercial","Industrial","Transportation" 1,"First Energy Solutions Corp.","Investor-owned",41994756...

  9. Table 3. Top five retailers of electricity, with end use sectors...

    U.S. Energy Information Administration (EIA) (indexed site)

    Pennsylvania" "megawatthours" ,"Entity","Type of provider","All sectors","Residential","Commercial","Industrial","Transportation" 1,"First Energy Solutions Corp.","Investor-owned",...

  10. Table 3. Top five retailers of electricity, with end use sectors...

    U.S. Energy Information Administration (EIA) (indexed site)

    Indiana" "megawatthours" ,"Entity","Type of provider","All sectors","Residential","Commercial","Industrial","Transportation" 1,"Duke Energy Indiana Inc","Investor-owned",28224148,9...

  11. Table 3. Top five retailers of electricity, with end use sectors...

    U.S. Energy Information Administration (EIA) (indexed site)

    Texas" "megawatthours" ,"Entity","Type of provider","All sectors","Residential","Commercial","Industrial","Transportation" 1,"Reliant Energy Retail Services","Investor-owned",38670...

  12. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    or "useful thermal energy" generated by any of the following resources, provided the generator began operation after January 1, 20... Eligibility: Investor-Owned Utility, Retail...

  13. NMAC 17.9.569 Interconnection of Generating Facilities with a...

    Open Energy Information (Open El) [EERE & EIA]

    Greater than 10 MWLegal Abstract This rule applies to every electric utility including rural electric cooperatives and investor-owned utilities within the state of New Mexico...

  14. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Net Metering Kansas adopted the Net Metering and Easy Connection Act in May 2009, which established net metering for customers of investor-owned utilities (IOUs). Eligibility:...

  15. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    mills per kWh) and applied only to... Eligibility: Commercial, Industrial, Investor-Owned Utility, Municipal Utilities, Residential, Cooperative Utilities, Institutional Savings...

  16. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Electric Reliability Council of Texas (ERCOT), the program administrator for the Texas Renewable Energy Credit Trading Program, Texas sur... Eligibility: Investor-Owned Utility,...

  17. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    must ensure that a percentage of the electricity sold to retail customers in-state be derived from newer eligible ren... Eligibility: Investor-Owned Utility, Local Government,...

  18. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov (indexed) [DOE]

    Resource Standard In 2007, the Minnesota legislature passed the Next Generation Energy Act (NGEA), which requires both electric and natural gas investor-owned utilities to reduce...

  19. Tax Credits, Rebates & Savings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Municipal Utilities Tax Credits, Rebates & Savings Tax Credits, Rebates & Savings Renewable Portfolio Standard Eligible Technologies Eligibility: Investor-Owned Utility, Local...

  20. Table 3. Top five retailers of electricity, with end use sectors...

    U.S. Energy Information Administration (EIA) (indexed site)

    Dakota" "megawatthours" ,"Entity","Type of provider","All sectors","Residential","Commercial","Industrial","Transportation" 1,"Northern States Power Co - Minnesota","Investor-owned...