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Sample records for find unedited transcripts

  1. QTR - unedited comments | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    - unedited comments QTR - unedited comments Comments received in response to the DOE-QTR Request For Information (24.47 MB) More Documents & Publications Table of QTR comments in response to Federal Register RFI Table of QTR comments in response to Federal Register RFI QTR table of respondents

  2. Finding

    Office of Legacy Management (LM)

    Finding of No Significant Impact Ground Water Compliance at the Grand Junction UMTRA Project Site (Climax Uranium Millsite) September 1999 U.S. Department of Energy Grand Junction ...

  3. Fall 2005 Meeting of the ASA

    Energy Information Administration (EIA) (indexed site)

    6 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you may find unedited transcripts of EIA's fall 2006 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts. The public meeting took place October 6 and 7,

  4. Fall 2007 ASA Meeting Disclaimer

    Energy Information Administration (EIA) (indexed site)

    7 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts of EIA's fall 2007 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings can be found to the right of the Thursday and Friday transcripts. The public meeting took place October 18 and

  5. Spring 2006 ASA Meeting Disclaimer

    Energy Information Administration (EIA) (indexed site)

    6 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you may find unedited transcripts of EIA's spring 2006 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts. The public meeting took place April 6 and 7,

  6. Spring 2006 ASA Meeting Disclaimer

    Energy Information Administration (EIA) (indexed site)

    7 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts of EIA's spring 2007 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings can be found to the right of the Thursday and Friday transcripts. The public meeting took place April 19 and

  7. Spring 2008 ASA Meeting Disclaimer

    Energy Information Administration (EIA) (indexed site)

    8 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts of EIA's fall 2008 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings can be found to the right of the Thursday and Friday transcripts. The public meeting took place October 23 and

  8. Spring 2008 ASA Meeting Disclaimer

    Energy Information Administration (EIA) (indexed site)

    8 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts of EIA's spring 2008 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings can be found to the right of the Thursday and Friday transcripts. The public meeting took place on April 9,

  9. Spring 2009 ASA Meeting Disclaimer

    Energy Information Administration (EIA) (indexed site)

    9 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts of EIA's spring 2009 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings can be found to the right of the Thursday and Friday transcripts. The public meeting took place on April 2

  10. Untitled Document

    Energy Information Administration (EIA) (indexed site)

    5 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you may find unedited transcripts of EIA's spring 2005 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts, here at:

  11. Find Journals

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    | Department of Energy Find Energy Incentives Quicker and Easier with DSIRE Open Data and Website Find Energy Incentives Quicker and Easier with DSIRE Open Data and Website March 3, 2015 - 4:16pm Addthis The updated Database of State Incentives for Renewables & Efficiency helps homeowners and businesses find incentive programs that can reduce or defray installation or purchase costs of technologies like photovoltaic systems. | Photo by Dennis Schroeder, National Renewable Energy

  12. Find Reports

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Classified reports - contact the SI-RMS Technical Report Collection for an appointment: ... Office 94 Cities of Gold Road Santa Fe, NM 87506 Where can I find Classified Reports? ...

  13. Find Clear

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    p align"left">

    Find Clear Slide Title Duration Status myMail@adobe.com http:Adobe.com More Info>>

  14. Find Books

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Books Find Books The Library Catalog is available to the public, though many eBooks are limited to LANL researchers. Question? 667-5809 Email Library Catalog: Search here for all...

  15. Find Clear

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    p align="left"></p> <p align="left"></p> Find Clear Slide Title Duration Status myMail@adobe.com http://Adobe.com More Info>> <p align="left"></p> <p align="left"></p> <p align="left"></p> Go No Matches Found

  16. Untitled Document

    Energy Information Administration (EIA) (indexed site)

    4 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In an adjacent file you may find an unedited transcript of EIA's Fall 2004 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. The public meeting took place October 28 and 29, 2004 in the Forrestal Building at 1000 Independence Ave., S.W., Washington, D.C. 20585. All of

  17. Find Los Alamos Publications

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Los Alamos Publications Find Los Alamos Publications Some of these resources are Lab-only and will require SSL Portal. Questions? 667-5809 Email Current Lab publications 1663 ...

  18. EIS-0458: Scoping Meeting Transcript

    Office of Energy Efficiency and Renewable Energy (EERE)

    Transcript of the public scoping meeting on the draft EIS for the proposed loan guarantee for the First Solar Topaz Solar Farm Project in San Luis Obispo County, CA.

  19. Transcript for Building America Video

    Energy.gov [DOE]

    Transcript of a video about how DOE’s Building America program is helping to bridge the gap between homes with high energy costs and homes that are healthy, durable, and energy efficient.

  20. RNA-guided transcriptional regulation

    DOEpatents

    Church, George M.; Mali, Prashant G.; Esvelt, Kevin M.

    2016-02-23

    Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.

  1. Find Theses and Dissertations

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Theses and Dissertations Find Theses and Dissertations Most of the theses sources below are publicly accessible. Questions? 667-5809 Email Electronic Databases Open Access Theses and Dissertations - Results limited to freely accessible, full-text ETDs Center for Research Libraries - Foreign Dissertations Collection Theses Canada - Canadian theses from Library and Archives Canada EThOS (Electronic Theses Online Service) - UK theses from the British Library Networked Digital Library of Theses and

  2. Find eBooks

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    eBooks Find eBooks Many of these resources are Lab-only and will require Remote Access. Question? 667-5809 Email The Library Catalog offers the most comprehensive search for scientific Ebooks. The following is a selection of Ebook sources, in case you want to search them directly. [See also Conference Proceedings.] Key eBook Providers Ebook Central information - online access to thousands of ebooks across scientific subject areas Elsevier ScienceDirect - Includes books, book series, and

  3. Transcription factor-based biosensor

    DOEpatents

    2013-10-08

    The present invention provides for a system comprising a BmoR transcription factor, a .sigma..sup.54-RNA polymerase, and a pBMO promoter operatively linked to a reporter gene, wherein the pBMO promoter is capable of expression of the reporter gene with an activated form of the BmoR and the .sigma..sup.54-RNA polymerase.

  4. Engaging Financial Institution Partners Transcript.doc | Department...

    Office of Environmental Management (EM)

    Engaging Financial Institution Partners Transcript.doc Engaging Financial Institution Partners Transcript.doc Engaging Financial Institution Partners Transcript.doc Microsoft ...

  5. Electricity Advisory Committee Meeting, May 20, 2008 (TRANSCRIPT...

    Energy Saver

    (TRANSCRIPT) Electricity Advisory Committee Meeting, May 20, 2008 (TRANSCRIPT) Transcript of the May 20, 2008 Electricity Advisory Committee Meeting including a FERC presentation ...

  6. Financing Residential Energy Efficiency with Carbon Offsets Transcript...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Residential Energy Efficiency with Carbon Offsets Transcript Financing Residential Energy Efficiency with Carbon Offsets Transcript This document contains the transcript for the ...

  7. Transcript from 12/01/08 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    1/08 Transcript from 12/01/08 Transcript from 12/01/08 (14.79 MB) More Documents & Publications Transcript from 12/05/08 Manufacturing Pre-Solicitation Transcript Speaker Bios: Matthew McMillen

  8. Finding of No Significant Impact

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    U.S. Department of Energy Finding of No Significant Impact 2 June 2001 This page intentionally left blank. U.S. Department of Energy Finding of No Significant Impact 12 June 2001 This page intentionally left blank.

  9. Using regulations.gov to find dockets and documents

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    regulations.gov to find dockets and documents Regulations.gov is a web-based repository of Federal regulatory documents. Publicly accessible documents associated with DOE Appliance and Equipment Standards rulemakings and related Federal Register notices are now accessible on regulations.gov On regulations.gov, you can: View Proposed and Final Rules and other regulatory documents or notices Read public comments, as well as public meeting presentations and transcripts These documents are part of a

  10. Finding of No Significant Impact

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Bonneville Power Administration Northern Mid-Columbia Joint Project Finding of No Significant Impact March 2016 Bonneville Power Administration 1 Northern Mid-Columbia Joint Project DEPARTMENT OF ENERGY Bonneville Power Administration Finding of No Significant Impact (FONSI) and Floodplain Statement of Findings DOE/EA-1945 Summary: Bonneville Power Administration (BPA) announces its environmental findings on one of the routing alternatives - West Route D-E - identified for the Northern

  11. ARM - Research Highlights: Notable Findings

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    HighlightsNotable Research Findings Form Submit a New Research Highlight Sort Highlights Submitter Title Research Area Working Group Submission Date DOE Progress Reports Notable Research Findings for 2001-2006 Office of Science Abstracts Database Research Highlights Summaries Notable Research Findings for the Past Five Years Aerosols Cloud Parameterization and Modeling (Currently Cloud Modeling) Cloud Properties Instantaneous Radiative Flux (Currently Radiative Processes)

  12. Manufacturing Pre-Solicitation Transcript | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Pre-Solicitation Transcript Manufacturing Pre-Solicitation Transcript Transcript from the US DOE Hydrogen Program Manufacturing R&D Pre-Solicitation Meeting pre_solicitation_transcript.pdf (419.1 KB) More Documents & Publications Transcript from 12/05/08 Transcript from 12/01/08 Public Roundtable- Data Access and Privacy Issues Related to Smart Grid Technologies

  13. SERC Photovoltaics for Residential Buildings Webinar Transcript |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy Photovoltaics for Residential Buildings Webinar Transcript SERC Photovoltaics for Residential Buildings Webinar Transcript A presentation sponsored by the U.S. Department of Energy about using solar photovoltaics (PV) systems to provide electricity for homes. 20110125_pv_webinar.pdf (109.9 KB) More Documents & Publications Sustainable Energy Resources for Consumers (SERC) - Solar Hot Water Solar Webinar Text Version TAP Webcast Transcript July-29, 2009

  14. Isolated Fungal Promoters and Gene Transcription Terminators...

    Office of Scientific and Technical Information (OSTI)

    The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first ...

  15. Find R&D Results

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Find technical reports, journal articles, accepted manuscripts, conference papers, patents, theses, books, multimedia, and data information. Use the basic or advanced search to get ...

  16. Find a Job | Careers | NREL

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Find a Job We're looking for scientists, research technicians, engineers, analysts, managers and project leaders, administrators, and communicators, along with information technology, security, facilities, and clerical/administrative support specialists. Interested in joining our team? Find your opportunity We're passionate about our mission-to develop clean energy and energy efficiency technologies and practices, advance related science and engineering, and provide knowledge and innovations to

  17. csep_transcript_transportation.doc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    transportation.doc csep_transcript_transportation.doc csep_transcript_transportation.doc csep_transcript_transportation.doc (48 KB) More Documents & Publications csep_transcript_minneapolis.doc Marketing & Driving Demand: Social Media Tools & Strategies - January 16, 2011 (Text Version) Transcript: Biomass Clean Cities Webinar - Workforce Development

  18. Transcript from 12/05/08 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    5/08 Transcript from 12/05/08 Transcript from 12/05/08 (176.91 KB) More Documents & Publications Transcript from 12/01/08 Manufacturing Pre-Solicitation Transcript SERC Photovoltaics for Residential Buildings Webinar Transcript

  19. transcript_pv_mapper.doc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    transcript_pv_mapper.doc transcript_pv_mapper.doc transcript_pv_mapper.doc transcript_pv_mapper.doc (74.5 KB) More Documents & Publications PVMapper: A Tool for Energy Siting transcript_jedi_model.doc 2009 National Electric Transmission Congestion Study - San Francisco Workshop

  20. Telomere-surrounding regions are transcription-permissive 3D nuclear compartments in human cells

    SciTech Connect

    Quina, Ana Sofia; Parreira, Leonor . E-mail: lparreir@igc.gulbenkian.pt

    2005-07-01

    Positioning of genes relative to nuclear heterochromatic compartments is thought to help regulate their transcriptional activity. Given that human subtelomeric regions are rich in highly expressed genes, we asked whether human telomeres are related to transcription-permissive nuclear compartments. To address this question, we investigated in the nuclei of normal human lymphocytes the spatial relations of two constitutively expressed genes (ACTB and RARA) and three nuclear transcripts (ACTB, IL2RA and TCRB) to telomeres and centromeres, as a function of gene activity and transcription levels. We observed that genes and gene transcripts locate close to telomere clusters and away from chromocenters upon activation of transcription. These findings, together with the observation that SC35 domains, which are enriched in pre-mRNA processing factors, are in close proximity to telomeres, indicate that telomere-neighboring regions are permissive to gene expression in human cells. Therefore, the associations of telomeres observed in the interphase nucleus might contribute, as opposed to chromocenters, for the establishment of transcription-permissive 3D nuclear compartments.

  1. Electricity Advisory Committee Meeting, September 25, 2008: Transcript...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Transcript Electricity Advisory Committee Meeting, September 25, 2008: Transcript Transcript of the Electricity Advisory Committee Meeting 3:30 p.m. through 5:55 p.m on Thursday, ...

  2. Peak finding using biorthogonal wavelets

    SciTech Connect

    Tan, C.Y.

    2000-02-01

    The authors show in this paper how they can find the peaks in the input data if the underlying signal is a sum of Lorentzians. In order to project the data into a space of Lorentzian like functions, they show explicitly the construction of scaling functions which look like Lorentzians. From this construction, they can calculate the biorthogonal filter coefficients for both the analysis and synthesis functions. They then compare their biorthogonal wavelets to the FBI (Federal Bureau of Investigations) wavelets when used for peak finding in noisy data. They will show that in this instance, their filters perform much better than the FBI wavelets.

  3. TAP Webcast Transcript July-29, 2009

    Energy.gov [DOE]

    Transcript of the audio for the July 2009 TAP Webcast titled Economic Impacts of Renewable Energy sponsored by the U.S. Department of Energy (DOE) Technical Assistance Project (TAP) for state and local officials.

  4. Writing audit findings: Be reasonable!

    SciTech Connect

    Girvin, N.W.

    1992-05-01

    A customary approach to auditing and reporting deficiencies is to keep a running list of those that are found, evaluate the severity of each, and based on the evidence, document findings or observations or concerns in an audit report. The report is issued and the auditee is normally requested to address ``root cause`` as part of their corrective action. This paper describes a ``root problems`` approach to documenting audit findings that is designed not only to put the QA auditor in a more favorable light, but to more effectively enable the auditee to identify root cause and meaningful corrective action. The positive results of this approach are considerable. You will have fewer findings but those you do have will be substantial. You will cite requirements that sound reasonable and make arguments difficult. If some of the supporting deficiencies (examples) prove to be incorrect, you will still have ample support for the original finding. You will be seen as reasonable individual who can help lead the auditee towards identification of root cause without taking away part of the responsibility. You even have a fair chance of fostering a sense of commitment to quality improvement on the auditee`s part. This in itself, is its own reward.

  5. Writing audit findings: Be reasonable

    SciTech Connect

    Girvin, N.W.

    1992-05-01

    A customary approach to auditing and reporting deficiencies is to keep a running list of those that are found, evaluate the severity of each, and based on the evidence, document findings or observations or concerns in an audit report. The report is issued and the auditee is normally requested to address root cause'' as part of their corrective action. This paper describes a root problems'' approach to documenting audit findings that is designed not only to put the QA auditor in a more favorable light, but to more effectively enable the auditee to identify root cause and meaningful corrective action. The positive results of this approach are considerable. You will have fewer findings but those you do have will be substantial. You will cite requirements that sound reasonable and make arguments difficult. If some of the supporting deficiencies (examples) prove to be incorrect, you will still have ample support for the original finding. You will be seen as reasonable individual who can help lead the auditee towards identification of root cause without taking away part of the responsibility. You even have a fair chance of fostering a sense of commitment to quality improvement on the auditee's part. This in itself, is its own reward.

  6. Find Internships | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Internships Find Internships Internships in energy efficiency and renewable energy-related fields, such as solar and fuel cells, energy efficiency, atmospheric sciences, ecology, global carbon cycles, climatology, and more, include EERE's own student internship program. Featured Internships Clean Cities University Workforce Development Program Clean Cities logo Science Undergraduate Laboratory Internships (SULI) Fall 2013 Picture of tow people in a labratory working Minority Educational

  7. Engineering an allosteric transcription factor to respond to...

    Office of Scientific and Technical Information (OSTI)

    Engineering an allosteric transcription factor to respond to new ligands Citation Details In-Document Search Title: Engineering an allosteric transcription factor to respond to new ...

  8. Electricity Advisory Committee Meeting, October 29, 2010: Transcript...

    Energy.gov [DOE] (indexed site)

    Transcript of the Electricity Advisory Committee Meeting held on October 29, 2010. Electricity Advisory Committee Meeting, October 29, 2010: Transcript (938.59 KB) More Documents & ...

  9. Webinar-Transcript-Repeat.pdf | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Webinar-Transcript-Repeat.pdf Webinar-Transcript-Repeat.pdf (94.89 KB) More Documents & Publications Title Title Slide 1

  10. FINDING OF NO SIGNIFICANT IMPACT

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    COMMERCIAL DEMONSTRATION OF THE MANUFACTURED AGGREGATE PROCESSING TECHNOLOGY UTILIZING SPRAY DRYER ASH 1 AGENCY: U.S. Department of Energy (DOE) ACTION: Finding of No Significant Impact (FONSI) SUMMARY: The DOE has prepared an Environmental Assessment (EA), DOE/EA-1449, to analyze the potential environmental consequences of participating in a cooperative agreement with Universal Aggregates, LLC, for the design, construction, and operation of a plant to manufacture lightweight aggregates. The

  11. Find Jobs | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Jobs Find Jobs Clean energy jobs can be found in the public, private, and nonprofit sectors and can range from entry-level to professional positions in a wide range of fields. This section can help you familiarize yourself with different types of green energy jobs as you begin your search for a job that meets your interests and skills. EERE Career Opportunities EERE Career Opportunities Energy Efficency and Renewable Energy, the DOE office leading the nation's effort to enhance energy efficiency

  12. CSC Workshop Transcript 02-20-2105 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Workshop Transcript 02-20-2105 CSC Workshop Transcript 02-20-2105 CSC Workshop Transcript 02-20-2105 (687.86 KB) More Documents & Publications Transcript of the Public Workshop EA-1971: FERC Notice of Intent to Prepare an Environmental Assessment EIS-0501: Notice of Intent

  13. csep_transcript_minneapolis.doc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    minneapolis.doc csep_transcript_minneapolis.doc csep_transcript_minneapolis.doc csep_transcript_minneapolis.doc (60.5 KB) More Documents & Publications newguideforcommunityenergystrategicplanning.doc csep_transcript_aspen.doc Marketing & Driving Demand: Social Media Tools & Strategies - January 16, 2011 (Text Version)

  14. Department of Energy (DOE) Webinar Transcript | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    (DOE) Webinar Transcript Department of Energy (DOE) Webinar Transcript Department of Energy (DOE) Webinar Transcript (95.26 KB) More Documents & Publications Welcome to the Department of Energy&#8217;s Recovery Act webinar on reporting in to federalreporting Department of Energy (DOE) Webinar Transcript Title

  15. Transcript of the Public Workshop | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Transcript of the Public Workshop Transcript of the Public Workshop Transcript of the Public Workshop (237.04 KB) More Documents & Publications CSC Workshop Transcript 02-20-2105 3116 Public Meeting Summaries - November 2006 Nuclear Energy Institute (NEI) Ex Parte

  16. Transcription factor-based biosensors for detecting dicarboxylic acids

    DOEpatents

    Dietrich, Jeffrey; Keasling, Jay

    2014-02-18

    The invention provides methods and compositions for detecting dicarboxylic acids using a transcription factor biosensor.

  17. Transcriptional enhancer from milk protein genes

    DOEpatents

    Casperson, Gerald F.; Schmidhauser, Christian T.; Bissell, Mina J.

    1999-01-01

    The invention relates to novel enhancer nucleotide sequences which stimulate transcription of heterologous DNA in cells in culture. The enhancers are derived from major milk protein genes by the process of deletion mapping and functional analysis. The invention also relates to expression vectors containing the novel enhancers.

  18. Regional Transmission Projects: Finding Solutions

    SciTech Connect

    The Keystone Center

    2005-06-15

    The Keystone Center convened and facilitated a year-long Dialogue on "Regional Transmission Projects: Finding Solutions" to develop recommendations that will help address the difficult and contentious issues related to expansions of regional electric transmission systems that are needed for reliable and economic transmission of power within and across regions. This effort brought together a cross-section of affected stakeholders and thought leaders to address the problem with the collective wisdom of their experience and interests. Transmission owners sat at the table with consumer advocates and environmental organizations. Representatives from regional transmission organizations exchanged ideas with state and federal regulators. Generation developers explored common interests with public power suppliers. Together, the Dialogue participants developed consensus solutions about how to begin unraveling some of the more intractable issues surrounding identification of need, allocation of costs, and reaching consensus on siting issues that can frustrate the development of regional transmission infrastructure. The recommendations fall into three broad categories: 1. Recommendations on appropriate institutional arrangements and processes for achieving regional consensus on the need for new or expanded transmission infrastructure 2. Recommendations on the process for siting of transmission lines 3. Recommendations on the tools needed to support regional planning, cost allocation, and siting efforts. List of Dialogue participants: List of Dialogue Participants: American Electric Power American Transmission Company American Wind Energy Association California ISO Calpine Corporation Cinergy Edison Electric Institute Environmental Defense Federal Energy Regulatory Commission Great River Energy International Transmission Company ISO-New England Iowa Public Utility Board Kanner & Associates Midwest ISO National Association of Regulatory Utility Commissioners National Association

  19. Method to determine transcriptional regulation pathways in organisms

    DOEpatents

    Gardner, Timothy S.; Collins, James J.; Hayete, Boris; Faith, Jeremiah

    2012-11-06

    The invention relates to computer-implemented methods and systems for identifying regulatory relationships between expressed regulating polypeptides and targets of the regulatory activities of such regulating polypeptides. More specifically, the invention provides a new method for identifying regulatory dependencies between biochemical species in a cell. In particular embodiments, provided are computer-implemented methods for identifying a regulatory interaction between a transcription factor and a gene target of the transcription factor, or between a transcription factor and a set of gene targets of the transcription factor. Further provided are genome-scale methods for predicting regulatory interactions between a set of transcription factors and a corresponding set of transcriptional target substrates thereof.

  20. Mars Rover finds changing rocks, surprising scientists

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Mars Rover finds changing rocks, surprising scientists Mars Rover finds changing rocks, surprising scientists As NASA's Curiosity rover treks up a three-mile-high mountain on Mars, ...

  1. Fernald Environmental Management Project Director's Final Findings...

    Office of Environmental Management (EM)

    Environmental Protection Agency ("Ohio EPA") in Ohio Revised Code ("ORC") ... I-V. FINDINGS OF FACT The Director of the Ohio EPA has determined the following findings ...

  2. Finding Hidden Oil and Gas Reserves

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Finding Hidden Oil and Gas Reserves Finding Hidden Oil and Gas Reserves Key Challenges: Seismic imaging methods, vital in our continuing search for deep offshore oil and gas...

  3. HIV transcription is induced in dying cells

    SciTech Connect

    Woloschak, G.E.; Chang-Liu, Chin-Mei; Schreck, S. |; Panozzo, J.; Libertin, C.R.

    1996-02-01

    Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. {gamma} rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that {gamma}-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. Doses which caused over 99% cell killing induced HIV-LTR transcription maximally, demonstrating that cells that will go on to die by 14 days are the cells expressing HIV-LTR-CAT.

  4. Department of Energy (DOE) Webinar Transcript | Department of Energy

    Energy.gov [DOE] (indexed site)

    Department of Energy (DOE) Webinar Transcript (145.56 KB) More Documents & Publications Welcome to the Department of Energy&#8217;s Recovery Act webinar on reporting in to federalreporting Department of Energy (DOE) Webinar Transcript Title

  5. Financing Residential Energy Efficiency with Carbon Offsets Transcript

    Energy.gov [DOE]

    This document contains the transcript for the Financing Residential Energy Efficiency with Carbon Offsets webinar held on March 12, 2013.

  6. stat_webinar_070913_transcript.doc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    stat_webinar_070913_transcript.doc stat_webinar_070913_transcript.doc stat_webinar_070913_transcript.doc stat_webinar_070913_transcript.doc (64.5 KB) More Documents & Publications Final Report -Regulatory and Utility Solutions to Advance SunShot Initiative Goals Connecting-to-the-Grid-Guide-6th-edition.pdf Community Renewables: Model Program Rules

  7. Public Meeting Transcript: Sustainable Design Standards for Federal Buildings

    Energy.gov [DOE]

    This transcript is from the public meeting to discuss sustainable design standards for Federal buildings held on July 28, 2010.

  8. 20130226_sunshot_transcript_lbnl.doc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    transcript_lbnl.doc 20130226_sunshot_transcript_lbnl.doc 20130226_sunshot_transcript_lbnl.doc 20130226_sunshot_transcript_lbnl.doc (73.5 KB) More Documents & Publications Why Are Resiential PV Prices in Germany So Much Lower Than in the United States? text_alternative_stat_webinar_2012_7_25.docx An Analysis of Residential PV System Price Differences between the United States and Germany

  9. Blackout 2003: Transcript of Technical Conference with the Electric System

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Investigation Team | Department of Energy Transcript of Technical Conference with the Electric System Investigation Team Blackout 2003: Transcript of Technical Conference with the Electric System Investigation Team Electric System Investigation Team: 8 Reliability Recommendation consultation Transcript of the technical conference sponsored by the U.S. Canada task force investigating the August 14, 2003 blackout across Canada and the Northeastern United States. The transcript includes reports

  10. CSC Transcript of Information Session | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Transcript of Information Session CSC Transcript of Information Session CSC Transcript of Information Session (2.04 MB) More Documents & Publications CSC Notice of Public Meeting Convention on Supplementary Compensation Rulemaking Enforcement Notice of Intent to Investigate, Computer Sciences Corporation

  11. csep_transcript_aspen.doc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    aspen.doc csep_transcript_aspen.doc csep_transcript_aspen.doc csep_transcript_aspen.doc (45.5 KB) More Documents & Publications highperformanceleasingstrategiesforstateandlocalgovernments.doc Using Social Media to Engage the Community in Energy Efficiency Projects.doc webinar_innovation_permitting_inspections

  12. Canfield's Couplet Clues for Finding Superconductors

    SciTech Connect

    Canfield, Paul

    2015-04-28

    Ames Laboratory scientist, Paul Canfield, summarizes his thoughts about finding new superconducting materials.

  13. Canfield's Couplet Clues for Finding Superconductors

    ScienceCinema

    Canfield, Paul

    2015-06-30

    Ames Laboratory scientist, Paul Canfield, summarizes his thoughts about finding new superconducting materials.

  14. TE Connectivity Finds Answers in Tomography

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    TE Connectivity Finds Answers in Tomography TE Connectivity Finds Answers in Tomography Print Thursday, 22 August 2013 10:50 TE Connectivity is a world leader in connectivity-the...

  15. National Electric Transmission Congestion Study: Preliminary Findings |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy Study: Preliminary Findings National Electric Transmission Congestion Study: Preliminary Findings The Department hosted three webinars in August 2012 to receive input and suggestions concerning the preliminary findings of the National Electric Transmission Congestion Study. The updated presentation used in the webinars is now available. National Electric Transmission Congestion Study: Preliminary Findings - updated presentation (908.32 KB) More Documents &

  16. Fossil Energy Word Find | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Word Find Fossil Energy Word Find Word Find (inlcuidng answer key) Fossil Energy Word Search (96.6 KB) More Documents & Publications Fossil Energy Crossword Puzzle Coal Study Guide for Elementary School Guide to Low-Emission Boiler and Combustion Equipment Selection

  17. Fall Lectures Feature Life of Einstein; Exploring Our World With Particle

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    5 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files are the unedited transcripts of EIA's fall 2005 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts, here at:

  18. Fall 2005 Meeting of the ASA

    Energy Information Administration (EIA) (indexed site)

    5 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files are the unedited transcripts of EIA's fall 2005 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts, here at:

  19. June 2014 Electricity Advisory Committee Meeting Summaries and Transcripts

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Now Available | Department of Energy 2014 Electricity Advisory Committee Meeting Summaries and Transcripts Now Available June 2014 Electricity Advisory Committee Meeting Summaries and Transcripts Now Available August 13, 2014 - 11:17am Addthis The meeting summaries and transcripts for the Electricity Advisory Committee (EAC) meeting held on June 16-17, 2014, are now available. The EAC provides advice to the U.S. Department of Energy in implementing the Energy Policy Act of 2005, executing

  20. Reducing nontemplated 3' nucleotide addition to polynucleotide transcripts

    DOEpatents

    Kao, C. Cheng

    2000-01-01

    Non-template 3' nucleotide addition to a transcript is reduced by transcribing a transcript from a template comprising an ultimate and/or penultimate 5' ribose having a C'2 substituent such as methoxy, which reduces non-template 3' nucleotide addition to the transcript. The methods are shown to be applicable to a wide variety of polymerases, including Taq, T7 RNA polymerase, etc.

  1. Unlocking the Mysteries of the J. Robert Oppenheimer Transcript |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy the Mysteries of the J. Robert Oppenheimer Transcript Unlocking the Mysteries of the J. Robert Oppenheimer Transcript October 3, 2014 - 2:48pm Addthis This is an excerpt from the original press release from the Atomic Energy Commission announcing the publication of the redacted Oppenheimer transcript. | Photo courtesy of the Energy Department. This is an excerpt from the original press release from the Atomic Energy Commission announcing the publication of the redacted

  2. HIV transcription is induced in dying cells

    SciTech Connect

    Woloschak, G.E.; Chang-Liu, Chin-Mei; Schreck, S. |

    1995-06-01

    Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. {gamma} rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that {gamma}-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. 14 refs., 4 figs., 1 tab.

  3. transcript_jedi_model.doc | Department of Energy

    Energy.gov [DOE] (indexed site)

    document icon transcriptjedimodel.doc More Documents & Publications TAP Webcast Transcript July-29, 2009 Scenario Jedi Tools for Designing & Implementing Better Finance Programs

  4. Transcript: OSTI Highlights First-Time Electronic Availability...

    Office of Scientific and Technical Information (OSTI)

    Transcript: OSTI Highlights First-Time Electronic Availability of Geothermal Documents at San Diego Geothermal Conference, Office of Scientific and Technical Information, U.S. ...

  5. Transcript of March 28, 2013, TAP webinar titled Internal Benchmarking...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    TAP webinar titled Internal Benchmarking Outreach and Data Collection Techniques Transcript of March 28, 2013, TAP webinar titled Internal Benchmarking Outreach and Data ...

  6. Partnering With Utilities to Offer Energy Efficiency Programs Webinar Transcript

    Energy.gov [DOE]

    Partnering With Utilities to Offer Energy Efficiency Programs Webinar Transcript, from the U.S. Department of Energy Technical Assistance Program (TAP).

  7. Blackout 2003: Transcript of Technical Conference with the Electric...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Transcript of the technical conference sponsored by the U.S. Canada task force investigating the August 14, 2003 blackout across Canada and the Northeastern United States. The ...

  8. Roger D. Kornberg Polymerase, DNA, RNA, and Transcription

    Office of Scientific and Technical Information (OSTI)

    Kornberg's studies have provided an understanding at the atomic level of how the process of transcription occurs and also how it is controlled. Because the regulation of ...

  9. Right rock: Finding/refining customer expectations

    SciTech Connect

    Ashby, R.

    1997-11-01

    This report contains viewgraphs on methods of finding customer expectations of software and then refining the documentation and software to fit these expectations.

  10. Distributed PV Interconnection: Recent Analysis Findings

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    survey of utility interconnection practices. o SEPA finds that utilities confront common challenges as they move towards more streamlined interconnection application ...

  11. Distributed PV Interconnection Recent Analysis Findings

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Thank you for joining the Distributed Generation Interconnection Collaborative ... which each will discuss recent research and analysis findings related to interconnection. ...

  12. ORISE: Forensic scientist finds truth in fingerprints

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Forensic scientist finds truth in fingerprints For more than 30 years, Imogene Van Buren has searched for truth in the friction ridges of fingerprints. She has identified victims...

  13. Conservation of Transcription Start Sites within Genes across a Bacterial Genus

    SciTech Connect

    Shao, Wenjun; Price, Morgan N.; Deutschbauer, Adam M.; Romine, Margaret F.; Arkin, Adam P.

    2014-07-01

    Transcription start sites (TSSs) lying inside annotated genes, on the same or opposite strand, have been observed in diverse bacteria, but the function of these unexpected transcripts is unclear. Here, we use the metal-reducing bacterium Shewanella oneidensis MR-1 and its relatives to study the evolutionary conservation of unexpected TSSs. Using high-resolution tiling microarrays and 5'-end RNA sequencing, we identified 2,531 TSSs in S. oneidensis MR-1, of which 18% were located inside coding sequences (CDSs). Comparative transcriptome analysis with seven additional Shewanella species revealed that the majority (76%) of the TSSs within the upstream regions of annotated genes (gTSSs) were conserved. Thirty percent of the TSSs that were inside genes and on the sense strand (iTSSs) were also conserved. Sequence analysis around these iTSSs showed conserved promoter motifs, suggesting that many iTSS are under purifying selection. Furthermore, conserved iTSSs are enriched for regulatory motifs, suggesting that they are regulated, and they tend to eliminate polar effects, which confirms that they are functional. In contrast, the transcription of antisense TSSs located inside CDSs (aTSSs) was significantly less likely to be conserved (22%). However, aTSSs whose transcription was conserved often have conserved promoter motifs and drive the expression of nearby genes. Overall, our findings demonstrate that some internal TSSs are conserved and drive protein expression despite their unusual locations, but the majority are not conserved and may reflect noisy initiation of transcription rather than a biological function.

  14. Transcription factor FOXA2-centered transcriptional regulation network in non-small cell lung cancer

    SciTech Connect

    Jang, Sang-Min; An, Joo-Hee; Kim, Chul-Hong; Kim, Jung-Woong Choi, Kyung-Hee

    2015-08-07

    Lung cancer is the leading cause of cancer-mediated death. Although various therapeutic approaches are used for lung cancer treatment, these mainly target the tumor suppressor p53 transcription factor, which is involved in apoptosis and cell cycle arrest. However, p53-targeted therapies have limited application in lung cancer, since p53 is found to be mutated in more than half of lung cancers. In this study, we propose tumor suppressor FOXA2 as an alternative target protein for therapies against lung cancer and reveal a possible FOXA2-centered transcriptional regulation network by identifying new target genes and binding partners of FOXA2 by using various screening techniques. The genes encoding Glu/Asp-rich carboxy-terminal domain 2 (CITED2), nuclear receptor subfamily 0, group B, member 2 (NR0B2), cell adhesion molecule 1 (CADM1) and BCL2-associated X protein (BAX) were identified as putative target genes of FOXA2. Additionally, the proteins including highly similar to heat shock protein HSP 90-beta (HSP90A), heat shock 70 kDa protein 1A variant (HSPA1A), histone deacetylase 1 (HDAC1) and HDAC3 were identified as novel interacting partners of FOXA2. Moreover, we showed that FOXA2-dependent promoter activation of BAX and p21 genes is significantly reduced via physical interactions between the identified binding partners and FOXA2. These results provide opportunities to understand the FOXA2-centered transcriptional regulation network and novel therapeutic targets to modulate this network in p53-deficient lung cancer. - Highlights: • Identification of new target genes of FOXA2. • Identifications of novel interaction proteins of FOXA2. • Construction of FOXA2-centered transcriptional regulatory network in non-small cell lung cancer.

  15. Missourian Finds New Opportunity in Energy Industry

    Energy.gov [DOE]

    How one Missouri man decided to take matters into his own hands - creating a company that improves energy efficiency in homes and buildings - after not being able to find work during the recession.

  16. Distributed PV Interconnection Recent Analysis Findings

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Recent Analysis Findings Page 1 of 18 Kristen Ardani, Miriam Makhyoun Page 1 of 18 [Speaker: Kristen Ardani] Cover Slide: Good afternoon, everyone. Thank you for joining the Distributed Generation Interconnection Collaborative informational webinar. Today, we are kicking off 2015 with a joint presentation from SEPA and NREL, in which each will discuss recent research and analysis findings related to interconnection. Slide 2: So really, the purpose of today's meeting is to hear recent research

  17. Distributed PV Interconnection: Recent Analysis Findings

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    January 21, 2015 "NREL and SEPA Recent Analysis Findings" Miriam Makhyoun, Solar Electric Power Association (SEPA) Kristen Ardani, National Renewable Energy Laboratory (NREL) 2 Purpose of Today's Meeting * Hear results from SEPA's recent survey of utility interconnection practices. o SEPA finds that utilities confront common challenges as they move towards more streamlined interconnection application processing. * Hear NREL results of forthcoming DGIC data collection and analysis,

  18. Mars Rover finds changing rocks, surprising scientists

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Mars Rover finds changing rocks, surprising scientists Mars Rover finds changing rocks, surprising scientists As NASA's Curiosity rover treks up a three-mile-high mountain on Mars, the rocks are changing. Back on Earth, scientists analyzing the data realized this was something different: It turned out to be the first of the high-silica rocks. December 24, 2015 Mars landscape This color-adjusted composite of images taken by NASA's Curiosity rover in September shows the lower portion of Mount

  19. Floodplain Assessment and Statement of Findings

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    and Statement of Findings for Installation of a Renewable Energy Anaerobic Digester Facility at the University of California, Davis in Yolo County, California January 28, 2013 1.0 Introduction This Floodplain Assessment and Statement of Findings has been prepared in accordance with 10 Code of Federal Regulations (CFR) 1022, "Compliance with Floodplain and Wetland Environmental Review Requirements" which were promulgated to implement the requirements of the U.S. Department of Energy's

  20. Floodplain Statement of Findings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Floodplain Statement of Findings Floodplain Statement of Findings Loan Guarantee to AES Energy Storage, LLC Project Dyno Electric Grid Stability Advanced Battery Systems Project Dyno would provide ancillary services within the New York power market. The Project is composed of advanced lithium ion battery cells and power control technologies to store power to help maintain the stability of the electric power grid. The Project would be composed of ten fifty-three foot metal containers each housing

  1. Jefferson Laboratory Findings Excite Theoreticians, Experimentatlists |

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Jefferson Lab Laboratory Findings Excite Theoreticians, Experimentatlists Jefferson Laboratory Findings Excite Theoreticians, Experimentatlists March 1999 Seemingly reasonable assumptions define the human view of the world. But ruled of thumb can mislead - or be altogether incorrect. Fortunately, in physics, most assumptions can be tested. Those that don't measure up are amended. An experiment at the Department of Energy's Thomas Jefferson National Accelerator Facility (Jefferson Lab) has

  2. Microsoft Word - Consolidated Verbatim Transcript CBS Meeting Sacramento California Transcripts_Final

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Holiday Inn Capitol Plaza Sacramento 300 J Street Sacramento, CA 95814 April 26, 2016 VERBATIM TRANSCRIPT Mr. Jim Hamilton. Good afternoon. And for those in later time zones via webinar, good evening. Welcome, and thank you for being here today. My name is Jim Hamilton. I'm an advisor to the Department of Energy's Consent-Based Siting Team and my role here today is to help us all to have a good, open, productive conversation. To start off we have a few housekeeping issues I want to talk about

  3. Transcriptional bursting explains the noise–versus–mean relationship in mRNA and protein levels

    DOE PAGES [OSTI]

    Dar, Roy; Shaffer, Sydney M.; Singh, Abhyudai; Razooky, Brandon S.; Simpson, Michael L.; Raj, Arjun; Weinberger, Leor S.

    2016-07-28

    Recent analysis demonstrates that the HIV-1 Long Terminal Repeat (HIV LTR) promoter exhibits a range of possible transcriptional burst sizes and frequencies for any mean-expression level. However, these results have also been interpreted as demonstrating that cell-tocell expression variability (noise) and mean are uncorrelated, a significant deviation from previous results. Here, we re-examine the available mRNA and protein abundance data for the HIV LTR and find that noise in mRNA and protein expression scales inversely with the mean along analytically predicted transcriptional burst-size manifolds. We then experimentally perturb transcriptional activity to test a prediction of the multiple burst-size model: thatmore » increasing burst frequency will cause mRNA noise to decrease along given burst-size lines as mRNA levels increase. In conclusion, the data show that mRNA and protein noise decrease as mean expression increases, supporting the canonical inverse correlation between noise and mean.« less

  4. Lab employee helps veterans find work

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Lab employee helps veterans find work Community Connections: Your link to news and opportunities from Los Alamos National Laboratory Latest Issue:November 2, 2016 all issues All Issues » submit Lab employee helps veterans find work Cody Nevala-Amraen recruits vets to Los Alamos. November 1, 2016 Ken and Laura McClellan traveled to the Ha-Mokuba village in Lesotho, Africa, to help build a new home for the Lecheko family. Cody Nevala-Amraen is a U.S. Marine Corps veteran who served in Iraq and

  5. An interesting finding in sup 229 Th

    SciTech Connect

    Reich, C.W.

    1990-01-01

    Work at INEL has recently established that the first excited states of {sup 229}Th forms a closely spaced doublet with the ground state, the separation being 1 {plus minus} 4 electron volts. A discussion of the data and the reasoning supporting this unprecedented finding is given. Some potential applications are briefly mentioned. 27 refs., 3 figs., 2 tabs.

  6. Finding a Career in Energy Efficiency

    Energy.gov [DOE]

    Kendra went to a county agency hoping they could help her find work, but what she really wanted was a career. That’s when she was referred to an upcoming weatherization training program hosted by the Laborers’ International Union of North America, a half-million strong labor union for construction workers, including weatherization workers.

  7. Transcript of First Steps Toward Developing Renewable Energy...

    Energy Saver

    Transcript of First Steps Toward Developing Renewable Energy and Energy Efficiency on Tribal ... The cost share must come from non-Federal sources unless otherwise allowed by law. I ...

  8. Transcript, Keeping the Edge: Enhancing Performance by Managing Culture

    Energy.gov [DOE]

    Transcript: 2003 INPO CEO Conference, Keeping the Edge: “Enhancing Performance by Managing Culture” Edgar H. Schein, Ph.D. Professor Emeritis, Massachusetts Institute of Technology November 7, 2003

  9. Public Meeting Transcript: Sustainable Design Standards for Federal...

    Energy.gov [DOE] (indexed site)

    This transcript is from the public meeting to discuss sustainable design standards for Federal buildings held on July 28, 2010. noprmeeting072810.pdf (345.56 KB) More Documents & ...

  10. Roger D. Kornberg Polymerase, DNA, RNA, and Transcription

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Roger D. Kornberg Polymerase, DNA, RNA, and Transcription Resources with Additional Information Roger D. Kornberg Credit: Linda A. Cicero/ Stanford News Service Roger D. Kornberg was awarded the 2006 Nobel Prize in Chemistry "for his studies of the molecular basis of eukaryotic transcription". He determined 'how DNA's genetic blueprint is read and used to direct the process for protein manufacture. Kornberg carried out a significant part of the research leading to this prize at the

  11. Lab supercomputer finds new home at UNM

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Lab supercomputer to UNM Community Connections: Your link to news and opportunities from Los Alamos National Laboratory Latest Issue:November 2, 2016 all issues All Issues » submit Lab supercomputer finds new home at UNM Chance of a lifetime for students with complex research projects March 1, 2015 UNM logo. UNM logo. Contact Community Programs Director Kurt Steinhaus Email Editor Ute Haker Email The New Mexico Consortium has donated Los Alamos National Laboratory's Ulam supercomputer to the

  12. Best Possible Strategy for Finding Ground States

    SciTech Connect

    Franz, Astrid; Hoffmann, Karl Heinz; Salamon, Peter

    2001-06-04

    Finding the ground state of a system with a complex energy landscape is important for many physical problems including protein folding, spin glasses, chemical clusters, and neural networks. Such problems are usually solved by heuristic search methods whose efficacy is judged by empirical performance on selected examples. We present a proof that, within the large class of algorithms that simulate a random walk on the landscape, threshold accepting is the best possible strategy. In particular, it can perform better than simulated annealing and Tsallis statistics. Our proof is the first example of a provably optimal strategy in this area.

  13. Transcription Factors Bind Thousands of Active and InactiveRegions in the Drosophila Blastoderm

    SciTech Connect

    Li, Xiao-Yong; MacArthur, Stewart; Bourgon, Richard; Nix, David; Pollard, Daniel A.; Iyer, Venky N.; Hechmer, Aaron; Simirenko, Lisa; Stapleton, Mark; Luengo Hendriks, Cris L.; Chu, Hou Cheng; Ogawa, Nobuo; Inwood, William; Sementchenko, Victor; Beaton, Amy; Weiszmann, Richard; Celniker, Susan E.; Knowles, David W.; Gingeras, Tom; Speed, Terence P.; Eisen, Michael B.; Biggin, Mark D.

    2008-01-10

    Identifying the genomic regions bound by sequence-specific regulatory factors is central both to deciphering the complex DNA cis-regulatory code that controls transcription in metazoans and to determining the range of genes that shape animal morphogenesis. Here, we use whole-genome tiling arrays to map sequences bound in Drosophila melanogaster embryos by the six maternal and gap transcription factors that initiate anterior-posterior patterning. We find that these sequence-specific DNA binding proteins bind with quantitatively different specificities to highly overlapping sets of several thousand genomic regions in blastoderm embryos. Specific high- and moderate-affinity in vitro recognition sequences for each factor are enriched in bound regions. This enrichment, however, is not sufficient to explain the pattern of binding in vivo and varies in a context-dependent manner, demonstrating that higher-order rules must govern targeting of transcription factors. The more highly bound regions include all of the over forty well-characterized enhancers known to respond to these factors as well as several hundred putative new cis-regulatory modules clustered near developmental regulators and other genes with patterned expression at this stage of embryogenesis. The new targets include most of the microRNAs (miRNAs) transcribed in the blastoderm, as well as all major zygotically transcribed dorsal-ventral patterning genes, whose expression we show to be quantitatively modulated by anterior-posterior factors. In addition to these highly bound regions, there are several thousand regions that are reproducibly bound at lower levels. However, these poorly bound regions are, collectively, far more distant from genes transcribed in the blastoderm than highly bound regions; are preferentially found in protein-coding sequences; and are less conserved than highly bound regions. Together these observations suggest that many of these poorly-bound regions are not involved in early

  14. Identification and characterization of transcription networks in environmentally significant species

    SciTech Connect

    Lawrence, Charles E.; McCue, Lee Ann

    2005-11-30

    Understanding the regulation of gene expression, transcription regulation in particular, is one of the grand challenges of molecular biology. Transcription regulation is arguably the most important foundation of cellular function, since it exerts the most fundamental control of the abundance of virtually all of a cell's functional macromolecules. Nevertheless, this process, perhaps because of its difficulty, has been the subject of only a limited number of genomic level analyses. We have undertaken bioinformatics projects to address this issue by developing (1) a cross-species comparison method (i.e. phylogenetic footprinting) for the identification of transcription factor binding sites, (2) a Bayesian clustering method to identify regulons, (3) an improved scanning algorithm that uses a position weight matrix and several related species sequence data to locate transcription factor binding sites, and (4) a method to predict cognate binding sites for transcription factors of unknown specificity. These bioinformatics methods were developed using the model proteobacterium Escherichia coli, with further applications to the genomes of environmentally significant microbes (Rhodopseudomonas palustris, Shewanella oneidensis) in later years of the grant.

  15. Focus Series: Program Finds Community "Readiness" Is the Key...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Program Finds Community "Readiness" Is the Key to More Retrofits Focus Series: Program Finds Community "Readiness" Is the Key to More Retrofits Focus Series: Program Finds ...

  16. Department of Energy Helping Americans Find Alternative Fuel...

    Office of Environmental Management (EM)

    Helping Americans Find Alternative Fuel Stations Department of Energy Helping Americans Find ... to find fuel stations, post custom fueling location maps on their own websites, ...

  17. Portsmouth Site Treatment Plan Director's Final Findings and...

    Energy Saver

    Treatment Plan Director's Final Findings and Orders Portsmouth Site Treatment Plan Director's Final Findings and Orders Portsmouth Site Treatment Plan Director's Final Findings and ...

  18. Resources for Handling Transcripts | U.S. DOE Office of Science (SC)

    Office of Science (SC)

    Graduate Transcripts for Current Graduate Institution » Resources for Handling Transcripts DOE Office of Science Graduate Student Research (SCGSR) Program SCGSR Home Eligibility Benefits Participant Obligations How to Apply Identifying a Collaborating DOE Laboratory Scientist Research Proposal Guidelines Office of Science Priority Research Areas for SCGSR Program Letters of Support Graduate Transcripts for Current Graduate Institution Resources for Handling Transcripts Application Evaluation

  19. Energy Savings Performance Contracting-Savings Measurement and Verification Transcript 2-24-2011.doc

    Energy.gov [DOE]

    Energy Savings Performance Contracting-Savings Measurement and Verification Transcript 2-24-2011.doc

  20. Transcript of the Guidelines for Home Energy Professionals project Prezi presentation.

    Energy.gov [DOE]

    This document contains the transcript of the Guidelines for Home Energy Professionals project Prezi presentation.

  1. Electricity Advisory Committee Meeting, March 10, 2011- Meeting Minutes and Transcript

    Energy.gov [DOE]

    Meeting minutes and transcript for the March 10, 2011 meeting of the Electricity Advisory Committee.

  2. Transcript of May 25 Webinar: Tribal Business Structures for Financing Projects

    Office of Energy Efficiency and Renewable Energy (EERE)

    Transcript for the Tribal Energy and Economic Development Webinar: Tribal Business Structures for Financing Projects.

  3. Community Development Finance Institutions-Opportunities for Partnerships with Energy Efficiency Programs Transcript.doc

    Office of Energy Efficiency and Renewable Energy (EERE)

    Community Development Finance Institutions-Opportunities for Partnerships with Energy Efficiency Programs Transcript.doc

  4. Electricity Advisory Committee Meeting, January 24, 2014- Meeting Summary and Transcript

    Energy.gov [DOE]

    Meeting summary and transcript for the January 24, 2014 meeting of the Electricity Advisory Committee.

  5. Meeting of the Electricity Advisory Committee, June 1 & 2, 2016- Meeting Minutes and Transcripts

    Energy.gov [DOE]

    Meeting minutes and transcripts for the June 1 & 2, 2016 meeting of the Electricity Advisory Committee.

  6. Landfill aeration worldwide: Concepts, indications and findings

    SciTech Connect

    Ritzkowski, M.; Stegmann, R.

    2012-07-15

    Highlights: Black-Right-Pointing-Pointer Different landfill aeration concepts and accordant application areas are described. Black-Right-Pointing-Pointer Examples of full scale projects are provided for Europe, North-America and Asia. Black-Right-Pointing-Pointer Major project findings are summarised, including prospects and limitations. Black-Right-Pointing-Pointer Inconsistencies between laboratory and full scale results have been elaborated. Black-Right-Pointing-Pointer An explanatory approach in connection with the inconsistencies is provided. - Abstract: The creation of sustainable landfills is a fundamental goal in waste management worldwide. In this connection landfill aeration contributes towards an accelerated, controlled and sustainable conversion of conventional anaerobic landfills into a biological stabilized state associated with a minimised emission potential. The technology has been successfully applied to landfills in Europe, North America and Asia, following different strategies depending on the geographical region, the specific legislation and the available financial resources. Furthermore, methodologies for the incorporation of landfill aeration into the carbon trade mechanisms have been developed in recent years. This manuscript gives an overview on existing concepts for landfill aeration; their application ranges and specifications. For all of the described concepts examples from different countries worldwide are provided, including details regarding their potentials and limitations. Some of the most important findings from these aeration projects are summarised and future research needs have been identified. It becomes apparent that there is a great demand for a systematisation of the available results and implications in order to further develop and optimise this very promising technology. The IWWG (International Waste Working Group) Task Group 'Landfill Aeration' contributes towards the achievement of this goal.

  7. UDAC 2014 Findings and Recommendations | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    2014 Findings and Recommendations UDAC 2014 Findings and Recommendations UDAC 2014 Findings and Recommendations (162.67 KB) More Documents & Publications Minutes from December 16, 2013 UDAC Meeting UDAC 2014 Report UDAC Findings and Recommendations 2014

  8. UDAC Findings and Recommendations 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Findings and Recommendations 2014 UDAC Findings and Recommendations 2014 UDAC Findings and Recommendations 2014 (228.2 KB) More Documents & Publications Minutes from December 16, 2013 UDAC Meeting UDAC 2014 Report UDAC 2014 Findings and Recommendations

  9. Finding Nonoverlapping Substructures of a Sparse Matrix

    SciTech Connect

    Pinar, Ali; Vassilevska, Virginia

    2005-08-11

    Many applications of scientific computing rely on computations on sparse matrices. The design of efficient implementations of sparse matrix kernels is crucial for the overall efficiency of these applications. Due to the high compute-to-memory ratio and irregular memory access patterns, the performance of sparse matrix kernels is often far away from the peak performance on a modern processor. Alternative data structures have been proposed, which split the original matrix A into A{sub d} and A{sub s}, so that A{sub d} contains all dense blocks of a specified size in the matrix, and A{sub s} contains the remaining entries. This enables the use of dense matrix kernels on the entries of A{sub d} producing better memory performance. In this work, we study the problem of finding a maximum number of nonoverlapping dense blocks in a sparse matrix, which is previously not studied in the sparse matrix community. We show that the maximum nonoverlapping dense blocks problem is NP-complete by using a reduction from the maximum independent set problem on cubic planar graphs. We also propose a 2/3-approximation algorithm that runs in linear time in the number of nonzeros in the matrix. This extended abstract focuses on our results for 2x2 dense blocks. However we show that our results can be generalized to arbitrary sized dense blocks, and many other oriented substructures, which can be exploited to improve the memory performance of sparse matrix operations.

  10. Finding nonoverlapping substructures of a sparse matrix

    SciTech Connect

    Pinar, Ali; Vassilevska, Virginia

    2004-08-09

    Many applications of scientific computing rely on computations on sparse matrices, thus the design of efficient implementations of sparse matrix kernels is crucial for the overall efficiency of these applications. Due to the high compute-to-memory ratio and irregular memory access patterns, the performance of sparse matrix kernels is often far away from the peak performance on a modern processor. Alternative data structures have been proposed, which split the original matrix A into A{sub d} and A{sub s}, so that A{sub d} contains all dense blocks of a specified size in the matrix, and A{sub s} contains the remaining entries. This enables the use of dense matrix kernels on the entries of A{sub d} producing better memory performance. In this work, we study the problem of finding a maximum number of non overlapping rectangular dense blocks in a sparse matrix, which has not been studied in the sparse matrix community. We show that the maximum non overlapping dense blocks problem is NP-complete by using a reduction from the maximum independent set problem on cubic planar graphs. We also propose a 2/3-approximation algorithm for 2 times 2 blocks that runs in linear time in the number of nonzeros in the matrix. We discuss alternatives to rectangular blocks such as diagonal blocks and cross blocks and present complexity analysis and approximation algorithms.

  11. Nuclear sequestration of COL1A1 mRNA transcript associated with type I osteogenesis imperfecta (OI)

    SciTech Connect

    Primorac, D.; Stover, M.L.; McKinstry, M.B.

    1994-09-01

    Previously we identified an OI type I patient with a splice donor mutation that resulted in intron 26 retention instead of exon skipping and sequestration of normal levels of the mutant transcript in the nuclear compartment. Intron retention was consistent with the exon definition hypothesis for splice site selection since the size of the exon-intron-exon unit was less than 300 bp. Furthermore, the retained intron contained in-frame stop codons which is thought to cause the mutant RNA to remain within the nucleus rather than appearing in the cytoplasm. To test these hypotheses, genomic fragments containing the normal sequence or the donor mutation were cloned into a collagen minigene and expressed in stably tansfected NIH 3T3 cells. None of the modifications to the normal intron altered the level of RNA that accumulated in the cytoplasm, as expected. However none of the modifications to the mutant intron allowed accumulation of normal levels of mRNA in the cytoplasm. Moreover, in contrast to our findings in the patient`s cells only low levels of mutant transcript were found in the nucleus; a fraction of the transcript did appear in the cytoplasm which had spliced the mutant donor site correctly. Nuclear run-on experiments demonstrated equal levels of transcription from each transgene. Expression of another donor mutation known to cause in-frame exon skipping in OI type IV was accurately reproduced in the minigene in transfected 3T3 cells. Our experience suggests that either mechanism can lead to formation of a null allele possibly related to the type of splicing events surrounding the potential stop codons. Understanding the rules governing inactivation of a collagen RNA transcript may be important in designing a strategy to inactivate a dominate negative mutation associated with the more severe forms of OI.

  12. Occupancy by key transcription factors is a more accurate predictor of enhancer activity than histone modifications or chromatin accessibility

    SciTech Connect

    Dogan, Nergiz; Wu, Weisheng; Morrissey, Christapher S.; Chen, Kuan-Bei; Stonestrom, Aaron; Long, Maria; Keller, Cheryl A.; Cheng, Yong; Jain, Deepti; Visel, Axel; Pennacchio, Len A.; Weiss, Mitchell J.; Blobel, Gerd A.; Hardison, Ross C.

    2015-04-23

    Regulated gene expression controls organismal development, and variation in regulatory patterns has been implicated in complex traits. Thus accurate prediction of enhancers is important for further understanding of these processes. Genome-wide measurement of epigenetic features, such as histone modifications and occupancy by transcription factors, is improving enhancer predictions, but the contribution of these features to prediction accuracy is not known. Given the importance of the hematopoietic transcription factor TAL1 for erythroid gene activation, we predicted candidate enhancers based on genomic occupancy by TAL1 and measured their activity. Contributions of multiple features to enhancer prediction were evaluated based on the results of these and other studies. Results: TAL1-bound DNA segments were active enhancers at a high rate both in transient transfections of cultured cells (39 of 79, or 56%) and transgenic mice (43 of 66, or 65%). The level of binding signal for TAL1 or GATA1 did not help distinguish TAL1-bound DNA segments as active versus inactive enhancers, nor did the density of regulation-related histone modifications. A meta-analysis of results from this and other studies (273 tested predicted enhancers) showed that the presence of TAL1, GATA1, EP300, SMAD1, H3K4 methylation, H3K27ac, and CAGE tags at DNase hypersensitive sites gave the most accurate predictors of enhancer activity, with a success rate over 80% and a median threefold increase in activity. Chromatin accessibility assays and the histone modifications H3K4me1 and H3K27ac were sensitive for finding enhancers, but they have high false positive rates unless transcription factor occupancy is also included. Conclusions: Occupancy by key transcription factors such as TAL1, GATA1, SMAD1, and EP300, along with evidence of transcription, improves the accuracy of enhancer predictions based on epigenetic features.

  13. Occupancy by key transcription factors is a more accurate predictor of enhancer activity than histone modifications or chromatin accessibility

    DOE PAGES [OSTI]

    Dogan, Nergiz; Wu, Weisheng; Morrissey, Christapher S.; Chen, Kuan-Bei; Stonestrom, Aaron; Long, Maria; Keller, Cheryl A.; Cheng, Yong; Jain, Deepti; Visel, Axel; et al

    2015-04-23

    Regulated gene expression controls organismal development, and variation in regulatory patterns has been implicated in complex traits. Thus accurate prediction of enhancers is important for further understanding of these processes. Genome-wide measurement of epigenetic features, such as histone modifications and occupancy by transcription factors, is improving enhancer predictions, but the contribution of these features to prediction accuracy is not known. Given the importance of the hematopoietic transcription factor TAL1 for erythroid gene activation, we predicted candidate enhancers based on genomic occupancy by TAL1 and measured their activity. Contributions of multiple features to enhancer prediction were evaluated based on the resultsmore » of these and other studies. Results: TAL1-bound DNA segments were active enhancers at a high rate both in transient transfections of cultured cells (39 of 79, or 56%) and transgenic mice (43 of 66, or 65%). The level of binding signal for TAL1 or GATA1 did not help distinguish TAL1-bound DNA segments as active versus inactive enhancers, nor did the density of regulation-related histone modifications. A meta-analysis of results from this and other studies (273 tested predicted enhancers) showed that the presence of TAL1, GATA1, EP300, SMAD1, H3K4 methylation, H3K27ac, and CAGE tags at DNase hypersensitive sites gave the most accurate predictors of enhancer activity, with a success rate over 80% and a median threefold increase in activity. Chromatin accessibility assays and the histone modifications H3K4me1 and H3K27ac were sensitive for finding enhancers, but they have high false positive rates unless transcription factor occupancy is also included. Conclusions: Occupancy by key transcription factors such as TAL1, GATA1, SMAD1, and EP300, along with evidence of transcription, improves the accuracy of enhancer predictions based on epigenetic features.« less

  14. Russian research capabilities: Findings of site visits

    SciTech Connect

    Wester, D.W.

    1994-02-01

    In June 1993, a proposal was presented to the International Environmental Institute (IEI) in Kennewick, Washington, to establish cooperation and coordination to further pursue the interests of the United States of America and the Republic of Russia in the application and promotion of environmental technology; characterization, treatment, handling, isolation, and disposal of hazardous and radioactive materials; conversion of defense sites to other purposes; and technology transfer, cooperative programs, joint technology development and contractual research. In response to this proposal, IEI and Pacific Northwest Laboratory (PNL) jointly provided funding to send Dr. Dennis W. Wester on a fact-finding mission to Novosibirsk, Moscow, and St. Petersburg, Russia. The trip covered a period of eight weeks, six of which were spent in Novosibirsk and adjoining or related cities and one of which was spent in each of Moscow and St. Petersburg. The general objectives of the trip were to establish a basis for cooperation between IEI and the Russian Academy of Sciences (RAS) for future coordination of mutual interests and objectives such as technology acquisition, development, demonstration, application, and commercialization; use of capabilities and assets developed by the U.S. Department of Energy (DOE) and the RAS; and expediting of cooperative agreements, personnel exchanges, joint ventures and other contractual relationships. The particular objectives of this trip were to evaluate the capabilities of the RAS to satisfy the technology needs associated with the cleanup of the Hanford Site and similar sites in the U.S. and to evaluate the expediency of establishing an IEI presence in Russia.

  15. Trigger loop folding determines transcription rate of Escherichia coli’s RNA polymerase

    DOE PAGES [OSTI]

    Mejia, Yara X.; Nudler, Evgeny; Bustamante, Carlos

    2014-12-31

    Two components of the RNA polymerase (RNAP) catalytic center, the bridge helix and the trigger loop (TL), have been linked with changes in elongation rate and pausing. Here, single molecule experiments with the WT and two TL-tip mutants of the Escherichia coli enzyme reveal that tip mutations modulate RNAP’s pause-free velocity, identifying TL conformational changes as one of two rate-determining steps in elongation. Consistent with this observation, we find a direct correlation between helix propensity of the modified amino acid and pause-free velocity. Moreover, nucleotide analogs affect transcription rate, suggesting that their binding energy also influences TL folding. A kineticmore » model in which elongation occurs in two steps, TL folding on nucleoside triphosphate (NTP) binding followed by NTP incorporation/pyrophosphate release, quantitatively accounts for these results. The TL plays no role in pause recovery remaining unfolded during a pause. The model suggests a finely tuned mechanism that balances transcription speed and fidelity.« less

  16. Trigger loop folding determines transcription rate of Escherichia colis RNA polymerase

    SciTech Connect

    Mejia, Yara X.; Nudler, Evgeny; Bustamante, Carlos

    2014-12-31

    Two components of the RNA polymerase (RNAP) catalytic center, the bridge helix and the trigger loop (TL), have been linked with changes in elongation rate and pausing. Here, single molecule experiments with the WT and two TL-tip mutants of the Escherichia coli enzyme reveal that tip mutations modulate RNAPs pause-free velocity, identifying TL conformational changes as one of two rate-determining steps in elongation. Consistent with this observation, we find a direct correlation between helix propensity of the modified amino acid and pause-free velocity. Moreover, nucleotide analogs affect transcription rate, suggesting that their binding energy also influences TL folding. A kinetic model in which elongation occurs in two steps, TL folding on nucleoside triphosphate (NTP) binding followed by NTP incorporation/pyrophosphate release, quantitatively accounts for these results. The TL plays no role in pause recovery remaining unfolded during a pause. The model suggests a finely tuned mechanism that balances transcription speed and fidelity.

  17. EA-1922: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1922: Finding of No Significant Impact Combined Power and Biomass Heating System, Fort Yukon, Alaska Based on the EA, DOE finds that providng ...

  18. EA-1832: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    32: Finding of No Significant Impact EA-1832: Finding of No Significant Impact Rainer Biogas LLC Community Anaerobic Manure Digester, Enumclaw, King County, Washington The U.S....

  19. EMGeo: Risk Minimizing Software for Finding Offshore Fossil Fuels...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Find More Like This Return to Search EMGeo: Risk Minimizing Software for Finding Offshore ... developed advanced software for discovering and mapping offshore fossil fuel deposits. ...

  20. EA-1378: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    378: Finding of No Significant Impact EA-1378: Finding of No Significant Impact Site-Wide Environmental Assessment of National Renewable Energy Laboratory's National Wind ...

  1. EA-1938: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1938: Finding of No Significant Impact Grieve Unit CO2 Enhanced Recovery Project, Natrona County, WY Western has reviewed BLM's EA "Grieve...

  2. EA-1605: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1605: Finding of No Significant Impact Biomass Cogeneration and Heating Facilities at the Savannah River Site, South Carolina The Department of...

  3. EA-1784: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    4: Finding of No Significant Impact EA-1784: Finding of No Significant Impact Fotowatio Nevada Solar, LLC's APEX Solar Power Project in Clark County, Nevada The Department of...

  4. EA-1862: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1862: Finding of No Significant Impact Oneida Seven Generations Corporation: Energy Recovery Project, Green Bay, Wisconsin The U.S. Department...

  5. Innovative Financing Solutions: Finding Money for Your Energy...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Innovative Financing Solutions: Finding Money for Your Energy Efficiency Projects Innovative Financing Solutions: Finding Money for Your Energy Efficiency Projects Provides an ...

  6. EA-1785: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    5: Finding of No Significant Impact EA-1785: Finding of No Significant Impact Phase III Anthropogenic Test Characterization Project, Mobile County, Alabama Based on the analyses in...

  7. EA-1853: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    of No Significant Impact EA-1853: Finding of No Significant Impact Perrin Ranch Wind Energy Interconnection Project, Coconino County, Arizona Based on findings and analysis in...

  8. EA-1923: Mitigated Finding of No Significant Impact | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Mitigated Finding of No Significant Impact EA-1923: Mitigated Finding of No Significant Impact Green Energy School Wind Turbine Project on Saipan, Commonwealth of the Northern ...

  9. Jefferson Lab finds its man Mont (Inside Business) | Jefferson...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    https:www.jlab.orgnewsarticlesjefferson-lab-finds-its-man-mont-inside-business Jefferson Lab finds its man Mont Hugh Montgomery Hugh Montgomery, a British nuclear physicist...

  10. EA-1802: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    2: Finding of No Significant Impact EA-1802: Finding of No Significant Impact Chicago View Wind Project Chicago Heights, Cook County, Illinois The Department of Energy's Proposed...

  11. Community Wind Handbook/Find an Installer | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    * Submit Permit Applications * Find an Installer * Purchase Equipment * Plan for Maintenance Find an Installer Homeowners, ranchers, and small businesses can install wind...

  12. EIS-0372: Record of Decision and Floodplain Statement of Findings...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Record of Decision and Floodplain Statement of Findings EIS-0372: Record of Decision and Floodplain Statement of Findings Bangor Hydro-Electric Company Northeast Reliability...

  13. EIS-0456: Record of Decision and Floodplain Statement of Findings...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Record of Decision and Floodplain Statement of Findings EIS-0456: Record of Decision and Floodplain Statement of Findings Cushman Hydroelectric Project, Mason County, Washington...

  14. EA-1698: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    8: Finding of No Significant Impact EA-1698: Finding of No Significant Impact Baldwin Wind Energy Center Project Baldwin Wind, LLC has requested to interconnect their proposed new...

  15. EA-1807: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1807: Finding of No Significant Impact Heartland Community College Wind Energy Project, Normal, McLean County, Illinois The Department of...

  16. EA-1819: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1819: Finding of No Significant Impact Kilowatts for Kenston Wind Energy Project, Chagrin Falls, Geauga County, Ohio The Department of Energy's...

  17. EA-1829: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Final Environmental Assessment and Finding of No Significant Impact EA-1829: Final Environmental Assessment and Finding of No Significant Impact Phycal Algae Pilot Project, Wahiawa...

  18. EA-1866: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    6: Final Environmental Assessment and Finding of No Significant Impact EA-1866: Final Environmental Assessment and Finding of No Significant Impact Argonne National Laboratory...

  19. EA-1889: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Final Environmental Assessment and Finding of No Significant Impact EA-1889: Final Environmental Assessment and Finding of No Significant Impact Disposal of Decommissioned,...

  20. EA-1869: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    69: Final Environmental Assessment and Finding of No Significant Impact EA-1869: Final Environmental Assessment and Finding of No Significant Impact General Motors LLC, Electric...

  1. EA-1769: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    9: Final Environmental Assessment and Finding of No Significant Impact EA-1769: Final Environmental Assessment and Finding of No Significant Impact Houston Advanced Research...

  2. EA-1947: Draft Revised Finding of No Significant Impact | Department...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    1947: Draft Revised Finding of No Significant Impact EA-1947: Draft Revised Finding of No Significant Impact Transfer of the Kansas City Plant, Kansas City, Missouri The National ...

  3. EA-1733: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1733: Finding of No Significant Impact Calpine Enhanced Geothermal Systems Project, Sonoma County, California DOE determined that providing...

  4. EA-1839: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    39: Final Environmental Assessment and Finding of No Significant Impact EA-1839: Final Environmental Assessment and Finding of No Significant Impact Department of Energy Loan ...

  5. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact Silver Peak Area Geothermal Exploration Project, Esmeralda County, NV December 7, 2012 EA-1863: Finding of No Significant Impact Vegetation...

  6. EA-1984: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1984: Finding of No Significant Impact Disposition of Five Signature Properties at Idaho National Laboratory DOE has decided to implement the...

  7. Upcoming change to weekly crude oil inventory data series

    Gasoline and Diesel Fuel Update

    3 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration This is an unedited Transcript of the Fall Meeting of the Energy Information Administration and the American Statistical Association Committee on Energy Statistics. The meeting took place October 16 and 17, 2003 in the Forrestal Building at 1000 Independence Ave., S.W., Washington, D.C. 20585. Most of the sessions were in 8E-089, although several break out sessions were in

  8. Untitled Document

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Revision to the Analysis of the Impacts of the Clean Power Plan May 27, 2015 Minor changes to Table 7. Nuclear power projections in three cases

    3 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration This is an unedited Transcript of the Fall Meeting of the Energy Information Administration and the American Statistical Association Committee on Energy Statistics. The meeting took place October 16 and 17, 2003 in the Forrestal

  9. Untitled Document

    Energy Information Administration (EIA) (indexed site)

    3 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration This is an unedited Transcript of the Fall Meeting of the Energy Information Administration and the American Statistical Association Committee on Energy Statistics. The meeting took place October 16 and 17, 2003 in the Forrestal Building at 1000 Independence Ave., S.W., Washington, D.C. 20585. Most of the sessions were in 8E-089, although several break out sessions were in

  10. Transcript of Tribal Energy and Economic Development September Webinar:

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Strategic Partnerships for Clean Energy and Economic Development | Department of Energy September Webinar: Strategic Partnerships for Clean Energy and Economic Development Transcript of Tribal Energy and Economic Development September Webinar: Strategic Partnerships for Clean Energy and Economic Development Randy Manion: - wherever you may be and welcome to the ninth webinar of the 2016 DOE Tribal Renewable Energy Webinar series. Today's webinar is strategic partnerships for clean energy and

  11. Transcription factors for modification of lignin content in plants

    DOEpatents

    Wang, Huanzhong; Chen, Fang; Dixon, Richard A.

    2015-06-02

    The invention provides methods for modifying lignin, cellulose, xylan, and hemicellulose content in plants, and for achieving ectopic lignification and, for instance, secondary cell wall synthesis in pith cells, by altered regulation of a WRKY transcription factor. Nucleic acid constructs for altered WRKY-TF expression are described. Transgenic plants are provided that comprise modified pith cell walls, and lignin, cellulose, and hemicellulose content. Plants described herein may be used, for example, as improved biofuel feedstock and as highly digestible forage crops.

  12. Text Transcript of the Tribal Energy and Economic Development February

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Webinar: Exploring Your Energy Markets | Department of Energy Development February Webinar: Exploring Your Energy Markets Text Transcript of the Tribal Energy and Economic Development February Webinar: Exploring Your Energy Markets Randy Manion: Good morning or good afternoon wherever you may be and welcome to the second webinar of the 2016 DOE Tribal Renewable Energy Webinar Series: Exploring Your Energy Markets. I'm Randy Manion, today's webinar chair and manager of Western Area Power

  13. Text Transcript of the Tribal Energy and Economic Development March

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Webinar: Transmission and Grid Basics for Tribal Economic and Energy Development | Department of Energy Development March Webinar: Transmission and Grid Basics for Tribal Economic and Energy Development Text Transcript of the Tribal Energy and Economic Development March Webinar: Transmission and Grid Basics for Tribal Economic and Energy Development Sean Esterly: Good morning, or good afternoon, wherever you may be joining us from, and welcome to the third webinar of the 2016 Department of

  14. Meeting of the Electricity Advisory Committee, June 29-30, 2015- Meeting Minutes and Transcripts

    Office of Energy Efficiency and Renewable Energy (EERE)

    Meeting minutes and transcripts for the June 29-30, 2015 meeting of the Electric Advisory Committee.

  15. Transcript of Tribal Energy and Economic Development August Webinar: Project Regulatory Considerations

    Energy.gov [DOE]

    Transcript of the Tribal Energy and Economic Development webinar, "Project Regulatory Considerations," held on Aug. 31, 2016.

  16. Meeting of the Electricity Advisory Committee Meeting, March 26-27, 2015- Meeting Minutes and Transcripts

    Energy.gov [DOE]

    Meeting minutes and transcripts for the March 26-27, 2015 meeting of the Electricity Advisory Committee.

  17. Electricity Advisory Committee Meeting, March 12-13, 2014- Meeting Minutes and Transcripts

    Energy.gov [DOE]

    Meeting minutes and transcripts for the March 12-13, 2014 meeting of the Electricity Advisory Committee.

  18. Electricity Advisory Committee Meeting, June 11-12, 2012- Meeting Minutes and Transcripts

    Energy.gov [DOE]

    Meeting minutes and transcripts for the June 11-12, 2012 meeting of the Electricity Advisory Committee.

  19. Electricity Advisory Committee Meeting, March 5-6, 2012- Meeting Minutes and Transcripts

    Energy.gov [DOE]

    Meeting minutes and transcripts for the March 5-6, 2012 meeting of the Electricity Advisory Committee.

  20. Electricity Advisory Committee Meeting, October 15-16, 2012- Meeting Summaries and Transcripts

    Energy.gov [DOE]

    Meeting summaries and transcripts for the October 15-16, 2012 meeting of the Electricity Advisory Committee.

  1. Electricity Advisory Committee Meeting, September 24-25, 2014- Meeting Summaries and Transcripts

    Energy.gov [DOE]

    Meeting summaries and transcripts for the September 24-25, 2014 meeting of the Electricity Advisory Committee.

  2. Transcript of Tribal Energy and Economic Webinar: Energy Planning for Tribal Economic Development

    Office of Energy Efficiency and Renewable Energy (EERE)

    Transcript for the Tribal Energy and Economic Webinar: Energy Planning for Tribal Economic Development held on Jan. 27, 2016.

  3. Electricity Advisory Committee Meeting, June 16-17, 2014- Meeting Summaries and Transcripts

    Energy.gov [DOE]

    Meeting summaries and transcripts for the June 16-17, 2014 meeting of the Electricity Advisory Committee.

  4. Transcript of June 29 Webinar: Tribal Clean Energy for Sovereignty and Economic Development

    Office of Energy Efficiency and Renewable Energy (EERE)

    Transcript for the Office of Indian Energy June 29 webinar, Tribal Clean Energy for Sovereignty and Economic Development.

  5. Meeting of the Electricity Advisory Committee Meeting, September 29-30, 2015- Meeting Minutes and Transcripts

    Energy.gov [DOE]

    Meeting minutes and transcripts for the September 29-30, 2015 meeting of the Electric Advisory Committee.

  6. Meeting of the Electricity Advisory Committee, September 29-30, 2015- Meeting Minutes and Transcripts

    Office of Energy Efficiency and Renewable Energy (EERE)

    Meeting minutes and transcripts for the September 29-30, 2015 meeting of the Electric Advisory Committee.

  7. Find your scientific document; then quickly find "more like this" at

    Office of Scientific and Technical Information (OSTI)

    Information Bridge | OSTI, US Dept of Energy Office of Scientific and Technical Information your scientific document; then quickly find "more like this" at Information Bridge Back to the OSTI News Listing for 2010 The "go to" site for Department of Energy full-text research documents has added a ''more like this" feature on the search results page. Now you can search for technical reports on your topics of interest and quickly locate related documents. The "more

  8. Transcriptional control in Alicyclobacillus acidocaldarius and associated genes, proteins, and methods

    SciTech Connect

    Lee, Brady D.; Thompson, David N.; Apel, William A.; Thompson, Vicki S.; Reed, David W.; Lacey, Jeffrey A.

    2015-11-17

    Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

  9. Transcriptional control in Alicyclobacillus acidocaldarius and associated genes, proteins, and methods

    DOEpatents

    Lee, Brady Deneys; Thompson, David N; Apel, William A.; Thompson, Vicki Slavchev; Reed, David W; Lacey, Jeffrey A

    2014-05-06

    Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

  10. Floodplain Assessment and Statement of Findings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    and Statement of Findings Floodplain Assessment and Statement of Findings Floodplain Assessment and Statement of Findings for Installation of a Renewable Energy Anaerobic Digester Facility at the University of California, Davis in Yolo County, California. Statement of Findings (450.47 KB) More Documents & Publications Floodplain Assessment for Installation of a Renewable Energy Anaerobic Digester Facility

  11. The transcriptional diversity of 25 Drosophila cell lines

    SciTech Connect

    Cherbas, Lucy; Willingham, Aarron; Zhang, Dayu; Yang, Li; Zou, Yi; Eads, Brian D.; Carlson, Joseph W.; Landolin, Jane M.; Kapranov, Philipp; Dumais, Jacqueline; Samsonova, Anastasia; Choi, Jeong-Hyeon; Roberts, Johnny; Davis, Carrie A.; Tang, Haixu; van Baren, Marijke J.; Ghosh, Srinka; Dobin, Alexander; Bell, Kim; Lin, Wei; Langton, Laura; Duff, Michael O.; Tenney, Aaron E.; Zaleski, Chris; Brent, Michael R.; Hoskins, Roger A.; Kaufman, Thomas C.; Andrews, Justen; Graveley, Brenton R.; Perrimon, Norbert; Celniker, Susan E.; Gingeras, Thomas R.; Cherbas, Peter

    2010-11-15

    Drosophila melanogaster cell lines are important resources for cell biologists. Here, we catalog the expression of exons, genes, and unannotated transcriptional signals for 25 lines. Unannotated transcription is substantial (typically 19% of euchromatic signal). Conservatively, we identify 1405 novel transcribed regions; 684 of these appear to be new exons of neighboring, often distant, genes. Sixty-four percent of genes are expressed detectably in at least one line, but only 21% are detected in all lines. Each cell line expresses, on average, 5885 genes, including a common set of 3109. Expression levels vary over several orders of magnitude. Major signaling pathways are well represented: most differentiation pathways are off and survival/growth pathways on. Roughly 50% of the genes expressed by each line are not part of the common set, and these show considerable individuality. Thirty-one percent are expressed at a higher level in at least one cell line than in any single developmental stage, suggesting that each line is enriched for genes characteristic of small sets of cells. Most remarkable is that imaginal discderived lines can generally be assigned, on the basis of expression, to small territories within developing discs. These mappings reveal unexpected stability of even fine-grained spatial determination. No two cell lines show identical transcription factor expression. We conclude that each line has retained features of an individual founder cell superimposed on a common cell line gene expression pattern. Wereport the transcriptional profiles of 25 Drosophila melanogaster cell lines, principally by whole-genome tiling microarray analysis of total RNA, carried out as part of the modENCODE project. The data produced in this study add to our knowledge of the cell lines and of the Drosophila transcriptome in several ways. We summarize the expression of previously annotated genes in each of the 25 lines with emphasis on what those patterns reveal

  12. Microsoft Word - Consolidated Final Transcript of CBS Tempe AZ

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    9 CONSENT-BASED SITING PUBLIC MEETING Phoenix Marriott Tempe at the Buttes 2000 W Westcourt Way Tempe, AZ 85282 June 23, 2016 FULL TRANSCRIPT Mr. Jim Hamilton. Good afternoon. And for those joining in a later time zone, good evening. Welcome to Tempe and to the sixth in a series of public meetings the Department is hosting on its Consent-Based Siting Program. Thank you all for being here today. My name is Jim Hamilton - I'm an advisor to the Department as part of their Consent-Based Siting Team

  13. Microsoft Word - Consolidated Meeting Denver Verbatim Transcript_Final

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Embassy Suites Denver - Stapleton 4444 Havana Street Denver, CO 80239 May 24, 2016 FULL TRANSCRIPT Mr. Jim Hamilton. Good afternoon. And for those in a later time zone via webinar, good evening. Welcome to Denver and to the fourth in a series of public meetings the Department is hosting on its Consent-Based Siting Program. Move in if you want - you don't have to sit in the periphery if you don't want to. We want to try to make it a little more intimate. Thank you for being here today. My name is

  14. Determining physical constraints in transcriptional initiationcomplexes using DNA sequence analysis

    SciTech Connect

    Shultzaberger, Ryan K.; Chiang, Derek Y.; Moses, Alan M.; Eisen,Michael B.

    2007-07-01

    Eukaryotic gene expression is often under the control ofcooperatively acting transcription factors whose binding is limited bystructural constraints. By determining these structural constraints, wecan understand the "rules" that define functional cooperativity.Conversely, by understanding the rules of binding, we can inferstructural characteristics. We have developed an information theory basedmethod for approximating the physical limitations of cooperativeinteractions by comparing sequence analysis to microarray expressiondata. When applied to the coordinated binding of the sulfur amino acidregulatory protein Met4 by Cbf1 and Met31, we were able to create acombinatorial model that can correctly identify Met4 regulatedgenes.

  15. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Project, Zachary, Louisiana November 24, 2010 EA-1797: Finding of No Significant Impact Agua Caliente Solar Project in Yuma County, Arizona November 19, 2010 EA-1727: Finding of No...

  16. EA-1726: Finding of No Significant Impact | Department of Energy

    Energy Saver

    Finding of No Significant Impact EA-1726: Finding of No Significant Impact Kahuku Wind Power, LLC, Construction of the Kahuku Wind Power Facility in Kahuku, O'ahu, Hawaii The ...

  17. Accident Investigation Board (AIB) findings about the drum breach...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Accident Investigation Board findings Accident Investigation Board (AIB) findings about the drum breach at WIPP WHEN: Apr 23, 2015 5:30 PM - 7:00 PM WHERE: Fuller Lodge 2132 ...

  18. STEM Volunteer Training: Types of Volunteering and Where to Find...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Types of Volunteering and Where to Find Opportunities STEM Volunteer Training: Types of Volunteering and Where to Find Opportunities April 14, 2016 3:00PM to 4:00PM EDT

  19. EERE 2014 Wind Technologies Market Report Finds Wind Power at...

    Energy Saver

    2014 Wind Technologies Market Report Finds Wind Power at Record Low Prices EERE 2014 Wind Technologies Market Report Finds Wind Power at Record Low Prices August 10, 2015 - 11:00am ...

  20. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    County, Nevada June 9, 2011 EA-1770: Finding of No Significant Impact Photovoltaic Solar Project at the Durango, Colorado Site June 3, 2011 EA-1858: Finding of No Significant...

  1. Commercial Building Energy Asset Score: Pilot Findings and Program...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Commercial Building Energy Asset Score: Pilot Findings and Program Update The webinar was held on April 16, 2014, to share the findings from the 2013 Pilot and provide a program ...

  2. EA-1917: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1917: Finding of No Significant Impact Wave Energy Test Facility Project, Newport, OR Based on the information presented in the Final EA, (DOE...

  3. Show Me the Money!: How to Find Financing, Access Incentives...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Show Me the Money: How to Find Financing, Access Incentives, and Get Projects Done Show Me the Money: How to Find Financing, Access Incentives, and Get Projects Done December 6, ...

  4. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    2012 EA-1812: Finding of No Significant Impact Haxtun Wind Energy Project, Logan and Phillips Counties, CO January 3, 2012 EA-1606: Finding of No Significant Impact Proposed Use...

  5. EA-1656: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1656: Finding of No Significant Impact MARET Center at Crowder College, Neosho, Missouri The U.S. Department of Energy is preparing an...

  6. EA-1894: Finding of No Significant Impact | Department of Energy

    Energy.gov [DOE] (indexed site)

    Albeni Falls Flexible Winter Lake Operations, Bonner, Idaho As a result of the analysis in this EA, I have the following findings. I find that: 1) the FWPO is not a substantial...

  7. Title 16 USC 1600 Congressional Findings | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    600 Congressional Findings Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- StatuteStatute: Title 16 USC 1600 Congressional FindingsLegal Abstract...

  8. Rainbows and Leprechauns: Finding Gold in Partnerships (101)...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Rainbows and Leprechauns: Finding Gold in Partnerships (101) Rainbows and Leprechauns: Finding Gold in Partnerships (101) March 17, 2016 1:00PM to 2:30PM EDT

  9. EA-1809: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1809: Finding of No Significant Impact White Earth Nation Wind Energy Project II, Becker and Mahnomen Counties, MN DOE's Golden Field Office has...

  10. EA-1933: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1933: Finding of No Significant Impact Yakama Nation Drop 4 Hydropower Project, Yakama Nation Reservation, WA Based on the Final EA, DOE has...

  11. The World's Largest Solar Project Finds a Home in California...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Solar Project Finds a Home in California The World's Largest Solar Project Finds a Home in ... That's the impact that the Blythe Solar Power Project is projected to have upon ...

  12. Got a Minute? Which Higgs did we find?

    SciTech Connect

    Stupak, John

    2014-06-26

    Dr. John Stupak talks about the discovery of the Higgs boson. Did scientists find the Higgs boson predicted back in 1964 or did they find just one of a group of particles, with the others still to be found?

  13. EA-1904: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    EA-1904: Finding of No Significant Impact Linac Coherent Light Source II at Stanford Linear Accelerator Laboratory, San Mateo, CA Based on the findings in the EA, DOE has...

  14. ULTRA-DEEP WATER ADVISORY COMMITTEE FINDINGS AND

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    ULTRA-DEEP WATER ADVISORY COMMITTEE FINDINGS AND RECOMMENDATIONS 2014 ULTRA-DEEPWATER ADVISORY COMMITTEE COMMITTEE FINDINGS AND RECOMMENDATIONS i Table of Contents Research and Development Program Committee ............................................ 1 Sunset Committee .................................................................................................. 5 Advisory Committee Members ........................................................................... 7 ULTRA-DEEPWATER

  15. Got a Minute? Which Higgs did we find?

    ScienceCinema

    Stupak, John

    2014-09-15

    Dr. John Stupak talks about the discovery of the Higgs boson. Did scientists find the Higgs boson predicted back in 1964 or did they find just one of a group of particles, with the others still to be found?

  16. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    in Kahuku, O'ahu, Hawaii May 18, 2010 EA-1699: Finding of No Significant Impact PopeDouglas Third Combustor Expansion Project, Alexandria, Minnesota May 7, 2010 EA-1635: Finding...

  17. EIS-0365: Record of Decision and Floodplain Statement of Findings...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Record of Decision and Floodplain Statement of Findings EIS-0365: Record of Decision and Floodplain Statement of Findings Imperial-Mexicali 230-kV Transmission Lines DOE announces...

  18. EA-1767: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    7: Finding of No Significant Impact EA-1767: Finding of No Significant Impact Virginia State Energy Program's Cephas C&D Wastes Biomass Project, Richmond, Virginia Based on the...

  19. EA-1814: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1814: Finding of No Significant Impact City of Montpelier Combined Heat and Power, and District Energy System, Montpelier, Vermont The U.S....

  20. EA-1665: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    5: Final Environmental Assessment and Finding of No Significant Impact EA-1665: Final Environmental Assessment and Finding of No Significant Impact Davis-Kingman Tap 69-kV...

  1. EA-1947: Finding of No Significant Impact | Department of Energy

    Energy Saver

    7: Finding of No Significant Impact EA-1947: Finding of No Significant Impact Transfer of ... and NNSA is issuing this FONSI. EA-1947FONSI-2013.pdf (169.65 KB) More Documents ...

  2. EA-1833: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1833: Finding of No Significant Impact The City of El Dorado Wind Energy Project, El Dorado, Butler County, Kansas The U.S. Department of...

  3. EA-1928: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1928: Finding of No Significant Impact White-Tailed Deer Management at Brookhaven National Lab, Upton, New York Based on the information in the...

  4. Finding and Winning Simplified Acquisitions, Part 1 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding and Winning Simplified Acquisitions, Part 1 Finding and Winning Simplified Acquisitions, Part 1 Presented at the 15th Annual DOE Small Business Forum & Expo by: Guy Timberlake, Chief Visionary Officer and Chief Executive Officer, The American Small Business Coalition Finding and Winning Simplified Acquisitions, Part 1 (4.41 MB) More Documents & Publications Finding and Winning Simplified Acquisitions, Part 2 Chapter 17 - Special Contracting Methods Interagency Acquisition

  5. Finding and Winning Simplified Acquisitions, Part 2 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding and Winning Simplified Acquisitions, Part 2 Finding and Winning Simplified Acquisitions, Part 2 Presented at the 15th Annual DOE Small Business Forum & Expo by: Guy Timberlake, Chief Visionary Officer and Chief Executive Officer, The American Small Business Coalition Finding and Winning Simplified Acquisitions, Part 2 (6.47 MB) More Documents & Publications Finding and Winning Simplified Acquisitions, Part 1 December 12, 2013 Business Opportunity Session Presentations Acquisition

  6. EIS-0369: Floodplain Statement of Finding | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    9: Floodplain Statement of Finding EIS-0369: Floodplain Statement of Finding Rail Alignment for the Construction and Operation of a Railroad in Nevada to a Geologic Repository at Yucca Mountain, Nye County, Nevada Floodplain Statement of Findings for the Final Environmental Impact Statement for a Rail Alignment for the Construction and Operation of a Railroad in Nevada to a Geologic Repository at Yucca Mountain, Nye County, Nevada Floodplain Statement of Findings for the Final Environmental

  7. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Findings of No Significant Impact (FONSI) Findings of No Significant Impact (FONSI) Findings of No Significant Impact are public documents issued by a Federal agency briefly presenting the reasons why an action for which the agency has prepared an environmental assessment will not have a significant effect on the human environment and, therefore, will not require preparation of an environmental impact statement. If you have any trouble finding a specific document, please contact

  8. Comparative genomics of transcriptional regulation of methionine metabolism in proteobacteria

    SciTech Connect

    Leyn, Semen A.; Suvorova, Inna A.; Kholina, Tatiana D.; Sherstneva, Sofia S.; Novichkov, Pavel S.; Gelfand, Mikhail S.; Rodionov, Dmitry A.; Kuipers, Oscar P.

    2014-11-20

    Methionine metabolism and uptake genes in Proteobacteria are controlled by a variety of RNA and DNA regulatory systems. We have applied comparative genomics to reconstruct regulons for three known transcription factors, MetJ, MetR, and SahR, and three known riboswitch motifs, SAH, SAM-SAH, and SAM_alpha, in ~200 genomes from 22 taxonomic groups of Proteobacteria. We also identified two novel regulons: a SahR-like transcription factor SamR controlling various methionine biosynthesis genes in the Xanthomonadales group, and a potential RNA regulatory element with terminator-antiterminator mechanism controlling the metX or metZ genes in beta-proteobacteria. For each analyzed regulator we identified the core, taxon-specific and genome-specific regulon members. By analyzing the distribution of these regulators in bacterial genomes and by comparing their regulon contents we elucidated possible evolutionary scenarios for the regulation of the methionine metabolism genes in Proteobacteria.

  9. Comparative genomics of transcriptional regulation of methionine metabolism in proteobacteria

    DOE PAGES [OSTI]

    Leyn, Semen A.; Suvorova, Inna A.; Kholina, Tatiana D.; Sherstneva, Sofia S.; Novichkov, Pavel S.; Gelfand, Mikhail S.; Rodionov, Dmitry A.; Kuipers, Oscar P.

    2014-11-20

    Methionine metabolism and uptake genes in Proteobacteria are controlled by a variety of RNA and DNA regulatory systems. We have applied comparative genomics to reconstruct regulons for three known transcription factors, MetJ, MetR, and SahR, and three known riboswitch motifs, SAH, SAM-SAH, and SAM_alpha, in ~200 genomes from 22 taxonomic groups of Proteobacteria. We also identified two novel regulons: a SahR-like transcription factor SamR controlling various methionine biosynthesis genes in the Xanthomonadales group, and a potential RNA regulatory element with terminator-antiterminator mechanism controlling the metX or metZ genes in beta-proteobacteria. For each analyzed regulator we identified the core, taxon-specific andmore » genome-specific regulon members. By analyzing the distribution of these regulators in bacterial genomes and by comparing their regulon contents we elucidated possible evolutionary scenarios for the regulation of the methionine metabolism genes in Proteobacteria.« less

  10. How to Find Ames Laboratory | The Ames Laboratory

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    How to Find Ames Laboratory Finding the Ames Laboratory The Ames Laboratory has it's main administrative offices in the Technical and Administrative Services Facility (TASF) building on the Iowa State University Campus. Technical and Administrative Services Facility (TASF) 2408 Pammel Drive, Ames, IA 50011-1015 For directions from the Des Moines Airport please use the link with the following directions: https://goo.gl/maps/c6SOK Finding the Ames Laboratory on the ISU Campus Image Click on the

  11. Alternative Fuels Data Center: Minnesota School District Finds Cost

    Alternative Fuels and Advanced Vehicles Data Center

    Savings, Cold-Weather Reliability with Propane Buses Minnesota School District Finds Cost Savings, Cold-Weather Reliability with Propane Buses to someone by E-mail Share Alternative Fuels Data Center: Minnesota School District Finds Cost Savings, Cold-Weather Reliability with Propane Buses on Facebook Tweet about Alternative Fuels Data Center: Minnesota School District Finds Cost Savings, Cold-Weather Reliability with Propane Buses on Twitter Bookmark Alternative Fuels Data Center: Minnesota

  12. Find Funding | U.S. DOE Office of Science (SC)

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Find Funding Funding Opportunities Funding Opportunities Home Grants & Contracts Support Award Search / Public Abstracts Find Funding Early Career Research Program Statement on Digital Data Management Acknowledgements of Federal Support Contact Information Office of Science U.S. Department of Energy 1000 Independence Ave., SW Washington, DC 20585 P: (202) 586-5430 Find Funding Print Text Size: A A A FeedbackShare Page Additional information on funding opportunities, including program

  13. EA-1495: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    5: Finding of No Significant Impact EA-1495: Finding of No Significant Impact USEC Inc. American Centrifuge Lead Cascade Facility at Piketon, Ohio The purpose of NRC's proposed action is to authorize possession and use of source and special nuclear material at the Lead Cascade in Piketon, Ohio. Finding of No Significant Impact for the United States Enrichment Corporation Incorporated, American Centrifuge Lead Cascade Facility at Piketon, Ohio (369.61 KB) More Documents & Publications

  14. Portsmouth Integration Director's Final Findings and Order | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Energy Integration Director's Final Findings and Order Portsmouth Integration Director's Final Findings and Order Portsmouth Integration Director's Final Findings and Order purpose is to: integrate the on-site work required for specific units to avoid duplication of effort, and efficiently perform sitewide ground water monitoring and surveillance and maintenance activities; recognize that a substantial portion of the tasks required under existing approved closure plans for certain units have

  15. FOIA-Summary of Findings: Circumstances surrounding decisions regarding the

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    security clearance, access and work assignments | Department of Energy FOIA-Summary of Findings: Circumstances surrounding decisions regarding the security clearance, access and work assignments FOIA-Summary of Findings: Circumstances surrounding decisions regarding the security clearance, access and work assignments FOIA request FOIA-Summary of Findings: Circumstances surrounding decisions regarding the security clearance, access and work assignments (4.61 MB) More Documents &

  16. EA-2010: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-2010: Finding of No Significant Impact Alternating Gradient Synchrotron Complex Improvements at Brookhaven National Laboratory; Upton, New York DOE issued a Finding of No Significant Impact for the proposed activities at the Brookhaven National Laboratory Alternating Gradient Synchrotron Complex, including upgrading the Accelerator Test Facility to 10 GeV by adding laser acceleration, upgrading the Linac proton beam current, upgrading the facilities to produce

  17. EA-2018: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-2018: Finding of No Significant Impact EA-2018: Front Range-Midway Solar Interconnection Project; El Paso County, Colorado Western Area Power Administration issued a Finding of No Significant Impact on September 21, 2016, for a proposal to interconnect a proposed photovoltaic solar facility adjacent to Western's existing Midway Substation in El Paso County, Colorado. For more information on this project, see: www.energy.gov/node/1907551. DOWNLOAD DOCUMENT

  18. EA-2024: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    4: Finding of No Significant Impact EA-2024: Finding of No Significant Impact Gap Material Plutonium - Transport, Receipt, and Processing Based on the analysis in the environmental assessment, the U.S. Department of Energy, National Nuclear Security Administration (NNSA), issued this finding of no significant impact for transporting up to 900 kilograms of plutonium from foreign countries to the Savannah River Site in South Carolina for storage and processing pending final disposition. NNSA will

  19. EA-1440: Finding of No Signficant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    : Finding of No Signficant Impact EA-1440: Finding of No Signficant Impact National Renewable Energy Laboratory's South Table Mountain Complex The Proposed Action is to operate the STM complex for alternative energy research with new and improved capabilities to support DOE"s mission to research, develop and transfer to industry renewable energy technologies. Finding of No Signficant Impact for the Site-Wide Environmental Assessment of the National Renewable Energy Laboratory's South Table

  20. EA-1797: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    7: Finding of No Significant Impact EA-1797: Finding of No Significant Impact Agua Caliente Solar Project in Yuma County, Arizona The Department of Energy has conducted an environmental assessment that analyzed the reasonably foreseeable environmental impacts associated with the 290 Megawatt gross output photovoltaic solar power project and associated interconnection transmission line proposed by Agua Caliente Solar, LLC in Yuma County, Arizona. Finding of No Significant Impact Department of

  1. EA-1406: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    06: Finding of No Significant Impact EA-1406: Finding of No Significant Impact Ground Water Compliance at the New Rifle, Colorado, UMTRA Project Site On the basis of the Environmental Assessment. which analyzed relevant environmental issues and addressed the concerns of stakeholders, DOE finds that no significant impact will result from implementing the planned action at the New Rifle site. This FONSI is documented pursuant to NEPA, the Council on Environmental Quality's "Regulations for

  2. Find Other Sources of EERE Funding | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Find Funding » Find Other Sources of EERE Funding Find Other Sources of EERE Funding Many financial opportunities are available besides EERE funding opportunity announcements (FOAs). Like FOAs, some are competitive, while others are non-competitive. Cooperative Research and Development Agreements (CRADAs) Formula Grants Acquisition Small Business Innovation Research (SBIR)/Small Business Technology Transfer (STTR) Awards Technology Investment Agreement (TIA) Unsolicited Proposals Cooperative

  3. Webinar Series: Presenting SEEDS Findings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Webinar Series: Presenting SEEDS Findings Webinar Series: Presenting SEEDS Findings The SunShot Solar Energy Evolution and Diffusion Studies (SEEDS) program investigates strategies for accelerating the pace of change for solar energy technologies as they are developed and deployed. In a series of three webinars, the National Renewable Energy Laboratory SEEDS research team discusses the findings and outcomes of their project. Date June 2016 Topic Financing, Incentives & Market Analysis

  4. EA-1770: Finding of No Significant Impact | Department of Energy

    Energy.gov [DOE] (indexed site)

    of the National Environmental Policy Act (NEPA). Finding of No Significant Impact Photovoltaic Solar Project at the Durango, Colorado, Disposal Site, La Plata County, DOEEA 1770...

  5. EA-1533: Finding of No Significant Impact | Department of Energy

    Energy Saver

    EA-1533: Finding of No Significant Impact Proposed Gas Main and Distribution System Upgrade, Pantex Plant, Amarillo, Texas This environmental assessment (EA) provides sufficient ...

  6. EA-1907: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Impact EA-1907: Finding of No Significant Impact Western Plains Energy, LLC Biogas Anaerobic Digester Facility, Oakley, Kansas Based on the analysis in the Final EA and...

  7. EA-1193: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    EA-1193: Finding of No Significant Impact Atmospheric Radiation Measurement (ARM) Program North Slope of Alaska and Adjacent Arctic Ocean Cloud and Radiation Testbed (CART) Site The ...

  8. EA-1762: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    of No Significant Impact EA-1762: Finding of No Significant Impact Wellford Landfill Methane and Greenhouse Gas to Energy Project, Spartanburg County, South Carolina The...

  9. PPPL physicists find clue to formation of magnetic fields around...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    results, in the range in which they are done, strongly suggest that such dynamo action is possible." These findings might lead to greater understanding of the behavior of...

  10. EA-1685: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    of No Significant Impact EA-1685: Finding of No Significant Impact Experimental Coal Flow Program at Clemson University The Western Area Power Administration (Western) is the...

  11. DOE Issues Finding of No Significant Impact and Final Environmental...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    DOE Issues Finding of No Significant Impact and Final Environmental Assessment on Road ... impacts of building an internal road to safely ship radioactive material within ...

  12. Floodplain and Wetland Assessment and Statement of Findings

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    of Findings for Construction of a Second Full Service Access Road over Lena Gulch from South Golden Road to the National Renewable Energy Laboratory's South Table Mountain ...

  13. Finding of No Significant Impact, Federal Register, 73 FR 2017...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    73 FR 2017, January 11, 2008 Document details the finding of no significant impact of the Energy-Efficient Performance Requirements for New Federal Commercial and Residential...

  14. EA-2022: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Western issued a finding of no significant impact for the proposal. The new hydropower generator would interconnect to Western's transmission system at an existing transmission ...

  15. EA-1183: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    EA-1183: Finding of No Significant Impact Coal-fired Diesel Generator University of ... the performance of a coal-fired generator at the University of Alaska, Fairbanks, ...

  16. CLASS DEVIATION FINDINGS AND DETERMINATION TO IMPLEMENT AN APPROPRIATI...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    PROVISION RELATED TO INTERNAL CONFIDENTIALITY AGREEMENTS AND THE REPORTING OF FRAUD, WASTE, AND ABUSE Findings I. Section 743 of Division E, Title VII of the Consolidated...

  17. EA-0921: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    EA-0921: Finding of No Significant Impact Ambulatory Research and Education Center, Oregon Health ... the meaning of NEPA. Therefore, an environmental impact statement is not required. ...

  18. EA-1801: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    EA-1801: Finding of No Significant Impact Granite Reliable Power Wind Project, Coos County, New Hampshire The Department of Energy is adopting an environmental assessment completed ...

  19. EA-1700: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    LBNLUniversity of California Berkeley programs that focus on computational and computer science research in a new facility on the LBNL site. PDF icon Finding of No Significant...

  20. Finding Large Aperture Fractures in Geothermal Resource Areas...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    ...-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Finding Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component Long-Offset Surface Seismic ...

  1. Findings of No Significant Impact (FONSI) | Department of Energy

    Energy.gov [DOE] (indexed site)

    Ridge Reservation for the biological control of the emerald ash borer, a nonnative invasive beetle. May 1, 2015 EA-1796: Finding of No Significant Impact Loan Guarantee to...

  2. EA-1747: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    EA-1747: Finding of No Significant Impact Rocky Flats Surface Water Configuration, Jefferson County, Colorado Surface Water Configuration Project at the Rocky Flats Site, Colorado ...

  3. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    EA-1754: Finding of No Significant Impact Public Service Company of New Mexico, Photovoltaic Plus Battery for Simultaneous Voltage Smoothing and Peak Shifting Project,...

  4. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Uranium July 1, 2009 EA-1645: Finding of No Significant Impact Loan Guarantee for Sage Electrochromics SageGlass High Volume Manufacturing Facility in Faribault, MN June 26,...

  5. Commercial Building Energy Asset Score: Pilot Findings and Program Update

    Energy.gov [DOE]

    The webinar was held on April 16, 2014, to share the findings from the 2013 Pilot and provide a program update on the Commercial Building Energy Asset Score.

  6. Rainbows and Leprechauns: Finding Gold in Partnerships (101)...

    Energy.gov [DOE] (indexed site)

    Peer Exchange Call Series: Rainbows and Leprechauns: Finding Gold in Partnerships (101), March 17, 2016, call slides and discussion summary. Call Slides and Discussion Summary ...

  7. EA-1426: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    EA-1426: Finding of No Significant Impact Linac Coherent Light Source Project, Stanford ... Coherent Light Source (LCLS) at the Stanford Linear Accelerator Center (SLAC), ...

  8. EA-1927: Finding of No Significant Impact | Department of Energy

    Energy Saver

    EA-1927: Finding of No Significant Impact Paducah Gaseous Diffusion Plant Potential Land ... the Paducah Gaseous Diffusion Plant from DOE to other entities for economic development. ...

  9. Mound Plant Director's Final Findings and Orders, October 4,...

    Office of Environmental Management (EM)

    Director's Final Findings and Orders State Ohio Agreement Type Compliance Agreement Legal Driver(s) FFCAct Scope Summary Approve the Compliance Plan Volume of the amended PSTP, ...

  10. EA-1777: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Significant Impact EA-1777: Finding of No Significant Impact Lincoln Electric's Wind Energy Project in Euclid, OH DOE prepared an environmental assessment analyzing the potential...

  11. EA-1719: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Impact EA-1719: Finding of No Significant Impact Novolyte Technologies, Inc. Electric Drive Vehicle Battery and Component Manufacturing Initiative Project, Zachary, Louisiana DOE...

  12. FOIA-Summary of Findings: Circumstances surrounding decisions...

    Energy.gov [DOE] (indexed site)

    FOIA request FOIA-Summary of Findings: Circumstances surrounding decisions regarding the security clearance, access and work assignments (4.61 MB) More Documents & Publications ...

  13. EA-1535: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    EA-1535: Finding of No Significant Impact Uranium Leasing Program U.S. Department of Energy Office of Legacy Management prepared the Uranium Leasing Program Programmatic ...

  14. Oregon 401 Evaluation and Findings Checklist | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    401 Evaluation and Findings Checklist Jump to: navigation, search OpenEI Reference LibraryAdd to library PermittingRegulatory Guidance - Checklist: Oregon 401 Evaluation and...

  15. EA-1796: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    near Gillespie, Arizona, DOEEA-1796 (February 2011) More Documents & Publications EA-1796: Final Environmental Assessment EA-1796: Finding of No Significant Impact MESQUITE

  16. EA-1952: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    ...energy.govnode607866. Download Document PDF icon EA-1952: Finding of No Significant Impact More Documents & Publications EA-1952: Draft Environmental Assessment EA-1952: Final

  17. EA-1931: Finding of No Significant Impact and Mitigation Action...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Bonneville Power Administration issued a finding of no significant impact and a ... More Documents & Publications EA-1931: Draft Environmental Assessment EA-1931: Final ...

  18. EA-1979: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    icon EA-1979-FONSI-2015.pdf More Documents & Publications EA-1979: Draft Environmental Assessment EA-1979: Final Environmental Assessment EA-1955: Finding of No Significant Impact

  19. EA-1836: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    to facilitiate installation and operations of a high-efficiency natural-gas-fired cogeneration facility - would result in no significant adverse impacts. Finding of No...

  20. EA-1837: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact Performance Verification Laboratory, Morgantown, West Virginia Based on the analyses in the environmental assessment, DOE determined that its...

  1. Rainbows and Leprechauns: Finding Gold in Partnerships (101)

    Office of Energy Efficiency and Renewable Energy (EERE)

    Better Buildings Residential Network Peer Exchange Call Series: Rainbows and Leprechauns: Finding Gold in Partnerships (101), March 17, 2016, call slides and discussion summary.

  2. EA-1539: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Significant Impact EA-1539: Finding of No Significant Impact North Area Right-of-Way Maintenance, California Western Area Power Administration proposes to revise its operation and...

  3. Main Page Main Page: Find energy information and data. Share...

    OpenEI (Open Energy Information) [EERE & EIA]

    Main Page Main Page: Find energy information and data. Share knowledge. Connect with people. energy datasets energy community Main Page energy information open data 0...

  4. Exploring Plasma Science Advances from Fusion Findings to Astrophysica...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Exploring Plasma Science Advances from Fusion Findings to Astrophysical Achievements By ... The latest advances in plasma physics were the focus of more than 1,000 scientists from ...

  5. *** CANCELED *** SCIENCE ON SATURDAY- "Finding a Needle in A...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    MBG Auditorium *** CANCELED *** SCIENCE ON SATURDAY- "Finding a Needle in A (Genomic) Haystack or How Can Computers Help Cure Cancer" Professor Olga G. Troyanskaya Lewis-Sigler ...

  6. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    of the Department of Energy, Waste Isolation Pilot Plant (WIPP) in Eddy County, New Mexico January 13, 2010 EA-1651: Finding of No Significant Impact U-233 Material...

  7. EA-1864: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Impact EA-1864: Finding of No Significant Impact Electrical District 5 - Palo Verde Hub Project The U.S. Department of Energy (DOE) Western Area Power Administration...

  8. Innovative Idaho Site Crews Find Ways to Make Waste Retrieval...

    Energy Saver

    Innovative Idaho Site Crews Find Ways to Make Waste Retrieval Safer, More Efficient Innovative ... program by Idaho Treatment Group, a cleanup contractor. "Retrieval operations ...

  9. EA-1955: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Western Area Power Administration (Western) issued a finding of no significant impact for a proposal to interconnect, via a proposed new substation, a proposed Dakota Plains ...

  10. EA-1974: Finding of No Significant Impact and Mitigation Action...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Bonneville Power Administration issued a finding of no significant impact (FONSI) for the proposed restoration of a tidal marsh in the Columbia River Estuary, near Astoria in ...

  11. Innovative Financing Solutions: Finding Money for Your Energy Efficiency Projects

    Energy.gov [DOE]

    Provides an overview of how to find funding for your energy efficiency projects focusing on l-p agreements. Author: Energy Star

  12. EA-1696: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    EA-1696: Finding of No Significant Impact Pantex Renewable Energy Project, Amarillo, Texas The NNSA has prepared an Environmental Assessment to analyze the potential...

  13. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    and Heating Facilities at the Savannah River Site, South Carolina June 11, 2008 EA-1613: Finding of No Significant Impact Proposed High Explosive Pressing Facility Pantex...

  14. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    of No Significant Impact Proposed Gas Main and Distribution System Upgrade, Pantex Plant, Amarillo, Texas August 26, 2005 EA-1542: Finding of No Significant Impact Burleigh County...

  15. EA-1384: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    The proposed action also involves the installation and operation of the Helios(High Energy Lithography Source) accelerator in the Free Electron Laser (FEL) addition. Finding of No ...

  16. Microsoft Word - Summary of RCA findings and lessons learned...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Work Planning and Scheduling System project Root cause analysis findings and lessons learned Summary BPA stopped the Work Planning and Scheduling System project in February 2012...

  17. Scientist finds new way to predict heat layer troublemaker |...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Scientist finds new way to predict heat layer troublemaker By John Greenwald August 27, ... But heat inevitably flows through the system and becomes separated, or scraped off, from ...

  18. EA-1611: Finding of No Significant Impact | Department of Energy

    Energy.gov [DOE] (indexed site)

    finding of no significant impact (FONSI) for the proposed Colorado Highlands Wind Project.The FONSI describes the information Western used to determine that Western's proposal to...

  19. EA-1921: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact Silver Peak Area Geothermal Exploration Project, Esmeralda County, NV Based on the evaluations in the final EA and subject to the mitigations...

  20. EA-1849: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    EA-1849: Finding of No Significant Impact Ormat Nevada Northern Nevada Geothermal Power Plant Projects: Loan Guarantee for ORMAT LLC's Tuscarora Geothermal Power Plant, Elko ...

  1. EA-1882: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Range Environmental Assessment, DOEEA-1882 (March 2011) More Documents & Publications EA-1822: Final Environmental Assessment EA-1984: Finding of No Significant Impact EA-1555...

  2. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    No Significant Impact Sunflower Wind Project, Hebron, North Dakota September 3, 2014 EA-1984: Finding of No Significant Impact Disposition of Five Signature Properties at Idaho...

  3. EA-1499: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    EA-1499: Finding of No Significant Impact RadiologicalNuclear Countermeasures Test and Evaluation Complex, Nevada Test Site The National Nuclear Security Administration (NNSA) has ...

  4. EA-1628: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Iowa. PDF icon Mitigation Action Plan for the Environmental Assessment, Notice of Wetlands Involvement and Finding of No Significant Impact for Construction and Operation of a...

  5. 10 CFR 850 Notice of Proposed Rulemaking Public Hearing Transcript, Washington, DC – August 11, 2016

    Office of Energy Efficiency and Renewable Energy (EERE)

    Transcript of the Washington, DC public hearing to received comments on the proposed amendments to 10 CFR 850, Chronic Beryllium Disease Prevention Program.

  6. Transcript of Office of Indian Energy Webinar: Inter-Tribal Technical Assistance Energy Providers Network FOA

    Energy.gov [DOE]

    Transcript of the DOE Office of Indian Energy webinar about the Inter-Tribal Technical Assistance Energy Providers Network funding opportunity.

  7. Transcript of April 3, 2013, TAP webinar titled State Energy Strategic Planning

    Energy.gov [DOE]

    U.S. Department of Energy (DOE) Technical Assistance Program (TAP) transcript of a TAP webinar held on April 3, 2013 and dealing with state energy strategic planning.

  8. Successful Partnerships and Lessons from the Field, Part 1, Webinar Transcript

    Energy.gov [DOE]

    Part 1-Successful Partnerships and Lessons from the Field Webinar Transcript, from the U.S. Department of Energy Technical Assistance Program (TAP).

  9. Electricity Advisory Committee Meeting, June 5-6, 2013- Meeting Summaries and Transcripts

    Energy.gov [DOE]

    Meeting summaries and transcripts for the June 5-6, 2013 meeting of the Electricity Advisory Committee.

  10. A new chapter in the transcription SAGA (Journal Article) | SciTech...

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect Search Results Journal Article: A new chapter in the transcription SAGA ... Publication Date: 2012-11-05 OSTI Identifier: 1047303 Resource Type: Journal Article ...

  11. Better Buildings Webinar Transcription- Financial Vehicles within an Integrated Energy Efficiency Program

    Energy.gov [DOE]

    "Financial Vehicles Within an Integrated Energy Efficiency Program," webinar transcript from the U.S. Department of Energy's Better Buildings program.

  12. Transcript of How to Navigate the New and Improved State and Local Solution Center Webinar

    Energy.gov [DOE]

    Transcript of the April 28, 2016 webinar on how to navigate the new and improved State and Local Solution Center website.

  13. Transcript of August 11 Webinar: Dig Deeper into the Recent DOE and HUD Residential PACE

    Office of Energy Efficiency and Renewable Energy (EERE)

    Transcript of the August 11 webinar, Dig Deeper into the Recent DOE and HUD Residential PACE, which covered both FHA and DOE's PACE announcements and their details.

  14. Biodetection System Developed by National Laboratory Researchers Finds Use

    National Nuclear Security Administration (NNSA)

    at Salt Lake City Olympic Games | National Nuclear Security Administration | (NNSA) Biodetection System Developed by National Laboratory Researchers Finds Use at Salt Lake City Olympic Games February 07, 2002 Biodetection System Developed by National Laboratory Researchers Finds Use at Salt Lake City Olympic Games (PDF - 0.04Mb)~

  15. EV Everywhere: Find Electric Vehicle Models | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Find Electric Vehicle Models EV Everywhere: Find Electric Vehicle Models Search Car: Year: -- ALL -- Make: -- ALL -- Market Class: -- ALL -- All-Electric Range: Min -- ALL -- 10 miles 20 miles 30 miles 40 miles 50 miles 60 miles 70 miles 80 miles 90 miles 100 miles 110 miles 120 miles 130+ miles Gasoline Back-Up Available: -- ALL -- No Yes Reset To find out if a plug-in electric vehicle (EV) will work for you, use the menus to the left to sort the available EV models on the market by year, make,

  16. Pivotal roles of p53 transcription-dependent and -independent pathways in manganese-induced mitochondrial dysfunction and neuronal apoptosis

    SciTech Connect

    Wan, Chunhua; Ma, Xa; Shi, Shangshi; Zhao, Jianya; Nie, Xiaoke; Han, Jingling; Xiao, Jing; Wang, Xiaoke; Jiang, Shengyang; Jiang, Junkang

    2014-12-15

    Chronic exposure to excessive manganese (Mn) has been known to lead to neuronal loss and a clinical syndrome resembling idiopathic Parkinson's disease (IPD). p53 plays an integral role in the development of various human diseases, including neurodegenerative disorders. However, the role of p53 in Mn-induced neuronal apoptosis and neurological deficits remains obscure. In the present study, we showed that p53 was critically involved in Mn-induced neuronal apoptosis in rat striatum through both transcription-dependent and -independent mechanisms. Western blot and immunohistochemistrical analyses revealed that p53 was remarkably upregulated in the striatum of rats following Mn exposure. Coincidentally, increased level of cleaved PARP, a hallmark of apoptosis, was observed. Furthermore, using nerve growth factor (NGF)-differentiated PC12 cells as a neuronal cell model, we showed that Mn exposure decreased cell viability and induced apparent apoptosis. Importantly, p53 was progressively upregulated, and accumulated in both the nucleus and the cytoplasm. The cytoplasmic p53 had a remarkable distribution in mitochondria, suggesting an involvement of p53 mitochondrial translocation in Mn-induced neuronal apoptosis. In addition, Mn-induced impairment of mitochondrial membrane potential (ΔΨm) could be partially rescued by pretreatment with inhibitors of p53 transcriptional activity and p53 mitochondrial translocation, Pifithrin-α (PFT-α) and Pifithrin-μ (PFT-μ), respectively. Moreover, blockage of p53 activities with PFT-α and PFT-μ significantly attenuated Mn-induced reactive oxidative stress (ROS) generation and mitochondrial H{sub 2}O{sub 2} production. Finally, we observed that pretreatment with PFT-α and PFT-μ ameliorated Mn-induced apoptosis in PC12 cells. Collectively, these findings implicate that p53 transcription-dependent and -independent pathways may play crucial roles in the regulation of Mn-induced neuronal death. - Highlights: • p53 is robustly

  17. A hairpin within YAP mRNA 3′UTR functions in regulation at post-transcription level

    SciTech Connect

    Gao, Yuen; Wang, Yuan; Feng, Jinyan; Feng, Guoxing; Zheng, Minying; Yang, Zhe; Xiao, Zelin; Lu, Zhanping; Ye, Lihong; Zhang, Xiaodong

    2015-04-03

    The central dogma of gene expression is that DNA is transcribed into messenger RNAs, which in turn serve as the template for protein synthesis. Recently, it has been reported that mRNAs display regulatory roles that rely on their ability to compete for microRNA binding, independent of their protein-coding function. However, the regulatory mechanism of mRNAs remains poorly understood. Here, we report that a hairpin within YAP mRNA 3′untranslated region (3′UTR) functions in regulation at post-transcription level through generating endogenous siRNAs (esiRNAs). Bioinformatics analysis for secondary structure showed that YAP mRNA displayed a hairpin structure (termed standard hairpin, S-hairpin) within its 3′UTR. Surprisingly, we observed that the overexpression of S-hairpin derived from YAP 3′UTR (YAP-sh) increased the luciferase reporter activities of transcriptional factor NF-κB and AP-1 in 293T cells. Moreover, we identified that a fragment from YAP-sh, an esiRNA, was able to target mRNA 3′UTR of NF2 (a member of Hippo-signaling pathway) and YAP mRNA 3′UTR itself in hepatoma cells. Thus, we conclude that the YAP-sh within YAP mRNA 3′UTR may serve as a novel regulatory element, which functions in regulation at post-transcription level. Our finding provides new insights into the mechanism of mRNAs in regulatory function. - Highlights: • An S-hairpin within YAP mRNA 3′UTR possesses regulatory function. • YAP-sh acts as a regulatory element for YAP at post-transcription level. • YAP-sh-3p20, an esiRNA derived from YAP-sh, targets mRNAs of YAP and NF2. • YAP-sh-3p20 depresses the proliferation of HepG2 cells in vitro.

  18. Scientist Finds Nature and Geometry Dancing to the Same Tune...

    Office of Science (SC)

    Scientist Finds Nature and Geometry Dancing to the Same Tune News News Home Featured Articles 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 Science Headlines Science ...

  19. EA-1888: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    of No Significant Impact EA-1888: Finding of No Significant Impact Old Town Fuel and Fiber Proposed Demonstration-Scale Integrated Biorefinery in Old Town, MN DOE has determined...

  20. EA-2002: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    EA-2002: Finding of No Significant Impact Right-of-Way Application for the Tucson-Apache 115-kV Transmission Line, Pima County, Arizona Western Area Power Administration issued a ...

  1. EA-1772: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    The proposed action consists of constructing a road for limited year-round use with the ability for trucks traveling in opposite directions to pass. PDF icon Finding of No ...

  2. EA-1941: Finding of No Significant Impact (FONSI) | Department...

    Energy.gov [DOE] (indexed site)

    Finding of No Significant Impact for proposed improvements to 13 miles of access roads for its existing 115-kV Boyer-Tillamook No. 1 Transmission Line. EA-1941-FONSI-2014.pdf ...

  3. PART THREE REMEDIAL AND CORRECTIVE ACTIONS ARTICLE XIII. FINDINGS...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    a summary of the facts upon which EPA and Ecology are proceeding for purposes of ... This Article contains findings by EPA and Ecology, and shall not be used by any person ...

  4. EA-1948: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    DOE's Western Area Power Administration issued a finding of no significant impact for a proposal to rebuild and upgrade two parallel 4.8-mile transmission lines between the Gila ...

  5. EA-1948: Revised Finding of No Significant Impact | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    On March 21, 2014, DOE's Western Area Power Administration issued a finding of no significant impact (FONSI) for a proposal to rebuild and upgrade two parallel 4.8-mile ...

  6. EA-1976: Finding of No Significant Impact | Department of Energy

    Energy Saver

    EA-1976: Finding of No Significant Impact Emera CNG, LLC Compressed Natural Gas Project, Florida Based upon the EA, DOE has determined that the Emera CNG, LLC Compressed Natural ...

  7. Finding Large Aperture Fractures in Geothermal Resource Areas...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Finding Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component Long-Offset Surface ...

  8. Unique Bioreactor Finds Algae's Sweet Spot - News Feature | NREL

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Unique Bioreactor Finds Algae's Sweet Spot February 18, 2014 Close-up photo of a vial of green algae. Enlarge image Aeration helps algae grow and helps replicate real-life ...

  9. EA-1942: Finding of No Significant Impact | Department of Energy

    Energy Saver

    DOE adopted FERC's EA and issued a Finding of No Significant Impact. PDF icon EA-1942-FONSIl2014.pdf More Documents & Publications Summary of LNG Export Applications of the Lower ...

  10. Finding Six-Figure ROI From Energy Efficiency | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Six-Figure ROI From Energy Efficiency Finding Six-Figure ROI From Energy Efficiency September 28, 2010 - 10:20am Addthis Kevin Craft What are the key facts? Recovery Act funded ...

  11. EA-1995: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    EA-1995: Finding of No Significant Impact Trestle Bay Ecosystem Restoration Project, Clatsop County, Oregon The U.S. Army Corps of Engineers prepared a Feasibility Study and EA ...

  12. Copper is Key in Burning Fat, New Study Finds

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Copper is Key in Burning Fat, New Study Finds A new study led by a Berkeley Lab scientist ... Researchers want to explore if a copper deficiency is linked to obesity and obesity ...

  13. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Winnebago County, Illinois December 2, 2011 EA-1697: Finding of No Significant Impact Right-of-Way Maintenance in the San Joaquin Valley, California November 10, 2011 EA-1862:...

  14. DOE issues Finding of No Significant Impact on Environmental...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    doe logo U.S. Department of Energy Idaho Operations Office Media Contact: Tim Jackson (208) 526-8484 For Immediate Release December 21, 2011 DOE issues Finding of No Significant...

  15. EA-1973: Finding of No Significant Impact and Mitigation Action...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    the project webpage. PDF icon EA-1973-FONSI-MAP-2015 More Documents & Publications EA-1973: Draft Environmental Assessment EA-1901: Finding of No Significant Impact EA-1518: Final

  16. EA-1812: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    DOE has issued a Finding of No Significant Impact (FONSI) based on the information in the Final EA, FONSI, and the Supplement Analysis (SA) prepared to address changes in the ...

  17. Indoor Thermal Factors and Symptoms in Office Workers: Findings...

    Office of Scientific and Technical Information (OSTI)

    from the U.S. EPA BASE Study Citation Details In-Document Search Title: Indoor Thermal Factors and Symptoms in Office Workers: Findings from the U.S. EPA BASE Study You ...

  18. EA-1711: Finding of No Significant Impact | Department of Energy

    Energy.gov [DOE] (indexed site)

    America, Inc to construct and operate a high-volume manufacturing plant to make advanced lithium-ion cells and batteries, would result in no significant adverse impacts. Finding...

  19. EA-1964: FInding of No Significant Impact | Department of Energy

    Energy.gov [DOE] (indexed site)

    13, DOE adopted the NSF EA and issued a finding of no significant impact regarding NEON Project research activities on the Oak Ridge Reservation. EA-1964-FONSI-2013.pdf More...

  20. DOE ZERH Webinar: Tour of Zero, Helping Customers Find Better...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Are you interested in helping homebuyers find a better home at a lower cost? The DOE Tour of ... This national campaign is where you come in. DOE needs Innovation partners vested in ...

  1. Portsmouth Decommissioning and Decontamination Project Director's Final Findings and Order

    Energy.gov [DOE]

    Portsmouth Decommissioning and Decontamination (D&D) Project Director's Final Findings and Order defines the steps for identifying a range of technical alternatives for the D&D and waste...

  2. Sugarcane transgenics expressing MYB transcription factors show improved glucose release

    DOE PAGES [OSTI]

    Poovaiah, Charleson R.; Bewg, William P.; Lan, Wu; Ralph, John; Coleman, Heather D.

    2016-07-15

    In this study, sugarcane, a tropical C4 perennial crop, is capable of producing 30-100 tons or more of biomass per hectare annually. The lignocellulosic residue remaining after sugar extraction is currently underutilized and can provide a significant source of biomass for the production of second-generation bioethanol. As a result, MYB31 and MYB42 were cloned from maize and expressed in sugarcane with and without the UTR sequences. The cloned sequences were 98 and 99 % identical to the published nucleotide sequences. The inclusion of the UTR sequences did not affect any of the parameters tested. There was little difference in plantmore » height and the number of internodes of the MYB-overexpressing sugarcane plants when compared with controls. MYB transgene expression determined by qPCR exhibited continued expression in young and maturing internodes. MYB31 downregulated more genes within the lignin biosynthetic pathway than MYB42. MYB31 and MYB42 expression resulted in decreased lignin content in some lines. All MYB42 plants further analyzed showed significant increases in glucose release by enzymatic hydrolysis in 72 h, whereas only two MYB31 plants released more glucose than control plants. This correlated directly with a significant decrease in acid-insoluble lignin. Soluble sucrose content of the MYB42 transgenic plants did not vary compared to control plants. In conclusion, this study demonstrates the use of MYB transcription factors to improve the production of bioethanol from sugarcane bagasse remaining after sugar extraction.« less

  3. Finding the Right Filling Station for Alternative Vehicles Now Easier

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Finding the Right Filling Station for Alternative Vehicles Now Easier For more information contact: e:mail: Public Affairs Golden, Colo., May 5, 1999 — A new online interactive computer program is taking the guesswork out of finding the fuel needed by the thousands of alternative vehicles on the road today in the United States. The program, called the Alternative Fuel Refueling Station Locator, was developed by the U.S. Department of Energy's (DOE) Alternative Fuels Data Center (AFDC). The AFDC

  4. Energy Department Report Finds Major Potential to Increase Clean

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Hydroelectric Power | Department of Energy Report Finds Major Potential to Increase Clean Hydroelectric Power Energy Department Report Finds Major Potential to Increase Clean Hydroelectric Power April 17, 2012 - 12:39pm Addthis Washington, D.C. -- As part of President Obama's all-out, all-of-the-above energy strategy, the Energy Department today released a renewable energy resource assessment detailing the potential to develop electric power generation at existing dams across the United

  5. EA-1488: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    8: Finding of No Significant Impact EA-1488: Finding of No Significant Impact U-233 Disposition, Medical Isotope Production, and Building 3019 Complex Shutdown at the Oak Ridge National Laboratory, Oak Ridge, Tennessee The U. S. Department of Energy has completed an Environmental Assessment (EA) [DOE/EA-1488] that evaluates the processing of uranium-233 (233U) stored at the Oak Ridge National Laboratory (ORNL) and other small quantities of similar material currently stored at other DOE sites in

  6. EA-1655: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    5: Finding of No Significant Impact EA-1655: Finding of No Significant Impact The Berkeley Lab Laser Accelerator (BELLA) Laser Acquisition, Installation and Use for Research and Development The Proposed Action would create and operate an experimental facility for further advancing the development of laser-driven, plasma-based, particle beam accelerators. An existing, approximately 7,000-square-foot, accelerator laboratory area inside Building 71 at Lawrence Berkeley National Laboratory (LBNL)

  7. EA-1793: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1793: Finding of No Significant Impact Replacement Capability for Disposal of Remote-Handled Low-Level Radioactive Waste Generated at the Department of Energy's Idaho Site DOE prepared an environmental assessment (EA), for the Replacement Capability for the Disposal of Remote-Handled Low-Level Radioactive Waste Generated at the Department of Energy's Idaho Site to evaluate potential environmental impacts related to replacement capability options. Based on the

  8. Find Financing for Energy Efficiency Upgrades | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Commercial Buildings » Find Financing for Energy Efficiency Upgrades Find Financing for Energy Efficiency Upgrades Photo of a block in lower downtown Denver that is part of Living City Block, a DOE Commercial Building Partnerships participant. Financing need not be a barrier to improving energy efficiency in your commercial building. There are federal, state, and local and tax incentives for energy efficiency upgrades, and other financial opportunities to help you meet your energy goals. The

  9. Find Product Categories Covered by Efficiency Programs | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Energy Products & Technologies » Energy-Efficient Products » Find Product Categories Covered by Efficiency Programs Find Product Categories Covered by Efficiency Programs Legend ★ ENERGY STAR △ FEMP Designated O EPEAT ♦ Low Standby Power X WaterSense Federal agencies are required to purchase energy-efficient products. To help buyers meet these requirements, the Federal Energy Management Program (FEMP) maintains acquisition guidance for the following product categories, which are

  10. Finding Large Aperture Fractures in Geothermal Resource Areas Using A

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis | Department of Energy Finding Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Finding Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Fining Large Aperture Fractures in Geothermal Resource Areas Using A

  11. DOE issues Finding of No Significant Impact for the Environmental

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory | Department of Energy issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory DOE issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory February 28, 2014 - 12:00pm

  12. DOE issues Finding of No Significant Impact for the Environmental

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory | Department of Energy DOE issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory DOE issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory February 28, 2014 -

  13. EA-2013: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    3: Finding of No Significant Impact EA-2013: Finding of No Significant Impact Herbicide Application at Three Substations; Imperial County (California), Maricopa and Yuma Counties (Arizona) Western Area Power Administration (Western) issued a FONSI for Western's proposed use of selected herbicides for the treatment of undesirable vegetation within three existing substations on lands administered by the Bureau of Land Management. For more information on this project, see:

  14. EA-2014: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    4: Finding of No Significant Impact EA-2014: Finding of No Significant Impact Y-12 National Security Complex Emergency Operations Center; Oak Ridge, TN Based upon the EA, the National Nuclear Security Administration (NNSA) has determined that the Y-12 Emergency Operations Center project would not be a major Federal action significantly affecting the quality of the human environment within the meaning of NEPA. Therefore, an environmental impact statement is not required. For more information on

  15. EA-2036: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    36: Finding of No Significant Impact EA-2036: Finding of No Significant Impact Sabine Pass Liquefaction Project (design optimization); Cameron Parish, Louisiana In January 2014, the Federal Energy Regulatory Commission (FERC) issued an EA to analyze the potential environmental impacts associated with an application to amend FERC's April 2012 authorization of the Sabine Pass Liquefaction Project in order to optimize its design and operation. In April 2015, Sabine Pass Liquefaction, LLC, and

  16. EA-1321: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1321: Finding of No Significant Impact Proposed Upgrade and Improvement of The National Synchrotron Light Source Complex at Brookhaven National Laboratory, Upton, New York Based on the analysis in the EA, DOE has determined that the continued operation, construction, and upgrades of the National Synchrotron Light Source Complex at Brookhaven National Laboratory do not constitute a major Federal action significantly affecting the quality of the human

  17. EA-1339: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1339: Finding of No Significant Impact Waste Disposition Activities at Paducah Site, Paducah, Kentucky DOE must continue to manage (i.e., treat, store, and dispose) and control its wastes safely, efficiently, and cost effectively in compliance with applicable federal and state laws while protecting Public health and the environment. The wastes considered in the assessment are limited to DOE's ongoing and legacy non-CERCLA waste management operations at the

  18. EA-1523: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1523: Finding of No Significant Impact Modifications at the Strategic Petroleum Reserve's West Hackberry Raw Water Intake Structure Site, Calcasieu Parish, Louisiana The U.S. Department of Energy Strategic Petroleum Reserve (SPR) prepared an Environmental Assessment (EA) (Attachment A) in response to a proposal to modify the West Hackberry (WH) Raw Water Intake Structure (RWIS) located near Hackberry, Louisiana. Based on the results of the EA and

  19. EA-1796: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1796: Finding of No Significant Impact Loan Guarantee to Sempra Generation for Construction of the Mesquite Solar Energy Project; Maricopa County, Arizona Summary Summary DOE's Western Area Power Administration issued a FONSI for the power purchase agreement, on behalf of the Navy, for renewable energy that would be generated by the Mesquite Solar Energy Project. Documents Available For Download EA-1796 FONSI 2015 (1.29 MB) More Documents & Publications

  20. EA-1839: Final Environmental Assessment and Finding of No Significant

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Impact | Department of Energy 39: Final Environmental Assessment and Finding of No Significant Impact EA-1839: Final Environmental Assessment and Finding of No Significant Impact Department of Energy Loan Guarantee to Cogentrix of Alamosa, LLC for Construction of the Cogentrix Solar Project Near Alamosa, Colorado For more information, contact: Ms. Sharon Thomas NEPA Document Manager U.S. Department of Energy 1000 Independence Avenue, SW, LP-10 Washington, DC 20585 Electronic mail:

  1. EA-1891: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    891: Finding of No Significant Impact EA-1891: Finding of No Significant Impact Alvey-Fairview Transmission Line Rebuild Project, Oregon Based on the information in the EA, BPA determined that the proposed action is not a major federal action significantly affecting the quality of the human environment within the meaning of NEPA. Therefore, an EIS will not be prepared and BPA is issuing this FONSI for the proposed action. EA-1891-FONSI-2014.pdf (244.47 KB) More Documents & Publications

  2. Finding new perovskite halides via machine learning (Journal Article) | DOE

    Office of Scientific and Technical Information (OSTI)

    PAGES Finding new perovskite halides via machine learning Title: Finding new perovskite halides via machine learning Advanced materials with improved properties have the potential to fuel future technological advancements. However, identification and discovery of these optimal materials for a specific application is a non-trivial task, because of the vastness of the chemical search space with enormous compositional and configurational degrees of freedom. Materials informatics provides an

  3. EA-1245: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    45: Finding of No Significant Impact EA-1245: Finding of No Significant Impact Kotzebue Wind Installation Project, Kotzebue, Alaska The U.S. Department of Energy's Golden Field Office is considering providing financial assistance to the Kotzebue Electric Association to expand its existing wind installation site near Kotzebue, Alaska. Like many rural Alaskan towns, Kotzebue uses diesel-powered generators to produce electricity, the high cost of which is currently subsidized by the Alaskan State

  4. EA-2000: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-2000: Finding of No Significant Impact Proposed Land Transfer to Develop a General Aviation Airport at the East Tennessee Technology Park Heritage Center, Oak Ridge, Tennessee Oak Ridge Office of Environmental Management issued a FONSI for the proposed title transfer of property located at the East Tennessee Technology Park (ETTP) Heritage Center to the Metropolitan Knoxville Airport Authority for the purpose of constructing and operating a general aviation

  5. EA-2005: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-2005: Finding of No Significant Impact Chromium Plume Control Interim Measure and Plume-Center Characterization, Los Alamos National Laboratory, Los Alamos, NM Based upon the review of the EA prepared by DOE pursuant to the requirements of the National Environmental Policy Act (NEPA), DOE has determined that the planned interim measure to control chromium plume migration while long-term corrective action remedies are evaluated and implemented would not be a

  6. EA-2005: Floodplain Statement of Findings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Floodplain Statement of Findings EA-2005: Floodplain Statement of Findings Chromium Plume Control Interim Measure and Plume-Center Characterization, Los Alamos National Laboratory, Los Alamos, NM The Department of Energy Environmental Management Los Alamos Field Office issued a Final Environmental Assessment for the Chromium Plume Control Interim Measure and Plume-Center Characterization at the Los Alamos National Laboratory, Los Alamos, New Mexico (DOE/EA-2005). A Floodplain Assessment was

  7. EA-1985: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-1985: Finding of No Significant Impact Virginia Offshore Wind Technology Advancement Project on the Atlantic Outer Continental Shelf Offshore Virginia DOE is proposing to fund Virginia Electric and Power Company's Virginia Offshore Wind Technology Advancement Project (VOWTAP). The proposed VOWTAP project consists of design, construction and operation of a 12 megawatt offshore wind facility located approximately 24 nautical miles off the coast of Virginia

  8. EA-2001: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-2001: Finding of No Significant Impact Energy Efficiency Standards for New Federal Commercial and Multi-Family High-Rise Residential Buildings' Baseline Standards Update (RIN 1904-AD39) Based upon the EA, DOE has determined that revising the Federal building energy efficiency standards for commercial and multi-family high-rise residential buildings to ASHRAE 90.1-2013 would not be a major Federal action significantly affecting the quality of the human

  9. EA-2004: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-2004: Finding of No Significant Impact The Seneca Nation Wind Turbine Project, Cattaraugus Territory, Erie County, New York Based upon the EA, DOE has determined that authorizing the expenditure of federal funding to the Seneca Nation of Indians to design, permit, and construct up to a 2.0 megawatt wind turbine on Tribal common lands in the Cattaraugus Territory, New York would not be a major Federal action significantly affecting the quality of the human

  10. EA-2017: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finding of No Significant Impact EA-2017: Finding of No Significant Impact Braddock Locks and Dam Hydro Electric Project Based upon the review of the EA prepared by the Federal Energy Regulatory Commission (FERC) and adopted by DOE pursuant to the requirements of the National Environmental Policy Act (NEPA), DOE has determined that authorizing the expenditure of federal funding to Hydro Green Energy, LLC to fabricate, install, and operate one interchangeable Modular Bulb Turbine (MBT), which

  11. NERSC Resources Help Find Roots of MJO Modeling Mismatches

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    NERSC Resources Help Find Roots of MJO Modeling Mismatches NERSC Resources Help Find Roots of MJO Modeling Mismatches April 18, 2016 MJOmodeling From Calcutta, India, to Seattle, Wash. and beyond, the MJO can bring storms and plenty of rain in wet years. PNNL researchers are using field data and NERSC supercomputers to better model how the MJO operates and understand its interaction regional weather systems around the world. Image: PNNL An Alaska-sized pulse of clouds and precipitation

  12. Neutrons find "missing" magnetism of plutonium

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Neutrons find "missing" magnetism of plutonium Neutrons find "missing" magnetism of plutonium Groundbreaking work at two Department of Energy national laboratories has confirmed plutonium's magnetism, which scientists have long theorized but have never been able to experimentally observe. July 10, 2015 Doug Abernathy, left, ARCS instrument scientist at Oak Ridge National Laboratory, and Marc Janoschek, Los Alamos National Laboratory, prepare their sample for experiments at

  13. Finding new perovskite halides via machine learning (Journal Article) |

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect Finding new perovskite halides via machine learning Citation Details In-Document Search Title: Finding new perovskite halides via machine learning Advanced materials with improved properties have the potential to fuel future technological advancements. However, identification and discovery of these optimal materials for a specific application is a non-trivial task, because of the vastness of the chemical search space with enormous compositional and configurational degrees of

  14. From pandemic preparedness to biofuel production: Tobacco finds its

    Office of Scientific and Technical Information (OSTI)

    biotechnology niche in North America (Journal Article) | DOE PAGES From pandemic preparedness to biofuel production: Tobacco finds its biotechnology niche in North America Title: From pandemic preparedness to biofuel production: Tobacco finds its biotechnology niche in North America As part of my NSD Innovation awarded funds (95470 Powell Innovation: charge code N38540) one my deliverables was a review article for journal submission summarizing my work on this project. My NSD Innovation

  15. Study finds surprising variability in shape of Van Allen Belts

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Study finds surprising variability in shape of Van Allen Belts Study finds surprising variability in shape of Van Allen Belts Understanding the shape and size of the belts, which shrink and swell in response to magnetic storms coming from the sun, is crucial for protecting our technology in space. February 23, 2016 1. The traditional idea of the radiation belts includes a larger, more dynamic outer belt and a smaller, more stable inner belt with an empty slot region separating the two. However,

  16. Two Independent Assessments Find the Department of Energy's Yucca Mountain

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Project is on Track | Department of Energy Independent Assessments Find the Department of Energy's Yucca Mountain Project is on Track Two Independent Assessments Find the Department of Energy's Yucca Mountain Project is on Track December 13, 2007 - 4:44pm Addthis WASHINGTON, DC - The U.S. Department of Energy (DOE) Director of the Office of Civilian Radioactive Waste Management (OCRWM) today released two independent assessments addressing areas critical to the overall success of the Yucca

  17. Find EERE Funding Opportunity Announcements | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Find EERE Funding Opportunity Announcements Find EERE Funding Opportunity Announcements Most EERE awards are selected competitively through funding opportunity announcements (FOAs) and solicitations. Competition ensures that EERE selects awards based on merit, which results in better award outcomes. All FOAs, both open and closed, are posted on two primary websites. Graphic of two circles that connect with grants.gov and EERE Exchange text. Grants.gov Grants.gov is a comprehensive website that

  18. Curiosity rover finds evidence of Mars' primitive continental crust

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Curiosity rover finds evidence of Mars' primitive continental crust Curiosity rover finds evidence of Mars' primitive continental crust This is the first discovery of a potential "continental crust" on Mars. July 13, 2015 Igneous clast named Harrison embedded in a conglomerate rock in Gale crater, Mars, shows elongated light-toned feldspar crystals. The mosaic merges an image from Mastcam with higher-resolution images from ChemCam's Remote Micro-Imager. Credit:

  19. CREED: Helping Colorado Cleantech Startups Find the Money - News Releases |

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    NREL CREED: Helping Colorado Cleantech Startups Find the Money January 19, 2012 The Colorado Center for Renewable Energy Economic Development (CREED) at the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) invites cleantech entrepreneurs to attend the next event in its Entrepreneur Series. The Jan. 26 class, "Financing Your Cleantech Startup," will provide cleantech entrepreneurs with an overview of how to find capital for a startup. "If you are in

  20. Extracellular Matrix-Regulated Gene Expression RequiresCooperation of SWI/SNF and Transcription Factors

    SciTech Connect

    Xu, Ren; Spencer, Virginia A.; Bissell, Mina J.

    2006-05-25

    Extracellular cues play crucial roles in the transcriptional regulation of tissue-specific genes, but whether and how these signals lead to chromatin remodeling is not understood and subject to debate. Using chromatin immunoprecipitation (ChIP) assays and mammary-specific genes as models, we show here that extracellular matrix (ECM) molecules and prolactin cooperate to induce histone acetylation and binding of transcription factors and the SWI/SNF complex to the {beta}- and ?-casein promoters. Introduction of a dominant negative Brg1, an ATPase subunit of SWI/SNF complex, significantly reduced both {beta}- and ?-casein expression, suggesting that SWI/SNF-dependent chromatin remodeling is required for transcription of mammary-specific genes. ChIP analyses demonstrated that the ATPase activity of SWI/SNF is necessary for recruitment of RNA transcriptional machinery, but not for binding of transcription factors or for histone acetylation. Coimmunoprecipitation analyses showed that the SWI/SNF complex is associated with STAT5, C/EBP{beta}, and glucocorticoid receptor (GR). Thus, ECM- and prolactin-regulated transcription of the mammary-specific casein genes requires the concerted action of chromatin remodeling enzymes and transcription factors.

  1. Curved track segment finding using Tiny Triplet Finder (TTF)

    SciTech Connect

    Wu, Jin-Yuan; Wang, M.; Gottschalk, E.; Shi, Z.; /Fermilab

    2006-11-01

    We describe the applications of a track segment recognition scheme called the Tiny Triplet Finder (TTF) that involves the grouping of three hits satisfying a constraint forming of a track segment. The TTF was originally developed solving straight track segment finding problem, however, it is also suitable in many curved track segment finding problems. The examples discussed in this document are among popular detector layouts in high-energy/nuclear physics experiments. Although it is not practical to find a universal recipe for arbitrary detector layouts, the method of the TTF application is illustrated via the discussion of the examples. Generally speaking, whenever the data item to be found in a pattern recognition problem contains two free parameters, and if the constraint connecting the measurements and the two free parameters has an approximate shift invariant property, the Tiny Triplet Finder can be used.

  2. Transcript of Tribal Energy and Economic Development July Webinar: Project Development for Long-Term Tribal Energy

    Energy.gov [DOE]

    Transcript of Tribal Energy and Economic Development July Webinar: Project Development for Long-Term Tribal Energy.

  3. Curiosity rover finds weird 'egg rock' meteorite on Mars

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Curiosity rover finds weird 'egg rock' meteorite on Mars Curiosity rover finds weird 'egg rock' meteorite on Mars NASA's Mars rover Curiosity stumbled upon a dark grey, golf-ball-size object last week that looks nothing like the typical red-orange rocks that are normally seen on Mars. November 7, 2016 Copperhead Snake on Dead Leaves, watercolor by Abbott Handerson Thayer (1849-1921) This dark, lumpy, golf ball-size object is an iron-nickel meteorite. NASA's Curiosity rover discovered it on Mars

  4. Finding & Sharing Information about Energy Efficiency | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Energy Finding & Sharing Information about Energy Efficiency Finding & Sharing Information about Energy Efficiency June 29, 2011 - 12:27pm Addthis Drew Bittner Writer/Editor, Office of Energy Efficiency and Renewable Energy Vacation time should be relaxing, but it's often just a different kind of work. Doesn't mean it isn't fun but I seem to come home from vacation more tired than when I left. This past week was spent on the Gulf Coast, where my mother turned 81. One of my goals was

  5. NREL Study Finds Barotrauma Not Guilty | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Study Finds Barotrauma Not Guilty NREL Study Finds Barotrauma Not Guilty January 10, 2013 - 3:18pm Addthis This is an excerpt from the Fourth Quarter 2012 edition of the Wind Program R&D Newsletter. For years scientists have hypothesized that the increasing number of bat fatalities in wind farms is due to barotrauma-related injuries. But according to a study presented by the National Renewable Energy Laboratory (NREL) at the ninth biennial Wind Wildlife Research Meeting in Denver, Colorado,

  6. Finding the needle in the haystack: Algorithms for conformational optimization

    SciTech Connect

    Andricioaei, I.; Straub, J.E.

    1996-09-01

    Algorithms are given for comformational optimization of proteins. The protein folding problems is regarded as a problem of global energy mimimization. Since proteins have hundreds of atoms, finding the lowest-energy conformation in a many-dimensional configuration space becomes a computationally demanding problem.{copyright} {ital American Institute of Physics.}

  7. Transcript of March 28, 2013, TAP webinar titled Internal Benchmarking Outreach and Data Collection Techniques

    Office of Energy Efficiency and Renewable Energy (EERE)

    U.S. Department of Energy (DOE) Technical Assistance Program (TAP) transcript of a TAP webinar held on March 28, 2013 and dealing with internal benchmarking outreach and data collection techniques.

  8. Transcript of April 11, 2013, TAP webinar titled Financing Energy Upgrades for K-12 School Districts

    Energy.gov [DOE]

    U.S. Department of Energy (DOE) Technical Assistance Program (TAP) transcript of a TAP webinar held on April 11, 2013 and dealing with how to finance energy efficiency upgrades for K-12 school districts.

  9. Impact of the N-Terminal Domain of STAT3 in STAT3-Dependent Transcript...

    Office of Scientific and Technical Information (OSTI)

    Impact of the N-Terminal Domain of STAT3 in STAT3-Dependent Transcriptional Activity Citation Details In-Document Search Title: Impact of the N-Terminal Domain of STAT3 in ...

  10. Transcriptional program for nitrogen starvation-induced lipid accumulation in Chlamydomonas reinhardtii

    DOE PAGES [OSTI]

    Garcia de Lomana, Adrian Lopez; Schäuble, Sascha; Valenzuela, Jacob; Imam, Saheed; Carter, Warren; Bilgin, Damla D.; Yohn, Christopher B.; Turkarslan, Serdar; Reiss, David J.; Orellana, Monica V.; et al

    2015-12-02

    Algae accumulate lipids to endure different kinds of environmental stresses including macronutrient starvation. Although this response has been extensively studied, an in depth understanding of the transcriptional regulatory network (TRN) that controls the transition into lipid accumulation remains elusive. In this study, we used a systems biology approach to elucidate the transcriptional program that coordinates the nitrogen starvation-induced metabolic readjustments that drive lipid accumulation in Chlamydomonas reinhardtii. We demonstrate that nitrogen starvation triggered differential regulation of 2147 transcripts, which were co-regulated in 215 distinct modules and temporally ordered as 31 transcriptional waves. An early-stage response was triggered within 12 minmore » that initiated growth arrest through activation of key signaling pathways, while simultaneously preparing the intracellular environment for later stages by modulating transport processes and ubiquitin-mediated protein degradation. Subsequently, central metabolism and carbon fixation were remodeled to trigger the accumulation of triacylglycerols. Further analysis revealed that these waves of genome-wide transcriptional events were coordinated by a regulatory program orchestrated by at least 17 transcriptional regulators, many of which had not been previously implicated in this process. We demonstrate that the TRN coordinates transcriptional downregulation of 57 metabolic enzymes across a period of nearly 4 h to drive an increase in lipid content per unit biomass. Notably, this TRN appears to also drive lipid accumulation during sulfur starvation, while phosphorus starvation induces a different regulatory program. The TRN model described here is available as a community-wide web-resource at http://networks.systemsbiology.net/chlamy-portal. In conclusion, in this work, we have uncovered a comprehensive mechanistic model of the TRN controlling the transition from N starvation to lipid

  11. Transcriptional program for nitrogen starvation-induced lipid accumulation in Chlamydomonas reinhardtii

    SciTech Connect

    Garcia de Lomana, Adrian Lopez; Schäuble, Sascha; Valenzuela, Jacob; Imam, Saheed; Carter, Warren; Bilgin, Damla D.; Yohn, Christopher B.; Turkarslan, Serdar; Reiss, David J.; Orellana, Monica V.; Price, Nathan D.; Baliga, Nitin S.

    2015-12-02

    Algae accumulate lipids to endure different kinds of environmental stresses including macronutrient starvation. Although this response has been extensively studied, an in depth understanding of the transcriptional regulatory network (TRN) that controls the transition into lipid accumulation remains elusive. In this study, we used a systems biology approach to elucidate the transcriptional program that coordinates the nitrogen starvation-induced metabolic readjustments that drive lipid accumulation in Chlamydomonas reinhardtii. We demonstrate that nitrogen starvation triggered differential regulation of 2147 transcripts, which were co-regulated in 215 distinct modules and temporally ordered as 31 transcriptional waves. An early-stage response was triggered within 12 min that initiated growth arrest through activation of key signaling pathways, while simultaneously preparing the intracellular environment for later stages by modulating transport processes and ubiquitin-mediated protein degradation. Subsequently, central metabolism and carbon fixation were remodeled to trigger the accumulation of triacylglycerols. Further analysis revealed that these waves of genome-wide transcriptional events were coordinated by a regulatory program orchestrated by at least 17 transcriptional regulators, many of which had not been previously implicated in this process. We demonstrate that the TRN coordinates transcriptional downregulation of 57 metabolic enzymes across a period of nearly 4 h to drive an increase in lipid content per unit biomass. Notably, this TRN appears to also drive lipid accumulation during sulfur starvation, while phosphorus starvation induces a different regulatory program. The TRN model described here is available as a community-wide web-resource at http://networks.systemsbiology.net/chlamy-portal. In conclusion, in this work, we have uncovered a comprehensive mechanistic model of the TRN controlling the transition from N starvation to lipid accumulation

  12. Comparative Analysis of Transcription Factors Families across Fungal Tree of Life

    SciTech Connect

    Salamov, Asaf; Grigoriev, Igor

    2015-03-19

    Transcription factors (TFs) are proteins that regulate the transcription of genes, by binding to specific DNA sequences. Based on literature (Shelest, 2008; Weirauch and Hughes,2011) collected and manually curated list of DBD Pfam domains (in total 62 DBD domains) We looked for distribution of TFs in 395 fungal genomes plus additionally in plant genomes (Phytozome), prokaryotes(IMG), some animals/metazoans and protists genomes

  13. Finding Chemical Reaction Paths with a Multilevel Preconditioning Protocol

    SciTech Connect

    Kale, Seyit; Sode, Olaseni; Weare, Jonathan; Dinner, Aaron R.

    2014-11-07

    Finding transition paths for chemical reactions can be computationally costly owing to the level of quantum-chemical theory needed for accuracy. Here, we show that a multilevel preconditioning scheme that was recently introduced (Tempkin et al. J. Chem. Phys. 2014, 140, 184114) can be used to accelerate quantum-chemical string calculations. We demonstrate the method by finding minimum-energy paths for two well-characterized reactions: tautomerization of malonaldehyde and Claissen rearrangement of chorismate to prephanate. For these reactions, we show that preconditioning density functional theory (DFT) with a semiempirical method reduces the computational cost for reaching a converged path that is an optimum under DFT by several fold. In conclusion, the approach also shows promise for free energy calculations when thermal noise can be controlled.

  14. Finding Chemical Reaction Paths with a Multilevel Preconditioning Protocol

    DOE PAGES [OSTI]

    Kale, Seyit; Sode, Olaseni; Weare, Jonathan; Dinner, Aaron R.

    2014-11-07

    Finding transition paths for chemical reactions can be computationally costly owing to the level of quantum-chemical theory needed for accuracy. Here, we show that a multilevel preconditioning scheme that was recently introduced (Tempkin et al. J. Chem. Phys. 2014, 140, 184114) can be used to accelerate quantum-chemical string calculations. We demonstrate the method by finding minimum-energy paths for two well-characterized reactions: tautomerization of malonaldehyde and Claissen rearrangement of chorismate to prephanate. For these reactions, we show that preconditioning density functional theory (DFT) with a semiempirical method reduces the computational cost for reaching a converged path that is an optimum undermore » DFT by several fold. In conclusion, the approach also shows promise for free energy calculations when thermal noise can be controlled.« less

  15. Summer interns present research findings in poster session | Princeton

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Plasma Physics Lab Summer interns present research findings in poster session By Raphael Rosen August 31, 2015 Tweet Widget Google Plus One Share on Facebook New Jersey Assemblywoman Donna Simon Talks With SULI Intern Cara Bagley (Photo by Elle Starkman) New Jersey Assemblywoman Donna Simon Talks With SULI Intern Cara Bagley Gallery: Physicist Charles Skinner and Intern Amanda Lewis (Photo by Elle Starkman) Physicist Charles Skinner and Intern Amanda Lewis Interns Matthew Lotocki, Zack

  16. NREL Research into Perovskites Yields New Findings | PV | NREL

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Research into Perovskites Yields New Findings March 28, 2016 Researchers at the National Renewable Energy Laboratory have been examining ways to increase the efficiency and stability of solar cells based on hybrid organic-inorganic perovskite absorbers. Two recently published papers show the results of photoemission spectroscopy and time-resolved optical spectroscopy, which quantify the energetics and carrier dynamics that occur at interfaces between the perovskite absorber and transport layers.

  17. NREL Scientists Find Key Function for Ferredoxins in Algae Hydrogen

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Production - News Releases | NREL Scientists Find Key Function for Ferredoxins in Algae Hydrogen Production Two of six iron-rich proteins shown to have role in algae metabolism; discovery could lead to enhanced hydrogen production February 10, 2014 Scientists at the Energy Department's National Renewable Energy Laboratory have demonstrated that just two of six iron-sulfur-containing ferredoxins in a representative species of algae promote electron transfers to and from hydrogenases. The

  18. Livermore research finds that climate models overestimate rainfall

    National Nuclear Security Administration (NNSA)

    increases | National Nuclear Security Administration | (NNSA) Livermore research finds that climate models overestimate rainfall increases Monday, December 21, 2015 - 12:00am Researchers at Lawrence Livermore National Laboratory and collaborators have found that most climate models overestimate the increase in global precipitation due to climate change. Specifically, the team looked at 25 models and found they underestimate the increase in absorption of sunlight by water vapor as the

  19. DOE issues Finding of No Significant Impact for the Environmental

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory Tim Jackson, (208) 526-8484 For Immediate Release: February 28, 2014 DOE issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory The U.S. Department of Energy (DOE) has determined that resuming transient testing of nuclear fuels and materials at Idaho National Laboratory (INL)

  20. ADOPTION OF ENVIRONMENTAL ASSESSMENT and FINDING OF NO SIGNIFICANT IMPACT;

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    ADOPTION OF ENVIRONMENTAL ASSESSMENT and FINDING OF NO SIGNIFICANT IMPACT; DETERMINATION OF NO SIGNIFICANT IMPACT DOE/EA-1868: MANHATTAN PROJECT SITES Los Alamos, New Mexico; Hanford, Washington; Oak Ridge, Tennessee; Dayton, Ohio BACKGROUND: 'The National Park Service (NPS) issued an Environmental Assessment (September 2010) in consultation with DOE for a proposed action of recommending that Congress authorize an innovative Manhattan Project National Historical Park. The Park Service selected

  1. Researchers find potential key for unlocking biomass energy

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Unlocking biomass energy Researchers find potential key for unlocking biomass energy Potential pretreatment method that can make plant cellulose five times more digestible by enzymes that convert it into ethanol, a useful biofuel. July 20, 2011 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy sources, to plasma physics and new

  2. Finding Large Aperture Fractures in Geothermal Resource Areas Using a

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Three-Component Long-Offset Surface Seismic Survey | Department of Energy a Three-Component Long-Offset Surface Seismic Survey Finding Large Aperture Fractures in Geothermal Resource Areas Using a Three-Component Long-Offset Surface Seismic Survey DOE Geothermal Peer Review 2010 - Presentation. Project summary: Drilling into large aperture open fractures (LAFs) typically yield production wells with high productivity and low pressure drawdown. Developing geophysical and geologic techniques

  3. Finding and Winning Simplified Acquisitions to Grow Your Business

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Find Simplified Acquisitions... What They Buy. How Much They Spend. Which 'slices' of the Simplified pie can your company pursue? More than half of all Simplified Acquisition obligations went to small business concerns, set-aside or not! FSS (Federal Supply Schedule) *Multiple Award, Open Solicitation A simplified process of acquiring commonly used supplies and services in varying quantities at lower prices while obtaining discounts associated with volume buying. GSA and VA are the only

  4. EA-1651: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    | Department of Energy 8: Solyndra, Inc. Photovoltaic Manufacturing Facility in Fremont, CA EA-1638: Solyndra, Inc. Photovoltaic Manufacturing Facility in Fremont, CA March 2, 2009 EA-1638: Final Environmental Assessment Loan Guarantee to Solyndra, Inc. for Construction of A Photovoltaic Manufacturing Facility and Leasing of an Existing Commercial Facility in Fremont, California March 31, 2009 EA-1638: Finding of No Significant Impact Loan Guarantee to Solyndra, Inc. for Construction of a

  5. EA-1815: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    5: Diamond Green Diesel Facility in Norco, LA EA-1795: Diamond Green Diesel Facility in Norco, LA April 1, 2011 EA-1795: Final Environmental Assessment Loan Guarantee to Diamond Green Diesel, LLC for Construction of the Diamond Green Diesel Facility in Norco, Louisiana April 18, 2011 EA-1795: Finding of No Significant Impact Loan Guarantee to Diamond Green Diesel for Construction of the Diamond Green Diesel Facility in Norco, Louisiana

    Final Environmental Assessment EA-1795: Final

  6. EA-1929: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    CA | Department of Energy 0: California Valley Solar Ranch Project in San Luis Obispo County, CA EA-1840: California Valley Solar Ranch Project in San Luis Obispo County, CA August 3, 2011 EA-1840: Final Environmental Assessment California Valley Solar Ranch Project in San Luis Obispo and Kern Counties, California August 3, 2011 EA-1840: Finding of No Significant Impact Loan Guarantee to High Plains II, LLC for the California Valley Solar Ranch Project in San Luis Obispo County and Kern

  7. FOR THE SUPPLEMENTAL ENVIRONMENTAL ASSESSMENT/FINDING OF NO

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    2 FOR THE SUPPLEMENTAL ENVIRONMENTAL ASSESSMENT/FINDING OF NO SIGNIFICANT IMPACT FOR THE UNIVERSITY OF MAINE'S DEEPWATER OFFSHORE FLOATING WIND TURBINE TESTING AND DEMONSTRATION PROJECT CASTINE, MAINE DOE/EA-1792-S1 US Department of Energy Office of Energy Efficiency and Renewable Energy Golden, Colorado November 2014 Supplement Analysis - 2 i November 20, 2014 DOE/EA-1792-S1 CONTENTS 1.0 Background

  8. Finding Solutions to Solar's Soft Cost Dilemma | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Solutions to Solar's Soft Cost Dilemma Finding Solutions to Solar's Soft Cost Dilemma January 8, 2013 - 1:22pm Addthis Brian Webster installs rooftop solar panels on a home in Englewood, Colorado. The Energy Department is working to streamline rooftop solar installations so that its faster, easier and cheaper for Americans to go solar. | Photo courtesy of Dennis Schroeder, NREL. Brian Webster installs rooftop solar panels on a home in Englewood, Colorado. The Energy Department is working to

  9. NREL analysis finds tax credit extensions can impact renewable energy

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    deployment and electric sector CO2 emissions - News Releases | NREL analysis finds tax credit extensions can impact renewable energy deployment and electric sector CO2 emissions February 22, 2016 The Energy Department's National Renewable Energy Laboratory (NREL) today released new analysis exploring the potential impact of recently extended federal tax credits on the deployment of renewable generation technologies and related U.S. electric sector carbon dioxide (CO2) emissions. The report,

  10. Get connected: University research departments and libraries can find tools

    Office of Scientific and Technical Information (OSTI)

    and resources from DOE at .EDUconnections | OSTI, US Dept of Energy Office of Scientific and Technical Information connected: University research departments and libraries can find tools and resources from DOE at .EDUconnections Back to the OSTI News Listing for 2009 University research departments and libraries can now easily connect to important resources from the U.S. Department of Energy. Check out the specialized tools and services; visit the spotlight page spotlight to see examples of

  11. SUPPLEMENT ANALYSIS FOR THE SUPPLEMENTAL ENVIRONMENTAL ASSESSMENT/FINDING OF

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    FOR THE SUPPLEMENTAL ENVIRONMENTAL ASSESSMENT/FINDING OF NO SIGNIFICANT IMPACT FOR THE UNIVERSITY OF MAINE'S DEEPWATER OFFSHORE FLOATING WIND TURBINE TESTING AND DEMONSTRATION PROJECT CASTINE, MAINE DOE/EA-1792-S1 US Department of Energy Office of Energy Efficiency and Renewable Energy Golden, Colorado September 2013 Supplement Analysis i September 5, 2013 DOE/EA-1792-S1 CONTENTS 1.0 Background

  12. Finding a suitable platinum substitute for fuel cells | Argonne National

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Laboratory Finding a suitable platinum substitute for fuel cells By Jared Sagoff * April 2, 2014 Tweet EmailPrint Researchers working on the next generation of fuel cells have been facing a platinum conundrum - and it's one they're fighting hard to solve. Platinum, one of the most costly precious metals, works exceptionally well as the catalyst inside of a fuel cell. At the anode, platinum helps to strip the electron from hydrogen to form single protons, while at the cathode, platinum

  13. Finding Large Aperture Fractures in Geothermal Resource Areas Using A

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis | Department of Energy A Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Finding Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Fining Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic

  14. Scientist finds new way to predict heat layer troublemaker | Princeton

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Plasma Physics Lab Scientist finds new way to predict heat layer troublemaker By John Greenwald August 27, 2012 Tweet Widget Google Plus One Share on Facebook Researchers at a recent worldwide conference on fusion power have confirmed the surprising accuracy of a new model for predicting the size of a key barrier to fusion that a top scientist at the U.S. Department of Energy's Princeton Plasma Physics Laboratory (PPPL) has developed. The model could serve as a starting point for overcoming

  15. Finding renewable energy materials using one screensaver at a time:

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Long-Term Solutions for Nuclear Waste Finding Long-Term Solutions for Nuclear Waste December 21, 2015 - 1:00pm Addthis The Department of Energy is working toward long-term solutions for nuclear waste storage. | Photo by <a href="https://www.flickr.com/photos/mandj98/">James Marvin Phelps</a>. The Department of Energy is working toward long-term solutions for nuclear waste storage. | Photo by James Marvin Phelps. Franklin (Lynn) Orr Franklin (Lynn) Orr Under Secretary for

  16. Microsoft Word - fact sheet alternatives and key findings 090214.docx

    National Nuclear Security Administration (NNSA)

    Alternatives and Key Findings of the Draft SEIS ALTERNATIVES The Draft SEIS evaluates a range of reactor site and tritium production capacity alternatives. Seven alternatives are analyzed in the Draft SEIS:  Alternative 1 Watts Bar site only (2,500 TPBARs maximum). This is the preferred alternative.  Alternative 2 Sequoyah site only (2,500 TPBARs maximum).  Alternative 3 Both Watts Bar and Sequoyah sites (2,500 TPBARs maximum).  Alternative 4 Watts Bar site only (5,000 TPBARs

  17. Exploring Plasma Science Advances from Fusion Findings to Astrophysical

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Achievements | Princeton Plasma Physics Lab Exploring Plasma Science Advances from Fusion Findings to Astrophysical Achievements By John Greenwald December 4, 2012 Tweet Widget Google Plus One Share on Facebook The latest advances in plasma physics were the focus of more than 1,000 scientists from around the world who gathered in Providence, R.I., from Oct. 29 through Nov. 2 for the 54th Annual Meeting of the American Physical Society's Division of Plasma Physics (APS-DPP). Papers, posters

  18. Graph Coarsening for Path Finding in Cybersecurity Graphs

    SciTech Connect

    Hogan, Emilie A.; Johnson, John R.; Halappanavar, Mahantesh

    2013-01-01

    n the pass-the-hash attack, hackers repeatedly steal password hashes and move through a computer network with the goal of reaching a computer with high level administrative privileges. In this paper we apply graph coarsening in network graphs for the purpose of detecting hackers using this attack or assessing the risk level of the network's current state. We repeatedly take graph minors, which preserve the existence of paths in the graph, and take powers of the adjacency matrix to count the paths. This allows us to detect the existence of paths as well as find paths that have high risk of being used by adversaries.

  19. Transportation Energy Futures: Project Overview and Findings (Presentation)

    SciTech Connect

    Not Available

    2013-03-01

    The U.S. Department of Energy-sponsored Transportation Energy Futures (TEF) project examines how combining multiple strategies could reduce both GHG emissions and petroleum use by 80%. The project's primary objective was to help inform domestic decisions about transportation energy strategies, priorities, and investments, with an emphasis on previously underexplored opportunities related to energy efficiency and renewable energy in light-duty vehicles, non-light-duty vehicles, fuels, and transportation demand. This PowerPoint provides an overview of the project and its findings.

  20. (Findings of the Costa Rica power sector efficiency study)

    SciTech Connect

    Waddle, D.B.

    1990-10-08

    To present findings of the Costa Rica Power Sector Efficiency Study to the Instituto Costarricense de Electridad, and to the Ministry of Energy, Natural Resources and Mining. To discuss the progress and plans for the Central American Rural Electrification Project with US Agency for International Development (USAID)/Regional Office Central American Program (ROCAP). I traveled to San Jose, Costa Rica to present the findings of the Costa Rica Power Sector Efficiency Study to our counterparts in the utility and the Ministry of Energy. Discussions were held with line level managers at Instituto Costarricensede Electricidad (ICE) and Ministry of Energy Mines and Natural Resources (MIRENEM), as well as a plan of action set for the final stage of the project. Discussions were held for a one day period with both the bilateral Agency for International Development (AID) and the regional AID mission regarding the need for a similar study in Guatemala and matters directly pertaining to the Central American Rural Electrification Study (CARES) project.

  1. Finding Text Information in the Ocean of Electronic Documents

    SciTech Connect

    Medvick, Patricia A.; Calapristi, Augustin J.

    2003-02-05

    Information management in natural resources has become an overwhelming task. A massive amount of electronic documents and data is now available for creating informed decisions. The problem is finding the relevant information to support the decision-making process. Determining gaps in knowledge in order to propose new studies or to determine which proposals to fund for maximum potential is a time-consuming and difficult task. Additionally, available data stores are increasing in complexity; they now may include not only text and numerical data, but also images, sounds, and video recordings. Information visualization specialists at Pacific Northwest National Laboratory (PNNL) have software tools for exploring electronic data stores and for discovering and exploiting relationships within data sets. These provide capabilities for unstructured text explorations, the use of data signatures (a compact format for the essence of a set of scientific data) for visualization (Wong et al 2000), visualizations for multiple query results (Havre et al. 2001), and others (http://www.pnl.gov/infoviz ). We will focus on IN-SPIRE, a MS Windows vision of PNNL’s SPIRE (Spatial Paradigm for Information Retrieval and Exploration). IN-SPIRE was developed to assist information analysts find and discover information in huge masses of text documents.

  2. Utility of Social Modeling for Proliferation Assessment - Preliminary Findings

    SciTech Connect

    Coles, Garill A.; Gastelum, Zoe N.; Brothers, Alan J.; Thompson, Sandra E.

    2009-07-16

    Often the methodologies for assessing proliferation risk are focused around the inherent vulnerability of nuclear energy systems and associated safeguards. For example an accepted approach involves ways to measure the intrinsic and extrinsic barriers to potential proliferation. This paper describes preliminary investigation into non-traditional use of social and cultural information to improve proliferation assessment and advance the approach to assessing nuclear material diversion. Proliferation resistance assessment, safeguard assessments and related studies typically create technical information about the vulnerability of a nuclear energy system to diversion of nuclear material. The purpose of this research project is to find ways to integrate social information with technical information by explicitly considering the role of culture, groups and/or individuals to factors that impact the possibility of proliferation. When final, this work is expected to describe and demonstrate the utility of social science modeling in proliferation and proliferation risk assessments.

  3. Parallel halo finding in N-body cosmology simulations

    SciTech Connect

    Pfitzner, D.W.; Salmon, J.K.

    1996-12-31

    Cosmological N-body simulations on parallel computers produce large datasets - about five hundred Megabytes at a single output time, or tens of Gigabytes over the course of a simulation. These large datasets require further analysis before they can be compared to astronomical observations. We have implemented two methods for performing halo finding, a key part of the knowledge discovery process, on parallel machines. One of these is a parallel implementation of the friends of friends (FOF) algorithm, widely used in the field of N-body cosmology. The new isodensity (ID) method has been developed to overcome some of the shortcomings of FOR Both have been implemented on a variety of computer systems, and successfully used to extract halos from simulations with up to 256{sup 3} (or about 16.8 million) particles, which axe among the largest N-body cosmology simulations in existence.

  4. rVISTA for Comparative Sequence-Based Discovery of Functional Transcription Factor Binding Sites

    SciTech Connect

    Loots, Gabriela G.; Ovcharenko, Ivan; Pachter, Lior; Dubchak, Inna; Rubin, Edward M.

    2002-03-08

    Identifying transcriptional regulatory elements represents a significant challenge in annotating the genomes of higher vertebrates. We have developed a computational tool, rVISTA, for high-throughput discovery of cis-regulatory elements that combines transcription factor binding site prediction and the analysis of inter-species sequence conservation. Here, we illustrate the ability of rVISTA to identify true transcription factor binding sites through the analysis of AP-1 and NFAT binding sites in the 1 Mb well-annotated cytokine gene cluster1 (Hs5q31; Mm11). The exploitation of orthologous human-mouse data set resulted in the elimination of 95 percent of the 38,000 binding sites predicted upon analysis of the human sequence alone, while it identified 87 percent of the experimentally verified binding sites in this region.

  5. 10 CFR 850 Notice of Proposed Rulemaking Public Hearing Transcript, Richland, Washington – June 28-30, 2016

    Energy.gov [DOE]

    Transcript of the Richland, WA public hearings to received comments on the proposed amendments to 10 CFR 850, Chronic Beryllium Disease Prevention Program.

  6. Role of a Transcriptional Regulator in Programmed Cell Death and Plant Development

    SciTech Connect

    Julie M. Stone

    2008-09-13

    The long-term goal of this research is to understand the role(s) and molecular mechanisms of programmed cell death (PCD) in the controlling plant growth, development and responses to biotic and abiotic stress. We developed a genetic selection scheme to identify A. thaliana FB1-resistant (fbr) mutants as a way to find genes involved in PCD (Stone et al., 2000; Stone et al., 2005; Khan and Stone, 2008). The disrupted gene in fbr6 (AtSPL14) responsible for the FB1-insensitivity and plant architecture phenotypes encodes a plant-specific SBP DNA-binding domain transcriptional regulator (Stone et al., 2005; Liang et al., 2008). This research plan is designed to fill gaps in the knowledge about the role of SPL14 in plant growth and development. The work is being guided by three objectives aimed at determining the pathways in which SPL14 functions to modulate PCD and/or plant development: (1) determine how SPL14 functions in plant development, (2) identify target genes that are directly regulated by SPL14, and (3) identify SPL14 modifications and interacting proteins. We made significant progress during the funding period. Briefly, some major accomplishments are highlighted below: (1) To identify potential AtSPL14 target genes, we identified a consensus DNA binding site for the AtSPL14 SBP DNA-binding domain using systematic evolution of ligands by exponential selection (SELEX) and site-directed mutagenesis (Liang et al., 2008). This consensus binding site was used to analyze Affymetrix microarray gene expression data obtained from wild-type and fbr6 mutant plants to find possible AtSPL14-regulated genes. These candidate AtSPL14-regulated genes are providing new information on the molecular mechanisms linking plant PCD and plant development through modulation of the 26S proteasome. (2) Transgenic plants expressing epitope-tagged versions of AtSPL14 are being used to confirm the AtSPL14 targets (by ChIP-PCR) and further dissect the molecular interactions (Nazarenus, Liang

  7. Energy economists unite. [International Association of Energy Economists findings

    SciTech Connect

    Not Available

    1983-08-10

    Three years after the Arab Oil Embargo, the International Association of Energy Economists (IAEE) was organized. A non-profit organization operated through a council of 15 elected and appointed members, IAEE has already attracted some 1400 individual members from around the world as well as numerous affiliates among national energy organizations. In this issue, Energy Detente reports various IAEE findings, including: (1) a possible 1% growth during 1983 in total energy demand in capitalist countries to be satisfied by coal, nuclear, gas, and hydro supplies, not petroleum; petroleum is expected to figure in the expected 4% growth in energy demand during 1984; (2) usable commercial inventory of petroleum, having risen to 27 days of forward demand by the second quarter of 1981, now sits at 10 days; (3) the current cushion between oil supply and demand, now 15-million barrels per day (b/d) on the side of surplus production capacity during the first quarter of 1983, is exerting downward pressure on prices; and (4) possibly only half the effect of conservation provoked by the second oil-price shock has yet been manifested. If prices fall below US $25 bbl, the second half might be deferred indefinitely, and some enhanced-recovery and frontier-recovery projects would become uneconomic. Also, with OPEC prices holding through the summer of 1983, there is a good chance prices could stabilize for the next several years. This issue presents the Energy Detente fuel price/tax series and the principal industrial fuel prices for August 1983 for countries of the Eastern Hemisphere.

  8. Chk2 regulates transcription-independent p53-mediated apoptosis in response to DNA damage

    SciTech Connect

    Chen Chen [Department of Geriatric Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522 (Japan); Shimizu, Shigeomi [Department of Post-Genomics Diseases, Osaka University Medical School, Suita, Osaka 565-0871 (Japan); Tsujimoto, Yoshihide [Department of Post-Genomics Diseases, Osaka University Medical School, Suita, Osaka 565-0871 (Japan); Motoyama, Noboru [Department of Geriatric Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522 (Japan)]. E-mail: motoyama@nils.go.jp

    2005-07-29

    The tumor suppressor protein p53 plays a central role in the induction of apoptosis in response to genotoxic stress. The protein kinase Chk2 is an important regulator of p53 function in mammalian cells exposed to ionizing radiation (IR). Cells derived from Chk2-deficient mice are resistant to the induction of apoptosis by IR, and this resistance has been thought to be a result of the defective transcriptional activation of p53 target genes. It was recently shown, however, that p53 itself and histone H1.2 translocate to mitochondria and thereby induces apoptosis in a transcription-independent manner in response to IR. We have now examined whether Chk2 also regulates the transcription-independent induction of apoptosis by p53 and histone H1.2. The reduced ability of IR to induce p53 stabilization in Chk2-deficient thymocytes was associated with a marked impairment of p53 and histone H1 translocation to mitochondria. These results suggest that Chk2 regulates the transcription-independent mechanism of p53-mediated apoptosis by inducing stabilization of p53 in response to IR.

  9. Transcriptional responses of Arabidopsis thaliana to chewing and sucking insect herbivores

    SciTech Connect

    Appel, Heidi M.; Fescemyer, Howard; Ehlting, Juergen; Weston, David; Rehrig, Erin; Joshi, Trupti; Xu, Dong; Bohlmann, Joerg; Schultz, Jack

    2014-11-14

    We tested the hypothesis that Arabidopsis can recognize and respond differentially to insect species at the transcriptional level using a genome wide microarray. Transcriptional reprogramming was characterized using co-expression analysis in damaged and undamaged leaves at two times in response to mechanical wounding and four insect species. In all, 2778 (10.6%) of annotated genes on the array were differentially expressed in at least one treatment. Responses differed mainly between aphid and caterpillar and sampling times. Responses to aphids and caterpillars shared only 10% of up-regulated and 8% of down-regulated genes. Responses to two caterpillars shared 21 and 12% of up- and down-regulated genes, whereas responses to the two aphids shared only 7 and 4% of up-regulated and down-regulated genes. Overlap in genes expressed between 6 and 24 h was 3–15%, and depended on the insect species. Responses in attacked and unattacked leaves differed at 6 h but converged by 24 h. Genes responding to the insects are also responsive to many stressors and included primary metabolism. Aphids down-regulated amino acid catabolism; caterpillars stimulated production of amino acids involved in glucosinolate synthesis. Co-expression analysis revealed 17 response networks. Transcription factors were a major portion of differentially expressed genes throughout and responsive genes shared most of the known or postulated binding sites. However, cis-element composition of genes down regulated by the aphid M. persicae was unique, as were those of genes down-regulated by caterpillars. As many as 20 cis-elements were over-represented in one or more treatments, including some from well-characterized classes and others as yet uncharacterized. We suggest that transcriptional changes elicited by wounding and insects are heavily influenced by transcription factors and involve both enrichment of a common set of cis-elements and a unique enrichment of a few cis-elements in responding genes.

  10. Transcriptional responses of Arabidopsis thaliana to chewing and sucking insect herbivores

    DOE PAGES [OSTI]

    Appel, Heidi M.; Fescemyer, Howard; Ehlting, Juergen; Weston, David; Rehrig, Erin; Joshi, Trupti; Xu, Dong; Bohlmann, Joerg; Schultz, Jack

    2014-11-14

    We tested the hypothesis that Arabidopsis can recognize and respond differentially to insect species at the transcriptional level using a genome wide microarray. Transcriptional reprogramming was characterized using co-expression analysis in damaged and undamaged leaves at two times in response to mechanical wounding and four insect species. In all, 2778 (10.6%) of annotated genes on the array were differentially expressed in at least one treatment. Responses differed mainly between aphid and caterpillar and sampling times. Responses to aphids and caterpillars shared only 10% of up-regulated and 8% of down-regulated genes. Responses to two caterpillars shared 21 and 12% of up-more » and down-regulated genes, whereas responses to the two aphids shared only 7 and 4% of up-regulated and down-regulated genes. Overlap in genes expressed between 6 and 24 h was 3–15%, and depended on the insect species. Responses in attacked and unattacked leaves differed at 6 h but converged by 24 h. Genes responding to the insects are also responsive to many stressors and included primary metabolism. Aphids down-regulated amino acid catabolism; caterpillars stimulated production of amino acids involved in glucosinolate synthesis. Co-expression analysis revealed 17 response networks. Transcription factors were a major portion of differentially expressed genes throughout and responsive genes shared most of the known or postulated binding sites. However, cis-element composition of genes down regulated by the aphid M. persicae was unique, as were those of genes down-regulated by caterpillars. As many as 20 cis-elements were over-represented in one or more treatments, including some from well-characterized classes and others as yet uncharacterized. We suggest that transcriptional changes elicited by wounding and insects are heavily influenced by transcription factors and involve both enrichment of a common set of cis-elements and a unique enrichment of a few cis-elements in responding

  11. Histone H4 Lys 20 methyltransferase SET8 promotes androgen receptor-mediated transcription activation in prostate cancer

    SciTech Connect

    Yao, Lushuai; Li, Yanyan; Du, Fengxia; Han, Xiao; Li, Xiaohua; Niu, Yuanjie; Ren, Shancheng; Sun, Yingli

    2014-07-18

    Highlights: • Dihydrotestosterone stimulates H4K20me1 enrichment at the PSA promoter. • SET8 promotes AR-mediated transcription activation. • SET8 interacts with AR and promotes cell proliferation. - Abstract: Histone methylation status in different lysine residues has an important role in transcription regulation. The effect of H4K20 monomethylation (H4K20me1) on androgen receptor (AR)-mediated gene transcription remains unclear. Here we show that AR agonist stimulates the enrichment of H4K20me1 and SET8 at the promoter of AR target gene PSA in an AR dependent manner. Furthermore, SET8 is crucial for the transcription activation of PSA. Co-immunoprecipitation analyses demonstrate that SET8 interacts with AR. Therefore, we conclude that SET8 is involved in AR-mediated transcription activation, possibly through its interaction with AR and H4K20me1 modification.

  12. EA-1761: Finding of No Significant Impact and Floodplain Statement of

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Findings | Department of Energy 1: Finding of No Significant Impact and Floodplain Statement of Findings EA-1761: Finding of No Significant Impact and Floodplain Statement of Findings Clemson University Wind Turbine Drivetrain Test Facility North Charleston, South Carolina The Department of Energy is proposing to provide federal funding to Clemson University to design, permit, and construct a Wind Turbine Drivetrain Test Facility. Finding of No Significant Impact and Floodplain Statement of

  13. Low concentrations of bisphenol a suppress thyroid hormone receptor transcription through a nongenomic mechanism

    SciTech Connect

    Sheng, Zhi-Guo; Tang, Yuan; Liu, Yu-Xiang; Yuan, Ye; Zhao, Bao-Quan; Chao, Xi-Juan; Zhu, Ben-Zhan

    2012-02-15

    Bisphenol (BPA) is one of the highest-volume chemicals produced worldwide, and human exposure to BPA is thought to be ubiquitous. Various rodent and in vitro studies have shown that thyroid hormone (TH) function can be impaired by BPA. However, it is still unknown if low concentrations of BPA can suppress the thyroid hormone receptor (TR) transcription. The present study aims to investigate the possible suppressing effects of low concentrations of BPA on TR transcription and the involved mechanism(s) in CV-1 cells derived from cercopithecus aethiops monkey kidneys. Using gene reporter assays, BPA at concentrations as low as 10{sup ?9} M suppresses TR or steroid receptor coactivator-1(SRC-1)-enhanced TR transcription, but not reducing TR/SRC-1 interaction in mammalian two-hybrid and glutathione S-transferase pull-down studies. It has been further shown that both nuclear receptor co-repressor (N-CoR) and silencing mediator for retinoid and thyroid hormone receptors (SMRT) are recruited to the TR-?1 by BPA in the presence of physiologic concentrations of T3 or T4. However, the overexpression of ?3 integrin or c-Src significantly reduces BPA-induced recruitment of N-CoR/SMRT to TR or suppression of TR transcription. Furthermore, BPA inhibits the T3/T4-mediated interassociation of the ?3 integrin/c-Src/MAPK/TR-?1 pathways by the co-immunoprecipitation. These results indicate that low concentrations of BPA suppress the TR transcription by disrupting physiologic concentrations of T3/T4-mediated ?3 integrin/c-Src/MAPK/TR-?1 pathways, followed by recruiting N-CoR/SMRT to TR-?1, providing a novel insight regarding the TH disruption effects of low concentration BPA. -- Highlights: ? Environmentally relevant concentrations of BPA suppress TR transcription. ? BPA recruits the N-CoR/SMRT to TR under the physiologic concentrations of T3/T4. ? BPA disrupts T3/T4-mediated ?3 integrin/c-Src/MAPK/TR-?1 pathways.

  14. EA-1774-S1: Final Environmental Assessment and Finding of No...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    4-S1: Final Environmental Assessment and Finding of No Significant Impact EA-1774-S1: Final Environmental Assessment and Finding of No Significant Impact Energy Conservation ...

  15. EM Finds Success with Fixed-Priced Hybrid Contract Approach Benefittin...

    Office of Environmental Management (EM)

    Finds Success with Fixed-Priced Hybrid Contract Approach Benefitting Taxpayers EM Finds Success with Fixed-Priced Hybrid Contract Approach Benefitting Taxpayers January 29, 2014 - ...

  16. Molecular genetic analysis of activation-tagged transcription factors thought to be involved in photomorphogenesis

    SciTech Connect

    Neff, Michael M.

    2011-06-23

    This is a final report for Department of Energy Grant No. DE-FG02-08ER15927 entitled “Molecular Genetic Analysis of Activation-Tagged Transcription Factors Thought to be Involved in Photomorphogenesis”. Based on our preliminary photobiological and genetic analysis of the sob1-D mutant, we hypothesized that OBP3 is a transcription factor involved in both phytochrome and cryptochrome-mediated signal transduction. In addition, we hypothesized that OBP3 is involved in auxin signaling and root development. Based on our preliminary photobiological and genetic analysis of the sob2-D mutant, we also hypothesized that a related gene, LEP, is involved in hormone signaling and seedling development.

  17. Regulation of Cell Fate Determination by Single-Repeat R3 MYB Transcription Factors in Arabidopsis

    SciTech Connect

    Wang, Shucai; Chen, Jay

    2014-01-01

    MYB transcription factors regulate multiple aspects of plant growth and development. Among the large family of MYB transcription factors, single-repeat R3 MYB are characterized by their short sequence (<120 amino acids) consisting largely of the single MYB DNA-binding repeat. In the model plant Arabidopsis, R3 MYBs mediate lateral inhibition during epidermal patterning and are best characterized for their regulatory roles in trichome and root hair development. R3 MYBs act as negative regulators for trichome formation but as positive regulators for root hair development. In this article, we provide a comprehensive review on the role of R3 MYBs in the regulation of cell type specification in the model plant Arabidopsis.

  18. Fungal-specific transcription factor AbPf2 activates pathogenicity in Alternaria brassicicola

    SciTech Connect

    Cho, Yangrae; Ohm, Robin A.; Grigoriev, Igor V.; Srivastava, Akhil

    2012-12-03

    Alternaria brassicicola is a successful saprophyte and necrotrophic plant pathogen. To identify molecular determinants of pathogenicity, we created non-pathogenic mutants of a transcription factor-encoding gene, AbPf2. The frequency and timing of germination and appressorium formation on host plants were similar between the non-pathogenic abpf2 mutants and wild-type A. brassicicola. The mutants were also similar in vitro to wild-type A. brassicicola in terms of vegetative growth, conidium production, and responses to a phytoalexin, reactive oxygen species and osmolites. The hyphae of the mutants grew slowly but did not cause disease symptoms on the surface of host plants. Transcripts of the AbPf2 gene increased exponentially soon after wild-type conidia contacted their host plants . A small amount of AbPf2 protein, as monitored using GFP fusions, was present in young, mature conidia. The protein level decreased during saprophytic growth, but increased and was located primarily in fungal nuclei during pathogenesis. Levels of the proteins and transcripts sharply decreased following colonization of host tissues beyond the initial infection site. When expression of the transcription factor was induced in the wild-type during early pathogenesis, 106 fungal genes were also induced in the wild-type but not in the abpf2 mutants. Notably, 33 of the 106 genes encoded secreted proteins, including eight putative effector proteins. Plants inoculated with abpf2 mutants expressed higher levels of genes associated with photosynthesis, the pentose phosphate pathway and primary metabolism, but lower levels of defense-related genes. Our results suggest that AbPf2 is an important regulator of pathogenesis, but does not affect other cellular processes in A. brassicicola.

  19. Transcript of May 4 Webinar: Understanding the Energy Policy and Regulatory

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Environment | Department of Energy 4 Webinar: Understanding the Energy Policy and Regulatory Environment Transcript of May 4 Webinar: Understanding the Energy Policy and Regulatory Environment Good morning, or good afternoon, wherever you may be and welcome to the fifth webinar of the 2016 DOE's Tribal Renewable Energy Webinar Series, "Understanding the Energy Policy and Regulatory Environment." I'm Randy Manion, today's webinar chair and manager of Western Area Power

  20. Transcript abundance on its own cannot be used to infer fluxes in central metabolism

    SciTech Connect

    Schwender, Jorg; Konig, Christina; Klapperstuck, Matthias; Heinzel, Nicolas; Munz, Eberhard; Hebbelmann, Inga; Hay, Jordan O.; Denolf, Peter; De Bodt, Stefanie; Redestig, Henning; Caestecker, Evelyne; Jakob, Peter M.; Borisjuk, Ljudmilla; Rolletschek, Hardy

    2014-11-28

    An attempt has been made to define the extent to which metabolic flux in central plant metabolism is reflected by changes in the transcriptome and metabolome, based on an analysis of in vitro cultured immature embryos of two oilseed rape (Brassica napus) accessions which contrast for seed lipid accumulation. Metabolic flux analysis (MFA) was used to constrain a flux balance metabolic model which included 671 biochemical and transport reactions within the central metabolism. This highly confident flux information was eventually used for comparative analysis of flux vs. transcript (metabolite). Metabolite profiling succeeded in identifying 79 intermediates within the central metabolism, some of which differed quantitatively between the two accessions and displayed a significant shift corresponding to flux. An RNA-Seq based transcriptome analysis revealed a large number of genes which were differentially transcribed in the two accessions, including some enzymes/proteins active in major metabolic pathways. With a few exceptions, differential activity in the major pathways (glycolysis, TCA cycle, amino acid, and fatty acid synthesis) was not reflected in contrasting abundances of the relevant transcripts. The conclusion was that transcript abundance on its own cannot be used to infer metabolic activity/fluxes in central plant metabolism. Lastly, this limitation needs to be borne in mind in evaluating transcriptome data and designing metabolic engineering experiments.

  1. DNA Recognition by a σ54 Transcriptional Activator from Aquifex aeolicus

    DOE PAGES [OSTI]

    Vidangos, Natasha K.; Heideker, Johanna; Lyubimov, Artem; Lamers, Meindert; Huo, Yixin; Pelton, Jeffrey G.; Ton, Jimmy; Gralla, Jay; Berger, James; Wemmer, David E.

    2014-08-23

    Transcription initiation by bacterial σ54-polymerase requires the action of a transcriptional activator protein. Activators bind sequence-specifically upstream of the transcription initiation site via a DNA-binding domain. The structurally characterized DNA-binding domains from activators all belong to the Factor for Inversion Stimulation (Fis) family of helix-turn-helix DNA-binding proteins. We report here structures of the free and DNA-bound forms of the DNA-binding domain of NtrC4 (4DBD) from Aquifex aeolicus, a member of the NtrC family of σ54 activators. Two NtrC4 binding sites were identified upstream (-145 and -85 base pairs) from the start of the lpxC gene, which is responsible for themore » first committed step in Lipid A biosynthesis. This is the first experimental evidence for σ54 regulation in lpxC expression. 4DBD was crystallized both without DNA and in complex with the -145 binding site. The structures, together with biochemical data, indicate that NtrC4 binds to DNA in a manner that is similar to that of its close homologue, Fis. Ultimately, the greater sequence specificity for the binding of 4DBD relative to Fis seems to arise from a larger number of base specific contacts contributing to affinity than for Fis.« less

  2. Double-stranded RNA transcribed from vector-based oligodeoxynucleotide acts as transcription factor decoy

    SciTech Connect

    Xiao, Xiao; Gang, Yi; Wang, Honghong; Wang, Jiayin; Zhao, Lina; Xu, Li; Liu, Zhiguo

    2015-02-06

    Highlights: • A shRNA vector based transcription factor decoy, VB-ODN, was designed. • VB-ODN for NF-κB inhibited cell viability in HEK293 cells. • VB-ODN inhibited expression of downstream genes of target transcription factors. • VB-ODN may enhance nuclear entry ratio for its feasibility of virus production. - Abstract: In this study, we designed a short hairpin RNA vector-based oligodeoxynucleotide (VB-ODN) carrying transcription factor (TF) consensus sequence which could function as a decoy to block TF activity. Specifically, VB-ODN for Nuclear factor-κB (NF-κB) could inhibit cell viability and decrease downstream gene expression in HEK293 cells without affecting expression of NF-κB itself. The specific binding between VB-ODN produced double-stranded RNA and NF-κB was evidenced by electrophoretic mobility shift assay. Moreover, similar VB-ODNs designed for three other TFs also inhibit their downstream gene expression but not that of themselves. Our study provides a new design of decoy for blocking TF activity.

  3. Transcript abundance on its own cannot be used to infer fluxes in central metabolism

    DOE PAGES [OSTI]

    Schwender, Jorg; Konig, Christina; Klapperstuck, Matthias; Heinzel, Nicolas; Munz, Eberhard; Hebbelmann, Inga; Hay, Jordan O.; Denolf, Peter; De Bodt, Stefanie; Redestig, Henning; et al

    2014-11-28

    An attempt has been made to define the extent to which metabolic flux in central plant metabolism is reflected by changes in the transcriptome and metabolome, based on an analysis of in vitro cultured immature embryos of two oilseed rape (Brassica napus) accessions which contrast for seed lipid accumulation. Metabolic flux analysis (MFA) was used to constrain a flux balance metabolic model which included 671 biochemical and transport reactions within the central metabolism. This highly confident flux information was eventually used for comparative analysis of flux vs. transcript (metabolite). Metabolite profiling succeeded in identifying 79 intermediates within the central metabolism,more » some of which differed quantitatively between the two accessions and displayed a significant shift corresponding to flux. An RNA-Seq based transcriptome analysis revealed a large number of genes which were differentially transcribed in the two accessions, including some enzymes/proteins active in major metabolic pathways. With a few exceptions, differential activity in the major pathways (glycolysis, TCA cycle, amino acid, and fatty acid synthesis) was not reflected in contrasting abundances of the relevant transcripts. The conclusion was that transcript abundance on its own cannot be used to infer metabolic activity/fluxes in central plant metabolism. Lastly, this limitation needs to be borne in mind in evaluating transcriptome data and designing metabolic engineering experiments.« less

  4. CITED2 modulates estrogen receptor transcriptional activity in breast cancer cells

    SciTech Connect

    Lau, Wen Min; Doucet, Michele; Huang, David; Weber, Kristy L.; Kominsky, Scott L.

    2013-07-26

    Highlights: The effects of elevated CITED2 on ER function in breast cancer cells are examined. CITED2 enhances cell growth in the absence of estrogen and presence of tamoxifen. CITED2 functions as a transcriptional co-activator of ER in breast cancer cells. -- Abstract: Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (CITED2) is a member of the CITED family of non-DNA binding transcriptional co-activators of the p300/CBP-mediated transcription complex. Previously, we identified CITED2 as being overexpressed in human breast tumors relative to normal mammary epithelium. Upon further investigation within the estrogen receptor (ER)-positive subset of these breast tumor samples, we found that CITED2 mRNA expression was elevated in those associated with poor survival. In light of this observation, we investigated the effect of elevated CITED2 levels on ER function. While ectopic overexpression of CITED2 in three ER-positive breast cancer cell lines (MCF-7, T47D, and CAMA-1) did not alter cell proliferation in complete media, growth was markedly enhanced in the absence of exogenous estrogen. Correspondingly, cells overexpressing CITED2 demonstrated reduced sensitivity to the growth inhibitory effects of the selective estrogen receptor modulator, 4-hydroxytamoxifen. Subsequent studies revealed that basal ER transcriptional activity was elevated in CITED2-overexpressing cells and was further increased upon the addition of estrogen. Similarly, basal and estrogen-induced expression of the ER-regulated genes trefoil factor 1 (TFF1) and progesterone receptor (PGR) was higher in cells overexpressing CITED2. Concordant with this observation, ChIP analysis revealed higher basal levels of CITED2 localized to the TFF-1 and PGR promoters in cells with ectopic overexpression of CITED2, and these levels were elevated further in response to estrogen stimulation. Taken together, these data indicate that CITED2 functions as a transcriptional co

  5. Sex-related differences in murine hepatic transcriptional and proteomic responses to TCDD

    SciTech Connect

    Prokopec, Stephenie D.; Watson, John D.; Lee, Jamie; Pohjanvirta, Raimo; Boutros, Paul C.

    2015-04-15

    2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is an environmental contaminant that produces myriad toxicities in most mammals. In rodents alone, there is a huge divergence in the toxicological response across species, as well as among different strains within a species. But there are also significant differences between males and females animals of a single strain. These differences are inconsistent across model systems: the severity of toxicity is greater in female rats than males, while male mice and guinea pigs are more sensitive than females. Because the specific events that underlie this difference remain unclear, we characterized the hepatic transcriptional response of adult male and female C57BL/6 mice to 500 μg/kg TCDD at multiple time-points. The transcriptional profile diverged significantly between the sexes. Female mice demonstrated a large number of altered transcripts as early as 6 h following treatment, suggesting a large primary response. Conversely, male animals showed the greatest TCDD-mediated response 144 h following exposure, potentially implicating significant secondary responses. Nr1i3 was statistically significantly induced at all time-points in the sensitive male animals. This mRNA encodes the constitutive androstane receptor (CAR), a transcription factor involved in the regulation of xenobiotic metabolism, lipid metabolism, cell cycle and apoptosis. Surprisingly though, changes at the protein level (aside from the positive control, CYP1A1) were modest, with only FMO3 showing clear induction, and no genes with sex-differences. Thus, while male and female mice show transcriptional differences in their response to TCDD, their association with TCDD-induced toxicities remains unclear. - Highlights: • Differences exist between the toxicity phenotypes to TCDD in male and female mice. • TCDD-mediated transcriptomic differences were identified between the sexes. • Resistant female mice displayed a large, early-onset, transcriptomic response.

  6. Identification and overexpression of a knotted1-like transcription factor in switchgrass (Panicum virgatum L.) for lignocellulosic feedstock improvement

    DOE PAGES [OSTI]

    Wuddineh, Wegi A.; Mazarei, Mitra; Zhang, Ji -Yi; Turner, Geoffrey B.; Sykes, Robert W.; Decker, Stephen R.; Davis, Mark F.; Udvardi, Michael K.; C. Neal Stewart, Jr.

    2016-04-28

    High biomass production and wide adaptation has made switchgrass (Panicum virgatum L.) an important candidate lignocellulosic bioenergy crop. One major limitation of this and other lignocellulosic feedstocks is the recalcitrance of complex carbohydrates to hydrolysis for conversion to biofuels. Lignin is the major contributor to recalcitrance as it limits the accessibility of cell wall carbohydrates to enzymatic breakdown into fermentable sugars. Therefore, genetic manipulation of the lignin biosynthesis pathway is one strategy to reduce recalcitrance. Here, we identified a switchgrass Knotted1 transcription factor, PvKN1, with the aim of genetically engineering switchgrass for reduced biomass recalcitrance for biofuel production. Gene expressionmore » of the endogenous PvKN1 gene was observed to be highest in young inflorescences and stems. Ectopic overexpression of PvKN1 in switchgrass altered growth, especially in early developmental stages. Transgenic lines had reduced expression of most lignin biosynthetic genes accompanied by a reduction in lignin content suggesting the involvement of PvKN1 in the broad regulation of the lignin biosynthesis pathway. Moreover, the reduced expression of the Gibberellin 20-oxidase (GA20ox) gene in tandem with the increased expression of Gibberellin 2-oxidase (GA2ox) genes in transgenic PvKN1 lines suggest that PvKN1 may exert regulatory effects via modulation of GA signaling. Furthermore, overexpression of PvKN1 altered the expression of cellulose and hemicellulose biosynthetic genes and increased sugar release efficiency in transgenic lines. Our findings demonstrated that switchgrass PvKN1 is a putative ortholog of maize KN1 that is linked to plant lignification and cell wall and development traits as a major regulatory gene. Therefore, targeted overexpression of PvKN1 in bioenergy feedstocks may provide one feasible strategy for reducing biomass recalcitrance and simultaneously improving plant growth characteristics.« less

  7. Find Preprints

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Questions? 667-5809 Email Databases arXiv.org - open access eprints in Physics, Mathematics, Computer Science, Quantitative Biology, and Statistics bioRxiv.org - preprint server ...

  8. Find Patents

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    Google Patents - search USPTO patents in a Google interface USPTO Database (United States Patent and Trademark Office) DOE Patents - only patents resulting from DOE-sponsored ...

  9. Find Standards

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    ITSDF B56 standards - from the Industrial Truck Standards Development Foundation MSS NECA NEMA NETA NFPA NIBS National CAD Standard (NCS) NISO standards NQA-1:2015 SAE (selected) ...

  10. Oak Ridge Finds Ways to Remove K-25 Faster, Cheaper | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Finds Ways to Remove K-25 Faster, Cheaper Oak Ridge Finds Ways to Remove K-25 Faster, Cheaper February 1, 2012 - 12:00pm Addthis Oak Ridge Finds Ways to Remove K-25 Faster, Cheaper Oak Ridge Finds Ways to Remove K-25 Faster, Cheaper Oak Ridge Finds Ways to Remove K-25 Faster, Cheaper Oak Ridge Finds Ways to Remove K-25 Faster, Cheaper OAK RIDGE, Tenn. - K-25, once the world's largest building under one roof, reflects less of its former title every day. Due to the partnership between the U.S

  11. The oncoprotein HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote the proliferation of breast cancer cells

    SciTech Connect

    Zhang, Yingyi; Zhao, Yu; Li, Leilei; Shen, Yu; Cai, Xiaoli; Zhang, Xiaodong; Ye, Lihong

    2013-05-03

    Highlights: HBXIP is able to upregulate the expression of PDGFB in breast cancer cells. HBXIP serves as a coactivator of activating transcription factor Sp1. HBXIP stimulates the PDGFB promoter via activating transcription factor Sp1. HBXIP promotes the proliferation of breast cancer cell via upregulating PDGFB. -- Abstract: We have reported that the oncoprotein hepatitis B virus X-interacting protein (HBXIP) acts as a novel transcriptional coactivator to promote proliferation and migration of breast cancer cells. Previously, we showed that HBXIP was able to activate nuclear factor-?B (NF-?B) in breast cancer cells. As an oncogene, the platelet-derived growth factor beta polypeptide (PDGFB) plays crucial roles in carcinogenesis. In the present study, we found that both HBXIP and PDGFB were highly expressed in breast cancer cell lines. Interestingly, HBXIP was able to increase transcriptional activity of NF-?B through PDGFB, suggesting that HBXIP is associated with PDGFB in the cells. Moreover, HBXIP was able to upregulate PDGFB at the levels of mRNA, protein and promoter in the cells. Then, we identified that HBXIP stimulated the promoter of PDGFB through activating transcription factor Sp1. In function, HBXIP enhanced the proliferation of breast cancer cells through PDGFB in vitro. Thus, we conclude that HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote proliferation of breast cancer cells.

  12. Structurally Distinct Polycyclic Aromatic Hydrocarbons Induce Differential Transcriptional Responses in Developing Zebrafish

    SciTech Connect

    Goodale, Britton; Tilton, Susan C.; Corvi, Margaret M.; Wilson, Glenn V.; Janszen, Derek B.; Anderson, Kim A.; Waters, Katrina M.; Tanguay, Robert

    2013-11-01

    Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment as components of fossil fuels and by-products of combustion. These multi-ring chemicals differentially activate the aryl hydrocarbon receptor (AHR) in a structurally dependent manner, and induce toxicity via both AHR-dependent and -independent mechanisms. PAH exposure is known to induce developmental malformations in zebrafish embryos, and recent studies have shown cardiac toxicity induced by compounds with low AHR affinity. Unraveling the potentially diverse molecular mechanisms of PAH toxicity is essential for understanding the hazard posed by complex PAH mixtures present in the environment. We analyzed transcriptional responses to PAH exposure in zebrafish embryos exposed to benz(a)anthracene (BAA), dibenzothiophene (DBT) and pyrene (PYR) at concentrations that induced developmental malformations by 120 h post-fertilization (hpf). Whole genome microarray analysis of mRNA expression at 24 and 48 hpf identified genes that were differentially regulated over time and in response to the three PAH structures. PAH body burdens were analyzed at both time points using GC-MS, and demonstrated differences in PAH uptake into the embryos. This was important for discerning dose-related differences from those that represented unique molecular mechanisms. While BAA misregulated the least number of transcripts, it caused strong induction of cyp1a and other genes known to be downstream of the AHR, which were not induced by the other two PAHs. Analysis of functional roles of misregulated genes and their predicted regulatory transcription factors also distinguished the BAA response from regulatory networks disrupted by DBT and PYR exposure. These results indicate that systems approaches can be used to classify the toxicity of PAHs based on the networks perturbed following exposure, and may provide a path for unraveling the toxicity of complex PAH mixtures.

  13. Structural studies of bacterial transcriptional regulatory proteins by multidimensional heteronuclear NMR

    SciTech Connect

    Volkman, B.F.

    1995-02-01

    Nuclear magnetic resonance spectroscopy was used to elucidate detailed structural information for peptide and protein molecules. A small peptide was designed and synthesized, and its three-dimensional structure was calculated using distance information derived from two-dimensional NMR measurements. The peptide was used to induce antibodies in mice, and the cross-reactivity of the antibodies with a related protein was analyzed with enzyme-linked immunosorbent assays. Two proteins which are involved in regulation of transcription in bacteria were also studied. The ferric uptake regulation (Fur) protein is a metal-dependent repressor which controls iron uptake in bacteria. Two- and three-dimensional NMR techniques, coupled with uniform and selective isotope labeling allowed the nearly complete assignment of the resonances of the metal-binding domain of the Fur protein. NTRC is a transcriptional enhancer binding protein whose N-terminal domain is a {open_quote}receiver domain{close_quote} in the family of {open_quote}two-component{close_quote} regulatory systems. Phosphorylation of the N-terminal domain of NTRC activates the initiation of transcription of aeries encoding proteins involved in nitrogen regulation. Three- and four-dimensional NMR spectroscopy methods have been used to complete the resonance assignments and determine the solution structure of the N-terminal receiver domain of the NTRC protein. Comparison of the solution structure of the NTRC receiver domain with the crystal structures of the homologous protein CheY reveals a very similar fold, with the only significant difference being the position of helix 4 relative to the rest of the protein. The determination of the structure of the NTRC receiver domain is the first step toward understanding a mechanism of signal transduction which is common to many bacterial regulatory systems.

  14. Transcription-Driven Twin Supercoiling of a DNA Loop: A Brownian Dynamics Study

    SciTech Connect

    Mielke, S P; Fink, W H; Krishnan, K; Gronbech-Jensen, N; Benham, C J

    2004-06-30

    The torque generated by RNA polymerase as it tracks along double-stranded DNA can potentially induce long-range structural deformations integral to mechanisms of biological significance in both prokaryotes and eukaryotes. In this report, we introduce a dynamic computer model for investigating this phenomenon. Duplex DNA is represented as a chain of hydrodynamic bends interacting through elastic potentials. The chain, linear when relaxed, is looped to form two open but topologically constrained subdomains. This permits the dynamic introduction of torsional stress via a centrally applied torque. We simulate by Brownian dynamics the 100 {micro}s response of a 477-basepair B-DNA template to the localized torque generated by the prokaryotic transcription ensemble. Following a sharp rise at early times, the distributed twist assumes a nearly constant value in both subdomains, and a succession of supercoiling deformations occurs as superhelical stress is increasingly partitioned to writhe. The magnitude of writhe surpasses that of twist before also leveling off when the structure reaches mechanical equilibrium with the torsional load. Superhelicity is simultaneously right-handed in one subdomain and left-handed in the other. The properties of the chain at the onset of writhing agree well with predictions from theory, and the generated stress is ample for driving secondary structural transitions in physiological DNA. These results suggest that the torsional stress generated by transcription can significantly deform the DNA template over short times. This highlights the potential of transcription and other tracking processes to play a central role in gene regulation, and prompts further investigation of dynamically-generated supercoiling.

  15. Metabolism and expression of RNA polymerase II transcripts in Influenza virus-infected cells

    SciTech Connect

    Katze, M.G.; Krug, R.M.

    1984-10-01

    Influenza virus infection has adverse effects on the metabolism of two representative RNA polymerase II transcripts in chicken embryo fibroblasts, those coding for BETA-actin and for avian leukosis virus (ALV) proteins. Proviral ALV DNA was integrated into host cell DNA by prior infection with ALV. By S1 endonuclease assay, it was confirmed that nuclear ALV transcripts disappeared very early after infection, already decreasing ca. 80% by 1 h postinfection. A plausible explanation for this nuclear degradation is that the viral cap-dependent endonuclease in the nucleas cleaves the 5' ends of new polymerase II transcripts, rendering the resulting decapped RNAs susceptible to hydrolysis by cellular nucleases. Similar stability of cytoplasmic host cell mRNAs was observed in infected HeLa cells, in which the levels of actin mRNA and two HeLa cell mRNAs (pHe 7 and pHe 28) remained at undiminished levels for 3 h of infection and decreased only slightly by 4.5 h postinfection. The cytoplamic actin and pHe 7 mRNAs isolated from infected HeLa cells were shown to be translated in reticulocyte extracts in biro, indicating that host mRNAs were not inactivated by a virus-induced modification. Despite the continued presence of high levels of functional host cell mRNAs, host cell protein synthesis was effectively shut off by about 3 h postinfection in both chicken embryo fibroblasts and HeLa cells. These results are consistent with the establishment of an influenza virus-specific translational system that selectively translates viral and not host mRNAs.

  16. Inhibition of hepatitis B virus (HBV) by LNA-mediated nuclear interference with HBV DNA transcription

    SciTech Connect

    Sun, Zhen; Department of Biochemistry and Molecular Biology, Program in Molecular Cell Biology, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058 ; Xiang, Wenqing; Guo, Yajuan; Chen, Zhi; Liu, Wei; Lu, Daru

    2011-06-10

    Highlights: {yields} LNA-modified oligonucleotides can pass through the plasma membrane of cultured cells even without using transfection machinery. {yields} LNA-modified oligonucleotides passed efficiently across the cell membrane, and lipid-coating facilitated translocation from the cytoplasm to the nucleus. {yields} LNA-oligonucleotide designed to target nuclear HBV DNA efficiently suppresses HBV replication and transcription in cultured hepatic cells. -- Abstract: Silencing target genes with small regulatory RNAs is widely used to investigate gene function and therapeutic drug development. Recently, triplex-based approaches have provided another attractive means to achieve targeted gene regulation and gene manipulation at the molecular and cellular levels. Nuclear entry of oligonucleotides and enhancement of their affinity to the DNA targets are key points of such approaches. In this study, we developed lipid-based transport of a locked-nucleic-acid (LNA)-modified oligonucleotide for hepatitis B virus (HBV) DNA interference in human hepatocytes expressing HBV genomic DNA. In these cells, the LNA-modified oligonucleotides passed efficiently across the cell membrane, and lipid-coating facilitated translocation from the cytoplasm to the nucleus. The oligonucleotide specifically targeting HBV DNA clearly interfered with HBV DNA transcription as shown by a block in pregenomic RNA (pgRNA) production. The HBV DNA-targeted oligonucleotide suppressed HBV DNA replication and HBV protein production more efficiently than small interfering RNAs directed to the pgRNA. These results demonstrate that fusion with lipid can carry LNA-modified oligonucleotides to the nucleus where they regulate gene expression. Interfering with HBV DNA transcription by LNA-modified oligonucleotides has strong potential as a new strategy for HBV inhibition.

  17. Transcription Factor Amr1 Induces Melanin Biosynthesis and Suppresses Virulence in Alternaria brassicicola

    SciTech Connect

    Cho, Yangrae; Srivastava, Akhil; Ohm, Robin A.; Lawrence, Christopher B.; Wang, Koon-Hui; Grigoriev, Igor V.; Marahatta, Sharadchandra P.

    2012-05-01

    Alternaria brassicicola is a successful saprophyte and necrotrophic plant pathogen. Several A. brassicicola genes have been characterized as affecting pathogenesis of Brassica species. To study regulatory mechanisms of pathogenesis, we mined 421 genes in silico encoding putative transcription factors in a machine-annotated, draft genome sequence of A. brassicicola. In this study, targeted gene disruption mutants for 117 of the transcription factor genes were produced and screened. Three of these genes were associated with pathogenesis. Disruption mutants of one gene (AbPacC) were nonpathogenic and another gene (AbVf8) caused lesions less than half the diameter of wild-type lesions. Unexpectedly, mutants of the third gene, Amr1, caused lesions with a two-fold larger diameter than the wild type and complementation mutants. Amr1 is a homolog of Cmr1, a transcription factor that regulates melanin biosynthesis in several fungi. We created gene deletion mutants of ?amr1 and characterized their phenotypes. The ?amr1 mutants used pectin as a carbon source more efficiently than the wild type, were melanin-deficient, and more sensitive to UV light and glucanase digestion. The AMR1 protein was localized in the nuclei of hyphae and in highly melanized conidia during the late stage of plant pathogenesis. RNA-seq analysis revealed that three genes in the melanin biosynthesis pathway, along with the deleted Amr1 gene, were expressed at low levels in the mutants. In contrast, many hydrolytic enzyme-coding genes were expressed at higher levels in the mutants than in the wild type during pathogenesis. The results of this study suggested that a gene important for survival in nature negatively affected virulence, probably by a less efficient use of plant cell-wall materials. We speculate that the functions of the Amr1 gene are important to the success of A. brassicicola as a competitive saprophyte and plant parasite.

  18. Human mitochondrial transcription factor A functions in both nuclei and mitochondria and regulates cancer cell growth

    SciTech Connect

    Han, Bin; Izumi, Hiroto; Yasuniwa, Yoshihiro; Akiyama, Masaki; Yamaguchi, Takahiro; Fujimoto, Naohiro; Matsumoto, Tetsuro; Wu, Bin; Tanimoto, Akihide; Sasaguri, Yasuyuki; Kohno, Kimitoshi

    2011-04-29

    Highlights: {yields} Mitochondrial transcription factor A (mtTFA) localizes in nuclei and binds tightly to the nuclear chromatin. {yields} mtTFA contains two putative nuclear localization signals (NLS) in the HMG-boxes. {yields} Overexpression of mtTFA enhances the growth of cancer cells, whereas downregulation of mtTFA inhibits their growth by regulating mtTFA target genes, such as baculoviral IAP repeat-containing 5 (BIRC5; also known as survivin). {yields} Knockdown of mtTFA expression induces p21-dependent G1 cell cycle arrest. -- Abstract: Mitochondrial transcription factor A (mtTFA) is one of the high mobility group protein family and is required for both transcription from and maintenance of mitochondrial genomes. However, the roles of mtTFA have not been extensively studied in cancer cells. Here, we firstly reported the nuclear localization of mtTFA. The proportion of nuclear-localized mtTFA varied among different cancer cells. Some mtTFA binds tightly to the nuclear chromatin. DNA microarray and chromatin immunoprecipitation assays showed that mtTFA can regulate the expression of nuclear genes. Overexpression of mtTFA enhanced the growth of cancer cell lines, whereas downregulation of mtTFA inhibited their growth by regulating mtTFA target genes, such as baculoviral IAP repeat-containing 5 (BIRC5; also known as survivin). Knockdown of mtTFA expression induced p21-dependent G1 cell cycle arrest. These results imply that mtTFA functions in both nuclei and mitochondria to promote cell growth.

  19. Light-up and FRET aptamer reporters; evaluating their applications for imaging transcription in eukaryotic cells

    SciTech Connect

    Ilgu, Muslum; Ray, Judhajeet; Bendickson, Lee; Wang, Tianjiao; Geraskin, Ivan M.; Kraus, George A.; Nilsen-Hamilton, Marit

    2015-12-17

    The regulation of RNA transcription is central to cellular function. Changes in gene expression drive differentiation and cellular responses to events such as injury. RNA trafficking can also have a large impact on protein expression and its localization. Thus, the ability to image RNA transcription and trafficking in real time and in living cells is a worthwhile goal that has been difficult to achieve. The availability of “light-up” aptamers that cause an increase in fluorescence of their ligands when bound by the aptamer have shown promise for reporting on RNA production and localization in vivo. Here we have investigated two light-up aptamers (the malachite green aptamer and the Spinach aptamers) for their suitabilities as reporters of RNA expression in vivo using two eukaryotic cell types, yeast and mammalian. Our analysis focused on the aptamer ligands, their contributions to background noise, and the impact of tandem aptamer strings on signal strength and ligand affinity. Whereas the background fluorescence is very low in vitro, this is not always true for cell imaging. Our results suggest the need for caution in using light-up aptamers as reporters for imaging RNA. In particular, images should be collected and analyzed by operators blinded to the sample identities. The appropriate control condition of ligand with the cells in the absence of aptamer expression must be included in each experiment. This control condition establishes that the specific interaction of ligand with aptamer, rather than nonspecific interactions with unknown cell elements, is responsible for the observed fluorescent signals. As a result, high background signals due to nonspecific interactions of aptamer ligands with cell components can be minimized by using IMAGEtags (Intracellular Multiaptamer GEnetic tags), which signal by FRET and are promising RNA reporters for imaging transcription.

  20. Light-up and FRET aptamer reporters; evaluating their applications for imaging transcription in eukaryotic cells

    DOE PAGES [OSTI]

    Ilgu, Muslum; Ray, Judhajeet; Bendickson, Lee; Wang, Tianjiao; Geraskin, Ivan M.; Kraus, George A.; Nilsen-Hamilton, Marit

    2015-12-17

    The regulation of RNA transcription is central to cellular function. Changes in gene expression drive differentiation and cellular responses to events such as injury. RNA trafficking can also have a large impact on protein expression and its localization. Thus, the ability to image RNA transcription and trafficking in real time and in living cells is a worthwhile goal that has been difficult to achieve. The availability of “light-up” aptamers that cause an increase in fluorescence of their ligands when bound by the aptamer have shown promise for reporting on RNA production and localization in vivo. Here we have investigated twomore » light-up aptamers (the malachite green aptamer and the Spinach aptamers) for their suitabilities as reporters of RNA expression in vivo using two eukaryotic cell types, yeast and mammalian. Our analysis focused on the aptamer ligands, their contributions to background noise, and the impact of tandem aptamer strings on signal strength and ligand affinity. Whereas the background fluorescence is very low in vitro, this is not always true for cell imaging. Our results suggest the need for caution in using light-up aptamers as reporters for imaging RNA. In particular, images should be collected and analyzed by operators blinded to the sample identities. The appropriate control condition of ligand with the cells in the absence of aptamer expression must be included in each experiment. This control condition establishes that the specific interaction of ligand with aptamer, rather than nonspecific interactions with unknown cell elements, is responsible for the observed fluorescent signals. As a result, high background signals due to nonspecific interactions of aptamer ligands with cell components can be minimized by using IMAGEtags (Intracellular Multiaptamer GEnetic tags), which signal by FRET and are promising RNA reporters for imaging transcription.« less

  1. Fact #872: May 11, 2015 Study Finds More than 60% of Millennials...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Car Dealer While Less than Half of Baby Boomers Did - Dataset Fact 872: May 11, 2015 Study Finds More than 60% of Millennials and Generation Xers Use the Internet to Find a Car ...

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    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

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    Office of Science (SC)

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    Office of Environmental Management (EM)

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    Energy Saver

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    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

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    OpenEI (Open Energy Information) [EERE & EIA]

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    U.S. Department of Energy (DOE) - all webpages (Extended Search)

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  10. Structurally distinct polycyclic aromatic hydrocarbons induce differential transcriptional responses in developing zebrafish

    SciTech Connect

    Goodale, Britton C.; Tilton, Susan C.; Corvi, Margaret M.; Wilson, Glenn R.; Janszen, Derek B.; Anderson, Kim A.; Waters, Katrina M.; Tanguay, Robert L.

    2013-11-01

    Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment as components of fossil fuels and by-products of combustion. These multi-ring chemicals differentially activate the aryl hydrocarbon receptor (AHR) in a structurally dependent manner, and induce toxicity via both AHR-dependent and -independent mechanisms. PAH exposure is known to induce developmental malformations in zebrafish embryos, and recent studies have shown cardiac toxicity induced by compounds with low AHR affinity. Unraveling the potentially diverse molecular mechanisms of PAH toxicity is essential for understanding the hazard posed by complex PAH mixtures present in the environment. We analyzed transcriptional responses to PAH exposure in zebrafish embryos exposed to benz(a)anthracene (BAA), dibenzothiophene (DBT) and pyrene (PYR) at concentrations that induced developmental malformations by 120 h post-fertilization (hpf). Whole genome microarray analysis of mRNA expression at 24 and 48 hpf identified genes that were differentially regulated over time and in response to the three PAH structures. PAH body burdens were analyzed at both time points using GC–MS, and demonstrated differences in PAH uptake into the embryos. This was important for discerning dose-related differences from those that represented unique molecular mechanisms. While BAA misregulated the least number of transcripts, it caused strong induction of cyp1a and other genes known to be downstream of the AHR, which were not induced by the other two PAHs. Analysis of functional roles of misregulated genes and their predicted regulatory transcription factors also distinguished the BAA response from regulatory networks disrupted by DBT and PYR exposure. These results indicate that systems approaches can be used to classify the toxicity of PAHs based on the networks perturbed following exposure, and may provide a path for unraveling the toxicity of complex PAH mixtures. - Highlights: • Defined global mRNA expression

  11. Microsoft Word - Consolidated Final Complete Transcript CBS Meeting Boston_Final v1

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Hyatt Regency Boston One Avenue De Lafayette Boston, MA 02111 June 2, 2016 FULL TRANSCRIPT Mr. Jim Hamilton. Good afternoon. Welcome to Boston, and to the fifth in a series of public meetings the Department is hosting on its Consent-Based Siting Program. Thank you all for being here today. I'm especially happy to welcome you for two reasons. One is, I live in Boston, so, welcome to my hometown. But more importantly, we've got an excellent panel here in front of you here today, and I'm looking

  12. Microsoft Word - Consolidated Full Transcript of DOE CBS Meeting Minneapolis 21 JUL 16_Final

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    CONSENT-BASED SITING PUBLIC MEETING Hilton Minneapolis 1001 Marquette Avenue Minneapolis, MN 55403 July 21, 2016 FULL TRANSCRIPT Mr. Jim Hamilton. Good afternoon. And for those joining us in later time zones via webinar, good evening. Welcome to Minneapolis and to the eighth in a series of public meetings the Department is hosting on its Consent-Based Siting Program. Thank you all very much for being here today. My name is Jim Hamilton; I'm an advisor to the Department as part of their

  13. Microsoft Word - Final Consolitated Full Transcript Boise CBS Meeting_Final

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    P a g e CONSENT-BASED SITING PUBLIC MEETING Boise Centre 850 West Front Street Boise, ID 83702 July 14, 2016 FULL TRANSCRIPT Mr. Jim Hamilton. Good afternoon, and for those of you joining us in a later time zone, through the webinar, good evening. Welcome to Boise, and to the seventh in a series of public meetings the Department is hosting on its Consent-Based Siting Program. Thank you all for being here today. My name is Jim Hamilton; I'm an advisor to the Department as part of its

  14. ECRbase: Database of Evolutionary Conserved Regions, Promoters, and Transcription Factor Binding Sites in Vertebrate Genomes

    DOE Data Explorer

    Loots, Gabriela G. [LLNL; Ovcharenko, I. [LLNL

    Evolutionary conservation of DNA sequences provides a tool for the identification of functional elements in genomes. This database of evolutionary conserved regions (ECRs) in vertebrate genomes features a database of syntenic blocks that recapitulate the evolution of rearrangements in vertebrates and a comprehensive collection of promoters in all vertebrate genomes generated using multiple sources of gene annotation. The database also contains a collection of annotated transcription factor binding sites (TFBSs) in evolutionary conserved and promoter elements. ECRbase currently includes human, rhesus macaque, dog, opossum, rat, mouse, chicken, frog, zebrafish, and fugu genomes. (taken from paper in Journal: Bioinformatics, November 7, 2006, pp. 122-124

  15. Transcript of the Energy Data Management Webinar Series - Part 1: Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Data Collection | Department of Energy the Energy Data Management Webinar Series - Part 1: Energy Data Collection Transcript of the Energy Data Management Webinar Series - Part 1: Energy Data Collection Crystal McDonald: Good afternoon, everyone. My name is Crystal McDonald. I'm a policy advisor on the staff of the Weatherization and Intergovernmental Programs Office, the Policy and Technical Assistance Team. Welcome to our webinar today. We're happy to have you, and we will be presenting

  16. Report Summary: Hammer Testing Findings for Solid-State Lighting Luminaires

    SciTech Connect

    2014-01-01

    Brief summary of DOE LED Systems Reliability Consortium's Hammer Testing Findings for Solid-State Lighting Luminaires report.

  17. Summary of Findings: Peer Review of the FY2001 GPRA Assumptions

    Energy.gov [DOE]

    Summary of Findings: Peer Review of the FY2001 GPRA Assumptions, Report to National Renewable Energy Laboratory, February 29, 2000.

  18. EA-1945: Finding of No Significant Impact and Mitigation Action Plan |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy Finding of No Significant Impact and Mitigation Action Plan EA-1945: Finding of No Significant Impact and Mitigation Action Plan Northern Mid-Columbia Joint Project; Grant, Douglas, and Chelan Counties, Washington Bonneville Power Administration (BPA) issued a finding of no significant impact, which includes a floodplain statement of findings, for the proposed Northern Mid-Columbia Joint Project, a 230-kilvolt transmission line proposed by BPA and the Public Utility

  19. Summary of Findings: Peer Review of the FY2000 GPRA Assumptions

    Energy.gov [DOE]

    Summary of Findings: Peer Review of the FY2000 GPRA Assumptions, Report to National Renewable Energy Laboratory, March 1999.

  20. Fact #872: May 11, 2015 Study Finds More than 60% of Millennials and

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Generation Xers Use the Internet to Find a Car Dealer While Less than Half of Baby Boomers Did | Department of Energy 2: May 11, 2015 Study Finds More than 60% of Millennials and Generation Xers Use the Internet to Find a Car Dealer While Less than Half of Baby Boomers Did Fact #872: May 11, 2015 Study Finds More than 60% of Millennials and Generation Xers Use the Internet to Find a Car Dealer While Less than Half of Baby Boomers Did According to an AutoTrader-commissioned study of people

  1. Crystal structure of enterococcus faecalis sly A-like transcriptional factor.

    SciTech Connect

    Wu, R.; Zhang, R.; Zagnitko, O.; Dementieva, I.; Maltsev, N.; Watson, J. D.; Laskowski, R.; Gornicki, P.; Joachimiak, A.; Univ. of Chicago; European Bioinformatics Inst.

    2003-05-30

    The crystal structure of a SlyA transcriptional regulator at 1.6 {angstrom} resolution is presented, and structural relationships between members of the MarR/SlyA family are discussed. The SlyA family, which includes SlyA, Rap, Hor, and RovA proteins, is widely distributed in bacterial and archaeal genomes. Current evidence suggests that SlyA-like factors act as repressors, activators, and modulators of gene transcription. These proteins have been shown to up-regulate the expression of molecular chaperones, acid-resistance proteins, and cytolysin, and down-regulate several biosynthetic enzymes. The structure of SlyA from Enterococcus faecalis, determined as a part of an ongoing structural genomics initiative (www.mcsg.anl.gov), revealed the same winged helix DNA-binding motif that was recently found in the MarR repressor from Escherichia coli and the MexR repressor from Pseudomonas aeruginosa, a sequence homologue of MarR. Phylogenetic analysis of the MarR/SlyA family suggests that Sly is placed between the SlyA and MarR subfamilies and shows significant sequence similarity to members of both subfamilies.

  2. mRNA Transcript Abundance during Plant Growth and the Influence of Li+ Exposure

    DOE PAGES [OSTI]

    Duff, M. C.; Kuhne, W. W.; Halverson, N. V.; Chang, C. -S.; Kitamura, E.; Hawthorn, L.; Martinez, N. E.; Stafford, C.; Milliken, C. E.; Caldwell, E. F.; et al

    2014-10-23

    Lithium (Li) toxicity in plants is, at a minimum, a function of Li+ concentration, exposure time, species and growth conditions. Most plant studies with Li+ focus on short-term acute exposures. This study examines short- and long-term effects of Li+ exposure in Arabidopsis with Li+ uptake studies and measured shoot mRNA transcript abundance levels in treated and control plants. Stress, pathogen-response and arabinogalactan protein genes were typically more up-regulated in older (chronic, low level) Li+-treatment plants and in the much younger plants from acute high-level exposures. The gene regulation behavior of high-level Li+ resembled prior studies due to its influence on:more »inositol synthesis, 1-aminocyclopropane-1-carboxylate synthases and membrane ion transport. In contrast, chronically-exposed plants had gene regulation responses that were indicative of pathogen, cold, and heavy-metal stress, cell wall degradation, ethylene production, signal transduction, and calcium-release modulation. Acute Li+ exposure phenocopies magnesium-deficiency symptoms and is associated with elevated expression of stress response genes that could lead to consumption of metabolic and transcriptional energy reserves and the dedication of more resources to cell development. In contrast, chronic Li+ exposure increases expression signal transduction genes. The identification of new Li+-sensitive genes and a gene-based “response plan” for acute and chronic Li+ exposure are delineated.« less

  3. mRNA Transcript Abundance during Plant Growth and the Influence of Li+ Exposure

    DOE PAGES [OSTI]

    Duff, M. C.; Kuhne, W. W.; Halverson, N. V.; Chang, C. -S.; Kitamura, E.; Hawthorn, L.; Martinez, N. E.; Stafford, C.; Milliken, C. E.; Caldwell, E. F.; et al

    2014-10-23

    Lithium (Li) toxicity in plants is, at a minimum, a function of Li+ concentration, exposure time, species and growth conditions. Most plant studies with Li+ focus on short-term acute exposures. This study examines short- and long-term effects of Li+ exposure in Arabidopsis with Li+ uptake studies and measured shoot mRNA transcript abundance levels in treated and control plants. Stress, pathogen-response and arabinogalactan protein genes were typically more up-regulated in older (chronic, low level) Li+-treatment plants and in the much younger plants from acute high-level exposures. The gene regulation behavior of high-level Li+ resembled prior studies due to its influence on:more » inositol synthesis, 1-aminocyclopropane-1-carboxylate synthases and membrane ion transport. In contrast, chronically-exposed plants had gene regulation responses that were indicative of pathogen, cold, and heavy-metal stress, cell wall degradation, ethylene production, signal transduction, and calcium-release modulation. Acute Li+ exposure phenocopies magnesium-deficiency symptoms and is associated with elevated expression of stress response genes that could lead to consumption of metabolic and transcriptional energy reserves and the dedication of more resources to cell development. In contrast, chronic Li+ exposure increases expression signal transduction genes. The identification of new Li+-sensitive genes and a gene-based “response plan” for acute and chronic Li+ exposure are delineated.« less

  4. Reprogramming of human fibroblasts to pluripotent stem cells using mRNA of four transcription factors

    SciTech Connect

    Yakubov, Eduard; Rechavi, Gidi; Rozenblatt, Shmuel; Givol, David

    2010-03-26

    Reprogramming of differentiated cells into induced pluripotent cells (iPS) was accomplished in 2006 by expressing four, or less, embryonic stem cell (ESC)-specific transcription factors. Due to the possible danger of DNA damage and the potential tumorigenicity associated with such DNA damage, attempts were made to minimize DNA integration by the vectors involved in this process without complete success. Here we present a method of using RNA transfection as a tool for reprogramming human fibroblasts to iPS. We used RNA synthesized in vitro from cDNA of the same reprogramming four transcription factors. After transfection of the RNA, we show intracellular expression and nuclear localization of the respective proteins in at least 70% of the cells. We used five consecutive transfections to support continuous protein expression resulting in the formation of iPS colonies that express alkaline phosphatase and several ESC markers and that can be expanded. This method completely avoids DNA integration and may be developed to replace the use of DNA vectors in the formation of iPS.

  5. Extensive cross-regulation of post-transcriptional regulatory networks in Drosophila

    SciTech Connect

    Stoiber, Marcus H.; Olson, Sara; May, Gemma E.; Duff, Michael O.; Manent, Jan; Obar, Robert; Guruharsha, K. G.; Bickel, Peter J.; Artavanis-Tsakonas, Spyros; Brown, James B.; Graveley, Brenton R.; Celniker, Susan E.

    2015-08-20

    In eukaryotic cells, RNAs exist as ribonucleoprotein particles (RNPs). Despite the importance of these complexes in many biological processes, including splicing, polyadenylation, stability, transportation, localization, and translation, their compositions are largely unknown. We affinity-purified 20 distinct RNA-binding proteins (RBPs) from cultured Drosophila melanogaster cells under native conditions and identified both the RNA and protein compositions of these RNP complexes. We identified “high occupancy target” (HOT) RNAs that interact with the majority of the RBPs we surveyed. HOT RNAs encode components of the nonsense-mediated decay and splicing machinery, as well as RNA-binding and translation initiation proteins. The RNP complexes contain proteins and mRNAs involved in RNA binding and post-transcriptional regulation. Genes with the capacity to produce hundreds of mRNA isoforms, ultracomplex genes, interact extensively with heterogeneous nuclear ribonuclear proteins (hnRNPs). Our data are consistent with a model in which subsets of RNPs include mRNA and protein products from the same gene, indicating the widespread existence of auto-regulatory RNPs. Lastly, from the simultaneous acquisition and integrative analysis of protein and RNA constituents of RNPs, we identify extensive cross-regulatory and hierarchical interactions in post-transcriptional control.

  6. Extensive cross-regulation of post-transcriptional regulatory networks in Drosophila

    DOE PAGES [OSTI]

    Stoiber, Marcus H.; Olson, Sara; May, Gemma E.; Duff, Michael O.; Manent, Jan; Obar, Robert; Guruharsha, K. G.; Bickel, Peter J.; Artavanis-Tsakonas, Spyros; Brown, James B.; et al

    2015-08-20

    In eukaryotic cells, RNAs exist as ribonucleoprotein particles (RNPs). Despite the importance of these complexes in many biological processes, including splicing, polyadenylation, stability, transportation, localization, and translation, their compositions are largely unknown. We affinity-purified 20 distinct RNA-binding proteins (RBPs) from cultured Drosophila melanogaster cells under native conditions and identified both the RNA and protein compositions of these RNP complexes. We identified “high occupancy target” (HOT) RNAs that interact with the majority of the RBPs we surveyed. HOT RNAs encode components of the nonsense-mediated decay and splicing machinery, as well as RNA-binding and translation initiation proteins. The RNP complexes contain proteinsmore » and mRNAs involved in RNA binding and post-transcriptional regulation. Genes with the capacity to produce hundreds of mRNA isoforms, ultracomplex genes, interact extensively with heterogeneous nuclear ribonuclear proteins (hnRNPs). Our data are consistent with a model in which subsets of RNPs include mRNA and protein products from the same gene, indicating the widespread existence of auto-regulatory RNPs. Lastly, from the simultaneous acquisition and integrative analysis of protein and RNA constituents of RNPs, we identify extensive cross-regulatory and hierarchical interactions in post-transcriptional control.« less

  7. Whole-genome transcriptional analysis of heavy metal stresses inCaulobacter crescentus

    SciTech Connect

    Hu, Ping; Brodie, Eoin L.; Suzuki, Yohey; McAdams, Harley H.; Andersen, Gary L.

    2005-09-21

    The bacterium Caulobacter crescentus and related stalkbacterial species are known for their distinctive ability to live in lownutrient environments, a characteristic of most heavy metal contaminatedsites. Caulobacter crescentus is a model organism for studying cell cycleregulation with well developed genetics. We have identified the pathwaysresponding to heavy metal toxicity in C. crescentus to provide insightsfor possible application of Caulobacter to environmental restoration. Weexposed C. crescentus cells to four heavy metals (chromium, cadmium,selenium and uranium) and analyzed genome wide transcriptional activitiespost exposure using a Affymetrix GeneChip microarray. C. crescentusshowed surprisingly high tolerance to uranium, a possible mechanism forwhich may be formation of extracellular calcium-uranium-phosphateprecipitates. The principal response to these metals was protectionagainst oxidative stress (up-regulation of manganese-dependent superoxidedismutase, sodA). Glutathione S-transferase, thioredoxin, glutaredoxinsand DNA repair enzymes responded most strongly to cadmium and chromate.The cadmium and chromium stress response also focused on reducing theintracellular metal concentration, with multiple efflux pumps employed toremove cadmium while a sulfate transporter was down-regulated to reducenon-specific uptake of chromium. Membrane proteins were also up-regulatedin response to most of the metals tested. A two-component signaltransduction system involved in the uranium response was identified.Several differentially regulated transcripts from regions previously notknown to encode proteins were identified, demonstrating the advantage ofevaluating the transcriptome using whole genome microarrays.

  8. BFV activates the NF-kappaB pathway through its transactivator (BTas) to enhance viral transcription

    SciTech Connect

    Wang Jian; Tan Juan; Zhang Xihui; Guo Hongyan; Zhang Qicheng; Guo Tingting; Geng Yunqi; Qiao Wentao

    2010-05-10

    Multiple families of viruses have evolved sophisticated strategies to regulate nuclear factor-kappaB (NF-kappaB) signaling, which plays a pivotal role in diverse cellular events, including virus-host interactions. In this study, we report that bovine foamy virus (BFV) is able to activate the NF-kappaB pathway through the action of its transactivator, BTas. Both cellular IKKbeta and IkappaBalpha also participate in this activation. In addition, we demonstrate that BTas induces the processing of p100, which implies that BTas can activate NF-kappaB through a noncanonical pathway as well. Co-immunoprecipitation analysis shows that BTas interacts with IKK catalytic subunits (IKKalpha and IKKbeta), which may be responsible for regulation of IKK kinase activity and persistent NF-kappaB activation. Furthermore, our results indicate that the level of BTas-mediated LTR transcription correlates with the activity of cellular NF-kappaB. Together, this study suggests that BFV activates the NF-kappaB pathway through BTas to enhance viral transcription.

  9. Oestradiol reduces Liver Receptor Homolog-1 mRNA transcript stability in breast cancer cell lines

    SciTech Connect

    Lazarus, Kyren A.; Environmental and Biotechnology Centre, Swinburne University, Hawthorn, Victoria 3122 ; Zhao, Zhe; Knower, Kevin C.; To, Sarah Q.; Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3168 ; Chand, Ashwini L.; Clyne, Colin D.

    2013-08-30

    Highlights: LRH-1 is an orphan nuclear receptor that regulates tumor proliferation. In breast cancer, high mRNA expression is associated with ER+ status. In ER?ve cells, despite very low mRNA, we found abundant LRH-1 protein. Our data show distinctly different LRH-1 protein isoforms in ER? and ER+ breast cancer cells. This is due to differences in LRH-1 mRNA and protein stability rates. -- Abstract: The expression of orphan nuclear receptor Liver Receptor Homolog-1 (LRH-1) is elevated in breast cancer and promotes proliferation, migration and invasion in vitro. LRH-1 expression is regulated by oestrogen (E{sub 2}), with LRH-1 mRNA transcript levels higher in oestrogen receptor ? (ER?) positive (ER+) breast cancer cells compared to ER? cells. However, the presence of LRH-1 protein in ER? cells suggests discordance between mRNA transcript levels and protein expression. To understand this, we investigated the impact of mRNA and protein stability in determining LRH-1 protein levels in breast cancer cells. LRH-1 transcript levels were significantly higher in ER+ versus ER? breast cancer cells lines; however LRH-1 protein was expressed at similar levels. We found LRH-1 mRNA and protein was more stable in ER? compared to ER+ cell lines. The tumor-specific LRH-1 variant isoform, LRH-1v4, which is highly responsive to E{sub 2}, showed increased mRNA stability in ER? versus ER+ cells. In addition, in MCF-7 and T47-D cell lines, LRH-1 total mRNA stability was reduced with E{sub 2} treatment, this effect mediated by ER?. Our data demonstrates that in ER? cells, increased mRNA and protein stability contribute to the abundant protein expression levels. Expression and immunolocalisation of LRH-1 in ER? cells as well as ER? tumors suggests a possible role in the development of ER? tumors. The modulation of LRH-1 bioactivity may therefore be beneficial as a treatment option in both ER? and ER+ breast cancer.

  10. On-chip single-copy real-time reverse-transcription PCR in isolated picoliter droplets

    SciTech Connect

    Beer, N R; Wheeler, E; Lee-Houghton, L; Watkins, N; Nasarabadi, S; Hebert, N; Leung, P; Arnold, D; Bailey, C; Colston, B

    2007-12-19

    The first lab-on-chip system for picoliter droplet generation and RNA isolation, followed by reverse transcription, and PCR amplification with real-time fluorescence detection in the trapped droplets has been developed. The system utilized a shearing T-junction in a fused silica device to generate a stream of monodisperse picoliter-scale droplets that were isolated from the microfluidic channel walls and each other by the oil phase carrier. An off-chip valving system stopped the droplets on-chip, allowing thermal cycling for reverse transcription and subsequent PCR amplification without droplet motion. This combination of the established real-time reverse transcription-PCR assay with digital microfluidics is ideal for isolating single-copy RNA and virions from a complex environment, and will be useful in viral discovery and gene-profiling applications.

  11. Business Case for Small Modular Reactors Report on Findings | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Energy Business Case for Small Modular Reactors Report on Findings Business Case for Small Modular Reactors Report on Findings This study assesses the market for SMRs and develops a business case to identify incentives, policies, and programs that can be effectively implemented and have significant impact on the commercialization of SMRs. Business Case for Small Modular Reactors Report on Findings to the U.S. Department of Energy (666.81 KB) More Documents & Publications A Strategic

  12. Focus Series: Program Finds Community "Readiness" Is the Key to More

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Retrofits | Department of Energy Program Finds Community "Readiness" Is the Key to More Retrofits Focus Series: Program Finds Community "Readiness" Is the Key to More Retrofits Focus Series: Program Finds Community "Readiness" Is the Key to More Retrofits, a document posted by the U.S. Department of Energy's (DOE'S) Better Buildings Neighborhood Program. Focus Series: Michigan (284.83 KB) More Documents & Publications Community Readiness Assessments Better

  13. EA-1950: Finding of No Significant Impact and Mitigation Action Plan |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy 0: Finding of No Significant Impact and Mitigation Action Plan EA-1950: Finding of No Significant Impact and Mitigation Action Plan Grand Coulee-Creston Transmission Line Rebuild; Grant and Lincoln Counties, Washington Bonneville Power Administration issued a finding of no significant impact and a mitigation action plan for rebuilding approximately 28 miles of the Grand Coulee-Creston No. 1 115-kilovolt (kV) transmission line between Coulee Dam in Grant County and

  14. EA-1951: Finding of No Significant Impact and Mitigation Action Plan |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy Finding of No Significant Impact and Mitigation Action Plan EA-1951: Finding of No Significant Impact and Mitigation Action Plan Midway-Moxee Rebuild and Midway-Grandview Upgrade Transmission Line Project; Benton and Yakima Counties, Washington Bonneville Power Administration issued a Finding of No Significant Impact and Mitigation Action Plan for the proposed rebuilding of the 34-mile Midway-Moxee transmission line and the proposed rebuilding and upgrading of the

  15. EA-2006: Finding of No Significant Impact and Mitigation Action Plan |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy Finding of No Significant Impact and Mitigation Action Plan EA-2006: Finding of No Significant Impact and Mitigation Action Plan Columbia Estuary Ecosystem Restoration Program Bonneville Power Administration issued a FONSI and a floodplain and wetland statement of findings for a proposed program of actions to help restore ecological structure, function, and biodiversity within the Columbia River estuary. The mitigation measures listed in the Mitigation Action Plan and

  16. Scientists Find Ocean 'Dead' Zones Teeming with Life | U.S. DOE Office of

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Science (SC) Scientists Find Ocean 'Dead' Zones Teeming with Life News News Home Featured Articles 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 Science Headlines Science Highlights Presentations & Testimony News Archives Communications and Public Affairs Contact Information Office of Science U.S. Department of Energy 1000 Independence Ave., SW Washington, DC 20585 P: (202) 586-5430 11.23.09 Scientists Find Ocean 'Dead' Zones Teeming with Life Scientists find microorganisms

  17. Using regulations.gov to find dockets and documents | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Using regulations.gov to find dockets and documents Using regulations.gov to find dockets and documents A document from the U.S. Department of Energy's Office of Energy Efficiency and Renewable Energy, Building Technologies Office, explaining how to use Regulations.gov to find dockets and documents. as_accessing_documents.pdf (700.83 KB) More Documents & Publications Regulations.gov - Proposed Rule Document ISSUANCE 2015-08-21: Energy Conservation Program for Consumer Products: Energy

  18. Who's got more WorldWideScience? Find out on OSTI's YouTube Channel |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy Office of Scientific and Technical Information Who's got more WorldWideScience? Find out on OSTI's YouTube Channel Back to the OSTI News Listing for 2009 Who's Got More WorldWideScience? Find out on OSTI's YouTube channel, and then explore WorldWideScience.org to find research from more than 60 countries via one search box

  19. Tour of Zero: Helping Customers Find Better Homes at Lower Costs Webinar

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    (Text Version) | Department of Energy Tour of Zero: Helping Customers Find Better Homes at Lower Costs Webinar (Text Version) Tour of Zero: Helping Customers Find Better Homes at Lower Costs Webinar (Text Version) Below is the text version of the webinar Tour of Zero: Helping Customers Find Better Homes at Lower Costs, presented in October 2015. Watch the presentation. Lindsay Parker: Hi, everyone. Welcome to the Department of Energy Zero Energy Ready Home technical training webinar series.

  20. EA-1815: DOE Notice of Availability of the Finding of No Significant...

    Energy.gov [DOE] (indexed site)

    environmental impacts associated with the: Cuyahoga County Agricultural Society Wind Energy Project; Berea, Cuyahoga County, Ohio. Notice of Availability of a Finding of No...