National Library of Energy BETA

Sample records for kit qiagen valencia

  1. Comparison of Packed Beds and Qiagen Columns for Recovering Trace Amounts of B. anthracis DNA from Liquid Suspensions

    SciTech Connect

    Sorensen, K; Arroyo, E; Erler, A; Christian, A T; Camp, D; Wheeler, E K

    2006-06-23

    The goal of this work was to optimize and evaluate LLNL's in-bed amplification technology to improve the level of detection for suspensions containing trace amounts of anthracis DNA. The binding/cleaning performance of the packed bed is compared to the conventional commercial approach; Qiagen column cleanup and elution, followed by detection through an ex-situ amplification process. Five liquid suspensions were spiked with B.anthracis DNA in concentration series. These suspensions were: (1) water, (2) water with EDTA, (3) dirty water from carpet extraction, (4) dirty carpet extraction with phosphate buffered saline (PBS) plus 0.1% Tween 20 plus 0.1% gelatin, and (5) a subway aerosol collected in water. Each suspension matrix was spiked with DNA and injected (in replicate) into either Qiagen Microcolumns (using the kit processing instructions) or LLNL's packed bed (using the LLNL in-bed purification and amplification protocol). The process output was assayed by quantitative polymerase chain reaction (QPCR). Table ES-1 shows the level of DNA (pg per 100 uL of input suspension) that resulted in successful amplification for all reactions (X=Y), and the level for which at least one of the reactions was successful (X>0). For each suspension and DNA concentration, there were Y QPCR assays of which X showed successful amplification. LLNL's packed bed technology outperformed Qiagen Microcolumns for all five suspensions, typically by one order of magnitude in both the limit of assured detection (all reactions positive), and the lower limit of detection (some reactions positive).

  2. CEEI Valencia | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Valencia Jump to: navigation, search Name: CEEI Valencia Place: Spain Sector: Services Product: General Financial & Legal Services ( Charity Non-profit Association )...

  3. Press Kit

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    kit Press Kit These fact sheets discuss various National Ignition Facility-related topics in a printer-friendly PDF format: What is NIF What is NIF? The National Ignition Facility (NIF) uses the world's largest laser to compress and heat BB-sized capsules of fusion fuel with the goal of thermonuclear ignition. NIF experiments produce temperatures and densities similar to those in stars and nuclear weapons. Download the PDF NIF at a Glance NIF at a Glance Experiments conducted on NIF are making

  4. Media Kit | Jefferson Lab

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Media Kit The Public Affairs Office is prepared to aid media representatives in preparing for Jefferson Lab stories and photo sessions. We can also help reporters find expert sources for science and technology-based stories. Media kits, provided upon request, offer a wealth of Jefferson Lab information including, photos, informational video, a virtual tour video, news clippings, maps and publications. This information also is available online. The Public Affairs Office makes every effort to work

  5. MeshKit

    Energy Science and Technology Software Center

    2010-10-05

    MeshKit is an open-source library of mesh generation functionality. MeshKit has general mesh manipulation and generation functions such as Copoy, Move, Rotate and Extrude mesh. In addition, new quad mesh and embedded boundary Cartesian mesh algorithm (EB Mesh) are included. Interfaces to several public domain meshing algorithms (TetGen, netgen, triangle, Gmsh, camal) are also offered. This library interacts with mesh data mostly through iMesh including accessing the mesh in parallel. It also can interact withmore » iGeom interface to provide geometry functionality such as importing solid model based geometries. iGeom and IMesh are implemented in the CGM and MOAB packages, respectively. For some non-existing function in iMesh such as tree-construction and ray-tracing, MeshKit also interacts with MOAB functions directly.« less

  6. Bioenergy 2015 Press Kit

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    5 Press Kit This U.S. Department of Energy Bioenergy 2015 Press Kit provides contacts and resources to media who cover conference-related news. Logistics Bioenergy 2015: Opportunities in a Changing Energy Landscape June 23-June 24 Walter E. Washington Convention Center 801 Mt. Vernon Place, NW Washington, D.C. 20001 Registration: Online registration: https://ww2.eventrebels.com/er/Registration/StepRegInfo.jsp?ActivityID=12553&StepNumber=1 Onsite registration: 7:00 a.m. to 8:00 a.m. EST both

  7. TEPP GoKit Information

    Energy.gov [DOE]

    Each TEPP Regional Coordinator has Instructor and Practical Exercise GoKits available for check out to assist instructors with delivery of the MERRTT Modules. The Instructor GoKit contains many of...

  8. Valencia West, Arizona: Energy Resources | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Hide Map This article is a stub. You can help OpenEI by expanding it. Valencia West is a census-designated place in Pima County, Arizona.1 References US...

  9. Valencia County, New Mexico: Energy Resources | Open Energy Informatio...

    OpenEI (Open Energy Information) [EERE & EIA]

    Zone Subtype B. Places in Valencia County, New Mexico Belen, New Mexico Bosque Farms, New Mexico Casa Colorada, New Mexico El Cerro-Monterey Park, New Mexico Jarales, New Mexico...

  10. Protein crystallography prescreen kit

    DOEpatents

    Segelke, Brent W.; Krupka, Heike I.; Rupp, Bernhard

    2007-10-02

    A kit for prescreening protein concentration for crystallization includes a multiplicity of vials, a multiplicity of pre-selected reagents, and a multiplicity of sample plates. The reagents and a corresponding multiplicity of samples of the protein in solutions of varying concentrations are placed on sample plates. The sample plates containing the reagents and samples are incubated. After incubation the sample plates are examined to determine which of the sample concentrations are too low and which the sample concentrations are too high. The sample concentrations that are optimal for protein crystallization are selected and used.

  11. Protein crystallography prescreen kit

    DOEpatents

    Segelke, Brent W.; Krupka, Heike I.; Rupp, Bernhard

    2005-07-12

    A kit for prescreening protein concentration for crystallization includes a multiplicity of vials, a multiplicity of pre-selected reagents, and a multiplicity of sample plates. The reagents and a corresponding multiplicity of samples of the protein in solutions of varying concentrations are placed on sample plates. The sample plates containing the reagents and samples are incubated. After incubation the sample plates are examined to determine which of the sample concentrations are too low and which the sample concentrations are too high. The sample concentrations that are optimal for protein crystallization are selected and used.

  12. ARC4461kit

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    talk about a Disaster Supplies Kit? After a disaster, local officials and relief workers will be on the scene, but they cannot reach everyone immediately. You could get help in hours, or it may take days. Basic services, such as electricity, gas, water, and tele- phones, may be cut off, or you may have to evacuate at a moment's notice. You probably won't have time to shop or search for the supplies you'll need. Your family will cope best by preparing for disaster before it strikes. What is a

  13. Press Kit Bionergy 2016

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Press Kit Bioenergy 2016: Mobilizing the Bioeconomy Through Innovation July 12-14, 2016 Walter E. Washington Convention Center 801 Mt. Vernon Place, NW Washington, D.C. 20001 Registration: Onsite registration starts at 11 a.m. Eastern Time on July 12 and 7 a.m. Eastern Time, July 13 and 14. Internet Access: Wireless Internet access is available in public spaces at the Convention Center. Event Overview In partnership with the Clean Energy Research and Education Foundation (www.ceref.org), this

  14. Optics learning through affordable kit

    SciTech Connect

    P, Anusha N E-mail: chitrashaji@gmail.com Shaji, Chitra E-mail: chitrashaji@gmail.com Sharan, Alok E-mail: chitrashaji@gmail.com

    2014-10-15

    An affordable kit which helps to understand some of the optical phenomena qualitatively and quantitatively is presented in this paper. It supplements optics taught in classes. The kit consists of equipments which are available in the market at nominal cost such as laser pointer, lenses, glass plates, razor blades, coins, ball bearing etc. Experiments which come under wave optics (interference and diffraction) and ray optics (reflection and refraction) are explained using this kit.

  15. Bioenergy 2016 Press Kit | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Press Kit Bioenergy 2016 Press Kit This U.S. Department of Energy Bioenergy 2016 Press Kit provides contacts and resources to media who cover conference-related news. bioenergy2016_press_kit.pdf (279.11 KB) More Documents & Publications Bioenergy 2015 Press Kit Federal Activities Report on the Bioeconomy WEBINAR: A CHANGING MARKET FOR BIOFUELS AND BIOPRODUCTS

  16. Kit Carson Wind Project | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Jump to: navigation, search Name Kit Carson Wind Project Facility Kit Carson Sector Wind energy Facility Type Community Wind Facility Status In Service Address 102 W 5th...

  17. Oncoprotein protein kinase antibody kit (Patent) | DOEPatents

    Office of Scientific and Technical Information (OSTI)

    Oncoprotein protein kinase antibody kit Title: Oncoprotein protein kinase antibody kit An ... domain and polynucleotide sequences and method of detection of JNK are provided herein. ...

  18. Bioenergy 2015 Press Kit | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Press Kit Bioenergy 2015 Press Kit This U.S. Department of Energy Bioenergy 2015 Press Kit provides contacts and resources to media who cover conference-related news. bioenergy_2015_press_kit.pdf (168.59 KB) More Documents & Publications Bioenergy 2016 Press Kit Rainbows and Leprechauns: Finding Gold in Partnerships (101) Energy Efficiency on Display: Using Demonstration Projects to Showcase Home Performance Opportunities (201)

  19. Solid phase microextraction field kit

    SciTech Connect

    Nunes, Peter J.; Andresen, Brian D.

    2005-08-16

    A field kit for the collection, isolation and concentration of trace amounts of high explosives (HE), biological weapons (BW) and chemical weapons (CW) residues in air, soil, vegetation, swipe, and liquid samples. The field kit includes a number of Solid Phase Microextraction (SPME) fiber and syringe assemblies in a hermetically sealed transportation container or tubes which includes a sampling port, a number of extra SPME fiber and syringe assemblies, the fiber and syringe assemblies including a protective cap for the fiber, and an extractor for the protective cap, along with other items including spare parts, protective glove, and an instruction manual, all located in an airtight container.

  20. UNEP Energy Resource Kit | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Tool Summary LAUNCH TOOL Name: UNEP Energy Resource Kit AgencyCompany Organization: United Nations Environment Programme Sector: Energy Focus Area: Energy Efficiency,...

  1. UNEP Climate Change Resource Kit | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Climate Change Resource Kit Jump to: navigation, search Tool Summary LAUNCH TOOL Name: UNEP Climate Change Resource Kit AgencyCompany Organization: United Nations Environment...

  2. Energy Conservation Standards for Ceiling Fan Light Kits Notice...

    Energy.gov [DOE] (indexed site)

    Kits notice of proposed rulemaking More Documents & Publications ISSUANCE 2015-06-25: Energy Conservation Program: Energy Conservation Standards for Ceiling Fan Light Kits,...

  3. Tool Kit Framework: Small Town Energy Program (STEP) | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Tool Kit Framework: Small Town Energy Program (STEP) Tool Kit Framework: Small Town Energy ... Outreach Letters to Engage Partner Communities, Letters of Understanding * Letters ...

  4. LEM-CF Premixed Tool Kit

    Energy Science and Technology Software Center

    2015-01-19

    The purpose of LEM-CF Premixed Tool Kit is to process premixed flame simulation data from the LEM-CF solver (https://fileshare.craft-tech.com/clusters/view/lem-cf) into a large-eddy simulation (LES) subgrid model database. These databases may be used with a user-defined-function (UDF) that is included in the Tool Kit. The subgrid model UDF may be used with the ANSYS FLUENT flow solver or other commercial flow solvers.

  5. Data Center Energy Efficiency Measurement Assesment Kit Guide and Specification

    Energy.gov [DOE]

    Guide describes the Federal Energy Management Program Data Center Energy Efficiency Measurement Assesment Kit and Specifications.

  6. Data Center Energy Efficiency Measurement Assessment Kit and Specifications

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    | Department of Energy Efficiency Measurement Assessment Kit and Specifications Data Center Energy Efficiency Measurement Assessment Kit and Specifications Guide describes the Federal Energy Management Program Data Center Energy Efficiency Measurement Assessment Kit and Specifications. Download the assessment kit and specifications. (909.16 KB) More Documents & Publications Wireless Sensors Improve Data Center Efficiency Energy Efficiency Opportunities in Federal High Performance

  7. SEP and EECBG program communications resource kit

    Energy.gov [DOE]

    A resource kit that helps a user draw attention to SEP and EECBG projects he/she has developed and showcase the related economic recovery, made possible through the 2009 Recovery Act. The toolkit provides guidance on effectively disseminating information about projects that employ citizens and transform the future of energy in the United States.

  8. EECBG Success Story: Classes, Kits and Energy Savings for Pinellas...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    EECBG Success Story: Classes, Kits and Energy Savings for Pinellas County September 15, 2010 - 5:18pm Addthis Residents of Pinellas County, Florida will receive energy kits with ...

  9. Microsoft Word - DOE_science_kits.docx

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    C M E T is (T T in S "I a S u a In th U.S. Carls Wast P.O. Carls CARLSBAD Mexico will h Energy's (DO The DOE ov s America's TRU) radioa The kits, targ ncluding nu Southeaster t encourag nd what the Science topi sing electri lso corresp n addition to he other kits Departmen sbad Field O te Isolation Box 3090 sbad, New M WIPP O For D, N.M., Jul have a lot to OE) Carlsb versees the s only deep active wast geted for th clear, but ta rn New Mex es the disc eir impact is ics include city from co

  10. Sample rotating turntable kit for infrared spectrometers

    DOEpatents

    Eckels, Joel Del (Livermore, CA); Klunder, Gregory L. (Oakland, CA)

    2008-03-04

    An infrared spectrometer sample rotating turntable kit has a rotatable sample cup containing the sample. The infrared spectrometer has an infrared spectrometer probe for analyzing the sample and the rotatable sample cup is adapted to receive the infrared spectrometer probe. A reflectance standard is located in the rotatable sample cup. A sleeve is positioned proximate the sample cup and adapted to receive the probe. A rotator rotates the rotatable sample cup. A battery is connected to the rotator.

  11. Data Center Energy Efficiency Measurement Assessment Kit Guide and Specification

    SciTech Connect

    2012-10-26

    A portable and temporary wireless mesh assessment kit can be used to speed up and reduce the costs of a data center energy use assessment and overcome the issues with respect to shutdowns. The assessment kit is comprised of temperature, relative humidity, and pressure sensors. Also included are power meters that can be installed on computer room air conditioners (CRACs) without intrusive interruption of data center operations. The assessment kit produces data required for a detailed energy assessment of the data center.

  12. FUELS IN SOIL TEST KIT: FIELD USE OF DIESEL DOG SOIL TEST KITS

    SciTech Connect

    Susan S. Sorini; John F. Schabron; Joseph F. Rovani, Jr.

    2002-09-30

    Western Research Institute (WRI) has developed a new commercial product ready for technology transfer, the Diesel Dog{reg_sign} Portable Soil Test Kit, for performing analysis of fuel-contaminated soils in the field. The technology consists of a method developed by WRI (U.S. Patents 5,561,065 and 5,976,883) and hardware developed by WRI that allows the method to be performed in the field (patent pending). The method is very simple and does not require the use of highly toxic reagents. The aromatic components in a soil extract are measured by absorption at 254 nm with a field-portable photometer. WRI added significant value to the technology by taking the method through the American Society for Testing and Materials (ASTM) approval and validation processes. The method is designated as ASTM Method D 5831-96, Standard Test Method for Screening Fuels in Soils. This ASTM designation allows the method to be used for federal compliance activities. In June 2001, the Diesel Dog technology won an American Chemical Society Regional Industrial Innovations Award. To gain field experience with the new technology, Diesel Dog kits have been used for a variety of site evaluation and cleanup activities. Information gained from these activities has led to improvements in hardware configurations and additional insight into correlating Diesel Dog results with results from laboratory methods. The Wyoming Department of Environmental Quality (DEQ) used Diesel Dog Soil Test Kits to guide cleanups at a variety of sites throughout the state. ENSR, of Acton, Massachusetts, used a Diesel Dog Portable Soil Test Kit to evaluate sites in the Virgin Islands and Georgia. ChemTrack and the U.S. Army Corps of Engineers successfully used a test kit to guide excavation at an abandoned FAA fuel-contaminated site near Fairbanks, Alaska. Barenco, Inc. is using a Diesel Dog Portable Soil Test Kit for site evaluations in Canada. A small spill of diesel fuel was cleaned up in Laramie, Wyoming using a Diesel

  13. Tool Kit Framework: Small Town University Energy Program (STEP)

    Energy.gov [DOE]

    Tool Kit Framework: Small Town University Energy Program (STEP), as posted on the U.S. Department of Energy's Better Buildings Neighborhood Program website.

  14. Data Center Energy Efficiency Measurement Assesment Kit Guide...

    Energy.gov [DOE] (indexed site)

    Data Center Energy Efficiency Measurement Assesment Kit and Specifications. dcassessmentkit.pdf (909.16 KB) More Documents & Publications Wireless Sensors Improve Data ...

  15. Data Center Energy Efficiency Measurement Assesment Kit Guide...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Data Center Energy Efficiency Measurement Assessment Kit Guide and Specification By Lawrence Berkeley National Laboratory Rod Mahdavi, PE, LEED AP October 26, 2012 Acknowledgements ...

  16. NREL: Workforce Development and Education Programs - Kit and Component

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Supplier List Kit and Component Supplier List Learn about available kits and component suppliers for NREL's Car Competitions, a classroom-based, hands-on educational program for 6th, 7th, and 8th grade students. Please contact suppliers for pricing. Junior Solar Sprint Suppliers Products Solar Made 2807 North Prospect Colorado Springs, CO 80907 Tel: 1-800-246-7012 Fax: 719-635-5398 Email: Sales@SolarMade.com Website: Solar Made JSS Kit Includes solar electric panel (3 Volts @ 3 watts),

  17. CALiPER: Troffers, Kits, and Tubes | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Webcasts » CALiPER: Troffers, Kits, and Tubes CALiPER: Troffers, Kits, and Tubes This June 20, 2013 webcast reviewed the recently completed CALiPER Study, Recessed Troffer Lighting, in which a group of lighting designers, engineers, and facility managers compared the performance of conventional fluorescent troffers with T8 LED tube retrofits, LED retrofit kits, and dedicated LED troffers. Troffers (1'×4', 2'×2', and 2'×4') comprise more than half of all luminaires installed in commercial

  18. Kit systems for granulated decontamination formulations

    DOEpatents

    Tucker, Mark D.

    2010-07-06

    A decontamination formulation and method of making that neutralizes the adverse health effects of both chemical and biological compounds, especially chemical warfare (CW) and biological warfare (BW) agents, and toxic industrial chemicals. The formulation provides solubilizing compounds that serve to effectively render the chemical and biological compounds, particularly CW and BW compounds, susceptible to attack, and at least one reactive compound that serves to attack (and detoxify or kill) the compound. The formulation includes at least one solubilizing agent, a reactive compound, a sorbent additive, and water. A highly adsorbent sorbent additive (e.g., amorphous silica, sorbitol, mannitol, etc.) is used to "dry out" one or more liquid ingredients into a dry, free-flowing powder that has an extended shelf life, and is more convenient to handle and mix in the field. The formulation can be pre-mixed and pre-packaged as a multi-part kit system, where one or more of the parts are packaged in a powdered, granulated form for ease of handling and mixing in the field.

  19. Classes, Kits and Energy Savings for Pinellas County | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Classes, Kits and Energy Savings for Pinellas County September 15, 2010 - 5:18pm Addthis Stephen Graff Former Writer & editor for Energy Empowers, EERE Officials of PEEP, the ...

  20. Tool Kit Framework: Small Town Energy Program (STEP)

    Energy.gov [DOE]

    The goal of Tool Kit Framework: Small Town University Energy Program (STEP) is to assemble all the key elements that went into the design and delivery of STEP—formerly STEP-UP, a University Park,...

  1. SU-E-T-343: Valencia Applicator Commissioning Using a Micro-Chamber Array

    SciTech Connect

    Carmona-Meseguer, V; Palomo-Llinares, R; Candela-Juan, C; Gimeno-Olmos, J; Lliso-Valverde, F; Garcia-Martinez, T; Richart-Sancho, J; Granero, D; Ballester, F; Perez-Calatayud, J

    2014-06-01

    Purpose: In the commissioning and QA of surface isotope-based applicators, source-indexer distance (SID) has a great influence in the flatness, symmetry and output. To these purposes, methods described in the literature are the use of a special insert at the entrance of dwell chamber or radiochromic films. Here we present the experience with a micro-chamber array to perform the commissioning and QA of Valencia applicators. Methods: Valencia applicators have been used, the classic and the new extra-shielded version. A micro-chamber array has been employed, 1000 SRS (PTW), with 977 liquid filled, 2.3×2.3×0.5 mm{sup 3} sized ion chambers covering 11×11 cm{sup 2}, which spacing is 2.5 mm in the central 5.5×5.5 cm{sup 2}, dedicated mainly in principle, in conjunction with Octavius 4D (PTW), to IMRT, VMAT, SBRT verifications. Verisoft software that allows for 3D and planar analysis has been used to evaluate the results. Applicators were located on the surface of the array. To verify the SID, measurements corresponding to the reference value, SID ± 1 mm and SID ± 2 mm were acquired (integration time was fixed in order to discard the influence of the source entrance/exit). Once SID was determined, standard protocol treatments corresponding to 3 Gy and 7 Gy were acquired in order to establish typical patient dose distribution. Results: The method is fast and sensitive. The SID obtained was 1321 mm which is the nominal value included in the applicator manual. For example at 1319 mm an asymmetry of ±8% with respect to the central value was measured, along with a central deviation of −4% referred to 1321 mm. Conclusion: A practical method for the commissioning and QA of Valencia applicators has been described. It has been shown that it is an efficient and accurate tool for these purposes as well as for the verification of the absolute output constancy.

  2. Remote Alaskan Communities Energy Efficiency Competition Social Media Kit |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy Remote Alaskan Communities Energy Efficiency Competition Social Media Kit Remote Alaskan Communities Energy Efficiency Competition Social Media Kit Alaska Stakeholders-Below you will find some pre-written Facebook post suggestions for getting the word out about the Remote Alaskan Communities Energy Efficiency Competition to communities. Please check back throughout the month for more updates. RACEE Facebook Posts svs.docx (14.11 KB) More Documents & Publications FACT

  3. Evaluation of models for estimating solar irradiation on vertical surfaces at Valencia, Spain

    SciTech Connect

    Utrillas, M.P.; Martinez-Lozano, J.A.; Casanovas, A.J. )

    1991-01-01

    Hourly irradiation data recorded on vertical surfaces at north, east, south, and west orientations during the winter period going from December 1989 to March 1990 in Valencia, Spain, have been compared with estimated solar irradiation from several tilted-surface models. The isotropic-, Temps' and Coulson's-, Klucher's-, Hay's-, Shartveit's and Olseth's, Gueymard's-, and Perez' (simplified) models have been considered for this comparison. Root-mean-square-difference (RMSD), mean-bias-difference (MBD) and mean-absolute-difference (MAD) estimators have been used to measure the departure of models from experimental data. Modeled values are evaluated with the original coefficients proposed by the authors. Results of comparison show that the south orientation is less prone to modeling errors. Perez' 25{degree} circumsolar simplified model being the less affected by errors in north, eat and south orientations; for west orientation Klucher's model gives a lesser error. These results are consistent with those reported in previous studies.

  4. MEMS Pro Design Kit - Parts A, B, and C

    Energy Science and Technology Software Center

    2006-06-15

    Part A: SUMMiT V design Kit components for use with MEMS Pro from SoftMEMS Part B: SUMMiT V remote DRC and gear generator source code for use with autocad visual basic Part C: SUMMiT V DRC rules source and test cases for Calibre DRC engine

  5. Documentation for MeshKit - Reactor Geometry (&mesh) Generator

    SciTech Connect

    Jain, Rajeev; Mahadevan, Vijay

    2015-09-30

    This report gives documentation for using MeshKit’s Reactor Geometry (and mesh) Generator (RGG) GUI and also briefly documents other algorithms and tools available in MeshKit. RGG is a program designed to aid in modeling and meshing of complex/large hexagonal and rectilinear reactor cores. RGG uses Argonne’s SIGMA interfaces, Qt and VTK to produce an intuitive user interface. By integrating a 3D view of the reactor with the meshing tools and combining them into one user interface, RGG streamlines the task of preparing a simulation mesh and enables real-time feedback that reduces accidental scripting mistakes that could waste hours of meshing. RGG interfaces with MeshKit tools to consolidate the meshing process, meaning that going from model to mesh is as easy as a button click. This report is designed to explain RGG v 2.0 interface and provide users with the knowledge and skills to pilot RGG successfully. Brief documentation of MeshKit source code, tools and other algorithms available are also presented for developers to extend and add new algorithms to MeshKit. RGG tools work in serial and parallel and have been used to model complex reactor core models consisting of conical pins, load pads, several thousands of axially varying material properties of instrumentation pins and other interstices meshes.

  6. Buyer's guide to sunspace kits and plans. [Greenhouses for growing, heat collecting, and living space

    SciTech Connect

    Smolen, M.

    1983-09-01

    Numerous manufacturers have succeeded in preparing do-it-yourself sunspace kits. In order to eliminate some of the confusion, a chart listing 35 manufacturers is presented, along with helpful information concerning each kit.

  7. 2014-10-27 Issuance: Test Procedure for Ceiling Fan Light Kits...

    Energy Saver

    7 Issuance: Test Procedure for Ceiling Fan Light Kits; Notice of Proposed Rulemaking 2014-10-27 Issuance: Test Procedure for Ceiling Fan Light Kits; Notice of Proposed Rulemaking ...

  8. WPN 12-4: Weatherization Assistance Program Financial Management Training Tool Kit

    Office of Energy Efficiency and Renewable Energy (EERE)

    To issue the Financial Management Training Tool Kit to all Grantee for use in the Weatherization Assistance Program.

  9. NSTX-U Press Kit | Princeton Plasma Physics Lab

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Overview Experimental Fusion Research Theoretical Fusion Research Basic Plasma Science Plasma Astrophysics Other Physics and Engineering Research PPPL Technical Reports NSTX-U Education Organization Contact Us Overview Experimental Fusion Research Theoretical Fusion Research Basic Plasma Science Plasma Astrophysics Other Physics and Engineering Research PPPL Technical Reports NSTX-U NSTX-U Press Kit Upgraded NSTX center stack. (photo by Elle Starkman/ PPPL Office of Communications The NSTX-U

  10. ISSUANCE 2015-06-25: Energy Conservation Program: Energy Conservation Standards for Ceiling Fan Light Kits, Notice of Proposed Rulemaking

    Energy.gov [DOE]

    Energy Conservation Program: Energy Conservation Standards for Ceiling Fan Light Kits, Notice of Proposed Rulemaking

  11. Enforcement Policy: Certain Ceiling Fans and Certain Ceiling Fan Light Kits

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    | Department of Energy Certain Ceiling Fans and Certain Ceiling Fan Light Kits Enforcement Policy: Certain Ceiling Fans and Certain Ceiling Fan Light Kits Issued: December 15, 2015 Revised: March 15, 2016 DOE announced an enforcement policy in a Federal Register notice published on December 24, 2015. This document provides additional information about how DOE plans to implement the policy. This policy is limited to certain ceiling fans and certain ceiling fan light kits manufactured prior to

  12. Kit for providing a technetium medical radioimaging agent

    DOEpatents

    Wildung, Raymond E.; Garland, Thomas R.; Li, Shu-Mei W.

    2000-01-01

    The present invention is directed toward a kit for microbial reduction of a technetium compound to form other compounds of value in medical imaging. The technetium compound is combined in a mixture with non-growing microbial cells which contain a technetium-reducing enzyme system, a stabilizing agent and an electron donor in a saline solution under anaerobic conditions. The mixture is substantially free of an inorganic technetium reducing agent and its reduction products. The resulting product is Tc of lower oxidation states, the form of which can be partially controlled by the stabilizing agent. It has been discovered that the microorganisms Shewanella alga, strain Bry and Shewanella putrifacians, strain CN-32 contain the necessary enzyme systems for technetium reduction and can form both mono nuclear and polynuclear reduced Tc species depending on the stabilizing agent.

  13. Kit for detecting nucleic acid sequences using competitive hybridization probes

    DOEpatents

    Lucas, Joe N.; Straume, Tore; Bogen, Kenneth T.

    2001-01-01

    A kit is provided for detecting a target nucleic acid sequence in a sample, the kit comprising: a first hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a first portion of the target sequence, the first hybridization probe including a first complexing agent for forming a binding pair with a second complexing agent; and a second hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a second portion of the target sequence to which the first hybridization probe does not selectively hybridize, the second hybridization probe including a detectable marker; a third hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a first portion of the target sequence, the third hybridization probe including the same detectable marker as the second hybridization probe; and a fourth hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a second portion of the target sequence to which the third hybridization probe does not selectively hybridize, the fourth hybridization probe including the first complexing agent for forming a binding pair with the second complexing agent; wherein the first and second hybridization probes are capable of simultaneously hybridizing to the target sequence and the third and fourth hybridization probes are capable of simultaneously hybridizing to the target sequence, the detectable marker is not present on the first or fourth hybridization probes and the first, second, third, and fourth hybridization probes each include a competitive nucleic acid sequence which is sufficiently complementary to a third portion of the target sequence that the competitive sequences of the first, second, third, and fourth hybridization probes compete with each other to hybridize to the third portion of the

  14. Stem cell factor (SCF) protects osteoblasts from oxidative stress through activating c-Kit-Akt signaling

    SciTech Connect

    Yang, Lei; Wu, Zhong; Yin, Gang; Liu, Haifeng; Guan, Xiaojun; Zhao, Xiaoqiang; Wang, Jianguang; Zhu, Jianguo

    2014-12-12

    Highlights: • SCF receptor c-Kit is functionally expressed in primary and transformed osteoblasts. • SCF protects primary and transformed osteoblasts from H{sub 2}O{sub 2}. • SCF activation of c-Kit in osteoblasts, required for its cyto-protective effects. • c-Kit mediates SCF-induced Akt activation in cultured osteoblasts. • Akt activation is required for SCF-regulated cyto-protective effects in osteoblasts. - Abstract: Osteoblasts regulate bone formation and remodeling, and are main target cells of oxidative stress in the progression of osteonecrosis. The stem cell factor (SCF)-c-Kit pathway plays important roles in the proliferation, differentiation and survival in a range of cell types, but little is known about its functions in osteoblasts. In this study, we found that c-Kit is functionally expressed in both osteoblastic-like MC3T3-E1 cells and primary murine osteoblasts. Its ligand SCF exerted significant cyto-protective effects against hydrogen peroxide (H{sub 2}O{sub 2}). SCF activated its receptor c-Kit in osteoblasts, which was required for its cyto-protective effects against H{sub 2}O{sub 2}. Pharmacological inhibition (by Imatinib and Dasatinib) or shRNA-mediated knockdown of c-Kit thus inhibited SCF-mediated osteoblast protection. Further investigations showed that protection by SCF against H{sub 2}O{sub 2} was mediated via activation of c-Kit-dependent Akt pathway. Inhibition of Akt activation, through pharmacological or genetic means, suppressed SCF-mediated anti-H{sub 2}O{sub 2} activity in osteoblasts. In summary, we have identified a new SCF-c-Kit-Akt physiologic pathway that protects osteoblasts from H{sub 2}O{sub 2}-induced damages, and might minimize the risk of osteonecrosis caused by oxidative stress.

  15. Text-Alternative Version: CALiPER: Troffers, Kits, and Tubes Webcast |

    Energy Saver

    Department of Energy CALiPER: Troffers, Kits, and Tubes Webcast Text-Alternative Version: CALiPER: Troffers, Kits, and Tubes Webcast Below is the text-alternative version of the "CALiPER: Troffers, Kits, and Tubes" webcast, held June 20, 2013. Linda Sandahl: So we're very happy to have as our speaker today, Naomi Miller of Pacific Northwest National Laboratory. Naomi is an engineer in the Solid-State Lighting Program here at PNNL and she focuses on lighting quality, energy

  16. DAKOTA Design Analysis Kit for Optimization and Terascale

    Energy Science and Technology Software Center

    2010-02-24

    The DAKOTA (Design Analysis Kit for Optimization and Terascale Applications) toolkit provides a flexible and extensible interface between simulation codes (computational models) and iterative analysis methods. By employing object-oriented design to implement abstractions of the key components required for iterative systems analyses, the DAKOTA toolkit provides a flexible and extensible problem-solving environment for design and analysis of computational models on high performance computers.A user provides a set of DAKOTA commands in an input file andmore » launches DAKOTA. DAKOTA invokes instances of the computational models, collects their results, and performs systems analyses. DAKOTA contains algorithms for optimization with gradient and nongradient-based methods; uncertainty quantification with sampling, reliability, polynomial chaos, stochastic collocation, and epistemic methods; parameter estimation with nonlinear least squares methods; and sensitivity/variance analysis with design of experiments and parameter study methods. These capabilities may be used on their own or as components within advanced strategies such as hybrid optimization, surrogate-based optimization, mixed integer nonlinear programming, or optimization under uncertainty. Services for parallel computing, simulation interfacing, approximation modeling, fault tolerance, restart, and graphics are also included.« less

  17. EECBG Success Story: Classes, Kits and Energy Savings for Pinellas County

    Energy.gov [DOE]

    Residents of Pinellas County, Florida will receive energy kits with weather stripping, low-flow shower heads, and window insulators once they attend a special class, courtesy of the Energy Efficiency and Conservation Block Grant. Learn more.

  18. Evaluation of aftermarket LPG conversion kits in light-duty vehicle applications. Final report

    SciTech Connect

    Bass, E.A.

    1993-06-01

    SwRI was contracted by NREL to evaluate three LPG conversion kits on a Chevrolet Lumina. The objective of the project was to measure the Federal Test Procedure (FTP) emissions and fuel economy of these kits, and compare their performance to gasoline-fueled operation and to each other. Varying LPG fuel blends allowed a preliminary look at the potential for fuel system disturbance. The project required kit installation and adjustment according to manufacturer`s instructions. A limited amount of trouble diagnosis was also performed on the fuel systems. A simultaneous contract from the Texas Railroad Commission, in cooperation with NREL, provided funds for additional testing with market fuels (HD5 propane and industry average gasoline) and hydrocarbon (HC) emissions speciation to determine the ozone-forming potential of LPG HC emissions. This report documents the procurement, installation, and testing of these LPG conversion kits.

  19. LANL12-RS-108J Device Modeler Tool Kit - DMTK Final Report for...

    Office of Scientific and Technical Information (OSTI)

    Title: LANL12-RS-108J Device Modeler Tool Kit - DMTK Final Report for FY14 Authors: Temple, Brian Allen 1 ; Pimentel, David A. 1 + Show Author Affiliations Los Alamos National ...

  20. Step by Step Tool Kit for Local Governments to Go Solar | Open...

    OpenEI (Open Energy Information) [EERE & EIA]

    to Go Solar1 Overview "This NSHP Municipal Tool Kit is one of the many ways the Energy Commission is working together with local governments across our state. The Tool...

  1. V-109: Google Chrome WebKit Type Confusion Error Lets Remote...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Google Chrome WebKit Type Confusion Error Lets Remote Users Execute Arbitrary Code PLATFORM: Google Chrome prior to 25.0.1364.160 ABSTRACT: A vulnerability was reported in...

  2. An evaluation of chemical screening test kits for lead in paint

    SciTech Connect

    Oglesby, L.S.

    1996-04-01

    The Residential Lead-Based Paint Hazard Reduction Act (Title X) requires abatement and management of lead-based paint. The purpose of this study was to evaluate three chemical screening test kits using materials and methods from one study and subjecting the results to the statistical analysis of another. The three kits were used to predict the presence of lead in paint at ten weight concentrations from 0.04 to 3.97%. Paint was applied to four wood boards yielding a sample size of 40. Four boards were painted with lead-free paint and used as blanks. All of the boards were tested with the three test kits by an untrained individual having no knowledge of the actual lead content. Sensitivity, specificity, and false positive and negative rates were calculated for the test kit results. The manufactures` detection limits, the observed sensitivity ranged from 1.00 to 0.80, specificity ranged from 1.00 to 0.42, false positive ranged from 0 to 58%, and false negatives ranged from 0 to 20%. At the 0.5% Federal threshold level, the observed sensitivity ranged from 1.00 to 0.94, specificity ranged from 1.00 to 0.5, false positives ranged from 0 to 11.1%, and false negatives ranged from 0 to 20%. The observed false positive and false negative rates for all three kits were found to be significantly lower than those reported in a previous study. These results indicate that the kits perform very well at the Federal threshold, with two of the kits having false negative rates below 12.5% and false positive rates of 3.13%. These results indicate that these two kits would probably be acceptable screening tests for lead in paint.

  3. Studies on KIT-6 Supported Cobalt Catalyst for Fischer–Tropsch Synthesis

    SciTech Connect

    Gnanamani, M.; Jacobs, G; Graham, U; Ma, W; Pendyala, V; Ribeiro, M; Davis, B

    2010-01-01

    KIT-6 molecular sieve was used as a support to prepare cobalt catalyst for Fischer-Tropsch synthesis (FTS) using an incipient wetness impregnation method to produce cobalt loadings of 15 and 25 wt%. The catalysts were characterized by BET surface area, X-ray diffraction, scanning transmission election microscopy (STEM), extended X-ray absorption fine spectroscopy and X-ray absorption near edge spectroscopy. The catalytic properties for FTS were evaluated using a 1L CSTR reactor. XRD, pore size distribution, and STEM analysis indicate that the KIT-6 mesostructure remains stable during and after cobalt impregnation and tends to form smaller cobalt particles, probably located inside the mesopores. The mesoporous KIT-6 exhibited a slightly higher cobalt dispersion compared to amorphous SiO{sub 2} supported catalyst. With the higher Co loading (25 wt%) on KIT-6, partial structural collapse was observed after the FTS reaction. Compared to an amorphous SiO{sub 2} supported cobalt catalyst, KIT-6 supported cobalt catalyst displayed higher methane selectivity at a similar Co loading, likely due to diffusion effects.

  4. Kit for the rapid preparation of .sup.99m Tc red blood cells

    DOEpatents

    Richards, Powell; Smith, Terry D.

    1976-01-01

    A method and sample kit for the preparation of .sup.99m Tc-labeled red blood cells in a closed, sterile system. A partially evacuated tube, containing a freeze-dried stannous citrate formulation with heparin as an anticoagulant, allows whole blood to be automatically drawn from the patient. The radioisotope is added at the end of the labeling sequence to minimize operator exposure. Consistent 97% yields in 20 minutes are obtained with small blood samples. Freeze-dried kits have remained stable after five months.

  5. Methods, compositions and kits for imaging cells and tissues using nanoparticles and spatial frequency heterodyne imaging

    DOEpatents

    Rose-Petruck, Christoph; Wands, Jack R.; Rand, Danielle; Derdak, Zoltan; Ortiz, Vivian

    2016-04-19

    Methods, compositions, systems, devices and kits are provided herein for preparing and using a nanoparticle composition and spatial frequency heterodyne imaging for visualizing cells or tissues. In various embodiments, the nanoparticle composition includes at least one of: a nanoparticle, a polymer layer, and a binding agent, such that the polymer layer coats the nanoparticle and is for example a polyethylene glycol, a polyelectrolyte, an anionic polymer, or a cationic polymer, and such that the binding agent that specifically binds the cells or the tissue. Methods, compositions, systems, devices and kits are provided for identifying potential therapeutic agents in a model using the nanoparticle composition and spatial frequency heterodyne imaging.

  6. A Step by Step Tool Kit for Local Governments to Go Solar

    Energy.gov [DOE]

    This tool kit provides an array of strategies and options that local governments can implement to help encourage solar developments in their region, including amending general plans, incentivizing energy efficiency measures and solar installations, and educating local home builders about existing solar incentives. It also includes model ordinances and resolutions to help local governments promote solar development.

  7. Kit for the selective labeling of red blood cells in whole blood with [sup 99]Tc

    DOEpatents

    Srivastava, S.C.; Babich, J.W.; Straub, R.; Richards, P.

    1992-05-26

    Disclosed herein are a method and kit for the preparation of [sup 99m]Tc labeled red blood cells using whole blood in a closed sterile system containing stannous tin in a form such that it will enter the red blood cells and be available therein for reduction of technetium. No Drawings

  8. Kit for the selective labeling of red blood cells in whole blood with .sup.9 TC

    DOEpatents

    Srivastava, Suresh C.; Babich, John W.; Straub, Rita; Richards, Powell

    1992-01-01

    Disclosed herein are a method and kit for the preparation of .sup.99m Tc labeled red blood cells using whole blood in a closed sterile system containing stannous tin in a form such that it will enter the red blood cells and be available therein for reduction of technetium.

  9. Effects of supplemental feeding on survivorship, reproduction, and dispersal in San Joaquin kit foxes

    SciTech Connect

    Not Available

    1993-02-01

    Previous field studies at the Naval Petroleum Reserves in California indicated that a decline in tie population size of the endangered San Joaquin kit fox might be linked to declining prey abundance. To evaluate whether kit fox populations we limited by food resources; survival probabilities, sources of mortality, reproductive success, and dispersal rates were compared between foxes with access to supplemental food and foxes without access to supplemental food (controls). Of foxes born in 1988, the probabilities of supplementary fed foxes surviving to age one and age two were higher than corresponding probabilities of control foxes. Survival probabilities of fed foxes from the 1988 cohort also were higher than the average survival probabilities of foxes born in the previous eight years. Most foxes that died during their first year of life died in June, July, or August. Monthly probabilities of survival were higher for fed pups than control pups curing the months of July and August of 1988. Survival probabilities of fed foxes originally r captured as adults and fed foxes born in 1989 were not significantly different than survival probabilities of corresponding control groups. Most foxes for which a cause of death could be determined were lolled by predators. Average dispersal distances were not significantly different between fed and control groups but the two longest dispersal distances were made by control foxes. These results indicate that food availability affects survival, reproduction, and dispersal by kit foxes and provides evidence that kit fox populations may at times be limited by food abundance.

  10. Update on Development of Mesh Generation Algorithms in MeshKit

    SciTech Connect

    Jain, Rajeev; Vanderzee, Evan; Mahadevan, Vijay

    2015-09-30

    MeshKit uses a graph-based design for coding all its meshing algorithms, which includes the Reactor Geometry (and mesh) Generation (RGG) algorithms. This report highlights the developmental updates of all the algorithms, results and future work. Parallel versions of algorithms, documentation and performance results are reported. RGG GUI design was updated to incorporate new features requested by the users; boundary layer generation and parallel RGG support were added to the GUI. Key contributions to the release, upgrade and maintenance of other SIGMA1 libraries (CGM and MOAB) were made. Several fundamental meshing algorithms for creating a robust parallel meshing pipeline in MeshKit are under development. Results and current status of automated, open-source and high quality nuclear reactor assembly mesh generation algorithms such as trimesher, quadmesher, interval matching and multi-sweeper are reported.

  11. Photovoltaic module kit including connector assembly for non-penetrating array installation

    DOEpatents

    Botkin, Jonathan; Graves, Simon; Danning, Matt; Culligan, Matthew

    2011-11-22

    A PV module kit for non-penetrating rooftop installation, including a plurality of PV modules and a plurality of connectors. Each of the PV modules includes a PV laminate and a frame forming a mounting region assembled thereto. The connectors include a male connector having a male fastener extending from a head, and a female connector having a female fastener assembled within a head. The heads are entirely formed of plastic. The kit provides a mounted array state including a junction at which the mounting region of at least two of the PV modules are aligned and interconnected by engagement of the male connector with the female connector. The so-formed junction is substantially electrically insulated. The plurality of connectors can further include a spacer connector including a head forming a bore sized to slidably receive the male fastener, with all of the connector heads being identical.

  12. Photovoltaic module kit including connector assembly for non-penetrating array installation

    DOEpatents

    Botkin, Jonathan; Graves, Simon; Danning, Matt; Culligan, Matthew

    2012-10-23

    A PV module kit for non-penetrating rooftop installation, including a plurality of PV modules and a plurality of connectors. Each of the PV modules includes a PV laminate and a frame forming a mounting region assembled thereto. The connectors include a male connector having a male fastener extending from a head, and a female connector having a female fastener assembled within a head. The heads are entirely formed of plastic. The kit provides a mounted array state including a junction at which the mounting region of at least two of the PV modules are aligned and interconnected by engagement of the male connector with the female connector. The so-formed junction is substantially electrically insulated. The plurality of connectors can further include a spacer connector including a head forming a bore sized to slidably receive the male fastener, with all of the connector heads being identical.

  13. Photovoltaic module kit including connector assembly for non-penetrating array installation

    DOEpatents

    Botkin, Jonathan; Graves, Simon; Danning, Matt; Culligan, Matthew

    2013-12-31

    A PV module kit for non-penetrating rooftop installation, including a plurality of PV modules and a plurality of connectors. Each of the PV modules includes a PV laminate and a frame forming a mounting region assembled thereto. The connectors include a male connector having a male fastener extending from a head, and a female connector having a female fastener assempbled within a head. The heads are entirely formed of plastic. The kit provides a mounted array state including a junction at which the mounting regions of at least two of the PV modules are aligned and interconnected by engagement of the male connector with the female connector. The so-formed junction is substantially electrically insulated. The plurality of connectors can further include a spacer connector including a head forming a bore sized slidably receive the male fastener, with all of the connector heads being identical.

  14. Instantaneous radioiodination of rose bengal at room temperature and a cold kit therefor

    DOEpatents

    O'Brien, Jr., Harold A.; Hupf, Homer B.; Wanek, Philip M.

    1981-01-01

    The disclosure relates to the radioiodination of rose bengal at room temperature and a cold-kit therefor. A purified rose bengal tablet is stirred into acidified ethanol at or near room temperature, until a suspension forms. Reductant-free .sup.125 I.sup.- is added and the resulting mixture stands until the exchange label reaction occurs at room temperature. A solution of sterile isotonic phosphate buffer and sodium hydroxide is added and the final resulting mixture is sterilized by filtration.

  15. T-529: Apple Mac OS PackageKit Distribution Script Remote Code Execution Vulnerability

    Energy.gov [DOE]

    A format string issue exists in PackageKit's handling of distribution scripts. A man-in-the-middle attacker may be able to cause an unexpected application termination or arbitrary code execution when Software Update checks for new updates. This issue is addressed through improved validation of distribution scripts. This issue does not affect systems prior to Mac OS X v10.6.

  16. Field kit and method for testing for the presence of gunshot residue

    DOEpatents

    Rodacy, Philip J.; Walker, Pamela K.

    2003-09-02

    A field test kit for gunshot residue comprises a container having at least compartments separated by a barrier. A surface is tested by wiping it with a swab and placing the swab in a first compartment. The barrier is then breached, permitting reagent in the second compartment to flow onto the swab. The first compartment is transparent, and a color change will be observed if the reagent reacts with gunshot residue.

  17. CD45{sup low}c-Kit{sup high} cells have hematopoietic properties in the mouse aorta-gonad-mesonephros region

    SciTech Connect

    Nobuhisa, Ikuo; Department of Cell Fate Modulation, Institute of Molecular Embryology and Genetics Yamasaki, Shoutarou; Department of Cell Fate Modulation, Institute of Molecular Embryology and Genetics Taga, Tetsuya; Department of Cell Fate Modulation, Institute of Molecular Embryology and Genetics/Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, 860-0811

    2012-04-01

    Long-term reconstituting hematopoietic stem cells first arise from the aorta of the aorta-gonad-mesonephros (AGM) region in a mouse embryo. We have previously reported that in cultures of the dispersed AGM region, CD45{sup low}c-Kit{sup +} cells possess the ability to reconstitute multilineage hematopoietic cells, but investigations are needed to show that this is not a cultured artifact and to clarify when and how this population is present. Based on the expression profile of CD45 and c-Kit in freshly dissociated AGM cells from embryonic day 9.5 (E9.5) to E12.5 and aorta cells in the AGM from E13.5 to E15.5, we defined six cell populations (CD45{sup -}c-Kit{sup -}, CD45{sup -}c-Kit{sup low}, CD45{sup -}c-Kit{sup high}, CD45{sup low}c-Kit{sup high}, CD45{sup high}c-Kit{sup high}, and CD45{sup high}c-Kit{sup very} {sup low}). Among these six populations, CD45{sup low}c-Kit{sup high} cells were most able to form hematopoietic cell colonies, but their ability decreased after E11.5 and was undetectable at E13.5 and later. The CD45{sup low}c-Kit{sup high} cells showed multipotency in vitro. We demonstrated further enrichment of hematopoietic activity in the Hoechst dye-effluxing side population among the CD45{sup low}c-Kit{sup high} cells. Here, we determined that CD45{sup low}c-Kit{sup high} cells arise from the lateral plate mesoderm using embryonic stem cell-derived differentiation system. In conclusion, CD45{sup low}c-Kit{sup high} cells are the major hematopoietic cells of mouse AGM.

  18. Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer

    DOEpatents

    Kwok, Pui-Yan; Chen, Xiangning

    1999-01-01

    A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.

  19. Fuel Economy and Emissions of a Vehicle Equipped with an Aftermarket Flexible-Fuel Conversion Kit

    SciTech Connect

    Thomas, John F; Huff, Shean P; West, Brian H

    2012-04-01

    The U.S. Environmental Protection Agency (EPA) grants Certificates of Conformity for alternative fuel conversion systems and also offers other forms of premarket registration of conversion kits for use in vehicles more than two model years old. Use of alternative fuels such as ethanol, natural gas, and propane are encouraged by the Energy Policy Act of 1992. Several original equipment manufacturers (OEMs) produce emissions-certified vehicles capable of using alternative fuels, and several alternative fuel conversion system manufacturers produce EPA-approved conversion systems for a variety of alternative fuels and vehicle types. To date, only one manufacturer (Flex Fuel U.S.) has received EPA certifications for ethanol fuel (E85) conversion kits. This report details an independent evaluation of a vehicle with a legal installation of a Flex Fuel U.S. conversion kit. A 2006 Dodge Charger was baseline tested with ethanol-free certification gasoline (E0) and E20 (gasoline with 20 vol % ethanol), converted to flex-fuel operation via installation of a Flex Box Smart Kit from Flex Fuel U.S., and retested with E0, E20, E50, and E81. Test cycles included the Federal Test Procedure (FTP or city cycle), the highway fuel economy test (HFET), and the US06 test (aggressive driving test). Averaged test results show that the vehicle was emissions compliant on E0 in the OEM condition (before conversion) and compliant on all test fuels after conversion. Average nitrogen oxide (NOx) emissions exceeded the Tier 2/Bin 5 intermediate life NO{sub X} standard with E20 fuel in the OEM condition due to two of three test results exceeding this standard [note that E20 is not a legal fuel for non-flexible-fuel vehicles (non-FFVs)]. In addition, one E0 test result before conversion and one E20 test result after conversion exceeded the NOX standard, although the average result in these two cases was below the standard. Emissions of ethanol and acetaldehyde increased with increasing ethanol

  20. 2014-10-27 Issuance: Test Procedure for Ceiling Fan Light Kits; Notice of Proposed Rulemaking

    Energy.gov [DOE]

    This document is a pre-publication Federal Register notice of proposed rulemaking regarding test procedures for ceiling fan light kits, as issued by the Deputy Assistant Secretary for Energy Efficiency on October 27, 2014. Though it is not intended or expected, should any discrepancy occur between the document posted here and the document published in the Federal Register, the Federal Register publication controls. This document is being made available through the Internet solely as a means to facilitate the public's access to this document.

  1. STOCK: Structure mapper and online coarse-graining kit for molecular simulations

    SciTech Connect

    Bevc, Staš; Junghans, Christoph; Praprotnik, Matej

    2015-03-15

    We present a web toolkit STructure mapper and Online Coarse-graining Kit for setting up coarse-grained molecular simulations. The kit consists of two tools: structure mapping and Boltzmann inversion tools. The aim of the first tool is to define a molecular mapping from high, e.g. all-atom, to low, i.e. coarse-grained, resolution. Using a graphical user interface it generates input files, which are compatible with standard coarse-graining packages, e.g. VOTCA and DL_CGMAP. Our second tool generates effective potentials for coarse-grained simulations preserving the structural properties, e.g. radial distribution functions, of the underlying higher resolution model. The required distribution functions can be provided by any simulation package. Simulations are performed on a local machine and only the distributions are uploaded to the server. The applicability of the toolkit is validated by mapping atomistic pentane and polyalanine molecules to a coarse-grained representation. Effective potentials are derived for systems of TIP3P (transferable intermolecular potential 3 point) water molecules and salt solution. The presented coarse-graining web toolkit is available at http://stock.cmm.ki.si.

  2. STOCK: Structure mapper and online coarse-graining kit for molecular simulations

    DOE PAGES [OSTI]

    Bevc, Staš; Junghans, Christoph; Praprotnik, Matej

    2015-03-15

    We present a web toolkit STructure mapper and Online Coarse-graining Kit for setting up coarse-grained molecular simulations. The kit consists of two tools: structure mapping and Boltzmann inversion tools. The aim of the first tool is to define a molecular mapping from high, e.g. all-atom, to low, i.e. coarse-grained, resolution. Using a graphical user interface it generates input files, which are compatible with standard coarse-graining packages, e.g. VOTCA and DL_CGMAP. Our second tool generates effective potentials for coarse-grained simulations preserving the structural properties, e.g. radial distribution functions, of the underlying higher resolution model. The required distribution functions can be providedmore » by any simulation package. Simulations are performed on a local machine and only the distributions are uploaded to the server. The applicability of the toolkit is validated by mapping atomistic pentane and polyalanine molecules to a coarse-grained representation. Effective potentials are derived for systems of TIP3P (transferable intermolecular potential 3 point) water molecules and salt solution. The presented coarse-graining web toolkit is available at http://stock.cmm.ki.si.« less

  3. Methods and kits for predicting a response to an erythropoietic agent

    DOEpatents

    Merchant, Michael L.; Klein, Jon B.; Brier, Michael E.; Gaweda, Adam E.

    2015-06-16

    Methods for predicting a response to an erythropoietic agent in a subject include providing a biological sample from the subject, and determining an amount in the sample of at least one peptide selected from the group consisting of SEQ ID NOS: 1-17. If there is a measurable difference in the amount of the at least one peptide in the sample, when compared to a control level of the same peptide, the subject is then predicted to have a good response or a poor response to the erythropoietic agent. Kits for predicting a response to an erythropoietic agent are further provided and include one or more antibodies, or fragments thereof, that specifically recognize a peptide of SEQ ID NOS: 1-17.

  4. Method and kit for the selective labeling of red blood cells in whole blood with TC-99M

    DOEpatents

    Srivastava, Suresh C.; Babich, John W.; Straub, Rita; Richards, Powell

    1988-01-01

    Disclosed herein are a method and kit for the preparation of .sup.99m Tc labeled red blood cells using whole blood in a closed sterile system containing stannous tin in a form such that it will enter the red blood cells and be available therein for the reduction of technetium.

  5. Method and kit for the selective labeling of red blood cells in whole blood with Tc-99m

    DOEpatents

    Srivastava, S.C.; Babich, J.W.; Straub, R.; Richards, P.

    1988-07-05

    Disclosed herein are a method and kit for the preparation of [sup 99m]Tc labeled red blood cells using whole blood in a closed sterile system containing stannous tin in a form such that it will enter the red blood cells and be available for the reduction of technetium. No Drawings

  6. Instantaneous radioiodination of rose bengal at room temperature and a cold-kit therefor. [DOE patent application

    DOEpatents

    O'Brien, H. Jr.; Hupf, H.B.; Wanek, P.M.

    The disclosure relates to the radioiodination of rose bengal at room temperature and a cold-kit therefor. A purified rose bengal tablet is stirred into acidified ethanol at or near room temperature, until a suspension forms. Reductant-free /sup 125/I/sup -/ is added and the resulting mixture stands until the exchange label reaction occurs at room temperature. A solution of sterile isotonic phosphate buffer and sodium hydroxide is added and the final resulting mixture is sterilized by filtration.

  7. E-KIT: An Electronic-Knowledge Information Tool for Organizing Site Information and Improving Technical Communication with Stakeholders - 13082

    SciTech Connect

    Kautsky, Mark; Findlay, Richard C.; Hodges, Rex A.

    2013-07-01

    Managing technical references for projects that have long histories is hampered by the large collection of documents, each of which might contain discrete pieces of information relevant to the site conceptual model. A database application has been designed to improve the efficiency of retrieving technical information for a project. Although many databases are currently used for accessing analytical and geo-referenced data, applications designed specifically to manage technical reference material for projects are scarce. Retrieving site data from the array of available references becomes an increasingly inefficient use of labor. The electronic-Knowledge Information Tool (e-KIT) is designed as a project-level resource to access and communicate technical information. The e-KIT is a living tool that grows as new information becomes available, and its value to the project increases as the volume of site information increases. Having all references assembled in one location with complete reference citations and links to elements of the site conceptual model offers a way to enhance communication with outside groups. The published and unpublished references are incorporated into the e-KIT, while the compendium of references serves as a complete bibliography for the project. (authors)

  8. Record of Communication Concerning Ceiling Fan and Ceiling Fan Light Kit Framework Document—Docket No. EERE-2012-BT-STD-0045

    Energy.gov [DOE]

    This memo provides an overview of communications made to DOE staff on the subject of possible changes to standards and test procedures for ceiling fans and ceiling fan light kits.

  9. LANL12-RS-108J Report on Device Modeler Testing of the Device Modeler Tool Kit. DMTK in FY14

    SciTech Connect

    Temple, Brian Allen; Pimentel, David A.

    2014-09-28

    This document covers the various testing and modifications of the Device Modeler Tool Kit (DMTK) for project LANL12-RS-108J in FY14. The testing has been comprised of different device modelers and trainees for device modeling using DMTK on the secure network for a few test problems. Most of these problems have been synthetic data problems. There has been a local secure network training drill where one of the trainees has used DMTK for real data. DMTK has also been used on a laptop for a deployed real data training drill. Once DMTK gets into the home team, it will be used for more training drills (TDs) which will contain real data in the future.

  10. RM12-2703 Advanced Rooftop Unit Control Retrofit Kit Field Demonstration: Hawaii and Guam Energy Improvement Technology Demonstration Project

    SciTech Connect

    Doebber, I.; Dean, J.; Dominick, J.; Holland, G.

    2014-03-01

    As part of its overall strategy to meet its energy goals, the Naval Facilities Engineering Command (NAVFAC) partnered with U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) to rapidly demonstrate and deploy cost-effective renewable energy and energy efficiency technologies. This was one of several demonstrations of new and underutilized commercial energy efficiency technologies. The consistent year-round demand for air conditioning and dehumidification in Hawaii provides an advantageous demonstration location for advanced rooftop control (ARC) retrofit kits to packaged rooftop units (RTUs). This report summarizes the field demonstration of ARCs installed on nine RTUs serving a 70,000-ft2 exchange store (large retail) and two RTUs, each serving small office buildings located on Joint Base Pearl Harbor-Hickam (JBPHH).

  11. Effects of military-authorized activities on the San Joaquin kit fox (Vulpes velox macrotis) at Camp Roberts Army National Guard Training Site, California

    SciTech Connect

    Berry, W.H.; Standley, W.G.; O`Farrell, T.P.; Kato, T.T.

    1992-10-01

    The effects of military-authorized activities on San Joaquin kit fox (Vulpes velox macrotis) were investigated at Camp Roberts Army National Guard Training Site from 1988 to 1991. Military-authorized activities included military training exercises, facilities maintenance, new construction, controlled burning, livestock grazing, and public-access hunting. Positive effects of the military included habitat preservation, preactivity surveys, and natural resources management practices designed to conserve kit foxes and their habitat. Perceived negative effects such as entrapment in dens, shootings during military exercises, and accidental poisoning were not observed. Foxes were observed in areas being used simultaneously by military units. Authorized activities were known to have caused the deaths of three of 52 radiocollared foxes recovered dead: one became entangled in concertina wire, one was believed shot by a hunter, and one was struck by a vehicle. Entanglement in communication wire may have contributed to the death of another radiocollared fox that was killed by a predator. Approximately 10% of kit fox dens encountered showed evidence of vehicle traffic, but denning sites did not appear to be a limiting factor for kit foxes.

  12. SU-E-T-350: Verification of Gating Performance of a New Elekta Gating Solution: Response Kit and Catalyst System

    SciTech Connect

    Xie, X; Cao, D; Housley, D; Mehta, V; Shepard, D

    2014-06-01

    Purpose: In this work, we have tested the performance of new respiratory gating solutions for Elekta linacs. These solutions include the Response gating and the C-RAD Catalyst surface mapping system.Verification measurements have been performed for a series of clinical cases. We also examined the beam on latency of the system and its impact on delivery efficiency. Methods: To verify the benefits of tighter gating windows, a Quasar Respiratory Motion Platform was used. Its vertical-motion plate acted as a respiration surrogate and was tracked by the Catalyst system to generate gating signals. A MatriXX ion-chamber array was mounted on its longitudinal-moving platform. Clinical plans are delivered to a stationary and moving Matrix array at 100%, 50% and 30% gating windows and gamma scores were calculated comparing moving delivery results to the stationary result. It is important to note that as one moves to tighter gating windows, the delivery efficiency will be impacted by the linac's beam-on latency. Using a specialized software package, we generated beam-on signals of lengths of 1000ms, 600ms, 450ms, 400ms, 350ms and 300ms. As the gating windows get tighter, one can expect to reach a point where the dose rate will fall to nearly zero, indicating that the gating window is close to beam-on latency. A clinically useful gating window needs to be significantly longer than the latency for the linac. Results: As expected, the use of tighter gating windows improved delivery accuracy. However, a lower limit of the gating window, largely defined by linac beam-on latency, exists at around 300ms. Conclusion: The Response gating kit, combined with the C-RAD Catalyst, provides an effective solution for respiratorygated treatment delivery. Careful patient selection, gating window design, even visual/audio coaching may be necessary to ensure both delivery quality and efficiency. This research project is funded by Elekta.

  13. Nucleic acid detection kits

    DOEpatents

    Hall, Jeff G.; Lyamichev, Victor I.; Mast, Andrea L.; Brow, Mary Ann; Kwiatkowski, Robert W.; Vavra, Stephanie H.

    2005-03-29

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.

  14. Teacher Resource Kit

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    in which there is magnetic force). The facility is called the National High Magnetic Field Laboratory. You are welcome to share a tour of the facility by visiting the virtual...

  15. Solar Kit Lessons

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    ... 1. Lamp and solar cell positioned as described ... morning. (Daily data on solar altitude vs. time of day for specified ... such hydrocarbons as coal, petroleum, and natural gas. ...

  16. 2014-10-27 Issuance: Energy Conservation Standards for Ceiling Fan Light Kits; Notice of Public Meeting and Availability of the Preliminary Technical Support Document

    Office of Energy Efficiency and Renewable Energy (EERE)

    This document is a pre-publication Federal Register notice of public meeting and availability of the preliminary technical support document regarding energy conservation standards for ceiling fan light kits, as issued by the Deputy Assistant Secretary for Energy Efficiency on October 27, 2014. Though it is not intended or expected, should any discrepancy occur between the document posted here and the document published in the Federal Register, the Federal Register publication controls. This document is being made available through the Internet solely as a means to facilitate the public's access to this document.

  17. Establishment of a novel high-affinity IgE receptor-positive canine mast cell line with wild-type c-kit receptors

    SciTech Connect

    Amagai, Yosuke; Tanaka, Akane; Ohmori, Keitaro; Matsuda, Hiroshi

    2008-02-15

    Much is known regarding participations of mast cells with innate and acquired immunity by secreting various cytokines and chemical mediators. However, details of mast cell biology still remain unclear. In this study, we successfully established a novel growth factor-independent mast cell line (MPT-1) derived from canine mast cell tumor. MPT-1 cells manifested factor-independent proliferation as floating cells containing a large amount of histamine, as well as chymase-like dog mast cell protease 3, in cytosolic granules. Particularly, MPT-1 cells expressed high-affinity IgE receptors (Fc{epsilon}RI) and wild-type c-kit receptors. Degranulation of MPT-1 cells was induced not only by stimulation with calcium ionophore but also by cross-linkage of the surface IgE. Given that MPT-1 is the first mast cell line with Fc{epsilon}RI which has no c-kit mutations, MPT-1 cells may provide great contribution for investigation of IgE-mediated activation mechanisms of mast cells, leading to development of effective treatment for allergic disorders.

  18. Oncoprotein protein kinase antibody kit

    DOEpatents

    Karin, Michael; Hibi, Masahiko; Lin, Anning

    2008-12-23

    An isolated polypeptide (JNK) characterized by having a molecular weight of 46 kD as determined by reducing SDS-PAGE, having serine and threonine kinase activity, phosphorylating the c-Jun N-terminal activation domain and polynucleotide sequences and method of detection of JNK are provided herein. JNK phosphorylates c-Jun N-terminal activation domain which affects gene expression from AP-1 sites.

  19. Model Analysis ToolKit

    Energy Science and Technology Software Center

    2015-05-15

    MATK provides basic functionality to facilitate model analysis within the Python computational environment. Model analysis setup within MATK includes: - define parameters - define observations - define model (python function) - define samplesets (sets of parameter combinations) Currently supported functionality includes: - forward model runs - Latin-Hypercube sampling of parameters - multi-dimensional parameter studies - parallel execution of parameter samples - model calibration using internal Levenberg-Marquardt algorithm - model calibration using lmfit package - modelmore » calibration using levmar package - Markov Chain Monte Carlo using pymc package MATK facilitates model analysis using: - scipy - calibration (scipy.optimize) - rpy2 - Python interface to R« less

  20. Media Kit | Department of Energy

    Energy.gov [DOE] (indexed site)

    Media Resources Press Release: "Energy Dept. Announces New Prize Challenge to Drive Down the Cost of Solar Power," May 20, 2014 Press Release: "Energy Dept. Announces Projects to ...

  1. Northern New Mexico Citizens' Advisory Board Meeting

    Office of Environmental Management (EM)

    November 15, 2016 1:00 p.m. to 5:15 p.m. El Monte Sagrado Rio Grande Ballroom 317 Kit Carson Road Taos, New Mexico 87571 AGENDA Time Action Presenter 1:00 p.m. Call to Order Lee Bishop, DDFO Welcome and Introductions Gerard Martínez y Valencia, Chair Welcome to Taos Councilor Darien Fernandez Approval of Agenda Approval of Minutes of September 28, 2016 Approval of Minutes of October 26, 2016 1:15 p.m. Old Business a. Report from Chair Gerard Martinez y Valencia, Chair b. Other items 1:30 p.m.

  2. Developing an Energy Performance Modeling Startup Kit

    SciTech Connect

    none,

    2012-10-01

    In 2011, the NAHB Research Center began assessing the needs and motivations of residential remodelers regarding energy performance remodeling. This report outlines: the current remodeling industry and the role of energy efficiency; gaps and barriers to adding energy efficiency into remodeling; and support needs of professional remodelers to increase sales and projects involving improving home energy efficiency.

  3. Developing an Energy Performance Modeling Startup Kit

    SciTech Connect

    Wood, A.

    2012-10-01

    In 2011, the NAHB Research Center began the first part of the multi-year effort by assessing the needs and motivations of residential remodelers regarding energy performance remodeling. The scope is multifaceted - all perspectives will be sought related to remodeling firms ranging in size from small-scale, sole proprietor to national. This will allow the Research Center to gain a deeper understanding of the remodeling and energy retrofit business and the needs of contractors when offering energy upgrade services. To determine the gaps and the motivation for energy performance remodeling, the NAHB Research Center conducted (1) an initial series of focus groups with remodelers at the 2011 International Builders' Show, (2) a second series of focus groups with remodelers at the NAHB Research Center in conjunction with the NAHB Spring Board meeting in DC, and (3) quantitative market research with remodelers based on the findings from the focus groups. The goal was threefold, to: Understand the current remodeling industry and the role of energy efficiency; Identify the gaps and barriers to adding energy efficiency into remodeling; and Quantify and prioritize the support needs of professional remodelers to increase sales and projects involving improving home energy efficiency. This report outlines all three of these tasks with remodelers.

  4. Land Ice Verification and Validation Kit

    SciTech Connect

    2015-07-15

    To address a pressing need to better understand the behavior and complex interaction of ice sheets within the global Earth system, significant development of continental-scale, dynamical ice-sheet models is underway. The associated verification and validation process of these models is being coordinated through a new, robust, python-based extensible software package, the Land Ice Verification and Validation toolkit (LIVV). This release provides robust and automated verification and a performance evaluation on LCF platforms. The performance V&V involves a comprehensive comparison of model performance relative to expected behavior on a given computing platform. LIVV operates on a set of benchmark and test data, and provides comparisons for a suite of community prioritized tests, including configuration and parameter variations, bit-4-bit evaluation, and plots of tests where differences occur.

  5. Land Ice Verification and Validation Kit

    Energy Science and Technology Software Center

    2015-07-15

    To address a pressing need to better understand the behavior and complex interaction of ice sheets within the global Earth system, significant development of continental-scale, dynamical ice-sheet models is underway. The associated verification and validation process of these models is being coordinated through a new, robust, python-based extensible software package, the Land Ice Verification and Validation toolkit (LIVV). This release provides robust and automated verification and a performance evaluation on LCF platforms. The performance V&Vmore » involves a comprehensive comparison of model performance relative to expected behavior on a given computing platform. LIVV operates on a set of benchmark and test data, and provides comparisons for a suite of community prioritized tests, including configuration and parameter variations, bit-4-bit evaluation, and plots of tests where differences occur.« less

  6. Spot test kit for explosives detection

    DOEpatents

    Pagoria, Philip F; Whipple, Richard E; Nunes, Peter J; Eckels, Joel Del; Reynolds, John G; Miles, Robin R; Chiarappa-Zucca, Marina L

    2014-03-11

    An explosion tester system comprising a body, a lateral flow membrane swab unit adapted to be removeably connected to the body, a first explosives detecting reagent, a first reagent holder and dispenser operatively connected to the body, the first reagent holder and dispenser containing the first explosives detecting reagent and positioned to deliver the first explosives detecting reagent to the lateral flow membrane swab unit when the lateral flow membrane swab unit is connected to the body, a second explosives detecting reagent, and a second reagent holder and dispenser operatively connected to the body, the second reagent holder and dispenser containing the second explosives detecting reagent and positioned to deliver the second explosives detecting reagent to the lateral flow membrane swab unit when the lateral flow membrane swab unit is connected to the body.

  7. tsk_get_files - "The Sleuth Kit"

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    is accidentally removed, either by an admin or a user. Licensing Status: Available for Express Licensing (?). This software is open source. To download, please visit github...

  8. Probe kit for identifying a base in a nucleic acid

    DOEpatents

    Fodor, Stephen P. A.; Lipshutz, Robert J.; Huang, Xiaohua

    2001-01-01

    Devices and techniques for hybridization of nucleic acids and for determining the sequence of nucleic acids. Arrays of nucleic acids are formed by techniques, preferably high resolution, light-directed techniques. Positions of hybridization of a target nucleic acid are determined by, e.g., epifluorescence microscopy. Devices and techniques are proposed to determine the sequence of a target nucleic acid more efficiently and more quickly through such synthesis and detection techniques.

  9. MeSh ToolKit v1.2

    Energy Science and Technology Software Center

    2004-05-15

    MSTK or Mesh Toolkit is a mesh framework that allows users to represent, manipulate and query unstructured 3D arbitrary topology meshes in a general manner without the need to code their own data structures. MSTK is a flexible framework in that is allows (or will eventually allow) a wide variety of underlying representations for the mesh while maintaining a common interface. It will allow users to choose from different mesh representations either at initialization ormore » during the program execution so that the optimal data structures are used for the particular algorithm. The interaction of users and applications with MSTK is through a functional interface that acts as through the mesh always contains vertices, edges, faces and regions and maintains connectivity between all these entities.« less

  10. Chemical analysis kit for the presence of explosives

    DOEpatents

    Eckels, Joel Del; Nunes; Peter J.; Alcaraz, Armando; Whipple, Richard E.

    2011-05-10

    A tester for testing for explosives associated with a test location comprising a first explosives detecting reagent; a first reagent holder, the first reagent holder containing the first explosives detecting reagent; a second explosives detecting reagent; a second reagent holder, the second reagent holder containing the second explosives detecting reagent; a sample collection unit for exposure to the test location, exposure to the first explosives detecting reagent, and exposure to the second explosives detecting reagent; and a body unit containing a heater for heating the sample collection unit for testing the test location for the explosives.

  11. Improving productivity with a CCC tool kit. [Utility macros

    SciTech Connect

    Cort, G.

    1987-01-01

    A typical utility macro tool set is described. Included are SIMPORT and SEXPORT that provide syntax-directed import and export, EDIT-HOST which permits CCC resident texts to be edited with a host editor, INITIATE-LOGIN which automatically executes a hierarchy of user- and manager-defined login texts, and BATCH whicn permits CCC macros to be executed in batch mode from within CCC.

  12. Ceiling Fan Light Kits (CFLKs) Preliminary Analysis Notice of...

    Energy.gov [DOE] (indexed site)

    ... B. Product Price Determination As mentioned in section III.A, DOE often develops cost-effi... Chapters 2 and 6 of the preliminary TSD provide detail on the estimation of end-user ...

  13. Soil sampling kit and a method of sampling therewith

    DOEpatents

    Thompson, Cyril V.

    1991-01-01

    A soil sampling device and a sample containment device for containing a soil sample is disclosed. In addition, a method for taking a soil sample using the soil sampling device and soil sample containment device to minimize the loss of any volatile organic compounds contained in the soil sample prior to analysis is disclosed. The soil sampling device comprises two close fitting, longitudinal tubular members of suitable length, the inner tube having the outward end closed. With the inner closed tube withdrawn a selected distance, the outer tube can be inserted into the ground or other similar soft material to withdraw a sample of material for examination. The inner closed end tube controls the volume of the sample taken and also serves to eject the sample. The soil sample containment device has a sealing member which is adapted to attach to an analytical apparatus which analyzes the volatile organic compounds contained in the sample. The soil sampling device in combination with the soil sample containment device allow an operator to obtain a soil sample containing volatile organic compounds and minimizing the loss of the volatile organic compounds prior to analysis of the soil sample for the volatile organic compounds.

  14. Soil sampling kit and a method of sampling therewith

    DOEpatents

    Thompson, C.V.

    1991-02-05

    A soil sampling device and a sample containment device for containing a soil sample is disclosed. In addition, a method for taking a soil sample using the soil sampling device and soil sample containment device to minimize the loss of any volatile organic compounds contained in the soil sample prior to analysis is disclosed. The soil sampling device comprises two close fitting, longitudinal tubular members of suitable length, the inner tube having the outward end closed. With the inner closed tube withdrawn a selected distance, the outer tube can be inserted into the ground or other similar soft material to withdraw a sample of material for examination. The inner closed end tube controls the volume of the sample taken and also serves to eject the sample. The soil sample containment device has a sealing member which is adapted to attach to an analytical apparatus which analyzes the volatile organic compounds contained in the sample. The soil sampling device in combination with the soil sample containment device allows an operator to obtain a soil sample containing volatile organic compounds and minimizing the loss of the volatile organic compounds prior to analysis of the soil sample for the volatile organic compounds. 11 figures.

  15. Energy Kits & Materials | Photosynthetic Antenna Research Center

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    30, 1999 http://www.eia.doe.gov N Y M E X F u t u r e P r i c e s v s H e n r y H u b S p o t P r i c e s 0 . 5 0 0 . 7 5 1 . 0 0 1 . 2 5 1 . 5 0 1 . 7 5 2 . 0 0 2 . 2 5 2 . 5 0 2 . 7 5 3 . 0 0 3 . 2 5 Dollars Per Million BTU N Y M E X S e t t le m e n t P r ic e H e n r y H u b S p o t N o t e : T h e H e n r y H u b s p o t p r i c e i s f r o m t h e G A S D A I L Y a n d i s t h e m id p o i n t o f t h e i r h ig h a n d l o w p r i c e f o r a d a y . T h e d a t e s 0 . 0 0 M O N T H N Y

  16. Model Analysis ToolKit (MATK), Version 0

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Version 0 MATK provides basic functionality to facilitate model analysis within the Python computational environment. June 19, 2015 software MATK provides basic functionality to...

  17. Household energy use in urban Venezuela: Implications from surveys in Maracaibo, Valencia, Merida, and Barcelona-Puerto La Cruz

    SciTech Connect

    Figueroa, M.J.; Sathaye, J.

    1993-08-01

    This report identifies the most important results of a comparative analysis of household commercial energy use in Venezuelan urban cities. The use of modern fuels is widespread among all cities. Cooking consumes the largest share of urban household energy use. The survey documents no use of biomass and a negligible use of kerosene for cooking. LPG, natural gas, and kerosene are the main fuels available. LPG is the fuel choice of low-income households in all cities except Maracaibo, where 40% of all households use natural gas. Electricity consumption in Venezuela`s urban households is remarkably high compared with the levels used in households in comparable Latin American countries and in households of industrialized nations which confront harsher climatic conditions and, therefore, use electricity for water and space heating. The penetration of appliances in Venezuela`s urban households is very high. The appliances available on the market are inefficient, and there are inefficient patterns of energy use among the population. Climate conditions and the urban built form all play important roles in determining the high level of energy consumption in Venezuelan urban households. It is important to acknowledge the opportunities for introducing energy efficiency and conservation in Venezuela`s residential sector, particularly given current economic and financial constraints, which may hamper the future provision of energy services.

  18. Alineasolar Alternas SA | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Alternas SA Jump to: navigation, search Name: Alineasolar Alternas SA Place: Valencia, Spain Zip: 46021 Product: Valencia-based designer, seller and installer of turnkey...

  19. Iberdrola Renovables | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Renovables Jump to: navigation, search Name: Iberdrola Renovables Place: Valencia, Spain Zip: 46023 Sector: Renewable Energy Product: Valencia-based renewable energy project...

  20. Photon emission in neutral current interactions with nucleons...

    Office of Scientific and Technical Information (OSTI)

    de Fsica Terica, Centro Mixto Universidad de Valencia-CSIC, E-46071 Valencia (Spain) Publication Date: 2015-05-15 OSTI Identifier: 22391806 Resource Type: Journal Article...

  1. Photo-z optimization for measurements of the BAO radial scale...

    Office of Scientific and Technical Information (OSTI)

    i Estudis Avancats, Barcelona (Spain) Instituto de Fisica Corpuscular (CSIC-UVEG), Valencia (Spain) Instituto de Fisica Corpuscular (CSIC-UVEG), Valencia (Spain) Publication ...

  2. CX-002655: Categorical Exclusion Determination

    Energy.gov [DOE]

    Valencia County, New Mexico Date: 05/28/2010Location(s): Valencia County, New MexicoOffice(s): Energy Efficiency and Renewable Energy

  3. Southwestern Power Administration

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Carlos Valencia Named Director of Transmission Engineering and Planning Carlos Valencia Named Director of Division of Transmission Engineering and Planning Carlos Valencia Carlos Valencia has been selected to serve as Director of the Division of Transmission Engineering and Planning at Southwestern Power Administration (Southwestern), effective June 29, 2014. Valencia, who has served as the agency's Director of Maintenance since 2009, has extensive experience in the management of transmission

  4. Energy Conservation Standards for Ceiling Fan Light Kits Notice of Proposed Rulemaking

    Energy.gov [DOE]

    Energy Conservation Program: Energy Conservation Standards for Ceiling Fan Notice of proposed rulemaking (NOPR) and announcement of public meeting.

  5. Design Analysis Kit for Optimization and Terascale Applications 6.0

    SciTech Connect

    2015-10-19

    Sandia's Dakota software (available at http://dakota.sandia.gov) supports science and engineering transformation through advanced exploration of simulations. Specifically it manages and analyzes ensembles of simulations to provide broader and deeper perspective for analysts and decision makers. This enables them to: (1) enhance understanding of risk, (2) improve products, and (3) assess simulation credibility. In its simplest mode, Dakota can automate typical parameter variation studies through a generic interface to a computational model. However, Dakota also delivers advanced parametric analysis techniques enabling design exploration, optimization, model calibration, risk analysis, and quantification of margins and uncertainty with such models. It directly supports verification and validation activities. The algorithms implemented in Dakota aim to address challenges in performing these analyses with complex science and engineering models from desktop to high performance computers.

  6. U-119: Blackberry PlayBook Unspecified WebKit Bug Lets Remote...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    7.1, and BlackBerry PlayBook tablet software ABSTRACT: A remote user can create HTML that, when loaded by the target user, will execute arbitrary code on the target user's...

  7. RM12-2703 Advanced Rooftop Unit Control Retrofit Kit Field Demonstrati...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    ... ARC systems use wireless communication from RTU to RTU and then cellular communication ... Some utilities employ a peak demand charge in addition to charging for energy consumption. ...

  8. Kits and methods of detection using cellulose binding domain fusion proteins

    DOEpatents

    Shoseyov, O.; Yosef, K.

    1998-04-14

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 16 figs.

  9. Kits and methods of detection using cellulose binding domain fusion proteins

    DOEpatents

    Shoseyov, Oded

    1998-01-01

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  10. T-579: BlackBerry Device Software Bug in WebKit Lets Remote Users...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    or instant messages. BlackBerry has described a workaround (disabling the use of JavaScript in the BlackBerry Browser) in their advisory. BlackBerry Device storage space...

  11. LANL12-RS-108J Device Modeler Tool Kit - DMTK Final Report for...

    Office of Scientific and Technical Information (OSTI)

    Country of Publication: United States Language: English Subject: Mathematics & Computing(97) Word Cloud More Like This Full Text File size NAView Full Text View Full Text DOI: ...

  12. Design Analysis Kit for Optimization and Terascale Applications 6.0

    Energy Science and Technology Software Center

    2015-10-19

    Sandia's Dakota software (available at http://dakota.sandia.gov) supports science and engineering transformation through advanced exploration of simulations. Specifically it manages and analyzes ensembles of simulations to provide broader and deeper perspective for analysts and decision makers. This enables them to: (1) enhance understanding of risk, (2) improve products, and (3) assess simulation credibility. In its simplest mode, Dakota can automate typical parameter variation studies through a generic interface to a computational model. However, Dakota also deliversmore » advanced parametric analysis techniques enabling design exploration, optimization, model calibration, risk analysis, and quantification of margins and uncertainty with such models. It directly supports verification and validation activities. The algorithms implemented in Dakota aim to address challenges in performing these analyses with complex science and engineering models from desktop to high performance computers.« less

  13. Evaluation of aftermarket CNG conversion kits in light-duty vehicle applications. Final report

    SciTech Connect

    Blazek, C.F.; Rowley, P.F.; Grimes, J.W.

    1995-07-01

    The Institute of Gas Technology (IGT) was contracted by the National Renewable Energy Laboratory (NREL) to evaluate three compressed natural gas (CNG) conversion systems using a 1993 Chevrolet Lumina baseline vehicle. A fourth conversion system was added to the test matrix through funding support from Brooklyn Union. The objective of this project was to measure the Federal Test Procedure (FTP) emissions and fuel economy of the different conversion systems, and to compare the performance to gasoline-fueled operation and each other. Different natural gas compositions were selected to represent the 10th percentile, mean, and 90th percentile compositions distributed in the Continental United States. Testing with these different compositions demonstrated the systems` ability to accommodate the spectrum of gas found in the United States. Each compressed natural gas conversion system was installed and adjusted according to the manufacturer`s instructions. In addition to the FTP testing, an evaluation of the comparative installation times and derivability tests (based on AGA and CRC guidelines) were conducted on each system.

  14. Boosted objects and jet substructure at the LHC: Report of BOOST2012, held at IFIC Valencia, 23rd-27th of July 2012

    SciTech Connect

    Altheimer, A.

    2014-03-21

    This report of the BOOST2012 workshop presents the results of four working groups that studied key aspects of jet substructure. We discuss the potential of first-principle QCD calculations to yield a precise description of the substructure of jets and study the accuracy of state-of-the-art Monte Carlo tools. Limitations of the experiments ability to resolve substructure are evaluated, with a focus on the impact of additional (pile-up) proton proton collisions on jet substructure performance in future LHC operating scenarios. The final section summarizes the lessons learnt from jet substructure analyses in searches for new physics in the production of boosted top quarks.

  15. Boosted objects and jet substructure at the LHC: Report of BOOST2012, held at IFIC Valencia, 23rd-27th of July 2012

    DOE PAGES [OSTI]

    Altheimer, A.

    2014-03-21

    This report of the BOOST2012 workshop presents the results of four working groups that studied key aspects of jet substructure. We discuss the potential of first-principle QCD calculations to yield a precise description of the substructure of jets and study the accuracy of state-of-the-art Monte Carlo tools. Limitations of the experiments’ ability to resolve substructure are evaluated, with a focus on the impact of additional (pile-up) proton proton collisions on jet substructure performance in future LHC operating scenarios. The final section summarizes the lessons learnt from jet substructure analyses in searches for new physics in the production of boosted topmore » quarks.« less

  16. IM2 Energia Solar | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    IM2 Energia Solar Jump to: navigation, search Name: IM2 Energia Solar Place: Valencia, Spain Zip: 46002 Sector: Solar Product: Valencia-based developer of roof-mounted solar...

  17. PR B_WY_C BM HILIGHT POWELL KIT TY WELL D RAW SC OT T MIKES D

    Energy Information Administration (EIA) (indexed site)

    Liquids Reserve Class No 2001 liquids reserves 0.1 - 10 Mbbl 10.1 - 100 Mbbl 100.1 - 1,000 Mbbl 1,000.1 - 10,000 Mbbl 10,000.1 - 100,000 Mbbl Basin Outline CO Index Map For 2 ...

  18. PR B_WY_C BM HILIGHT POWELL KIT TY WELL D RAW SC OT T MIKES D

    Energy Information Administration (EIA) (indexed site)

    Gas Reserve Class No 2001 gas reserves 0.1 - 10 MMCF 10.1 - 100 MMCF 100.1 - 1,000 MMCF 1,000.1 - 10,000 MMCF 10,000.1 - 100,000 MMCF > 100,000 MMCF Basin Outline CO Index Map For ...

  19. PR B_WY_C BM HILIGHT POWELL KIT TY WELL D RAW SC OT T MIKES D

    Energy Information Administration (EIA) (indexed site)

    BOE Reserve Class No 2001 reserves 0.1 - 10 MBOE 10.1 - 100 MBOE 100.1 - 1,000 MBOE 1,000.1 - 10,000 MBOE 10,000.1 - 100,000 MBOE > 100,000 MBOE Basin Outline CO Index Map For 2 Powder River Basin Panels WY MT SD NE ND Powder River Basin 1 2 NE Total Total Total Number Liquid Gas BOE of Reserves Reserves Reserves Fields (Mbbl) (MMcf) (Mbbl) Powder River 543 193,456 2,398,604 593,223 Basin 2001 Reserve Summary for All Powder River Basin Fields PR B_WY_C BM PR B_WY_C BM PR B_WY_C BM PR B_WY_C

  20. PR B_WY_C BM HILIGHT POWELL KIT TY WELL D RAW SC OT T MIKES D

    Energy Information Administration (EIA) (indexed site)

    Gas Reserve Class No 2001 gas reserves 0.1 - 10 MMCF 10.1 - 100 MMCF 100.1 - 1,000 MMCF 1,000.1 - 10,000 MMCF 10,000.1 - 100,000 MMCF > 100,000 MMCF Basin Outline CO Index Map For 2 Powder River Basin Panels WY MT SD NE ND Powder River Basin 1 2 NE Total Total Total Number Liquid Gas BOE of Reserves Reserves Reserves Fields (Mbbl) (MMcf) (Mbbl) Powder River 543 193,456 2,398,604 593,223 Basin 2001 Reserve Summary for All Powder River Basin Fields PR B_WY_C BM PR B_WY_C BM PR B_WY_C BM PR

  1. U-119: Blackberry PlayBook Unspecified WebKit Bug Lets Remote Users Execute Arbitrary Code

    Energy.gov [DOE]

    A remote user can create HTML that, when loaded by the target user, will execute arbitrary code on the target user's system

  2. PR B_WY_C BM HILIGHT POWELL KIT TY WELL D RAW SC OT T MIKES D

    Gasoline and Diesel Fuel Update

    Office of Oil and Gas, Energy Information Administration pursuant to studies required by Section 604 of the Energy Policy and Conservation Act Amendments of 2000 (P.L. 106-469). ...

  3. PR B_WY_C BM HILIGHT POWELL KIT TY WELL D RAW SC OT T MIKES D

    Energy Information Administration (EIA) (indexed site)

    Authors: Sam Limerick (1), Lucy Luo (1), Gary Long (2), David Morehouse (2), Jack Perrin (1), Steve Jackson (1) and Robert King (2) (1) Z, Inc., (2) Energy Information ...

  4. Environmental planning and categorical exclusions: Making the categorical exclusion an integral part of your NEPA tool kit

    SciTech Connect

    Holthoff, M.G.; Hanrahan, T.P.

    1994-06-01

    As contained in the Regulations for Implementing the Procedural Provisions of the National Environmental Policy Act, 40 CFR 1500--1508, the Council on Environmental Quality (CEQ) directs federal agencies to adopt their own procedures for implementing the Act. The US Department of Energy (DOE) and the US Department of Agriculture Forest Service (USFS) are two examples of federal agencies with dissimilar but functionally equivalent CX processes. The DOE and USFS were selected as subjects for this study because of their distinctly different missions and as a results of the author`s familiarity with the policies of both agencies. The objectives of this study are to: (1) describe the CX policies and processes of the two agencies, (2) identify the similarities and differences between the two processes, and (3) suggest ways for improving these processes. In performing this evaluation, the authors will identify the components of each agency`s CX process that clearly contributes qualitative information for the purpose of making environmental planning decisions. Drawing from the best elements of each process, the authors will provide some general recommendations that should enable the agencies to fulfill their various obligations to the CX process while concurrently performing early, thorough, and expeditious environmental reviews under NEPA.

  5. PNC Bank Equipment Finance and Energy Group | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    to: navigation, search Name: PNC Bank - Equipment Finance and Energy Group Place: Valencia, California Zip: 91355 Product: Energy and Equipment Finance arm of PNC Bank...

  6. SOLAR MODELS WITH ACCRETION. I. APPLICATION TO THE SOLAR ABUNDANCE...

    Office of Scientific and Technical Information (OSTI)

    7300, University of California, Berkeley, CA 94720-7300 (United States) Instituto de Fisica Corpuscular (CSIC-UVEG), Aptdo. 22085, E-46071 Valencia (Spain) Publication Date: ...

  7. Energias Renovables Mediterraneas RENOMAR | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Mediterraneas RENOMAR Jump to: navigation, search Name: Energias Renovables Mediterraneas (RENOMAR) Place: Valencia, Spain Zip: 46002 Sector: Wind energy Product: Wind project...

  8. Microsoft PowerPoint - 130424_Sandia_TEL_V3_1_compressed.pptx

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    ... Valencia 2010 J. Sutterlueti et al.; "Detailed Outdoor Performance Analysis of Thin Film and Cristalline Silicon Based Reference PV Power Plants in Switzerland"; 3 rd Int. ...

  9. Sand2004-0522

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    2004 DESIGN STUDIES FOR TWIST-COUPLED WIND TURBINE BLADES James Locke and Ulyses Valencia ... efficiency increase, so do the challenges of developing innovative design solutions. ...

  10. Enerstar SA | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Enerstar SA Jump to: navigation, search Name: Enerstar SA Place: Gandia, Valencia, Spain Zip: 467022 Sector: Solar Product: Developing a 50.0MW parabolic trough solar thermal...

  11. SERSA | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    SERSA Jump to: navigation, search Name: SERSA Place: Valencia, Spain Zip: 46010 Sector: Wind energy Product: Project developer, primarily in the wind sector. Coordinates:...

  12. Technosun | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Technosun Jump to: navigation, search Name: Technosun Place: Spain Sector: Solar Product: Solar products distributor based in Valencia, Spain. References: Technosun1 This article...

  13. --No Title--

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    C. Valencia, Barry Lai, Guofeng Zhang, Jill K. Paterson, Francois Rouzaud, Werner Berens, Stephen M. Wincovitch, Susan H. Garfield, Richard D. Leapman, Vincent J. Hearing, Michael...

  14. Phase development in conventional and active belite cement pastes...

    Office of Scientific and Technical Information (OSTI)

    Departamento de Quimica Inorganica, Universidad de Malaga, 29071 Malaga (Spain) European ... Benjamin Franklin, 17 Paterna, Valencia (Spain) Departamento de Quimica Inorganica, ...

  15. SOLAR 97 CONFERENCE: MANUSCRIPT PREPARATION INSTRUCTIONS

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    ... Model for PV Modules of Any Available Technology: Proc. of the 25th European Photovoltaic Solar Energy Conference, Valencia, Spain, September 2010 (4) Maxwell, E. L., A ...

  16. Gesfesa Energia SL | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Gesfesa Energia SL Jump to: navigation, search Name: Gesfesa Energia SL Place: Valencia, Spain Zip: 46004 Sector: Solar Product: Involved in construction of buidings and and solar...

  17. Prosolia Energia Solar | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Prosolia Energia Solar Jump to: navigation, search Name: Prosolia Energia Solar Place: Ontinyent, Valencia, Spain Product: Develops PV projects in Spain, and sells them to...

  18. Siliken | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Place: Valencia, Spain Product: Spanish manufacturer of PV modules and turnkey manufacturing equipment; it has a silicon and silane production division. Coordinates:...

  19. H2Scan LLC | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    search Name: H2Scan LLC Place: Valencia, California Zip: 91355 Sector: Hydro, Hydrogen Product: Hydrogen specific sensing systems, uniquely able to detect hydrogen against...

  20. Wind Alliance Group | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    search Name: Wind Alliance Group Place: Valencia, Spain Sector: Wind energy Product: Spanish wind developer with subsidiaries throughout emerging Europe Coordinates: 39.468791,...

  1. Ener t Global SA | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    navigation, search Name: Ener-t Global SA Place: Valencia, Spain Zip: 46870 Product: Spanish developer of parabolic trough thermal plants on a turnkey basis. Coordinates:...

  2. Husesolar SL | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    to: navigation, search Name: Husesolar SL Place: Valencia, Spain Zip: 46009 Product: A Spanish project developer. Coordinates: 39.468791, -0.376913 Show Map Loading map......

  3. Instructional Materials | Photosynthetic Antenna Research Center

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Solar Energy Learn about the quality of electromagnetic radiation produced by the sun and ... Kit 5: Making a Dye Sensitized Solar Cell Kit 6A: Efficiency of Solar Cells Kit ...

  4. IDS-NF Impact of Neutrino Cross Section Impact of Neutrino Cross Section

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    IDS-NF Impact of Neutrino Cross Section Impact of Neutrino Cross Section Knowledge on Oscillation Knowledge on Oscillation Measurements Measurements M. Sorel, IFIC (CSIC and U. of Valencia) IDS-NF, RAL, Jan 16-17 2008 M. Sorel - IFIC (Valencia U. & CSIC) 2 IDS-NF Neutrino Cross Sections: At What Energies Needed? Superbeams: Solid: T2K Dashed: NovA M. Sorel - IFIC (Valencia U. & CSIC) 3 IDS-NF Neutrino Cross Sections: At What Energies Needed? Superbeams: Solid: T2K Dashed: NovA Beta

  5. Remote Alaskan Communities Energy Efficiency Competition | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    As part of President Obama's commitment to fight climate change and assist remote Alaskan ... Media Kit For Stakeholders FACT SHEET: About RACEE DOWNLOAD: Social Media Kit ENERGY BLOG ...

  6. Water-Efficient Technology Opportunity: Steam Sterilizer Condensate...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Steam Sterilizer Condensate Retrofit Kit Water-Efficient Technology Opportunity: Steam ... sterilizer condensate retrofit kits as a water-saving technology that is relevant to the ...

  7. Remote Alaskan Communities Energy Efficiency Competition Social...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Remote Alaskan Communities Energy Efficiency Competition Social Media Kit Remote Alaskan Communities Energy Efficiency Competition Social Media Kit Alaska Stakeholders-Below you ...

  8. United Nations Environment Programme (UNEP) | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Climate Change Resource Kit UNEP Energy Resource Kit UNEP Global Trends in Sustainable Energy Investment 2009 Report UNEP Handbook for Drafting Laws on Energy Efficiency and...

  9. MiniBooNE/LSND Neutrino Oscillation Results

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    M. Sorel (IFIC - CSIC & U. Valencia) Workshop on Beyond Three Family Neutrino Oscillations May 3-4, 2011, LNGS (Italy) 1. LSND e (1993-2001) 2. MiniBooNE ...

  10. ABO Wind Espana SA | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    ABO Wind Espana SA Place: Valencia, Spain Zip: 46002 Sector: Wind energy Product: Spanish branch of project developer ABO Wind AG. Coordinates: 39.468791, -0.376913 Show...

  11. Regional math teacher wins presidential award

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Regional math teacher wins presidential award Community Connections: Your link to news and opportunities from Los Alamos National Laboratory Latest Issue:November 2, 2016 all issues All Issues » submit Regional math teacher wins presidential award Regional economic growth celebrated February 1, 2014 Vivian Valencia, (fourth-grade math teacher at the San Juan Elementary School in Espanola) recently won the Presidential Award for Excellence in Mathematics and Science Teaching Vivian Valencia,

  12. Notices Evening Scoping Meeting

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    813 Federal Register / Vol. 81, No. 195 / Friday, October 7, 2016 / Notices Evening Scoping Meeting Date: Wednesday, October 26, 2016. Time: 6:00 p.m. Location: Embassy Suites by Hilton Valencia, 28508 Westinghouse Place, Valencia, CA, 91355. Phone: (661) 257-3111. Scoping Document 1 (SD1), which outlines the subject areas to be addressed in the environmental document, was mailed to the individuals and entities on the Commission's mailing list. Copies of SD1 will be available at the scoping

  13. Original Impact Calculations

    Energy.gov [DOE]

    Original Impact Calculations, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  14. STEP Participant Survey Executive Summary

    Energy.gov [DOE]

    STEP Participant Survey Executive Summary, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  15. STEP Utility Data Release Form

    Energy.gov [DOE]

    STEP Utility Data Release Form, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  16. Maryland Efficiency Program Options

    Office of Energy Efficiency and Renewable Energy (EERE)

    Maryland Efficiency Program Options, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  17. Protocol for House Parties

    Energy.gov [DOE]

    Protocol for House Parties, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  18. Video Shoot Scope of Work

    Energy.gov [DOE]

    Video Shoot Scope of Work, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  19. Program Launch Letter from Mayor

    Energy.gov [DOE]

    Program Launch Letter from Mayor, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  20. STEP Intern Job Description

    Office of Energy Efficiency and Renewable Energy (EERE)

    STEP Intern Job Description, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  1. Advisory Committee Minutes

    Office of Energy Efficiency and Renewable Energy (EERE)

    Advisory Committee Minutes, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  2. STEP Request For Incentives

    Office of Energy Efficiency and Renewable Energy (EERE)

    STEP Request For Incentives, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  3. Partner Letter of Engagement

    Energy.gov [DOE]

    Partner Letter of Engagement, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  4. Evaluation Consultant RFP

    Office of Energy Efficiency and Renewable Energy (EERE)

    Evaluation Consultant RFP, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  5. STEP Intern Reference Check Sheet

    Energy.gov [DOE]

    STEP Intern Reference Check Sheet, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  6. STEP Participant Financing Options

    Energy.gov [DOE]

    STEP Participant Financing Options, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  7. STEP Partner Presentation

    Energy.gov [DOE]

    STEP Partner Presentation, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  8. STEP Program Benchmark Report

    Energy.gov [DOE]

    STEP Program Benchmark Report, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  9. Communications Consultant RFP

    Energy.gov [DOE]

    Communications Consultant RFP, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  10. Communication Consultant Contract

    Energy.gov [DOE]

    Communication Consultant Contract, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  11. STEP Participant Survey Report

    Office of Energy Efficiency and Renewable Energy (EERE)

    STEP Participant Survey Report, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  12. Statement of Project Objectives

    Office of Energy Efficiency and Renewable Energy (EERE)

    Statement of Project Objectives, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  13. STEP Conference Presentation

    Energy.gov [DOE]

    STEP Conference Presentation, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  14. STEP Utility Bill Analysis Report

    Energy.gov [DOE]

    STEP Utility Bill Analysis Report, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  15. Advisory Committee Agenda

    Energy.gov [DOE]

    Advisory Committee Agenda, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  16. Quarterly Progress Report

    Energy.gov [DOE]

    Quarterly Progress Report, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  17. STEP Participation Agreement

    Energy.gov [DOE]

    STEP Participation Agreement, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  18. STEP Preferred Contractors

    Energy.gov [DOE]

    STEP Preferred Contractors, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  19. Solar Cookers International | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Solar Cookers International Jump to: navigation, search CooKit Each group of items costs 75 Kenya Shillings (about US1) as does the pile of charcoal shown. By using a CooKit or...

  20. Jefferson Lab - News Media

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    790 en Media Kit https:www.jlab.orgmedia-kit

  1. Sol Inc | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    15 lighting kits to Port-au-Prince. Each lighting kit contains a pole, an LED lamp, a solar panel and a battery. About half the lights were allocated to a medical clinic and...

  2. DAKOTA 6.0

    Energy Science and Technology Software Center

    001217MLTPL03 Design Analysis Kit for Optimization and Terascale Applications 6.0 http://dakota.sandia.gov

  3. DAKOTA 5.0

    Energy Science and Technology Software Center

    001217MLTPL02 DAKOTA Design Analysis Kit for Optimization and Terascale https://www.cs.sandia.gov/dakota/documentation.html

  4. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov [DOE] (indexed site)

    kits. A... Eligibility: Commercial, Industrial Savings Category: Clothes Washers, Dishwasher, RefrigeratorsFreezers, Dehumidifiers, Lighting, Lighting ControlsSensors, Other...

  5. Recombinant hydrogen-producing cyanobacterium and uses thereof

    DOEpatents

    Xu, Qing; Smith, Hamilton O

    2014-10-14

    A recombinant cyanobacterium comprising an oxygen-tolerant, hydrogen-evolving hydrogenase, kit, and methods of use.

  6. Early leukemia diagnostics using microsphere arrays

    DOEpatents

    Nolan, John P.; Zhou, Feng

    2007-02-20

    The present invention provides methods and kits for detecting chromosome translocations. The present invention further provides methods for diagnosing cancer.

  7. STEP Non-Participant Survey Report

    Energy.gov [DOE]

    STEP Non-Participant Survey Report, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  8. Letters of Outreach to Partner Communities

    Energy.gov [DOE]

    Letters of Outreach to Partner Communities, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  9. STEP-by-STEP Program Summary

    Energy.gov [DOE]

    STEP-by-STEP Program Summary, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  10. STEP Energy Coach and Technical Consultant Job Descriptions

    Energy.gov [DOE]

    STEP Energy Coach and Technical Consultant Job Descriptions, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  11. Water-Efficient Technology Opportunity: Steam Sterilizer Condensate

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Retrofit Kit | Department of Energy Steam Sterilizer Condensate Retrofit Kit Water-Efficient Technology Opportunity: Steam Sterilizer Condensate Retrofit Kit Steam sterilizers are heated by steam that condenses and flows to the trap drain beneath the sterilizer. Steam sterilizers are heated by steam that condenses and flows to the trap drain beneath the sterilizer. The Federal Energy Management Program (FEMP) identified steam sterilizer condensate retrofit kits as a water-saving technology

  12. SHORT-BASELINE NEUTRINO PHYSICS AT FERMILAB WESLEY KETCHUM LOS ALAMOS NATIONAL LABORATORY

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    NEUTRINO PHYSICS AT FERMILAB WESLEY KETCHUM LOS ALAMOS NATIONAL LABORATORY 7/4/14 ICHEP 2014, Valencia, Spain 1 LIQUID ARGON TPCS ON THE BOOSTER NEUTRINO BEAMLINE TO EXPLORE NEW PHYSICS Phys. Rev. D79, 072002 (2009) THE BOOSTER NEUTRINO BEAMLINE (BNB) * 8 GeV protons hit Be target * Magnetic horn focuses (defocuses) neutrino (antineutrino) parents * Muon neutrino energy peaks ~700 MeV * Previous experiments on BNB: MiniBooNE & SciBooNE 7/4/14 ICHEP 2014, Valencia, Spain 2 Detector(s)

  13. SuperB Progress Report: Detector

    SciTech Connect

    Grauges, E.; Donvito, G.; Spinoso, V.; Manghisoni, M.; Re, V.; Traversi, G.; Eigen, G.; Fehlker, D.; Helleve, L.; Carbone, A.; Di Sipio, R.; Gabrielli, A.; Galli, D.; Giorgi, F.; Marconi, U.; Perazzini, S.; Sbarra, C.; Vagnoni, V.; Valentinetti, S.; Villa, M.; Zoccoli, A.; /INFN, Bologna /Bologna U. /Caltech /Carleton U. /Cincinnati U. /INFN, CNAF /INFN, Ferrara /Ferrara U. /UC, Irvine /Taras Shevchenko U. /Orsay, LAL /LBL, Berkeley /UC, Berkeley /Frascati /INFN, Legnaro /Orsay, IPN /Maryland U. /McGill U. /INFN, Milan /Milan U. /INFN, Naples /Naples U. /Novosibirsk, IYF /INFN, Padua /Padua U. /INFN, Pavia /Pavia U. /INFN, Perugia /Perugia U. /INFN, Perugia /Caltech /INFN, Pisa /Pisa U. /Pisa, Scuola Normale Superiore /PNL, Richland /Queen Mary, U. of London /Rutherford /INFN, Rome /Rome U. /INFN, Rome2 /Rome U.,Tor Vergata /INFN, Rome3 /Rome III U. /SLAC /Tel Aviv U. /INFN, Turin /Turin U. /INFN, Padua /Trento U. /INFN, Trieste /Trieste U. /TRIUMF /British Columbia U. /Montreal U. /Victoria U.

    2012-02-14

    This report describes the present status of the detector design for SuperB. It is one of four separate progress reports that, taken collectively, describe progress made on the SuperB Project since the publication of the SuperB Conceptual Design Report in 2007 and the Proceedings of SuperB Workshop VI in Valencia in 2008.

  14. Stereolithography models. Final report

    SciTech Connect

    Smith, R.E.

    1995-03-01

    This report describes the first stereolithographic models made, which proved in a new release of ProEngineer software (Parametric Technologies, or PTC) and 3D Systems (Valencia, California) software for the SLA 250 machine. They are a model of benzene and the {alpha}-carbon backbone of the variable region of an antibody.

  15. In other news

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    In other news Community Connections: Your link to news and opportunities from Los Alamos National Laboratory Latest Issue:November 2, 2016 all issues All Issues » submit In other news Connections has a new title, winners of the Robot Rodeo, local firm gets large grant and new kits available for regional teachers July 1, 2013 A new STEM teacher kit is being distributed throughout the region A new STEM teacher kit is being distributed throughout the region Contact Editor Linda Anderman Email

  16. CASL - Public Release of CASL Infrastructure Software

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Public Release of CASL Infrastructure Software The Virtual Environment for Reactor Applications (VERA) comprises a suite of tools for scalable simulation of nuclear reactor core behavior. The following three key components of the VERA infrastructure have been released and made publicly-available: Data TransferKit Data TransferKit (DTK) is a software package designed to provide grid transfer services to the various CASL physics components. The Data Transfer Kit has been redesigned to provide a

  17. Technical Consultant Report Template | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Technical Consultant Report Template Technical Consultant Report Template Technical Consultant Report Template, from the Tool Kit Framework: Small Town University Energy Program ...

  18. Dr

    Energy Saver

    ... (air source) 2005 Ceiling fans and ceiling fan light kits 2005 Low voltage dry-type distribution transformers 2005 Unit heaters 2005 Commercial prerinse spray valves 2005 ...

  19. DOE Issues Final Appliance Test Procedure Rule | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    fans and ceiling fan light kits; illuminated exit signs; torchieres; low-voltage dry-type distribution transformers; traffic signal modules and pedestrian modules; unit heaters; ...

  20. STEP Financial Incentives Summary | Department of Energy

    Energy Saver

    STEP Financial Incentives Summary STEP Financial Incentives Summary STEP Financial Incentives Summary, from the Tool Kit Framework: Small Town University Energy Program (STEP). PDF ...

  1. Astatinated organic compounds

    DOEpatents

    Milius, Richard A.; Lambrecht, Richard M.; Bloomer, William D.

    1989-05-02

    Methods and kits for incorporating a radioactive astatine isotope (particularly .sup.211 At) into an organic compound by electrophilic astatodestannylation of organostannanes.

  2. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov [DOE] (indexed site)

    Plant (CMLP) offers rebates to commercial customers for a variety of appliances, ETS heating systems, general lighting upgrades, CFL bulbs, and exit sign retrofit kits. A......

  3. EERE Success Story-Colorado: Energy Modeling Products Support...

    Energy.gov [DOE] (indexed site)

    utility that supplies electricity and natural gas to eight ... energy consumption, and OpenStudio(tm), a software development kit that simplifies the creation of end-user ...

  4. Xcel Energy - Residential Energy Efficiency Rebate Program |...

    Energy.gov [DOE] (indexed site)

    ResidentialSolutionsRebates... Expiration Date 12312014 State New Mexico Program Type Rebate Program Rebate Amount Draft Check Kits and Power Check Meters:...

  5. Ethanol production in non-recombinant hosts

    DOEpatents

    Kim, Youngnyun; Shanmugam, Keelnatham; Ingram, Lonnie O.

    2013-06-18

    Non-recombinant bacteria that produce ethanol as the primary fermentation product, associated nucleic acids and polypeptides, methods for producing ethanol using the bacteria, and kits are disclosed.

  6. V-161: IBM Maximo Asset Management Products Java Multiple Vulnerabilities

    Energy.gov [DOE]

    Asset and Service Mgmt Products - Potential security exposure when using JavaTM based applications due to vulnerabilities in Java Software Developer Kits.

  7. V-143: Fresh Java issues being exploited in the wild

    Energy.gov [DOE]

    Java issues are being exploited in the wild by exploit kits, with Cool and Redkit specifically being known to use these bugs, and others likely to follow shortly.

  8. Outreach for Educators | Photosynthetic Antenna Research Center

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    for. Click Here to RSVP for the Hot Topics Workshops General Information Outreach for Educators Energy Kits Instructional Materials Teacher Leader Program Web Resources Hot Topics...

  9. UNCLASSIFIED Institute for Materials ...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Emeritus Institute for Theoretical Condensed Matter Physics (KIT) The Exotic World of Quantum Matter: Novel States Induced by Fluctuations Tuesday, June 30, 2015 2 - 3pm MSL...

  10. U.S. Department of Energy Categorical Exclusion Determination...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Ceiling Fan Light Kits (RIN: 1904-AC87) EERE- Buildings Technology Program Nationwide In this final rule, DOE proposes new and amended energy conservation standards for ceiling fan...

  11. U.S. Department of Energy Categorical Exclusion Determination...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Ceiling Fan Light Kits (RIN: 1904-AC87) EERE- Buildings Technology Program Nationwide In this proposed rule, DOE proposes new and amended energy conservation standards for ceiling...

  12. Enforcement Policy: Certain Ceiling Fans and Certain Ceiling...

    Office of Environmental Management (EM)

    Enforcement Policy: Certain Ceiling Fans and Certain Ceiling Fan Light Kits Issued: December 15, 2015 Revised: March 15, 2016 DOE announced an enforcement policy in a Federal ...

  13. B O N N E V I L L E P O W E R A D M I N I S T R A T I O N

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    May 2013 Commercial & Industrial Lighting LED Retrofits for Troffers LED retrofit kits and linear replacement lamps are now avail- able to convert recessed fluorescent light...

  14. Curriculum Development | Photosynthetic Antenna Research Center

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    are focused on solar energy and other renewable energy topics. The kits work to bring science content standards to life through real-world investigations & hands-on...

  15. Home Front Inc.: Hot, Humid Climate Region 40+% Energy Savings

    SciTech Connect

    2009-08-13

    This case study describes a model prototype home kit that is efficient and affordable and includes high-performance mechanical systems, HVAC, and solar water heating.

  16. Astatinated organic compounds

    DOEpatents

    Milius, R.A.; Lambrecht, R.M.; Bloomer, W.D.

    1989-05-02

    Methods and kits for incorporating a radioactive astatine isotope (particularly [sup 211]At) into an organic compound by electrophilic astatodestannylation of organostannanes. 3 figs.

  17. lep

    National Nuclear Security Administration (NNSA)

    components to address aging, ensure extended service life and improve safety, reliability and security of the bomb. With the incorporation of an Air Force tail-kit assembly,...

  18. Luma Resources LLC | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Rochester Hills, Michigan Zip: 48309 Sector: Solar Product: Michigan-based developer and installer of solar roof kits for the residential market. References: Luma Resources LLC1...

  19. conference {15169

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    ... Conformal and Non-conformal Adaptive Mesh Refinement with Hierarchical Array-based ... are meshed using MeshKit tools or the CUBIT mesh generation toolkit, optionally ...

  20. %A L. A. Bautista Gomez

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    ... %T Conformal and Non-conformal Adaptive Mesh Refinement with Hierarchical Array-based ... are meshed using MeshKit tools or the CUBIT mesh generation toolkit, optionally ...

  1. ISSUANCE 2015-12-15: Energy Conservation Program: Test Procedures for

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Ceiling Fan Light Kits, Final Rule | Department of Energy 15: Energy Conservation Program: Test Procedures for Ceiling Fan Light Kits, Final Rule ISSUANCE 2015-12-15: Energy Conservation Program: Test Procedures for Ceiling Fan Light Kits, Final Rule This document is the Energy Conservation Program: Test Procedures for Ceiling Fan Light Kits, Final Rule. cflk tp final rule 2015-12-15.pdf (817.76 KB) More Documents & Publications 2014-10-27 Issuance: Test Procedure for Ceiling Fan Light

  2. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov [DOE] (indexed site)

    and T5 linear fluorescents, screw-in LED lamps and LED trim kits and reduced wattage ceramic metal halide lamps. All incentives are provided through participating electrical...

  3. Momentum-Savings-Reports

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Residential Room Air Conditioning Residential Ceiling Fan Light Kits Electric Clothes Dryers Electric Cooktops Electric Ovens Residential Furnace Fans Microwave Ovens...

  4. EECBG Success Story: County Aims to Save with Upgrades | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Aims to Save with Upgrades EECBG Success Story: County Aims to Save with Upgrades August ... Learn more. Addthis Related Articles EECBG Success Story: Classes, Kits and Energy Savings ...

  5. 2014-10-27 Issuance: Energy Conservation Standards for Ceiling...

    Energy Saver

    Energy Conservation Standards for Ceiling Fan Light Kits; Notice of Public Meeting and Availability of the Preliminary Technical Support Document 2014-10-27 Issuance: Energy ...

  6. ISSUANCE 2015-06-25: Energy Conservation Program: Energy Conservation...

    Energy Saver

    Program: Energy Conservation Standards for Ceiling Fan Light Kits, Notice of Proposed Rulemaking ISSUANCE 2015-06-25: Energy Conservation Program: Energy Conservation Standards for ...

  7. ISSUANCE 2015-12-15: Energy Conservation Program: Test Procedures...

    Office of Environmental Management (EM)

    15: Energy Conservation Program: Test Procedures for Ceiling Fan Light Kits, Final Rule ISSUANCE 2015-12-15: Energy Conservation Program: Test Procedures for Ceiling Fan Light ...

  8. University Park “STEP-UP” Proposal

    Office of Energy Efficiency and Renewable Energy (EERE)

    University Park “STEP-UP” Proposal: DE-FOA-0000148, from the Tool Kit Framework: Small Town University Energy Program (STEP).

  9. Window Attachments

    Energy Saver

    ... shades Surface applied film Cellular shade Window quilt Seasonal film kit Louvered blinds Roller shades Solar screens Cellular shades Surface applied films Exterior attachments ...

  10. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Patent Program Document Software Manual Technical Report ... Research and Development (NA-22) Office of River ... Search for: All records CreatorsAuthors contains: "KIT, ...

  11. New Formulas for America's Workforce

    Energy Saver

    ... engineering); assembling solar cars from kits and racing ... role of an independent petroleum company with geologists ... 88 and single-sex groups vs. mixed-sex groups, 32 ...

  12. Pueblo of Laguna Utility Authority

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    PUEBLO OF LAGUNA UTILITY AUTHORITY TRIBAL UTILITY FORMATION: REGULATION, FINANCE AND BUSINESS STRUCTURE FACTS ON LAGUNA PUEBLO * LAGUNA IS LOCATED ABOUT 45 MILES WEST OF ALBUQUERQUE ON INTERSTATE 40 * RESERVATION CONSISTS OF APPROX. 500,000 ACRES OF LAND SITUATED IN CIBOLA, VALENCIA AND BERNALILLO COUNTIES * SIX (6) VILLAGES, LAGUNA, MESITA, PAGUATE, SEAMA, ENCINAL, PARAJE ARE ALL WITHIN THE LAGUNA RESERVATION * 4,000+ TRIBAL MEMBERS LIVE ON THE RESERVATION * CASINOS, TRAVEL CENTERS, SUPERMARKET

  13. Northern New Mexico Citizens' Advisory Board

    Office of Environmental Management (EM)

    , 2016 Northern New Mexico Citizens' Advisory Board 94 Cities of Gold Road, Santa Fe, New Mexico 87506 Executive Committee Roster 1. Gerard Martinez y Valencia, NNMCAB Chair 2. Angelica Gurulé, NNMCAB Vice-Chair 3. Stephen Schmelling, Environmental Monitoring & Remediation Committee Chair 4. Doug Sayre, Environmental Monitoring & Remediation Vice-Chair 5. Angelica Gurulé, Waste Management Committee Chair 6. Carlos Valdez, Waste Management Committee Vice-Chair 7. Menice Santistevan,

  14. Symposium

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Symposium Student Symposium Symposium provides a unique opportunity for students to present their research, broadening their expertise and preparing them for careers in science and nontechnical fields. Contact Technical Host Scott Robbins 505-667-3639 Email Registration/Abstract Information Selena Valencia 505-665-0905 Email Judges Brenda Montoya 505-667-4866 Email Student Symposium August 3, 2016 - UNM-LA Red Flyer of 2016 Student Symposium This year's Symposium,"Celebrating Student

  15. Solar Site Survey Toolkit

    Energy.gov [DOE]

    After a couple outings, a principal technologist at Sandia National Laboratories saw a need for a travel kit that would have the necessary tools to make the task of site surveys more manageable and safer. They have had great success using the kit in the field already.

  16. BP-12 Final Models Datasets

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    MB, XLS) Tier 2 Module of RAM RAM Tier 2 Pricing Module (4.8 MB, XLS) Tool Kit Files (TPP) ToolKit v4.8.1 BP-12 Final Studies Release 4-Aug-11 (12.5 MB, XLS) Risk Mod Output...

  17. Endangered species and cultural resources program Naval petroleum Reserves in California. Annual report FY96

    SciTech Connect

    1997-07-01

    In FY96, Enterprise Advisory Services, Inc. (EASI) continued to support efforts to protect endangered species and cultural resources at the Naval Petroleum Reserves in California (NPRC). These efforts are conducted to ensure NPRC compliance with regulations regarding the protection of listed species and cultural resources on federal properties. Population monitoring activities were conducted for San Joaquin kit foxes, giant kangaroo rats, blunt-nosed leopard lizards, and Hoover`s wooly-star. Kit fox abundance and distribution was assessed by live-trapping over a 329-km{sup 2} area. Kit fox reproduction and mortality were assessed by radiocollaring and monitoring 22 adults and two pups. Reproductive success and litter size were determined through live-trapping and den observations. Rates and sources of kit fox mortality were assessed by recovering dead radiocollared kit foxes and conducting necropsies to determine cause of death. Abundance of coyotes and bobcats, which compete with kit foxes, was determined by conducting scent station surveys. Kit fox diet was assessed through analysis of fecal samples collected from live-trapped foxes. Abundance of potential prey for kit foxes was determined by conducting transect surveys for lagornorphs and live-trapping small mammals.

  18. Private sector village enterprise a new approach to sustainable financing

    SciTech Connect

    Gay, C.F.

    1997-12-01

    This paper presents an enterprise plan for introducing solar power in a rural market, while providing economic development, and hence the ability of the user to pay for the power source. This plan is based on a product called GEEP - a solar sewing machine conversion kit. This kit can be retrofit onto pedal sewing machines and marketed to village tailors in India, as part of a marketing program which includes increased demand for tailored products which will allow the tailors to be able to finance the conversion kits.

  19. PPL Electric Utilities- Residential Energy Efficiency Rebate Program

    Energy.gov [DOE]

    Additional incentives are available for qualifying low-income residential customers. The E-power Wise program provides energy education workshops and provides low cost energy efficiency kits to...

  20. HKC-US: Order (2013-SE-33002)

    Energy.gov [DOE]

    DOE ordered HKC-US, LLC to pay a $6,500 civil penalty after finding HKC-US had failed to certify that one basic model of ceiling fan light kit complies with the applicable energy conservation standards.

  1. Ex Parte Memorandum | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Tuesday, May 4, 2010, Kit Kennedy and Kate Houren of the Natural Resources Defense Council (NRDC) initiated a telephone call with Daniel Cohen and Bryan Miller of the Department of ...

  2. Energy Department Releases Updates to EnergyPlus and OpenStudio...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    OpenStudio 1.7.0 OpenStudio is DOE's open-source software development kit for whole-building energy modeling using EnergyPlus and daylight analysis using Radiance. DOE releases ...

  3. Article: OpenStudio Measures

    Energy.gov [DOE]

    This article discusses a scripting facility called Measures for the OpenStudio software development kit. The article was published in the journal Energy and Buildings in September 2015.

  4. Ethanologenic bacteria with increased resistance to furfural

    DOEpatents

    Miller, Elliot Norman; Jarboe, Laura R.; Yomano, Lorraine P.; York, Sean W.; Shanmugam, Keelnatham; Ingram, Lonnie O'Neal

    2015-10-06

    The invention relates to bacterium that have increased resistance to furfural and methods of preparation. The invention also relates to methods of producing ethanol using the bacterium and corresponding kits.

  5. Low Income Workgroup Meeting Notes Portland, Oregon

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Tri-cities, so the utility runs workshop ads out right after that first big bill. Has a prop house and Todd installs the entire kit. Funds come from public information budget...

  6. Diagnostics on Demand | GE Global Research

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    The "Diagnostics on Demand" Infectious Disease Test Kit Click to email this to a friend (Opens in new window) Share on Facebook (Opens in new window) Click to share (Opens in new ...

  7. LANAI HIGH-DENSITY IRRADIANCE SENSOR NETWORK FOR CHARACTERIZING...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    ... cellular based 900MHz types and other IEEE 802.11 wireless local area network solutions. ... The outdoor enclosure and solar-charging kit used in this application were obtained from ...

  8. HKC-US: Proposed Penalty (2013-SE-33002)

    Office of Energy Efficiency and Renewable Energy (EERE)

    DOE alleged in a Notice of Proposed Civil Penalty that HKC-US, LLC failed to certify a ceiling fan light kit as compliant with the applicable energy conservation standards.

  9. Policy Flash 2012-61 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    1 Policy Flash 2012-61 Attached is Policy Flash 2012-61 COR Tool Kit Rescission. Questions concerning this policy flash should be directed to Lorri Wilkins of the Professional...

  10. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... air showers Belov, K. ; UCLA Caltech, JPL ; Mulrey, K. ; Delaware U. ; Romero-Wolf, A. ; Caltech, JPL ; Wissel, S.A. ; UCLA ; Zilles, A. ; KIT, Karlsruhe, EKP ; et al ...

  11. CenterPoint Energy's Energy Wise Program

    Energy.gov [DOE]

    CenterPoint Energy's Energy Wise program provides take-home kits containing efficiency devices with classroom and in-home education techniques with the aim to inspire families to adopt new resource...

  12. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    "Hawaii U.UCLAHawaii U.Delaware U.University Coll., LondonChicago U.Stanford U., Phys. Dept.KIT, Karlsruhe, EKP" Name Name ORCID Search Authors Type: All BookMonograph ...

  13. Living Comfortably: A Consumer's Guide to Home Energy Upgrades...

    Energy Saver

    ... In addition to the blower door, certified energy auditors use tools -- such as gas leak detectors, carbon monoxide detectors, kill-a-watt meters and lead-safe testing kits -- to ...

  14. CX-100270 Categorical Exclusion Determination | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Determination Proposed Rulemaking for Energy Conservation Standards for Ceiling Fan Light Kits RIN: 1904-AC87 CX(s) Applied: B5.1 EERE- Buildings Technology Program Date:...

  15. Mr. John Kiel

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    ... (1) First Aid Kit (24) per station; includes, (3) Band-Aid Combo Paks (2) Swabs, PVP (1) Antibiotic Ointment (1) Sting-Kill Swab (2) Dressing, compresses (2) Roller Bandages ...

  16. Mr. John E. Kieling, Chief Hazardous Waste Bureau

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    ... (1) First Aid Kit (24) per station; includes, (3) Band-Aid Combo Paks (2) Swabs, PVP (1) Antibiotic Ointment (1) Sting-Kill Swab (2) Dressing, compresses (2) Roller Bandages ...

  17. Royal Pacific: Proposed Penalty (2013-SE-33004)

    Energy.gov [DOE]

    DOE alleged in a Notice of Proposed Civil Penalty that Royal Pacific, Ltd. failed to certify ceiling fans, ceiling fan light kits, medium base compact fluorescent lamps, and illuminated exit signs as compliant with the applicable energy conservation standards.

  18. Flexitron | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Solar Product: Manufactures and distributes own-brand PV-powered lamps and a solar electric bicycle conversion kit. Coordinates: 12.97092, 77.60482 Show Map Loading...

  19. EDrive Systems LLC | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    EDrive Systems LLC Place: Los Angeles, California Product: Developer of a plug-in and battery kit for the Toyota Prius. References: EDrive Systems LLC1 This article is a stub....

  20. Tax Credits, Rebates & Savings | Department of Energy

    Energy.gov [DOE] (indexed site)

    to rewire the fixture. Eligible lamps include screw-in CFLs, reduced wattage T8 and T5 linear fluorescents, screw-in LED lamps and LED trim kits and reduced wattage ceramic...

  1. Microsoft Word - EIA_7A_USER_GUIDE_FINAL_4.23.15

    Gasoline and Diesel Fuel Update

    ... This application is a web-based front end for the survey Form EIA-7A and provides an ... Kit 1.5. It resides on a web application server in the EIA Network Infrastructure. ...

  2. Toxicology evaluation and hazard review for non-CFC containing rigid foams BKC 44317 and last-a-foam MSL-02A

    SciTech Connect

    Greulich, K.A.; Archuleta, M.M.

    1996-06-01

    New pour-in-place, low density, rigid polyurethane foam kits have been developed to mechanically stabilize damaged explosive ordnance. Although earlier foam systems used chlorofluorocarbons as blowing agents, the current versions rely on carbon dioxide generated by the reaction of isocynates with water. In addition, these kits were developed to manually generate small quantifies of rigid foam in the field with minimal or no protective equipment. The purpose of this study was to evaluate and summarize available hazard information for the components of these rigid foam kits and to provide recommendations for personal protective equipment to be used while performing the manual combination of the components. As with most rigid foam systems, these kits consist of two parts, one a mixture of isocyanates; the other, a combination of polyols, surfactants, and amine catalysts. Once completely deployed, the rigid foam is non-toxic. The components, however, have some important health effects which must be considered when establishing handling procedures.

  3. Model Car Race, Building Competition Illuminate Solar Energy...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Students in the Solar Model Building Competition use a K'NEX Energy System Kit Model, which contains 1,082 pieces, two motors and two solar panels. With it, they must design and ...

  4. FY06 AR

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    ... The novel fi eld kit employs a heated diode sensor (commonly used to fi nd refrigerant leaks) to detect halogenated volatile organic compounds in soil and water at a sensitivity ...

  5. Record of Communication Concerning Ceiling Fan and Ceiling Fan...

    Office of Environmental Management (EM)

    Record of Communication Concerning Ceiling Fan and Ceiling Fan Light Kit Framework Document-Docket No. EERE-2012-BT-STD-0045 Record of Communication Concerning Ceiling Fan and ...

  6. CX-005575: Categorical Exclusion Determination

    Energy.gov [DOE]

    Meter Test Kit Development for Accuracy TestingCX(s) Applied: B3.6Date: 04/07/2011Location(s): Bristol, PennsylvaniaOffice(s): Energy Efficiency and Renewable Energy, National Energy Technology Laboratory

  7. Microsoft Word - MPW_Fact_Sheet_SAND2010-4820P_updated_format...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Inte rvices integra afer resource circuits in low rogram prov production fa test and pac ened, 3.3 v, 0 ors, and diod tal and analo n Kit. managed and oper artment of Energy r...

  8. Microsoft Word - Document25

    Office of Environmental Management (EM)

    that no lead is present); however, agencies should exercise caution since not all spot-test kits are useful as a negative screen. The following considerations are offered as a...

  9. Hospital Sterile Processing | GE Global Research

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    accounting and sterilization of surgical tools and kits. You Might Also Like IMG0475 Innovation 247: We're Always Open MunichinteriorV 10 Years ON: From the Lab to the...

  10. NNSA Marks Two-Year Construction Milestone at MOX Facility in South

    National Nuclear Security Administration (NNSA)

    Carolina | National Nuclear Security Administration | (NNSA) Marks Two-Year Construction Milestone at MOX Facility in South Carolina July 31, 2009 WASHINGTON, D.C. - The National Nuclear Security Administration (NNSA) today marked the second full year of successful construction of the Mixed Oxide (MOX) Fuel Fabrication Facility at the Savannah River Site (SRS) near Aiken, South Carolina, by launching a new online multimedia kit on NNSA's website. The kit, which includes a photo gallery that

  11. Requested information regarding remote analytical capabilities

    SciTech Connect

    Steel, R.T.

    1991-09-01

    This document describes a new method for analysis of cyanide in radioactive waste samples from the Hanford Site. The new assay is designed to increase throughput of samples, reduce waste volumes and decrease radiation exposure to analysts. The system is based on the Lachat Micro-Dist{sup TM} microdistillation system. This document contains the reference and methods manual for this kit, and also the specific PNL lab procedures for using this kit. 6 refs., 20 figs. (MHB)

  12. BioReactor

    Energy Science and Technology Software Center

    2003-04-18

    BioReactor is a simulation tool kit for modeling networks of coupled chemical processes (or similar productions rules). The tool kit is implemented in C++ and has the following functionality: 1. Monte Carlo discrete event simulator 2. Solvers for ordinary differential equations 3. Genetic algorithm optimization routines for reverse engineering of models using either Monte Carlo or ODE representation )i.e., 1 or 2)

  13. Method to directly radiolabel antibodies for diagnostic imaging and therapy

    DOEpatents

    Thakur, Mathew L.

    1994-01-01

    The invention is a novel method and kit for directly radiolabeling proteins such as antibodies or antibody fragments for diagnostic and therapeutic purposes. The method comprises incubating a protein-containing solution with a solution of sodium ascorbate; adding a required quantity of reduced radionuclide to the incubated protein. A kit is also provided wherein the protein and/or reducing agents may be in lyophilized form.

  14. Method to directly radiolabel antibodies for diagnostic imaging and therapy

    DOEpatents

    Thakur, Mathew L.

    1991-01-01

    The invention is a novel method and kit for directly radiolabeling proteins such as antibodies or antibody fragments for diagnostic and therapeutic purposes. The method comprises incubating a protein-containing solution with a solution of sodium ascorbate; adding a required quantity of reduced radionuclide to the incubated protein. A kit is also provided wherein the protein and/or reducing agents may be in lyophilized form.

  15. Geralyn "Sam" Zeller

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Conferences Upcoming Conferences: Link to upcoming neutrino meetings NuInt09 - 6th Int'l Workshop on Neutrino-Nucleus Interactions in the Few GeV Region (May 18-22, 2009, Sitges, Barcelona, Spain) Past Conferences: Elba X - Electron-Nucleus Scattering Workshop (June 23-27, 2008, Marciana Marina, Elba, Italy) NuFact08 - 10th Int'l Workshop on Neutrino Factories, Superbeams, and Betabeams (June 30-July 5, 2008, Valencia, Spain) Neutrino 08 - XXIII Int'l Conference on Neutrino Physics and

  16. untitled

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Elaine Webb Project Manager Bearskin Services Tulsa, Oklahoma Special thanks to: SWPA Ron Beck Marshall Boyken Kenny Broadaway Linda Dunham Steve Hill Ricky Jones Darlene Low Harry Mardirosian Jim McDonald Ernie Millsap Beth Nielsen Margaret Skidmore Tracey Stewart Carlos Valencia CNI/Bearskin Ashley Butler Vicki Clarke Ruben Garcia William Hiller Kathy O'Neal SW Division Corps Sherman Jones Vicksburg Dist. Corps Brian Westfall Dusty Wilson U P DAT E S O U T H W E S T E R N P O W E R A D M I N I

  17. Northern New Mexico Citizens' Advisory Board

    Office of Environmental Management (EM)

    Waste Management Committee Roster 1. Angelica Gurulé, NNMCAB, Committee Chair 2. Carlos Valdez, NNMCAB, Committee Vice-Chair 3. Alex Puglisi, NNMCAB 4. Angel Quintana, NNMCAB 5. Daniel Mayfield, NNMCAB 6. Gerard Martinez y Valencia, NNMCAB 7. Irene Tse-Pe, NNMCAB 8. Joey Tiano, NNMCAB 9. Max Baca, NNMCAB 10. Mona Varela, NNMCAB 11. Tessa Jo Mascareñas, NNMCAB 12. M. Lee Bishop, DOE Co-Deputy Designated Federal Officer (CDDFO) 13. Michael Gardipe, DOE Co-Deputy Designated Federal Officer

  18. 123.indd

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Third Street Tulsa, Oklahoma 74103-3502 918-595-6600 Fax 918-595-6656 www.swpa.gov The UPDATE is published by and for customers, retirees, and employees of Southwestern Power Administration like: Kay Jones Accounting Operations Clerk Tulsa Special thanks to: Mike Deihl Ruben Garcia William Hiller Laura Holman Beth Nielsen George Robbins Tracey Stewart Cathy Stillson Gary Swartzlander Carlos Valencia Aleta Wallace Rutha Williams & Mike Carver, Fort Worth District COE U P D AT E S O U T H W E

  19. Hunter DOE Ex Parte Communication Summary July 30 2015 | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Energy DOE Ex Parte Communication Summary July 30 2015 Hunter DOE Ex Parte Communication Summary July 30 2015 This memo provides a summary of the communications made to the DOE staff regarding proposed rules on ceiling fan test procedures as well as proposed energy efficiency rules for ceiling fan light kits. July 30 2015 Hunter DOE Ex Parte Meeting (118.22 KB) More Documents & Publications ISSUANCE 2015-12-15: Energy Conservation Program: Test Procedures for Ceiling Fan Light Kits,

  20. Active and realistic passive marijuana exposure tested by three immunoassays and GC/MS in urine

    SciTech Connect

    Mule, S.J.; Lomax, P.; Gross, S.J.

    1988-05-01

    Human urine samples obtained before and after active and passive exposure to marijuana were analyzed by immune kits (Roche, Amersham, and Syva) and gas chromatography/mass spectrometry (GC/MS). Seven of eight subjects were positive for the entire five-day test period with one immune kit. The latter correlated with GC/MS in 98% of the samples. Passive inhalation experiments under conditions likely to reflect realistic exposure resulted consistently in less than 10 ng/mL of cannabinoids. The 10-100-ng/mL cannabinoid concentration range essential for detection of occasional and moderate marijuana users is thus unaffected by realistic passive inhalation.

  1. Microsoft Word - Enforcement Policy Statement - CF CFLK (05012016).docx

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Certain ceiling fans and certain ceiling fan light kits Enforcement Policy Statement: Certain ceiling fans and certain ceiling fan light kits Issued: December 15, 2015 Revised: March 15, 2016 In a final rule published on December 24, 2015, DOE explained it interprets the definition of ceiling fan to include "hugger fans" and fans capable of producing large volumes of airflow 1 (collectively, for the purposes of this policy, "subject fans"), which means that those fans are

  2. Microsoft Word - CMOS7 PDK.docx

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    CM The CM device m are need National The PDK  Phys - C v  Stan - H m P  Sc Si  CA Vi  SP an Pa  PC  Ph Ta LV OS7 P MOS7 Proces models, phys ded to design l Laboratorie K consists of sical Design Contains the verification. ndard Cell an Holds inform make up bas Physical chematic ilvaco Ga ADENCE iews PICE Mod nd SPECT assive De CELL Lay hysical V argeted T VS/DRC A DEV Tran Res Dio Cap Proce s Design Kit sical layouts, n analog or m es 0.35um S f the followi Kit resources n

  3. Use of Cost Estimating Relationships

    Directives, Delegations, and Other Requirements [Office of Management (MA)]

    1997-03-28

    Cost Estimating Relationships (CERs) are an important tool in an estimator's kit, and in many cases, they are the only tool. Thus, it is important to understand their limitations and characteristics. This chapter discusses considerations of which the estimator must be aware so the Cost Estimating Relationships can be properly used.

  4. Toolkit Model for SN-03 Final Proposal (ratecases/sn03)

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Model SN CRAC ToolKit Model Variable, Flat SN CRAC, 80% TPP (TK187SN-03FS3BPA-PropVariableFlatSNN24-Jun-03.xls, 3.1 MB) Data Input Files (required to run the above...

  5. CX-100423 Categorical Exclusion Determination

    Energy.gov [DOE]

    Final Rulemaking for Energy Conservation Standards for Ceiling Fan Lights Kits RIN 1094-AC87 CX(s) Applied: B5.1 EERE - Buildings Technology Program Date: 12/18/2015 Location(s): Nationwide Office(s): Golden Field Office

  6. Loan Loss Reserve Agreement | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Loan Loss Reserve Agreement Loan Loss Reserve Agreement Loan Loss Reserve Agreement, from the Tool Kit Framework: Small Town University Energy Program (STEP). B2 Loan Loss Reserve Agreement.pdf (59.68 KB) More Documents & Publications Energy Efficiency Loan Program Agreement Template Energy Efficiency Loan Program Agreement-Template Energy Efficiency Loan Program Agreement Template

  7. DAKOTA JAGUAR 2.1 user's Manual.

    SciTech Connect

    Adams, Brian M.; Lefantzi, Sophia; Chan, Ethan; Ruthruff, Joseph R.

    2011-06-01

    JAGUAR (JAva GUi for Applied Research) is a Java software tool providing an advanced text editor and graphical user interface (GUI) to manipulate DAKOTA (Design Analysis Kit for Optimization and Terascale Applications) input specifications. This document focuses on the features necessary for a user to use JAGUAR.

  8. DAKOTA JAGUAR 3.0 user's manual.

    SciTech Connect

    Adams, Brian M.; Bauman, Lara E; Chan, Ethan; Lefantzi, Sophia; Ruthruff, Joseph R.

    2013-05-01

    JAGUAR (JAva GUi for Applied Research) is a Java software tool providing an advanced text editor and graphical user interface (GUI) to manipulate DAKOTA (Design Analysis Kit for Optimization and Terascale Applications) input specifications. This document focuses on the features necessary to use JAGUAR.

  9. Reduced weight decontamination formulation for neutralization of chemical and biological warfare agents

    DOEpatents

    Tucker, Mark D.

    2014-06-03

    A reduced weight DF-200 decontamination formulation that is stable under high temperature storage conditions. The formulation can be pre-packed as an all-dry (i.e., no water) or nearly-dry (i.e., minimal water) three-part kit, with make-up water (the fourth part) being added later in the field at the point of use.

  10. Reduce completion fluid costs with on-site brine tests

    SciTech Connect

    Thomas, D.C.; Darlington, R.K.; Kinney, W.R.; Lowell, J.L.

    1982-09-01

    A newly developed field kit makes on-site brine completion fluid testing practical. Simple titration procedures are used to analyze brine for calcium, zinc, chloride and bromide with an accuracy and repeatability that compares favorably with expensive laboratory techniques. This article describes the field testing theory and details analytical procedures used.

  11. Royal Pacific: Order (2013-SE-33004)

    Energy.gov [DOE]

    DOE ordered Royal Pacific, Ltd. to pay a $8,000 civil penalty after finding Royal Pacific had failed to certify that various basic models of medium base compact fluorescent lamps, ceiling fans, ceiling fan light kits, and illuminated exit signs comply with the applicable energy conservation standards.

  12. JAGUAR developer's manual.

    SciTech Connect

    Chan, Ethan

    2011-06-01

    JAGUAR (JAva GUi for Applied Research) is a Java software tool providing an advanced text editor and graphical user interface (GUI) to manipulate DAKOTA (Design Analysis Kit for Optimization and Terascale Applications) input specifications. This document focuses on the technical background necessary for a developer to understand JAGUAR.

  13. Peptide and protein biomarkers for type 1 diabetes mellitus

    DOEpatents

    Zhang, Qibin; Metz, Thomas O.

    2014-06-10

    A method for identifying persons with increased risk of developing type 1 diabetes mellitus, or having type I diabetes mellitus, utilizing selected biomarkers described herein either alone or in combination. The present disclosure allows for broad based, reliable, screening of large population bases. Also provided are arrays and kits that can be used to perform such methods.

  14. Erythropoietin binding protein from mammalian serum

    DOEpatents

    Clemons, Gisela K.

    1997-01-01

    Purified mammalian erythropoietin binding-protein is disclosed, and its isolation, identification, characterization, purification, and immunoassay are described. The erythropoietin binding protein can be used for regulation of erythropoiesis by regulating levels and half-life of erythropoietin. A diagnostic kit for determination of level of erythropoietin binding protein is also described.

  15. Erythropoietin binding protein from mammalian serum

    DOEpatents

    Clemons, G.K.

    1997-04-29

    Purified mammalian erythropoietin binding-protein is disclosed, and its isolation, identification, characterization, purification, and immunoassay are described. The erythropoietin binding protein can be used for regulation of erythropoiesis by regulating levels and half-life of erythropoietin. A diagnostic kit for determination of level of erythropoietin binding protein is also described. 11 figs.

  16. New method for the selective labeling of erythrocytes in whole blood with Tc-99m

    DOEpatents

    Srivastava, S.C.; Babich, J.W.; Straub, R.; Richards, P.

    1984-01-27

    Method and kit are described for the preparation of /sup 99m/Tc labeled red blood cells using whole blood in a closed sterile system containing stannous tin in a form such that it will enter the red blood cells and be available therein for the reduction of technetium.

  17. Technical Consultant Report Template | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Technical Consultant Report Template Technical Consultant Report Template Technical Consultant Report Template, from the Tool Kit Framework: Small Town University Energy Program (STEP). C3d Technical Consultant Report Template.pdf (28.19 KB) More Documents & Publications How To Build a Wind Turbine in Less Than 20 Minutes Technical Consultant Contract Evaluation Consultant RFP

  18. STEP Brochure (Spanish) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Brochure (Spanish) STEP Brochure (Spanish) STEP Brochure (Spanish), from the Tool Kit Framework: Small Town University Energy Program (STEP). F1b STEP Brochure (Spanish).pdf (318.33 KB) More Documents & Publications Ahorre Energía STEP Brochure (Spanish) Conocimiento de Energia Get Current: Switch on Clean Energy Activity Book

  19. STEP Financial Incentives Summary | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Financial Incentives Summary STEP Financial Incentives Summary STEP Financial Incentives Summary, from the Tool Kit Framework: Small Town University Energy Program (STEP). E4 STEP Financial Incentives Summary.pdf (234.59 KB) More Documents & Publications STEP Request For Incentives Maryland Efficiency Program Options STEP Participant Financing Options

  20. Technical Consultant Contract | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Technical Consultant Contract Technical Consultant Contract Technical Consultant Contract, from the Tool Kit Framework: Small Town University Energy Program (STEP). C3c Technical Consultant Contract.pdf (202.69 KB) More Documents & Publications Communication Consultant Contract Communications Consultant RFP Loan Loss Reserve Agreement

  1. Report of endangered species studies on Naval Petroleum Reserve No. 2, Kern County, California

    SciTech Connect

    O'Farrell, T.P.; Warrick, G.D.; Mathews, N.E.; Kato, T.T.

    1987-09-01

    Between 1983 and 1986 the size of the population of San Joaquin kit foxes (Vulpes macrotis mutica) on Naval Petroleum Reserve No. 2 (NPR-2), Kern County, California, was estimated semiannually using capture-recapture techniques. Although summer population estimates varied between 222 in 1983 and 121 in 1986, and winter estimates varies between 258 in 1984 and 91 in 1983, the population appeared to remain relatively stable at an apparent norm of 165. Kit foxes were abundant even in the intensely developed areas, and numbers and densities (1.12 to 2.49/sq mile) were consistently higher on NPR-2 than on neighboring NPR-1. The percentage of adult vixens that successfully raised pups was 55%, average litter size was 4.0 +- 0.0, and the sex ratio (M:F) of 25 pups was 1:1.5. Most (45.2%) foxes were killed by coyotes (Canis latrans), vehicles killed 6.4%, and 6.5% died of other causes. A cause could not be determined for 41.9% of the deaths. There was a general increase in coyote visitation rates at scent stations, but kit fox visitation rates generally decreased. Kit fox indices were consistently higher on NPR-2 than on NPR-1. Approximately 15% of the kit foxes on NPR-2 dispersed an average of 2.2 +- 0.2 miles. Average dispersal distance did not differ between the sexes. The longest dispersal was 6.9 miles. Proportionately more male than female pups dispersed. Remains of lagomorphs (jackrabbits and cottontails) and kangaroo rats had the highest frequency of occurrence in scats. Frequency of occurrence of lagomorph remains was greater in developed than in undeveloped habitats. Proportions of lagomorph remains increased and kangaroo rat remains decreased between 1983 and 1984. 62 refs., 9 figs., 24 tabs.

  2. Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores

    DOEpatents

    Glazer, Alexander N.; Benson, Scott C.

    1998-01-01

    Novel fluorescent heterodimeric DNA-staining energy transfer dyes are provided combining asymmetric cyanine azole-indolenine dyes, which provide for strong DNA affinity, large Stokes shifts and emission in the red region of the spectrum. The dyes find particular application in gel electrophoresis and for labels which may be bound to a variety of compositions in a variety of contexts. Kits and individual compounds are provided, where the kits find use for simultaneous detection of a variety of moieties, particularly using a single narrow wavelength irradiation source. The individual compounds are characterized by high donor quenching and high affinity to dsDNA as a result of optimizing the length of the linking group separating the two chromophores.

  3. SHARP/PRONGHORN Interoperability: Mesh Generation

    SciTech Connect

    Avery Bingham; Javier Ortensi

    2012-09-01

    Progress toward collaboration between the SHARP and MOOSE computational frameworks has been demonstrated through sharing of mesh generation and ensuring mesh compatibility of both tools with MeshKit. MeshKit was used to build a three-dimensional, full-core very high temperature reactor (VHTR) reactor geometry with 120-degree symmetry, which was used to solve a neutron diffusion critical eigenvalue problem in PRONGHORN. PRONGHORN is an application of MOOSE that is capable of solving coupled neutron diffusion, heat conduction, and homogenized flow problems. The results were compared to a solution found on a 120-degree, reflected, three-dimensional VHTR mesh geometry generated by PRONGHORN. The ability to exchange compatible mesh geometries between the two codes is instrumental for future collaboration and interoperability. The results were found to be in good agreement between the two meshes, thus demonstrating the compatibility of the SHARP and MOOSE frameworks. This outcome makes future collaboration possible.

  4. Solar Circuitry

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Solar Circuitry" with the Solar Powered Energy Kit Curriculum: Solar Power- (light/electromagnetic radiation, electricity, circuitry, efficiency, energy transformation, subatomic particles) Grade Level: Middle or High School Size: Small groups, depending on ability level. Time: 4 to 5 class periods Summary: Students will learn how the solar cell changes light energy to electrical energy. Students will work in small groups and construct different solar panel configurations to see the

  5. NREL: Workforce Development and Education Programs - Middle School

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Middle School Learn about fun and exciting middle school programs and competitions that will put student's science and math skills to the test. National Middle School Science Bowl NREL Model Car Competitions Kit and Component Supplier List Printable Version NREL's Workforce Development & Education Programs Home About Workforce Development & Education Programs Grade 4-12 Students Middle School National Middle School Science Bowl NREL Model Car Competitions High School Science

  6. Guides for Freedom of Information Act and Privacy Act 1974

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Guides Guides Guides for Freedom of Information Act and Privacy Act 1974 The United States Department of Justice FOIA Guide FOIA Kit 1988 Edition of "Using the FOIA" for individuals or organizations who wish to make an FOIA application to a federal agency, containing 5 files; FOIA Instructions, FOIA Application; FOIA Fee Waiver; FOIA Appeal; selected Federal FOIA Addresses; and FBI Offices nationwide.

  7. Boise State University: Business Plan

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Vento: Wind Turbine Education Kits Business Plan Portfolio Team Members and Contacts The number of people involved in Vento 26 students with 16 mechanical engineering students, five electrical engineering students, and five business students. Our faculty and community support includes seven teachers and prominent community business owners. In the fall semester the mechanical engineering teams focused on building four different prototypes of turbines to compete while the business team focused on

  8. Boise State University: Technical Design Report

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    VENTO: Wind Power Generator Design Portfolio Boise State University Spring 2014 Michael Sansom 1 , Luke Weaver 1 , Cody McConkey 1 , Calvin Brown 1 , Stephen Stauts 2 , Brian Dambi 2 , Adrian Reyes 2 , Haitian Xu 2 , Firas Almasyabi 2 , John Gardner, Ph.D. 1 , Sarah Haight 1 , Eric Booth 2 , Matthew Dolan 2 1 Department of Mechanical and Biomedical Engineering 2 Department of Electrical and Computer Engineering Vento: Wind Turbine Education Kits Business Plan Portfolio Team Members and Contacts

  9. Building America FY14 Project Matrix - by Research Team

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    ARBI (DEG) Ducts in Condioned Space & High R-value Wall Measure Research New All Thermal Enclosure All ARBI (DEG) Field Trial of an Aerosol-Based Envelope Sealing Technology on a Production Scale Measure Research Both All Thermal Enclosure All All All All All ARBI (DEG) Reduced Recirculation Losses in Multi-Family Solar Water Heating Applications Measure Research Both MF Efficient Water Heating All ARBI (DEG) Evaluation of HPWH Ducting Kit Benefits in a Hot- Dry Climate Measure Research Both

  10. Building America FY14 Projects by Building Type

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Ducts in Condioned Space & High R-value Wall Measure Research New All Thermal Enclosure All ARBI (DEG) Field Trial of an Aerosol-Based Envelope Sealing Technology on a Production Scale Measure Research Both All Thermal Enclosure All All All All All ARBI (DEG) Evaluation of HPWH Ducting Kit Benefits in a Hot- Dry Climate Measure Research Both All Efficient Water Heating All ARBI (DEG) Challenge Home Production Builder Implementation Community Scale Verification 40% New All Integrated Design

  11. Sandia National Laboratories for the United States

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    XrayToolKit.com X-Ray Toolkit (XTK) is a software program developed by Sandia National Laboratories for the United States Government under funding from NNSA and TSWG. XTK is a radiograph acquisition and processing program designed specifically for Explosive Ordnance Disposal (EOD) technicians. XTK supports image acquisition from a variety of commercial scanners and provides image enhancement, measurement, and markup tools through a modern and user- friendly interface. Other features include

  12. Social Media | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Information for Media » Social Media Social Media Join us for a discussion of this year's SunShot Summit during the May 7 #SolarChat! Register today! RSS feed icon. For the latest news, subscribe to our RSS feeds. Facebook icon. Like us on Facebook. Twitter icon. Or follow us on Twitter; hashtag for the event is #SunShotSummit. Home About Agenda Registration Venue & Travel Attendee Resources Information for Media Media Kit News Social Media

  13. BC Hydro Brings Energy Savings to Low-Income Families in Canada |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy BC Hydro Brings Energy Savings to Low-Income Families in Canada BC Hydro Brings Energy Savings to Low-Income Families in Canada The number of British Columbia, Canada, households eligible for Better Buildings Residential Network member BC Hydro's Energy Conservation Assistance Program (ECAP) just doubled. British Columbia Energy Minister Bill Bennett recently announced an increase in the low-income qualification cutoff for BC Hydro's free home energy-saving kits and

  14. Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe

    DOEpatents

    Lucas, Joe N.; Straume, Tore; Bogen, Kenneth T.

    2000-01-01

    A method for detecting and isolating a target sequence in a sample of nucleic acids is provided using a bifunctional hybridization probe capable of hybridizing to the target sequence that includes a detectable marker and a first complexing agent capable of forming a binding pair with a second complexing agent. A kit is also provided for detecting a target sequence in a sample of nucleic acids using a bifunctional hybridization probe according to this method.

  15. PowerPoint Presentation

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    sba.gov www.sba.gov HUBZone Program Overview Department of Energy Office of Small and Disadvantaged Business Utilization Business Opportunity Session Mariana Pardo Director HUBZone Program July 29, 2013 www.sba.gov What We'll Cover * HUBZone - - - history and purpose * Requirements - Certification - Keeping the certification * Contract Types (benefits) * Question & Answer Session 3 www.sba.gov Quick History - Why is this important? * In 1996, U.S. Senator Christopher 'Kit' Bond, then

  16. Los Alamos scientists detect and track single molecules with nanoscale

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    carbon cylinders Nanotube "glowsticks" transform surface science tool kit Los Alamos scientists detect and track single molecules with nanoscale carbon cylinders Researchers have now shown that semiconducting carbon nanotubes have the potential to detect and track single molecules in water. January 10, 2012 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering

  17. MADNESS | Argonne Leadership Computing Facility

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Software & Libraries Boost CPMD Code_Saturne GAMESS GPAW GROMACS LAMMPS MADNESS QBox IBM References Cooley Policies Documentation Feedback Please provide feedback to help guide us as we continue to build documentation for our new computing resource. [Feedback Form] MADNESS Overview MADNESS is a numerical tool kit used to solve integral differential equations using multi-resolution analysis and a low-rank separation representation. MADNESS can solve multi-dimensional equations, currently up

  18. News

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    CAMD News LSU Researcher discovers a key step in photosynthesis at CAMD, earns cover on Journal of Biological Chemistry nanowerk article features CAMD's EFRC research Impact of Deepwater Horizon Oil Spill Evaluated at CAMD Dr. Challa Kumar builds "Millifluidic Catalyst Test Kit" MBP Cancer Center Receives Contract for Radiation Therapy (pdf) CAMD articles in the Baton Rouge Advocate EFRC and CAMD featured in Chemistry World (pdf) Mezzo Receives Indy Award (pdf) LSU Daily Reveille CAMD

  19. Newsroom

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    NIF & Photon Science News Press Releases Experimental Highlights Efficiency Improvements Science & Technology Meetings and Workshops Papers and Presentations NIF&PS People In the News Press Kit S&TR Articles home / news Newsroom × Subscribe to our News Alerts * Your Email Address: * Preferred Format: HTML Text Subscribe NIF & Photon Science News Links to the latest updates from NIF & Photon Science Press Releases LLNL news releases about NIF&PS activities

  20. Teachers Invited to April 1 Science Activities Night at Jefferson Lab; Sign

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    up by March 18 | Jefferson Lab 1 Science Activities Night at Jefferson Lab; Sign up by March 18 Teachers participated in hands-on science activities and picked up activities' starter kits and resource materials at Jefferson Lab's 2014 Teachers' Night. The event focused on physical science activities that teachers of fourth- through eighth-grade classes can use in the classroom. Dozens of teachers, including participants in the JLab Science Activities for Teachers program, shared their

  1. Teachers Invited to April 20 Science Activities Night at Jefferson Lab |

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Jefferson Lab 0 Science Activities Night at Jefferson Lab Teachers participated in hands-on science activities and picked up activities' starter kits and resource materials at Jefferson Lab's 2010 Teachers' Night. The event focused on physical science activities that teachers of fourth- through eighth-grade classes can use in the classroom. More than three dozen teachers, participants in the JLab Science Activities for Teachers program, shared their favorite or most-effective classroom

  2. Method and compositions for detecting of bloodstains using fluorescin-fluorescein reaction

    DOEpatents

    Di Benedetto, John; Kyle, Kevin; Boan, Terry; Marie, Charlene

    2004-02-17

    A method, compositions and kit are set forth for detecting blood stains. A reactant solution includes fluorescin solubilized (reduced) in acetic acid in ethanol. The solution may be buffered to a pH of approximately 9. After spraying the reactant solution on the suspected area an oxidizer is applied to promote the fluorescin to fluorescein reaction with the blood. The reacted fluorescein is then detected through luminescence for capture by photography.

  3. CALiPER Exploratory Study. Recessed Troffer Lighting

    SciTech Connect

    Miller, N. J.; Royer, M. P.; Poplawski, M. E.

    2013-03-01

    This CALiPER study examines the problems and benefits likely to be encountered with LED products intended to replace linear fluorescent lamps. LED dedicated troffers, replacement tubes, and non-tube retrofit kits were evaluated against fluorescent benchmark troffers in a simulated office space for photometric distribution, uniformity of light on the task surface, suitability of light output, flicker, dimming performance, color quality, power quality, safety and certification issues, ease of installation, energy efficiency, and life-cycle cost.

  4. MEMORANDUM To: DOE Office of General Counsel From: Tony Bacon

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    MEMORANDUM To: DOE Office of General Counsel From: Tony Bacon Date: August 6, 2015 Re: Ex Parte Communication Memo This memo provides a summary of the communications made to the DOE staff regarding proposed rules on ceiling fan test procedures as well as proposed energy efficiency rules for ceiling fan light kits. The communications occurred during a teleconference on July 30, 2015. The meeting attendees included: Lucy Debutts (DOE) Ashley Armstrong (DOE) David Turner (Hunter Fan Company) Tony

  5. Multiplex detection of agricultural pathogens

    DOEpatents

    McBride, Mary Teresa; Slezak, Thomas Richard; Messenger, Sharon Lee

    2010-09-14

    Described are kits and methods useful for detection of seven agricultural pathogens (BPSV; BHV; BVD; FMDV; BTV; SVD; and VESV) in a sample. Genomic sequence information from 7 agricultural pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.

  6. Scanning Cargo Containers with Tagged Neutrons

    SciTech Connect

    Viesti, G.; Botosso, C.; Fabris, D.; Lunardon, M.; Moretto, S.; Nebbia, G.; Pesente, S.; Zenoni, A.; Donzella, A.; Perot, B.; Carasco, C.; Bernard, S.; Mariani, A.; Szabo, J.-L.; Sannie, G.; Valkovic, V.; Sudac, D.; Nad, K.; Peerani, P.; Sequeira, V.

    2007-10-26

    A new Tagged Neutron Inspection System (TNIS) able to detect illicit materials such as explosives and narcotics in cargo containers has been developed within the EURopean Illicit TRAfficing Countermeasures Kit (EURITRACK) project. After the R and D phase, the inspection portal has been installed and commissioned at the Rijeka seaport in Croatia, where it has been operated in connection with the existing X-ray scanner for a first two-month demonstration campaign. Results obtained are presented and discussed in this paper.

  7. OpenStudio Core Development and Deployment Support - 2014 BTO Peer Review |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy Core Development and Deployment Support - 2014 BTO Peer Review OpenStudio Core Development and Deployment Support - 2014 BTO Peer Review Presenter: Larry Brackney, National Renewable Energy Laboratory The U.S. Department of Energy's (DOE's) energy analysis platform, OpenStudio, has made construction and analysis of building energy models easier and faster. The OpenStudio software development kit (SDK) also enables national laboratory and university researchers, along

  8. Agouti polynucleotide compositions and methods of use

    DOEpatents

    Woychik, Richard P. (Orinda, CA); Bultman, Scott J. (Lakewood, OH); Michaud, Edward J. (Kingston, TN)

    2003-02-04

    Disclosed are methods and compositions comprising novel agouti polypeptides and the polynucleotides which encode them. Also disclosed are DNA segments encoding these proteins derived from human and murine cell lines, and the use of these polynucleotides and polypeptides in a variety of diagnostic and therapeutic applications. Methods, compositions, kits, and devices are also provided for identifying compounds which are inhibitors of agouti activity, and for altering fatty acid synthetase activity and intracellular calcium levels in transformed cells.

  9. Agouti polypeptide compositions

    DOEpatents

    Woychik, Richard P. (Orinda, CA); Bultman, Scott J. (Lakewood, OH); Michaud, Edward J. (Kingston, TN)

    2001-10-30

    Disclosed are methods and compositions comprising novel agouti polypeptides and the polynucleotides which encode them. Also disclosed are DNA segments encoding these proteins derived from human and murine cell lines, and the use of these polynucleotides and polypeptides in a variety of diagnostic and therapeutic applications. Methods, compositions, kits, and devices are also provided for identifying compounds which are inhibitors of agouti activity, and for altering fatty acid synthetase activity and intracellular calcium levels in transformed cells.

  10. Metal aminoboranes

    DOEpatents

    Burrell, Anthony K.; Davis, Benjamin J.; Thorn, David L.; Gordon, John C.; Baker, R. Thomas; Semelsberger, Troy Allen; Tumas, William; Diyabalanage, Himashinie Vichalya Kaviraj; Shrestha, Roshan P.

    2010-05-11

    Metal aminoboranes of the formula M(NH.sub.2BH.sub.3).sub.n have been synthesized. Metal aminoboranes are hydrogen storage materials. Metal aminoboranes are also precursors for synthesizing other metal aminoboranes. Metal aminoboranes can be dehydrogenated to form hydrogen and a reaction product. The reaction product can react with hydrogen to form a hydrogen storage material. Metal aminoboranes can be included in a kit.

  11. Daniel Cohen Assistant General Counsel for Legislation and Regulatory Law

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Daniel Cohen Assistant General Counsel for Legislation and Regulatory Law Office of the General Counsel Department of Energy 1000 Independence Avenue, SW Washington, DC 20585-0121 Re: Ex Parte Memorandum Dear Mr. Cohen: On Tuesday, May 4, 2010, Kit Kennedy and Kate Houren of the Natural Resources Defense Council (NRDC) initiated a telephone call with Daniel Cohen and Bryan Miller of the Department of Energy's Office of the General Council. During the call, NRDC advocated for the adoption of the

  12. Transformative Wave Presentation - Volttron 2016

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Volttron for Small and Medium Buildings Justin Sipe SR VP of Technology Justin@twavetech.com Agenda *A private sector view on Volttron *Why do we continue to invest *What's changed in a year *What's stayed the same *What's needed for the future 2 About Transformative Wave * A subsidiary of the Performance Mechanical Group * 30 year + design build mechanical contractor * 80 + total employees * Founded in 2009 as the technology development * Primary product, THE CATALYST, is a retrofit kit for

  13. Methods and compositions for targeting macromolecules into the nucleus

    DOEpatents

    Chook, Yuh Min

    2013-06-25

    The present invention includes compositions, methods and kits for directing an agent across the nuclear membrane of a cell. The present invention includes a Karyopherin beta2 translocation motif in a polypeptide having a slightly positively charged region or a slightly hydrophobic region and one or more R/K/H-X.sub.(2-5)-P-Y motifs. The polypeptide targets the agent into the cell nucleus.

  14. New Technologies | Y-12 National Security Complex

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    New Technologies New Technologies The National Security Technology Center has achieved notable successes in the design and development of innovative devices with wide-ranging national security applications. For more information, send an email to NSTC. Access Rate Control System Developed to support safeguards and security, ARCS is a fully mechanical, ready-to-install kit that controls the speed of a person's entry through full-height turnstiles. The higher the speed, the more force is generated

  15. Promote the use of electric and hybrid vehicles through information dissemination & data collection through the NESEA American Tour de Sol. Final project report

    SciTech Connect

    2000-09-12

    A report on information dissemination on alternative fueled vehicles and on efficiency data collected at the NESEA American Tour de Sol is presented. Some of the latest advanced transportation technology vehicles were showcased. Numerous attachments are included, such as the post-event newsletter, press kit, publicity report, results table, technical workshop proceedings, NESEA tour rules and resources, and a paper titled ''Quantifying the fuel use and greenhouse gas reduction potential of electric and hybrid vehicles.''

  16. Multiplex detection of agricultural pathogens

    SciTech Connect

    Siezak, Thomas R.; Gardner, Shea; Torres, Clinton; Vitalis, Elizabeth; Lenhoff, Raymond J.

    2013-01-15

    Described are kits and methods useful for detection of agricultural pathogens in a sample. Genomic sequence information from agricultural pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay and/or an array assay to successfully identify the presence or absence of pathogens in a sample.

  17. UNCLASSIFIED Institute for Materials Science Distinguished Lecture Series

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Doctor Peter Wölfle Professor Emeritus Institute for Theoretical Condensed Matter Physics (KIT) The Exotic World of Quantum Matter: Novel States Induced by Fluctuations Tuesday, June 30, 2015 2 - 3pm MSL Auditorium (TA-03 - Bldg 1698 - Room A103) Abstract: The talk reviews established concepts of quantum matter and more recently discovered unexpected properties leading beyond. The low energy excitations of quantum matter generally have particle-like character. However, the character of these

  18. Experimental Highlights - 2014

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    NIF & Photon Science News Press Releases Experimental Highlights Efficiency Improvements Science & Technology Meetings and Workshops Papers and Presentations NIF&PS People In the News Press Kit S&TR Articles home / news / experimental highlights / 2014 Experimental Highlights - 2014 December NIF's TARDIS Aims to Conquer Time and Space November DIXI Reveals New Hot-Spot Details Controlling Hydrodynamic Mixing in NIF Implosions MIT's Mighty-Mite Detectors Prove Their Mettle October

  19. The Harnessed Atom | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Services » The Harnessed Atom The Harnessed Atom The Harnessed Atom The Harnessed Atom is a new middle school science, technology, engineering, and math (STEM) curriculum extension that focuses on nuclear science and energy. It offers teachers accurate, unbiased, and up-to-date information on the roles that energy and nuclear science play in our lives. The curriculum includes essential principles and fundamental concepts of energy science. This teacher's kit is an updated and expanded edition

  20. NEAMS Quarterly Report April-June 2013 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    3 NEAMS Quarterly Report April-June 2013 The Nuclear Energy Advanced Modeling and Simulation (NEAMS) quarterly report includes highlights, fuel and reactor product line accomplishments, recent and upcoming milestones, news on BISON fuel benchmarks, the latest MeshKit release features, and information on numerical simulations of pebble-bed reactor cores performed by the thermal hydraulics team. NEAMSQuarterly - April - June13-Report.pdf (1005.75 KB) More Documents & Publications NEAMS

  1. CX-100291 Categorical Exclusion Determination | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    1 Categorical Exclusion Determination CX-100291 Categorical Exclusion Determination Final Rulemaking Regarding Energy Conservation Standards for Packaged Terminal Air Conditioners RIN: 1904-AC82 CX(s) Applied: B5.1 EERE- Buildings Technology Program Date: 06/017/2015 Location(s): Nationwide Office(s): Golden Field Office In this proposed rule, DOE proposes new and amended energy conservation standards for ceiling fan light kits. Doe has determined that the amended energy conservation standards

  2. NREL: Workforce Development and Education Programs - Workforce Development

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    and Education Programs Staff Workforce Development and Education Programs Staff Contact the Workforce Development and Education Programs staff for questions on internships, graduate programs, competitions, teacher programs, and education initiatives. Photo of a woman sitting on the floor, assembling a wind turbine in front of a fan. Energy Institute participants use KidWind wind turbine kits to accomplish tasks such as pumping water, lifting weights, and charging a capacitor. The Energy

  3. JavaCleaner - Energy Innovation Portal

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Industrial Technologies Industrial Technologies Find More Like This Return to Search JavaCleaner Sandia National Laboratories Contact SNL About This Technology Publications: PDF Document Publication Market Sheet (341 KB) Technology Marketing Summary When installing new versions of Java, preexisting versions remain on your system, presenting significant security risks. Currently available Java cleanup tools are web-based, ignore installations of the Java Development Kit (JDK), detect only those

  4. Jefferson Lab Public Affairs

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Electronic Media print version Public Affairs Links Home Journalists' Newsroom Media Photographic Archives What is Jefferson Lab? Community Outreach Public Affairs Director's Office - Media The Public Affairs Office is prepared to aid media representatives in preparing for JLab stories and photo sessions. We can also help reporters find expert sources for science and technology-based stories. Media kits, provided upon request, offer a wealth of JLab information including fact sheets, photos,

  5. Hot Topics Workshops | Photosynthetic Antenna Research Center

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Hot Topics Workshops Hot Topics Workshops PARC partners with Institute for School Partnership to offer a series of "Hot Topics" workshops. The Hot Topics workshop series are focused on energy and sustainability for grade 6-12 teachers. All workshops are supported with written Instructional Materials and Energy Kits, a comprehensive bin of materials necessary to complete the investigations in your classroom. Pilot materials were supported by Toshiba. If you have any questions, please

  6. How to Work with the Media | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    How to Work with the Media How to Work with the Media How to Work with the Media, slides presented during a Better Buildings webinar on April 27, 2011. How to Work with the Media (2.96 MB) More Documents & Publications April 27, 2011 - How to Work with the Media Webinar (text version) SEP and EECBG program communications resource kit Marketing & Driving Demand Collaborative - Social Media Tools & Strategies

  7. Methods for detection, identification and specification of listerias

    DOEpatents

    Bochner, Barry

    1992-01-01

    The present invention relates generally to differential carbon source metabolism in the genus Listeria, metabolic, biochemical, immunological and genetic procedures to measure said differential carbon source metabolism and the use of these produces to detect, isolate and/or distinguish species of the genus Listeria as well as detect, isolate and/or distinguish strains of species of Listeria. The present invention also contemplates test kits and enrichment media to facilitate these procedures.

  8. Mobile on-site sample collection, preparation, and analysis in Iraq. Final report, January-April 1995

    SciTech Connect

    Swahn, I.D.; Brzezinski, J.H.

    1996-11-01

    The U.S. Army Edgewood Research, Development and Engineering Center has developed mobile on-site sample collection, preparation, and analysis equipment to collect environmental samples in highly contaminated areas. This equipment is being used by the United Nations Special Commission at the Baghdad Monitoring and Verification Center (BMVC), which provides long-term monitoring of dual-purpose chemical sites in Iraq, especially those with potential for chemical warfare (CW) production. A mobile laboratory was set-up in the BMVC to prepare and analyze samples collected throughout Iraq. Automatic air samplers were installed at various sites to collect vapor samples on absorption tubes that were analyzed using a gas chromatographic (GC) flame photometric detector (FPD). Mobile sample collection kits were used to collect solid, liquid, air, and wipe samples during challenge inspections. These samples were prepared using a sample preparation kit, which concentrates CW agent, breakdown products, and their precursors in complex matrices down to sub part per million levels for chemical analysis by a GC mass selective detector (MSD). This report describes the problems and solutions encountered with setting up a self-sufficient mobile analytical laboratory. Details of the various components associated with the laboratory and the collection kits are included.

  9. FIELD-DEPLOYABLE SAMPLING TOOLS FOR SPENT NUCLEAR FUEL INTERROGATION IN LIQUID STORAGE

    SciTech Connect

    Berry, T.; Milliken, C.; Martinez-Rodriguez, M.; Hathcock, D.; Heitkamp, M.

    2012-09-12

    Methodology and field deployable tools (test kits) to analyze the chemical and microbiological condition of aqueous spent fuel storage basins and determine the oxide thickness on the spent fuel basin materials were developed to assess the corrosion potential of a basin. this assessment can then be used to determine the amount of time fuel has spent in a storage basin to ascertain if the operation of the reactor and storage basin is consistent with safeguard declarations or expectations and assist in evaluating general storage basin operations. The test kit was developed based on the identification of key physical, chemical and microbiological parameters identified using a review of the scientific and basin operations literature. The parameters were used to design bench scale test cells for additional corrosion analyses, and then tools were purchased to analyze the key parameters. The tools were used to characterize an active spent fuel basin, the Savannah River Site (SRS) L-Area basin. The sampling kit consisted of a total organic carbon analyzer, an YSI multiprobe, and a thickness probe. The tools were field tested to determine their ease of use, reliability, and determine the quality of data that each tool could provide. Characterization confirmed that the L Area basin is a well operated facility with low corrosion potential.

  10. RGG: Reactor geometry (and mesh) generator

    SciTech Connect

    Jain, R.; Tautges, T.

    2012-07-01

    The reactor geometry (and mesh) generator RGG takes advantage of information about repeated structures in both assembly and core lattices to simplify the creation of geometry and mesh. It is released as open source software as a part of the MeshKit mesh generation library. The methodology operates in three stages. First, assembly geometry models of various types are generated by a tool called AssyGen. Next, the assembly model or models are meshed by using MeshKit tools or the CUBIT mesh generation tool-kit, optionally based on a journal file output by AssyGen. After one or more assembly model meshes have been constructed, a tool called CoreGen uses a copy/move/merge process to arrange the model meshes into a core model. In this paper, we present the current state of tools and new features in RGG. We also discuss the parallel-enabled CoreGen, which in several cases achieves super-linear speedups since the problems fit in available RAM at higher processor counts. Several RGG applications - 1/6 VHTR model, 1/4 PWR reactor core, and a full-core model for Monju - are reported. (authors)

  11. A new coal-permeability model: Internal swelling stress and fracture-matrix interaction

    SciTech Connect

    Liu, H.H.; Rutqvist, J.

    2009-10-01

    We have developed a new coal-permeability model for uniaxial strain and constant confining stress conditions. The model is unique in that it explicitly considers fracture-matrix interaction during coal deformation processes and is based on a newly proposed internal-swelling stress concept. This concept is used to account for the impact of matrix swelling (or shrinkage) on fracture-aperture changes resulting from partial separation of matrix blocks by fractures that do not completely cut through the whole matrix. The proposed permeability model is evaluated with data from three Valencia Canyon coalbed wells in the San Juan Basin, where increased permeability has been observed during CH{sub 4} gas production, as well as with published data from laboratory tests. Model results are generally in good agreement with observed permeability changes. The importance of fracture-matrix interaction in determining coal permeability, demonstrated in this work using relatively simple stress conditions, underscores the need for a dual-continuum (fracture and matrix) mechanical approach to rigorously capture coal-deformation processes under complex stress conditions, as well as the coupled flow and transport processes in coal seams.

  12. DNA-PK assay

    DOEpatents

    Anderson, Carl W.; Connelly, Margery A.

    2004-10-12

    The present invention provides a method for detecting DNA-activated protein kinase (DNA-PK) activity in a biological sample. The method includes contacting a biological sample with a detectably-labeled phosphate donor and a synthetic peptide substrate defined by the following features to provide specific recognition and phosphorylation by DNA-PK: (1) a phosphate-accepting amino acid pair which may include serine-glutamine (Ser-Gln) (SQ), threonine-glutamine (Thr-Gln) (TQ), glutamine-serine (Gln-Ser) (QS), or glutamine-threonine (Gln-Thr) (QT); (2) enhancer amino acids which may include glutamic acid or glutamine immediately adjacent at the amino- or carboxyl- side of the amino acid pair and forming an amino acid pair-enhancer unit; (3) a first spacer sequence at the amino terminus of the amino acid pair-enhancer unit; (4) a second spacer sequence at the carboxyl terminus of the amino acid pair-enhancer unit, which spacer sequences may include any combination of amino acids that does not provide a phosphorylation site consensus sequence motif; and, (5) a tag moiety, which may be an amino acid sequence or another chemical entity that permits separating the synthetic peptide from the phosphate donor. A compostion and a kit for the detection of DNA-PK activity are also provided. Methods for detecting DNA, protein phosphatases and substances that alter the activity of DNA-PK are also provided. The present invention also provides a method of monitoring protein kinase and DNA-PK activity in living cells. -A composition and a kit for monitoring protein kinase activity in vitro and a composition and a kit for monitoring DNA-PK activities in living cells are also provided. A method for identifying agents that alter protein kinase activity in vitro and a method for identifying agents that alter DNA-PK activity in living cells are also provided.

  13. Current parallel I/O limitations to scalable data analysis.

    SciTech Connect

    Mascarenhas, Ajith Arthur; Pebay, Philippe Pierre

    2011-07-01

    This report describes the limitations to parallel scalability which we have encountered when applying our otherwise optimally scalable parallel statistical analysis tool kit to large data sets distributed across the parallel file system of the current premier DOE computational facility. This report describes our study to evaluate the effect of parallel I/O on the overall scalability of a parallel data analysis pipeline using our scalable parallel statistics tool kit [PTBM11]. In this goal, we tested it using the Jaguar-pf DOE/ORNL peta-scale platform on a large combustion simulation data under a variety of process counts and domain decompositions scenarios. In this report we have recalled the foundations of the parallel statistical analysis tool kit which we have designed and implemented, with the specific double intent of reproducing typical data analysis workflows, and achieving optimal design for scalable parallel implementations. We have briefly reviewed those earlier results and publications which allow us to conclude that we have achieved both goals. However, in this report we have further established that, when used in conjuction with a state-of-the-art parallel I/O system, as can be found on the premier DOE peta-scale platform, the scaling properties of the overall analysis pipeline comprising parallel data access routines degrade rapidly. This finding is problematic and must be addressed if peta-scale data analysis is to be made scalable, or even possible. In order to attempt to address these parallel I/O limitations, we will investigate the use the Adaptable IO System (ADIOS) [LZL+10] to improve I/O performance, while maintaining flexibility for a variety of IO options, such MPI IO, POSIX IO. This system is developed at ORNL and other collaborating institutions, and is being tested extensively on Jaguar-pf. Simulation code being developed on these systems will also use ADIOS to output the data thereby making it easier for other systems, such as ours, to

  14. Endangered species and cultural resources program, Naval Petroleum Reserves in California: Annual report FY95

    SciTech Connect

    1996-04-01

    In FY95, EG and G Energy Measurements, Inc. (EG and G/EM) continued to support efforts to protect endangered species and cultural resources at the Naval Petroleum Reserves in California (NPRC). These efforts are conducted to ensure NPRC compliance with regulations regarding the protection of listed species and cultural resources on Federal properties. Population monitoring activities are conducted annually for San Joaquin kit foxes, giant kangaroo rats, blunt-nosed leopard lizards, and Hoover`s wooly-star. To mitigate impacts of oil field activities on listed species, 674 preactivity surveys covering approximately 211 hectares (521 acres) were conducted in FY95. EG and G/EM also assisted with mitigating effects from third-party projects, primarily by conducting biological and cultural resource consultations with regulatory agencies. EG and G/EM has conducted an applied habitat reclamation program at NPRC since 1985. In FY95, an evaluation of revegetation rates on reclaimed and non-reclaimed disturbed lands was completed, and the results will be used to direct future habitat reclamation efforts at NPRC. In FY95, reclamation success was monitored on 50 sites reclaimed in 1985. An investigation of factors influencing the distribution and abundance of kit foxes at NPRC was initiated in FY94. Factors being examined include habitat disturbance, topography, grazing, coyote abundance, lagomorph abundance, and shrub density. This investigation continued in FY95 and a manuscript on this topic will be completed in FY96. Also, Eg and G/EM completed collection of field data to evaluate the effects of a well blow-out on plant and animal populations. A final report will be prepared in FY96. Finally, EG and G/EM completed a life table analysis on San Joaquin kit foxes at NPRC.

  15. The potential benefits of nicaraven to protect against radiation-induced injury in hematopoietic stem/progenitor cells with relative low dose exposures

    SciTech Connect

    Ali, Haytham; Galal, Omima; Urata, Yoshishige; Goto, Shinji; Guo, Chang-Ying; Luo, Lan; Abdelrahim, Eman; Ono, Yusuke; Mostafa, Emtethal; Li, Tao-Sheng

    2014-09-26

    Highlights: Nicaraven mitigated the radiation-induced reduction of c-kit{sup +} stem cells. Nicaraven enhanced the function of hematopoietic stem/progenitor cells. Complex mechanisms involved in the protection of nicaraven to radiation injury. - Abstract: Nicaraven, a hydroxyl radical-specific scavenger has been demonstrated to attenuate radiation injury in hematopoietic stem cells with 5 Gy ?-ray exposures. We explored the effect and related mechanisms of nicaraven for protecting radiation injury induced by sequential exposures to a relatively lower dose ?-ray. C57BL/6 mice were given nicaraven or placebo within 30 min before exposure to 50 mGy ?-ray daily for 30 days in sequences (cumulative dose of 1.5 Gy). Mice were victimized 24 h after the last radiation exposure, and the number, function and oxidative stress of hematopoietic stem cells were quantitatively estimated. We also compared the gene expression in these purified stem cells from mice received nicaraven and placebo treatment. Nicaraven increased the number of c-kit{sup +} stem/progenitor cells in bone marrow and peripheral blood, with a recovery rate around 6090% of age-matched non-irradiated healthy mice. The potency of colony forming from hematopoietic stem/progenitor cells as indicator of function was completely protected with nicaraven treatment. Furthermore, nicaraven treatment changed the expression of many genes associated to DNA repair, inflammatory response, and immunomodulation in c-kit{sup +} stem/progenitor cells. Nicaraven effectively protected against damages of hematopoietic stem/progenitor cells induced by sequential exposures to a relatively low dose radiation, via complex mechanisms.

  16. H5Hut

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Hut H5Hut Description and Overview H5hut (HDF5 Utility Toolkit) is a veneer API for HDF5: H5hut files are also valid HDF5 files and are compatible with other HDF5-based interfaces and tools. For example, the h5dump tool that comes standard with HDF5 can export H5hut files to ASCII or XML for additional portability. H5hut also includes tools to convert H5hut data to the Visualization ToolKit (VTK) format and to generate scripts for the GNUplot data plotting tool. Main modules: H5Part:

  17. International Conference

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    & Engineering (M&C 2013) Sun Valley, Idaho, USA, May 5-9, 2013, on CD-ROM, American Nuclear Society, LaGrange Park, IL (2013) THE DATA TRANSFER KIT: A GEOMETRIC RENDEZVOUS-BASED TOOL FOR MULTIPHYSICS DATA TRANSFER S.R. Slattery and P.P.H. Wilson Department of Engineering Physics University of Wisconsin - Madison 1500 Engineering Dr., Madison, WI 53706 sslattery@wisc.edu; wilsonp@engr.wisc.edu R.P. Pawlowski Sandia National Laboratories ∗ P.O. Box 5800, Albuquerque, NM 87185

  18. Cask weeping mitigation

    DOEpatents

    Krumhansl, James L. (Albuquerque, NM); Brady, Patrick V. (Albuquerque, NM); Teter, David M. (Edgewood, NM); McConnell, Paul (Albuquerque, NM)

    2007-09-18

    A method (and concomitant kit) for treating a surface to reduce subsequent .sup.137Cs nuclide desorption comprising contacting the surface with a first cation-containing solution, the cation being one or more of Cs.sup.+, Rb.sup.+, Ag.sup.+, Tl.sup.+, K.sup.+, and NH.sub.4.sup.+, and contacting the surface with a second cation-containing solution, the cation being one or more of Cs.sup.+, Rb.sup.+, Ag.sup.+, Tl.sup.+, K.sup.+, and NH.sub.4.sup.+, thereby reducing amounts of radioactive cesium embedded in clays found on the surface.

  19. Volatile organic compound sensor system

    DOEpatents

    Schabron, John F.; Rovani, Jr., Joseph F.; Bomstad, Theresa M.; Sorini-Wong, Susan S.

    2009-02-10

    Generally, this invention relates to the development of field monitoring methodology for new substances and sensing chemical warfare agents (CWAs) and terrorist substances. It also relates to a portable test kit which may be utilized to measure concentrations of halogenated volatile organic compounds (VOCs) in the field. Specifically it relates to systems for reliably field sensing the potential presence of such items while also distinguishing them from other elements potentially present. It also relates to overall systems and processes for sensing, reacting, and responding to an indicated presence of such substance, including modifications of existing halogenated sensors and arrayed sensing systems and methods.

  20. SEMINAR ANNOUNCEMENTS

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Seminar Series Doctor Peter Wölfle Professor Emeritus Ins8tute for Theore8cal Condensed Ma=er Physics (KIT) Quantum Cri8cality in Heavy Fermion Compounds in the Absence of "Kondo breakdown" Thursday, July 7, 2016 2 - 3 pm Sig Hecker Conference Room (TA3 - 0032 room 134) Abstract: Unusual quantum cri-cal proper-es observed on several heavy fermion compounds have revived specula-on on a "Kondo breakdown" scenario. We show that in two prominent cases, YbRh2Si2 and CeCu6(Au), a

  1. SNLSimMagic v 2.0

    SciTech Connect

    2015-10-21

    This software is an iOS (Apple) Augmented Reality (AR) application that runs on the iPhone and iPad. It is designed to scan in a photograph or graphic and “play” an associated video. This release, SNLSimMagic, was built using Wikitude Augmented Reality (AR) software development kit (SDK) integrated into Apple iOS SDK application and the Cordova libraries. These codes enable the generation of runtime targets using cloud recognition and developer-defined target features which are then accessed by means of a custom application.

  2. SNLSimMagic v 2.0

    Energy Science and Technology Software Center

    2015-10-21

    This software is an iOS (Apple) Augmented Reality (AR) application that runs on the iPhone and iPad. It is designed to scan in a photograph or graphic and “play” an associated video. This release, SNLSimMagic, was built using Wikitude Augmented Reality (AR) software development kit (SDK) integrated into Apple iOS SDK application and the Cordova libraries. These codes enable the generation of runtime targets using cloud recognition and developer-defined target features which are then accessedmore » by means of a custom application.« less

  3. Network-based collaborative research environment LDRD final report

    SciTech Connect

    Davies, B.R.; McDonald, M.J.

    1997-09-01

    The Virtual Collaborative Environment (VCE) and Distributed Collaborative Workbench (DCW) are new technologies that make it possible for diverse users to synthesize and share mechatronic, sensor, and information resources. Using these technologies, university researchers, manufacturers, design firms, and others can directly access and reconfigure systems located throughout the world. The architecture for implementing VCE and DCW has been developed based on the proposed National Information Infrastructure or Information Highway and a tool kit of Sandia-developed software. Further enhancements to the VCE and DCW technologies will facilitate access to other mechatronic resources. This report describes characteristics of VCE and DCW and also includes background information about the evolution of these technologies.

  4. OSTI contributes to Second Harvest | OSTI, US Dept of Energy Office of

    Office of Scientific and Technical Information (OSTI)

    Scientific and Technical Information contributes to Second Harvest Back to the OSTI News Listing for 2006 OSTI recently provided 28 "Food for Kids" kits and 3 Backpacks to the Second Harvest Food Bank of Knoxville, as part of a food drive sponsored by the Oak Ridge Chapter of the Federally Employed Women (FEW). The "Food for Kids" program provides backpacks filled with nutritious, non-perishable food. The local "Food for Kids" drive provides food for over 1,600

  5. Enhanced citric acid production in aspergillus with inactivated asparagine-linked glycosylation protein 3 (ALG3), and/or increased laeA expression

    SciTech Connect

    Dai, Ziyu; Baker, Scott E.

    2015-12-08

    Provided herein are fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (Lae), or both. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also provided, as are compositions and kits including the disclosed fungi.

  6. Method for isolating nucleic acids

    SciTech Connect

    Hurt, Jr., Richard Ashley; Elias, Dwayne A.

    2015-09-29

    The current disclosure provides methods and kits for isolating nucleic acid from an environmental sample. The current methods and compositions further provide methods for isolating nucleic acids by reducing adsorption of nucleic acids by charged ions and particles within an environmental sample. The methods of the current disclosure provide methods for isolating nucleic acids by releasing adsorbed nucleic acids from charged particles during the nucleic acid isolation process. The current disclosure facilitates the isolation of nucleic acids of sufficient quality and quantity to enable one of ordinary skill in the art to utilize or analyze the isolated nucleic acids for a wide variety of applications including, sequencing or species population analysis.

  7. Biomarkers for liver fibrosis

    SciTech Connect

    Jacobs, Jon M.; Burnum-Johnson, Kristin E.; Baker, Erin M.; Smith, Richard D.; Gritsenko, Marina A.; Orton, Daniel

    2015-09-15

    Methods and systems for diagnosing or prognosing liver fibrosis in a subject are provided. In some examples, such methods and systems can include detecting liver fibrosis-related molecules in a sample obtained from the subject, comparing expression of the molecules in the sample to controls representing expression values expected in a subject who does not have liver fibrosis or who has non-progressing fibrosis, and diagnosing or prognosing liver fibrosis in the subject when differential expression of the molecules between the sample and the controls is detected. Kits for the diagnosis or prognosis of liver fibrosis in a subject are also provided which include reagents for detecting liver fibrosis related molecules.

  8. OpenStudio: An Open Source Integrated Analysis Platform; Preprint

    SciTech Connect

    Guglielmetti, R.; Macumber, D.; Long, N.

    2011-12-01

    High-performance buildings require an integrated design approach for all systems to work together optimally; systems integration needs to be incorporated in the earliest stages of design for efforts to be cost and energy-use effective. Building designers need a full-featured software framework to support rigorous, multidisciplinary building simulation. An open source framework - the OpenStudio Software Development Kit (SDK) - is being developed to address this need. In this paper, we discuss the needs that drive OpenStudio's system architecture and goals, provide a development status report (the SDK is currently in alpha release), and present a brief case study that illustrates its utility and flexibility.

  9. DNA sequencing with pyrophosphatase

    DOEpatents

    Tabor, S.; Richardson, C.C.

    1996-03-12

    A kit or solution is disclosed for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule and having a second single-stranded region homologous to the first single-stranded region. The first agent is able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture. The second agent is able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.

  10. Florida County Helping Homeowners Save Energy and Money | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Energy Florida County Helping Homeowners Save Energy and Money Florida County Helping Homeowners Save Energy and Money March 9, 2011 - 1:23pm Addthis Jennifer Holman Project Officer, Golden Field Office What does this project do? Sarasota County is offering one of the three following energy efficient improvements to its citizens. (1) Free Do-It-Yourself home energy kits. (2) Rebates of up to $3,750 on approved energy saving home improvements audits performed by approved contractors. (3) Zero

  11. Chlorine resistant desalination membranes based on directly sulfonated poly(arylene ether sulfone) copolymers

    DOEpatents

    McGrath, James E.; Park, Ho Bum; Freeman, Benny D.

    2011-10-04

    The present invention provides a membrane, kit, and method of making a hydrophilic-hydrophobic random copolymer membrane. The hydrophilic-hydrophobic random copolymer membrane includes a hydrophilic-hydrophobic random copolymer. The hydrophilic-hydrophobic random copolymer includes one or more hydrophilic monomers having a sulfonated polyarylsulfone monomer and a second monomer and one or more hydrophobic monomers having a non-sulfonated third monomer and a fourth monomer. The sulfonated polyarylsulfone monomer introduces a sulfonate into the hydrophilic-hydrophobic random copolymer prior to polymerization.

  12. Current induced perpendicular-magnetic-anisotropy racetrack memory with magnetic field assistance

    SciTech Connect

    Zhang, Y.; Klein, J.-O.; Chappert, C.; Ravelosona, D.; Zhao, W. S.

    2014-01-20

    High current density is indispensable to shift domain walls (DWs) in magnetic nanowires, which limits the using of racetrack memory (RM) for low power and high density purposes. In this paper, we present perpendicular-magnetic-anisotropy (PMA) Co/Ni RM with global magnetic field assistance, which lowers the current density for DW motion. By using a compact model of PMA RM and 40 nm design kit, we perform mixed simulation to validate the functionality of this structure and analyze its density potential. Stochastic DW motion behavior has been taken into account and statistical Monte-Carlo simulations are carried out to evaluate its reliability performance.

  13. Project Earth Lover

    SciTech Connect

    Slobotski, Stephanie,

    2011-09-01

    Under this project, the Ponca Tribe of Nebraska (PTN) will conduct An Energy Options Analysis (EOA) to empower Tribal Leadership with critical information to allow them to effectively screen energy options that will further develop the Tribe's long-term strategic plan and energy vision. The PTN will also provide community workshops to enhance Tribal Members' capabilities, skills and awareness of energy efficiency and conservation technology and practices. A 90- minute workshop will be conducted at each of the 5 sites and one-hundred tribal members will receive an erergy efficiency kit.

  14. The Web as an API.

    SciTech Connect

    Roman, J. H.

    2001-01-01

    As programmers we have worked with many Application Development Interface API development kits. They are well suited for interaction with a particular system. A vast source of information can be made accessible by using the http protocol through the web as an API. This setup has many advantages including the vast knowledge available on setting web servers and services. Also, these tools are available on most hardware and operating system combinations. In this paper I will cover the various types of systems that can be developed this way, their advantages and some drawbacks of this approach. Index Terms--Application Programmer Interface, Distributed applications, Hyper Text Transfer Protocol, Web.

  15. Beam simulation tools for GEANT4 (and neutrino source applications)

    SciTech Connect

    V.Daniel Elvira, Paul Lebrun and Panagiotis Spentzouris

    2002-12-03

    Geant4 is a tool kit developed by a collaboration of physicists and computer professionals in the High Energy Physics field for simulation of the passage of particles through matter. The motivation for the development of the Beam Tools is to extend the Geant4 applications to accelerator physics. Although there are many computer programs for beam physics simulations, Geant4 is ideal to model a beam going through material or a system with a beam line integrated to a complex detector. There are many examples in the current international High Energy Physics programs, such as studies related to a future Neutrino Factory, a Linear Collider, and a very Large Hadron Collider.

  16. Real Time Technology Application Demonstration Project Final Report

    SciTech Connect

    Crumbling, Deana

    2008-04-02

    Each of the 20 1-kg sample bags of Paducah soil will be sampled using a multi-increment (MI) strategy. These MI samples will be analyzed for total PCBs by the Abraxis PCB-HC kit. Processed soil samples will also be pooled to create sufficient volume for analysis for PAHs and PCB Aroclors by the ERT-Edison laboratory. Methanol extracts from Abraxis extractions will be analyzed by the ERT-Edison lab, the Abraxis lab, and the XDS laboratory for TEQ analytes.

  17. DNA sequencing with pyrophosphatase

    DOEpatents

    Tabor, Stanley; Richardson, Charles C.

    1996-03-12

    A kit or solution for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule having a second single-stranded region homologous to the first single-stranded region, comprising a first agent able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture, and a second agent able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.

  18. Testing your home for lead in paint, dust, and soil

    SciTech Connect

    1998-10-01

    This publication is for anyone who is considering having a home or residence tested for lead in paint, dust, or soil by a professional. It explains the technical aspects of lead testing without overwhelming the reader. The first section tells why you would test for lead, the approaches for testing for lead, and what information you will get from each approach. The second section answers specific questions about how paint, soil, and dust sampling are conducted by the professional in your home. Finally, the last section answers other questions about testing, including questions about home test kits and testing of water and ceramics.

  19. Volatile organic compound sensor system

    DOEpatents

    Schabron, John F.; Rovani, Jr., Joseph F.; Bomstad, Theresa M.; Sorini-Wong, Susan S.; Wong, Gregory K.

    2011-03-01

    Generally, this invention relates to the development of field monitoring methodology for new substances and sensing chemical warfare agents (CWAs) and terrorist substances. It also relates to a portable test kit which may be utilized to measure concentrations of halogenated volatile organic compounds (VOCs) in the field. Specifically it relates to systems for reliably field sensing the potential presence of such items while also distinguishing them from other elements potentially present. It also relates to overall systems and processes for sensing, reacting, and responding to an indicated presence of such substance, including modifications of existing halogenated sensors and arrayed sensing systems and methods.

  20. Second Line of Defense Master Spares Catalog

    SciTech Connect

    Henderson, Dale L.; Muller, George; Mercier, Theresa M.; Brigantic, Robert T.; Perkins, Casey J.; Cooley, Scott K.

    2012-11-20

    This catalog is intended to be a comprehensive listing of repair parts, components, kits, and consumable items used on the equipment deployed at SLD sites worldwide. The catalog covers detection, CAS, network, ancillary equipment, and tools. The catalog is backed by a Master Parts Database which is used to generate the standard report views of the catalog. The master parts database is a relational database containing a record for every part in the master parts catalog along with supporting tables for normalizing fields in the records. The database also includes supporting queries, database maintenance forms, and reports.

  1. Dechlorination of TCE with palladized iron

    DOEpatents

    Fernando, Q.; Muftikian, R.; Korte, N.

    1997-03-18

    The present invention relates to various methods, such as an above-ground method and an in-ground method, of using a palladized iron bimetallic system for the dechlorination of chlorinated organic compounds from effluents containing the same. The use of palladized iron bimetallic system results in the dechlorination of the chlorinated organic compound into environmentally safe reaction products. The present invention also provides kits, devices, and other instruments that use the above-mentioned palladized iron bimetallic system for the dechlorination of chlorinated organic compounds. 10 figs.

  2. Functionalized expanded porphyrins

    DOEpatents

    Sessler, Jonathan L; Pantos, Patricia J

    2013-11-12

    Disclosed are functionalized expanded porphyrins that can be used as spectrometric sensors for high-valent actinide cations. The disclosed functionalized expanded porphyrins have the advantage over unfunctionalized systems in that they can be immobilized via covalent attachment to a solid support comprising an inorganic or organic polymer or other common substrates. Substrates comprising the disclosed functionalized expanded porphyrins are also disclosed. Further, disclosed are methods of making the disclosed compounds (immobilized and free), methods of using them as sensors to detect high valent actinides, devices that comprise the disclosed compounds, and kits.

  3. HEMLOCK

    Energy Science and Technology Software Center

    2009-03-25

    HEMLOCK (Heterogeneous Ensemble Machine Learning Open Classification Kit) is a software tool for constructing, evaluating, and applying heterogeneous ensemble data models for use in solving supervised machine learning problems. Specifically, the main class of problems targeted by HEMLOCK is the problem of multiple-class classification (also called labeling or categorization) of data with continuous or discrete features. HEMLOCK consists of various data readers, machine learning algorithms, model combination and comparison routines, evaluation methods for model performancemore » testing, and interfaces to external, state-of-the-art machine learning software libraries.« less

  4. Decontamination formulations for disinfection and sterilization

    DOEpatents

    Tucker, Mark D.; Engler, Daniel E.

    2007-09-18

    Aqueous decontamination formulations that neutralize biological pathogens for disinfection and sterilization applications. Examples of suitable applications include disinfection of food processing equipment, disinfection of areas containing livestock, mold remediation, sterilization of medical instruments and direct disinfection of food surfaces, such as beef carcasses. The formulations include at least one reactive compound, bleaching activator, inorganic base, and water. The formulations can be packaged as a two-part kit system, and can have a pH value in the range of 7-8.

  5. High throughput protein production screening

    DOEpatents

    Beernink, Peter T.; Coleman, Matthew A.; Segelke, Brent W.

    2009-09-08

    Methods, compositions, and kits for the cell-free production and analysis of proteins are provided. The invention allows for the production of proteins from prokaryotic sequences or eukaryotic sequences, including human cDNAs using PCR and IVT methods and detecting the proteins through fluorescence or immunoblot techniques. This invention can be used to identify optimized PCR and WT conditions, codon usages and mutations. The methods are readily automated and can be used for high throughput analysis of protein expression levels, interactions, and functional states.

  6. High-stringency screening of target-binding partners using a microfluidic device

    DOEpatents

    Soh, Hyongsok; Lou, Xinhui; Lagally, Eric

    2015-12-01

    The invention provides a method of screening a library of candidate agents by contacting the library with a target in a reaction mixture under a condition of high stringency, wherein the target includes a tag that responds to a controllable force applied to the tag, and passing the members of the library through a microfluidic device in a manner that exposes the library members to the controllable force, thereby displacing members of the library that are bound to the target relative to their unbound counterparts. Kits and systems for use with the methods of the invention are also provided.

  7. Dechlorination of TCE with palladized iron

    DOEpatents

    Fernando, Q.; Muftikian, R.; Korte, N.

    1998-06-02

    The present invention relates to various methods, such as an above-ground method and an in-ground method, of using a palladized iron bimetallic system for the dechlorination of chlorinated organic compounds from effluents containing the same. The use of palladized iron bimetallic system results in the dechlorination of the chlorinated organic compound into environmentally safe reaction products. The present invention also provides kits, devices, and other instruments that use the above-mentioned palladized iron bimetallic system for the dechlorination of chlorinated organic compounds. 10 figs.

  8. Consortium for Advanced Simulation of Light Water Reactors (CASL)

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Media Kit CASL Acknowledgement This research was supported by the Consortium for Advanced Simulation of Light Water Reactors (http://www.casl.gov), an Energy Innovation Hub (http://www.energy.gov/hubs) for Modeling and Simulation of Nuclear Reactors under U.S. Department of Energy Contract No. DE-AC05-00OR22725. CASL Logo Files CASL Extended - CASL_word.jpg and CASL_word.png CASL without words - CASL.jpg and CASL.png CASL with words - CASL_word.jpg and CASL_word.png CASL Partners - partners.jpg

  9. KrakenOverview

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    of Kraken Daniel Lucio User Support Joint Cray XT5 Workshop Feb 1-3 2010, Berkeley, CA Outline 1. Basics 2. How to 3. NICS Survival Kit 4. Important Policies 5. Docs & Reference & Help 6. Q&A 1. Basics * NICS is a collaboration between UT and ORNL * Awarded the NSF Track 2B ($65M) * Phased deployment of Cray XT systems * Staffed with 25 FTEs * Total JICS funding ~$92M National Institute for Computational Sciences Kraken's Timeline XT3 XT4 Initial XT5 Final XT5 April 08 July 08 Feb

  10. Multiplex detection of respiratory pathogens

    DOEpatents

    McBride, Mary; Slezak, Thomas; Birch, James M.

    2012-07-31

    Described are kits and methods useful for detection of respiratory pathogens (influenza A (including subtyping capability for H1, H3, H5 and H7 subtypes) influenza B, parainfluenza (type 2), respiratory syncytial virus, and adenovirus) in a sample. Genomic sequence information from the respiratory pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.

  11. Use of Galerina marginata genes and proteins for peptide production

    DOEpatents

    Hallen-Adams, Heather E.; Scott-Craig, John S.; Walton, Jonathan D.; Luo, Hong

    2016-03-01

    The present invention relates to compositions and methods comprising genes and peptides associated with cyclic peptides and cyclic peptide production in mushrooms. In particular, the present invention relates to using genes and proteins from Galerina species encoding peptides specifically relating to amatoxins in addition to proteins involved with processing cyclic peptide toxins. In a preferred embodiment, the present invention also relates to methods for making small peptides and small cyclic peptides including peptides similar to amanitin. Further, the present inventions relate to providing kits for making small peptides.

  12. Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination

    DOEpatents

    Xie, Jianming; Wang, Lei; Wu, Ning; Schultz, Peter G.

    2008-07-15

    Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino acids. Methods of determining the structure of a protein, e.g., a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.

  13. Final Technical Report: Hydrogen Energy in Engineering Education (H2E3)

    SciTech Connect

    Lehman, Peter A.; Cashman, Eileen; Lipman, Timothy; Engel, Richard A.

    2011-09-15

    Schatz Energy Research Center's Hydrogen Energy in Engineering Education curriculum development project delivered hydrogen energy and fuel cell learning experiences to over 1,000 undergraduate engineering students at five California universities, provided follow-on internships for students at a fuel cell company; and developed commercializable hydrogen teaching tools including a fuel cell test station and a fuel cell/electrolyzer experiment kit. Monitoring and evaluation tracked student learning and faculty and student opinions of the curriculum, showing that use of the curriculum did advance student comprehension of hydrogen fundamentals. The project web site (hydrogencurriculum.org) provides more information.

  14. Influence of physiography and vegetation on small mammals at the Naval Petroleum Reserves, California

    SciTech Connect

    Cypher, B.L.

    1995-02-13

    Influence of physiography and vegetation on small mammal abundance and species Composition was investigated at Naval Petroleum Reserve No. 1 in California to assess prey abundance for Federally endangered San Joaquin kit foxes (Vulpes macrotis mutica) and to assess the distribution of two Federal candidate species, San Joaquin antelope squirrels (Ammospermophilus nelsoni) and short-nosed kangaroo rats (Dinodomys nitratoides brevinasus). The specific objectives of this investigation were to determine whether small mammal abundance and community composition varied with north-south orientation, terrain, ground cover, and Cypher shrub density, and whether these factors influenced the distribution and abundance of San Joaquin antelope squirrels and short-nosed kangaroo rats.

  15. Dechlorination of TCE with palladized iron

    DOEpatents

    Fernando, Quintus; Muftikian, Rosy; Korte, Nic

    1997-01-01

    The present invention relates to various methods, such as an above-ground method and an in-ground method, of using a palladized iron bimetallic system for the dechlorination of chlorinated organic compounds from effluents containing the same. The use of palladized iron bimetallic system results in the dechlorination of the chlorinated organic compound into environmentally safe reaction products. The present invention also provides kits, devices, and other instruments that use the above-mentioned palladized iron bimetallic system for the dechlorination of chlorinated organic compounds.

  16. Dechlorination of TCE with palladized iron

    DOEpatents

    Fernando, Quintus; Muftikian, Rosy; Korte, Nic

    1998-01-01

    The present invention relates to various methods, such as an above-ground method and an in-ground method, of using a palladized iron bimetallic system for the dechlorination of chlorinated organic compounds from effluents containing the same. The use of palladized iron bimetallic system results in the dechlorination of the chlorinated organic compound into environmentally safe reaction products. The present invention also provides kits, devices, and other instruments that use the above-mentioned palladized iron bimetallic system for the dechlorination of chlorinated organic compounds.

  17. 11121-5302-01 - Project Management Plan - 07-25-14

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Final Technical Report 2016-07-11 SMT © 2016 Page 1 of 127 Project Title: Deepwater Subsea Pressure Compensated Chemical Reservoir (RPSEA project 11121-5302-01) 2016-07-10 SMT - The SMarT Solution(tm) Enabling long-distance subsea tie-backs - 3000 BBL chemical storage & injection (eliminate chemical umbilical) - Subsea pig launcher (eliminate 2 nd flowline) Platform for enabling Brownfield EOR - Seafloor placement of 'kit' enabling shuttle to - from surface for IRM Safe Marine Transfer, LLC

  18. Biogass Generator

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Another internet tool by: Build Your Own Page 1 of 5 Teach...build...learn...renewable energy! Biogas Generator A Renewable Energy Project Kit The Pembina Institute What Is Biogas? Biogas is actually a mixture of gases, usually carbon dioxide and methane. It is produced by a few kinds of microorganisms, usually when air or oxygen is absent. (The absence of oxygen is called "anaerobic conditions.") Animals that eat a lot of plant material, particularly grazing animals such as cattle,

  19. Probes for anionic cell surface detection

    DOEpatents

    Smith, Bradley D.

    2013-03-05

    Embodiments of the present invention are generally directed to compositions comprising a class of molecular probes for detecting the presence of anionic cell surfaces. Embodiments include compositions that are enriched for these compositions and preparations, particularly preparations suitable for use as laboratory/clinical reagents and diagnostic indicators, either alone or as part of a kit. An embodiment of the invention provides for a highly selective agent useful in the discernment and identification of dead or dying cells, such as apoptotic cells, in a relatively calcium-free environment. An embodiment of the invention provides a selective agent for the identification of bacteria in a mixed population of bacterial cells and nonbacterial cells.

  20. Methods of biological dosimetry employing chromosome-specific staining

    DOEpatents

    Gray, Joe W.; Pinkel, Daniel

    2000-01-01

    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods are provided to disable the hybridization capacity of shared, high copy repetitive sequences and/or remove such sequences to provide for useful contrast. Still further methods are provided to produce chromosome-specific staining reagents which are made specific to the targeted chromosomal material, which can be one or more whole chromosomes, one or more regions on one or more chromosomes, subsets of chromosomes and/or the entire genome. Probes and test kits are provided for use in tumor cytogenetics, in the detection of disease related loci, in analysis of structural abnormalities, such as translocations, and for biological dosimetry. Further, methods and prenatal test kits are provided to stain targeted chromosomal material of fetal cells, including fetal cells obtained from maternal blood. Still further, the invention provides for automated means to detect and analyse chromosomal abnormalities.

  1. Compositions for chromosome-specific staining

    DOEpatents

    Gray, J.W.; Pinkel, D.

    1998-05-26

    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. The methods produce staining patterns that can be tailored for specific cytogenetic analyses. The probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods are provided to disable the hybridization capacity of shared, high copy repetitive sequences and/or remove such sequences to provide for useful contrast. Still further methods are provided to produce chromosome-specific staining reagents which are made specific to the targeted chromosomal material, which can be one or more whole chromosomes, one or more regions on one or more chromosomes, subsets of chromosomes and/or the entire genome. Probes and test kits are provided for use in tumor cytogenetics, in the detection of disease related loci, in analysis of structural abnormalities, such as translocations, and for biological dosimetry. Methods and prenatal test kits are provided to stain targeted chromosomal material of fetal cells, including fetal cells obtained from maternal blood. The invention provides for automated means to detect and analyze chromosomal abnormalities. 17 figs.

  2. Compositions for chromosome-specific staining

    DOEpatents

    Gray, Joe W.; Pinkel, Daniel

    1998-01-01

    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods are provided to disable the hybridization capacity of shared, high copy repetitive sequences and/or remove such sequences to provide for useful contrast. Still further methods are provided to produce chromosome-specific staining reagents which are made specific to the targeted chromosomal material, which can be one or more whole chromosomes, one or more regions on one or more chromosomes, subsets of chromosomes and/or the entire genome. Probes and test kits are provided for use in tumor cytogenetics, in the detection of disease related loci, in analysis of structural abnormalities, such as translocations, and for biological dosimetry. Further, methods and prenatal test kits are provided to stain targeted chromosomal material of fetal cells, including fetal cells obtained from maternal blood. Still further, the invention provides for automated means to detect and analyse chromosomal abnormalities.

  3. Automated analysis of failure event data

    SciTech Connect

    HENNESSY,COREY; FREERKS,FRED; CAMPBELL,JAMES E.; THOMPSON,BRUCE M.

    2000-03-27

    This paper focuses on fully automated analysis of failure event data in the concept and early development stage of a semiconductor-manufacturing tool. In addition to presenting a wide range of statistical and machine-specific performance information, algorithms have been developed to examine reliability growth and to identify major contributors to unreliability. These capabilities are being implemented in a new software package called Reliadigm. When coupled with additional input regarding repair times and parts availability, the analysis software also provides spare parts inventory optimization based on genetic optimization methods. The type of question to be answered is: If this tool were placed with a customer for beta testing, what would be the optimal spares kit to meet equipment reliability goals for the lowest cost? The new algorithms are implemented in Windows{reg_sign} software and are easy to apply. This paper presents a preliminary analysis of failure event data from three IDEA machines currently in development. The paper also includes an optimal spare parts kit analysis.

  4. Submission of Final Scientific/Technical Report [Solar Avoided Cost Solution: SunShot 6 Final Report

    SciTech Connect

    Danziger, Eric

    2014-01-29

    The core objectives of this project were two separate but integrated products, collectively providing game-changing Avoided Cost capabilities. • The first was a kit of avoided cost tools and data that any solar provider can use a-lacarte or as a whole. It’s open and easily accessible nature allows the rapid and accurate calculation of avoided cost in whatever context and software that make sense (“Typical and Avoided Cost Tools”). This kit includes a dataset of typical energy rates, costs and usage that can be used for solar prospecting, lead generation and any situation where data about an opportunity is missing or imperfect. • The second is a web application and related APIs specifically built for solar providers to radically streamline their lead-to-sale process (“Solar Provider Module”). The typical and Avoided Cost tools are built directly into this, and allow for solar providers to track their opportunities, collaborate with their installers and financiers, and close more sales faster.

  5. Methods And Compositions For Chromosome-Specific Staining

    DOEpatents

    Gray, Joe W.; Pinkel, Daniel

    2003-08-19

    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods are provided to disable the hybridization capacity of shared, high copy repetitive sequences and/or remove such sequences to provide for useful contrast. Still further methods are provided to produce chromosome-specific staining reagents which are made specific to the targeted chromosomal material, which can be one or more whole chromosomes, one or more regions on one or more chromosomes, subsets of chromosomes and/or the entire genome. Probes and test kits are provided for use in tumor cytogenetics, in the detection of disease related loci, in analysis of structural abnormalities, such as translocations, and for biological dosimetry. Further, methods and prenatal test kits are provided to stain targeted chromosomal material of fetal cells, including fetal cells obtained from maternal blood. Still further, the invention provides for automated means to detect and analyse chromosomal abnormalities.

  6. Reference Model 2: %22Rev 0%22 Rotor Design.

    SciTech Connect

    Barone, Matthew F.; Berg, Jonathan Charles; Griffith, Daniel

    2011-12-01

    The preliminary design for a three-bladed cross-flow rotor for a reference marine hydrokinetic turbine is presented. A rotor performance design code is described, along with modifications to the code to allow prediction of blade support strut drag as well as interference between two counter-rotating rotors. The rotor is designed to operate in a reference site corresponding to a riverine environment. Basic rotor performance and rigid-body loads calculations are performed to size the rotor elements and select the operating speed range. The preliminary design is verified with a simple finite element model that provides estimates of bending stresses during operation. A concept for joining the blades and support struts is developed and analyzed with a separate finite element analysis. Rotor mass, production costs, and annual energy capture are estimated in order to allow calculations of system cost-of-energy. Evaluation Only. Created with Aspose.Pdf.Kit. Copyright 2002-2011 Aspose Pty Ltd Evaluation Only. Created with Aspose.Pdf.Kit. Copyright 2002-2011 Aspose Pty Ltd

  7. Habitat reclamation plan to mitigate for the loss of habitat due to oil and gas production activities under maximum efficient rate, Naval Petroleum Reserve No. 1, Kern County, California

    SciTech Connect

    Anderson, D.C.

    1994-11-01

    Activities associated with oil and gas development under the Maximum Efficiency Rate (MER) from 1975 to 2025 will disturb approximately 3,354 acres. Based on 1976 aerial photographs and using a dot grid methodology, the amount of land disturbed prior to MER is estimated to be 3,603 acres. Disturbances on Naval Petroleum Reserve No. 1 (NPR-1) were mapped using 1988 aerial photography and a geographical information system. A total of 6,079 acres were classified as disturbed as of June, 1988. The overall objective of this document is to provide specific information relating to the on-site habitat restoration program at NPRC. The specific objectives, which relate to the terms and conditions that must be met by DOE as a means of protecting the San Joaquin kit fox from incidental take are to: (1) determine the amount and location of disturbed lands on NPR-1 and the number of acres disturbed as a result of MER activities, (2) develop a long term (10 year) program to restore an equivalent on-site acres to that lost from prior project-related actions, and (3) examine alternative means to offset kit fox habitat loss.

  8. 2007 Wholesale Power Rate Case Initial Proposal : Risk Analysis Study Documentation.

    SciTech Connect

    United States. Bonneville Power Administration.

    2005-11-01

    The RiskMod Model is comprised of a set of risk simulation models, collectively referred to as RiskSim; a set of computer programs that manages data referred to as Data Management Procedures; and RevSim, a model that calculates net revenues. RiskMod interacts with the AURORA Model, the RAM2007, and the ToolKit Model during the process of performing the Risk Analysis Study. AURORA is the computer model being used to perform the Market Price Forecast Study (see Market Price Forecast Study, WP-07-E-BPA-03); the RAM2007 is the computer model being used to calculate rates (see Wholesale Power Rate Development Study, WP-07-E-BPA-05); and the ToolKit is the computer model being used to develop the risk mitigation package that achieves BPA's 92.6 percent TPP standard (see Section 3 in the Risk Analysis Study, WP-07-E-BPA-04). Variations in monthly loads, resources, natural gas prices, forward market electricity prices, transmission expenses, and aluminum smelter benefit payments are simulated in RiskSim. Monthly spot market electricity prices for the simulated loads, resources, and natural gas prices are estimated by the AURORA Model. Data Management Procedures facilitate the format and movement of data that flow to and/or from RiskSim, AURORA, and RevSim. RevSim estimates net revenues using risk data from RiskSim, spot market electricity prices from AURORA, loads and resources data from the Load Resource Study, WP-07-E-BPA-01, various revenues from the Revenue Forecast component of the Wholesale Power Rate Development Study, WP-07-E-BPA-05, and rates and expenses from the RAM2007. Annual average surplus energy revenues, purchased power expenses, and section 4(h)(10)(C) credits calculated by RevSim are used in the Revenue Forecast and the RAM2007. Heavy Load Hour (HLH) and Light Load Hour (LLH) surplus and deficit energy values from RevSim are used in the Transmission Expense Risk Model. Net revenues estimated for each simulation by RevSim are input into the ToolKit Model

  9. 2007 Wholesale Power Rate Case Final Proposal : Risk Analysis Study Documentation.

    SciTech Connect

    United States. Bonneville Power Administration.

    2006-07-01

    The RiskMod Model is comprised of a set of risk simulation models, collectively referred to as RiskSim; a set of computer programs that manages data referred to as Data Management Procedures; and RevSim, a model that calculates net revenues. RiskMod interacts with the AURORA Model, the RAM2007, and the ToolKit Model during the process of performing the Risk Analysis Study. AURORA is the computer model being used to perform the Market Price Forecast Study (see Market Price Forecast Study, WP-07-FS-BPA-03); the RAM2007 is the computer model being used to calculate rates (see Wholesale Power Rate Development Study, WP-07-FS-BPA-05); and the ToolKit is the computer model being used to develop the risk mitigation package that achieves BPA's 92.6 percent TPP standard (see Section 3 in the Risk Analysis Study, WP-07-FS-BPA-04). Variations in monthly loads, resources, natural gas prices, forward market electricity prices, transmission expenses, and aluminum smelter benefit payments are simulated in RiskSim. Monthly spot market electricity prices for the simulated loads, resources, and natural gas prices are estimated by the AURORA Model. Data Management Procedures facilitate the format and movement of data that flow to and/or from RiskSim, AURORA, and RevSim. RevSim estimates net revenues using risk data from RiskSim, spot market electricity prices from AURORA, loads and resources data from the Load Resource Study, WP-07-FS-BPA-01, various revenues from the Revenue Forecast component of the Wholesale Power Rate Development Study, WP-07-FSBPA-05, and rates and expenses from the RAM2007. Annual average surplus energy revenues, purchased power expenses, and section 4(h)(10)(C) credits calculated by RevSim are used in the Revenue Forecast and the RAM2007. Heavy Load Hour (HLH) and Light Load Hour (LLH) surplus and deficit energy values from RevSim are used in the Transmission Expense Risk Model. Net revenues estimated for each simulation by RevSim are input into the ToolKit

  10. Towards intelligent diagnostic system employing integration of mathematical and engineering model

    SciTech Connect

    Isa, Nor Ashidi Mat

    2015-05-15

    as mathematical model of clustering technique have been widely used in developing the medical diagnostic systems. The selected features will be classified using mathematical models that embedded engineering theory such as artificial intelligence, support vector machine, neural network and fuzzy-neuro system. These classifiers will provide the diagnostic results without human intervention. Among many publishable researches, several prototypes have been developed namely NeuralPap, Neural Mammo, and Cervix Kit. The former system (NeuralPap) is an automatic intelligent diagnostic system for classifying and distinguishing between the normal and cervical cancerous cells. Meanwhile, the Cervix Kit is a portable Field-programmable gate array (FPGA)-based cervical diagnostic kit that could automatically diagnose the cancerous cell based on the images obtained during sampling test. Besides the cervical diagnostic system, the Neural Mammo system is developed to specifically aid the diagnosis of breast cancer using a fine needle aspiration image.

  11. DEVELOPMENT OF METHODOLOGY AND FIELD DEPLOYABLE SAMPLING TOOLS FOR SPENT NUCLEAR FUEL INTERROGATION IN LIQUID STORAGE

    SciTech Connect

    Berry, T.; Milliken, C.; Martinez-Rodriguez, M.; Hathcock, D.; Heitkamp, M.

    2012-06-04

    This project developed methodology and field deployable tools (test kits) to analyze the chemical and microbiological condition of the fuel storage medium and determine the oxide thickness on the spent fuel basin materials. The overall objective of this project was to determine the amount of time fuel has spent in a storage basin to determine if the operation of the reactor and storage basin is consistent with safeguard declarations or expectations. This project developed and validated forensic tools that can be used to predict the age and condition of spent nuclear fuels stored in liquid basins based on key physical, chemical and microbiological basin characteristics. Key parameters were identified based on a literature review, the parameters were used to design test cells for corrosion analyses, tools were purchased to analyze the key parameters, and these were used to characterize an active spent fuel basin, the Savannah River Site (SRS) L-Area basin. The key parameters identified in the literature review included chloride concentration, conductivity, and total organic carbon level. Focus was also placed on aluminum based cladding because of their application to weapons production. The literature review was helpful in identifying important parameters, but relationships between these parameters and corrosion rates were not available. Bench scale test systems were designed, operated, harvested, and analyzed to determine corrosion relationships between water parameters and water conditions, chemistry and microbiological conditions. The data from the bench scale system indicated that corrosion rates were dependent on total organic carbon levels and chloride concentrations. The highest corrosion rates were observed in test cells amended with sediment, a large microbial inoculum and an organic carbon source. A complete characterization test kit was field tested to characterize the SRS L-Area spent fuel basin. The sampling kit consisted of a TOC analyzer, a YSI

  12. Triple helix purification and sequencing

    DOEpatents

    Wang, R.; Smith, L.M.; Tong, X.E.

    1995-03-28

    Disclosed herein are methods, kits, and equipment for purifying single stranded circular DNA and then using the DNA for DNA sequencing purposes. Templates are provided with an insert having a hybridization region. An elongated oligonucleotide has two regions that are complementary to the insert and the oligo is bound to a magnetic anchor. The oligo hybridizes to the insert on two sides to form a stable triple helix complex. The anchor can then be used to drag the template out of solution using a magnet. The system can purify sequencing templates, and if desired the triple helix complex can be opened up to a double helix so that the oligonucleotide will act as a primer for further DNA synthesis. 4 figures.

  13. Aptamer- and nucleic acid enzyme-based systems for simultaneous detection of multiple analytes

    DOEpatents

    Lu, Yi; Liu, Juewen

    2011-11-15

    The present invention provides aptamer- and nucleic acid enzyme-based systems for simultaneously determining the presence and optionally the concentration of multiple analytes in a sample. Methods of utilizing the system and kits that include the sensor components are also provided. The system includes a first reactive polynucleotide that reacts to a first analyte; a second reactive polynucleotide that reacts to a second analyte; a third polynucleotide; a fourth polynucleotide; a first particle, coupled to the third polynucleotide; a second particle, coupled to the fourth polynucleotide; and at least one quencher, for quenching emissions of the first and second quantum dots, coupled to the first and second reactive polynucleotides. The first particle includes a quantum dot having a first emission wavelength. The second particle includes a second quantum dot having a second emission wavelength different from the first emission wavelength. The third polynucleotide and the fourth polynucleotide are different.

  14. DAKOTA, a multilevel parallel object-oriented framework for design optimization, parameter estimation, uncertainty quantification, and sensitivity analysis:version 4.0 reference manual

    SciTech Connect

    Griffin, Joshua D. (Sandai National Labs, Livermore, CA); Eldred, Michael Scott; Martinez-Canales, Monica L.; Watson, Jean-Paul; Kolda, Tamara Gibson; Adams, Brian M.; Swiler, Laura Painton; Williams, Pamela J.; Hough, Patricia Diane; Gay, David M.; Dunlavy, Daniel M.; Eddy, John P.; Hart, William Eugene; Guinta, Anthony A.; Brown, Shannon L.

    2006-10-01

    The DAKOTA (Design Analysis Kit for Optimization and Terascale Applications) toolkit provides a flexible and extensible interface between simulation codes and iterative analysis methods. DAKOTA contains algorithms for optimization with gradient and nongradient-based methods; uncertainty quantification with sampling, reliability, and stochastic finite element methods; parameter estimation with nonlinear least squares methods; and sensitivity/variance analysis with design of experiments and parameter study methods. These capabilities may be used on their own or as components within advanced strategies such as surrogate-based optimization, mixed integer nonlinear programming, or optimization under uncertainty. By employing object-oriented design to implement abstractions of the key components required for iterative systems analyses, the DAKOTA toolkit provides a flexible and extensible problem-solving environment for design and performance analysis of computational models on high performance computers. This report serves as a reference manual for the commands specification for the DAKOTA software, providing input overviews, option descriptions, and example specifications.

  15. Automated diagnostic kiosk for diagnosing diseases

    DOEpatents

    Regan, John Frederick; Birch, James Michael

    2014-02-11

    An automated and autonomous diagnostic apparatus that is capable of dispensing collection vials and collections kits to users interesting in collecting a biological sample and submitting their collected sample contained within a collection vial into the apparatus for automated diagnostic services. The user communicates with the apparatus through a touch-screen monitor. A user is able to enter personnel information into the apparatus including medical history, insurance information, co-payment, and answer a series of questions regarding their illness, which is used to determine the assay most likely to yield a positive result. Remotely-located physicians can communicate with users of the apparatus using video tele-medicine and request specific assays to be performed. The apparatus archives submitted samples for additional testing. Users may receive their assay results electronically. Users may allow the uploading of their diagnoses into a central databank for disease surveillance purposes.

  16. Revisions to US EPA Superfund Risk and Dose Assessment Models and Guidance - 13403

    SciTech Connect

    Walker, Stuart A.

    2013-07-01

    The U.S. Environmental Protection Agency (EPA) Superfund program's six Preliminary Remediation Goal (PRG) and Dose Compliance Concentration (DCC) internet based calculators for risk and dose assessment at Superfund sites are being revised to reflect better science, revisions to existing exposure scenarios and new scenarios, and changes to match up more closely with the EPA chemical regional screening level calculator. A revised version of the 1999 guidance document that provides an overview for the Superfund risk assessment process at radioactively contaminated sites, 'Radiation Risk Assessment At CERCLA Sites: Q and A', is being completed that will reflect Superfund recommended guidance and other technical documents issued over the past 13 years. EPA is also issuing a series of fact sheets in the document 'Superfund Radiation Risk Assessment: A Community Tool-kit'. This presentation would go over those changes that are expected to be finished by this spring. (authors)

  17. Carboxy, carboalkoxy and carbamile substituted isonitrile radionuclide complexes

    DOEpatents

    Jones, Alun G.; Davison, Alan; Kronauge, James; Abrams, Michael J.

    1988-04-05

    A coordination complex comprising a radionuclide selected from the class consisting of radioactive isotopes of Tc, Ru, Co, Pt and Re and an isonitrile ligand of the formula: (CNX)R, where X is a lower alkyl group having 1 to 4 carbon atoms, wherein R is selected from the group consisting of COOR.sup.1 and CONR.sup.2 R.sup.3 where R.sup.1 can be H, a pharmaceutically acceptable cation, or a substituted or unsubstituted alkyl group having 1 to 4 carbon atoms, R.sup.2, and R.sup.3 can be H, or a substituted or unsubstituted alkyl group having 1 to 4 carbon atoms, and R.sup.2 and R.sup.3 can be the same or different is disclosed. Kits that can be used to form these complexes are also disclosed.

  18. Carboxy, carboalkoxy and carbamile substituted isonitrile radionuclide complexes

    DOEpatents

    Jones, Alun G.; Davison, Alan; Kronauge, James; Abrams, Michael J.

    1989-01-01

    A coordination complex comprising a radionuclide selected from the class consisting of radioactive isotopes of Tc, Ru, Co, Pt and Re and an isonitrile ligand of the formula: (CNX)R, where X is a lower alkyl group having 1 to 4 carbon atoms, wherein R is selected from the group consisting of COOR.sup.1 and CONR.sup.2 R.sup.3 where R.sup.1 can be H, a pharmaceutically acceptable cation, or a substituted or unsubstituted alkyl group having 1 to 4 carbon atoms. R.sup.2, and R.sup.3 can be H, or a substituted or unsubstituted alkyl group having 1 to 4 carbon atoms, and R.sup.2 and R.sup.3 can be the same of different is disclosed. Kits that can be used to form these complexes are also disclosed.

  19. Method of detecting genetic translocations identified with chromosomal abnormalities

    DOEpatents

    Gray, Joe W.; Pinkel, Daniel; Tkachuk, Douglas

    2001-01-01

    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML) and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.

  20. Chromosome-specific staining to detect genetic rearrangements associated with chromosome 3 and/or chromosome 17

    DOEpatents

    Gray, Joe W.; Pinkel, Daniel; Kallioniemi, Olli-Pekka; Kallioniemi, Anne; Sakamoto, Masaru

    2002-01-01

    Methods and compositions for staining based upon nucleic acid sequence that employ nudeic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML), retinoblastoma, ovarian and uterine cancers, and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.

  1. High-Performance Energy Applications and Systems

    SciTech Connect

    Miller, Barton

    2014-05-19

    The Paradyn project has a history of developing algorithms, techniques, and software that push the cutting edge of tool technology for high-end computing systems. Under this funding, we are working on a three-year agenda to make substantial new advances in support of new and emerging Petascale systems. The overall goal for this work is to address the steady increase in complexity of these petascale systems. Our work covers two key areas: (1) The analysis, instrumentation and control of binary programs. Work in this area falls under the general framework of the Dyninst API tool kits. (2) Infrastructure for building tools and applications at extreme scale. Work in this area falls under the general framework of the MRNet scalability framework. Note that work done under this funding is closely related to work done under a contemporaneous grant, “Foundational Tools for Petascale Computing”, SC0003922/FG02-10ER25940, UW PRJ27NU.

  2. Triple helix purification and sequencing

    DOEpatents

    Wang, Renfeng; Smith, Lloyd M.; Tong, Xinchun E.

    1995-01-01

    Disclosed herein are methods, kits, and equipment for purifying single stranded circular DNA and then using the DNA for DNA sequencing purposes. Templates are provided with an insert having a hybridization region. An elongated oligonucleotide has two regions that are complementary to the insert and the oligo is bound to a magnetic anchor. The oligo hybridizes to the insert on two sides to form a stable triple helix complex. The anchor can then be used to drag the template out of solution using a magnet. The system can purify sequencing templates, and if desired the triple helix complex can be opened up to a double helix so that the oligonucleotide will act as a primer for further DNA synthesis.

  3. A Divergence Statistics Extension to VTK for Performance Analysis.

    SciTech Connect

    Pebay, Philippe Pierre; Bennett, Janine Camille

    2015-02-01

    This report follows the series of previous documents ([PT08, BPRT09b, PT09, BPT09, PT10, PB13], where we presented the parallel descriptive, correlative, multi-correlative, principal component analysis, contingency, k -means, order and auto-correlative statistics engines which we developed within the Visualization Tool Kit ( VTK ) as a scalable, parallel and versatile statistics package. We now report on a new engine which we developed for the calculation of divergence statistics, a concept which we hereafter explain and whose main goal is to quantify the discrepancy, in a stasticial manner akin to measuring a distance, between an observed empirical distribution and a theoretical, "ideal" one. The ease of use of the new diverence statistics engine is illustrated by the means of C++ code snippets. Although this new engine does not yet have a parallel implementation, it has already been applied to HPC performance analysis, of which we provide an example.

  4. LNG: new driving force

    SciTech Connect

    Adkins, R.E.

    1981-11-01

    Spurred by recent legislation promoting the use of methane as a motor fuel, Beech Aircraft is gearing up for market production of a complete vehicular conversion kit and ground support equipment for a liquefied-methane fuel system that is suitable for the use of conventional LNG or methane collected from coalbeds, sewage plants, or landfills and liquefied on site. As demonstrated in field tests of prototype fuel systems, liquefied methane stores conveniently and is safe in motor vehicles. Compared with compressed methane, the liquefied form provides more horsepower and longer mileage between fuelings. Fully fueled, the Beech system weighs less than a gasoline or diesel tank of the same size. The system features electronic-capacitance gaging for direct dashboard quantity reading, a standby time of 14 days (from filling time until the time it reaches the maximum allowable vapor pressure of 60 psi), and the choice of vapor or liquid withdrawal.

  5. Apparatus for insulating windows and the like

    DOEpatents

    Mitchell, R.A.

    1984-06-19

    Apparatus for insulating window openings through walls and the like includes a thermal shutter, a rail for mounting the shutter adjacent to the window opening and a coupling for connecting the shutter to the rail. The thermal shutter includes an insulated panel adhered to frame members which surround the periphery of the panel. The frame members include a hard portion for providing the frame and a soft portion for providing a seal with that portion of the wall adjacent to the periphery of the opening. The coupling means is preferably integral with the attachment rail. According to a preferred embodiment, the coupling means includes a continuous hinge of reduced thickness. The thermal shutter can be permanently attached, hinged, bi-folded, or sliding with respect to the window and wall. A distribution method is to market the apparatus in kit'' form. 11 figs.

  6. MEK1-independent activation of MAPK and MEK1-dependent activation of p70 S6 kinase by stem cell factor (SCF) in ovarian cancer cells

    SciTech Connect

    Liu, Lian; Institute of Immunology, School of Medicine, Shandong University, Jinan 250012 ; Zhang, Xin; Du, Chao; Zhang, Xiaoning; Hou, Nan; Zhao, Di; Sun, Jianzhi; Li, Li; Wang, Xiuwen; Ma, Chunhong

    2009-05-01

    We discovered a stem cell factor (SCF)-triggered, MEK1-independent, and PI3K-dependent MAPK activation pathway in the Kit-expressing ovarian cancer cell line HEY. When we knocked down MEK1 with RNA interference (RNAi) to study the function of MEK1 on the proliferation and survival of ovarian cancer cells, we found that impaired cell growth still occurred after MEK1 expression had been suppressed, although MAPK activation remained intact. This suggests that there is MEK1-independent activation of MAPK in the SCF-induced ovarian cancer cell growth process, and that MEK1 still plays a crucial role in maintaining the malignant properties of ovarian cancer cells even when it fails to activate MAPK as expected.

  7. Of carrots and sticks or air power as a nonproliferation tool

    SciTech Connect

    Wolf, F.R.

    1994-07-01

    The proliferation of nuclear weapons has become one of the principal threats to international peace and security. Postwar revelations from Iraq demonstrate how close a determined nation can come to covertly developing nuclear weapons without detection. In the past two years the issue of nonproliferation has increased in importance and the regime is becoming more intrusive. On the other hand, a number of nations hostile to the international order are attempting to develop or otherwise obtain nuclear weapons These states include North Korea, Iran, and Iraq. This paper argues that the use or threat of force must be incorporated into the nonproliferation regime. When properly integrated into nonproliferation strategy, force offers positive effects in terms of deterrence, compellence, and defense. Thus, the paper calls for the institutionalization of force options into the nonproliferation tool kit, ideally as part of chapter 7 enforcement actions under the authority of the UN Security Council.

  8. Immunoassay panel profile for detecting total PCB content

    SciTech Connect

    Friedman, S.; Allen, R.; Gui, J.; Barren, E.; Berdahl, D.

    1995-12-31

    Immunoassay test kits are being widely used to provide rapid, inexpensive screening of soil samples for the presence of PCBs at or above a given threshold value. Currently available immunoassay methods are sensitive to aroclor preparations that contain the more highly chlorinated PCB congeners. The interpretation of these tests is accomplished by comparison to an appropriate aroclor standard. If PCB contamination at a site has undergone significant changes through weathering or biological degradation, or if contamination has occurred from lower chlorinated species, the relative sensitivity of available test methods is reduced. This paper describes the results of a program to develop and demonstrate an Immunoassay Panel Method that normalizes the recovery of PCBs detected and thereby provides an accurate representation of the total PCB content of a sample. The development and validation of this method, and the associative correlation testing data using laboratory and environmental samples, will be discussed.

  9. Solid phase sequencing of biopolymers

    DOEpatents

    Cantor, Charles R.; Hubert, Koster

    2014-06-24

    This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Probes may be affixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.

  10. Solid phase sequencing of biopolymers

    DOEpatents

    Cantor, Charles; Koster, Hubert

    2010-09-28

    This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include DNA or RNA in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.

  11. Foundational Tools for Petascale Computing

    SciTech Connect

    Miller, Barton

    2014-05-19

    The Paradyn project has a history of developing algorithms, techniques, and software that push the cutting edge of tool technology for high-end computing systems. Under this funding, we are working on a three-year agenda to make substantial new advances in support of new and emerging Petascale systems. The overall goal for this work is to address the steady increase in complexity of these petascale systems. Our work covers two key areas: (1) The analysis, instrumentation and control of binary programs. Work in this area falls under the general framework of the Dyninst API tool kits. (2) Infrastructure for building tools and applications at extreme scale. Work in this area falls under the general framework of the MRNet scalability framework. Note that work done under this funding is closely related to work done under a contemporaneous grant, “High-Performance Energy Applications and Systems”, SC0004061/FG02-10ER25972, UW PRJ36WV.

  12. Biomarkers for lymphoma

    DOEpatents

    Zangar, Richard C.; Varnum, Susan M.

    2014-09-02

    A biomarker, method, test kit, and diagnostic system for detecting the presence of lymphoma in a person are disclosed. The lymphoma may be Hodgkin's lymphoma or non-Hodgkin's lymphoma. The person may be a high-risk subject. In one embodiment, a plasma sample from a person is obtained. The level of at least one protein listed in Table S3 in the plasma sample is measured. The level of at least one protein in the plasma sample is compared with the level in a normal or healthy subject. The lymphoma is diagnosed based upon the level of the at least one protein in the plasma sample in comparison to the normal or healthy level.

  13. Activity-based assay for ricin-like toxins

    DOEpatents

    Keener, William K.; Ward, Thomas E.

    2007-02-06

    A method of detecting N-glycosylase activity in a sample involves incubating an oligodeoxyribonucleotide substrate containing a deoxyadenosine or deoxyuridine residue with the sample to be tested such that the N-glycosylase, if present, hydrolyzes the deoxyadenosine or deoxyuridine residue to result in an N-glycosylase product having an abasic site. A primer is annealed to the N-glycosylase product, and the primer is extended with a DNA polymerase, such as Taq DNA polymerase, that pauses at abasic sites. The resulting extension products are melted from the N-glycosylase product, allowed to form hairpins due to self-complementarity, and further extended in the presence of labeled precursors to result in labeled products. Extension products synthesized from undigested substrate as template do not result in labeled products. Thus, detection of labeled products results in detection of N-glycosylase activity. Oligodeoxyribonucleotide substrates, primer, and positive controls and a kit for N-glycosylase assay are also disclosed.

  14. RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes

    SciTech Connect

    Brettin, Thomas; Davis, James J.; Disz, Terry; Edwards, Robert A.; Gerdes, Svetlana; Olsen, Gary J.; Olson, Robert; Overbeek, Ross; Parrello, Bruce; Pusch, Gordon D.; Shukla, Maulik; Thomason, III, James A.; Stevens, Rick; Vonstein, Veronika; Wattam, Alice R.; Xia, Fangfang

    2015-02-10

    The RAST (Rapid Annotation using Subsystem Technology) annotation engine was built in 2008 to annotate bacterial and archaeal genomes. It works by offering a standard software pipeline for identifying genomic features (i.e., protein-encoding genes and RNA) and annotating their functions. Recently, in order to make RAST a more useful research tool and to keep pace with advancements in bioinformatics, it has become desirable to build a version of RAST that is both customizable and extensible. In this paper, we describe the RAST tool kit (RASTtk), a modular version of RAST that enables researchers to build custom annotation pipelines. RASTtk offers a choice of software for identifying and annotating genomic features as well as the ability to add custom features to an annotation job. RASTtk also accommodates the batch submission of genomes and the ability to customize annotation protocols for batch submissions. This is the first major software restructuring of RAST since its inception.

  15. Phase 1 Development Report for the SESSA Toolkit.

    SciTech Connect

    Knowlton, Robert G.; Melton, Brad J; Anderson, Robert J.

    2014-09-01

    The Site Exploitation System for Situational Awareness ( SESSA ) tool kit , developed by Sandia National Laboratories (SNL) , is a comprehensive de cision support system for crime scene data acquisition and Sensitive Site Exploitation (SSE). SESSA is an outgrowth of another SNL developed decision support system , the Building R estoration Operations Optimization Model (BROOM), a hardware/software solution for data acquisition, data management, and data analysis. SESSA was designed to meet forensic crime scene needs as defined by the DoD's Military Criminal Investigation Organiza tion (MCIO) . SESSA is a very comprehensive toolki t with a considerable amount of database information managed through a Microsoft SQL (Structured Query Language) database engine, a Geographical Information System (GIS) engine that provides comprehensive m apping capabilities, as well as a an intuitive Graphical User Interface (GUI) . An electronic sketch pad module is included. The system also has the ability to efficiently generate necessary forms for forensic crime scene investigations (e.g., evidence submittal, laboratory requests, and scene notes). SESSA allows the user to capture photos on site, and can read and generate ba rcode labels that limit transcription errors. SESSA runs on PC computers running Windows 7, but is optimized for touch - screen tablet computers running Windows for ease of use at crime scenes and on SSE deployments. A prototype system for 3 - dimensional (3 D) mapping and measur e ments was also developed to complement the SESSA software. The mapping system employs a visual/ depth sensor that captures data to create 3D visualizations of an interior space and to make distance measurements with centimeter - level a ccuracy. Output of this 3D Model Builder module provides a virtual 3D %22walk - through%22 of a crime scene. The 3D mapping system is much less expensive and easier to use than competitive systems. This document covers the basic installation and

  16. ETHERNET BASED EMBEDDED IOC FOR FEL CONTROL SYSTEM

    SciTech Connect

    Jianxun Yan; Daniel Sexton; Albert Grippo; Steven Moore; Kevin Jordan

    2008-01-23

    An Ethernet based embedded Input Output Controller (IOC) has been developed to upgrade the control system for the Free Electron Laser Project at Jefferson Lab. The embedded IOC, called the Single Board IOC (SBIOC), was integrated with a ColdFire embedded microprocessor and a Field Programmable Gate Array (FPGA) on a circuit board, which can be easily configured to control different kinds of I/O devices. The SBIOC provided features of a complete System-on-Module (SOM) as a stand alone system with abundant high speed I/O ports to couple with suitable devices. The software kits, Experimental Physics and Industrial Control System (EPICS) and Real Time Executive for Multiprocessor System (RTEMS), were chosen to work with our existing control system. The embedded IOC system has the features of a low cost IOC, free open source RTOS, plug-and-play-like ease of installation and flexibility.

  17. Rapid Application Development with OpenStudio: Preprint

    SciTech Connect

    Weaver, E.; Long, N.; Fleming, K.; Schott, M.; Benne, K.; Hale, E.

    2012-05-01

    This paper presents several case studies of rapidly implemented, audience-specific applications for whole building energy modeling and standards analysis. By tailoring each application to the audience and the task at hand, the required learning curve for new users was greatly reduced. Each case study used OpenStudio, the U.S. Department of Energy's middleware software development kit (SDK). OpenStudio provides an easy interface to the EnergyPlus whole building simulation engine, while extending its capability and providing higher-level functionality such as software interoperability, standards, analysis, and optimization. Each case study is unique in the technology employed to interface with OpenStudio as well as the methods used for user interaction and data presentation. Four case studies are presented.

  18. OPENSTUDIO�

    Energy Science and Technology Software Center

    2008-03-01

    OpenStudio� is a cross-platform (Windows, Mac, and Linux) collection of software tools to support whole building energy modeling using EnergyPlus and advanced daylight analysis using Radiance. OpenStudio is an open source (LGPL) project to facilitate community development, extension, and private sector adoption. OpenStudio includes graphical interfaces along with a Software Development Kit (SDK). In addition to the graphical interface, OpenStudio allows building researchers and software developers to quickly get started through its multiple entry levels,more » including access through C++, Ruby, and C#. Users can leverage the Ruby interface to create OpenStudio Measures that can be easily shared and applied to OpenStudio Models.« less

  19. Chromosome-specific staining to detect genetic rearrangements associated with chromosome 3 and/or chromosome 17

    DOEpatents

    Gray, Joe W.; Pinkel, Daniel; Kallioniemi, Olli-Pekka; Kallioniemi, Anne; Sakamoto, Masaru

    2009-10-06

    Methods and compositions for staining based upon nucleic acid sequence that employ .[.nudeic.]. .Iadd.nucleic .Iaddend.acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML), retinoblastoma, ovarian and uterine cancers, and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.

  20. Method of detecting genetic deletions identified with chromosomal abnormalities

    DOEpatents

    Gray, Joe W; Pinkel, Daniel; Tkachuk, Douglas

    2013-11-26

    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyzes. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acids probes are typically of a complexity greater tha 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particlularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML) and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar ut genetically different diseases, and for many prognostic and diagnostic applications.

  1. Chromosome-specific staining to detect genetic rearrangements

    DOEpatents

    Gray, Joe W.; Pinkel, Daniel; Tkachuk, Douglas; Westbrook, Carol

    2013-04-09

    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyzes. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML) and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.

  2. Intelligent Computing System for Reservoir Analysis and Risk Assessment of Red River Formation, Class Revisit

    SciTech Connect

    Sippel, Mark A.

    2002-09-24

    Integrated software was written that comprised the tool kit for the Intelligent Computing System (ICS). The software tools in ICS are for evaluating reservoir and hydrocarbon potential from various seismic, geologic and engineering data sets. The ICS tools provided a means for logical and consistent reservoir characterization. The tools can be broadly characterized as (1) clustering tools, (2) neural solvers, (3) multiple-linear regression, (4) entrapment-potential calculator and (5) combining tools. A flexible approach can be used with the ICS tools. They can be used separately or in a series to make predictions about a desired reservoir objective. The tools in ICS are primarily designed to correlate relationships between seismic information and data obtained from wells; however, it is possible to work with well data alone.

  3. Leveraging OpenStudio's Application Programming Interfaces: Preprint

    SciTech Connect

    Long, N.; Ball, B.; Goldwasser, D.; Parker, A.; Elling, J.; Davis, O.; Kruchten, D.

    2013-11-01

    OpenStudio development efforts have been focused on providing Application Programming Interfaces (APIs) where users are able to extend OpenStudio without the need to compile the open source libraries. This paper will discuss the basic purposes and functionalities of the core libraries that have been wrapped with APIs including the Building Model, Results Processing, Advanced Analysis, UncertaintyQuantification, and Data Interoperability through Translators. Several building energy modeling applications have been produced using OpenStudio's API and Software Development Kits (SDK) including the United States Department of Energy's Asset ScoreCalculator, a mobile-based audit tool, an energy design assistance reporting protocol, and a portfolio scale incentive optimization analysismethodology. Each of these software applications will be discussed briefly and will describe how the APIs were leveraged for various uses including high-level modeling, data transformations from detailed building audits, error checking/quality assurance of models, and use of high-performance computing for mass simulations.

  4. Nalu

    Energy Science and Technology Software Center

    2014-05-13

    Nalu is a Sierra ToolKit (STK) based application module, and it has provided a set of "lessons learned" for the STK transition effort through its early adoption of STK. It makes use of the open-sourced Trilinos/ Tpetra library. Through the investment of LORD and ASCR projects, the Nalu code module has been extended beyond prototype status. Physics capability includes low Mach, variable density turbulent flow. The ongoing objective for Nalu is to facilitate partnerships withmore » external organizations in order to extend code capability and knowledge; however, it is not intended to support routine CFD analysis. The targeted usage of this module is for non-NW applications that support work-for-others in the multiphysics energy sector.« less

  5. Nalu

    SciTech Connect

    2014-05-13

    Nalu is a Sierra ToolKit (STK) based application module, and it has provided a set of "lessons learned" for the STK transition effort through its early adoption of STK. It makes use of the open-sourced Trilinos/ Tpetra library. Through the investment of LORD and ASCR projects, the Nalu code module has been extended beyond prototype status. Physics capability includes low Mach, variable density turbulent flow. The ongoing objective for Nalu is to facilitate partnerships with external organizations in order to extend code capability and knowledge; however, it is not intended to support routine CFD analysis. The targeted usage of this module is for non-NW applications that support work-for-others in the multiphysics energy sector.

  6. High temperature solid lubricant materials for heavy duty and advanced heat engines

    SciTech Connect

    DellaCorte, C.; Wood, J.C.

    1994-10-01

    Advanced engine designs incorporate higher mechanical and thermal loading to achieve efficiency improvements. This approach often leads to higher operating temperatures of critical sliding elements (e.g. piston ring/cylinder wall contacts and valve guides) which compromise the use of conventional and even advanced synthetic liquid lubricants. For these applications solid lubricants must be considered. Several novel solid lubricant composites and coatings designated PS/PM200 have been employed to dry and marginally oil lubricated contacts in advanced heat engines. These applications include cylinder kits of heavy duty diesels, and high temperature sterling engines, sidewall seals of rotary engines and various exhaust valve and exhaust component applications. The following paper describes the tribological and thermophysical properties of these tribomaterials and reviews the results of applying them to engine applications. Other potential tribological materials and applications are also discussed with particular emphasis to heavy duty and advanced heat engines.

  7. Methods of staining target chromosomal DNA employing high complexity nucleic acid probes

    DOEpatents

    Gray, Joe W.; Pinkel, Daniel; Kallioniemi, Ol'li-Pekka; Kallioniemi, Anne; Sakamoto, Masaru

    2006-10-03

    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML), retinoblastoma, ovarian and uterine cancers, and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.

  8. Chromosome-specific staining to detect genetic rearrangements associated with chromosome 3 and/or chromosome 17

    DOEpatents

    Gray, Joe W.; Pinkel, Daniel; Kallioniemi, Olli-Pekka; Kallioniemi, Anne; Sakamoto, Masaru

    2008-09-09

    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML), retinoblastoma, ovarian and uterine cancers, and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.

  9. Chromosome-Specific Staining To Detect Genetic Rearrangements Associated With Chromosome 3 And/Or Chromosone 17

    DOEpatents

    Gray; Joe W.; Pinkel; Daniel; Kallioniemi; Olli-Pekka; Kallioniemi; Anne; Sakamoto; Masaru

    2002-02-05

    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML), retinoblastoma, ovarian and uterine cancers, and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.

  10. Apparatus for insulating windows and the like

    DOEpatents

    Mitchell, Robert A.

    1984-01-01

    Apparatus for insulating window openings through walls and the like includes a thermal shutter, a rail for mounting the shutter adjacent to the window opening and a coupling for connecting the shutter to the rail. The thermal shutter includes an insulated panel adhered to frame members which surround the periphery of the panel. The frame members include a hard portion for providing the frame and a soft portion for providing a seal with that portion of the wall adjacent to the periphery of the opening. The coupling means is preferably integral with the attachment rail. According to a preferred embodiment, the coupling means includes a continuous hinge of reduced thickness. The thermal shutter can be permanently attached, hinged, bi-folded, or sliding with respect to the window and wall. A distribution method is to market the apparatus in "kit" form.

  11. Fighting Ebola with novel spore decontamination technologies for the military

    DOE PAGES [OSTI]

    Doona, Christopher J.; Feeherry, Florence E.; Kustin, Kenneth; Olinger, Gene G.; Setlow, Peter; Malkin, Alexander J.; Leighton, Terrance

    2015-08-12

    Recently, global public health organizations such as Doctors without Borders (MSF), the World Health Organization (WHO), Public Health Canada, National Institutes of Health (NIH), and the U.S. government developed and deployed Field Decontamination Kits (FDKs), a novel, lightweight, compact, reusable decontamination technology to sterilize Ebola-contaminated medical devices at remote clinical sites lacking infra-structure in crisis-stricken regions of West Africa (medical waste materials are placed in bags and burned). Here, the basis for effectuating sterilization with FDKs is chlorine dioxide (ClO2) produced from a patented invention developed by researchers at the US Army Natick Soldier RD&E Center (NSRDEC) and commercialized asmore » a dry mixed-chemical for bacterial spore decontamination.« less

  12. Reference Model MHK Turbine Array Optimization Study within a Generic River System.

    SciTech Connect

    Johnson, Erick; Barco Mugg, Janet; James, Scott; Roberts, Jesse D.

    2011-12-01

    Increasing interest in marine hydrokinetic (MHK) energy has spurred to significant research on optimal placement of emerging technologies to maximize energy conversion and minimize potential effects on the environment. However, these devices will be deployed as an array in order to reduce the cost of energy and little work has been done to understand the impact these arrays will have on the flow dynamics, sediment-bed transport and benthic habitats and how best to optimize these arrays for both performance and environmental considerations. An %22MHK-friendly%22 routine has been developed and implemented by Sandia National Laboratories (SNL) into the flow, sediment dynamics and water-quality code, SNL-EFDC. This routine has been verified and validated against three separate sets of experimental data. With SNL-EFDC, water quality and array optimization studies can be carried out to optimize an MHK array in a resource and study its effects on the environment. The present study examines the effect streamwise and spanwise spacing has on the array performance. Various hypothetical MHK array configurations are simulated within a trapezoidal river channel. Results show a non-linear increase in array-power efficiency as turbine spacing is increased in each direction, which matches the trends seen experimentally. While the sediment transport routines were not used in these simulations, the flow acceleration seen around the MHK arrays has the potential to significantly affect the sediment transport characteristics and benthic habitat of a resource. Evaluation Only. Created with Aspose.Pdf.Kit. Copyright 2002-2011 Aspose Pty Ltd Evaluation Only. Created with Aspose.Pdf.Kit. Copyright 2002-2011 Aspose Pty Ltd

  13. 2007 Wholesale Power Rate Case Final Proposal : Risk Analysis Study.

    SciTech Connect

    United States. Bonneville Power Administration.

    2006-07-01

    BPA's operating environment is filled with numerous uncertainties, and thus the rate-setting process must take into account a wide spectrum of risks. The objective of the Risk Analysis is to identify, model, and analyze the impacts that key risks have on BPA's net revenue (total revenues less total expenses). This is carried out in two distinct steps: a risk analysis step, in which the distributions, or profiles, of operating and non operating risks are defined, and a risk mitigation step, in which different rate tools are tested to assess their ability to recover BPA's costs in the face of this uncertainty. Two statistical models are used in the risk analysis step for this rate proposal, the Risk Analysis Model (RiskMod), and the Non-Operating Risk Model (NORM), while a third model, the ToolKit, is used to test the effectiveness of rate tools options in the risk mitigation step. RiskMod is discussed in Sections 2.1 through 2.4, the NORM is discussed in Section 2.5, and the ToolKit is discussed in Section 3. The models function together so that BPA can develop rates that cover all of its costs and provide a high probability of making its Treasury payments on time and in full during the rate period. By law, BPA's payments to Treasury are the lowest priority for revenue application, meaning that payments to Treasury are the first to be missed if financial reserves are insufficient to pay all bills on time. For this reason, BPA measures its potential for recovering costs in terms of probability of being able to make Treasury payments on time (also known as Treasury Payment Probability or TPP).

  14. CALiPER Exploratory Study: Recessed Troffer Lighting

    SciTech Connect

    Miller, Naomi J.; Royer, Michael P.; Poplawski, Michael E.

    2013-04-28

    This report describes an exploration of troffer lighting as used in office and classroom spaces, which was conducted by the CALiPER program. Twenty-four pairs of 2×2 and 2×4 troffers were procured anonymously, documented, tested for photometric and electrical performance, and installed in a mockup office space in Portland, Oregon. Three of the pairs were T8 fluorescent benchmark products, 12 were dedicated LED troffers, five were fluorescent troffers modified for LED lamps (sometimes referred to as "tubes"), and another four troffers were modified with LED retrofit kits. The modifications were performed by a commercial electrical contractor, following the instructions provided by the retrofit lamp or kit manufacturer. Once installed in the mockup facility, the converted luminaires were examined by a NRTL (Nationally Recognized Testing Laboratory) safety expert, who provided feedback on safety issues. In September 2012, a group of lighting designers, engineers, and facility managers were brought in to observe the LED luminaires in comparison to fluorescent benchmarks. This report documents performance in measures that go beyond illuminance values or luminaire efficacy. Dedicated LED troffers are ready to compete with fluorescent troffers in terms of efficacy (lumens per watt), and in many lighting quality issues such as glare, light distribution, visual appearance, and color quality. That is not to say that each one is stellar, but each one tested in this CALiPER study bested the fluorescent benchmarks in terms of efficacy, and almost all were rated highly in several categories -- only one luminaire of twelve performed consistently poorly.

  15. Hydrogen-Enhanced Natural Gas Vehicle Program

    SciTech Connect

    Hyde, Dan; Collier, Kirk

    2009-01-22

    The project objective is to demonstrate the viability of HCNG fuel (30 to 50% hydrogen by volume and the remainder natural gas) to reduce emissions from light-duty on-road vehicles with no loss in performance or efficiency. The City of Las Vegas has an interest in alternative fuels and already has an existing hydrogen refueling station. Collier Technologies Inc (CT) supplied the latest design retrofit kits capable of converting nine compressed natural gas (CNG) fueled, light-duty vehicles powered by the Ford 5.4L Triton engine. CT installed the kits on the first two vehicles in Las Vegas, trained personnel at the City of Las Vegas (the City) to perform the additional seven retrofits, and developed materials for allowing other entities to perform these retrofits as well. These vehicles were used in normal service by the City while driver impressions, reliability, fuel efficiency and emissions were documented for a minimum of one year after conversion. This project has shown the efficacy of operating vehicles originally designed to operate on compressed natural gas with HCNG fuel incorporating large quantities of exhaust gas recirculation (EGR). There were no safety issues experienced with these vehicles. The only maintenance issue in the project was some rough idling due to problems with the EGR valve and piping parts. Once the rough idling was corrected no further maintenance issues with these vehicles were experienced. Fuel economy data showed no significant changes after conversion even with the added power provided by the superchargers that were part of the conversions. Driver feedback for the conversions was very favorable. The additional power provided by the HCNG vehicles was greatly appreciated, especially in traffic. The drivability of the HCNG vehicles was considered to be superior by the drivers. Most of the converted vehicles showed zero oxides of nitrogen throughout the life of the project using the State of Nevada emissions station.

  16. Cryogenic experiences during W7-X HTS-current lead tests

    SciTech Connect

    Richter, Thomas; Lietzow, Ralph

    2014-01-29

    The Karlsruhe Institute of Technology (KIT) was responsible for design, production and test of the High Temperature Superconductor (HTS) current leads (CL) for the stellerator Wendelstein 7-X (W7-X). 16 current leads were delivered. Detailed prototype tests as well as the final acceptance tests were performed at KIT, using a dedicated test cryostat assembled beside and connected to the main vacuum vessel of the TOSKA facility. A unique feature is the upside down orientation of the current leads due to the location of the power supplies in the basement of the experimental area of W7-X. The HTS-CL consists of three main parts: the cold end for the connection to the bus bar at 4.5 K, the HTS part operating in the temperature range from 4.5 K to 65 K and a copper heat exchanger (HEX) in the temperature range from 65 K to room temperature, which is cooled with 50 K helium. Therefore in TOSKA it is possible to cool test specimens simultaneously with helium at two different temperature levels. The current lead tests included different scenarios with currents up to 18.2 kA. In total, 10 cryogenic test campaigns with a total time of about 24 weeks were performed till beginning of 2013. The test facility as well as the 2 kW cryogenic plant of ITEP showed a very good reliability. However, during such a long and complex experimental campaign, one has to deal with failures, technical difficulties and incidents. The paper gives a summary of the test performance comprising the test preparation and operation. This includes the performance and reliability of the refrigerator and the test facility with reference to the process measuring and control system, the data acquisition system, as well as the building infrastructure.

  17. SuperB Progress Report for Physics

    SciTech Connect

    O'Leary, B.; Matias, J.; Ramon, M.; Pous, E.; De Fazio, F.; Palano, A.; Eigen, G.; Asgeirsson, D.; Cheng, C.H.; Chivukula, A.; Echenard, B.; Hitlin, D.G.; Porter, F.; Rakitin, A.; Heinemeyer, S.; McElrath, B.; Andreassen, R.; Meadows, B.; Sokoloff, M.; Blanke, M.; Lesiak, T.; /Cracow, INP /DESY /Zurich, ETH /INFN, Ferrara /Frascati /INFN, Genoa /Glasgow U. /Indiana U. /Mainz U., Inst. Phys. /Karlsruhe, Inst. Technol. /KEK, Tsukuba /LBL, Berkeley /UC, Berkeley /Lisbon, IST /Ljubljana U. /Madrid, Autonoma U. /Maryland U. /MIT /INFN, Milan /McGill U. /Munich, Tech. U. /Notre Dame U. /PNL, Richland /INFN, Padua /Paris U., VI-VII /Orsay, LAL /Orsay, LPT /INFN, Pavia /INFN, Perugia /INFN, Pisa /Queen Mary, U. of London /Regensburg U. /Republica U., Montevideo /Frascati /INFN, Rome /INFN, Rome /INFN, Rome /Rutherford /Sassari U. /Siegen U. /SLAC /Southern Methodist U. /Tel Aviv U. /Tohoku U. /INFN, Turin /INFN, Trieste /Uppsala U. /Valencia U., IFIC /Victoria U. /Wayne State U. /Wisconsin U., Madison

    2012-02-14

    -constraints of the unitarity triangle through multiple measurements of all angles and sides. This report extends and updates the studies presented in both the SuperB Conceptual Design Report in 2007 and the Proceedings of SuperB Workshop VI in Valencia in 2008. Together, these three documents detail the Physics case of the SuperB Project.

  18. Final report on SNAC 11

    SciTech Connect

    Huber, Patrick

    2013-06-26

    This report details how the $5,000 DOE grant to support the workshop titled “Sterile Neutrinos at the Crossroads” (or SNAC11) was allocated and spent. The SNAC11 workshop covered three days during which there were 28 talks, multiple discussion sessions, a poster session with 9 posters delivered, and an impromptu public lecture on the OPERA superluminal neutrino result by the former project manager of OPERA (this was the first official OPERA talk on the subject in North America). The workshop scientific agenda can be viewed at http://www.cpe.vt.edu/snac/program.html. Emerging out of the workshop discussions, was the idea to write a comprehensive white paper describing the current state of the light sterile neutrino. This effort soon became an international collaboration. The final document, titled “Light Sterile Neutrinos: A White Paper” has nearly 200 authors, is 267 pages long, and cites 730 unique references. It has been posted the preprint archive as arXiv:1204.5379 [hep-ph]. Workshop local organizing committee co-chairs, Patrick Huber and Jonathan Link, are the white paper’s head editors. The white paper’s sections and section editors are as follows: 1. Theory and Motivation (Gabriela Barenboim, Valencia and Werner Rodejohann, MPI Heidelberg) 2. Astrophysical Evidence (Kev Abazajian, UC Irvine and Yvonne Wong, Aachen) 3. Evidence from Oscillation Experiments (Joachim Kopp, FNAL and Bill Louis, LANL) 4. Global Picture (Thierry Lasserre, CEA Saclay and Thomas Schwetz, MPI Heidelberg) 5. Requirements for Future Measurements (Bonnie Fleming, Yale and Joe Formaggio, MIT) 6. Appendix: Possible Future Experiments (Patrick Huber, Virginia Tech and Jon Link, Virginia Tech) In all 56 people participated in the workshop, of these 11 were young scientists. The workshop was covered in a feature article in Science (Science, 334, (2011), 304-306.). The DOE award was spent, as budgeted, as contractual services to VT CPE, which is the unit within the University

  19. Exposure to mercury among Spanish preschool children: Trend from birth to age four

    SciTech Connect

    Llop, Sabrina; Murcia, Mario; Aguinagalde, Xabier; Vioque, Jesus; Rebagliato, Marisa; Iñiguez, Carmen; Lopez-Espinosa, Maria-Jose; Amurrio, Ascensión; María Navarrete-Muñoz, Eva; and others

    2014-07-15

    The purpose of this study is to describe the total hair mercury concentrations and their determinants in preschool Spanish children, as well as to explore the trend in mercury exposure from birth to the age four. This evolution has been scarcely studied in other birth cohort studies. The study population was 580 four year old children participating in the INMA (i.e. Childhood and Environment) birth cohort study in Valencia (2008–2009). Total mercury concentration at age four was measured in hair samples by atomic absorption spectrometry. Fish consumption and other covariates were obtained by questionnaire. Multivariate linear regression models were conducted in order to explore the association between mercury exposure and fish consumption, socio-demographic characteristics and prenatal exposure to mercury. The geometric mean was 1.10 µg/g (95%CI: 1.02, 1.19). Nineteen percent of children had mercury concentrations above the equivalent to the Provisional Tolerable Weekly Intake proposed by WHO. Mercury concentration was associated with increasing maternal age, fish consumption and cord blood mercury levels, as well as decreasing parity. Children whose mothers worked had higher mercury levels than those with non working mothers. Swordfish, lean fish and canned fish were the fish categories most associated with hair mercury concentrations. We observed a decreasing trend in mercury concentrations between birth and age four. In conclusion, the children participating in this study had high hair mercury concentrations compared to reported studies on children from other European countries and similar to other countries with high fish consumption. The INMA study design allows the evaluation of the exposure to mercury longitudinally and enables this information to be used for biomonitoring purposes and dietary recommendations. - Highlights: • The geometric mean of hair Hg concentrations was 1.10 µg/g. • 19% of children had Hg concentrations above the RfD proposed by

  20. Technical Feasibility Assessment of LED Roadway Lighting on the Golden Gate Bridge

    SciTech Connect

    Tuenge, Jason R.

    2012-09-01

    Subsequent to preliminary investigations by the Golden Gate Bridge Highway & Transportation District (GGB), in coordination with Pacific Gas & Electric (PG&E), the GATEWAY Demonstration program was asked to evaluate the technical feasibility of replacing existing roadway lighting on the bridge with products utilizing LED technology. GGB and PG&E also indicated interest in induction (i.e., electrodeless fluorescent) technology, since both light source types feature rated lifetimes significantly exceeding those of the existing high-pressure sodium (HPS) and low-pressure sodium (LPS) products. The goal of the study was to identify any solutions which would reduce energy use and maintenance without compromising the quantity or quality of existing illumination. Products used for roadway lighting on the historic bridge must be installed within the existing amber-lensed shoebox-style luminaire housings. It was determined that induction technology does not appear to represent a viable alternative for the roadway luminaires in this application; any energy savings would be attributable to a reduction in light levels. Although no suitable LED retrofit kits were identified for installation within existing luminaire housings, several complete LED luminaires were found to offer energy savings of 6-18%, suggesting custom LED retrofit kits could be developed to match or exceed the performance of the existing shoeboxes. Luminaires utilizing ceramic metal halide (CMH) were also evaluated, and some were found to offer 28% energy savings, but these products might actually increase maintenance due to the shorter rated lamp life. Plasma technology was evaluated, as well, but no suitable products were identified. Analysis provided in this report was completed in May 2012. Although LED technologies are expected to become increasingly viable over time, and product mock-ups may reveal near-term solutions, some options not currently considered by GGB may ultimately merit evaluation. For

  1. FIELD SCREENING FOR HALOGENATED VOLATILE ORGANIC COMPOUNDS

    SciTech Connect

    John F. Schabron; Joseph F. Rovani, Jr.; Theresa M. Bomstad

    2003-07-01

    Western Research Institute (WRI) is continuing work toward the development of new screening methodology and a test kit to measure halogenated volatile organic compounds (VOCs) in the field. Heated diode and corona discharge sensors are commonly used to detect leaks of refrigerants from air conditioners, freezers, and refrigerators. They are both selective to the presence of halogens. In prior work, the devices were tested for response to carbon tetrachloride, heptane, toluene, and water vapors. In the current work, sensor response was evaluated with sixteen halogenated VOCs relative to carbon tetrachloride. The results show that the response of the various chlorinated VOCs is within an order of magnitude of the response to carbon tetrachloride for each of the sensors. Thus, for field screening a single response factor can be used. Both types of leak detectors are being further modified to provide an on-board LCD signal readout, which is related to VOC concentration. The units will be fully portable and will operate with 115-V line or battery power. Signal background, noise level, and response data on the Bacharach heated diode detector and the TIF corona discharge detector show that when the response curves are plotted against the log of concentration, the plot is linear to the upper limit for the particular unit, with some curvature at lower levels. When response is plotted directly against concentration, the response is linear at the low end and is curved at the high end. The dynamic ranges for carbon tetrachloride of the two devices from the lower detection limit (S/N=2) to signal saturation are 4-850 vapor parts per million (vppm) for the corona discharge unit and 0.01-70 vppm for the heated diode unit. Additional circuit modifications are being made to lower the detection limit and increase the dynamic response range of the corona discharge unit. The results indicate that both devices show potential utility for future analytical method development work toward

  2. Effects of heavy metals on biomarkers for oxidative stress in Griffon vulture (Gyps fulvus)

    SciTech Connect

    Espn, Silvia; Martnez-Lpez, Emma; Jimnez, Pedro; Mara-Mojica, Pedro; Garca-Fernndez, Antonio J.

    2014-02-01

    Metals are involved in the formation of reactive oxygen species (ROS) which may result in metal-related oxidative stress that can lead to oxidative damage to lipids, DNA and proteins. It is necessary to understand the mechanisms of metal toxicity in wild birds, and the concentrations that cause effects on oxidative stress biomarkers. The aim of this study is to assess the concentrations of lead (Pb), cadmium (Cd), mercury (Hg), copper (Cu) and zinc (Zn) with regards to oxidative stress in blood samples of 66 Griffon vultures (Gyps fulvus) from two areas of the Autonomous Community of Valencia (East of Spain). The two study areas (Alcoy n=36 and Cinctorres n=30) were selected as random locations of interest that had not yet been studied, and are feeding stations where supplementary food, mainly of pork origin, is provided for vultures. Given that the two study areas are not considered polluted sites, we expected to find low metal concentrations. However, there are no known threshold concentrations at which metals can affect antioxidant systems, and low metal levels may have an effect on antioxidant biomolecules. In this study, since sampling was done at the beginning of the hunting season, the low Pb levels found in most Griffon vultures from Alcoy and Cinctorres (median=12.37 and 16.26 ?g/dl, respectively) are suggestive of background levels usually found in vultures that feed on pork carcasses all year round. The ingestion of game meat with bullet fragments in carcasses or with Pb shots embedded in the flesh could be the cause of the high blood Pb concentrations found in three vultures from Cinctorres (83, 290 and 362 ?g/dl). Griffon vultures feeding in Cinctorres had enhanced CAT and GST activities and tGSH concentrations, which may be interpreted as protective response against the higher TBARS levels. This study provides threshold concentrations at which metals affect antioxidant system derived from 66 samples of Griffon vulture. Blood Cd concentrations greater

  3. Methylocystis strain SB2 materials and methods

    DOEpatents

    Semrau, Jeremy D; Gallagher, Warren; Yoon, Sukhwan; Im, Jeongdae; DiSpririto, Alan A; Lee, Sung-Woo; Hartsel, Scott; McEllistrem, Marcus T

    2014-01-14

    The present disclosures provides isolated or purified compounds, each of which bind to a metal atom. Generally, the compounds are small in size (e.g., molecular weight of less than about 1 kDa) and peptidic in nature, inasmuch as the compounds comprise amino acids. In some embodiments, the compound comprises a structure of Formula I; M.sub.1-P.sub.1-M.sub.2-P.sub.2 wherein each of P.sub.1 and P.sub.2 is a peptide comprising at least two amino acids, M.sub.1 is a first metal binding moiety comprising a substituted imidazolone ring, M.sub.2 is a second metal binding moiety comprising a substituted oxazolone ring, and wherein M.sub.1 and M.sub.2 bind to a single metal atom. Also provided are related complexes, conjugates, cells which synthesize the compounds of the present disclosures, substantially homogenous cultures thereof, kits and compositions, and methods of making or using the materials of the present disclosures.

  4. High explosive spot test analyses of samples from Operable Unit (OU) 1111

    SciTech Connect

    McRae, D.; Haywood, W.; Powell, J.; Harris, B.

    1995-01-01

    A preliminary evaluation has been completed of environmental contaminants at selected sites within the Group DX-10 (formally Group M-7) area. Soil samples taken from specific locations at this detonator facility were analyzed for harmful metals and screened for explosives. A sanitary outflow, a burn pit, a pentaerythritol tetranitrate (PETN) production outflow field, an active firing chamber, an inactive firing chamber, and a leach field were sampled. Energy dispersive x-ray fluorescence (EDXRF) was used to obtain semi-quantitative concentrations of metals in the soil. Two field spot-test kits for explosives were used to assess the presence of energetic materials in the soil and in items found at the areas tested. PETN is the major explosive in detonators manufactured and destroyed at Los Alamos. No measurable amounts of PETN or other explosives were detected in the soil, but items taken from the burn area and a high-energy explosive (HE)/chemical sump were contaminated. The concentrations of lead, mercury, and uranium are given.

  5. System Advisor Model, SAM 2014.1.14: General Description

    SciTech Connect

    Blair, N.; Dobos, A. P.; Freeman, J.; Neises, T.; Wagner, M.; Ferguson, T.; Gilman, P.; Janzou, S.

    2014-02-01

    This document describes the capabilities of the U.S. Department of Energy and National Renewable Energy Laboratory's System Advisor Model (SAM), Version 2013.9.20, released on September 9, 2013. SAM is a computer model that calculates performance and financial metrics of renewable energy systems. Project developers, policy makers, equipment manufacturers, and researchers use graphs and tables of SAM results in the process of evaluating financial, technology, and incentive options for renewable energy projects. SAM simulates the performance of photovoltaic, concentrating solar power, solar water heating, wind, geothermal, biomass, and conventional power systems. The financial model can represent financial structures for projects that either buy and sell electricity at retail rates (residential and commercial) or sell electricity at a price determined in a power purchase agreement (utility). SAM's advanced simulation options facilitate parametric and sensitivity analyses, and statistical analysis capabilities are available for Monte Carlo simulation and weather variability (P50/P90) studies. SAM can also read input variables from Microsoft Excel worksheets. For software developers, the SAM software development kit (SDK) makes it possible to use SAM simulation modules in their applications written in C/C++, C#, Java, Python, and MATLAB. NREL provides both SAM and the SDK as free downloads at http://sam.nrel.gov. Technical support and more information about the software are available on the website.

  6. A wide variety of injection molding technologies is now applicable to small series and mass production

    SciTech Connect

    Blo, P., E-mail: bloss@kuz-leipzig.de, E-mail: juettner@kuz-leipzig.de, E-mail: jacob@kuz-leipzig.de, E-mail: loeser@kuz-leipzig.de, E-mail: michaelis@kuz-leipzig.de, E-mail: krajewsky@kuz-leipzig.de; Jttner, G., E-mail: bloss@kuz-leipzig.de, E-mail: juettner@kuz-leipzig.de, E-mail: jacob@kuz-leipzig.de, E-mail: loeser@kuz-leipzig.de, E-mail: michaelis@kuz-leipzig.de, E-mail: krajewsky@kuz-leipzig.de; Jacob, S., E-mail: bloss@kuz-leipzig.de, E-mail: juettner@kuz-leipzig.de, E-mail: jacob@kuz-leipzig.de, E-mail: loeser@kuz-leipzig.de, E-mail: michaelis@kuz-leipzig.de, E-mail: krajewsky@kuz-leipzig.de; Lser, C., E-mail: bloss@kuz-leipzig.de, E-mail: juettner@kuz-leipzig.de, E-mail: jacob@kuz-leipzig.de, E-mail: loeser@kuz-leipzig.de, E-mail: michaelis@kuz-leipzig.de, E-mail: krajewsky@kuz-leipzig.de; Michaelis, J., E-mail: bloss@kuz-leipzig.de, E-mail: juettner@kuz-leipzig.de, E-mail: jacob@kuz-leipzig.de, E-mail: loeser@kuz-leipzig.de, E-mail: michaelis@kuz-leipzig.de, E-mail: krajewsky@kuz-leipzig.de; Krajewsky, P., E-mail: bloss@kuz-leipzig.de, E-mail: juettner@kuz-leipzig.de, E-mail: jacob@kuz-leipzig.de, E-mail: loeser@kuz-leipzig.de, E-mail: michaelis@kuz-leipzig.de, E-mail: krajewsky@kuz-leipzig.de [Kunststoff-Zentrum in Leipzig gGmbH (KuZ), Leipzig (Germany)

    2014-05-15

    Micro plastic parts open new fields for application, e. g., to electronics, sensor technologies, optics, and medical engineering. Before micro parts can go to mass production, there is a strong need of having the possibility for testing different designs and materials including material combinations. Hence, flexible individual technical and technological solutions for processing are necessary. To manufacture high quality micro parts, a micro injection moulding machine named formicaPlast based on a two-step plunger injection technology was developed. Resulting from its design, the residence time and the accuracy problems for managing small shot volumes with reproducible high accuracy are uncompromisingly solved. Due to their simple geometry possessing smooth transitions and non adherent inner surfaces, the plunger units allow to process 'all' thermoplastics from polyolefines to high performance polymers, optical clear polymers, thermally sensitive bioresorbables, highly filled systems (the so-called powder injection molding PIM), and liquid silicon rubber (LSR, here with a special kit). The applied platform strategy in the 1K and 2K version allows integrating automation for assembling, handling and packaging. A perpendicular arrangement allows encapsulation of inserts, also partially, and integration of this machine into process chains. Considering a wide variety of different parts consisting of different materials, the high potential of the technology is demonstrated. Based on challenging industrial parts from electronic applications (2K micro MID and bump mat, where both are highly structured parts), the technological solutions are presented in more detail.

  7. Detection and isolation of nucleic acid sequences using competitive hybridization probes

    DOEpatents

    Lucas, Joe N.; Straume, Tore; Bogen, Kenneth T.

    1997-01-01

    A method for detecting a target nucleic acid sequence in a sample is provided using hybridization probes which competitively hybridize to a target nucleic acid. According to the method, a target nucleic acid sequence is hybridized to first and second hybridization probes which are complementary to overlapping portions of the target nucleic acid sequence, the first hybridization probe including a first complexing agent capable of forming a binding pair with a second complexing agent and the second hybridization probe including a detectable marker. The first complexing agent attached to the first hybridization probe is contacted with a second complexing agent, the second complexing agent being attached to a solid support such that when the first and second complexing agents are attached, target nucleic acid sequences hybridized to the first hybridization probe become immobilized on to the solid support. The immobilized target nucleic acids are then separated and detected by detecting the detectable marker attached to the second hybridization probe. A kit for performing the method is also provided.

  8. Detection and isolation of nucleic acid sequences using competitive hybridization probes

    DOEpatents

    Lucas, J.N.; Straume, T.; Bogen, K.T.

    1997-04-01

    A method for detecting a target nucleic acid sequence in a sample is provided using hybridization probes which competitively hybridize to a target nucleic acid. According to the method, a target nucleic acid sequence is hybridized to first and second hybridization probes which are complementary to overlapping portions of the target nucleic acid sequence, the first hybridization probe including a first complexing agent capable of forming a binding pair with a second complexing agent and the second hybridization probe including a detectable marker. The first complexing agent attached to the first hybridization probe is contacted with a second complexing agent, the second complexing agent being attached to a solid support such that when the first and second complexing agents are attached, target nucleic acid sequences hybridized to the first hybridization probe become immobilized on to the solid support. The immobilized target nucleic acids are then separated and detected by detecting the detectable marker attached to the second hybridization probe. A kit for performing the method is also provided. 7 figs.

  9. Bioelectrochemical Immunoassay of Polychlorinated Biphenyl

    SciTech Connect

    Lin, Ying-Ying; Liu, Guodong; Wai, Chien M.; Lin, Yuehe

    2008-04-01

    A simple, rapid, and highly sensitive bioelectrochemical immunoassay method based on magnetic beads (MBs) and disposable screen-printed electrodes (SPE) has been developed to detect polychlorinated biphenyls (PCBs). The principle of this bioassay is based on a direct competitive enzyme-linked immunosorbent assay using PCB-antibody-coated MBs and horseradish peroxidase (HRP)-labeled PCB (HRP-PCB). A magnetic process platform was used to mix and shake the samples during the immunoreactions and to separate free and unbound reagents after the liquid-phase competitive immunoreactions among PCB-antibody-coated MBs, PCB analyte, and HRP-PCB. After a complete immunoassay, the HRP tracers attached to MBs were transferred to a substrate solution containing o-aminophenol and hydrogen peroxide for electrochemical detection. The different parameters, including the amount of HRP-PCB conjugates, immunoreaction time, and the concentration of substrate that governs the analytical performance of the immunoassay have been studied in detail and optimized. The detection limit of 5 pg mL-1 was obtained under optimum experimental conditions. The performance of this bioelectrochemical immunoassay was successfully evaluated with untreated river water spiked with PCBs, and the results were validated by commercial PCB enzyme-linked immunosorbent assay kit, indicating that this convenient and sensitive technique offers great promise for decentralized environmental application and trace PCBs monitoring.

  10. Guide for the preparation of applications for licenses for the use of radioactive materials in leak-testing services. Draft regulatory guide and value/impact statement

    SciTech Connect

    Not Available

    1985-06-01

    The purpose of this regulatory guide is to provide assistance to applicants and licensees in preparing applications for new licenses, license amendments, and license renewals. This guide describes the information the NRC staff needs to evaluate an application for a license for the use of radioactive material in performing certain commercial services for NRC or Agreement State licensees. The services covered by this guide are leak-testing sealed sources, analyzing leak-test samples, and supplying preregistered leak-test kits. This regulatory guide is intended to provide the applicant or licensee with information that will enable him to understand specific regulatory requirements and licensing policies as they apply to his services. The information in this guide is not a substitute for training in radiation safety. After the applicant is issued a license, he must conduct his program in accordance with (1) the statements, representations, and procedures contained in his application, (2) the terms and conditions of the license, and (3) the NRC's regulations. The information provided in the application should be clear, specific, and accurate.

  11. Remote I/O : fast access to distant storage.

    SciTech Connect

    Foster, I.; Kohr, D., Jr.; Krishnaiyer, R.; Mogill, J.

    1997-12-17

    As high-speed networks make it easier to use distributed resources, it becomes increasingly common that applications and their data are not colocated. Users have traditionally addressed this problem by manually staging data to and from remote computers. We argue instead for a new remote I/O paradigm in which programs use familiar parallel I/O interfaces to access remote file systems. In addition to simplifying remote execution, remote I/O can improve performance relative to staging by overlapping computation and data transfer or by reducing communication requirements. However, remote I/O also introduces new technical challenges in the areas of portability, performance, and integration with distributed computing systems. We propose techniques designed to address these challenges and describe a remote I/O library called RIO that we have developed to evaluate the effectiveness of these techniques. RIO addresses issues of portability by adopting the quasi-standard MPI-IO interface and by defining a RIO device and RIO server within the ADIO abstract I/O device architecture. It addresses performance issues by providing traditional I/O optimizations such as asynchronous operations and through implementation techniques such as buffering and message forwarding to off load communication overheads. RIO uses the Nexus communication library to obtain access to configuration and security mechanisms provided by the Globus wide area computing tool kit. Microbenchmarks and application experiments demonstrate that our techniques achieve acceptable performance in most situations and can improve turnaround time relative to staging.

  12. Chromosome-specific staining to detect genetic rearrangements associated with chromosome 3 and/or chromosome 17

    DOEpatents

    Gray, Joe W.; Pinkel, Daniel; Kallioniemi, Olli-Pekka; Kallioniemi, Anne; Sakamoto, Masaru

    2009-10-06

    Methods and compositions for staining based upon nucleic acid sequence that employ nudeic nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML), retinoblastoma, ovarian and uterine cancers, and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.

  13. Dakota, a multilevel parallel object-oriented framework for design optimization, parameter estimation, uncertainty quantification, and sensitivity analysis :

    SciTech Connect

    Adams, Brian M.; Ebeida, Mohamed Salah; Eldred, Michael S.; Jakeman, John Davis; Swiler, Laura Painton; Stephens, John Adam; Vigil, Dena M.; Wildey, Timothy Michael; Bohnhoff, William J.; Eddy, John P.; Hu, Kenneth T.; Dalbey, Keith R.; Bauman, Lara E; Hough, Patricia Diane

    2014-05-01

    The Dakota (Design Analysis Kit for Optimization and Terascale Applications) toolkit provides a exible and extensible interface between simulation codes and iterative analysis methods. Dakota contains algorithms for optimization with gradient and nongradient-based methods; uncertainty quanti cation with sampling, reliability, and stochastic expansion methods; parameter estimation with nonlinear least squares methods; and sensitivity/variance analysis with design of experiments and parameter study methods. These capabilities may be used on their own or as components within advanced strategies such as surrogate-based optimization, mixed integer nonlinear programming, or optimization under uncertainty. By employing object-oriented design to implement abstractions of the key components required for iterative systems analyses, the Dakota toolkit provides a exible and extensible problem-solving environment for design and performance analysis of computational models on high performance computers. This report serves as a user's manual for the Dakota software and provides capability overviews and procedures for software execution, as well as a variety of example studies.

  14. Secure Control Systems for the Energy Sector

    SciTech Connect

    Smith, Rhett; Campbell, Jack; Hadley, Mark

    2012-03-31

    Schweitzer Engineering Laboratories (SEL) will conduct the Hallmark Project to address the need to reduce the risk of energy disruptions because of cyber incidents on control systems. The goals is to develop solutions that can be both applied to existing control systems and designed into new control systems to add the security measures needed to mitigate energy network vulnerabilities. The scope of the Hallmark Project contains four primary elements: 1. Technology transfer of the Secure Supervisory Control and Data Acquisition (SCADA) Communications Protocol (SSCP) from Pacific Northwest National Laboratories (PNNL) to Schweitzer Engineering Laboratories (SEL). The project shall use this technology to develop a Federal Information Processing Standard (FIPS) 140-2 compliant original equipment manufacturer (OEM) module to be called a Cryptographic Daughter Card (CDC) with the ability to directly connect to any PC enabling that computer to securely communicate across serial to field devices. Validate the OEM capabilities with another vendor. 2. Development of a Link Authenticator Module (LAM) using the FIPS 140-2 validated Secure SCADA Communications Protocol (SSCP) CDC module with a central management software kit. 3. Validation of the CDC and Link Authenticator modules via laboratory and field tests. 4. Creation of documents that record the impact of the Link Authenticator to the operators of control systems and on the control system itself. The information in the documents can assist others with technology deployment and maintenance.

  15. Retrospective evaluation of dosimetric quality for prostate carcinomas treated with 3D conformal, intensity modulated and volumetric modulated arc radiotherapy

    SciTech Connect

    Crowe, Scott B; Kairn, Tanya; Middlebrook, Nigel; Hill, Brendan; Christie, David R H; Knight, Richard T; Kenny, John; Langton, Christian M; Trapp, Jamie V

    2013-12-15

    This study examines and compares the dosimetric quality of radiotherapy treatment plans for prostate carcinoma across a cohort of 163 patients treated across five centres: 83 treated with three-dimensional conformal radiotherapy (3DCRT), 33 treated with intensity modulated radiotherapy (IMRT) and 47 treated with volumetric modulated arc therapy (VMAT). Treatment plan quality was evaluated in terms of target dose homogeneity and organs at risk (OAR), through the use of a set of dose metrics. These included the mean, maximum and minimum doses; the homogeneity and conformity indices for the target volumes; and a selection of dose coverage values that were relevant to each OAR. Statistical significance was evaluated using two-tailed Welch's T-tests. The Monte Carlo DICOM ToolKit software was adapted to permit the evaluation of dose metrics from DICOM data exported from a commercial radiotherapy treatment planning system. The 3DCRT treatment plans offered greater planning target volume dose homogeneity than the other two treatment modalities. The IMRT and VMAT plans offered greater dose reduction in the OAR: with increased compliance with recommended OAR dose constraints, compared to conventional 3DCRT treatments. When compared to each other, IMRT and VMAT did not provide significantly different treatment plan quality for like-sized tumour volumes. This study indicates that IMRT and VMAT have provided similar dosimetric quality, which is superior to the dosimetric quality achieved with 3DCRT.

  16. DAKOTA : a multilevel parallel object-oriented framework for design optimization, parameter estimation, uncertainty quantification, and sensitivity analysis.

    SciTech Connect

    Eldred, Michael Scott; Vigil, Dena M.; Dalbey, Keith R.; Bohnhoff, William J.; Adams, Brian M.; Swiler, Laura Painton; Lefantzi, Sophia; Hough, Patricia Diane; Eddy, John P.

    2011-12-01

    The DAKOTA (Design Analysis Kit for Optimization and Terascale Applications) toolkit provides a flexible and extensible interface between simulation codes and iterative analysis methods. DAKOTA contains algorithms for optimization with gradient and nongradient-based methods; uncertainty quantification with sampling, reliability, and stochastic expansion methods; parameter estimation with nonlinear least squares methods; and sensitivity/variance analysis with design of experiments and parameter study methods. These capabilities may be used on their own or as components within advanced strategies such as surrogate-based optimization, mixed integer nonlinear programming, or optimization under uncertainty. By employing object-oriented design to implement abstractions of the key components required for iterative systems analyses, the DAKOTA toolkit provides a flexible and extensible problem-solving environment for design and performance analysis of computational models on high performance computers. This report serves as a theoretical manual for selected algorithms implemented within the DAKOTA software. It is not intended as a comprehensive theoretical treatment, since a number of existing texts cover general optimization theory, statistical analysis, and other introductory topics. Rather, this manual is intended to summarize a set of DAKOTA-related research publications in the areas of surrogate-based optimization, uncertainty quantification, and optimization under uncertainty that provide the foundation for many of DAKOTA's iterative analysis capabilities.

  17. Development, sensitivity analysis, and uncertainty quantification of high-fidelity arctic sea ice models.

    SciTech Connect

    Peterson, Kara J.; Bochev, Pavel Blagoveston; Paskaleva, Biliana S.

    2010-09-01

    Arctic sea ice is an important component of the global climate system and due to feedback effects the Arctic ice cover is changing rapidly. Predictive mathematical models are of paramount importance for accurate estimates of the future ice trajectory. However, the sea ice components of Global Climate Models (GCMs) vary significantly in their prediction of the future state of Arctic sea ice and have generally underestimated the rate of decline in minimum sea ice extent seen over the past thirty years. One of the contributing factors to this variability is the sensitivity of the sea ice to model physical parameters. A new sea ice model that has the potential to improve sea ice predictions incorporates an anisotropic elastic-decohesive rheology and dynamics solved using the material-point method (MPM), which combines Lagrangian particles for advection with a background grid for gradient computations. We evaluate the variability of the Los Alamos National Laboratory CICE code and the MPM sea ice code for a single year simulation of the Arctic basin using consistent ocean and atmospheric forcing. Sensitivities of ice volume, ice area, ice extent, root mean square (RMS) ice speed, central Arctic ice thickness, and central Arctic ice speed with respect to ten different dynamic and thermodynamic parameters are evaluated both individually and in combination using the Design Analysis Kit for Optimization and Terascale Applications (DAKOTA). We find similar responses for the two codes and some interesting seasonal variability in the strength of the parameters on the solution.

  18. Data processing unit and power system for the LANL REM instrument package. Final report

    SciTech Connect

    Lockhart, W.

    1994-03-01

    The NEPSTP spacecraft needs highly reliable instrumentation to measure the nuclear reactor health and performance. These reactor measurements are essential for initial on-orbit phase operations and documentation of performance over time. Los Alamos National Laboratory (LANL), under the guidance of W. C. Feldman, principal investigator, has designed the Radiation Environment Monitoring (REM) package to meet these needs. The instrumentation package contains two neutron detectors, one gamma-ray detector, a data processing unit, and an instrument power system. The REM package is an integration of quick turn-around, state of the practice technology for detectors, data processors, and power systems. A significant portion of REM consists of subsystems with flight history. Southwest Research Institute (SwRI) has been tasked by LANL to design support electronics, including the Data Processing Unit (DPU) and Power System for REM. The goal for this project is to use technologies from current programs to speed up and simplify the design process. To meet these design goals, the authors use an open architecture VME bus for the DPU and derivatives of CASSINI power supplies for the instrument power system. To simplify integration and test activities, they incorporate a proven software development strategy and tool kits from outside vendors. The objective of this report is to illustrate easily incorporated system level designs for the DPU, power system and ground support electronics (GSE) in support of the important NEPSTP program.

  19. Solid phase sequencing of double-stranded nucleic acids

    DOEpatents

    Fu, Dong-Jing; Cantor, Charles R.; Koster, Hubert; Smith, Cassandra L.

    2002-01-01

    This invention relates to methods for detecting and sequencing of target double-stranded nucleic acid sequences, to nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probe comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include nucleic acids in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated determination of molecular weights and identification of the target sequence.

  20. Novel Approach to Prepare {sup 99m}Tc-Based Multivalent RGD Peptides

    SciTech Connect

    Shuang Liu

    2012-10-24

    This project presents a novel approach to prepare the {sup 99m}Tc-bridged multivalent RGD (arginine-glycine-aspartate) peptides. This project will focus on fundamentals of {sup 99m}Tc radiochemistry. The main objective of this project is to demonstrate the proof-of-principle for the proposed radiotracers. Once a kit formulation is developed for preparation of the {sup 99m}Tc-bridged multivalent RGD peptides, various tumor-bearing animal models will be used to evaluate their potential for SPECT (single photon-emission computed tomography) imaging of cancer. We have demonstrated that (1) multimerization of cyclic RGD peptides enhances the integrin {alpha}{sub v}{beta}{sub 3} bonding affinity and radiotracer tumor uptake; (2) addition of G{sub 3} or PEG{sub 4} linkers makes it possible for two RGD motifs in 3P-RGD{sub 2} and 3G-RGD{sub 2} to achieve simultaneous integrin {alpha}{sub v}{beta}{sub 3} binding; and (3) multimers are actually bivalent (not multivalent), the presence of extra RGD motifs can enhance the tumor retention time of the radiotracer.