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Sample records for discipline structural biology

  1. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    Biological, and Environmental Engineering, Oregon State University Williams, John M. (John M. Williams) - Petroleum Engineering program, Petroleum Institute (Abu Dhabi) Go ...

  2. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    L M N O P Q R S T U V W X Y Z Keller, Christopher (Christopher Keller) - Department of Biology, Minot State University Go back to Individual Researchers Collections: A B C D E F G ...

  3. Structural Molecular Biology, SSRL

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Molecular Biology Group hosted a 3-day comprehensive workshop on the use of non-crystalline small-angle x-ray scattering and diffraction techniques in structural biology research. ...

  4. Structural Molecular Biology, SSRL

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Our Mission Our Mission The SSRL Structural Molecular Biology program operates as a integrated resource and has three primary areas (or cores) of technological research and ...

  5. Structural Molecular Biology, SSRL

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Toggle navigation SMB Home Mission Facilities Macromolecular Crystallography Small Angle X-Ray Scattering X-Ray Spectroscopy & Imaging User Resources User Resources User Portal Schedules Deadlines Highlights Training Workshops & Summer Schools Summer Students Structural Molecular Biology Illuminating Biological Structures at the Atomic and Molecular Levels Your browser does not support the video tag. SMB Mission SSRL's SMB program operates as an integrated resource and has three cores of

  6. Structural Molecular Biology, SSRL

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Science Highlights Science Highlights Structure of Chinese Herbal-based Medicine Captured by ATP on a Human tRNA Synthetase For approximately 2,000 years, the Chinese have been ...

  7. Report of The Structural Biology Subcommittee of The Biological and

    Office of Science (SC)

    Environmental Research Advisory Committee | U.S. DOE Office of Science (SC) Report of The Structural Biology Subcommittee of The Biological and Environmental Research Advisory Committee Biological and Environmental Research Advisory Committee (BERAC) BERAC Home Meetings Members Charges/Reports Current BERAC Charges Archive of BERAC Reports Charter .pdf file (135KB) BER Committees of Visitors Federal Advisory Committees BER Home Charges/Reports Report of The Structural Biology Subcommittee of

  8. Structural Biology | U.S. DOE Office of Science (SC)

    Office of Science (SC)

    Structural Biology Biological and Environmental Research (BER) BER Home About Research Biological Systems Science Division (BSSD) Genomic Science DOE Bioenergy Research Centers Bioimaging Technology DOE Joint Genome Institute Structural Biology Radiochemistry & Imaging Instrumentation Radiobiology: Low Dose Radiation Research DOE Human Subjects Protection Program Climate and Environmental Sciences Division (CESD) Research Abstracts Searchable Archive of BER Highlights External link

  9. 2010 Diffraction Methods in Structural Biology

    SciTech Connect

    Dr. Ana Gonzalez

    2011-03-10

    Advances in basic methodologies have played a major role in the dramatic progress in macromolecular crystallography over the past decade, both in terms of overall productivity and in the increasing complexity of the systems being successfully tackled. The 2010 Gordon Research Conference on Diffraction Methods in Structural Biology will, as in the past, focus on the most recent developments in methodology, covering all aspects of the process from crystallization to model building and refinement, complemented by examples of structural highlights and complementary methods. Extensive discussion will be encouraged and it is hoped that all attendees will participate by giving oral or poster presentations, the latter using the excellent poster display area available at Bates College. The relatively small size and informal atmosphere of the meeting provides an excellent opportunity for all participants, especially younger scientists, to meet and exchange ideas with leading methods developers.

  10. The Biological Implications of the PP2A Crystal Structure

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    The Biological Implications of the PP2A Crystal Structure Print Phosphatases, enzymes that remove a phosphate group from amino-acid substrates, can be subdivided according to their...

  11. Administering Work Force Discipline

    Directives, Delegations, and Other Requirements [Office of Management (MA)]

    2015-05-14

    The order provides requirements and responsibilities for administering work force discipline and corrective actions. Supersedes DOE O 3750.1.

  12. Electronic Structure and Excited State Dynamics in Biological and Nanoscale

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Systems | MIT-Harvard Center for Excitonics Structure and Excited State Dynamics in Biological and Nanoscale Systems February 25, 2009 at 3pm/36-428 Gregory D. Scholes Department of Chemistry, University of Toronto scholes2 abstract: After photoexcitation, energy absorbed by a molecule can be transferred efficiently over a distance of up to several tens of Ångstrom to another molecule by the process of resonance energy transfer, RET (also commonly known as electronic energy transfer, EET).

  13. Administering Workforce Discipline

    Directives, Delegations, and Other Requirements [Office of Management (MA)]

    2014-10-27

    To provide requirements and responsibilities for administering workforce discipline that includes disciplinary, adverse, and alternative corrective actions in the Department of Energy (DOE).

  14. Work Force Discipline

    Directives, Delegations, and Other Requirements [Office of Management (MA)]

    1983-03-23

    The order provides guidance and procedures and states responsibilities for maintaining work force discipline in DOE. Chg 1, dated 3-11-85; Chg 2, dated 1-6-86; Chg 3, dated 3-21-89; Chg 4, dated 8-2-90; Chg 5, dated 3-9-92; Chg 6, dated 8-21-92, cancels Chg 5.

  15. Biology

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Biology @WIPP Life Begins at 250,000,000 Years WIPP's underground isn't just suited for ... radiation, one biology experiment actually conducts tests related to this phenomenon. ...

  16. The Biological Implications of the PP2A Crystal Structure

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    susceptibility to pathogen infections. To shed light on the details of how PP2A works, Cho and Xu have determined the first crystal structure of the enzyme. Their structure...

  17. Polarized Neutron in Structural Biology Present and Future Outlook

    SciTech Connect

    Zhao, Jinkui [ORNL; Robertson, Lee [ORNL; Herwig, Kenneth W [ORNL; Crabb, Don [University of Virginia

    2013-01-01

    Hydrogen has a strong polarization-dependent neutron scattering cross section. This property has been exploited in the study of soft matters, especially biological macromolecules. When a polarized neutron beam is scattered off a polarized hydrogenous sample, the otherwise large hydrogen incoherent cross section is drastically reduced while the coherent signal is significantly increased. Past experiments have demonstrated the potentials and benefits of polarized neutron scattering from soft materials. The main technical challenge of polarized neutron scattering from biological matters lies at sample polarization. Dynamic nuclear polarization is a proven yet rather sophisticated technique. Its complexity is one of the main reasons for the technique's slow adoption. The future of polarized neutron scattering in biology may rest largely in neutron protein crystallography. Polarization of protein crystals is much easier to accomplish, since protein crystals are typically rather small (<<1 mm) and only require small and easy- to-operate polarization apparatuses. In addition, the high resolution nature of neutron protein crystallography means that we will be able to study individual atoms using the polarized neutron scattering technique.

  18. Procedure for developing biological input for the design, location, or modification of water-intake structures

    SciTech Connect

    Neitzel, D.A.; McKenzie, D.H.

    1981-12-01

    To minimize adverse impact on aquatic ecosystems resulting from the operation of water intake structures, design engineers must have relevant information on the behavior, physiology and ecology of local fish and shellfish. Identification of stimulus/response relationships and the environmental factors that influence them is the first step in incorporating biological information in the design, location or modification of water intake structures. A procedure is presented in this document for providing biological input to engineers who are designing, locating or modifying a water intake structure. The authors discuss sources of stimuli at water intakes, historical approaches in assessing potential/actual impact and review biological information needed for intake design.

  19. The Biological Implications of the PP2A Crystal Structure

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Their structure provides a foundation for understanding PP2A regulation, satisfactory ... known as enzymes is a fundamental mechanism for the regulation of cellular activities. ...

  20. BioSAXS: Ways and Means to Study Structural Flexibility of Biological...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    BioSAXS: Ways and Means to Study Structural Flexibility of Biological Macromolecules Thursday, November 17, 2011 - 10:30am SSRL Conference Room 137-322 Dr. Alexander V. Shkumatov,...

  1. BioSAXS: Ways and Means to Study Structural Flexibility of Biological

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Macromolecules | Stanford Synchrotron Radiation Lightsource BioSAXS: Ways and Means to Study Structural Flexibility of Biological Macromolecules Thursday, November 17, 2011 - 10:30am SSRL Conference Room 137-322 Dr. Alexander V. Shkumatov, Biological Small Angle Scattering Group, EMBL Hamburg Small-angle X-ray scattering (SAXS) is universal low-resolution method to study native particles in solution and analyze structural changes in response to variations of external conditions. SAXS is

  2. STRUCTURAL BIOLOGY AND MOLECULAR MEDICINE RESEARCH PROGRAM (LSBMM)

    SciTech Connect

    Eisenberg, David S.

    2008-07-15

    The UCLA-DOE Institute of Genomics and Proteomics is an organized research unit of the University of California, sponsored by the Department of Energy through the mechanism of a Cooperative Agreement. Today the Institute consists of 10 Principal Investigators and 7 Associate Members, developing and applying technologies to promote the biological and environmental missions of the Department of Energy, and 5 Core Technology Centers to sustain this work. The focus is on understanding genomes, pathways and molecular machines in organisms of interest to DOE, with special emphasis on developing enabling technologies. Since it was founded in 1947, the UCLA-DOE Institute has adapted its mission to the research needs of DOE and its progenitor agencies as these research needs have changed. The Institute started as the AEC Laboratory of Nuclear Medicine, directed by Stafford Warren, who later became the founding Dean of the UCLA School of Medicine. In this sense, the entire UCLA medical center grew out of the precursor of our Institute. In 1963, the mission of the Institute was expanded into environmental studies by Director Ray Lunt. I became the third director in 1993, and in close consultation with David Galas and John Wooley of DOE, shifted the mission of the Institute towards genomics and proteomics. Since 1993, the Principal Investigators and Core Technology Centers are entirely new, and the Institute has separated from its former division concerned with PET imaging. The UCLA-DOE Institute shares the space of Boyer Hall with the Molecular Biology Institute, and assumes responsibility for the operation of the main core facilities. Fig. 1 gives the organizational chart of the Institute. Some of the benefits to the public of research carried out at the UCLA-DOE Institute include the following: The development of publicly accessible, web-based databases, including the Database of Protein Interactions, and the ProLinks database of genomicly inferred protein function linkages

  3. Biological Macromolecular Structures Data from the RCSB Protein Data Bank (RCSB PDB)

    DOE Data Explorer

    The Research Collaboratory for Structural Bioinformatics (RCSB) is a non-profit consortium that works to improve understanding of the function of biological systems through the study of the 3-D structure of biological macromolecules. The RCSB PDB is one of three sites serving as deposition, data processing, and distribution sites of the Protein Data Bank Archive. Each site provides its own view of the primary data, thus providing a variety of tools and resources for the global community. RCSB is also the official keeper for the PDB archive, with sole access authority to the PDB archive directory structure and contents. The RCSB PDB Information Portal for Biological Macromolecular Structures offers online tools for search and retrieval, for visualizing structures, for depositing, validating, or downloading data, news and highlights, a discussion forum, and links to other areas of related research. The PDB archive is a repository of atomic coordinates and other information describing proteins and other important biological macromolecules. Structural biologists use methods such as X-ray crystallography, NMR spectroscopy, and cryo-electron microscopy to determine the location of each atom relative to each other in the molecule. They then deposit this information, which is then annotated and publicly released into the archive by the wwPDB. Results can be viewed as 3-D images or models.

  4. DOE EPSCoR Initiative in Structural and computational Biology/Bioinformatics

    SciTech Connect

    Wallace, Susan S.

    2008-02-21

    The overall goal of the DOE EPSCoR Initiative in Structural and Computational Biology was to enhance the competiveness of Vermont research in these scientific areas. To develop self-sustaining infrastructure, we increased the critical mass of faculty, developed shared resources that made junior researchers more competitive for federal research grants, implemented programs to train graduate and undergraduate students who participated in these research areas and provided seed money for research projects. During the time period funded by this DOE initiative: (1) four new faculty were recruited to the University of Vermont using DOE resources, three in Computational Biology and one in Structural Biology; (2) technical support was provided for the Computational and Structural Biology facilities; (3) twenty-two graduate students were directly funded by fellowships; (4) fifteen undergraduate students were supported during the summer; and (5) twenty-eight pilot projects were supported. Taken together these dollars resulted in a plethora of published papers, many in high profile journals in the fields and directly impacted competitive extramural funding based on structural or computational biology resulting in 49 million dollars awarded in grants (Appendix I), a 600% return on investment by DOE, the State and University.

  5. WHOLE CELL TOMOGRAPHY/MOLECULAR BIOLOGY/STRUCTURAL BIOLOGY: Affordable x-ray microscopy with nanoscale resolution

    SciTech Connect

    Evans, James E.; Blackborow, Paul; Horne, Stephen J.; Gelb, Jeff

    2013-03-01

    Biological research spans 10 orders of magnitude from angstroms to meters. While electron microscopy can reveal structural details at most of these spatial length scales, transmission electron tomography only reliably reconstructs three-dimensional (3-D) volumes of cellular material with a spatial resolution between 1-5 nm from samples less than 500 nm thick1. Most biological cells are 2-30 times thicker than this threshold, which means that a cell must be cut into consecutive slices with each slice reconstructed individually in order to approximate the contextual information of the entire cell. Fortunately, due to a larger penetration depth2, X-ray computed tomography bypasses the need to physically section a cell and enables imaging of intact cells and tissues on the micrometer or larger scale with tens to hundreds of nanometer spatial resolution. While the technique of soft x-ray microscopy has been extensively developed in synchrotron facilities, advancements in laboratory x-ray source designs now increase its accessibility by supporting commercial systems suitable for a standard laboratory. In this paper, we highlight a new commercial compact cryogenic soft x-ray microscope designed for a standard laboratory setting and explore its capabilities for mesoscopic investigations of intact prokaryotic and eukaryotic cells.

  6. National Laboratory] Basic Biological Sciences(59) Biological...

    Office of Scientific and Technical Information (OSTI)

    Achievements of structural genomics Terwilliger, Thomas C. Los Alamos National Laboratory Basic Biological Sciences(59) Biological Science Biological Science Abstract Not...

  7. Data publication with the structural biology data grid supports live analysis

    DOE PAGES [OSTI]

    Meyer, Peter A.; Socias, Stephanie; Key, Jason; Ransey, Elizabeth; Tjon, Emily C.; Buschiazzo, Alejandro; Lei, Ming; Botka, Chris; Withrow, James; Neau, David; et al

    2016-03-07

    Access to experimental X-ray diffraction image data is fundamental for validation and reproduction of macromolecular models and indispensable for development of structural biology processing methods. Here, we established a diffraction data publication and dissemination system, Structural Biology Data Grid (SBDG; data.sbgrid.org), to preserve primary experimental data sets that support scientific publications. Data sets are accessible to researchers through a community driven data grid, which facilitates global data access. Our analysis of a pilot collection of crystallographic data sets demonstrates that the information archived by SBDG is sufficient to reprocess data to statistics that meet or exceed the quality of themore » original published structures. SBDG has extended its services to the entire community and is used to develop support for other types of biomedical data sets. In conclusion, it is anticipated that access to the experimental data sets will enhance the paradigm shift in the community towards a much more dynamic body of continuously improving data analysis.« less

  8. Cross-Linking and Mass Spectrometry Methodologies to Facilitate Structural Biology: Finding a Path through the Maze

    SciTech Connect

    Merkley, Eric D.; Cort, John R.; Adkins, Joshua N.

    2013-09-01

    Multiprotein complexes, rather than individual proteins, make up a large part of the biological macromolecular machinery of a cell. Understanding the structure and organization of these complexes is critical to understanding cellular function. Chemical cross-linking coupled with mass spectrometry is emerging as a complementary technique to traditional structural biology methods and can provide low-resolution structural information for a multitude of purposes, such as distance constraints in computational modeling of protein complexes. In this review, we discuss the experimental considerations for successful application of chemical cross-linking-mass spectrometry in biological studies and highlight three examples of such studies from the recent literature. These examples (as well as many others) illustrate the utility of a chemical cross-linking-mass spectrometry approach in facilitating structural analysis of large and challenging complexes.

  9. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    Sciences, Indiana University Schramm, Laurier L. (Laurier L. Schramm) - Chemistry ... Go back to Individual Researchers Collections: A B C D E F G H I J K L M N O P Q R S T U V ...

  10. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    ... for Artificial Intelligence Chinese Ceramic Society Chinese Chemical Society (CCS) Chinese Chemical Society (Taiwan) Chinese Environmental Mutagen Society (CEMS) Chinese ...

  11. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    ... (Arash Afraz) - McGovern Institute for Brain Research, Massachusetts Institute of Technology Afton, Alan D. (Alan D. Afton) - Louisiana Cooperative Fish and Wildlife Research Unit. ...

  12. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    University of Science and Technology Guzina, Bojan (Bojan Guzina) - Department of Civil Engineering, University of Minnesota Go back to Individual Researchers Collections: A B ...

  13. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    J K L M N O P Q R S T U V W X Y Z Joseph, Tim Grain (Tim Grain Joseph) - Department of Civil and Environmental Engineering, University of Alberta Go back to Individual Researchers ...

  14. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    A B C D E F G H I J K L M N O P Q R S T U V W X Y Z Colorado School of Mines, Department of Geophysics, Center for Rock Abuse Colorado School of Mines. Department of Geophysics, ...

  15. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    Q R S T U V W X Y Z Pennington, Wayne D.(Wayne D.Pennington).- Department of Geological Engineering and Sciences, Michigan Technological University Perloff, Jeffrey M. (Jeffrey M. ...

  16. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    F G H I J K L M N O P Q R S T U V W X Y Z Ferguson, Robert J. (Robert J. Ferguson) - Department of Geoscience, University of Calgary Firoozabadi, Abbas (Abbas Firoozabadi) - ...

  17. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    X Y Z Zevenhoven, Ron (Ron Zevenhoven) - Heat Engineering Laboratory, bo Akademi University, Go back to Individual Researchers Collections: A B C D E F G H I J K L M N O P Q R S ...

  18. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    I J K L M N O P Q R S T U V W X Y Z Ilic, Marija D. (Marija D. Ilic) - Department of Electrical and Computer Engineering, Carnegie Mellon University Go back to Individual ...

  19. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    M N O P Q R S T U V W X Y Z Li, Kewen (Kewen Li) - Geothermal Program, Department of Petroleum Engineering, Stanford University Lin, C.-Y. Cynthia (C.-Y. Cynthia Lin) - Departments ...

  20. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    N O P Q R S T U V W X Y Z Mallinson, Richard (Richard Mallinson) - School of Chemical Engineering and Materials Science, University of Oklahoma Mohaghegh, Shahab (Shahab Mohaghegh) ...

  1. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    V W X Y Z Ursin, Bjrn (Bjrn Ursin) - Department of Petroleum Engineering and Applied Geophysics, Norwegian University of Science and Technology Go back to Individual ...

  2. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    U V W X Y Z Texas at Austin, University of (University of Texas at Austin) - Bureau of Economic Geology, Exploration Geophysics Laboratory Torres-Verdn, Carlos (Carlos ...

  3. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    W X Y Z Valk, Peter (Peter Valk) - Department of Petroleum Engineering, Texas A&M University Go back to Individual Researchers Collections: A B C D E F G H I J K L M N O P Q R ...

  4. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    E F G H I J K L M N O P Q R S T U V W X Y Z Engelder, Terry (Terry Engelder) - Department of Geosciences, Pennsylvania State University Go back to Individual Researchers ...

  5. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    H I J K L M N O P Q R S T U V W X Y Z Haszeldine, Stuart (Stuart Haszeldine) - School of GeoSciences, University of Edinburgh Hubbard, Stephen Michial (Stephen Michial Hubbard) - ...

  6. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    Fossil Fuels Go to Research Groups Preprints Provided by Individual Scientists: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z Abu-Khamsin, Sidqi (Sidqi Abu-Khamsin) - ...

  7. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    S T U V W X Y Z Reynolds, Albert C. (Albert C. Reynolds) - Department of Petroleum Engineering, University of Tulsa Go back to Individual Researchers Collections: A B C D E F G H I ...

  8. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    E F G H I J K L M N O P Q R S T U V W X Y Z Dashtgard, Shahin (Shahin Dashtgard) - Department of Earth Sciences, Simon Fraser University Datta-Gupta, Akhil (Akhil Datta-Gupta) - ...

  9. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    Buckley, Jill S. (Jill S. Buckley) - Petroleum Recovery Research Center, New Mexico Tech Go back to Individual Researchers Collections: A B C D E F G H I J K L M N O P Q R S T ...

  10. Browse by Discipline -- E-print Network Subject Pathways: Geosciences...

    Office of Scientific and Technical Information (OSTI)

    C. Rau) - Laboratory of Physical and Structural Biology, National Institute of Child Health and Human Development Rauchfuss, Thomas B. (Thomas B. Rauchfuss) - Department of ...

  11. Structural effects of radiation-induced volumetric expansion on unreinforced concrete biological shields

    DOE PAGES [OSTI]

    Le Pape, Y.

    2015-11-22

    Limited literature (Pomaro et al., 2011, Mirhosseini et al., 2014, Salomoni et al., 2014 and Andreev and Kapliy, 2014) is available on the structural analysis of irradiated concrete biological shield (CBS), although extended operations of nuclear powers plants may lead to critical neutron exposure above 1.0 × 10+19 n cm₋2. To the notable exception of Andreev and Kapliy, available structural models do not account for radiation-induced volumetric expansion, although it was found to develop important linear dimensional change of the order of 1%, and, can lead to significant concrete damage (Le Pape et al., 2015). A 1D-cylindrical model of anmore » unreinforced CBS accounting for temperature and irradiation effects is developed. Irradiated concrete properties are characterized probabilistically using the updated database collected by Oak Ridge National Laboratory (Field et al., 2015). The overstressed concrete ratio (OCR) of the CBS, i.e., the proportion of the wall thickness being subject to stresses beyond the resistance of concrete, is derived by deterministic and probabilistic analysis assuming that irradiated concrete behaves as an elastic materials. In the bi-axial compressive zone near the reactor cavity, the OCR is limited to 5.7%, i.e., 8.6 cm (3$_2^1$ in.), whereas, in the tension zone, the OCR extends to 72%, i.e., 1.08 m (42$_2^1$ in.). Finally, we find that these results, valid for a maximum neutron fluence on the concrete surface of 3.1 × 10+19 n cm₋2 (E > 0.1 MeV) and, obtained after 80 years of operation, give an indication of the potential detrimental effects of prolonged irradiation of concrete in nuclear power plants.« less

  12. NEW - DOE O 333.1, Administering Work Force Discipline

    Directives, Delegations, and Other Requirements [Office of Management (MA)]

    The order provides requirements and responsibilities for administering work force discipline and corrective actions.

  13. 7th Annual Systems Biology Symposium: Systems Biology and Engineering

    SciTech Connect

    Galitski, Timothy P.

    2008-04-01

    Systems biology recognizes the complex multi-scale organization of biological systems, from molecules to ecosystems. The International Symposium on Systems Biology has been hosted by the Institute for Systems Biology in Seattle, Washington, since 2002. The annual two-day event gathers the most influential researchers transforming biology into an integrative discipline investingating complex systems. Engineering and application of new technology is a central element of systems biology. Genome-scale, or very small-scale, biological questions drive the enigneering of new technologies, which enable new modes of experimentation and computational analysis, leading to new biological insights and questions. Concepts and analytical methods in engineering are now finding direct applications in biology. Therefore, the 2008 Symposium, funded in partnership with the Department of Energy, featured global leaders in "Systems Biology and Engineering."

  14. How Clean is Safe? Improving the Effectiveness of Decontamination of Structures and People Following Chemical and Biological Incidents

    SciTech Connect

    Vogt , B.M.

    2003-04-03

    This report describes a U.S. Department of Energy, (DOE) Chemical and Biological National Security Program project that sought to establish what is known about decontamination of structures, objects, and people following an exposure to chemical or biological materials. Specifically we sought to identify the procedures and protocols used to determine when and how people or buildings are considered ''clean'' following decontamination. To fulfill this objective, the study systematically examined reported decontamination experiences to determine what procedures and protocols are currently employed for decontamination, the timeframe involved to initiate and complete the decontamination process, how the contaminants were identified, the factors determining when people were (or were not) decontaminated, the problems encountered during the decontamination process, how response efforts of agencies were coordinated, and the perceived social psychological effects on people who were decontaminated or who participated in the decontamination process. Findings and recommendations from the study are intended to aid decision-making and to improve the basis for determining appropriate decontamination protocols for recovery planners and policy makers for responding to chemical and biological events.

  15. The Protein Data Bank in Europe (PDBe): bringing structure to biology

    SciTech Connect

    Velankar, Sameer; Kleywegt, Gerard J.

    2011-04-01

    Some future challenges for the PDB and its guardians are discussed and current and future activities in structural bioinformatics at the Protein Data Bank in Europe (PDBe) are described. The Protein Data Bank in Europe (PDBe) is the European partner in the Worldwide PDB and as such handles depositions of X-ray, NMR and EM data and structure models. PDBe also provides advanced bioinformatics services based on data from the PDB and related resources. Some of the challenges facing the PDB and its guardians are discussed, as well as some of the areas on which PDBe activities will focus in the future (advanced services, ligands, integration, validation and experimental data). Finally, some recent developments at PDBe are described.

  16. FINAL REPORT: DOE CONTRACT NUMBER FG0205ER64026 Biological Neutron Scattering: A Collaboration with the Oak Ridge Center for Structural Molecular Biology

    SciTech Connect

    Jill Trewhella

    2011-01-12

    The overarching goal of this project was to promote applications of small-angle scattering in structural molecular biology by providing model examples of cutting edge applications that demonstrate the unique capabilities and potential of the DOE national user facilities at Oak Ridge, especially the newly commissioned BioSANS. The approach taken was three-fold: (1) to engage in high impact collaborative research projects that would benefit from small-angle neutron scattering to both demonstrate the power of the technique while expanding the potential user community; (2) to provide access to scattering facilities established at the University of Utah to as broad a set of researchers as possible to increase the expertise in small-angle scattering generally; and (3) to develop new methods and tools for small-angle scattering. To these ends, three major research collaborations were pursued that resulted in a significant body of published work where neutron scattering and contrast variation played a major role. These major collaborations involved studies of protein complexes involved in (1) bacterial transcription regulation and adaptive response (a DOE/BER priority area); (2) regulation of cardiac muscle; and (3) neuronal disorders. In addition, to broaden the impact of the project, smaller collaborative efforts were supported that used either small-angle X-ray or neutron scattering. Finally, the DOE supported facilities at the University of Utah were made available to researchers on a service basis and a number of independent groups took advantage of this opportunity. In all of this work, there was an emphasis on the training of students and post docs in scattering techniques, and a set of publications (a book chapter, a review, and an encyclopedia article) were produced to guide the non-specialist potential user of scattering techniques in successful applications of the techniques. We also developed a suite of user friendly web-based computational tools currently

  17. Crystallography Without Crystals: Determining the Structure of Individual Biological Molecules and Nanoparticles

    ScienceCinema

    Ourmazd, Abbas [University of Wisconsin, Milwaukee, Wisconsin, USA

    2016-07-12

    Ever shattered a valuable vase into 10 to the 6th power pieces and tried to reassemble it under a light providing a mean photon count of 10 minus 2 per detector pixel with shot noise? If you can do that, you can do single-molecule crystallography. This talk will outline how this can be done in principle. In more technical terms, the talk will describe how the combination of scattering physics and Bayesian algorithms can be used to reconstruct the 3-D diffracted intensity distribution from a collection of individual 2-D diffiraction patterns down to a mean photon count of 10 minus 2 per pixel, the signal level anticipated from the Linac Coherent Light Source, and hence determine the structure of individual macromolecules and nanoparticles.

  18. Structural studies of human Naked2: A biologically active intrinsically unstructured protein

    SciTech Connect

    Hu Tianhui; Krezel, Andrzej M.; Li Cunxi; Coffey, Robert J. . E-mail: robert.coffey@vanderbilt.edu

    2006-12-01

    Naked1 and 2 are two mammalian orthologs of Naked Cuticle, a canonical Wnt signaling antagonist in Drosophila. Naked2, but not Naked1, interacts with transforming growth factor-{alpha} (TGF{alpha}) and escorts TGF{alpha}-containing vesicles to the basolateral membrane of polarized epithelial cells. Full-length Naked2 is poorly soluble. Since most functional domains, including the Dishevelled binding region, EF-hand, vesicle recognition, and membrane targeting motifs, reside in the N-terminal half of the protein, we expressed and purified the first 217 residues of human Naked2 and performed a functional analysis of this fragment. Its circular dichroism (CD) and nuclear magnetic resonance (NMR) spectra showed no evidence of secondary and/or tertiary structure. The fragment did not bind calcium or zinc. These results indicate that the N-terminal half of Naked2 behaves as an intrinsically unstructured protein.

  19. Biologically Inspired Synthesis Route to Three-Dimensionally Structured Inorganic Thin Films

    DOE PAGES [OSTI]

    Schwenzer, Birgit; Morse, Daniel E.

    2008-01-01

    Inorganic thin films (hydroxide, oxide, and phosphate materials) that are textured on a submicron scale have been prepared from aqueous metal salt solutions at room temperature using vapor-diffusion catalysis. This generic synthesis approach mimics the essential advantages of the catalytic and structure-directing mechanisms observed for the formation of silica skeletons of marine sponges. Chemical composition, crystallinity, and the three-dimensional morphology of films prepared by this method are extremely sensitive to changes in the synthesis conditions, such as concentrations, reaction times, and the presence and nature of substrate materials. Focusing on different materials systems, the reaction mechanism for the formation ofmore » these thin films and the influence of different reaction parameters on the product are explained.« less

  20. A common-view disciplined oscillator

    SciTech Connect

    Lombardi, Michael A.; Dahlen, Aaron P.

    2010-05-15

    This paper describes a common-view disciplined oscillator (CVDO) that locks to a reference time scale through the use of common-view global positioning system (GPS) satellite measurements. The CVDO employs a proportional-integral-derivative controller that obtains near real-time common-view GPS measurements from the internet and provides steering corrections to a local oscillator. A CVDO can be locked to any time scale that makes real-time common-view data available and can serve as a high-accuracy, self-calibrating frequency and time standard. Measurement results are presented where a CVDO is locked to UTC(NIST), the coordinated universal time scale maintained at the National Institute of Standards and Technology in Boulder, Colorado.

  1. In situ X-ray data collection and structure phasing of protein crystals at Structural Biology Center 19-ID

    SciTech Connect

    Michalska, Karolina; Tan, Kemin; Chang, Changsoo; Li, Hui; Hatzos-Skintges, Catherine; Molitsky, Michael; Alkire, Randy; Joachimiak, Andrzej

    2015-10-15

    A prototype of a 96-well plate scanner forin situdata collection has been developed at the Structural Biology Center (SBC) beamline 19-ID, located at the Advanced Photon Source, USA. The applicability of this instrument for protein crystal diffraction screening and data collection at ambient temperature has been demonstrated. Several different protein crystals, including selenium-labeled, were used for data collection and successful SAD phasing. Without the common procedure of crystal handling and subsequent cryo-cooling for data collection atT= 100 K, crystals in a crystallization buffer show remarkably low mosaicity (<0.1°) until deterioration by radiation damage occurs. Data presented here show that cryo-cooling can cause some unexpected structural changes. Based on the results of this study, the integration of the plate scanner into the 19-ID end-station with automated controls is being prepared. With improvement of hardware and software,in situdata collection will become available for the SBC user program including remote access.

  2. DRAFT - DOE O 333.1, Administering Work Force Discipline

    Directives, Delegations, and Other Requirements [Office of Management (MA)]

    To provide requirements and responsibilities for administering workforce discipline that includes disciplinary, adverse, and alternative corrective actions in the Department of Energy (DOE).

  3. Michael Levitt and Computational Biology

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Biology Resources with Additional Information * Publications Michael Levitt Courtesy of Linda A. Cicero Stanford News Service Michael Levitt, PhD, professor of structural biology ...

  4. Biological Applications of Synchrotron Radiation:

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    A BioSync Report. Issued by the Structural Biology Synchrotron users Organization, ... of those beams has had a major impact on many areas of science, including biology. ...

  5. OLOI, Disciplined Operations - Good Practices Study in Organizational

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Change (OLOI 30-01, Rev. 0) | Department of Energy OLOI, Disciplined Operations - Good Practices Study in Organizational Change (OLOI 30-01, Rev. 0) OLOI, Disciplined Operations - Good Practices Study in Organizational Change (OLOI 30-01, Rev. 0) 10/18/2016 OLOI, Disciplined Operations - Good Practices Study in Organizational Change (OLOI 30-01, Rev. 0) One form of evaluation or analysis utilized by EA-30 is a study of an activity, event, or situation. The fundamental question of this study

  6. Browse by Discipline -- E-print Network Subject Pathways: Fossil...

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    (Thomas D. Schneider) - Laboratory of Experimental and Computational Biology, National Cancer Institute Scott, Matthew (Matthew Scott) - Departments of Developmental Biology, ...

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    of Organismic and Evolutionary Biology, Harvard University Hjek, Tom (Tom ... of Organismic and Evolutionary Biology, Harvard University Hartley, Stephen (Stephen ...

  8. Browse by Discipline -- E-print Network Subject Pathways: Plasma...

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    of Biological and Ecological Engineering, Oregon State University Semprini, Lewis (Lewis Semprini) - School of Chemical, Biological, and Environmental Engineering, Oregon ...

  9. Division of Biological and Medical Research research summary 1984-1985

    SciTech Connect

    Barr, S.H.

    1985-08-01

    The Division of Biological and Medical Research at Argonne National Laboratory conducts multidisciplinary research aimed at defining the biological and medical hazards to man from energy technologies and new energy options. These technically oriented studies have a strong base in fundamental research in a variety of scientific disciplines, including molecular and cellular biology, biophysics, genetics, radiobiology, pharmacology, biochemistry, chemistry, environmental toxicology, and epidemiology. This research summary is organized into six parts. The first five parts reflect the Divisional structure and contain the scientific program chapters, which summarize the activities of the individual groups during the calendar year 1984 and the first half of 1985. To provide better continuity and perspective, previous work is sometimes briefly described. Although the summaries are short, efforts have been made to indicate the range of research activities for each group.

  10. Physics Meets Biology (LBNL Summer Lecture Series)

    ScienceCinema

    Chu, Steve [Director, LBNL

    2016-07-12

    Summer Lecture Series 2006: If scientists could take advantage of the awesomely complex and beautiful functioning of biologys natural molecular machines, their potential for application in many disciplines would be incalculable. Nobel Laureate and Director of the Lawrence Berkeley National Laboratory Steve Chu explores Possible solutions to global warming and its consequences.

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    Xie, Xiaoliang Sunney (Xiaoliang Sunney Xie) - Department of Chemistry and Chemical Biology, Harvard University Xing, Bengang (Bengang Xing) - Division of Chemistry and Biological ...

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    Xie, Xiaoliang Sunney (Xiaoliang Sunney Xie) - Department of Chemistry and Chemical Biology, Harvard University Xing, Bengang (Bengang Xing) - Division of Chemistry and Biological ...

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    ... Gaudet, Rachelle (Rachelle Gaudet) - Department of Molecular and Cellular Biology, Harvard ... of Organismic and Evolutionary Biology, Harvard University Giri, Ranjit K. (Ranjit K. ...

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    ... Vitek, Christopher (Christopher Vitek) - Department of Biology, University of Texas - Pan America Vitkup, Dennis (Dennis Vitkup) - Center for Computational Biology and ...

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    ... Andrea (Andrea Marini) - Dipartimento di Fisica, Universit di Roma Tor Vergata ... Marko) - Departments of Biochemistry, Molecular Biology, and Cell Biology & Physics and ...

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    Department of Biology, University of New Mexico Nelson, Cara (Cara Nelson) - College of ... Nishiguchi) - Department of Biology, New Mexico State University Nishikawa, Kiisa (Kiisa ...

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  1. Genomics and Systems Biology

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    Genomics and Systems Biology Genomics and Systems Biology Los Alamos scientists perform research in functional genomics and structural genomics, and applications for such work cover diverse fields such as energy, agriculture, and environmental cleanup. Contact Us Babetta Marrone Biofuels Program Manager Email Cheryl Kuske DOE BER Biological System Science Division Program Manager Email Kirsten McCabe Emerging Threats Program Manager Email Rebecca McDonald Bioscience Communications Email "We

  2. Design of HIV-1 Protease Inhibitors with C3-Substituted Hexahydrocyclopentafuranyl Urethanes as P2-Ligands: Synthesis, Biological Evaluation, and Protein-Ligand X-ray Crystal Structure

    SciTech Connect

    Ghosh, Arun K; Chapsal, Bruno D; Parham, Garth L; Steffey, Melinda; Agniswamy, Johnson; Wang, Yuan-Fang; Amano, Masayuki; Weber, Irene T; Mitsuya, Hiroaki

    2011-11-07

    We report the design, synthesis, biological evaluation, and the X-ray crystal structure of a novel inhibitor bound to the HIV-1 protease. Various C3-functionalized cyclopentanyltetrahydrofurans (Cp-THF) were designed to interact with the flap Gly48 carbonyl or amide NH in the S2-subsite of the HIV-1 protease. We investigated the potential of those functionalized ligands in combination with hydroxyethylsulfonamide isosteres. Inhibitor 26 containing a 3-(R)-hydroxyl group on the Cp-THF core displayed the most potent enzyme inhibitory and antiviral activity. Our studies revealed a preference for the 3-(R)-configuration over the corresponding 3-(S)-derivative. Inhibitor 26 exhibited potent activity against a panel of multidrug-resistant HIV-1 variants. A high resolution X-ray structure of 26-bound HIV-1 protease revealed important molecular insight into the ligand-binding site interactions.

  3. Systems Biology in Prokaryote - Eukaryote Symbiosis | Stanford...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Systems Biology in Prokaryote - Eukaryote Symbiosis Monday, June 25, 2012 - 12:00pm SLAC, ... there are over 130 beamlines at synchrotrons worldwide devoted to structural biology. ...

  4. EIA: A splintering, exploding discipline with a massive new constituency

    SciTech Connect

    Johnson, Eric P.

    2015-02-15

    After serving 18 years as Editor-in-Chief of Environmental Impact Assessment Review, the author observes that the period 1997–2014, the discipline of EIA: splintered, exploded and saw the rise of the developing-world authors. Publishing has also changed, with shifts from quantity to quality, the rise of open access, and an ever-increasing shortage of reviewers.

  5. Penn State Multi-Discipline Tribology Group and Energy Institute Studies.

    SciTech Connect

    Perez, Joseph

    2001-08-05

    This presentation is a summary of the current research activities on fuels and lubricants in the Multi-discipline Tribology group and the engine test group in the Combustion Laboratory of the Pennsylvania State University. The progress areas discussed in this summary include those found in Table 1. Table 1. RESEARCH AREAS: Diesel Engine Emission Reduction; Oxygenated Fuels; Improved Friction Fuels; Vegetable Oil Lubricants; Extended Drain Lubricants; Effect of Chemical Structure on Friction and Wear. The research is of interest either directly or indirectly to the goal of this workshop, diesel engine emissions reduction. The current projects at Penn State in the areas listed above will be discussed.

  6. Structure of trigger factor binding domain in biologically homologous complex with eubacterial ribosome reveals its chaperone action

    SciTech Connect

    Baram, David; Pyetan, Erez; Sittner, Assa; Auerbach-Nevo, Tamar; Bashan, Anat; Yonath, Ada (WIS-I)

    2010-07-13

    Trigger factor (TF), the first chaperone in eubacteria to encounter the emerging nascent chain, binds to the large ribosomal subunit in the vicinity of the protein exit tunnel opening and forms a sheltered folding space. Here, we present the 3.5-{angstrom} crystal structure of the physiological complex of the large ribosomal subunit from the eubacterium Deinococcus radiodurans with the N-terminal domain of TF (TFa) from the same organism. For anchoring, TFa exploits a small ribosomal surface area in the vicinity of proteins L23 and L29, by using its 'signature motif' as well as additional structural elements. The molecular details of TFa interactions reveal that L23 is essential for the association of TF with the ribosome and may serve as a channel of communication with the nascent chain progressing in the tunnel. L29 appears to induce a conformational change in TFa, which results in the exposure of TFa hydrophobic patches to the opening of the ribosomal exit tunnel, thus increasing its affinity for hydrophobic segments of the emerging nascent polypeptide. This observation implies that, in addition to creating a protected folding space for the emerging nascent chain, TF association with the ribosome prevents aggregation by providing a competing hydrophobic environment and may be critical for attaining the functional conformation necessary for chaperone activity.

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    ... of Chemistry and Chemical Biology, Harvard University Lim, Sang-Hyun (Sang-Hyun Lim) ... of Chemistry and Chemical Biology, Harvard University Liu, Gang-yu (Gang-yu Liu) - ...

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    U V W X Y Z Ussery, David W. (David W. Ussery) - Center for Biological Sequence Analysis & Department of Systems Biology,Danmarks Tekniske Universitet Go back to Individual ...

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    and Computational Biology, National Cancer Institute Levitt, Michael (Michael ... (Xiaole Shirley Liu) - Dana-Farber Cancer Institute & Department of Biostatistics, ...

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    Goodrich) - Department of Neurobiology, Harvard University Gopalan, Venkat (Venkat ... - Department of Systems Biology, Harvard University Go back to Individual ...

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    University of Southern California Elofsson, Arne (Arne Elofsson) - Stockholm Bioinformatics Center, Stockholms Universitet Endy, Drew (Drew Endy) - Division of Biological ...

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    ... Department of Organismic and Evolutionary Biology, Harvard University Omiecinski, Curtis (Curtis Omiecinski) - Department of Veterinary and Biomedical Sciences, Pennsylvania State ...

  14. Biological Safety

    Office of Energy Efficiency and Renewable Energy (EERE)

    The DOE's Biological Safety Program provides a forum for the exchange of best practices, lessons learned, and guidance in the area of biological safety. This content is supported by the Biosurety Executive Team. The Biosurety Executive Team is a DOE-chartered group. The DOE Office of Worker Safety and Health Policy provides administrative support for this group. The group identifies biological safety-related issues of concern to the DOE and pursues solutions to issues identified.

  15. Biological Applications

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Biological Applications Biological Applications Bioscience technologies for biosecurity, infectious disease, and other threats. Bioscience Capabilities-for Collaboration View our capability sheets: get details of what we do. Atomic Magnetometer Micro Imaging MOL PCR Raman LIBS Waveguide Biosensor How we deploy innovation bioscience-foundational-science Cell biology hasn't been the same since Los Alamos pioneered flow cytometry in mid-1960s when a Lab scientist invented a cell separator that

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    ... Brigham and Women's Hospital, Harvard University de ... - Department of Biology, San Francisco State University ... de Magalhes, Joo Pedro (Joo Pedro de Magalhes) ...

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    of Chemistry, Ohio State University Dyson, Jane (Jane Dyson) - Department of Molecular Biology, Scripps Research Institute Go back to Individual Researchers Collections: A B C D ...

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    of South Carolina Alexandraki, Despina (Despina Alexandraki) - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas Altschuler, Steve ...

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    of Florida Tavernarakis, Nektarios (Nektarios Tavernarakis) - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas Tawfik, Dan S. ...

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    of South Carolina Alexandraki, Despina (Despina Alexandraki) - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas Altschuler, Steve ...

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    ... - School of Biology and Psychology, University of Newcastle upon Tyne Snell-Rood, Emilie C. (Emilie C. Snell-Rood) - Department of Ecology, Evolution, and Behavior, University of ...

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    ... Biology, Albert Einstein College of Medicine, Yeshiva University Snell-Rood, Emilie C. (Emilie C. Snell-Rood) - Department of Ecology, Evolution, and Behavior, University of ...

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    York University Roder, Heinrich (Heinrich Roder) - Basic Science Division, Fox Chase Cancer Center Rosenberg, Noah (Noah Rosenberg) - Department of Biology, Stanford University. ...

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    Biological Sciences, Virginia Tech Xiong, Yue (Yue Xiong) - Lineberger Comprehensive Cancer Center & Department of Biochemistry and Biophysics, University of North Carolina at ...

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    - Department of Environmental Science, Lancaster University Nakamura, Daisuke (Daisuke ... Biology, Harvard University Nielsen, Roger L. (Roger L. Nielsen) - College of Oceanic ...

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    of Engineering and Applied Sciences, Harvard University Martnez, Todd J. (Todd J. ... of Chemistry and Chemical Biology, Harvard University Mller, Peter (Peter Mller) ...

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    of Engineering and Applied Sciences, Harvard University Matin, A.C. (A.C. Matin) - ... of Organismic and Evolutionary Biology, Harvard University Morel, Franois M. M. ...

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    of Engineering and Applied Sciences, Harvard University Nagurka, Mark L. (Mark L. ... for Biologically Inspired Engineering, Harvard University Narain, Amitabh (Amitabh ...

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    Jackson, Sophie (Sophie Jackson) - Department of Chemistry, University of Cambridge Jacobsen, Eric N. (Eric N. Jacobsen) - Department of Chemistry and Chemical Biology, Harvard ...

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    University of California at Berkeley Caro, Tim (Tim Caro) - Department of Wildlife, Fish, and Conservation Biology, University of California, Davis Caron, David (David Caron) - ...

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    Ewen (Ewen Ritchie) - Department of Energy Technology, Aalborg University Rood, Stewart (Stewart Rood) - Department of Biological Sciences, University of Lethbridge Rosendahl, ...

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    Laaksonen, Toni (Toni Laaksonen) - Department of Biology, University of Turku Lastoskie, Christian M. (Christian M. Lastoskie) - Department of Civil and Environmental Engineering, ...

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    Biology of the Chloroplast US DOE, Office of Energy Efficiency and Renewable Energy, Green Power Network Utah, University of - Energy and Geoscience Institute, GeoSpatial ...

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    ... Vitek, Christopher (Christopher Vitek) - Department of Biology, University of Texas - Pan America Vittoz, Pascal (Pascal Vittoz) - Dpartement d'cologie et volution, ...

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    Y Z Xiao, John Q. (John Q. Xiao) - Department of Physics and Astronomy, University of Delaware Xing, Jianhua (Jianhua Xing) - Department of Biological Sciences, Virginia Tech Xu, ...

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    P. (Stephen P. Yanoviak) - Department of Biology, University of Arkansas at Little Rock Yeager, Kevin M. (Kevin M. Yeager) - Department of Marine Science, University of ...

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    Texas A&M University You, Lingchong (Lingchong You) - Department of Biomedical Engineering, Duke University Yu, Haiyuan (Haiyuan Yu) - Department of Biological ...

  8. Beamline 5.0.1

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    1 Print Berkeley Center for Structural Biology (BCSB) Monochromatic protein crystallography Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes...

  9. Beamline 5.0.2

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    Berkeley Center for Structural Biology (BCSB) Multiple-wavelength anomalous diffraction (MAD) and monochromatic protein crystallography Scientific discipline: Structural biology ...

  10. Beamline 5.0.1

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  11. Beamline 8.2.2

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  12. Beamline 8.2.1

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    Berkeley Center for Structural Biology (BCSB) Multiple-Wavelength Anomalous Diffraction (MAD) and Macromolecular Crystallography (MX) Scientific discipline: Structural biology ...

  13. Beamline 12.3.1

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    1 Print Structurally Integrated Biology for Life Sciences (SIBYLS) Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes Source characteristics...

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    Marine Biology, University of Hawai'i at Manoa Horowitz, Leah S. (Leah S. Horowitz) - Social Sciences Department, Hawai'i Pacific University Hung, I-Kuai (I-Kuai Hung) - College ...

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    S T U V W X Y Z Sayre, Richard (Richard Sayre) - Department of Plant Cellular and Molecular Biology, Ohio State University Schiff, Eric A. (Eric A. Schiff) - Department of Physics, ...

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    H I J K L M N O P Q R S T U V W X Y Z Garca, Oscar (Oscar Garca) - Forestry Program, University of Northern British Columbia Gieg, Lisa (Lisa Gieg) - Department of Biological...

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    Y Z Xi, Weimin (Weimin Xi) - Department of Biological and Health Sciences, Texas A&M University at Kingsville Xu, Kehui "Kevin" (Kehui "Kevin" Xu) - Department of Marine Science, ...

  18. Browse by Discipline -- E-print Network Subject Pathways: Environmenta...

    Office of Scientific and Technical Information (OSTI)

    Zhang, Y.-H. Percival (Y.-H. Percival Zhang) - Department of Biological Systems Engineering, Virginia Tech Go back to Individual Researchers Collections: A B C D E F G H I J K L M ...

  19. Comparison of 20 nm silver nanoparticles synthesized with and without a gold core. Structure, dissolution in cell culture media, and biological impact on macrophages

    SciTech Connect

    Munusamy, Prabhakaran; Wang, Chongmin; Engelhard, Mark H.; Baer, Donald R.; Smith, Jordan N.; Liu, Chongxuan; Kodali, Vamsi K.; Thrall, Brian D.; Chen, Shu; Porter, Alexandra E.; Ryan, Mary P.

    2015-07-15

    Widespread use of silver nanoparticles raises questions of environmental impact and toxicity. Both silver particles and silver ions formed by particle dissolution may impact biological systems. Therefore it is important to understand the characteristics of silver nanoparticles and their stability in relevant media. The synthesis route can impact physical and chemical characteristics of the particles and we report the characterization and solution stability of three types of silver nanoparticles (20 nm particles with and without gold cores and 110 nm particles with gold cores) in cell culture media with serum proteins: FBS10%/RPMI. These nanoparticles were synthesized in aqueous solution and characterized using both in situ and ex situ analysis methods. Dissolution studies were carried at particle concentrations from 1 µg/ml to 50 µg/ml. Particles with gold cores had smaller crystallite size and higher apparent solubility than pure silver particles. A dissolution model was found to describe the time variation of particle size and amount of dissolved silver for particle loadings above 9 µg/ml. An effective solubility product obtained from fitting the data was higher for the 20 nm gold core particles in comparison to the pure silver or 110 nm particles. Dissolution of the nanoparticles was enhanced by presence of serum proteins contained in fetal bovine serum. In addition, the protocol of the dispersion in the medium was found to influence particle agglomeration and dissolution. Results show that particle structure can impact the concentration of dissolved silver and the dose to which cells would be exposed during in vitro studies.

  20. Comparison of 20 nm silver nanoparticles synthesized with and without a gold core. Structure, dissolution in cell culture media, and biological impact on macrophages

    DOE PAGES [OSTI]

    Munusamy, Prabhakaran; Wang, Chongmin; Engelhard, Mark H.; Baer, Donald R.; Smith, Jordan N.; Liu, Chongxuan; Kodali, Vamsi K.; Thrall, Brian D.; Chen, Shu; Porter, Alexandra E.; et al

    2015-07-15

    Widespread use of silver nanoparticles raises questions of environmental impact and toxicity. Both silver particles and silver ions formed by particle dissolution may impact biological systems. Therefore it is important to understand the characteristics of silver nanoparticles and their stability in relevant media. The synthesis route can impact physical and chemical characteristics of the particles and we report the characterization and solution stability of three types of silver nanoparticles (20 nm particles with and without gold cores and 110 nm particles with gold cores) in cell culture media with serum proteins: FBS10%/RPMI. These nanoparticles were synthesized in aqueous solution andmore » characterized using both in situ and ex situ analysis methods. Dissolution studies were carried at particle concentrations from 1 µg/ml to 50 µg/ml. Particles with gold cores had smaller crystallite size and higher apparent solubility than pure silver particles. A dissolution model was found to describe the time variation of particle size and amount of dissolved silver for particle loadings above 9 µg/ml. An effective solubility product obtained from fitting the data was higher for the 20 nm gold core particles in comparison to the pure silver or 110 nm particles. Dissolution of the nanoparticles was enhanced by presence of serum proteins contained in fetal bovine serum. In addition, the protocol of the dispersion in the medium was found to influence particle agglomeration and dissolution. Results show that particle structure can impact the concentration of dissolved silver and the dose to which cells would be exposed during in vitro studies.« less

  1. Biological preconcentrator

    DOEpatents

    Manginell, Ronald P.; Bunker, Bruce C.; Huber, Dale L.

    2008-09-09

    A biological preconcentrator comprises a stimulus-responsive active film on a stimulus-producing microfabricated platform. The active film can comprise a thermally switchable polymer film that can be used to selectively absorb and desorb proteins from a protein mixture. The biological microfabricated platform can comprise a thin membrane suspended on a substrate with an integral resistive heater and/or thermoelectric cooler for thermal switching of the active polymer film disposed on the membrane. The active polymer film can comprise hydrogel-like polymers, such as poly(ethylene oxide) or poly(n-isopropylacrylamide), that are tethered to the membrane. The biological preconcentrator can be fabricated with semiconductor materials and technologies.

  2. (Biological dosimetry)

    SciTech Connect

    Sega, G.A.

    1990-11-06

    The traveler participated in an International Symposium on Trends in Biological Dosimetry and presented an invited paper entitled, Adducts in sperm protamine and DNA vs mutation frequency.'' The purpose of the Symposium was to examine the applicability of new methods to study quantitatively the effects of xenobiotic agents (radiation and chemicals) on molecular, cellular and organ systems, with special emphasis on human biological dosimetry. The general areas covered at the meeting included studies on parent compounds and metabolites; protein adducts; DNA adducts; gene mutations; cytogenetic end-points and reproductive methods.

  3. Theoretical Biology and Biophysics

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Theoretical Biology and Biophysics Modeling biological systems and analysis and informatics of molecular and cellular biological data Mathematical BiologyImmunology Fundamental ...

  4. (Biological dosimetry)

    SciTech Connect

    Preston, R.J.

    1990-12-17

    The traveler attended the 1st International Conference on Biological Dosimetry in Madrid, Spain. This conference was organized to provide information to a general audience of biologists, physicists, radiotherapists, industrial hygiene personnel and individuals from related fields on the current ability of cytogenetic analysis to provide estimates of radiation dose in cases of occupational or environmental exposure. There is a growing interest in Spain in biological dosimetry because of the increased use of radiation sources for medical and occupational uses, and with this the anticipated and actual increase in numbers of overexposure. The traveler delivered the introductory lecture on Biological Dosimetry: Mechanistic Concepts'' that was intended to provide a framework by which the more applied lectures could be interpreted in a mechanistic way. A second component of the trip was to provide advice with regard to several recent cases of overexposure that had been or were being assessed by the Radiopathology and Radiotherapy Department of the Hospital General Gregorio Maranon'' in Madrid. The traveler had provided information on several of these, and had analyzed cells from some exposed or purportedly exposed individuals. The members of the biological dosimetry group were referred to individuals at REACTS at Oak Ridge Associated Universities for advice on follow-up treatment.

  5. Browse by Discipline -- E-print Network Subject Pathways: Fossil...

    Office of Scientific and Technical Information (OSTI)

    B C D E F G H I J K L M N O P Q R S T U V W X Y Z Babu, M. Madan (M. Madan Babu) - Laboratory of Molecular Biology, MRC Badger, Jonathan (Jonathan Badger) - Institute for Genomic ...

  6. Browse by Discipline -- E-print Network Subject Pathways: Power...

    Office of Scientific and Technical Information (OSTI)

    ... A B C D E F G H I J K L M N O P Q R S T U V W X Y Z Eawag Limnological Research Centre, Department Fish Ecology and Evolution Edinburgh, University of - School of Biological ...

  7. Browse by Discipline -- E-print Network Subject Pathways: Environmenta...

    Office of Scientific and Technical Information (OSTI)

    R S T U V W X Y Z Qin, Wensheng (Wensheng Qin) - Department of Biology, Lakehead University Go back to Individual Researchers Collections: A B C D E F G H I J K L M N O P Q R S T U...

  8. Browse by Discipline -- E-print Network Subject Pathways: --...

    Office of Scientific and Technical Information (OSTI)

    ... University Pereira, Henrique Miguel (Henrique Miguel Pereira) - Centro de Biologia Ambiental, Universidade de Lisboa Perera, Lalith (Lalith Perera) - Laboratory of Structural ...

  9. Browse by Discipline -- E-print Network Subject Pathways: Chemistry...

    Office of Scientific and Technical Information (OSTI)

    Johnson) - Department of Physics and Astronomy, University of Oklahoma Jonas, Ulrich (Ulrich Jonas) - Institute of Electronic Structure and Laser, Foundation for Research and ...

  10. Browse by Discipline -- E-print Network Subject Pathways: Chemistry...

    Office of Scientific and Technical Information (OSTI)

    Kafesaki, Maria (Maria Kafesaki) - Institute of Electronic Structure and Laser, Foundation ... Charles (Charles Kane) - Department of Physics and Astronomy, University of Pennsylvania ...

  11. BEAMLINE 2-3

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    imaging MAIN SCIENTIFIC DISCIPLINES: Biology Materials Chemistry Environmental % ... Sample Preparation Labs: The SSRL Structural Molecular Biology (SMB) Sample Preparation ...

  12. BEAMLINE 11-3 Materials Diffraction

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    DISCIPLINES: Materials Environmental Biology % TIME GENERAL USE: 100% SCHEDULING: ... Sample Preparation Labs: The SSRL Structural Molecular Biology (SMB) Sample Preparation ...

  13. BEAMLINE 10-2

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    scattering MAIN SCIENTIFIC DISCIPLINES: Biology Materials Environmental % TIME ... Labs: The SSRL Structural Molecular Biology (SMB) Sample Preparation Laboratories are ...

  14. Beamline 4.2.2

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Molecular Biology Consortium Multiple-Wavelength Anomalous Diffraction (MAD) and Macromolecular Crystallography (MX) Scientific discipline: Structural biology GENERAL BEAMLINE ...

  15. Multi-discipline Waste Acceptance Process at the Nevada National Security Site - 13573

    SciTech Connect

    Carilli, Jhon T.; Krenzien, Susan K.

    2013-07-01

    The Nevada National Security Site low-level radioactive waste disposal facility acceptance process requires multiple disciplines to ensure the protection of workers, the public, and the environment. These disciplines, which include waste acceptance, nuclear criticality, safety, permitting, operations, and performance assessment, combine into the overall waste acceptance process to assess low-level radioactive waste streams for disposal at the Area 5 Radioactive Waste Management Site. Four waste streams recently highlighted the integration of these disciplines: the Oak Ridge Radioisotope Thermoelectric Generators and Consolidated Edison Uranium Solidification Project material, West Valley Melter, and classified waste. (authors)

  16. Genomics and Systems Biology

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Genomics and Systems Biology Los Alamos scientists perform research in functional genomics ... and experimental biology as the foundation of a dynamic systems biology capability. ...

  17. Frontiers of NMR in Molecular Biology

    SciTech Connect

    1999-08-25

    NMR spectroscopy is expanding the horizons of structural biology by determining the structures and describing the dynamics of blobular proteins in aqueous solution, as well as other classes of proteins including membrane proteins and the polypeptides that form the aggregates diagnostic of prion and amyloid diseases. Significant results are also emerging on DNA and RNA oligomers and their complexes with proteins. This meeting focused attention on key structural questions emanating from molecular biology and how NMR spectroscopy can be used to answer them.

  18. Neutron instrumentation for biology

    SciTech Connect

    Mason, S.A.

    1994-12-31

    In the October 1994 round of proposals at the ILL, the external biology review sub- committee was asked to allocate neutron beam time to a wide range of experiments, on almost half the total number of scheduled neutron instruments: on 3 diffractometers, on 3 small angle scattering instruments, and on some 6 inelastic scattering spectrometers. In the 3.5 years since the temporary reactor shutdown, the ILL`s management structure has been optimized, budgets and staff have been trimmed, the ILL reactor has been re-built, and many of the instruments up-graded, many powerful (mainly Unix) workstations have been introduced, and the neighboring European Synchrotron Radiation Facility has established itself as the leading synchrotron radiation source and has started its official user program. The ILL reactor remains the world`s most intense dedicated neutron source. In this challenging context, it is of interest to review briefly the park of ILL instruments used to study the structure and energetics of small and large biological systems. A brief summary will be made of each class of experiments actually proposed in the latest ILL proposal round.

  19. S09 Symposium KK, Structure-Property Relationships in Biomineralized and Biomimetic Composites

    SciTech Connect

    David Kisailus; Lara Estroff; Himadri S. Gupta; William J. Landis; Pablo D. Zavattieri

    2010-06-07

    The technical presentations and discussions at this symposium disseminated and assessed current research and defined future directions in biomaterials research, with a focus on structure-function relationships in biological and biomimetic composites. The invited and contributed talks covered a diverse range of topics from fundamental biology, physics, chemistry, and materials science to potential applications in developing areas such as light-weight composites, multifunctional and smart materials, biomedical engineering, and nanoscaled sensors. The invited speakers were chosen to create a stimulating program with a mixture of established and junior faculty, industrial and academic researchers, and American and international experts in the field. This symposium served as an excellent introduction to the area for younger scientists (graduate students and post-doctoral researchers). Direct interactions between participants also helped to promote potential future collaborations involving multiple disciplines and institutions.

  20. Report of the Structural Biology Subcommittee of the Biological...

    Office of Science (SC)

    ... David Kingsbury (absent from meeting) Chiron Corporation Emeryville, California Dr. Anthony Kossiakoff University of Chicago Chicago, Illinois Dr. Janet Smith Purdue University ...

  1. 6th Annual Systems Biology Symposium: Systems Biology and the Environment

    SciTech Connect

    Galitski, Timothy, P.

    2007-04-01

    Systems biology recognizes the complex multi-scale organization of biological systems, from molecules to ecosystems. The International Symposium on Systems Biology is an annual two-day event gathering the most influential researchers transforming biology into an integrative discipline investigating complex systems. In recognition of the fundamental similarity between the scientific problems addressed in environmental science and systems biology studies at the molecular, cellular, and organismal levels, the 2007 Symposium featured global leaders in “Systems Biology and the Environment.” The objective of the 2007 “Systems Biology and the Environment” International Symposium was to stimulate interdisciplinary thinking and research that spans systems biology and environmental science. This Symposium was well aligned with the DOE’s Genomics:GTL program efforts to achieve scientific objectives for each of the three DOE missions: • Develop biofuels as a major secure energy source for this century, • Develop biological solutions for intractable environmental problems, and • Understand biosystems’ climate impacts and assess sequestration strategies Our scientific program highlighted world-class research exemplifying these priorities. The Symposium featured 45 minute lectures from 12 researchers including: Penny/Sallie Chisholm of MIT gave the keynote address “Tiny Cells, Global Impact: What Prochlorococcus Can Teach Us About Systems Biology”, plus Jim Fredrickson of PNNL, Nitin Baliga of ISB, Steve Briggs of UCSD, David Cox of Perlegen Sciences, Antoine Danchin of Institut Pasteur, John Delaney of the U of Washington, John Groopman of Johns Hopkins, Ben Kerr of the U of Washington, Steve Koonin of BP, Elliott Meyerowitz of Caltech, and Ed Rubin of LBNL. The 2007 Symposium promoted DOE’s three mission areas among scientists from multiple disciplines representing academia, non-profit research institutions, and the private sector. As in all previous

  2. Work with Biological Materials

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    cells, viruses), plant or soil samples (USDA quarantines), recombinant DNA, or blood-borne pathogen. Biological Use Authorization The great majority of biological work at...

  3. Biological conversion system

    DOEpatents

    Scott, C.D.

    A system for bioconversion of organic material comprises a primary bioreactor column wherein a biological active agent (zymomonas mobilis) converts the organic material (sugar) to a product (alcohol), a rejuvenator column wherein the biological activity of said biological active agent is enhanced, and means for circulating said biological active agent between said primary bioreactor column and said rejuvenator column.

  4. Ninth International Workshop on Plant Membrane Biology

    SciTech Connect

    Not Available

    1993-12-31

    This report is a compilation of abstracts from papers which were discussed at a workshop on plant membrane biology. Topics include: plasma membrane ATP-ases; plant-environment interactions, membrane receptors; signal transduction; ion channel physiology; biophysics and molecular biology; vaculor H+ pumps; sugar carriers; membrane transport; and cellular structure and function.

  5. Systems biology approach to bioremediation

    SciTech Connect

    Chakraborty, Romy; Wu, Cindy H.; Hazen, Terry C.

    2012-06-01

    Bioremediation has historically been approached as a ‘black box’ in terms of our fundamental understanding. Thus it succeeds and fails, seldom without a complete understanding of why. Systems biology is an integrated research approach to study complex biological systems, by investigating interactions and networks at the molecular, cellular, community, and ecosystem level. The knowledge of these interactions within individual components is fundamental to understanding the dynamics of the ecosystem under investigation. Finally, understanding and modeling functional microbial community structure and stress responses in environments at all levels have tremendous implications for our fundamental understanding of hydrobiogeochemical processes and the potential for making bioremediation breakthroughs and illuminating the ‘black box’.

  6. Plant Vascular Biology 2010

    SciTech Connect

    Ding, Biao

    2014-11-17

    This grant supported the Second International Conference on Plant Vascular Biology (PVB 2010) held July 24-28, 2010 on the campus of Ohio State University, Columbus, Ohio. Biao Ding (Ohio State University; OSU) and David Hannapel (Iowa State University; ISU) served as co-chairs of this conference. Biao Ding served as the local organizer. PVB is defined broadly here to include studies on the biogenesis, structure and function of transport systems in plants, under conditions of normal plant growth and development as well as of plant interactions with pathogens. The transport systems cover broadly the xylem, phloem, plasmodesmata and vascular cell membranes. The PVB concept has emerged in recent years to emphasize the integrative nature of the transport systems and approaches to investigate them.

  7. The Impact of Structural Genomics: Expectations and Outcomes...

    Office of Scientific and Technical Information (OSTI)

    solved by SG centers, and contrast these results with traditional structural biology. ... However, top structural biology laboratories are much more efficient than the average, and ...

  8. Micro/nanofabricated environments for synthetic biology (Journal...

    Office of Scientific and Technical Information (OSTI)

    Artificial micro- and nanofabricated structures have helped elucidate the effects of nanoscale spatial confinement and segregation on biological behavior, particularly when ...

  9. Beamline 5.0.3

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    0.3 Print Berkeley Center for Structural Biology (BCSB) Monochromatic protein crystallography Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes...

  10. Beamline 5.0.3

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Beamline 5.0.3 Print Berkeley Center for Structural Biology (BCSB) Monochromatic protein crystallography Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION...

  11. BEAMLINE 7-3

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    MAIN SCIENTIFIC DISCIPLINES: Structural Biology % TIME GENERAL USE: 100% SCHEDULING: ... Labs: The SSRL Structural Molecular Biology (SMB) Sample Preparation Laboratories are ...

  12. Beamline 5.0.3

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    0.3 Print Berkeley Center for Structural Biology (BCSB) Monochromatic protein crystallography Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes ...

  13. Beamline 5.0.3

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Beamline 5.0.3 Print Berkeley Center for Structural Biology (BCSB) Monochromatic protein crystallography Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION ...

  14. Beamline 12.3.1

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    3.1 Print Structurally Integrated Biology for Life Sciences (SIBYLS) Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes Source characteristics...

  15. Beamline 12.3.1

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Beamline 12.3.1 Print Tuesday, 20 October 2009 09:33 Structurally Integrated Biology for Life Sciences (SIBYLS) Scientific discipline: Structural biology GENERAL BEAMLINE...

  16. Beamline 12.3.1

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    12.3.1 Print Structurally Integrated Biology for Life Sciences (SIBYLS) Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes Source...

  17. Beamline 5.0.1

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Beamline 5.0.1 Print Berkeley Center for Structural Biology (BCSB) Monochromatic protein crystallography Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION ...

  18. Electronic structure of superconductivity refined

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Electronic structure of superconductivity refined Electronic structure of superconductivity refined A team of physicists propose a new model that expands on a little understood aspect of the electronic structure in high-temperature superconductors. July 10, 2008 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy sources, to plasma

  19. Beamline 8.3.1

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    1 Print Multiple-wavelength anomalous diffraction (MAD) and macromolecular crystallography (MX) Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational...

  20. Advanced Photon Source (APS) | U.S. DOE Office of Science (SC...

    Office of Science (SC)

    in a variety of disciplines, including condensed matter physics, materials sciences, chemistry, geosciences, structural biology, medical imaging, and environmental sciences. ...

  1. Biological tracer method

    DOEpatents

    Strong-Gunderson, J.M.; Palumbo, A.V.

    1998-09-15

    The present invention is a biological tracer method for characterizing the movement of a material through a medium, comprising the steps of: introducing a biological tracer comprising a microorganism having ice nucleating activity into a medium; collecting at least one sample of the medium from a point removed from the introduction point; and analyzing the sample for the presence of the biological tracer. The present invention is also a method for using a biological tracer as a label for material identification by introducing a biological tracer having ice nucleating activity into a material, collecting a sample of a portion of the labelled material and analyzing the sample for the presence of the biological tracer. 2 figs.

  2. Biological tracer method

    DOEpatents

    Strong-Gunderson, Janet M. (Ten Mile, TN); Palumbo, Anthony V. (Oak Ridge, TN)

    1998-01-01

    The present invention is a biological tracer method for characterizing the movement of a material through a medium, comprising the steps of: introducing a biological tracer comprising a microorganism having ice nucleating activity into a medium; collecting at least one sample of the medium from a point removed from the introduction point; and analyzing the sample for the presence of the biological tracer. The present invention is also a method for using a biological tracer as a label for material identification by introducing a biological tracer having ice nucleating activity into a material, collecting a sample of a portion of the labelled material and analyzing the sample for the presence of the biological tracer.

  3. Biological detector and method

    DOEpatents

    Sillerud, Laurel; Alam, Todd M.; McDowell, Andrew F.

    2015-11-24

    A biological detector includes a conduit for receiving a fluid containing one or more magnetic nanoparticle-labeled, biological objects to be detected and one or more permanent magnets or electromagnet for establishing a low magnetic field in which the conduit is disposed. A microcoil is disposed proximate the conduit for energization at a frequency that permits detection by NMR spectroscopy of whether the one or more magnetically-labeled biological objects is/are present in the fluid.

  4. Biological detector and method

    DOEpatents

    Sillerud, Laurel; Alam, Todd M; McDowell, Andrew F

    2013-02-26

    A biological detector includes a conduit for receiving a fluid containing one or more magnetic nanoparticle-labeled, biological objects to be detected and one or more permanent magnets or electromagnet for establishing a low magnetic field in which the conduit is disposed. A microcoil is disposed proximate the conduit for energization at a frequency that permits detection by NMR spectroscopy of whether the one or more magnetically-labeled biological objects is/are present in the fluid.

  5. Biological detector and method

    DOEpatents

    Sillerud, Laurel; Alam, Todd M; McDowell, Andrew F

    2014-04-15

    A biological detector includes a conduit for receiving a fluid containing one or more magnetic nanoparticle-labeled, biological objects to be detected and one or more permanent magnets or electromagnet for establishing a low magnetic field in which the conduit is disposed. A microcoil is disposed proximate the conduit for energization at a frequency that permits detection by NMR spectroscopy of whether the one or more magnetically-labeled biological objects is/are present in the fluid.

  6. 2010 Plant Molecular Biology Gordon Research Conference

    SciTech Connect

    Michael Sussman

    2010-07-23

    The Plant Molecular Biology Conference has traditionally covered a breadth of exciting topics and the 2010 conference will continue in that tradition. Emerging concerns about food security have inspired a program with three main themes: (1) genomics, natural variation and breeding to understand adaptation and crop improvement, (2) hormonal cross talk, and (3) plant/microbe interactions. There are also sessions on epigenetics and proteomics/metabolomics. Thus this conference will bring together a range of disciplines, will foster the exchange of ideas and enable participants to learn of the latest developments and ideas in diverse areas of plant biology. The conference provides an excellent opportunity for individuals to discuss their research because additional speakers in each session will be selected from submitted abstracts. There will also be a poster session each day for a two-hour period prior to dinner. In particular, this conference plays a key role in enabling students and postdocs (the next generation of research leaders) to mingle with pioneers in multiple areas of plant science.

  7. Genomics and Systems Biology

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    This approach promises to enhance all aspects of biological research such as: * Increasing the specificity of pathogen detection * Designing more effective drugs through ...

  8. Molecular Mechanism of Biological Proton Transport

    SciTech Connect

    Pomes, R.

    1998-09-01

    Proton transport across lipid membranes is a fundamental aspect of biological energy transduction (metabolism). This function is mediated by a Grotthuss mechanism involving proton hopping along hydrogen-bonded networks embedded in membrane-spanning proteins. Using molecular simulations, the authors have explored the structural, dynamic, and thermodynamic properties giving rise to long-range proton translocation in hydrogen-bonded networks involving water molecules, or water wires, which are emerging as ubiquitous H{sup +}-transport devices in biological systems.

  9. Renewable Hydrogen Production from Biological Systems

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Hydrogen Production from Biological Systems Matthew Posewitz Colorado School of Mines DOE Biological Hydrogen Production Workshop September 24 th , 2013 H 2 production PSII/PSI pathway PSI/nonphotochemical PQ Dark fermentation H 2 uptake oxyhydrogen reaction photoreduction Photosynthetic H 2 pathways Peters JW et al. Science 1998 Nicolet Y et al. Structure and Folding Des. 1999 Phototroph Hydrogenases * Cyanobacteria - Only [NiFe]-hydrogenases identified to date. - Typically dark H 2 production.

  10. Micro/nanofabricated environments for synthetic biology

    SciTech Connect

    Collier, Pat [ORNL; Simpson, Michael L [ORNL

    2011-01-01

    A better understanding of how confinement, crowding and reduced dimensionality modulate reactivity and reaction dynamics will aid in the rational and systematic discovery of functionality in complex biological systems. Artificial micro- and nanofabricated structures have helped elucidate the effects of nanoscale spatial confinement and segregation on biological behavior, particularly when integrated with microfluidics, through precise control in both space and time of diffusible signals and binding interactions. Examples of nanostructured interfaces for synthetic biology include the development of cell-like compartments for encapsulating biochemical reactions, nanostructured environments for fundamental studies of diffusion, molecular transport and biochemical reaction kinetics, and regulation of biomolecular interactions as functions of micro- and nanofabricated topological constraints.

  11. Integrated Biological Control

    SciTech Connect

    JOHNSON, A.R.

    2002-09-01

    Biological control is any activity taken to prevent, limit, clean up, or remediate potential environmental, health and safety, or workplace quality impacts from plants, animals, or microorganisms. At Hanford the principal emphasis of biological control is to prevent the transport of radioactive contamination by biological vectors (plants, animals, or microorganisms), and where necessary, control and clean up resulting contamination. Other aspects of biological control at Hanford include industrial weed control (e.g.; tumbleweeds), noxious weed control (invasive, non-native plant species), and pest control (undesirable animals such as rodents and stinging insects; and microorganisms such as molds that adversely affect the quality of the workplace environment). Biological control activities may be either preventive (apriori) or in response to existing contamination spread (aposteriori). Surveillance activities, including ground, vegetation, flying insect, and other surveys, and apriori control actions, such as herbicide spraying and placing biological barriers, are important in preventing radioactive contamination spread. If surveillance discovers that biological vectors have spread radioactive contamination, aposteriori control measures, such as fixing contamination, followed by cleanup and removal of the contamination to an approved disposal location are typical response functions. In some cases remediation following the contamination cleanup and removal is necessary. Biological control activities for industrial weeds, noxious weeds and pests have similar modes of prevention and response.

  12. Integrated Biological Control

    SciTech Connect

    JOHNSON, A.R.

    2003-10-09

    Biological control is any activity taken to prevent, limit, clean up, or remediate potential environmental, health and safety, or workplace quality impacts from plants, animals, or microorganisms. At Hanford the principal emphasis of biological control is to prevent the transport of radioactive contamination by biological vectors (plants, animals, or microorganisms), and where necessary, control and clean up resulting contamination. Other aspects of biological control at Hanford include industrial weed control (e.g.; tumbleweeds), noxious weed control (invasive, non-native plant species), and pest control (undesirable animals such as rodents and stinging insects, and microorganisms such as molds that adversely affect the quality of the workplace environment). Biological control activities may be either preventive (a priori) or in response to existing contamination spread (a posteriori). Surveillance activities, including ground, vegetation, flying insect, and other surveys, and a priori control actions, such as herbicide spraying and placing biological barriers, are important in preventing radioactive contamination spread. If surveillance discovers that biological vectors have spread radioactive contamination, a posteriori control measures, such as fixing contamination, followed by cleanup and removal of the contamination to an approved disposal location are typical response functions. In some cases remediation following the contamination cleanup and removal is necessary. Biological control activities for industrial weeds, noxious weeds and pests have similar modes of prevention and response.

  13. Biological sample collector

    DOEpatents

    Murphy, Gloria A.

    2010-09-07

    A biological sample collector is adapted to a collect several biological samples in a plurality of filter wells. A biological sample collector may comprise a manifold plate for mounting a filter plate thereon, the filter plate having a plurality of filter wells therein; a hollow slider for engaging and positioning a tube that slides therethrough; and a slide case within which the hollow slider travels to allow the tube to be aligned with a selected filter well of the plurality of filter wells, wherein when the tube is aligned with the selected filter well, the tube is pushed through the hollow slider and into the selected filter well to sealingly engage the selected filter well and to allow the tube to deposit a biological sample onto a filter in the bottom of the selected filter well. The biological sample collector may be portable.

  14. Semiconductor Nanocrystals for Biological Imaging

    SciTech Connect

    Fu, Aihua; Gu, Weiwei; Larabell, Carolyn; Alivisatos, A. Paul

    2005-06-28

    Conventional organic fluorophores suffer from poor photo stability, narrow absorption spectra and broad emission feature. Semiconductor nanocrystals, on the other hand, are highly photo-stable with broad absorption spectra and narrow size-tunable emission spectra. Recent advances in the synthesis of these materials have resulted in bright, sensitive, extremely photo-stable and biocompatible semiconductor fluorophores. Commercial availability facilitates their application in a variety of unprecedented biological experiments, including multiplexed cellular imaging, long-term in vitro and in vivo labeling, deep tissue structure mapping and single particle investigation of dynamic cellular processes. Semiconductor nanocrystals are one of the first examples of nanotechnology enabling a new class of biomedical applications.

  15. Biological and Chemical Security

    SciTech Connect

    Fitch, P J

    2002-12-19

    The LLNL Chemical & Biological National Security Program (CBNP) provides science, technology and integrated systems for chemical and biological security. Our approach is to develop and field advanced strategies that dramatically improve the nation's capabilities to prevent, prepare for, detect, and respond to terrorist use of chemical or biological weapons. Recent events show the importance of civilian defense against terrorism. The 1995 nerve gas attack in Tokyo's subway served to catalyze and focus the early LLNL program on civilian counter terrorism. In the same year, LLNL began CBNP using Laboratory-Directed R&D investments and a focus on biodetection. The Nunn-Lugar-Domenici Defense Against Weapons of Mass Destruction Act, passed in 1996, initiated a number of U.S. nonproliferation and counter-terrorism programs including the DOE (now NNSA) Chemical and Biological Nonproliferation Program (also known as CBNP). In 2002, the Department of Homeland Security was formed. The NNSA CBNP and many of the LLNL CBNP activities are being transferred as the new Department becomes operational. LLNL has a long history in national security including nonproliferation of weapons of mass destruction. In biology, LLNL had a key role in starting and implementing the Human Genome Project and, more recently, the Microbial Genome Program. LLNL has over 1,000 scientists and engineers with relevant expertise in biology, chemistry, decontamination, instrumentation, microtechnologies, atmospheric modeling, and field experimentation. Over 150 LLNL scientists and engineers work full time on chemical and biological national security projects.

  16. Biological Hydrogen Production Workshop

    Energy.gov [DOE]

    The U.S. Department of Energy's (DOE's) National Renewable Energy Laboratory (NREL) held a Biological Hydrogen Production Workshop on September 24–25, 2013, in Golden, Colorado. The workshop...

  17. Universal Biology, the Genetic

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Universal Biology, the Genetic Code and the First Billion Years of Life on Earth Nigel Goldenfeld University of Illinois May 25, 2016 4:00 p.m. - Wilson Hall, One West This ...

  18. Work with Biological Materials

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Work with Biological Materials Print Planning A complete Experiment Safety Sheet (ESS) is required before work can be done at the ALS. This ESS is either a part of the proposal...

  19. Genomics and Systems Biology

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Genomics and Systems Biology Los Alamos National Laboratory leads the world in computational finishing of microbial genomes Read caption + In 2013, Los Alamos scientist Richard Sayre and his team genetically modified the organisms to harvest light more efficiently for maximum production. Overview of Research and Highlights Researchers at Los Alamos National Laboratory are using their renowned expertise in genomics, computation, and experimental biology as the foundation of a dynamic systems

  20. Biological and Environmental Research

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Biological and Environmental Research Biological and Environmental Research Understanding how genomic information is translated to functional capabilities, and the roles of Earth's biogeochemical systems so we can predict climate decades or centuries into the future. Get Expertise James Bossert (505) 667-3644 Email Cathy Wilson (505) 667-0202 Email Srinivas Iyer (505) 667-2690 Email Conducting research into sustainable biofuel production, improved carbon storage, and climate driven by a

  1. Beamline 4.2.2

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    4.2.2 Print Molecular Biology Consortium Multiple-Wavelength Anomalous Diffraction (MAD) and Macromolecular Crystallography (MX) Scientific discipline: Structural biology GENERAL...

  2. BEAMLINE 4-2

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    MAIN SCIENTIFIC DISCIPLINES: Biology % TIME GENERAL USE: 100% SCHEDULING: ... station dedicated to structural biology studies primarily on non-crystalline systems. ...

  3. Beamline 5.0.2

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    or 100 K Special notes Computers for data processing and analysis, sample-changing robot available Scientific disciplines Structural biology Scientific applications Biological...

  4. Biological Systems Science Division (BSSD) | U.S. DOE Office of Science

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    (SC) Biological Systems Science Division (BSSD) Biological and Environmental Research (BER) BER Home About Research Biological Systems Science Division (BSSD) Genomic Science DOE Bioenergy Research Centers Bioimaging Technology DOE Joint Genome Institute Structural Biology Radiochemistry & Imaging Instrumentation Radiobiology: Low Dose Radiation Research DOE Human Subjects Protection Program Climate and Environmental Sciences Division (CESD) Research Abstracts Searchable Archive of BER

  5. Biological Effects of Ionizing Radiation

    DOE R&D Accomplishments

    Ingram, M.; Mason, W. B.; Whipple, G. H.; Howland, J. W.

    1952-04-07

    This report presents a review of present knowledge and concepts of the biological effects of ionizing radiations. Among the topics discussed are the physical and chemical effects of ionizing radiation on biological systems, morphological and physiological changes observed in biological systems subjected to ionizing radiations, physiological changes in the intact animal, latent changes following exposure of biological systems to ionizing radiations, factors influencing the biological response to ionizing radiation, relative effects of various ionizing radiations, and biological dosimetry.

  6. Structure of the ESCRT-II Endosomal Trafficking Complex

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    A Hurley1 1 Laboratory of Molecular Biology, National Institute of Diabetes and ... The SSRL Structural Molecular Biology Program is supported by the Department of Energy, ...

  7. Target Selection and Deselection at the Berkeley StructuralGenomics...

    Office of Scientific and Technical Information (OSTI)

    similar to those recentlysolved by the structural biology community or other centers. ... centers and the worldwidestructural biology community (94 M. pneumoniae and 86M. ...

  8. Application of Dye-Ligand Affinity Chromatography to Structural...

    Office of Scientific and Technical Information (OSTI)

    Application of Dye-Ligand Affinity Chromatography to Structural Genomics Kim, Chang Y. Los Alamos National Laboratory Applied Life Science(60) Biological Science Biological...

  9. Green Biologics | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    Biologics Jump to: navigation, search Name: Green Biologics Place: Oxfordshire, United Kingdom Sector: Biomass, Renewable Energy Product: Oxfordshire-based industrial biotech...

  10. Biological response modifiers

    SciTech Connect

    Weller, R.E.

    1991-10-01

    Much of what used to be called immunotherapy is now included in the term biological response modifiers. Biological response modifiers (BRMs) are defined as those agents or approaches that modify the relationship between the tumor and host by modifying the host's biological response to tumor cells with resultant therapeutic effects.'' Most of the early work with BRMs centered around observations of spontaneous tumor regression and the association of tumor regression with concurrent bacterial infections. The BRM can modify the host response in the following ways: Increase the host's antitumor responses through augmentation and/or restoration of effector mechanisms or mediators of the host's defense or decrease the deleterious component by the host's reaction; Increase the host's defenses by the administration of natural biologics (or the synthetic derivatives thereof) as effectors or mediators of an antitumor response; Augment the host's response to modified tumor cells or vaccines, which might stimulate a greater response by the host or increase tumor-cell sensitivity to an existing response; Decrease the transformation and/or increase differentiation (maturation) of tumor cells; or Increase the ability of the host to tolerate damage by cytotoxic modalities of cancer treatment.

  11. Advancing Environmental Flow Science: Developing Frameworks for Altered Landscapes and Integrating Efforts Across Disciplines

    DOE PAGES [OSTI]

    Brewer, Shannon; McManamay, Ryan A.; Miller, Andrew D.; Mollenhauer, Robert; Worthington, Thomas A.; Arsuffi, Tom

    2016-05-13

    Environmental flows represent a legal mechanism to balance existing and future water uses and sustain non-use values. Here, we identify current challenges, provide examples where they are important, and suggest research advances that would benefit environmental flow science. Specifically, environmental flow science would benefit by (1) developing approaches to address streamflow needs in highly modified landscapes where historic flows do not provide reasonable comparisons, (2) integrating water quality needs where interactions are apparent with quantity but not necessarily the proximate factor of the ecological degradation, especially as frequency and magnitudes of inflows to bays and estuaries, (3) providing a bettermore » understanding of the ecological needs of native species to offset the often unintended consequences of benefiting non-native species or their impact on flows, (4) improving our understanding of the non-use economic value to balance consumptive economic values, and (5) increasing our understanding of the stakeholder socioeconomic spatial distribution of attitudes and perceptions across the landscape. Environmental flow science is still an emerging interdisciplinary field and by integrating socioeconomic disciplines and developing new frameworks to accommodate our altered landscapes, we should help advance environmental flow science and likely increase successful implementation of flow standards.« less

  12. OTEC environmental biological oceanographic program

    SciTech Connect

    Hartwig, E.O.

    1981-07-01

    One of the major goals of the OTEC biological field measurement program is to assess the effect of OTEC operations on the environment. Prior understanding of the natural variability of the tropical oceanic plankton community is the most important method for determining changes due to operation of an OTEC plant. The spatial and temporal patterns of the plankton community in terms of absolute number, biomass and species composition have been investigated at potential OTEC sites. Considerable data exist which document the changes with depth of all three measurements. Diel fluctuations in number and species composition have been studied at one site. While horizontal and seasonal patterns of variability likely exist at all sites, they are subtle and remain somewhat unclear. Attempts are now being made to determine the overall trophic structure of the plankton community at these sites using these data, gut content analysis, and information already in the literature.

  13. Biological particle identification apparatus

    DOEpatents

    Salzman, Gary C.; Gregg, Charles T.; Grace, W. Kevin; Hiebert, Richard D.

    1989-01-01

    An apparatus and method for making multiparameter light scattering measurements from suspensions of biological particles is described. Fourteen of the sixteen Mueller matrix elements describing the particles under investigation can be substantially individually determined as a function of scattering angle and probing radiations wavelength, eight elements simultaneously for each of two apparatus configurations using an apparatus which incluees, in its simplest form, two polarization modulators each operating at a chosen frequency, one polarizer, a source of monochromatic electromagnetic radiation, a detector sensitive to the wavelength of radiation employed, eight phase-sensitive detectors, and appropriate electronics. A database of known biological particle suspensions can be assembled, and unknown samples can be quickly identified once measurements are performed on it according to the teachings of the subject invention, and a comparison is made with the database.

  14. BIOLOGICAL IRRADIATION FACILITY

    DOEpatents

    McCorkle, W.H.; Cern, H.S.

    1962-04-24

    A facility for irradiating biological specimens with neutrons is described. It includes a reactor wherein the core is off center in a reflector. A high-exposure room is located outside the reactor on the side nearest the core while a low-exposure room is located on the opposite side. Means for converting thermal neutrons to fast neutrons are movably disposed between the reactor core and the high and low-exposure rooms. (AEC)

  15. Mathematics and biology: The interface, challenges and opportunities

    SciTech Connect

    Levin, S.A. )

    1992-06-01

    The interface between mathematics and biology has long been a rich area of research, with mutual benefit to each supporting discipline. Traditional areas of investigation, such as population genetics, ecology, neurobiology, and 3-D reconstructions, have flourished, despite a rather meager environment for the funding of such work. In the past twenty years, the kind and scope of such interactions between mathematicians and biologists have changed dramatically, reaching out to encompass areas of both biology and mathematics that previously had not benefited. At the same time, with the closer integration of theory and experiment, and the increased reliance on high-speed computation, the costs of such research grew, though not the opportunities for funding. The perception became reinforced, both within the research community and at funding agencies, that although these interactions were expanding, they were not doing so at the rate necessary to meet the opportunities and needs. A workshop was held in Washington, DC, between April 28 and May 3, 1990 which drew together a broadly based group of researchers to synthesize conclusions from a group of working papers and extended discussions. The result is the report presented here, which we hope will provide a guide and stimulus to research in mathematical and computational biology for at least the next decade. The report identifies a number of grand challenges, representing a broad consensus among the participants.

  16. Atomic force microscopy of biological samples

    SciTech Connect

    Doktycz, Mitchel John

    2010-01-01

    The ability to evaluate structural-functional relationships in real time has allowed scanning probe microscopy (SPM) to assume a prominent role in post genomic biological research. In this mini-review, we highlight the development of imaging and ancillary techniques that have allowed SPM to permeate many key areas of contemporary research. We begin by examining the invention of the scanning tunneling microscope (STM) by Binnig and Rohrer in 1982 and discuss how it served to team biologists with physicists to integrate high-resolution microscopy into biological science. We point to the problems of imaging nonconductive biological samples with the STM and relate how this led to the evolution of the atomic force microscope (AFM) developed by Binnig, Quate, and Gerber, in 1986. Commercialization in the late 1980s established SPM as a powerful research tool in the biological research community. Contact mode AFM imaging was soon complemented by the development of non-contact imaging modes. These non-contact modes eventually became the primary focus for further new applications including the development of fast scanning methods. The extreme sensitivity of the AFM cantilever was recognized and has been developed into applications for measuring forces required for indenting biological surfaces and breaking bonds between biomolecules. Further functional augmentation to the cantilever tip allowed development of new and emerging techniques including scanning ion-conductance microscopy (SICM), scanning electrochemical microscope (SECM), Kelvin force microscopy (KFM) and scanning near field ultrasonic holography (SNFUH).

  17. Beamline 5.0.2

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    2 Print Berkeley Center for Structural Biology (BCSB) Multiple-wavelength anomalous diffraction (MAD) and monochromatic protein crystallography Scientific discipline: Structural...

  18. Beamline 8.2.1

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    1 Print Berkeley Center for Structural Biology (BCSB) Multiple-Wavelength Anomalous Diffraction (MAD) and Macromolecular Crystallography (MX) Scientific discipline: Structural...

  19. Abengoa Mojave Final Biological Opinion

    Energy.gov [DOE]

    Biological Opinion on Mojave Solar, LLC's Mojave Solar Project, San Bernardino County, California (8-8-11-F-3)

  20. Elements in biological AMS

    SciTech Connect

    Vogel, J.S.; McAninch, J.; Freeman, S.

    1996-08-01

    AMS (Accelerator Mass Spectrometry) provides high detection sensitivity for isotopes whose half-lives are between 10 years and 100 million years. {sup 14}C is the most developed of such isotopes and is used in tracing natural and anthropogenic organic compounds in the Earth`s biosphere. Thirty-three elements in the main periodic table and 17 lanthanides or actinides have long lived isotopes, providing potential tracers for research in elemental biochemistry. Overlap of biologically interesting heavy elements and possible AMS tracers is discussed.

  1. Biological hydrogen photoproduction

    SciTech Connect

    Nemoto, Y.

    1995-09-01

    Following are the major accomplishments of the 6th year`s study of biological hydrogen photoproduction which were supported by DOE/NREL. (1) We have been characterizing a biological hydrogen production system using synchronously growing aerobically nitrogen-fixing unicellular cyanobacterium, Synechococcus sp. Miami BG 043511. So far it was necessary to irradiate the cells to produce hydrogen. Under darkness they did not produce hydrogen. However, we found that, if the cells are incubated with oxygen, they produce hydrogen under the dark. Under 80% argon + 20% oxygen condition, the hydrogen production activity under the dark was about one third of that under the light + argon condition. (2) Also it was necessary so far to incubate the cells under argon atmosphere to produce hydrogen in this system. Argon treatment is very expensive and should be avoided in an actual hydrogen production system. We found that, if the cells are incubated at a high cell density and in a container with minimum headspace, it is not necessary to use argon for the hydrogen production. (3) Calcium ion was found to play an important role in the mechanisms of protection of nitrogenase from external oxygen. This will be a clue to understand the reason why the hydrogen production is so resistant to oxygen in this strain. (4) In this strain, sulfide can be used as electron donor for the hydrogen production. This result shows that waste water can be used for the hydrogen production system using this strain.

  2. Biological Performance Assessment

    Energy Science and Technology Software Center

    2013-07-09

    The BioPA provides turbine designers with a set of tools that can be used to assess biological risks of turbines during the design phase, before expensive construction begins. The toolset can also be used to assess existing installations under a variety of operating conditions, supplementing data obtained through expensive field testing. The BioPA uses computational fluid dynamics (CFD) simulations of a turbine design to quantify the exposure of passing fish to a set of knownmore » injury mechanisms. By appropriate sampling of the fluid domain, the BioPA assigns exposure probabilities to each mechanism. The exposure probabilities are combined with dose-response data from laboratory stress studies of fish to produce a set of biological BioPA Scores. These metrics provide an objective measure that can be used to compare competing turbines or to refine a new design. The BioPA process can be performed during the turbine design phase and is considerably less expensive than prototype-scale field testing.« less

  3. Structure of a mutant [beta] toxin from Staphylococcus aureus...

    Office of Scientific and Technical Information (OSTI)

    Language: ENGLISH Subject: 59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUSMATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; CRYSTAL LATTICES; CRYSTAL STRUCTURE; ...

  4. 2013 Biological Hydrogen Production Workshop Summary Report ...

    Energy Saver

    Biological Hydrogen Production Workshop Summary Report 2013 Biological Hydrogen Production Workshop Summary Report November 2013 summary report for the 2013 Biological Hydrogen ...

  5. Biological Lignin Depolymerization Presentation for BETO 2015...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    depolymerization with new synthetic biology techniques and new process concepts in biological funneling High-Level Objective: * Employ biology for depolymerization and aromatic ...

  6. Update on the Pfam5000 Strategy for Selection of StructuralGenomics...

    Office of Scientific and Technical Information (OSTI)

    Country of Publication: United States Language: English Subject: 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; BIOLOGY; MEDICINE; PRODUCTION; PROTEIN STRUCTURE; PROTEINS; ...

  7. Continuous mutual improvement of macromolecular structure models...

    Office of Scientific and Technical Information (OSTI)

    ... Country of Publication: United States Language: English Subject: 59 BASIC BIOLOGICAL SCIENCES; 96 KNOWLEDGE MANAGEMENT AND PRESERVATION structure determination; model quality; data ...

  8. Crystal Structures of Phosphite Dehydrogenase Provide Insights...

    Office of Scientific and Technical Information (OSTI)

    Country of Publication: United States Language: ENGLISH Subject: 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; CATALYSTS; CRYSTAL STRUCTURE; ENZYMES; NICOTINAMIDE; ...

  9. Biochemical Process Improvements & Synthetic Biology Validation...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Process Improvements & Synthetic Biology Validation Projects This presentation ... Develop efficient, inexpensive synthetic biology methods and tools to convert biomass into ...

  10. Biological Safety | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Biological Select Agents Inspection Report: IG-0681, Concerns Regarding a Non-Viable (Dead) "Anthrax Sport" Research Project at the Oak Ridge National Laboratory Inspection ...

  11. Biological & Environmental Research Abstracts Database

    Office of Scientific and Technical Information (OSTI)

    Welcome to the Biological and Environmental Research Abstracts Database The U.S. ... This database contains abstracts of research projects supported by the program. Work was ...

  12. Biology and Medicine Division annual report, 1987

    SciTech Connect

    Not Available

    1988-04-01

    Modern biology is characterized by rapid change. The development of new tools and the results derived from their application to various biological systems require significant shifts in our concepts and the strategies that are adopted to analyze and elucidate mechanisms. In parallel with exciting new scientific developments our organizational structure and programmatic emphases have altered. These changes and developments have enabled the life sciences at LBL to be better positioned to create and respond to new opportunities. The work summarized in this annual report reflects a vital multifaceted research program that is in the vanguard of the areas represented. We are committed to justifying the confidence expressed by LBL through the new mission statement and reorganizational changes designed to give greater prominence to the life sciences.

  13. Biological applications of ultraviolet free-electron lasers

    SciTech Connect

    Sutherland, J.C.

    1997-10-01

    This review examines the possibilities for biological research using the three ultraviolet free-electron lasers that are nearing operational status in the US. The projected operating characteristics of major interest in biological research of the free-electron lasers at Brookhaven National Laboratory, the Thomas Jefferson National Accelerator Facility, and Duke University are presented. Experimental applications in the areas of far- and vacuum ultraviolet photophysics and photochemistry, structural biology, environmental photobiology, and medical research are discussed and the prospects for advances in these areas, based upon the characteristics of the new ultraviolet free-electron lasers, are evaluated.

  14. Biological warfare in the littorals. Final report

    SciTech Connect

    Larsen, R.W.

    1997-05-01

    Biological warfare (BW) has emerged as a significant threat to military operations and is particularly challenging at the operational level of warfare in a littoral environment. There are compelling reasons why an operational commander should be concerned about BW: global proliferation of biotechnology and biological weapons capabilities; suitability of BW for disrupting force projection across the littorals; and the vulnerability of American, allied and coalition forces to BW. The threat of facing an adversary capable and willing to use biological weapons will influence the commander`s application of the operational art across the six operational functions. Degradation of operational tempo, effects of psychological responses among the force, and stress on the organizational structure may challenge the command and control process. Operational intelligence will demand robust integration of technical analysis, intentions and warnings, meteorological information, and medical intelligence. The maneuver and movement processes will be taxed to function effectively when ports and airfields offer such lucrative BW targets. Biological weapons may dictate the location of operational fires assets as well as the make-up of the target lists. Operational logistics assumes great importance in the medical functions, decontamination processes, and troop replacement and unit reconstitution. Operational protection encompasses nearly every aspect of BW defense and will demand a balance between what is necessary and what is possible to protect. As daunting as the challenges appear, the operational-level commander has at his disposal many tools necessary to prepare for biological warfare in the littorals. Ultimately, the commander must convince his force, his allies, and his enemies that the command can fight effectively in a BW environment, on land and sea.

  15. Plant Molecular Biology 2008 Gordon Research Conference - July 13-18, 2008

    SciTech Connect

    Richard M. Amasino

    2009-08-28

    The Plant Molecular Biology Conference has traditionally covered a breadth of exciting topics and the 2008 conference will continue in that tradition. There will be sessions on metabolism; new methods to study genomes, proteomes and metabolomes; plant-microbe interactions; plant hormones; epigenetics. A new topic for the conference this year will be bioenergy. Thus this conference will bring together a range of disciplines to foster the exchange ideas and to permit the participants to learn of the latest developments and ideas in diverse areas of plant biology. The conference provides an excellent opportunity for individuals to discuss their research because additional speakers in each session will be selected from submitted abstracts. There will also be a poster session each day for a two-hour period prior to dinner.

  16. 2010 Tetrapyrroles, Chemistry & Biology of Gordon Research Conference

    SciTech Connect

    Angela Wilks

    2010-07-30

    The objective of the Chemistry & Biology of Tetrapyrroles Gordon Conference is to bring together researchers from diverse disciplines that otherwise would not interact. By bringing biologists, chemists, engineers and clinicians with a common interest in tetrapyrroles the conference provides a forum for cross-disciplinary ideas and collaboration. The perspective provided by biologists, chemists, and clinicians working in fields such as newly discovered defects in human porphyrin metabolism, the myriad of strategies for light harvesting in photosynthetic organisms, novel tetrapyrroles that serve as auxiliary chromophores or enzyme cofactors, synthetic strategies in the design of novel tetrapyrrole scaffolds, and tetrapyrrole based cell signaling and regulatory systems, makes this conference unique in the field. Over the years the growing evidence for the role of tetrapyrroles and their reactive intermediates in cell signaling and regulation has been of increasing importance at this conference. The 2010 conference on Chemistry & Biology of Tetrapyrroles will focus on many of these new frontiers as outlined in the preliminary program listed. Speakers will emphasize unpublished results and new findings in the field. The oral sessions will be followed by the highly interactive afternoon poster sessions. The poster sessions provide all conferees with the opportunity to present their latest research and to exchange ideas in a more informal setting. As in the past, this opportunity will continue during the nightly social gathering that takes place in the poster hall following the evening lectures. All conferees are encouraged to submit and present posters. At the conference the best poster in the areas of biology, chemistry and medicine will be selected by a panel of previous conference chairs.

  17. Beamline 8.3.1

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Ambient or 100 K Special notes Computers for data processing and analysis, robotic sample handling available Scientific disciplines Structural biology Scientific...

  18. Beamline 8.3.1

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    3.1 Print Multiple-wavelength anomalous diffraction (MAD) and macromolecular crystallography (MX) Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational...

  19. Experimental Station 14-2 | Stanford Synchrotron Radiation Lightsource

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    2 NA Status In Design Supported Techniques White Light Station Main Scientific Disciplines Structural Molecular Biology Beam Line Specifications Source Bending Magnet Energy Range ...

  20. Beamline 5.0.1

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    or 100 K Special notes Computers for data processing and analysis, sample-changing robot available Scientific disciplines Structural biology Scientific applications...

  1. Beamline 5.0.3

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    or 100 K Special notes Computers for data processing and analysis, sample-changing robot available Scientific disciplines Structural biology Scientific applications...

  2. Beamline 8.3.1

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    environment Ambient or 100 K Special notes Computers for data processing and analysis, robotic sample handling available Scientific disciplines Structural biology Scientific...

  3. Beamline 12.3.1

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    for small samples with flux reduced to 1% and 12%, respectively. 120 m x 120 m Gaussian uncollimated beam shape at sample. Scientific discipline Structural biology...

  4. Experimental Station 14-1 | Stanford Synchrotron Radiation Lightsource

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Single wavelength anomalous diffraction (SAD) Main Scientific Disciplines Structural Molecular Biology Beam Line Specifications Source Bending Magnet Source Bending magnet, side...

  5. Biological Science | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Biological Science Biological Science The protozoan Plasmodium falciparum gliding through a cell in the gut of a mosquito, its primary host. Although five different species of Plasmodium can cause malaria, Plasmodium falciparum causes the most severe disease. | Photo courtesy of Wikipedia Commons. <a href="http://energy.gov/articles/malaria-researchers-find-weakness-global-killer">Read more</a> The protozoan Plasmodium falciparum gliding through a cell in the gut of a

  6. SINGLE MOLECULE APPROACHES TO BIOLOGY, 2010 GORDON RESEARCH CONFERENCE, JUNE 27-JULY 2, 2010, ITALY

    SciTech Connect

    Professor William Moerner

    2010-07-09

    Tuscany, provides an avenue for scientists from different disciplines to interact and brainstorm and promotes cross-disciplinary collaborations directed toward compelling biological problems.

  7. Systems Biology in Prokaryote - Eukaryote Symbiosis | Stanford Synchrotron

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Radiation Lightsource Systems Biology in Prokaryote - Eukaryote Symbiosis Monday, June 25, 2012 - 12:00pm SLAC, SSRL Main Conference Room, 137-322 Allen M. Orville, Brookhaven National Laboratory Frontier challenges for macromolecular crystallography (MX) now include determining structures of trapped reactive intermediates, large macromolecules and viruses, membrane proteins, protein-protein complexes, and protein-nucleic acid complexes. Although structure and function are intimately linked,

  8. Artificial photosynthesis combines biology with technology for...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    biology with technology for sustainable energy transformation Authors: Moore, T.A., Moore, A.L., and Gust, D. Title: Artificial photosynthesis combines biology with ...

  9. Autofermentative Biological Hydrogen Production by Cyanobacteria...

    Energy.gov [DOE] (indexed site)

    More Documents & Publications 2013 Biological Hydrogen Production Workshop Summary Report Renewable Hydrogen Production from Biological Systems Anthropogenic CO2 as a Feedstock for ...

  10. Repurposing the translation apparatus for synthetic biology ...

    Office of Scientific and Technical Information (OSTI)

    biology Citation Details In-Document Search This content will become publicly available on July 14, 2017 Title: Repurposing the translation apparatus for synthetic biology ...

  11. Innovative Breakthrough Demonstrated for Biological Ethanol Production...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Innovative Breakthrough Demonstrated for Biological Ethanol Production Innovative Breakthrough Demonstrated for Biological Ethanol Production June 30, 2015 - 11:43am Addthis ...

  12. Thin film composition with biological substance and method of making

    SciTech Connect

    Campbell, A.A.; Song, L.

    1999-09-28

    The invention provides a thin-film composition comprising an underlying substrate of a first material including a plurality of attachment sites; a plurality of functional groups chemically attached to the attachment sites of the underlying substrate; and a thin film of a second material deposited onto the attachment sites of the underlying substrate, and a biologically active substance deposited with the thin-film. Preferably the functional groups are attached to a self assembling monolayer attached to the underlying substrate. Preferred functional groups attached to the underlying substrate are chosen from the group consisting of carboxylates, sulfonates, phosphates, optionally substituted, linear or cyclo, alkyl, alkene, alkyne, aryl, alkylaryl, amine, hydroxyl, thiol, silyl, phosphoryl, cyano, metallocenyl, carbonyl, and polyphosphate. Preferred materials for the underlying substrate are selected from the group consisting of a metal, a metal alloy, a plastic, a polymer, a proteic film, a membrane, a glass or a ceramic. The second material is selected from the group consisting of inorganic crystalline structures, inorganic amorphous structures, organic crystalline structures, and organic amorphous structures. Preferred second materials are phosphates, especially calcium phosphates and most particularly calcium apatite. The biologically active molecule is a protein, peptide, DNA segment, RNA segment, nucleotide, polynucleotide, nucleoside, antibiotic, antimicrobial, radioisotope, chelated radioisotope, chelated metal, metal salt, anti-inflammatory, steroid, nonsteroid anti-inflammatory, analgesic, antihistamine, receptor binding agent, or chemotherapeutic agent, or other biologically active material. Preferably the biologically active molecule is an osteogenic factor consisting of the compositions listed above.

  13. Thin film composition with biological substance and method of making

    DOEpatents

    Campbell, Allison A.; Song, Lin

    1999-01-01

    The invention provides a thin-film composition comprising an underlying substrate of a first material including a plurality of attachment sites; a plurality of functional groups chemically attached to the attachment sites of the underlying substrate; and a thin film of a second material deposited onto the attachment sites of the underlying substrate, and a biologically active substance deposited with the thin-film. Preferably the functional groups are attached to a self assembling monolayer attached to the underlying substrate. Preferred functional groups attached to the underlying substrate are chosen from the group consisting of carboxylates, sulfonates, phosphates, optionally substituted, linear or cyclo, alkyl, alkene, alkyne, aryl, alkylaryl, amine, hydroxyl, thiol, silyl, phosphoryl, cyano, metallocenyl, carbonyl, and polyphosphate. Preferred materials for the underlying substrate are selected from the group consisting of a metal, a metal alloy, a plastic, a polymer, a proteic film, a membrane, a glass or a ceramic. The second material is selected from the group consisting of inorganic crystalline structures, inorganic amorphus structures, organic crystalline structures, and organic amorphus structures. Preferred second materials are phosphates, especially calcium phosphates and most particularly calcium apatite. The biologically active molecule is a protein, peptide, DNA segment, RNA segment, nucleotide, polynucleotide, nucleoside, antibiotic, antimicrobal, radioisotope, chelated radioisotope, chelated metal, metal salt, anti-inflamatory, steriod, nonsteriod anti-inflammatory, analgesic, antihistamine, receptor binding agent, or chemotherapeutic agent, or other biologically active material. Preferably the biologically active molecule is an osteogenic factor the compositions listed above.

  14. DOE Chair of Excellence in Environmental Disciplines-Final Technical Report

    SciTech Connect

    Kurunganty, Sastry; Loran, Roberto; Roque-Malherbe, Rolando; Hijazi, Yazan; Nieto, Santander; Gomez, Will A.; Duconge, Jose; Cotto, María del C.; Muniz, Carlos; Diaz, Francisco J.; Neira, Carlos F.; Marquez, Francisco; Del Valle, W.; Thommes, M.

    2014-02-19

    Characterization of low Refractive Index Aerogel Silica for Cherenkov Counters- Dr. Rolando Roque-Malherbe Sub-project PI, Dr. Jose Duconge Sub-project Co-PI, Dr. Santander Nieto Assistant Researcher, Francisco Diaz and Carlos Neira Associate Researchers. The initial aim of this sub-project was changed to the synthesis and characterization of extremely high specific surface area aerogel silica for gas storage. A high specific surface area silica gel that has applications in gas drying, cleaning operation useful in nuclear industry in process was developed. Sub-Project 2: Investigation Study of Magnetic and Electronic Transport Properties at Material Interfaces in Magnetic Multilayer Heterostructure using Gd. – Dr. Yazan Hijazi, Sub-project Co-PI. UT developed the capability and infrastructure to produce high quality thin-film magnetic films and magnetic multilayer structures with fine control over film quality and thickness using sputter deposition capability to perform in-house electric and magnetic characterization of these films. The research experimentally quantified the effect of Gd incorporation within the magnetic multilayer structure and produce magnetic media with exchanged decoupled multilevel magnetic anisotropy. From September 2006 to September 2011 the Massie Chair produced nineteen (19) publications, (including 3 books), five (5) presentations and three (3) international conferences abstracts. A total of fourteen (14) undergraduates and (6) graduate students acquired research experience. Two Ph.D. students presented their dissertations on topics related to nuclear energy and graduated as follows: María Cotto (May 2009) and Eric Calderón (May 2011). Five of the participating undergraduate students graduated: Ramon Polanco (BSME, May 2009), Jason Pérez (BSEE, May 2008), Rafael Colón (BSME, May 2008), Jessenia Marfisi (BS Chemistry, May 2008). Eleven (11) students were sent to National Laboratories (LANL, SNL and LLNL), NNSA and DoE facilities for summer

  15. Data publication with the structural biology data grid supports...

    Office of Scientific and Technical Information (OSTI)

    J. ; Bonvin, Alexandre M.J. J. ; Borek, Dominika ; Brett, Tom J. ; Caflisch, Amedeo ; Chang, Chung-I more ; Chazin, Walter J. ; Corbett, Kevin D. ; Cosgrove, Michael S. ; ...

  16. The Biological Implications of the PP2A Crystal Structure

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    groups are not removed in needed situations) is associated with multiple human cancers, Alzheimer's Disease, and increased susceptibility to pathogen infections. To shed light on...

  17. Frontiers of Plant Cell Biology: Signals and Pathways, System-Based Approaches 22nd Symposium in Plant Biology (University of California-Riverside)

    SciTech Connect

    Minorsky, Peter V.

    2003-06-01

    The symposium ''Frontiers of Plant Cell Biology: Signals and Pathways, Systems-Based Approaches'' was held January 15-18, 2003 at the Riverside Convention Center in Riverside, California. The host organization for the symposium was the Center for Plant Cell Biology (CEPCEB) at the University of California, Riverside (UCR). The meeting, focusing on systems-based approaches to plant cell biology research, was the first of this kind in the field of plant biology. The speakers and nearly 100 posters placed emphasis on recent developments in plant cellular biology and molecular genetics, particularly those employing emerging genomic tools, thereby sharing the most current knowledge in the field and stimulating future advances. In attendance were many well-established scientists and young investigators who approach plant cell biology from different but complementary conceptual and technical perspectives. Indeed, many disciplines are converging in the field of cell biology, producing synergies that will enable plant scientists to determine the function of gene products in the context of living cells in whole organisms. New, cross-disciplinary collaborations, as well as the involvement of computer scientists and chemists in plant biology research, are likely additional outcomes of the symposium. The program included 39 invited session speakers and workshop/panel speakers. Sessions were convened on the following themes: Cell-Cell Communication; Protein Trafficking; Cell Surface, Extracellular Matrix and Cell Wall; Signal Transduction; Signal Transduction and Proteosome; and Systems-Based Approaches to Plant Cell Biology. Workshops on Chemical Genetics and Visual Microscopy were also presented. Abstracts from each of the speaker presentations, as well as the posters presented at the meeting were published in a program booklet given to the 239 faculty members, researchers, postdoctoral scientists and graduate students in attendance. The booklet thus serves as a reference for

  18. Biology Division progress report, October 1, 1993--September 30, 1995

    SciTech Connect

    1995-10-01

    This Progress Report summarizes the research endeavors of the Biology Division of the Oak Ridge National Laboratory during the period October 1, 1993, through September 30, 1995. The report is structured to provide descriptions of current activities and accomplishments in each of the Division`s major organizational units. Lists of information to convey the entire scope of the Division`s activities are compiled at the end of the report. Attention is focused on the following research activities: molecular, cellular, and cancer biology; mammalian genetics and development; genome mapping program; and educational activities.

  19. Storage by Scientific Discipline

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Heat & Cool » Water Heating » Storage Water Heaters Storage Water Heaters Consider energy efficiency when selecting a conventional storage water heater to avoid paying more over its lifetime. | Photo courtesy of ©iStockphoto/JulNichols. Consider energy efficiency when selecting a conventional storage water heater to avoid paying more over its lifetime. | Photo courtesy of ©iStockphoto/JulNichols. Conventional storage water heaters remain the most popular type of water heating system

  20. Advances in imaging secondary ion mass spectrometry for biological samples

    DOE PAGES [OSTI]

    Boxer, Steven G.; Kraft, Mary L.; Weber, Peter K.

    2008-12-16

    Imaging mass spectrometry combines the power of mass spectrometry to identify complex molecules based on mass with sample imaging. Recent advances in secondary ion mass spectrometry have improved sensitivity and spatial resolution, so that these methods have the potential to bridge between high-resolution structures obtained by X-ray crystallography and cyro-electron microscopy and ultrastructure visualized by conventional light microscopy. Following background information on the method and instrumentation, we address the key issue of sample preparation. Because mass spectrometry is performed in high vacuum, it is essential to preserve the lateral organization of the sample while removing bulk water, and this hasmore » been a major barrier for applications to biological systems. Furthermore, recent applications of imaging mass spectrometry to cell biology, microbial communities, and biosynthetic pathways are summarized briefly, and studies of biological membrane organization are described in greater depth.« less

  1. Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase...

    Office of Scientific and Technical Information (OSTI)

    Resource Type: Journal Article Resource Relation: Journal Name: Acta Crystallographica. Section F, Structural Biology Communications; Journal Volume: 72; Journal Issue: Pt 6; Other ...

  2. Purification, crystallization and structural elucidation ofD...

    Office of Scientific and Technical Information (OSTI)

    Resource Type: Journal Article Resource Relation: Journal Name: Acta Crystallographica. Section F, Structural Biology Communications; Journal Volume: 72; Journal Issue: (1) ; 01, ...

  3. Structural bases of dimerization of yeast telomere protein Cdc13...

    Office of Scientific and Technical Information (OSTI)

    Country of Publication: United States Language: ENGLISH Subject: 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; CRYSTAL STRUCTURE; DIMERIZATION; DNA POLYMERASES; ...

  4. Ultrafast myoglobin structural dynamics observed with an X-ray...

    Office of Scientific and Technical Information (OSTI)

    Univ. of Palermo (Italy). Dept. of Physics. Univ. of Grenoble Alpes and Institute of Structural Biology (France) SLAC National Accelerator Laboratory, Menlo Park, CA (Untied ...

  5. Algal Biology Toolbox Workshop Summary Report

    Office of Energy Efficiency and Renewable Energy (EERE)

    The Algal Biology Toolbox Workshop Summary Report summarizes an event hosted by the U.S. Department of Energy’s Bioenergy Technologies Office’s Advanced Algal Systems Program in May 2016. The purpose of the Algal Biology Toolbox Workshop was to collect input from experts in the field of algal biology regarding (1) the current state of algal biological tools, including our understanding of algal biology and biochemistry, available molecular toolboxes, omics databases, and other resources; (2) challenges to developing and applying a full suite of biological tools to improve algae performance and system robustness; and (3) strategies to advance progress toward commercial algal biofuels.

  6. 2013 METALS IN BIOLOGY GORDON RESEARCH CONFERENCE, JANUARY 20-25, 2013

    SciTech Connect

    Rosenzweig, Amy

    2013-01-25

    Typical topics for lectures and posters include: biochemical and biophysical characterization of new metal containing proteins, enzymes, nucleic acids, factors, and chelators from all forms of life; synthesis, detailed characterization, and reaction chemistry of biomimetic compounds; novel crystal and solution structures of biological molecules and synthetic metal-chelates; discussions of the roles that metals play in medicine, maintenance of the environment, and biogeochemical processes; metal homeostasis; application of theory and computations to the structure and mechanism of metal-containing biological systems; and novel applications of spectroscopy to metals in biological systems.

  7. Algal Biology Toolbox Workshop Summary Report

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Biology Toolbox Workshop Summary Report San Diego, California May 2016 Summary report from the May 24-25, 2016, Algal Biology Toolbox Workshop in San Diego, California Workshop and ...

  8. Biology Division progress report, October 1, 1991--September 30, 1993

    SciTech Connect

    Hartman, F.C.; Cook, J.S.

    1993-10-01

    This Progress Report summarizes the research endeavors of the Biology Division of the Oak Ridge National Laboratory during the period October 1, 1991, through September 30, 1993. The report is structured to provide descriptions of current activities and accomplishments in each of the Division`s major organizational units. Lists of information to convey the entire scope of the Division`s activities are compiled at the end of the report.

  9. Rapid classification of biological components

    DOEpatents

    Thompson, Vicki S.; Barrett, Karen B.; Key, Diane E.

    2013-10-15

    A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do not form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to a subject's identity.

  10. Rapid classification of biological components

    DOEpatents

    Thompson, Vicki S.; Barrett, Karen B.; Key, Diane E.

    2010-03-23

    A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do not form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to a subject's identity.

  11. Rapid classification of biological components

    DOEpatents

    Thompson, Vicki S.; Barrett, Karen B.; Key, Diane E.

    2006-01-24

    A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, cocaine, methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens to the surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.

  12. Rapid classification of biological components

    SciTech Connect

    Thompson, Vicki S.; Barrett, Karen B.; Key, Diane E.

    2010-03-23

    A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens of the surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do not form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to a subject's identity.

  13. Photo of the Week: Students from Roosevelt Middle School win...

    Office of Environmental Management (EM)

    disciplines, including biology, chemistry, earth science, physics, astronomy and math. ... disciplines, including biology, chemistry, earth science, physics, astronomy and math. ...

  14. Impact of Radiation Biology on Fundamental Insights in Biology

    DOE R&D Accomplishments

    Setlow, Richard B.

    1982-07-27

    Research supported by OHER [Office of Health and Environmental Research] and its predecessors has as one of its major goals an understanding of the effects of radiation at low doses and dose rates on biological systems, so as to predict their effects on humans. It is not possible to measure such effects directly. They must be predicted from basic knowledge on how radiation affects cellular components such as DNA and membranes and how cells react to such changes. What is the probability of radiation producing human mutations and what are the probabilities of radiation producing cancer? The end results of such studies are radiation exposure standards for workers and for the general population. An extension of these goals is setting standards for exposure to chemicals involved in various energy technologies. This latter problem is much more difficult because chemical dosimetry is a primitive state compared to radiation dosimetry.

  15. Impact of Radiation Biology on Fundamental in Biology

    SciTech Connect

    Setlow, Richard B.

    1983-01-20

    Research supported by OHER and its predecessors has as one of its major goals an understanding of the effects of radiation at low doses and dose rates on biological systems, so as to predict their effects on humans. It is not possible to measure such effects directly. They must be predicted from basic knowledge on how radiation affects cellular components such as DNA and membranes and how cells react to such changes. What is the probability of radiation producing human mutations and what are the probabilities of radiation producing cancer? The end results of such studies are radiation exposure standards for workers and for the general population. An extension of these goals is setting standards for exposure to chemicals involved in various energy technologies. This latter problem is much more difficult because chemical dosimetry is is a primitive state compared to radiation dosimetry.

  16. Method of measurement in biological systems

    DOEpatents

    Turteltaub, K.W.; Vogel, J.S.; Felton, J.S.; Gledhill, B.L.: Davis, J.C.; Stanker, L.H.

    1993-05-11

    A method is disclosed of quantifying molecules in biological substances, comprising: selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere; preparing a long-lived radioisotope labeled reactive chemical specie; administering the chemical specie to the biological host in doses sufficiently low to avoid significant overt damage to the biological system; allowing a period of time to elapse sufficient for dissemination and interaction of the chemical specie with the host throughout the biological system of the host; isolating a reacted fraction of the biological substance from the host in a manner sufficient to avoid contamination of the substance from extraneous sources; converting the fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation; and measuring the radioisotope concentration in the material by means of direct isotopic counting.

  17. Invited Review Article: Advanced light microscopy for biological space research

    SciTech Connect

    De Vos, Winnok H.; Beghuin, Didier; Schwarz, Christian J.; Jones, David B.; Loon, Jack J. W. A. van

    2014-10-15

    As commercial space flights have become feasible and long-term extraterrestrial missions are planned, it is imperative that the impact of space travel and the space environment on human physiology be thoroughly characterized. Scrutinizing the effects of potentially detrimental factors such as ionizing radiation and microgravity at the cellular and tissue level demands adequate visualization technology. Advanced light microscopy (ALM) is the leading tool for non-destructive structural and functional investigation of static as well as dynamic biological systems. In recent years, technological developments and advances in photochemistry and genetic engineering have boosted all aspects of resolution, readout and throughput, rendering ALM ideally suited for biological space research. While various microscopy-based studies have addressed cellular response to space-related environmental stressors, biological endpoints have typically been determined only after the mission, leaving an experimental gap that is prone to bias results. An on-board, real-time microscopical monitoring device can bridge this gap. Breadboards and even fully operational microscope setups have been conceived, but they need to be rendered more compact and versatile. Most importantly, they must allow addressing the impact of gravity, or the lack thereof, on physiologically relevant biological systems in space and in ground-based simulations. In order to delineate the essential functionalities for such a system, we have reviewed the pending questions in space science, the relevant biological model systems, and the state-of-the art in ALM. Based on a rigorous trade-off, in which we recognize the relevance of multi-cellular systems and the cellular microenvironment, we propose a compact, but flexible concept for space-related cell biological research that is based on light sheet microscopy.

  18. Biological and Environmental Research (BER) Homepage | U.S. DOE...

    Office of Science (SC)

    EXPLORING the frontiers of genome-enabled biology. Understanding Complex Biological and ... and inquiry in atmospheric chemistry and physics, ecology, biology, and biogeochemistry. ...

  19. Breaking the Biological Barriers to Cellulosic Ethanol, June...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Breaking the Biological Barriers to Cellulosic ...

  20. Electrostatic thin film chemical and biological sensor

    DOEpatents

    Prelas, Mark A.; Ghosh, Tushar K.; Tompson, Jr., Robert V.; Viswanath, Dabir; Loyalka, Sudarshan K.

    2010-01-19

    A chemical and biological agent sensor includes an electrostatic thin film supported by a substrate. The film includes an electrostatic charged surface to attract predetermined biological and chemical agents of interest. A charge collector associated with said electrostatic thin film collects charge associated with surface defects in the electrostatic film induced by the predetermined biological and chemical agents of interest. A preferred sensing system includes a charge based deep level transient spectroscopy system to read out charges from the film and match responses to data sets regarding the agents of interest. A method for sensing biological and chemical agents includes providing a thin sensing film having a predetermined electrostatic charge. The film is exposed to an environment suspected of containing the biological and chemical agents. Quantum surface effects on the film are measured. Biological and/or chemical agents can be detected, identified and quantified based on the measured quantum surface effects.

  1. Developing Biological Specifications for Fish Friendly Turbines |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy Developing Biological Specifications for Fish Friendly Turbines Developing Biological Specifications for Fish Friendly Turbines This factsheet explains studies conducted in a highly reproducible manner to examine the biological effects to fish exposed to a shear environment in the laboratory. bio_specific_fish_friendly_turbines.pdf (59.94 KB) More Documents & Publications Environmental Effects of Hydrokinetic Turbines on Fish: Desktop and Laboratory Flume Studies

  2. Method for photo-altering a biological system to improve biological effect

    DOEpatents

    Hill, Richard A.; Doiron, Daniel R.; Crean, David H.

    2000-08-01

    Photodynamic therapy is a new adjunctive therapy for filtration surgery that does not use chemotherapy agents or radiation, but uses pharmacologically-active sensitizing compounds to produce a titratable, localized, transient, post operative avascular conjunctiva. A photosensitizing agent in a biological system is selectively activated by delivering the photosensitive agent to the biological system and laser activating only a spatially selected portion of the delivered photosensitive agent. The activated portion of the photosensitive agent reacts with the biological system to obtain a predetermined biological effect. As a result, an improved spatial disposition and effectuation of the biological effect by the photosensitive agent in the biological system is achieved.

  3. A brief history of the Biology Complex

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Complex Most of the "Biology" buildings were constructed in early 1945 and were built as expansion capability for the uranium preparation operations being conducted across the...

  4. Autofermentative Biological Hydrogen Production by Cyanobacteria

    Office of Energy Efficiency and Renewable Energy (EERE)

    Presentation by Charles Dismukes, Rutgers University, at the Biological Hydrogen Production Workshop held September 24-25, 2013, at the National Renewable Energy Laboratory in Golden, Colorado.

  5. Genomes, Phylogeny, and Evolutionary Systems Biology

    SciTech Connect

    Medina, Monica

    2005-03-25

    With the completion of the human genome and the growing number of diverse genomes being sequenced, a new age of evolutionary research is currently taking shape. The myriad of technological breakthroughs in biology that are leading to the unification of broad scientific fields such as molecular biology, biochemistry, physics, mathematics and computer science are now known as systems biology. Here I present an overview, with an emphasis on eukaryotes, of how the postgenomics era is adopting comparative approaches that go beyond comparisons among model organisms to shape the nascent field of evolutionary systems biology.

  6. Molecular biology of signal transduction in plants

    SciTech Connect

    Not Available

    1991-01-01

    This volume contains abstracts of oral presentations and poster sessions of the 1991 Cold Springs Harbor Meeting entitled Molecular Biology of Signal Transduction in Plants.

  7. Developing Biological Specifications for Fish Friendly Turbines

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Biological Specifications for Fish Friendly Turbines The U.S. Department of Energy's ... which environmen- tal attributes, such as entrainment survival for fish, are emphasized. ...

  8. Novel nuclear magnetic resonance techniques for studying biological molecules

    SciTech Connect

    Laws, David D.

    2000-06-01

    Over the fifty-five year history of Nuclear Magnetic Resonance (NMR), considerable progress has been made in the development of techniques for studying the structure, function, and dynamics of biological molecules. The majority of this research has involved the development of multi-dimensional NMR experiments for studying molecules in solution, although in recent years a number of groups have begun to explore NMR methods for studying biological systems in the solid-state. Despite this new effort, a need still exists for the development of techniques that improve sensitivity, maximize information, and take advantage of all the NMR interactions available in biological molecules. In this dissertation, a variety of novel NMR techniques for studying biomolecules are discussed. A method for determining backbone ({phi}/{psi}) dihedral angles by comparing experimentally determined {sup 13}C{sub a}, chemical-shift anisotropies with theoretical calculations is presented, along with a brief description of the theory behind chemical-shift computation in proteins and peptides. The utility of the Spin-Polarization Induced Nuclear Overhauser Effect (SPINOE) to selectively enhance NMR signals in solution is examined in a variety of systems, as are methods for extracting structural information from cross-relaxation rates that can be measured in SPINOE experiments. Techniques for the production of supercritical and liquid laser-polarized xenon are discussed, as well as the prospects for using optically pumped xenon as a polarizing solvent. In addition, a detailed study of the structure of PrP 89-143 is presented. PrP 89-143 is a 54 residue fragment of the prion proteins which, upon mutation and aggregation, can induce prion diseases in transgenic mice. Whereas the structure of the wild-type PrP 89-143 is a generally unstructured mixture of {alpha}-helical and {beta}-sheet conformers in the solid state, the aggregates formed from the PrP 89-143 mutants appear to be mostly {beta}-sheet.

  9. The interactions of peripheral membrane proteins with biological membranes

    DOE PAGES [OSTI]

    Johs, Alexander; Whited, A. M.

    2015-01-01

    The interactions of peripheral proteins with membrane surfaces are critical to many biological processes, including signaling, recognition, membrane trafficking, cell division and cell structure. On a molecular level, peripheral membrane proteins can modulate lipid composition, membrane dynamics and protein-protein interactions. Biochemical and biophysical studies have shown that these interactions are in fact highly complex, dominated by several different types of interactions, and have an interdependent effect on both the protein and membrane. Here we examine three major mechanisms underlying the interactions between peripheral membrane proteins and membranes: electrostatic interactions, hydrophobic interactions, and fatty acid modification of proteins. While experimental approachesmore » continue to provide critical insights into specific interaction mechanisms, emerging bioinformatics resources and tools contribute to a systems-level picture of protein-lipid interactions. Through these recent advances, we begin to understand the pivotal role of protein-lipid interactions underlying complex biological functions at membrane interfaces.« less

  10. The interactions of peripheral membrane proteins with biological membranes

    SciTech Connect

    Johs, Alexander; Whited, A. M.

    2015-01-01

    The interactions of peripheral proteins with membrane surfaces are critical to many biological processes, including signaling, recognition, membrane trafficking, cell division and cell structure. On a molecular level, peripheral membrane proteins can modulate lipid composition, membrane dynamics and protein-protein interactions. Biochemical and biophysical studies have shown that these interactions are in fact highly complex, dominated by several different types of interactions, and have an interdependent effect on both the protein and membrane. Here we examine three major mechanisms underlying the interactions between peripheral membrane proteins and membranes: electrostatic interactions, hydrophobic interactions, and fatty acid modification of proteins. While experimental approaches continue to provide critical insights into specific interaction mechanisms, emerging bioinformatics resources and tools contribute to a systems-level picture of protein-lipid interactions. Through these recent advances, we begin to understand the pivotal role of protein-lipid interactions underlying complex biological functions at membrane interfaces.

  11. Biology Division. Progress report, August 1, 1982-September 30, 1983

    SciTech Connect

    Not Available

    1984-01-01

    The Biology Division is the component of the Oak Ridge National Laboratory that investigates the potential adverse health effects of energy-related substances. The body of this report provides summaries of the aims, scope and progress of the research of groups of investigators in the Division during the period of August 1, 1982, through September 30, 1983. At the end of each summary is a list of publications covering the same period (published or accepted for publication). For convenience, the summaries are assembled under Sections in accordance with the current organizational structure of the Biology Division; each Section begins with an overview. It will be apparent, however, that currents run throughout the Division and that the various programs support and interact with each other.

  12. STRUCtural Simulator

    Energy Science and Technology Software Center

    2004-07-01

    STRUC-ANL is a derivative of the FLUSTR-ANL finite element code. It contains only the structural capabilities of the original fluid-structural FLUSTR code.

  13. Structural Materials

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Structural Materials Development enables advanced technologies through the discovery, development, and demonstration of cost-effective advanced structural materials for use in ...

  14. Understanding of solar wind structure might be wrong

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Solar wind structure misunderstood Understanding of solar wind structure might be wrong The plasma particles flowing from the Sun and blasting past the Earth might be configured more as a network of tubes than a river-like stream. September 7, 2010 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy sources, to plasma physics and new

  15. A national facility for biological cryo-electron microscopy

    SciTech Connect

    Saibil, Helen R.; Grnewald, Kay; Stuart, David I.

    2015-01-01

    This review provides a brief update on the use of cryo-electron microscopy for integrated structural biology, along with an overview of the plans for the UK national facility for electron microscopy being built at the Diamond synchrotron. Three-dimensional electron microscopy is an enormously powerful tool for structural biologists. It is now able to provide an understanding of the molecular machinery of cells, disease processes and the actions of pathogenic organisms from atomic detail through to the cellular context. However, cutting-edge research in this field requires very substantial resources for equipment, infrastructure and expertise. Here, a brief overview is provided of the plans for a UK national three-dimensional electron-microscopy facility for integrated structural biology to enable internationally leading research on the machinery of life. State-of-the-art equipment operated with expert support will be provided, optimized for both atomic-level single-particle analysis of purified macromolecules and complexes and for tomography of cell sections. The access to and organization of the facility will be modelled on the highly successful macromolecular crystallography (MX) synchrotron beamlines, and will be embedded at the Diamond Light Source, facilitating the development of user-friendly workflows providing near-real-time experimental feedback.

  16. EUD-based biological optimization for carbon ion therapy

    SciTech Connect

    Brüningk, Sarah C. Kamp, Florian; Wilkens, Jan J.

    2015-11-15

    Purpose: Treatment planning for carbon ion therapy requires an accurate modeling of the biological response of each tissue to estimate the clinical outcome of a treatment. The relative biological effectiveness (RBE) accounts for this biological response on a cellular level but does not refer to the actual impact on the organ as a whole. For photon therapy, the concept of equivalent uniform dose (EUD) represents a simple model to take the organ response into account, yet so far no formulation of EUD has been reported that is suitable to carbon ion therapy. The authors introduce the concept of an equivalent uniform effect (EUE) that is directly applicable to both ion and photon therapies and exemplarily implemented it as a basis for biological treatment plan optimization for carbon ion therapy. Methods: In addition to a classical EUD concept, which calculates a generalized mean over the RBE-weighted dose distribution, the authors propose the EUE to simplify the optimization process of carbon ion therapy plans. The EUE is defined as the biologically equivalent uniform effect that yields the same probability of injury as the inhomogeneous effect distribution in an organ. Its mathematical formulation is based on the generalized mean effect using an effect-volume parameter to account for different organ architectures and is thus independent of a reference radiation. For both EUD concepts, quadratic and logistic objective functions are implemented into a research treatment planning system. A flexible implementation allows choosing for each structure between biological effect constraints per voxel and EUD constraints per structure. Exemplary treatment plans are calculated for a head-and-neck patient for multiple combinations of objective functions and optimization parameters. Results: Treatment plans optimized using an EUE-based objective function were comparable to those optimized with an RBE-weighted EUD-based approach. In agreement with previous results from photon

  17. Biological and chemical technologies research. FY 1995 annual summary report

    SciTech Connect

    1996-03-01

    The annual summary report presents the fiscal year (FY) 1995 research activities and accomplishments for the United States Department of Energy (DOE) Biological and Chemical Technologies Research (BCTR) Program. This BCTR program resides within the Office of Industrial Technologies (OIT) of the Office of Energy Efficiency and Renewable Energy (EE). The annual summary report for 1995 (ASR 95) contains the following: program description (including BCTR program mission statement, historical background, relevance, goals and objectives); program structure and organization, selected technical and programmatic highlights for 1995; detailed descriptions of individual projects; a listing of program output, including a bibliography of published work; patents; and awards arising from work supported by the BCTR.

  18. Method of measurement in biological systems

    DOEpatents

    Turteltaub, K.W.; Vogel, J.S.; Felton, J.S.; Gledhill, B.L.; Davis, J.C.

    1994-12-27

    Disclosed is a method of quantifying molecules in biological substances comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering the chemical specie to the biological host in doses sufficiently low to avoid significant overt damage to the biological system, d. allowing a period of time to elapse sufficient for dissemination and interaction of the chemical specie with the host throughout the biological system of the host, e. isolating a reacted fraction of the biological substance from the host in a manner sufficient to avoid contamination of the substance from extraneous sources, f. converting the fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in the material by means of direct isotopic counting. 5 figures.

  19. Method of measurement in biological systems

    DOEpatents

    Turteltaub, Kenneth W.; Vogel, John S.; Felton, James S.; Gledhill, Barton L.; Davis, Jay C.; Stanker, Larry H.

    1993-05-11

    Disclosed is a method of quantifying molecules in biological substances, comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering said chemical specie to said biological host in doses sufficiently low to avoid significant overt damage to the biological system thereof, d. allowing a period of time to elapse sufficient for dissemination and interaction of said chemical specie with said host throughout said biological system of said host, e. isolating a reacted fraction of the biological substance from said host in a manner sufficient to avoid contamination of said substance from extraneous sources, f. converting said fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in said material by means of direct isotopic counting.

  20. Method of measurement in biological systems

    DOEpatents

    Turteltaub, Kenneth W.; Vogel, John S.; Felton, James S.; Gledhill, Barton L.; Davis, Jay C.

    1994-01-01

    Disclosed is a method of quantifying molecules in biological substances comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering said chemical specie to said biological host in doses sufficiently low to avoid significant overt damage to the biological system thereof, d. allowing a period of time to elapse sufficient for dissemination and interaction of said chemical specie with said host throughout said biological system of said host, e. isolating a reacted fraction of the biological substance from said host in a manner sufficient to avoid contamination of said substance from extraneous sources, f. converting said fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in said material by means of direct isotopic counting.

  1. Catalyzing Inquiry at the Interface of Computing and Biology

    SciTech Connect

    John Wooley; Herbert S. Lin

    2005-10-30

    This study is the first comprehensive NRC study that suggests a high-level intellectual structure for Federal agencies for supporting work at the biology/computing interface. The report seeks to establish the intellectual legitimacy of a fundamentally cross-disciplinary collaboration between biologists and computer scientists. That is, while some universities are increasingly favorable to research at the intersection, life science researchers at other universities are strongly impeded in their efforts to collaborate. This report addresses these impediments and describes proven strategies for overcoming them. An important feature of the report is the use of well-documented examples that describe clearly to individuals not trained in computer science the value and usage of computing across the biological sciences, from genes and proteins to networks and pathways, from organelles to cells, and from individual organisms to populations and ecosystems. It is hoped that these examples will be useful to students in the life sciences to motivate (continued) study in computer science that will enable them to be more facile users of computing in their future biological studies.

  2. Grand Challenges for Biological and Environmental Research: A Long-Term Vision

    SciTech Connect

    Arkin, A.; Baliga, N.; Braam, J.; Church, G.; Collins, J; Cottingham, R.; Ecker, J.; Gerstein, M.; Gilna, P.; Greenberg, J.; Handelsman, J.; Hubbard, S.; Joachimiak, A.; Liao, J.; Looger, L.; Meyerowitz, E.; Mjolness, E.; Petsko, G.; Sayler, G.; Simpson, M.; Stacey, G.; Sussman, M.; Tiedje, J.; Bader, D.; Cessi, P.; Collins, W.; Denning, S.; Dickinson, R.; Easterling, D.; Edmonds, J.; Feddema, J.; Field, C.; Fridlind, A.; Fung, I.; Held, I.; Jackson, R.; Janetos, A.; Large, W.; Leinen, M.; Leung, R.; Long, S.; Mace, G.; Masiello, C.; Meehl, G.; Ort, D.; Otto-Bliesner, B.; Penner, J.; Prather, M.; Randall, D.; Rasch, P.; Schneider, E.; Shugart, H.; Thornton, P.; Washington, W.; Wildung, R.; Wiscombe, W.; Zak, D.; Zhang, M.; Bielicki, J.; Buford, M.; Cleland, E.; Dale, V.; Duke, C.; Ehleringer, J.; Hecht, A.; Kammen, D.; Marland, G.; Pataki, D.; Riley, M. Robertson, P.; Hubbard, S.

    2010-12-01

    The interactions and feedbacks among plants, animals, microbes, humans, and the environment ultimately form the world in which we live. This world is now facing challenges from a growing and increasingly affluent human population whose numbers and lifestyles are driving ever greater energy demand and impacting climate. These and other contributing factors will make energy and climate sustainability extremely difficult to achieve over the 20-year time horizon that is the focus of this report. Despite these severe challenges, there is optimism that deeper understanding of our environment will enable us to mitigate detrimental effects, while also harnessing biological and climate systems to ensure a sustainable energy future. This effort is advanced by scientific inquiries in the fields of atmospheric chemistry and physics, biology, ecology, and subsurface science - all made possible by computing. The Office of Biological and Environmental Research (BER) within the Department of Energy's (DOE) Office of Science has a long history of bringing together researchers from different disciplines to address critical national needs in determining the biological and environmental impacts of energy production and use, characterizing the interplay of climate and energy, and collaborating with other agencies and DOE programs to improve the world's most powerful climate models. BER science focuses on three distinct areas: (1) What are the roles of Earth system components (atmosphere, land, oceans, sea ice, and the biosphere) in determining climate? (2) How is the information stored in a genome translated into microbial, plant, and ecosystem processes that influence biofuel production, climate feedbacks, and the natural cycling of carbon? (3) What are the biological, geochemical, and physical forces that govern the behavior of Earth's subsurface environment? Ultimately, the goal of BER science is to support experimentation and modeling that can reliably predict the outcomes and

  3. Full-length G[alpha subscript q]–phospholipase C-[beta]3 structure...

    Office of Scientific and Technical Information (OSTI)

    Resource Type: Journal Article Resource Relation: Journal Name: Nature Structural & Molecular Biology; Journal Volume: 20; Journal Issue: (3) ; 03, 2013 Publisher: Nature ...

  4. Sequence-dependent structural changes in a self-assembling DNA...

    Office of Scientific and Technical Information (OSTI)

    Resource Type: Journal Article Resource Relation: Journal Name: Acta Crystallographica. Section D. Structural Biology; Journal Volume: 71; Journal Issue: (12) ; 12, 2015 Publisher: ...

  5. Structure of the human MLH1 N-terminus: implications for predispositio...

    Office of Scientific and Technical Information (OSTI)

    Article Resource Relation: Journal Name: Acta crystallographica. Section F, Structural biology communications; Journal Volume: 71; Journal Issue: Pt 8; Other Information: PMCID:...

  6. Crystal structure of N-{N-[N-acetyl-(S)-leucyl]-(S)-leucyl}norleucinal...

    Office of Scientific and Technical Information (OSTI)

    N and O atoms in intermolecular hydrogen bonds forming an infinite antiparallel -sheet. ... Subject: 59 BASIC BIOLOGICAL SCIENCES crystal structure; proteasome inhibitor; hydrogen ...

  7. Algal Biology Program at Los Alamos

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Algal Biology Program at Los Alamos gets a star October 11, 2011 LOS ALAMOS, New Mexico, October 11, 2011-Richard Sayre, one of the nation's top specialists in algae and...

  8. Renewable Hydrogen Production from Biological Systems

    Office of Energy Efficiency and Renewable Energy (EERE)

    Presentation by Matthew Posewitz, Colorado School of Mines, at the Biological Hydrogen Production Workshop held September 24-25, 2013, at the National Renewable Energy Laboratory in Golden, Colorado.

  9. Biological denitrification of high concentration nitrate waste

    DOEpatents

    Francis, Chester W.; Brinkley, Frank S.

    1977-01-01

    Biological denitrification of nitrate solutions at concentrations of greater than one kilogram nitrate per cubic meter is accomplished anaerobically in an upflow column having as a packing material a support for denitrifying bacteria.

  10. Genetics and molecular biology of breast cancer

    SciTech Connect

    King, M.C.; Lippman, M.

    1992-12-31

    This volume contains the abstracts of oral presentations and poster sessions presented at the Cold Springs Harbor Meeting on Cancer Cells, this meeting entitled Genetics and Molecular Biology of Breast Cancer.

  11. BCTR: Biological and Chemical Technologies Research 1994 annual summary report

    SciTech Connect

    Petersen, G.

    1995-02-01

    The annual summary report presents the fiscal year (FY) 1994 research activities and accomplishments for the United States Department of Energy (DOE) Biological and Chemical Technologies Research (BCTR) Program of the Advanced Industrial Concepts Division (AICD). This AICD program resides within the Office of Industrial Technologies (OIT) of the Office of Energy Efficiency and Renewable Energy (EE). Although the OIT was reorganized in 1991 and AICD no longer exists, this document reports on efforts conducted under the former structure. The annual summary report for 1994 (ASR 94) contains the following: program description (including BCTR program mission statement, historical background, relevance, goals and objectives); program structure and organization, selected technical and programmatic highlights for 1994; detailed descriptions of individual projects; a listing of program output, including a bibliography of published work; patents, and awards arising from work supported by BCTR.

  12. Biological and Environmental Research Advisory Committee

    Office of Science (SC)

    February 26-27, 2015, Tysons, Virginia | U.S. DOE Office of Science (SC) February 26-27, 2015, Tysons, Virginia Biological and Environmental Research Advisory Committee (BERAC) BERAC Home Meetings BERAC Minutes BERAC Minutes Archive Members Charges/Reports Charter .pdf file (135KB) BER Committees of Visitors Federal Advisory Committees BER Home Meetings Biological and Environmental Research Advisory Committee February 26-27, 2015, Tysons, Virginia Print Text Size: A A A FeedbackShare Page

  13. Biological and Environmental Research Advisory Committee

    Office of Science (SC)

    October 28-29, 2013 | U.S. DOE Office of Science (SC) October 28-29, 2013 Biological and Environmental Research Advisory Committee (BERAC) BERAC Home Meetings BERAC Minutes BERAC Minutes Archive Members Charges/Reports Charter .pdf file (135KB) BER Committees of Visitors Federal Advisory Committees BER Home Meetings Biological and Environmental Research Advisory Committee October 28-29, 2013 Print Text Size: A A A FeedbackShare Page Agenda .pdf file (10KB) Presentations: Sharlene Weatherwax

  14. Sealable femtoliter chamber arrays for cell-free biology

    DOE PAGES [OSTI]

    Retterer, Scott T.; Fowlkes, Jason Davidson; Collier, Charles Patrick; Simpson, Michael L.; Norred, Sarah Elizabeth; Caveney, Patrick M.; Boreyko, Jonathan B.

    2015-03-11

    Cell-free systems provide a flexible platform for probing specific networks of biological reactions isolated from the complex resource sharing (e.g. global gene expression, cell division) encountered within living cells. However, such systems, used in conventional macro-scale bulk reactors, often fail to exhibit the dynamic behaviors and efficiencies characteristic of their living micro-scale counterparts. Understanding the impact of internal cell structure and scale on reaction dynamics is crucial to understanding complex gene networks. Here we report a microfabricated device that confines cell-free reactions in cellular scale volumes while allowing flexible characterization of the enclosed molecular system. This multilayered poly(dimethylsiloxane) (PDMS) devicemore » contains femtoliter-scale reaction chambers on an elastomeric membrane which can be actuated (open and closed). When actuated, the chambers confine Cell-Free Protein Synthesis (CFPS) reactions expressing a fluorescent protein, allowing for the visualization of the reaction kinetics over time using time-lapse fluorescent microscopy. Lastly, we demonstrate how this device may be used to measure the noise structure of CFPS reactions in a manner that is directly analogous to those used to characterize cellular systems, thereby enabling the use of noise biology techniques to characterize CFPS gene circuits and their interactions with the cell-free environment.« less

  15. Uncovering biologically significant lipid isomers with liquid chromatography, ion mobility spectrometry and mass spectrometry

    SciTech Connect

    Kyle, Jennifer E.; Zhang, Xing; Weitz, Karl K.; Monroe, Matthew E.; Ibrahim, Yehia M.; Moore, Ronald J.; Cha, Jeeyeon; Sun, Xiaofei; Lovelace, Erica S.; Wagoner, Jessica; Polyak, Stephen J.; Metz, Thomas O.; Dey, Sudhansu K.; Smith, Richard D.; Burnum-Johnson, Kristin E.; Baker, Erin S.

    2016-01-01

    Understanding how biological molecules are generated, metabolized and eliminated in living systems is important for interpreting processes such as immune response and disease pathology. While genomic and proteomic studies have provided vast amounts of information over the last several decades, interest in lipidomics has also grown due to improved analytical technologies revealing altered lipid metabolism in type 2 diabetes, cancer, and lipid storage disease. Liquid chromatography and mass spectrometry (LC-MS) measurements are currently the dominant approach for characterizing the lipidome by providing detailed information on the spatial and temporal composition of lipids. However, interpreting lipids’ biological roles is challenging due to the existence of numerous structural and stereoisomers (i.e. distinct acyl chain and double-bond positions), which are unresolvable using present LC-MS approaches. Here we show that combining structurally-based ion mobility spectrometry (IMS) with LC-MS measurements distinguishes lipid isomers and allows insight into biological and disease processes.

  16. UK Biotechnology and Biological Sciences Research Council | Open...

    OpenEI (Open Energy Information) [EERE & EIA]

    Biotechnology and Biological Sciences Research Council Jump to: navigation, search Name: UK Biotechnology and Biological Sciences Research Council Place: London, United Kingdom...

  17. Techno-Economic Boundary Analysis of Biological Pathways to Hydrogen...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Techno-Economic Boundary Analysis of Biological Pathways to Hydrogen Production (2009) Techno-Economic Boundary Analysis of Biological Pathways to Hydrogen Production (2009) ...

  18. Kahuku Wind Power Biological Opinion | Department of Energy

    Energy Saver

    Kahuku Wind Power Biological Opinion Kahuku Wind Power Biological Opinion Kahuku Wind Power, LLC, Construction of the Kahuku Wind Power Facility in Kahuku, O'ahu, Hawaii Kahuku ...

  19. Algal Biology Toolbox Workshop Brings Lead Experts to Inform...

    Office of Environmental Management (EM)

    Algal Biology Toolbox Workshop Brings Lead Experts to Inform Algae-Based Biofuel Strategy Algal Biology Toolbox Workshop Brings Lead Experts to Inform Algae-Based Biofuel Strategy ...

  20. EERE Success Story-California: Breakthrough in Algae Biology...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Breakthrough in Algae Biology EERE Success Story-California: Breakthrough in Algae Biology January 31, 2014 - 12:00am Addthis Researchers at the Scripps Institution of Oceanography ...

  1. Solar Energy Education. Renewable energy activities for biology...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    biology Citation Details In-Document Search Title: Solar Energy Education. Renewable energy activities for biology You are accessing a document from the Department of Energy's ...

  2. Flow cytometry aids basic cell biology research and drug discovery

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Flow cytometry aids basic cell biology research and drug discovery Flow cytometry aids basic cell biology research and drug discovery Life Technologies Corporation and LANL have ...

  3. DOE Publishes Roadmap for New Biological Research for Energy...

    Office of Science (SC)

    plan for a new generation of biology research that builds on genome project ... The Genomics: GTL Roadmap: Systems Biology for Energy and Environment outlines a plan to ...

  4. Nanojunction Sensors for the Detection of chemical and Biological...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Nanojunction Sensors for the Detection of chemical and Biological Species DOE Grant ... gaps for various applications, including chemical and biological sensors, magnetoresistive ...

  5. Detter, John C. [Los Alamos National Laboratory] Basic Biological

    Office of Scientific and Technical Information (OSTI)

    State of the Art for Autonomous Detection Systems using Genomic Sequencing Detter, John C. Los Alamos National Laboratory Basic Biological Sciences(59) Biological Science...

  6. Hydro Review: Computational Tools to Assess Turbine Biological...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Hydro Review: Computational Tools to Assess Turbine Biological Performance Hydro Review: Computational Tools to Assess Turbine Biological Performance This review covers the BioPA ...

  7. Copy of Synthetic Biology of Novel Thermophilic Bacteria for...

    Office of Scientific and Technical Information (OSTI)

    Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of ... Title: Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of ...

  8. Comparative systems biology across an evolutionary gradient within...

    Office of Scientific and Technical Information (OSTI)

    Comparative systems biology across an evolutionary gradient within the Shewanella genus Citation Details In-Document Search Title: Comparative systems biology across an ...

  9. Systems biology analysis of Zymomonas mobilis ZM4 ethanol stress...

    Office of Scientific and Technical Information (OSTI)

    Systems biology analysis of Zymomonas mobilis ZM4 ethanol stress responses Citation Details In-Document Search Title: Systems biology analysis of Zymomonas mobilis ZM4 ethanol ...

  10. Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen Production Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen Production Report documenting the ...

  11. Synthetic Biology for Advanced Fuels (Opening Keynote Address...

    Office of Scientific and Technical Information (OSTI)

    Synthetic Biology for Advanced Fuels (Opening Keynote Address - 2010 JGI User Meeting) Citation Details In-Document Search Title: Synthetic Biology for Advanced Fuels (Opening ...

  12. Genetics and Molecular Biology of Hydrogen Metabolism in Sulfate...

    Office of Scientific and Technical Information (OSTI)

    Genetics and Molecular Biology of Hydrogen Metabolism in Sulfate-Reducing Bacteria Citation Details In-Document Search Title: Genetics and Molecular Biology of Hydrogen Metabolism ...

  13. California: Breakthrough in Algae Biology | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Breakthrough in Algae Biology California: Breakthrough in Algae Biology January 31, 2014 - 12:00am Addthis Researchers at the Scripps Institution of Oceanography at University of ...

  14. Sealable femtoliter chamber arrays for cell-free biology (Journal...

    Office of Scientific and Technical Information (OSTI)

    Sealable femtoliter chamber arrays for cell-free biology Prev Next Title: Sealable femtoliter chamber arrays for cell-free biology You are accessing a document from the ...

  15. Importance of systems biology in engineering microbes for biofuel...

    Office of Scientific and Technical Information (OSTI)

    Importance of systems biology in engineering microbes for biofuel production Citation Details In-Document Search Title: Importance of systems biology in engineering microbes for ...

  16. Biology and Medicine Division annual report, 1981-1982. [Lead abstract

    SciTech Connect

    Not Available

    1983-04-01

    Separate abstracts were prepared for the 61 research reports in the 1981-1982 annual report for the Biology and Medicine Division of the Lawrence Berkeley Laboratory. Programs reviewed include research medicine, Donner Pavilion, environmental physiology, radiation biophysics and structural biophysics. (KRM)

  17. Thermolysis of partial coal structures: bibenzyl and naphthols

    SciTech Connect

    Poutsma, M L

    1980-01-01

    All proposed processes for direct conversion of coal to synthetic liquid fuels require heating to greater than or equal to 400/sup 0/C. Exact process conditions (pyrolysis, hydropyrolysis, catalytic hydrogenation, donor solvent refining) are vital in determining the final product state (1). However, underlying all these processes may well be a set of similar thermal reactions which lead to disruption of the organic macromolecular structure of coal. Because of the structural complexity and irregularity of coal, attempts to elucidate this thermal chemistry of coal and of donor process solvents have relied heavily on thermolysis studies of model organic structures (2) and the judicious use of structure-reactivity relationships which are central to the discipline of physical organic chemistry. In this work the reaction products of the pyrolysis of bibenzyl and naphthals were identified.

  18. Cement-aggregate compatibility and structure property relationships including modelling

    SciTech Connect

    Jennings, H.M.; Xi, Y.

    1993-07-15

    The role of aggregate, and its interface with cement paste, is discussed with a view toward establishing models that relate structure to properties. Both short (nm) and long (mm) range structure must be considered. The short range structure of the interface depends not only on the physical distribution of the various phases, but also on moisture content and reactivity of aggregate. Changes that occur on drying, i.e. shrinkage, may alter the structure which, in turn, feeds back to alter further drying and shrinkage. The interaction is dynamic, even without further hydration of cement paste, and the dynamic characteristic must be considered in order to fully understand and model its contribution to properties. Microstructure and properties are two subjects which have been pursued somewhat separately. This review discusses both disciplines with a view toward finding common research goals in the future. Finally, comment is made on possible chemical reactions which may occur between aggregate and cement paste.

  19. Structural Materials

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Structural Materials Structural Materials Development enables advanced technologies through the discovery, development, and demonstration of cost-effective advanced structural materials for use in extreme environments (high-temperature, high-stress, erosive, and corrosive environments, including the performance of materials in contact with molten slags and salts). Research includes materials design and discovery, materials processing and manufacturing, and service-life prediction of materials

  20. Department of Energy's Biological and Environmental Research Strategic Data Roadmap for Earth System Science

    SciTech Connect

    Williams, Dean N.; Palanisamy, Giri; Shipman, Galen; Boden, Thomas A.; Voyles, Jimmy W.

    2014-04-25

    Rapid advances in experimental, sensor, and computational technologies and techniques are driving exponential growth in the volume, acquisition rate, variety, and complexity of scientific data. This wealth of scientifically meaningful data has tremendous potential to lead to scientific discovery. However, to achieve scientific breakthroughs, these data must be exploitable—they must be analyzed effectively and efficiently and the results shared and communicated easily within the wider Department of Energy’s (DOE’s) Biological and Environmental Research (BER) Climate and Environmental Sciences Division (CESD) community. The explosion in data complexity and scale makes these tasks exceedingly difficult to achieve, particularly given that an increasing number of disciplines are working across techniques, integrating simulation and experimental or observational results (see Table 5 in Appendix 2). Consequently, we need new approaches to data management, analysis, and visualization that provide research teams with easy-to-use and scalable end-to-end solutions. These solutions must facilitate (and where feasible, automate and capture) every stage in the data lifecycle (shown in Figure 1), from collection to management, annotation, sharing, discovery, analysis, and visualization. In addition, the core functionalities are the same across climate science communities, but they require customization to adapt to specific needs and fit into research and analysis workflows. To this end, the mission of CESD’s Data and Informatics Program is to integrate all existing and future distributed CESD data holdings into a seamless and unified environment for the acceleration of Earth system science.

  1. The Intersection of Physics and Biology

    ScienceCinema

    Liphardt, Jan [University of California, Berkeley, California, United States

    2016-07-12

    In April 1953, Watson and Crick largely defined the program of 20th century biology: obtaining the blueprint of life encoded in the DNA. Fifty years later, in 2003, the sequencing of the human genome was completed. Like any major scientific breakthrough, the sequencing of the human genome raised many more questions than it answered. I'll brief you on some of the big open problems in cell and developmental biology, and I'll explain why approaches, tools, and ideas from the physical sciences are currently reshaping biological research. Super-resolution light microscopies are revealing the intricate spatial organization of cells, single-molecule methods show how molecular machines function, and new probes are clarifying the role of mechanical forces in cell and tissue function. At the same time, Physics stands to gain beautiful new problems in soft condensed matter, quantum mechanics, and non-equilibrium thermodynamics.

  2. Los Alamos structure-damage software wins R&D 100 Award

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Structure-damage software wins R&D 100 Award Los Alamos structure-damage software wins R&D 100 Award A software package called SHMTools, which can detect damage in a variety of structures and was developed at Los Alamos National Laboratory, won an R&D 100 Award. December 6, 2015 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience,

  3. The acquisition of dangerous biological materials :

    SciTech Connect

    Aceto, Donato Gonzalo; Astuto-Gribble, Lisa M.; Gaudioso, Jennifer M.

    2007-11-01

    Numerous terrorist organizations have openly expressed interest in producing and deploying biological weapons. However, a limiting factor for many terrorists has been the acquisition of dangerous biological agents, as evidenced by the very few successful instances of biological weapons use compared to the number of documented hoaxes. Biological agents vary greatly in their ability to cause loss of life and economic damage. Some agents, if released properly, can kill many people and cause an extensive number of secondary infections; other agents will sicken only a small number of people for a short period of time. Consequently, several biological agents can potentially be used to perpetrate a bioterrorism attack but few are likely capable of causing a high consequence event. It is crucial, from a US national security perspective, to more deeply understand the likelihood that terrorist organizations can acquire the range of these agents. Few studies have attempted to comprehensively compile the technical information directly relevant to the acquisition of dangerous bacteria, viruses and toxins. In this report, technical fact sheets were assembled for 46 potentially dangerous biological agents. Much of the information was taken from various research sources which could ultimately and significantly expedite and improve bioterrorism threat assessments. By systematically examining a number of specific agent characteristics included in these fact sheets, it may be possible to detect, target, and implement measures to thwart future terrorist acquisition attempts. In addition, the information in these fact sheets may be used as a tool to help laboratories gain a rudimentary understanding of how attractive a method laboratory theft is relative to other potential acquisition modes.

  4. Biological cell classification by multiangle light scattering

    DOEpatents

    Salzman, G.C.; Crowell, J.M.; Mullaney, P.F.

    1975-06-03

    The specification is directed to an apparatus and method for detecting light scattering from a biological cell. Light, preferably from a coherent source of radiation, intercepts an individual biological cell in a stream of cells passing through the beam. Light scattered from the cell is detected at a selected number of angles between 0 and 90/sup 0/ to the longitudinal axis of the beam with a circular array of light responsive elements which produce signals representative of the intensity of light incident thereon. Signals from the elements are processed to determine the light-scattering pattern of the cell and therefrom its identity.

  5. Biological and Environmental Research Advisory Committee

    Office of Science (SC)

    March 03-04, 2014 | U.S. DOE Office of Science (SC) March 03-04, 2014 Biological and Environmental Research Advisory Committee (BERAC) BERAC Home Meetings BERAC Minutes BERAC Minutes Archive Members Charges/Reports Charter .pdf file (135KB) BER Committees of Visitors Federal Advisory Committees BER Home Meetings Biological and Environmental Research Advisory Committee March 03-04, 2014 Print Text Size: A A A FeedbackShare Page Agenda .pdf file (21KB) Presentations: Sharlene Weatherwax .pdf

  6. Biological and Environmental Research Advisory Committee

    Office of Science (SC)

    October 1-2, 2014, Rockville, MD | U.S. DOE Office of Science (SC) October 1-2, 2014, Rockville, MD Biological and Environmental Research Advisory Committee (BERAC) BERAC Home Meetings BERAC Minutes BERAC Minutes Archive Members Charges/Reports Charter .pdf file (135KB) BER Committees of Visitors Federal Advisory Committees BER Home Meetings Biological and Environmental Research Advisory Committee October 1-2, 2014, Rockville, MD Print Text Size: A A A FeedbackShare Page Agenda .pdf file

  7. Spectroscopic diagnostics for bacteria in biologic sample

    DOEpatents

    El-Sayed, Mostafa A.; El-Sayed, Ivan H.

    2002-01-01

    A method to analyze and diagnose specific bacteria in a biologic sample using spectroscopy is disclosed. The method includes obtaining the spectra of a biologic sample of a non-infected patient for use as a reference, subtracting the reference from the spectra of an infected sample, and comparing the fingerprint regions of the resulting differential spectrum with reference spectra of bacteria in saline. Using this diagnostic technique, specific bacteria can be identified sooner and without culturing, bacteria-specific antibiotics can be prescribed sooner, resulting in decreased likelihood of antibiotic resistance and an overall reduction of medical costs.

  8. Autofermentative Biological Hydrogen Production by Cyanobacteria

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    BioSolarH 2  Autofermentative biological hydrogen production by cyanobacteria G.C. Dismukes Rutgers University Waksman Institute and Department of Chemistry & Chemical Biology DOE Biohydrogen Production Workshop NREL October, 2013 -BioSolarH 2  Ghirardi et al., 2007 Tamagnini et al., 2007 Soluble NiFe hydrogenase (SH) Group 5 AH in Ralstonia eutropha H16 Schäfer et al., 2013 Formate dehydrogenase Hydrogenase Bagramyan et al., 2003 Ferredoxin Km (MV) = 16.1µM Kcat (MV) = 1242 s -1

  9. Biology and Medicine Division: Annual report 1986

    SciTech Connect

    Not Available

    1987-04-01

    The Biology and Medicine Division continues to make important contributions in scientific areas in which it has a long-established leadership role. For 50 years the Division has pioneered in the application of radioisotopes and charged particles to biology and medicine. There is a growing emphasis on cellular and molecular applications in the work of all the Division's research groups. The powerful tools of genetic engineering, the use of recombinant products, the analytical application of DNA probes, and the use of restriction fragment length polymorphic DNA are described and proposed for increasing use in the future.

  10. EERE Success Story—California: Breakthrough in Algae Biology

    Office of Energy Efficiency and Renewable Energy (EERE)

    Breakthrough in algae biology will have a significant impact in the economics of algal biofuel production.

  11. Biological Conversion of Sugars To Hydrocarbons | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    To Hydrocarbons Biological Conversion of Sugars To Hydrocarbons PDF explaining the biological process of bioenergy Biological Conversion of Sugars To Hydrocarbons (190.69 KB) More Documents & Publications Catalytic Upgrading Sugars To Hydrocarbons Catalytic Upgrading of Sugars to Hydrocarbons Technology Pathway Biological Conversion of Sugars to Hydrocarbons Technology Pathway

  12. Modular microfluidic system for biological sample preparation

    DOEpatents

    Rose, Klint A.; Mariella, Jr., Raymond P.; Bailey, Christopher G.; Ness, Kevin Dean

    2015-09-29

    A reconfigurable modular microfluidic system for preparation of a biological sample including a series of reconfigurable modules for automated sample preparation adapted to selectively include a) a microfluidic acoustic focusing filter module, b) a dielectrophoresis bacteria filter module, c) a dielectrophoresis virus filter module, d) an isotachophoresis nucleic acid filter module, e) a lyses module, and f) an isotachophoresis-based nucleic acid filter.

  13. Composites comprising biologically-synthesized nanomaterials

    DOEpatents

    Curran, Seamus; Dias, Sampath; Blau, Werner; Wang, Jun; Oremland, Ronald S; Baesman, Shaun

    2013-04-30

    The present disclosure describes composite materials containing a polymer material and a nanoscale material dispersed in the polymer material. The nanoscale materials may be biologically synthesized, such as tellurium nanorods synthesized by Bacillus selenitireducens. Composite materials of the present disclosure may have optical limiting properties and find use in optical limiting devices.

  14. Radiological/biological/aerosol removal system

    DOEpatents

    Haslam, Jeffery J

    2015-03-17

    An air filter replacement system for existing buildings, vehicles, arenas, and other enclosed airspaces includes a replacement air filter for replacing a standard air filter. The replacement air filter has dimensions and air flow specifications that allow it to replace the standard air filter. The replacement air filter includes a filter material that removes radiological or biological or aerosol particles.

  15. Biological effectiveness of neutrons: Research needs

    SciTech Connect

    Casarett, G.W.; Braby, L.A.; Broerse, J.J.; Elkind, M.M.; Goodhead, D.T.; Oleinick, N.L.

    1994-02-01

    The goal of this report was to provide a conceptual plan for a research program that would provide a basis for determining more precisely the biological effectiveness of neutron radiation with emphasis on endpoints relevant to the protection of human health. This report presents the findings of the experts for seven particular categories of scientific information on neutron biological effectiveness. Chapter 2 examines the radiobiological mechanisms underlying the assumptions used to estimate human risk from neutrons and other radiations. Chapter 3 discusses the qualitative and quantitative models used to organize and evaluate experimental observations and to provide extrapolations where direct observations cannot be made. Chapter 4 discusses the physical principles governing the interaction of radiation with biological systems and the importance of accurate dosimetry in evaluating radiation risk and reducing the uncertainty in the biological data. Chapter 5 deals with the chemical and molecular changes underlying cellular responses and the LET dependence of these changes. Chapter 6, in turn, discusses those cellular and genetic changes which lead to mutation or neoplastic transformation. Chapters 7 and 8 examine deterministic and stochastic effects, respectively, and the data required for the prediction of such effects at different organizational levels and for the extrapolation from experimental results in animals to risks for man. Gaps and uncertainties in this data are examined relative to data required for establishing radiation protection standards for neutrons and procedures for the effective and safe use of neutron and other high-LET radiation therapy.

  16. Apparatus for automated testing of biological specimens

    DOEpatents

    Layne, Scott P.; Beugelsdijk, Tony J.

    1999-01-01

    An apparatus for performing automated testing of infections biological specimens is disclosed. The apparatus comprise a process controller for translating user commands into test instrument suite commands, and a test instrument suite comprising a means to treat the specimen to manifest an observable result, and a detector for measuring the observable result to generate specimen test results.

  17. Method and apparatus for sustaining viability of biological cells on a substrate

    DOEpatents

    McKnight, Timothy E.; Melechko, Anatoli V.; Simpson, Michael L.

    2013-01-01

    A method for the transient transformation of a living biological cell having an intact cell membrane defining an intracellular domain, and an apparatus for the transient transformation of biological cells. The method and apparatus include introducing a compartmentalized extracellular component fixedly attached to a cellular penetrant structure to the intracellular domain of the cell, wherein the cell is fixed in a predetermined location and wherein the component is expressed within in the cell while being retained within the compartment and wherein the compartment restricts the mobility and interactions of the component within the cell and prevents transference of the component to the cell.

  18. Method and apparatus for sustaining viability of biological cells on a substrate

    DOEpatents

    McKnight, Timothy E. (Greenback, TN); Melechko, Anatoli V. (Oak Ridge, TN); Simpson, Michael L. (Knoxville, TN)

    2011-12-13

    A method for the transient transformation of a living biological cell having an intact cell membrane defining an intracellular domain, and an apparatus for the transient transformation of biological cells. The method and apparatus include introducing a compartmentalized extracellular component fixedly attached to a cellular penetrant structure to the intracellular domain of the cell, wherein the cell is fixed in a predetermined location and wherein the component is expressed within in the cell while being retained within the compartment and wherein the compartment restricts the mobility and interactions of the component within the cell and prevents transference of the component to the cell.

  19. Biological imaging by soft x-ray diffraction microscopy

    DOE PAGES [OSTI]

    Shapiro, D.; Thibault, P.; Beetz, T.; Elser, V.; Howells, M.; Jacobsen, C.; Kirz, J.; Lima, E.; Miao, H.; Neiman, A. M.; et al

    2005-10-25

    We have used the method of x-ray diffraction microscopy to image the complex-valued exit wave of an intact and unstained yeast cell. The images of the freeze-dried cell, obtained by using 750-eV x-rays from different angular orientations, portray several of the cell's major internal components to 30-nm resolution. The good agreement among the independently recovered structures demonstrates the accuracy of the imaging technique. To obtain the best possible reconstructions, we have implemented procedures for handling noisy and incomplete diffraction data, and we propose a method for determining the reconstructed resolution. This work represents a previously uncharacterized application of x-ray diffractionmore » microscopy to a specimen of this complexity and provides confidence in the feasibility of the ultimate goal of imaging biological specimens at 10-nm resolution in three dimensions.« less

  20. Biology Division progress report, October 1, 1984-September 30, 1985

    SciTech Connect

    Not Available

    1986-01-01

    The body of this report provides summaries of the aims, scope and progress of the research by groups of investigators in the Division during the period of October 1, 1984, through September 30, 1985. At the end of each summary is a list of publications covering the same period. For convenience, the summaries are assembled under Sections in accordance with the current organizational structure of the Biology Division; each Section begins with an overview. It will be apparent, however, tha crosscurrents run throughout the Division and that the various programs support and interact with each other. In addition, this report includes information on the Division's educational activities, Advisory Committee, seminar program, and international interactions, as well as extramural activities of staff members, abstracts for technical meetings, and funding and personnel levels.

  1. Interaction mechanisms and biological effects of static magnetic fields

    SciTech Connect

    Tenforde, T.S.

    1994-06-01

    Mechanisms through which static magnetic fields interact with living systems are described and illustrated by selected experimental observations. These mechanisms include electrodynamic interactions with moving, ionic charges (blood flow and nerve impulse conduction), magnetomechanical interactions (orientation and translation of molecules structures and magnetic particles), and interactions with electronic spin states in charge transfer reactions (photo-induced electron transfer in photosynthesis). A general summary is also presented of the biological effects of static magnetic fields. There is convincing experimental evidence for magnetoreception mechanisms in several classes of lower organisms, including bacteria and marine organisms. However, in more highly evolved species of animals, there is no evidence that the interactions of static magnetic fields with flux densities up to 2 Tesla (1 Tesla [T] = 10{sup 4} Gauss) produce either behavioral or physiolocical alterations. These results, based on controlled studies with laboratory animals, are consistent with the outcome of recent epidemiological surveys on human populations exposed occupationally to static magnetic fields.

  2. Work Force Discipline--Withdrawn

    Directives, Delegations, and Other Requirements [Office of Management (MA)]

    2012-04-05

    Withdrawn 4-26-14. Significant changes from the Order include the deletion of material included in other documents such as statutes and Federal regulations. In addition, an updated Table of Penalties now includes Smoking, Cyber Security, Standards of Conduct or other Ethical Violations, and the incorporation of the Travel and Purchase Card Guidance dated January 2003.

  3. Organizational Structure | Photosynthetic Antenna Research Center

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Organizational Structure Organizational Structure

  4. Synthetic fossilization of soft biological tissues and their shape-preserving transformation into silica or electron-conductive replicas

    DOE PAGES [OSTI]

    Townson, Jason L.; Lin, Yu-Shen; Chou, Stanley S.; Awad, Yasmine H.; Coker, Eric N.; Brinker, C. Jeffrey; Kaehr, Bryan

    2014-12-08

    Structural preservation of complex biological systems from the subcellular to whole organism level in robust forms, enabling dissection and imaging while preserving 3D context, represents an enduring grand challenge in biology. Here we report a simple immersion method for structurally preserving intact organisms via conformal stabilization within silica. This self-limiting process, which we refer to as silica bioreplication, occurs by condensation of water-soluble silicic acid proximally to biomolecular interfaces throughout the organism. Conformal nanoscopic silicification of all biomolecular features imparts structural rigidity enabling the preservation of shape and nano-to-macroscale dimensional features upon drying to form a biocomposite and further highmore » temperature oxidative calcination to form silica replicas or reductive pyrolysis to form electrically conductive carbon replicas of complete organisms. Ultimately, the simplicity and generalizability of this approach should facilitate efforts in biological preservation and analysis and could enable the development of new classes of biomimetic composite materials.« less

  5. Synthetic fossilization of soft biological tissues and their shape-preserving transformation into silica or electron-conductive replicas

    SciTech Connect

    Townson, Jason L.; Lin, Yu-Shen; Chou, Stanley S.; Awad, Yasmine H.; Coker, Eric N.; Brinker, C. Jeffrey; Kaehr, Bryan

    2014-12-08

    Structural preservation of complex biological systems from the subcellular to whole organism level in robust forms, enabling dissection and imaging while preserving 3D context, represents an enduring grand challenge in biology. Here we report a simple immersion method for structurally preserving intact organisms via conformal stabilization within silica. This self-limiting process, which we refer to as silica bioreplication, occurs by condensation of water-soluble silicic acid proximally to biomolecular interfaces throughout the organism. Conformal nanoscopic silicification of all biomolecular features imparts structural rigidity enabling the preservation of shape and nano-to-macroscale dimensional features upon drying to form a biocomposite and further high temperature oxidative calcination to form silica replicas or reductive pyrolysis to form electrically conductive carbon replicas of complete organisms. Ultimately, the simplicity and generalizability of this approach should facilitate efforts in biological preservation and analysis and could enable the development of new classes of biomimetic composite materials.

  6. Monitoring Biological Activity at Geothermal Power Plants

    SciTech Connect

    Peter Pryfogle

    2005-09-01

    The economic impact of microbial growth in geothermal power plants has been estimated to be as high as $500,000 annually for a 100 MWe plant. Many methods are available to monitor biological activity at these facilities; however, very few plants have any on-line monitoring program in place. Metal coupon, selective culturing (MPN), total organic carbon (TOC), adenosine triphosphate (ATP), respirometry, phospholipid fatty acid (PLFA), and denaturing gradient gel electrophoresis (DGGE) characterizations have been conducted using water samples collected from geothermal plants located in California and Utah. In addition, the on-line performance of a commercial electrochemical monitor, the BIoGEORGE?, has been evaluated during extended deployments at geothermal facilities. This report provides a review of these techniques, presents data on their application from laboratory and field studies, and discusses their value in characterizing and monitoring biological activities at geothermal power plants.

  7. 2011 Archaea: Ecology, Metabolism, & Molecular Biology

    SciTech Connect

    Keneth Stedman

    2011-08-05

    Archaea, one of three major evolutionary lineages of life, are a fascinating and diverse group of microbes with deep roots overlapping those of eukaryotes. The focus of the 'Archaea: Ecology Metabolism & Molecular Biology' GRC conference expands on a number of emerging topics highlighting new paradigms in archaeal metabolism, genome function and systems biology; information processing; evolution and the tree of life; the ecology and diversity of archaea and their viruses. The strength of this conference lies in its ability to couple a field with a rich history in high quality research with new scientific findings in an atmosphere of stimulating exchange. This conference remains an excellent opportunity for younger scientists to interact with world experts in this field.

  8. 2009 Archaea: Ecology, Metabolism & Molecular Biology GRC

    SciTech Connect

    Dr. Julie Maupin- Furlow

    2009-07-26

    Archaea, one of three major evolutionary lineages of life, are a fascinating and diverse group of microbes with deep roots overlapping those of eukaryotes. The focus of the 'Archaea: Ecology Metabolism & Molecular Biology' GRC conference expands on a number of emerging topics highlighting new paradigms in archaeal metabolism, genome function and systems biology; information processing; evolution and the tree of life; the ecology and diversity of archaea and their viruses; and industrial applications. The strength of this conference lies in its ability to couple a field with a rich history in high quality research with new scientific findings in an atmosphere of stimulating exchange. This conference remains an excellent opportunity for younger scientists to interact with world experts in this field.

  9. Developing Biological Specifications for Fish Friendly Turbines

    SciTech Connect

    Neitzel, Duane A.

    2009-09-14

    This factsheet explains studies conducted in a highly reproducible manner to examine the biological effects to fish exposed to a shear environment in the laboratory. Strain rate was used as the index of intensity to describe the hydraulic force experienced by a fish in a shear environment. It was determined that no significant injuries occurred to any fish subjected to strain rates equal to or less than 500 cm/s/cm.

  10. Computational Tools to Assess Turbine Biological Performance

    SciTech Connect

    Richmond, Marshall C.; Serkowski, John A.; Rakowski, Cynthia L.; Strickler, Brad; Weisbeck, Molly; Dotson, Curtis L.

    2014-07-24

    Public Utility District No. 2 of Grant County (GCPUD) operates the Priest Rapids Dam (PRD), a hydroelectric facility on the Columbia River in Washington State. The dam contains 10 Kaplan-type turbine units that are now more than 50 years old. Plans are underway to refit these aging turbines with new runners. The Columbia River at PRD is a migratory pathway for several species of juvenile and adult salmonids, so passage of fish through the dam is a major consideration when upgrading the turbines. In this paper, a method for turbine biological performance assessment (BioPA) is demonstrated. Using this method, a suite of biological performance indicators is computed based on simulated data from a CFD model of a proposed turbine design. Each performance indicator is a measure of the probability of exposure to a certain dose of an injury mechanism. Using known relationships between the dose of an injury mechanism and frequency of injury (dose–response) from laboratory or field studies, the likelihood of fish injury for a turbine design can be computed from the performance indicator. By comparing the values of the indicators from proposed designs, the engineer can identify the more-promising alternatives. We present an application of the BioPA method for baseline risk assessment calculations for the existing Kaplan turbines at PRD that will be used as the minimum biological performance that a proposed new design must achieve.

  11. KPFM and PFM of Biological Systems

    SciTech Connect

    Rodriguez, Brian [University College, Dublin; Kalinin, Sergei V [ORNL

    2011-01-01

    Surface potentials and electrostatic interactions in biological systems are a key element of cellular regulation and interaction. Examples include cardiac and muscular activity, voltage-gated ion channels, protein folding and assembly, and electroactive cells and electrotransduction. The coupling between electrical, mechanical, and chemical signals and responses in cellular systems necessitates the development of tools capable of measuring the distribution of charged species, surface potentials, and mechanical responses to applied electrical stimuli and vice versa, ultimately under physiological conditions. In this chapter, applications of voltage-modulated atomic force microscopy (AFM) methods including Kelvin probe force microscopy (KPFM) and piezoresponse force microscopy (PFM) to biological systems are discussed. KPFM is a force-sensitive non-contact or intermittent-contact mode AFM technique that allows electrostatic interactions and surface potentials to be addressed. Beyond long-range electrostatic interactions, the application of bias can lead to a mechanical response, e.g., due to linear piezoelectric coupling in polar biopolymers or via more complex electrotransduction and redox pathways in other biosystems. The use and development of PFM, based on direct electromechanical detection, to biological systems will also be addressed. The similarities and limitations of measuring surface potentials and electromechanical coupling in solution will be outlined.

  12. Chemical and biological sensing using tuning forks

    DOEpatents

    Tao, Nongjian; Boussaad, Salah

    2012-07-10

    A device for sensing a chemical analyte is disclosed. The device is comprised of a vibrating structure having first and second surfaces and having an associated resonant frequency and a wire coupled between the first and second surfaces of the vibrating structure, wherein the analyte interacts with the wire and causes a change in the resonant frequency of the vibrating structure. The vibrating structure can include a tuning fork. The vibrating structure can be comprised of quartz. The wire can be comprised of polymer. A plurality of vibrating structures are arranged in an array to increase confidence by promoting a redundancy of measurement or to detect a plurality of chemical analytes. A method of making a device for sensing a chemical analyte is also disclosed.

  13. Nanocrystal structures

    DOEpatents

    Eisler, Hans J.; Sundar, Vikram C.; Walsh, Michael E.; Klimov, Victor I.; Bawendi, Moungi G.; Smith, Henry I.

    2008-12-30

    A structure including a grating and a semiconductor nanocrystal layer on the grating, can be a laser. The semiconductor nanocrystal layer can include a plurality of semiconductor nanocrystals including a Group II-VI compound, the nanocrystals being distributed in a metal oxide matrix. The grating can have a periodicity from 200 nm to 500 nm.

  14. Nanocrystal structures

    DOEpatents

    Eisler, Hans J.; Sundar, Vikram C.; Walsh, Michael E.; Klimov, Victor I.; Bawendi, Moungi G.; Smith, Henry I.

    2006-12-19

    A structure including a grating and a semiconductor nanocrystal layer on the grating, can be a laser. The semiconductor nanocrystal layer can include a plurality of semiconductor nanocrystals including a Group II–VI compound, the nanocrystals being distributed in a metal oxide matrix. The grating can have a periodicity from 200 nm to 500 nm.

  15. Nanocrystal structures

    DOEpatents

    Eisler, Hans J.; Sundar, Vikram C.; Walsh, Michael E.; Klimov, Victor I.; Bawendi, Moungi G.; Smith, Henry I.

    2006-12-19

    A structure including a grating and a semiconductor nanocrystal layer on the grating, can be a laser. The semiconductor nanocrystal layer can include a plurality of semiconductor nanocrystals including a Group IIVI compound, the nanocrystals being distributed in a metal oxide matrix. The grating can have a periodicity from 200 nm to 500 nm.

  16. Biological Imaging by Soft X-Ray Diffraction Microscopy

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Biological Imaging by Soft X-Ray Diffraction Microscopy Print Wednesday, 30 November 2005 00:00 Electron and x-ray...

  17. Sharpening Our Tools: Algal Biology Toolbox Workshop | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Sharpening Our Tools: Algal Biology Toolbox Workshop Sharpening Our Tools: Algal Biology Toolbox Workshop May 24, 2016 8:00AM PDT to May 25, 2016 4:00PM PDT Hard Rock Hotel San ...

  18. Algal Biology Program at Los Alamos gets a star

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Algal Biology Program gets a star Algal Biology Program at Los Alamos gets a star Richard Sayre, one of the nation's top specialists in algae and energy-producing plant research, ...

  19. Ringleader: Jay Nix, Beamline Director for the Molecular Biology...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Jay Nix, Beamline Director for the Molecular Biology Consortium Print Jay Nix started started the user program at Beamline 4.2.2 back in 2004, shortly after the Molecular Biology ...

  20. BioLogical Capital BLC | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    BioLogical Capital BLC Jump to: navigation, search Name: BioLogical Capital (BLC) Place: Denver, Colorado Zip: 80202 Product: Denver Colorado-based group focusing on generating...

  1. Generation and characterization of biological aerosols for laser measurements

    SciTech Connect

    Cheng, Yung-Sung; Barr, E.B.

    1995-12-01

    Concerns for proliferation of biological weapons including bacteria, fungi, and viruses have prompted research and development on methods for the rapid detection of biological aerosols in the field. Real-time instruments that can distinguish biological aerosols from background dust would be especially useful. Sandia National Laboratories (SNL) is developing a laser-based, real-time instrument for rapid detection of biological aerosols, and ITRI is working with SNL scientists and engineers to evaluate this technology for a wide range of biological aerosols. This paper describes methods being used to generate the characterize the biological aerosols for these tests. In summary, a biosafe system has been developed for generating and characterizing biological aerosols and using those aerosols to test the SNL laser-based real-time instrument. Such tests are essential in studying methods for rapid detection of airborne biological materials.

  2. Ringleader: Jay Nix, Beamline Director for the Molecular Biology...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    the Molecular Biology Consortium Print Jay Nix started started the user program at Beamline 4.2.2 back in 2004, shortly after the Molecular Biology Consortium built the beamline. ...

  3. Biological Conversion of Sugars to Hydrocarbons Technology Pathway

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    ... to leverage experience in biochemical processing, specifically cellulose and ... in downstream biological conversion and improving overall process integration. ...

  4. 2013 Biological Hydrogen Production Workshop Summary Report | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Energy Biological Hydrogen Production Workshop Summary Report 2013 Biological Hydrogen Production Workshop Summary Report November 2013 summary report for the 2013 Biological Hydrogen Production Workshop. bio_h2_workshop_final_report.pdf (1.55 MB) More Documents & Publications The Hydrogen Program at NREL: A Brief Overview Hydrogenases and Barriers for Biotechnological Hydrogen Production Technologies Renewable Hydrogen Production from Biological Systems

  5. Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 b2blowres63006.pdf (8.11 MB) More Documents & Publications Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Cellulosic Sugar and Lignin Production Capabilities RFI Responses Review of Recent Pilot Scale Cellulosic Ethanol Demonstration

  6. National Association of Biology Teachers Conference | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Association of Biology Teachers Conference National Association of Biology Teachers Conference November 3, 2016 8:00AM MST to November 6, 2016 12:00PM MST Denver Sheraton-Downtown 1550 Court Pl Denver, Colorado 80202 The National Association of Biology Teachers Conference will be hosted in Denver, Colorado, November 3-6, 2016. The conference will provide an opportunity for leaders in biology and life science education to participate in hands-on workshops and informational sessions. Bioenergy

  7. Hydro Review: Computational Tools to Assess Turbine Biological Performance

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    | Department of Energy Hydro Review: Computational Tools to Assess Turbine Biological Performance Hydro Review: Computational Tools to Assess Turbine Biological Performance This review covers the BioPA method used to analyze the biological performance of proposed designs to help ensure the safety of fish passing through the turbines at the Priest Rapids Dam in Grant County, Washington. Computational Tools to Assess Turbine Biological Performance (483.71 KB) More Documents & Publications

  8. Superconducting structure

    DOEpatents

    Kwon, Chuhee; Jia, Quanxi; Foltyn, Stephen R.

    2003-04-01

    A superconductive structure including a dielectric oxide substrate, a thin buffer layer of a superconducting material thereon; and, a layer of a rare earth-barium-copper oxide superconducting film thereon the thin layer of yttrium-barium-copper oxide, the rare earth selected from the group consisting of samarium, gadolinium, ytterbium, erbium, neodymium, dysprosium, holmium, lutetium, a combination of more than one element from the rare earth group and a combination of one or more elements from the rare earth group with yttrium, the buffer layer of superconducting material characterized as having chemical and structural compatibility with the dielectric oxide substrate and the rare earth-barium-copper oxide superconducting film is provided.

  9. Superconducting Structure

    DOEpatents

    Kwon, Chuhee; Jia, Quanxi; Foltyn, Stephen R.

    2005-09-13

    A superconductive structure including a dielectric oxide substrate, a thin buffer layer of a superconducting material thereon; and, a layer of a rare earth-barium-copper oxide superconducting film thereon the thin layer of yttrium-barium-copper oxide, the rare earth selected from the group consisting of samarium, gadolinium, ytterbium, erbium, neodymium, dysprosium, holmium, lutetium, a combination of more than one element from the rare earth group and a combination of one or more elements from the rare earth group with yttrium, the buffer layer of superconducting material characterized as having chemical and structural compatibility with the dielectric oxide substrate and the rare earth-barium-copper oxide superconducting film is provided.

  10. Terminal structure

    DOEpatents

    Schmidt, Frank; Allais, Arnaud; Mirebeau, Pierre; Ganhungu, Francois; Lallouet, Nicolas

    2009-10-20

    A terminal structure (2) for a superconducting cable (1) is described. It consists of a conductor (2a) and an insulator (2b) that surrounds the conductor (2a), wherein the superconducting cable (1) has a core with a superconducting conductor (5) and a layer of insulation that surrounds the conductor (5), and wherein the core is arranged in such a way that it can move longitudinally in a cryostat. The conductor (2a) of the terminal structure (2) is electrically connected with the superconducting conductor (5) or with a normal conductor (6) that is connected with the superconducting conductor (5) by means of a tubular part (7) made of an electrically conductive material, wherein the superconducting conductor (5) or the normal conductor (6) can slide in the part (7) in the direction of the superconductor.

  11. AN INTEGRATED BIOLOGICAL CONTROL SYSTEM AT HANFORD

    SciTech Connect

    JOHNSON AR; CAUDILL JG; GIDDINGS RF; RODRIGUEZ JM; ROOS RC; WILDE JW

    2010-02-11

    In 1999 an integrated biological control system was instituted at the U.S. Department of Energy's Hanford Site. Successes and changes to the program needed to be communicated to a large and diverse mix of organizations and individuals. Efforts at communication are directed toward the following: Hanford Contractors (Liquid or Tank Waste, Solid Waste, Environmental Restoration, Science and Technology, Site Infrastructure), General Hanford Employees, and Hanford Advisory Board (Native American Tribes, Environmental Groups, Local Citizens, Washington State and Oregon State regulatory agencies). Communication was done through direct interface meetings, individual communication, where appropriate, and broadly sharing program reports. The objectives of the communication efforts was to have the program well coordinated with Hanford contractors, and to have the program understood well enough that all stakeholders would have confidence in the work performed by the program to reduce or elimated spread of radioactive contamination by biotic vectors. Communication of successes and changes to an integrated biological control system instituted in 1999 at the Department of Energy's Hanford Site have required regular interfaces with not only a diverse group of Hanford contractors (i.e., those responsible for liquid or tank waste, solid wastes, environmental restoration, science and technology, and site infrastructure), and general Hanford employees, but also with a consortium of designated stake holders organized as the Hanford Advisory Board (i.e., Native American tribes, various environmental groups, local citizens, Washington state and Oregon regulatory agencies, etc.). Direct interface meetings, individual communication where appropriate, and transparency of the biological control program were the methods and outcome of this effort.

  12. Historical Information H.2 Biological Studies

    Office of Legacy Management (LM)

    _-_ . - H.2 Biological Studies (0 \ j ; : : j Book . l Ad Hoc Rulison Review Panel Comments Regarding Re-Entry , and Testing Operations, December 22, 1969 This page intentionally left blank December 22, 1969 M r . Robert E. Miller, Manager Nevada Operations Office U. S. Atomic Energy Commission Post Office Box 14100 Las Vegas, Nevada 89114 Dear M r . Miller: F4cmbers of t h e A d Hoc Rulison Review Panel reconvened on December 22, 1969, t o hear and discuss comments r e s u l t i n g from our

  13. Synchronous Behavior of Two Coupled Biological Neurons

    SciTech Connect

    Elson, R.C.; Selverston, A.I.; Elson, R.C.; Selverston, A.I.; Huerta, R.; Rulkov, N.F.; Rabinovich, M.I.; Abarbanel, H.D.; Selverston, A.I.; Huerta, R.; Abarbanel, H.D.

    1998-12-01

    We report experimental studies of synchronization phenomena in a pair of biological neurons that interact through naturally occurring, electrical coupling. When these neurons generate irregular bursts of spikes, the natural coupling synchronizes slow oscillations of membrane potential, but not the fast spikes. By adding artificial electrical coupling we studied transitions between synchrony and asynchrony in both slow oscillations and fast spikes. We discuss the dynamics of bursting and synchronization in living neurons with distributed functional morphology. {copyright} {ital 1998} {ital The American Physical Society}

  14. LANSCE | Lujan Center | Biology Preparation Laboratory

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Biology Preparation Laboratory The Lujan Center Biolab offers a variety of capabilities. 1) Biodeuteration Lab (BDL) We run a protein expression lab for perdeuteration of user proteins. We offer full perdeuteration (~99%) using our algal-based media for bacterial growth. We also have M9 minimal media made in D2O for expression of up to ~85% perdeuteration. Users can use our lab in person or mail-in a plasmid for us to express for them. We also have standard protein expression equipment:

  15. Lateral organization, bilayer asymmetry, and inter-leaflet coupling of biological membranes

    DOE PAGES [OSTI]

    Smith, Jeremy C.; Cheng, Xiaolin; Nickels, Jonathan D.

    2015-07-29

    Understanding of cell membrane organization has evolved significantly from the classic fluid mosaic model. It is now recognized that biological membranes are highly organized structures, with differences in lipid compositions between inner and outer leaflets and in lateral structures within the bilayer plane, known as lipid rafts. These organizing principles are important for protein localization and function as well as cellular signaling. However, the mechanisms and biophysical basis of lipid raft formation, structure, dynamics and function are not clearly understood. One key question, which we focus on in this review, is how lateral organization and leaflet compositional asymmetry are coupled.more » Detailed information elucidating this question has been sparse because of the small size and transient nature of rafts and the experimental challenges in constructing asymmetric bilayers. Resolving this mystery will require advances in both experimentation and modeling. We discuss here the preparation of model systems along with experimental and computational approaches that have been applied in efforts to address this key question in membrane biology. Furthermore, we seek to place recent and future advances in experimental and computational techniques in context, providing insight into in-plane and transverse organization of biological membranes.« less

  16. Lateral organization, bilayer asymmetry, and inter-leaflet coupling of biological membranes

    SciTech Connect

    Smith, Jeremy C.; Cheng, Xiaolin; Nickels, Jonathan D.

    2015-07-29

    Understanding of cell membrane organization has evolved significantly from the classic fluid mosaic model. It is now recognized that biological membranes are highly organized structures, with differences in lipid compositions between inner and outer leaflets and in lateral structures within the bilayer plane, known as lipid rafts. These organizing principles are important for protein localization and function as well as cellular signaling. However, the mechanisms and biophysical basis of lipid raft formation, structure, dynamics and function are not clearly understood. One key question, which we focus on in this review, is how lateral organization and leaflet compositional asymmetry are coupled. Detailed information elucidating this question has been sparse because of the small size and transient nature of rafts and the experimental challenges in constructing asymmetric bilayers. Resolving this mystery will require advances in both experimentation and modeling. We discuss here the preparation of model systems along with experimental and computational approaches that have been applied in efforts to address this key question in membrane biology. Furthermore, we seek to place recent and future advances in experimental and computational techniques in context, providing insight into in-plane and transverse organization of biological membranes.

  17. Third international congress of plant molecular biology: Molecular biology of plant growth and development

    SciTech Connect

    Hallick, R.B.

    1995-02-01

    The Congress was held October 6-11, 1991 in Tucson with approximately 3000 scientists attending and over 300 oral presentations and 1800 posters. Plant molecular biology is one of the most rapidly developing areas of the biological sciences. Recent advances in the ability to isolate genes, to study their expression, and to create transgenic plants have had a major impact on our understanding of the many fundamental plant processes. In addition, new approaches have been created to improve plants for agricultural purposes. This is a book of presentation and posters from the conference.

  18. Acceptance Criteria Framework for Autonomous Biological Detectors

    SciTech Connect

    Dzenitis, J M

    2006-12-12

    The purpose of this study was to examine a set of user acceptance criteria for autonomous biological detection systems for application in high-traffic, public facilities. The test case for the acceptance criteria was the Autonomous Pathogen Detection System (APDS) operating in high-traffic facilities in New York City (NYC). However, the acceptance criteria were designed to be generally applicable to other biological detection systems in other locations. For such detection systems, ''users'' will include local authorities (e.g., facility operators, public health officials, and law enforcement personnel) and national authorities [including personnel from the Department of Homeland Security (DHS), the BioWatch Program, the Centers for Disease Control and Prevention (CDC), and the Federal Bureau of Investigation (FBI)]. The panel members brought expertise from a broad range of backgrounds to complete this picture. The goals of this document are: (1) To serve as informal guidance for users in considering the benefits and costs of these systems. (2) To serve as informal guidance for developers in understanding the needs of users. In follow-up work, this framework will be used to systematically document the APDS for appropriateness and readiness for use in NYC.

  19. Biological and Environmental Research Network Requirements

    SciTech Connect

    Balaji, V.; Boden, Tom; Cowley, Dave; Dart, Eli; Dattoria, Vince; Desai, Narayan; Egan, Rob; Foster, Ian; Goldstone, Robin; Gregurick, Susan; Houghton, John; Izaurralde, Cesar; Johnston, Bill; Joseph, Renu; Kleese-van Dam, Kerstin; Lipton, Mary; Monga, Inder; Pritchard, Matt; Rotman, Lauren; Strand, Gary; Stuart, Cory; Tatusova, Tatiana; Tierney, Brian; Thomas, Brian; Williams, Dean N.; Zurawski, Jason

    2013-09-01

    The Energy Sciences Network (ESnet) is the primary provider of network connectivity for the U.S. Department of Energy (DOE) Office of Science (SC), the single largest supporter of basic research in the physical sciences in the United States. In support of SC programs, ESnet regularly updates and refreshes its understanding of the networking requirements of the instruments, facilities, scientists, and science programs that it serves. This focus has helped ESnet be a highly successful enabler of scientific discovery for over 25 years. In November 2012, ESnet and the Office of Biological and Environmental Research (BER) of the DOE SC organized a review to characterize the networking requirements of the programs funded by the BER program office. Several key findings resulted from the review. Among them: 1) The scale of data sets available to science collaborations continues to increase exponentially. This has broad impact, both on the network and on the computational and storage systems connected to the network. 2) Many science collaborations require assistance to cope with the systems and network engineering challenges inherent in managing the rapid growth in data scale. 3) Several science domains operate distributed facilities that rely on high-performance networking for success. Key examples illustrated in this report include the Earth System Grid Federation (ESGF) and the Systems Biology Knowledgebase (KBase). This report expands on these points, and addresses others as well. The report contains a findings section as well as the text of the case studies discussed at the review.

  20. Airfoil structure

    DOEpatents

    Frey, Gary A.; Twardochleb, Christopher Z.

    1998-01-01

    Past airfoil configurations have been used to improve aerodynamic performance and engine efficiencies. The present airfoil configuration further increases component life and reduces maintenance by reducing internal stress within the airfoil itself. The airfoil includes a chord and a span. Each of the chord and the span has a bow being summed to form a generally "C" configuration of the airfoil. The generally "C" configuration includes a compound bow in which internal stresses resulting from a thermal temperature gradient are reduced. The structural configuration reduces internal stresses resulting from thermal expansion.

  1. Airfoil structure

    DOEpatents

    Frey, G.A.; Twardochleb, C.Z.

    1998-01-13

    Past airfoil configurations have been used to improve aerodynamic performance and engine efficiencies. The present airfoil configuration further increases component life and reduces maintenance by reducing internal stress within the airfoil itself. The airfoil includes a chord and a span. Each of the chord and the span has a bow being summed to form a generally ``C`` configuration of the airfoil. The generally ``C`` configuration includes a compound bow in which internal stresses resulting from a thermal temperature gradient are reduced. The structural configuration reduces internal stresses resulting from thermal expansion. 6 figs.

  2. STRUCTURE-SOIL-STRUCTURE INTERACTION AT SRS | Department of Energy

    Office of Environmental Management (EM)

    STRUCTURE-SOIL-STRUCTURE INTERACTION AT SRS STRUCTURE-SOIL-STRUCTURE INTERACTION AT SRS Structure-Soil-Structure Interaction at SRS Structural Mechanics - SRS October 25, 2011 ...

  3. ANAEROBIC BIOLOGICAL TREATMENT OF PRODUCED WATER

    SciTech Connect

    John R. Gallagher

    2001-07-31

    During the production of oil and gas, large amounts of water are brought to the surface and must be disposed of in an environmentally sensitive manner. This is an especially difficult problem in offshore production facilities where space is a major constraint. The chief regulatory criterion for produced water is oil and grease. Most facilities have little trouble meeting this criterion using conventional oil-water separation technologies. However, some operations have significant amounts of naphthenic acids in the water that behave as oil and grease but are not well removed by conventional technologies. Aerobic biological treatment of naphthenic acids in simulated-produced water has been demonstrated by others; however, the system was easily overloaded by the large amounts of low-molecular-weight organic acids often found in produced waters. The objective of this research was to determine the ability of an anaerobic biological system to treat these organic acids in a simulated produced water and to examine the potential for biodegradation of the naphthenic acids in the anaerobic environment. A small fixed-film anaerobic biological reactor was constructed and adapted to treat a simulated produced water. The bioreactor was tubular, with a low-density porous glass packing material. The inocula to the reactor was sediment from a produced-water holding pond from a municipal anaerobic digester and two salt-loving methanogenic bacteria. During start-up, the feed to the reactor contained glucose as well as typical produced-water components. When glucose was used, rapid gas production was observed. However, when glucose was eliminated and the major organic component was acetate, little gas was generated. Methane production from acetate may have been inhibited by the high salt concentrations, by sulfide, or because of the lack, despite seeding, of microbes capable of converting acetate to methane. Toluene, a minor component of the produced water (0.1 g/L) was removed in the

  4. Documentation of TRU biological transport model (BIOTRAN)

    SciTech Connect

    Gallegos, A.F.; Garcia, B.J.; Sutton, C.M.

    1980-01-01

    Inclusive of Appendices, this document describes the purpose, rationale, construction, and operation of a biological transport model (BIOTRAN). This model is used to predict the flow of transuranic elements (TRU) through specified plant and animal environments using biomass as a vector. The appendices are: (A) Flows of moisture, biomass, and TRU; (B) Intermediate variables affecting flows; (C) Mnemonic equivalents (code) for variables; (D) Variable library (code); (E) BIOTRAN code (Fortran); (F) Plants simulated; (G) BIOTRAN code documentation; (H) Operating instructions for BIOTRAN code. The main text is presented with a specific format which uses a minimum of space, yet is adequate for tracking most relationships from their first appearance to their formulation in the code. Because relationships are treated individually in this manner, and rely heavily on Appendix material for understanding, it is advised that the reader familiarize himself with these materials before proceeding with the main text.

  5. Quantifying evolvability in small biological networks

    SciTech Connect

    Nemenman, Ilya; Mugler, Andrew; Ziv, Etay; Wiggins, Chris H

    2008-01-01

    The authors introduce a quantitative measure of the capacity of a small biological network to evolve. The measure is applied to a stochastic description of the experimental setup of Guet et al. (Science 2002, 296, pp. 1466), treating chemical inducers as functional inputs to biochemical networks and the expression of a reporter gene as the functional output. The authors take an information-theoretic approach, allowing the system to set parameters that optimise signal processing ability, thus enumerating each network's highest-fidelity functions. All networks studied are highly evolvable by the measure, meaning that change in function has little dependence on change in parameters. Moreover, each network's functions are connected by paths in the parameter space along which information is not significantly lowered, meaning a network may continuously change its functionality without completely losing it along the way. This property further underscores the evolvability of the networks.

  6. Overview of selected molecular biological databases

    SciTech Connect

    Rayl, K.D.; Gaasterland, T.

    1994-11-01

    This paper presents an overview of the purpose, content, and design of a subset of the currently available biological databases, with an emphasis on protein databases. Databases included in this summary are 3D-ALI, Berlin RNA databank, Blocks, DSSP, EMBL Nucleotide Database, EMP, ENZYME, FSSP, GDB, GenBank, HSSP, LiMB, PDB, PIR, PKCDD, ProSite, and SWISS-PROT. The goal is to provide a starting point for researchers who wish to take advantage of the myriad available databases. Rather than providing a complete explanation of each database, we present its content and form by explaining the details of typical entries. Pointers to more complete ``user guides`` are included, along with general information on where to search for a new database.

  7. Recovery from chemical, biological, and radiological incidents :

    SciTech Connect

    Franco, David Oliver; Yang, Lynn I.; Hammer, Ann E.

    2012-06-01

    To restore regional lifeline services and economic activity as quickly as possible after a chemical, biological or radiological incident, emergency planners and managers will need to prioritize critical infrastructure across many sectors for restoration. In parallel, state and local governments will need to identify and implement measures to promote reoccupation and economy recovery in the region. This document provides guidance on predisaster planning for two of the National Disaster Recovery Framework Recovery Support Functions: Infrastructure Systems and Economic Recovery. It identifies key considerations for infrastructure restoration, outlines a process for prioritizing critical infrastructure for restoration, and identifies critical considerations for promoting regional economic recovery following a widearea disaster. Its goal is to equip members of the emergency preparedness community to systematically prioritize critical infrastructure for restoration, and to develop effective economic recovery plans in preparation for a widearea CBR disaster.

  8. Biological Systems for Hydrogen Photoproduction (Presentation)

    SciTech Connect

    Ghirardi, M. L.

    2012-05-01

    This presentation summarizes NREL biological systems for hydrogen photoproduction work for the DOE Hydrogen and Fuel Cells Program Annual Merit Review and Peer Evaluation Meeting, May 14-18, 2012. General goal is develop photobiological systems for large-scale, low cost and efficient H{sub 2} production from water (barriers AH, AI and AJ). Specific tasks are: (1) Address the O{sub 2} sensitivity of hydrogenases that prevent continuity of H{sub 2} photoproduction under aerobic, high solar-to-hydrogen (STH) light conversion efficiency conditions; and (2) Utilize a limited STH H{sub 2}-producing method (sulfur deprivation) as a platform to address or test other factors limiting commercial algal H{sub 2} photoproduction, including low rates due to biochemical and engineering mechanisms.

  9. Biological effects of electric fields: EPRI's role

    SciTech Connect

    Kavet, R.

    1982-07-01

    Since 1973 the Electric Power Research Institute (EPRI) has supported research to evaluate the biological effects which may result from exposure to electric fields produced by AC overhead transmission lines; more recently, EPRI has also begun DC research. Through 1981 EPRI will have expended $8.7M on these efforts. Ongoing AC projects are studying a variety of lifeforms exposed to electric fields; these include humans, miniature swine, rats, honeybees, chick embryos, and crops. The status of these projects is discussed. The DC program has not as yet produced data. These studies will add to the current data base so as to enable a more complete assessment of health risks which may be associated with exposure to electric fields at power frequencies.

  10. Biology and Medicine Division annual report, 1985

    SciTech Connect

    Not Available

    1986-04-01

    This book briefly describes the activities of the Biology and Medicine Division of the Lawrence Berkeley Laboratory. During the past year the Donner Pavilion program on the treatment of arteriovenous malformations in the brain has chalked up very significant successes. The disease control rate has been high and objective measures of success using cerebral angiography have been established. The new high resolution positron emitting tomographic imager has been demonstrated to operate successfully. In the Radiation Biophysics program, the availability of higher mass ions up to uranium has allowed us cell and tissue studies in a radiation domain that is entirely new. Using uranium beams, investigators have already made new and exciting findings that are described in the body of the report.

  11. Armor structures

    DOEpatents

    Chu, Henry Shiu-Hung [Idaho Falls, ID; Lacy, Jeffrey M [Idaho Falls, ID

    2008-04-01

    An armor structure includes first and second layers individually containing a plurality of i-beams. Individual i-beams have a pair of longitudinal flanges interconnected by a longitudinal crosspiece and defining opposing longitudinal channels between the pair of flanges. The i-beams within individual of the first and second layers run parallel. The laterally outermost faces of the flanges of adjacent i-beams face one another. One of the longitudinal channels in each of the first and second layers faces one of the longitudinal channels in the other of the first and second layers. The channels of the first layer run parallel with the channels of the second layer. The flanges of the first and second layers overlap with the crosspieces of the other of the first and second layers, and portions of said flanges are received within the facing channels of the i-beams of the other of the first and second layers.

  12. Plant biology research and training for the 21st century

    SciTech Connect

    Kelly, K.

    1992-01-01

    The committee was assembled in response to a request from the National Science Foundation (NSF), the US Department of Agriculture (USDA), and the US Department of Energy (DoE). The leadership of these agencies asked the National Academy of Sciences through the National Research Council (NRC) to assess the status of plant-science research in the United States in light of the opportunities arising from advances inother areas of biology. NRC was asked to suggest ways of accelerating the application of these new biologic concepts and tools to research in plant science with the aim of enhancing the acquisition of new knowledge about plants. The charge to the committee was to examine the following: Organizations, departments, and institutions conducting plant biology research; human resources involved in plant biology research; graduate training programs in plant biology; federal, state, and private sources of support for plant-biology research; the role of industry in conducting and supporting plant-biology research; the international status of US plant-biology research; and the relationship of plant biology to leading-edge research in biology.

  13. Plant biology research and training for the 21st century

    SciTech Connect

    Kelly, K.

    1992-12-31

    The committee was assembled in response to a request from the National Science Foundation (NSF), the US Department of Agriculture (USDA), and the US Department of Energy (DoE). The leadership of these agencies asked the National Academy of Sciences through the National Research Council (NRC) to assess the status of plant-science research in the United States in light of the opportunities arising from advances inother areas of biology. NRC was asked to suggest ways of accelerating the application of these new biologic concepts and tools to research in plant science with the aim of enhancing the acquisition of new knowledge about plants. The charge to the committee was to examine the following: Organizations, departments, and institutions conducting plant biology research; human resources involved in plant biology research; graduate training programs in plant biology; federal, state, and private sources of support for plant-biology research; the role of industry in conducting and supporting plant-biology research; the international status of US plant-biology research; and the relationship of plant biology to leading-edge research in biology.

  14. Solving the RNA polymerase I structural puzzle

    SciTech Connect

    Moreno-Morcillo, Mara; Taylor, Nicholas M. I.; Gruene, Tim; Legrand, Pierre; Rashid, Umar J.; Ruiz, Federico M.; Steuerwald, Ulrich; Mller, Christoph W.; Fernndez-Tornero, Carlos

    2014-10-01

    Details of the RNA polymerase I crystal structure determination provide a framework for solution of the structures of other multi-subunit complexes. Simple crystallographic experiments are described to extract relevant biological information such as the location of the enzyme active site. Knowing the structure of multi-subunit complexes is critical to understand basic cellular functions. However, when crystals of these complexes can be obtained they rarely diffract beyond 3 resolution, which complicates X-ray structure determination and refinement. The crystal structure of RNA polymerase I, an essential cellular machine that synthesizes the precursor of ribosomal RNA in the nucleolus of eukaryotic cells, has recently been solved. Here, the crucial steps that were undertaken to build the atomic model of this multi-subunit enzyme are reported, emphasizing how simple crystallographic experiments can be used to extract relevant biological information. In particular, this report discusses the combination of poor molecular replacement and experimental phases, the application of multi-crystal averaging and the use of anomalous scatterers as sequence markers to guide tracing and to locate the active site. The methods outlined here will likely serve as a reference for future structural determination of large complexes at low resolution.

  15. Methods of biological dosimetry employing chromosome-specific staining

    DOEpatents

    Gray, Joe W.; Pinkel, Daniel

    2000-01-01

    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods are provided to disable the hybridization capacity of shared, high copy repetitive sequences and/or remove such sequences to provide for useful contrast. Still further methods are provided to produce chromosome-specific staining reagents which are made specific to the targeted chromosomal material, which can be one or more whole chromosomes, one or more regions on one or more chromosomes, subsets of chromosomes and/or the entire genome. Probes and test kits are provided for use in tumor cytogenetics, in the detection of disease related loci, in analysis of structural abnormalities, such as translocations, and for biological dosimetry. Further, methods and prenatal test kits are provided to stain targeted chromosomal material of fetal cells, including fetal cells obtained from maternal blood. Still further, the invention provides for automated means to detect and analyse chromosomal abnormalities.

  16. Nonlinear, noniterative, single-distance phase retrieval and developmental biology

    SciTech Connect

    Moosmann, Julian; Altapova, Venera; Haenschke, Daniel; Hofmann, Ralf; Baumbach, Tilo

    2012-05-17

    For coherent X-ray imaging, based on phase contrast through free-space Fresnel propagation, we discuss two noniterative, nonlinear approaches to the phase-retrieval problem from a single-distance intensity map of a pure-phase object. On one hand, a perturbative set-up is proposed where nonlinear corrections to the linearized transport-of-intensity situation are expanded in powers of the object-detector distance z and are evaluated in terms of the linear estimate. On the other hand, a nonperturbative projection algorithm, which is based on the (linear and local) contrast-transfer function (CTF), works with an effective phase in Fourier space. This effective phase obeys a modified CTF relation between intensity contrast at z > 0 and phase contrast at z= 0: Unphysical singularities of the local CTF model are cut off to yield 'quasiparticles' in analogy to the theory of the Fermi liquid. By identifying the positions of the zeros of the Fourier transformed intensity contrast as order parameters for the dynamical breaking of scaling symmetry we investigate the phase structure of the forward-propagation problem when interpreted as a statistical system. Results justify the quasiparticle approach for a wide range of intermediary phase variations. The latter algorithm is applied to data from biological samples recorded at the beamlines TopoTomo and ID19 at ANKA and ESRF, respectively.

  17. Bioforensics: Characterization of biological weapons agents by NanoSIMS

    SciTech Connect

    Weber, P K; Ghosal, S; Leighton, T J; Wheeler, K E; Hutcheon, I D

    2007-02-26

    The anthrax attacks of Fall 2001 highlight the need to develop forensic methods based on multiple identifiers to determine the origin of biological weapons agents. Genetic typing methods (i.e., DNA and RNA-based) provide one attribution technology, but genetic information alone is not usually sufficient to determine the provenance of the material. Non-genetic identifiers, including elemental and isotopic signatures, provide complementary information that can be used to identify the means, geographic location and date of production. Under LDRD funding, we have successfully developed the techniques necessary to perform bioforensic characterization with the NanoSIMS at the individual spore level. We have developed methods for elemental and isotopic characterization at the single spore scale. We have developed methods for analyzing spore sections to map elemental abundance within spores. We have developed rapid focused ion beam (FIB) sectioning techniques for spores to preserve elemental and structural integrity. And we have developed a high-resolution depth profiling method to characterize the elemental distribution in individual spores without sectioning. We used these newly developed methods to study the controls on elemental abundances in spores, characterize the elemental distribution of in spores, and to study elemental uptake by spores. Our work under this LDRD project attracted FBI and DHS funding for applied purposes.

  18. Crystal Structure of a Conserved Phosphatase Domain of Non-structural

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Protein-3 (nsp3) from the SARS Coronavirus a Conserved Phosphatase Domain of Non-structural Protein-3 (nsp3) from the SARS Coronavirus Kumar Singh Saikatendu1, Jeremiah S. Joseph1, Vanitha Subramanian1, Benjamin W. Neuman3, Michael J. Buchmeier3, Raymond C. Stevens2 and Peter Kuhn1. 1Departments of Cell Biology, 2Molecular Biology, and 3Neuropharmacology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037 Severe Acute Respiratory Syndrome (SARS) is a

  19. OSTIblog Articles in the Office of Biological and Environmental Research

    Office of Scientific and Technical Information (OSTI)

    (OBER) Abstracts Database Topic | OSTI, US Dept of Energy Office of Scientific and Technical Information Biological and Environmental Research (OBER) Abstracts Database Topic Managing the Department of Energy's Office of Biological and Environmental Research (BER) Project Information by Lorrie Johnson 02 Aug, 2011 in Science Communications 4339 BER%20banner.jpg Managing the Department of Energy's Office of Biological and Environmental Research (BER) Project Information Read more about 4339

  20. Biological and Environmental Research Advisory Committee (BERAC) meeting

    Office of Science (SC)

    October 27-28, 2016| U.S. DOE Office of Science (SC) Biological and Environmental Research Advisory Committee (BERAC) meeting October 27-28, 2016 Rockville, Md. Biological and Environmental Research Advisory Committee (BERAC) BERAC Home Meetings BERAC Minutes BERAC Minutes Archive Members Charges/Reports Charter .pdf file (135KB) BER Committees of Visitors Federal Advisory Committees BER Home Meetings Biological and Environmental Research Advisory Committee (BERAC) meeting October 27-28,

  1. California Valley Solar Ranch Biological Assessment | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    California Valley Solar Ranch Biological Assessment California Valley Solar Ranch Biological Assessment Biological Assessment for the California Valley Solar Ranch Project San Luis Obispo County, California High Plains Ranch II, LLC (HPR II), a wholly owned subsidiary of SunPower Corporation, Systems ("SunPower") proposes to construct a 250-megawatt (MW) solar photovoltaic (PV) energy plant, the California Valley Solar Ranch Project (CVSR Project or Project), on a 4,747acre site in

  2. Damage-free vibrational spectroscopy of biological materials in the

    Office of Scientific and Technical Information (OSTI)

    electron microscope (Journal Article) | SciTech Connect Damage-free vibrational spectroscopy of biological materials in the electron microscope Citation Details In-Document Search Title: Damage-free vibrational spectroscopy of biological materials in the electron microscope Vibrational spectroscopy in the electron microscope would be transformative in the study of biological samples, provided that radiation damage could be prevented. However, electron beams typically create high-energy

  3. Synthetic biology and crop engineering | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Synthetic biology and crop engineering Synthetic biology and crop engineering Breakout Session 2: Frontiers and Horizons Session 2-A: Synthetic Biology and the Promise of Biofuels Jonathan Burbaum, Program Director, Department of Energy, Office of Science, ARPA-E b13_burbaum_2-a.pdf (1.63 MB) More Documents & Publications EIS-0481: Final Programmatic Environmental Impact Statement EIS-0481: Draft Programmatic Environmental Impact Statement EIS-0481: Notice of Intent to Prepare a Programmatic

  4. WILD PIGS: BIOLOGY, DAMAGE, CONTROL TECHINQUES AND MANAGEMENT

    SciTech Connect

    Mayer, John; Brisbin, I. Lehr

    2009-12-31

    about anything; and, they can live just about anywhere. On top of that, wild pigs are both very difficult to control and, with the possible exception of island ecosystems, almost impossible to eradicate (Dickson et al. 2001, Sweeney et al. 2003). The solution to the wild pig problem has not been readily apparent. The ultimate answer as to how to control these animals has not been found to date. In many ways, wild pigs are America's most successful large invasive species. All of which means that wild pigs are a veritable nightmare for land and resource managers trying to keep the numbers of these animals and the damage that they do under control. Since the more that one knows about an invasive species, the easier it is to deal with and hopefully control. For wild pigs then, it is better to 'know thy enemy' than to not, especially if one expects to be able to successfully control them. In an effort to better 'know thy enemy,' a two-day symposium was held in Augusta, Georgia, on April 21-22, 2004. This symposium was organized and sponsored by U.S.D.A. Forest Service-Savannah River (USFS-SR), U. S. Department of Energy-Savannah River Operations Office (DOE-SR), the Westinghouse Savannah River Company (WSRC), the South Carolina Chapter of the Soil & Water Conservation Society, and the Savannah River Ecology Laboratory (SREL). The goal of this symposium was to assemble researchers and land managers to first address various aspects of the biology and damage of wild pigs, and then review the control techniques and management of this invasive species. The result would then be a collected synopsis of what is known about wild pigs in the United States. Although the focus of the symposium was primarily directed toward federal agencies, presenters also included professionals from academic institutions, and private-sector control contractors and land managers. Most of the organizations associated with implementing this symposium were affiliated with the Savannah River Site (SRS), a

  5. Synthesis, crystal structure, resistivity, and electronic structure...

    Office of Scientific and Technical Information (OSTI)

    Synthesis, crystal structure, resistivity, and electronic structure of the U(V) quaternary ... and electronic structure of the U(V) quaternary polyselenide Ba8PdU2Se12(Se2)2 ...

  6. Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen Production

    SciTech Connect

    James, B. D.; Baum, G. N.; Perez, J.; Baum, K. N.

    2009-09-01

    Report documenting the biological and engineering characteristics of five algal and bacterial hydrogen production systems selected by DOE and NREL for evaluation.

  7. Most Viewed Documents for Biology and Medicine: September 2014...

    Office of Scientific and Technical Information (OSTI)

    for Biology and Medicine: September 2014 Modification to the Monte Carlo N-Particle (MCNP) Visual Editor (MCNPVised) to Read in Computer Aided Design (CAD) Files Randolph Schwarz; ...

  8. Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen Production

    Energy.gov [DOE]

    Report documenting the biological and engineering characteristics of five algal and bacterial hydrogen production systems selected by DOE and NREL for evaluation.

  9. March 2014 Most Viewed Documents for Biology And Medicine | OSTI...

    Office of Scientific and Technical Information (OSTI)

    March 2014 Most Viewed Documents for Biology And Medicine Carbon Dioxide Sequestering Using Microalgal Systems Daniel J. Stepan; Richard E. Shockey; Thomas A. Moe; Ryan Dorn (2002) ...

  10. September 2015 Most Viewed Documents for Biology And Medicine...

    Office of Scientific and Technical Information (OSTI)

    September 2015 Most Viewed Documents for Biology And Medicine Measuring dopamine release in the human brain with PET Volkow, N.D. Brookhaven National Lab., Upton, NY (United ...

  11. DOE Issues Request for Information on Biological Hydrogen Production

    Energy.gov [DOE]

    The Fuel Cell Technologies Office has issued a request for information seeking feedback from interested stakeholders regarding biological hydrogen production research and development.

  12. April 2013 Most Viewed Documents for Biology And Medicine | OSTI...

    Office of Scientific and Technical Information (OSTI)

    April 2013 Most Viewed Documents for Biology And Medicine Publications in biomedical and environmental sciences programs, 1981 Moody, J.B. (comp.) (1982) 306 Drug Retention Times ...

  13. Most Viewed Documents - Biology and Medicine | OSTI, US Dept...

    Office of Scientific and Technical Information (OSTI)

    - Biology and Medicine Drug Retention Times Center for Human Reliability Studies (2007) External dose-rate conversion factors for calculation of dose to the public Not Available ...

  14. June 2015 Most Viewed Documents for Biology And Medicine | OSTI...

    Office of Scientific and Technical Information (OSTI)

    June 2015 Most Viewed Documents for Biology And Medicine Measuring dopamine release in the human brain with PET Volkow, N.D. Brookhaven National Lab., Upton, NY (United ...

  15. Most Viewed Documents for Biology and Medicine: December 2014...

    Office of Scientific and Technical Information (OSTI)

    Most Viewed Documents for Biology and Medicine: December 2014 Dose and volume specification for reporting interstitial therapy NONE (1997) 38 Modification to the Monte Carlo ...

  16. June 2016 Most Viewed Documents for Biology And Medicine | OSTI...

    Office of Scientific and Technical Information (OSTI)

    Biology And Medicine Drug Retention Times Center for Human Reliability Studies (2007) 133 Modification to the Monte Carlo N-Particle (MCNP) Visual Editor (MCNPVised) to Read in ...

  17. July 2013 Most Viewed Documents for Biology And Medicine | OSTI...

    Office of Scientific and Technical Information (OSTI)

    July 2013 Most Viewed Documents for Biology And Medicine Carbon Dioxide Sequestering Using Microalgal Systems Daniel J. Stepan; Richard E. Shockey; Thomas A. Moe; Ryan Dorn (2002) ...

  18. September 2013 Most Viewed Documents for Biology And Medicine...

    Office of Scientific and Technical Information (OSTI)

    September 2013 Most Viewed Documents for Biology And Medicine Drug Retention Times Center for Human Reliability Studies (2007) 29 Oleoresin Capsicum toxicology evaluation and ...

  19. December 2015 Most Viewed Documents for Biology And Medicine...

    Office of Scientific and Technical Information (OSTI)

    December 2015 Most Viewed Documents for Biology And Medicine Measuring dopamine release in the human brain with PET Volkow, N.D. Brookhaven National Lab., Upton, NY (United ...

  20. Functionalized apertures for the detection of chemical and biological materials

    DOEpatents

    Letant, Sonia E.; van Buuren, Anthony W.; Terminello, Louis J.; Thelen, Michael P.; Hope-Weeks, Louisa J.; Hart, Bradley R.

    2010-12-14

    Disclosed are nanometer to micron scale functionalized apertures constructed on a substrate made of glass, carbon, semiconductors or polymeric materials that allow for the real time detection of biological materials or chemical moieties. Many apertures can exist on one substrate allowing for the simultaneous detection of numerous chemical and biological molecules. One embodiment features a macrocyclic ring attached to cross-linkers, wherein the macrocyclic ring has a biological or chemical probe extending through the aperture. Another embodiment achieves functionalization by attaching chemical or biological anchors directly to the walls of the apertures via cross-linkers.

  1. Molecular biology of signal transduction in plants. Abstracts

    SciTech Connect

    Not Available

    1991-12-31

    This volume contains abstracts of oral presentations and poster sessions of the 1991 Cold Springs Harbor Meeting entitled Molecular Biology of Signal Transduction in Plants.

  2. Speciation of Uranium in Biologically Reduced Sediments in the...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Speciation of Uranium in Biologically Reduced Sediments in the Old Rifle Aquifer ... Juan S. Lezama Pacheco The speciation and dynamics of Uranium(IV) in naturally and ...

  3. Biological Conversion of Sugars to Hydrocarbons Technology Pathway...

    Office of Scientific and Technical Information (OSTI)

    This technology pathway case investigates the biological conversion of biomass-derived ... Sponsoring Org: USDOE Office of Energy Efficiency and Renewable Energy Biomass Program ...

  4. Methods for isolation and viability assessment of biological organisms

    DOEpatents

    Letant, Sonia Edith; Baker, Sarah Elyse; Bond, Tiziana; Chang, Allan Shih-Ping

    2015-02-03

    Isolation of biological or chemical organisms can be accomplished using a surface enhanced Raman scattering (SERS) system. The SERS system can be a single or a stacked plurality of photonic crystal membranes with noble-metal lined through pores for flowing analyte potentially containing the biological or chemical organisms. The through pores can be adapted to trap individual biological or chemical organisms and emit SERS spectra, which can then be detected by a detector and further analyzed for viability of the biological or chemical organism.

  5. Linqu Qinchi Biological Co Ltd | Open Energy Information

    OpenEI (Open Energy Information) [EERE & EIA]

    City, Shandong Province, China Zip: 262600 Product: Shandong-based developer of a biogas plant that registered as a CDM project. References: Linqu Qinchi Biological Co.,...

  6. Synthetic biology for microbial production of lipid-based biofuels...

    Office of Scientific and Technical Information (OSTI)

    Synthetic biology for microbial production of lipid-based biofuels Citation Details In-Document Search This content will become publicly available on October 22, 2017 Title: ...

  7. March 2016 Most Viewed Documents for Biology And Medicine | OSTI...

    Office of Scientific and Technical Information (OSTI)

    March 2016 Most Viewed Documents for Biology And Medicine Measuring dopamine release in the human brain with PET Volkow, N.D. Brookhaven National Lab., Upton, NY (United ...

  8. January 2013 Most Viewed Documents for Biology And Medicine ...

    Office of Scientific and Technical Information (OSTI)

    January 2013 Most Viewed Documents for Biology And Medicine Nevada Test Site Radiological Control Manual Radiological Control Managers' Council Nevada Test Site Effects of ionizing ...

  9. Integration of hyperspectral imagery and biosensors for biological...

    Office of Scientific and Technical Information (OSTI)

    facility classification Citation Details In-Document Search Title: Integration of hyperspectral imagery and biosensors for biological and chemical facility classification Authors: ...

  10. Integration of hyperspectral imagery and biosensors for biological...

    Office of Scientific and Technical Information (OSTI)

    facility classification Citation Details In-Document Search Title: Integration of hyperspectral imagery and biosensors for biological and chemical facility classification You ...

  11. Integration of Hyperspectral Imagery and Biosensors for Biological...

    Office of Scientific and Technical Information (OSTI)

    Facility Classification Citation Details In-Document Search Title: Integration of Hyperspectral Imagery and Biosensors for Biological and Chemical Facility Classification You ...

  12. OSTIblog Articles in the Office of Biological and Environmental...

    Office of Scientific and Technical Information (OSTI)

    Biological and Environmental Research (OBER) Abstracts Database Topic Managing the Department of ... on two main areas: the Nation's Energy Security (developing cost-effective ...

  13. Genomics and Systems Biology of Tuberculosis (2009 JGI User Meeting)

    ScienceCinema

    Galagan, James

    2016-07-12

    James Galagan from the Broad Institute spoke about the "Genomics and Systems Biology of TB" on March 26, 2009 during the 4th Annual User Meeting

  14. Enhancing Automatic Biological Pathway Generation with GO-based Gene Similarity

    SciTech Connect

    Sanfilippo, Antonio P.; Baddeley, Robert L.; Beagley, Nathaniel; Riensche, Roderick M.; Gopalan, Banu

    2009-08-03

    One of the greatest challenges in today’s analysis of microarray gene expression data is to identify pathways across regulated genes that underlie structural and functional changes of living cells in specific pathologies. Most current approaches to pathway generation are based on a reverse engineering approach in which pathway plausibility is solely induced from observed pathway data. These approaches tend to lack in generality as they are too dependent on the pathway observables from which they are induced. By contrast, alternative approaches that rely on prior biological knowledge may err in the opposite direction as the prior knowledge is usually not sufficiently tuned to the pathology of focus. In this paper, we present a novel pathway generation approach which combines insights from the reverse engineering and knowledge-based approaches to increase the biological plausibility and specificity of induced regulatory networks.

  15. Method and apparatus for biological sequence comparison

    DOEpatents

    Marr, T.G.; Chang, W.I.

    1997-12-23

    A method and apparatus are disclosed for comparing biological sequences from a known source of sequences, with a subject (query) sequence. The apparatus takes as input a set of target similarity levels (such as evolutionary distances in units of PAM), and finds all fragments of known sequences that are similar to the subject sequence at each target similarity level, and are long enough to be statistically significant. The invention device filters out fragments from the known sequences that are too short, or have a lower average similarity to the subject sequence than is required by each target similarity level. The subject sequence is then compared only to the remaining known sequences to find the best matches. The filtering member divides the subject sequence into overlapping blocks, each block being sufficiently large to contain a minimum-length alignment from a known sequence. For each block, the filter member compares the block with every possible short fragment in the known sequences and determines a best match for each comparison. The determined set of short fragment best matches for the block provide an upper threshold on alignment values. Regions of a certain length from the known sequences that have a mean alignment value upper threshold greater than a target unit score are concatenated to form a union. The current block is compared to the union and provides an indication of best local alignment with the subject sequence. 5 figs.

  16. Method and apparatus for biological sequence comparison

    DOEpatents

    Marr, Thomas G.; Chang, William I-Wei

    1997-01-01

    A method and apparatus for comparing biological sequences from a known source of sequences, with a subject (query) sequence. The apparatus takes as input a set of target similarity levels (such as evolutionary distances in units of PAM), and finds all fragments of known sequences that are similar to the subject sequence at each target similarity level, and are long enough to be statistically significant. The invention device filters out fragments from the known sequences that are too short, or have a lower average similarity to the subject sequence than is required by each target similarity level. The subject sequence is then compared only to the remaining known sequences to find the best matches. The filtering member divides the subject sequence into overlapping blocks, each block being sufficiently large to contain a minimum-length alignment from a known sequence. For each block, the filter member compares the block with every possible short fragment in the known sequences and determines a best match for each comparison. The determined set of short fragment best matches for the block provide an upper threshold on alignment values. Regions of a certain length from the known sequences that have a mean alignment value upper threshold greater than a target unit score are concatenated to form a union. The current block is compared to the union and provides an indication of best local alignment with the subject sequence.

  17. The OME Framework for genome-scale systems biology

    SciTech Connect

    Palsson, Bernhard O.; Ebrahim, Ali; Federowicz, Steve

    2014-12-19

    The life sciences are undergoing continuous and accelerating integration with computational and engineering sciences. The biology that many in the field have been trained on may be hardly recognizable in ten to twenty years. One of the major drivers for this transformation is the blistering pace of advancements in DNA sequencing and synthesis. These advances have resulted in unprecedented amounts of new data, information, and knowledge. Many software tools have been developed to deal with aspects of this transformation and each is sorely needed [1-3]. However, few of these tools have been forced to deal with the full complexity of genome-scale models along with high throughput genome- scale data. This particular situation represents a unique challenge, as it is simultaneously necessary to deal with the vast breadth of genome-scale models and the dizzying depth of high-throughput datasets. It has been observed time and again that as the pace of data generation continues to accelerate, the pace of analysis significantly lags behind [4]. It is also evident that, given the plethora of databases and software efforts [5-12], it is still a significant challenge to work with genome-scale metabolic models, let alone next-generation whole cell models [13-15]. We work at the forefront of model creation and systems scale data generation [16-18]. The OME Framework was borne out of a practical need to enable genome-scale modeling and data analysis under a unified framework to drive the next generation of genome-scale biological models. Here we present the OME Framework. It exists as a set of Python classes. However, we want to emphasize the importance of the underlying design as an addition to the discussions on specifications of a digital cell. A great deal of work and valuable progress has been made by a number of communities [13, 19-24] towards interchange formats and implementations designed to achieve similar goals. While many software tools exist for handling genome

  18. Genomic Functionalization: The Next Revolution In Biology

    SciTech Connect

    Anderson, Peter; Schoeniger, Joseph S.; Imbro, Paula M.

    2014-07-01

    We have implemented a ligand-alignment algorithm into our developed computational pipeline for identifying specificity-determining features (SDFs) in protein-ligand complexes. Given a set of protein-ligand complex structures, the algorithm aligns the complexes by ligand rather than by the C -RMSD or standard approach, providing a single reference frame for extracting SDFs. We anticipate that this ligand-alignment capability will be highly useful for protein function prediction. We already have a database containing > 20 K ligand-protein complex crystal structures taken from the Protein Data Bank. By aligning these proteins to single reference frames using ligand alignment, we can submit the complexes to our pipeline for SDF extraction. The SDFs derived from this training procedure can be used as thumbprints that are hallmarks of individual enzyme classes. These SDF thumbprints may then serve as guides to the prediction of function of new unknown proteins.

  19. IBEX satellite finds ribbon-like structure at edge of heliosphere

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    IBEX satellite IBEX satellite finds ribbon-like structure at edge of heliosphere The NASA IBEX mission has sent back data that indicates a "noodle soup" of solar material has accumulated at the outer fringes of the heliosphere bubble. October 15, 2009 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy sources, to plasma

  20. Structural Health Monitoring

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Structural Health Monitoring Engineering Institute Structural Health Monitoring Structural Health Monitoring is the process of implementing a damage detection strategy for...

  1. Stormwater Control Structures

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Stormwater Control Structures Stormwater Control Structures Stormwater control structures are engineered to control run-on and runoff water from suspected contaminated sites. ...

  2. 2013 Biological Hydrogen Production Workshop Summary Report

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    (Photos from top to bottom) Cultures of green algae producing hydrogen from water and light. Photo courtesy of National Renewable Energy Laboratory (NREL). (NREL 14579) A model of the small subunit of a hydrogenase enzyme, showing the metal clusters as colored balls and the protein structure as green ribbons. The amino acids in red indicate substitutions that improved hydrogen evolution rates. Photo courtesy of Philip D. Weyman, J. Craig Venter Institute Bacteria break down biomass to produce

  3. Reversibly immobilized biological materials in monolayer films on electrodes

    DOEpatents

    Weaver, P.F.; Frank, A.J.

    1993-05-04

    Methods and techniques are described for reversibly binding charged biological particles in a fluid medium to an electrode surface. The methods are useful in a variety of applications. The biological materials may include microbes, proteins, and viruses. The electrode surface may consist of reversibly electroactive materials such as polyvinylferrocene, silicon-linked ferrocene or quinone.

  4. Reversibly immobilized biological materials in monolayer films on electrodes

    DOEpatents

    Weaver, Paul F. (Golden, CO); Frank, Arthur J. (Lakewood, CO)

    1993-01-01

    Methods and techniques are described for reversibly binding charged biological particles in a fluid medium to an electrode surface. The methods are useful in a variety of applications. The biological materials may include microbes, proteins, and viruses. The electrode surface may consist of reversibly electroactive materials such as polyvinylferrocene, silicon-linked ferrocene or quinone.

  5. Management of Biological Materials in Wastewater from Research & Development Facilities

    SciTech Connect

    Raney, Elizabeth A.; Moon, Thomas W.; Ballinger, Marcel Y.

    2011-04-01

    PNNL has developed and instituted a systematic approach to managing work with biological material that begins in the project planning phase and carries through implementation to waste disposal. This paper describes two major processes used at PNNL to analyze and mitigate the hazards associated with working with biological materials and evaluate them for disposal to the sewer, ground, or surface water in a manner that protects human health and the environment. The first of these processes is the Biological Work Permit which is used to identify requirements for handling, storing, and working with biological materials and the second is the Sewer Approval process which is used to evaluate discharges of wastewaters containing biological materials to assure they meet industrial wastewater permits and other environmental regulations and requirements.

  6. Computational Structural Mechanics

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    load-2 TRACC RESEARCH Computational Fluid Dynamics Computational Structural Mechanics Transportation Systems Modeling Computational Structural Mechanics Overview of CSM ...

  7. Magnetic multilayer structure

    DOEpatents

    Herget, Philipp; O'Sullivan, Eugene J.; Romankiw, Lubomyr T.; Wang, Naigang; Webb, Bucknell C.

    2016-07-05

    A mechanism is provided for an integrated laminated magnetic device. A substrate and a multilayer stack structure form the device. The multilayer stack structure includes alternating magnetic layers and diode structures formed on the substrate. Each magnetic layer in the multilayer stack structure is separated from another magnetic layer in the multilayer stack structure by a diode structure.

  8. Molecular biology of Homo sapiens: Abstracts of papers presented at the 51st Cold Spring Harbor symposium on quantitative biology

    SciTech Connect

    Watson, J.D.; Siniscalco, M.

    1986-01-01

    This volume contains abstracts of papers presented at the 51st Cold Springs Harbor Symposium on Quantitative Biology. The topic for this meeting was the ''Molecular Biology of Homo sapiens.'' Sessions were entitled Human Gene Map, Human Cancer Genes, Genetic Diagnosis, Human Evolution, Drugs Made Off Human Genes, Receptors, and Gene Therapy. (DT)

  9. A New Biology for the 21st Century; Ensuring the United States Leads the Coming Biology Revolution. Final committee report

    SciTech Connect

    None None

    2012-05-10

    In July, 2008, the National Institutes of Health (NIH), National Science Foundation (NSF), and Department of Energy (DOE) asked the National Research Council’s Board on Life Sciences to convene a committee to examine the current state of biological research in the United States and recommend how best to capitalize on recent technological and scientific advances that have allowed biologists to integrate biological research findings, collect and interpret vastly increased amounts of data, and predict the behavior of complex biological systems. From September 2008 through July of 2009, a committee of 16 experts from the fields of biology, engineering and computational science undertook to delineate those scientific and technological advances and come to a consensus on how the U.S. might best capitalize on them. This report, authored by the Committee on a New Biology for the 21st Century, describes the committee’s work and conclusions.

  10. Systems Biology Knowledgebase for a New Era in Biology A Genomics:GTL Report from the May 2008 Workshop

    SciTech Connect

    Gregurick, S.; Fredrickson, J. K.; Stevens, R.

    2009-03-01

    Biology has entered a systems-science era with the goal to establish a predictive understanding of the mechanisms of cellular function and the interactions of biological systems with their environment and with each other. Vast amounts of data on the composition, physiology, and function of complex biological systems and their natural environments are emerging from new analytical technologies. Effectively exploiting these data requires developing a new generation of capabilities for analyzing and managing the information. By revealing the core principles and processes conserved in collective genomes across all biology and by enabling insights into the interplay between an organism's genotype and its environment, systems biology will allow scientific breakthroughs in our ability to project behaviors of natural systems and to manipulate and engineer managed systems. These breakthroughs will benefit Department of Energy (DOE) missions in energy security, climate protection, and environmental remediation.

  11. Mesos-scale modeling of irradiation in pressurized water reactor concrete biological shields

    SciTech Connect

    Le Pape, Yann; Huang, Hai

    2016-01-01

    Neutron irradiation exposure causes aggregate expansion, namely radiation-induced volumetric expansion (RIVE). The structural significance of RIVE on a portion of a prototypical pressurized water reactor (PWR) concrete biological shield (CBS) is investigated by using a meso- scale nonlinear concrete model with inputs from an irradiation transport code and a coupled moisture transport-heat transfer code. RIVE-induced severe cracking onset appears to be triggered by the ini- tial shrinkage-induced cracking and propagates to a depth of > 10 cm at extended operation of 80 years. Relaxation of the cement paste stresses results in delaying the crack propagation by about 10 years.

  12. Biologically based multistage modeling of radiation effects

    SciTech Connect

    William Hazelton; Suresh Moolgavkar; E. Georg Luebeck

    2005-08-30

    This past year we have made substantial progress in modeling the contribution of homeostatic regulation to low-dose radiation effects and carcinogenesis. We have worked to refine and apply our multistage carcinogenesis models to explicitly incorporate cell cycle states, simple and complex damage, checkpoint delay, slow and fast repair, differentiation, and apoptosis to study the effects of low-dose ionizing radiation in mouse intestinal crypts, as well as in other tissues. We have one paper accepted for publication in ''Advances in Space Research'', and another manuscript in preparation describing this work. I also wrote a chapter describing our combined cell-cycle and multistage carcinogenesis model that will be published in a book on stochastic carcinogenesis models edited by Wei-Yuan Tan. In addition, we organized and held a workshop on ''Biologically Based Modeling of Human Health Effects of Low dose Ionizing Radiation'', July 28-29, 2005 at Fred Hutchinson Cancer Research Center in Seattle, Washington. We had over 20 participants, including Mary Helen Barcellos-Hoff as keynote speaker, talks by most of the low-dose modelers in the DOE low-dose program, experimentalists including Les Redpath (and Mary Helen), Noelle Metting from DOE, and Tony Brooks. It appears that homeostatic regulation may be central to understanding low-dose radiation phenomena. The primary effects of ionizing radiation (IR) are cell killing, delayed cell cycling, and induction of mutations. However, homeostatic regulation causes cells that are killed or damaged by IR to eventually be replaced. Cells with an initiating mutation may have a replacement advantage, leading to clonal expansion of these initiated cells. Thus we have focused particularly on modeling effects that disturb homeostatic regulation as early steps in the carcinogenic process. There are two primary considerations that support our focus on homeostatic regulation. First, a number of epidemiologic studies using multistage

  13. SU-E-I-75: Development of New Biological Fingerprints for Patient Recognition to Identify Misfiled Images in a PACS Server

    SciTech Connect

    Shimizu, Y; Yoon, Y; Iwase, K; Yasumatsu, S; Matsunobu, Y; Morishita, J

    2015-06-15

    Purpose: We are trying to develop an image-searching technique to identify misfiled images in a picture archiving and communication system (PACS) server by using five biological fingerprints: the whole lung field, cardiac shadow, superior mediastinum, lung apex, and right lower lung. Each biological fingerprint in a chest radiograph includes distinctive anatomical structures to identify misfiled images. The whole lung field was less effective for evaluating the similarity between two images than the other biological fingerprints. This was mainly due to the variation in the positioning for chest radiographs. The purpose of this study is to develop new biological fingerprints that could reduce influence of differences in the positioning for chest radiography. Methods: Two hundred patients were selected randomly from our database (36,212 patients). These patients had two images each (current and previous images). Current images were used as the misfiled images in this study. A circumscribed rectangular area of the lung and the upper half of the rectangle were selected automatically as new biological fingerprints. These biological fingerprints were matched to all previous images in the database. The degrees of similarity between the two images were calculated for the same and different patients. The usefulness of new the biological fingerprints for automated patient recognition was examined in terms of receiver operating characteristic (ROC) analysis. Results: Area under the ROC curves (AUCs) for the circumscribed rectangle of the lung, upper half of the rectangle, and whole lung field were 0.980, 0.994, and 0.950, respectively. The new biological fingerprints showed better performance in identifying the patients correctly than the whole lung field. Conclusion: We have developed new biological fingerprints: circumscribed rectangle of the lung and upper half of the rectangle. These new biological fingerprints would be useful for automated patient identification system

  14. Kinetic model for anaerobic digestion of biogas biological sludge

    SciTech Connect

    Pavlostathis, S.G.; Gossett, J.M.

    1986-10-01

    The principal objective of this study was the development and evaluation of a comprehensive kinetic model capable of predicting digester performance when fed biological sludge. Preliminary conversion mechanisms such as cell deaths, lysis, and hydrolysis responsible for rendering viable biological sludge organisms to available substrate were studied in depth. The results of this study indicate that hydrolysis of the dead, particulate biomass - primarily consisting of protein - is the slowest step, and therefore kinetically controls the overall process of an anaerobic digestion of biological sludge. A kinetic model was developed which could accurately describe digester performance and predict effluent quality.

  15. Programmed assembly of nanoscale structures using peptoids.

    SciTech Connect

    Ren, Jianhua; Russell, Scott; Morishetti, Kiran; Robinson, David B.; Zuckermann, Ronald N.; Buffleben, George M.; Hjelm, Rex P.; Kent, Michael Stuart

    2011-02-01

    Sequence-specific polymers are the basis of the most promising approaches to bottom-up programmed assembly of nanoscale materials. Examples include artificial peptides and nucleic acids. Another class is oligo(N-functional glycine)s, also known as peptoids, which permit greater sidegroup diversity and conformational control, and can be easier to synthesize and purify. We have developed a set of peptoids that can be used to make inorganic nanoparticles more compatible with biological sequence-specific polymers so that they can be incorporated into nucleic acid or other biologically based nanostructures. Peptoids offer degrees of modularity, versatility, and predictability that equal or exceed other sequence-specific polymers, allowing for rational design of oligomers for a specific purpose. This degree of control will be essential to the development of arbitrarily designed nanoscale structures.

  16. Microsoft Word - DOE GTL grant - biological hydrogen production...

    Office of Scientific and Technical Information (OSTI)

    ... PLoS Computational Biology (2010), 6(11): e1001002. Miriam A. Rosenbaum, Haim Y. Bar, Qasim K. Beg, Daniel Segre, James Booth, Michael Cotta, Largus T. Angenent: Transcriptional ...

  17. Biological Imaging by Soft X-Ray Diffraction Microscopy

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in...

  18. EA-2011: Proposed Release of Three Parasitoids for the Biological...

    Energy.gov [DOE] (indexed site)

    the continental U.S. for the biological control of the emerald ash borer, a nonnative invasive beetle. The DOE Oak Ridge Office reviewed the EA, adopted it, and issued a FONSI for...

  19. June 2014 Most Viewed Documents for Biology And Medicine | OSTI...

    Office of Scientific and Technical Information (OSTI)

    June 2014 Most Viewed Documents for Biology And Medicine Modification to the Monte Carlo N-Particle (MCNP) Visual Editor (MCNPVised) to Read in Computer Aided Design (CAD) Files ...

  20. Biology Chemistry & Material Science Laboratory 1 | Sample Preparation...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    1 Cynthia Patty | (650) 926-3925 Biology Chemistry & Material Science Laboratory 1 Inventory The BioChemMat Lab 1 at SSRL is dedicated to researcher experiments, including x-ray ...

  1. John Marohn > ProfessorChemistry and Chemical Biology > Faculty...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Marohn Professor Chemistry and Chemical Biology Research Group Webpage jam99@cornell.edu John Marohn earned a B.S. in Chemistry and a B.A. in Physics from the University of ...

  2. March 2015 Most Viewed Documents for Biology And Medicine | OSTI...

    Office of Scientific and Technical Information (OSTI)

    5 Most Viewed Documents for Biology And Medicine Measuring dopamine release in the human brain with PET Volkow, N.D. Brookhaven National Lab., Upton, NY (United States)|State ...

  3. Biology Chemistry & Material Science Laboratory 2 | Sample Preparation...

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    2 Cynthia Patty | (650) 926-3925 Biology Chemistry & Material Science Laboratory 2 Inventory The BioChemMat Lab 2 (BCM 2) at SSRL is dedicated to researcher experiments, including ...

  4. Methods of increasing secretion of polypeptides having biological activity

    DOEpatents

    Merino, Sandra

    2015-04-14

    The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

  5. Methods of increasing secretion of polypeptides having biological activity

    DOEpatents

    Merino, Sandra

    2014-05-27

    The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

  6. Methods of increasing secretion of polypeptides having biological activity

    DOEpatents

    Merino, Sandra

    2013-10-01

    The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

  7. Methods of increasing secretion of polypeptides having biological activity

    DOEpatents

    Merino, Sandra

    2014-10-28

    The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

  8. Biological assessments for the low energy demonstration accelerator, 1996

    SciTech Connect

    Cross, S.

    1997-03-01

    This report discusses the biological impact to the area around the Los Alamos National Laboratory of the Low Energy Demonstration Accelerator. In particular the impact to the soils, water quality, vegetation, and wildlife are discussed.

  9. Biological Imaging by Soft X-Ray Diffraction Microscopy

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in ...

  10. Investigations of biological processes in Austrian MBT plants

    SciTech Connect

    Tintner, J.; Smidt, E.; Boehm, K.; Binner, E.

    2010-10-15

    Mechanical biological treatment (MBT) of municipal solid waste (MSW) has become an important technology in waste management during the last decade. The paper compiles investigations of mechanical biological processes in Austrian MBT plants. Samples from all plants representing different stages of degradation were included in this study. The range of the relevant parameters characterizing the materials and their behavior, e.g. total organic carbon, total nitrogen, respiration activity and gas generation sum, was determined. The evolution of total carbon and nitrogen containing compounds was compared and related to process operation. The respiration activity decreases in most of the plants by about 90% of the initial values whereas the ammonium release is still ongoing at the end of the biological treatment. If the biogenic waste fraction is not separated, it favors humification in MBT materials that is not observed to such extent in MSW. The amount of organic carbon is about 15% dry matter at the end of the biological treatment.

  11. Arms Control: US and International efforts to ban biological weapons

    SciTech Connect

    Not Available

    1992-12-01

    The Bacteriological (Biological) and Toxin Weapons Convention, the treaty that bans the development, production, and stockpiling and acquisition of biological weapons was opened for signature in 1972 and came into force in 1975 after being ratified by 22 governments, including the depository nations of the USA, the United Kingdom, and the former Soviet Union. In support of the Convention, the USA later established export controls on items used to make biological weapons. Further, in accordance with the 1990 President`s Enhanced Proliferation Control Initiative, actions were taken to redefine and expand US export controls, as well as to encourage multilateral controls through the Australia Group. Thus far, the Convention has not been effective in stopping the development of biological weapons. The principal findings as to the reasons of the failures of the Convention are found to be: the Convention lacks universality, compliance measures are effective, advantage of verification may outweigh disadvantages. Recommendations for mitigating these failures are outlined in this report.

  12. BIOISIS: Biological Macromolecules by Small Angle X-ray Scattering (SAXS)

    DOE Data Explorer

    Tainer, John [Scripps Research Institute; Hura, Greg [LBNL; Rambo, Robert P. [LBNL

    BIOISIS is an open access database dedicated to the study of biological macromolecules by small angle X-ray scattering (SAXS). BIOISIS aims to become the complete source for the deposition, distribution and maintenance of small angle X-ray scattering data and technologies. The database is designed around the concept of an ôexperimentö and relates a specific experiment to a set of genes, organisms, computational models and experimental data. As of May 2012, BIOSIS contains 7,118 genes covering four different organisms. Forty-two modeled structures are available. Clicking on a structures reveals scattering curves, experimental conditions, and experimental values. The data are collected at Beamline 12.3.1 of the Advanced Light Source (ALS).[Copied with editing from http://www.bioisis.net/about

  13. BIOISIS: Biological Macromolecules by Small Angle X-ray Scattering (SAXS)

    DOE Data Explorer

    Tainer, John [Scripps Research Institute; Hura, Greg [LBNL; Rambo, Robert P. [LBNL

    BIOISIS is an open access database dedicated to the study of biological macromolecules by small angle X-ray scattering (SAXS). BIOISIS aims to become the complete source for the deposition, distribution and maintenance of small angle X-ray scattering data and technologies. The database is designed around the concept of an experiment and relates a specific experiment to a set of genes, organisms, computational models and experimental data. As of May 2012, BIOSIS contains 7,118 genes covering four different organisms. Forty-two modeled structures are available. Clicking on a structures reveals scattering curves, experimental conditions, and experimental values. The data are collected at Beamline 12.3.1 of the Advanced Light Source (ALS).[Copied with editing from http://www.bioisis.net/about

  14. Measuring Real-time Biological and Abiotic Manganese Oxide Reduction |

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Stanford Synchrotron Radiation Lightsource Measuring Real-time Biological and Abiotic Manganese Oxide Reduction Tuesday, May 31, 2016 Manganese(IV) oxides are powerful scavengers of toxins and trace metals, but they are also strong oxidants in the environment (1). Certain common microbes can also 'breathe' manganese oxides, in a process known as anaerobic respiration (2). During these environmental -commonly with sulfur or iron species- and biological interactions, manganese oxides are often

  15. Division of Chemical & Biological Sciences | The Ames Laboratory

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Chemical & Biological Sciences [PHOTO]Theory and modeling Researchers from the Ames Laboratory's Division of Chemical and Biological Sciences are developing computational tools to understand diffusion and reactivity in porous materials. Read more [PHOTO]Dynamic Nuclear Polarization The Ames Laboratory is home to the United States' first dynamic nuclear polarization (DNP) solid-state nuclear magnetic resonance (NMR) spectrometer dedicated to the study of materials science and chemistry. Read

  16. Large Scale Production Computing and Storage Requirements for Biological

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    and Environmental Research: Target 2017 Large Scale Production Computing and Storage Requirements for Biological and Environmental Research: Target 2017 BERmontage.gif September 11-12, 2012 Hilton Rockville Hotel and Executive Meeting Center 1750 Rockville Pike Rockville, MD, 20852-1699 TEL: 1-301-468-1100 Sponsored by: U.S. Department of Energy Office of Science Office of Advanced Scientific Computing Research (ASCR) Office of Biological and Environmental Research (BER) National Energy

  17. Flow cytometry aids basic cell biology research and drug discovery

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Flow cytometry aids basic cell biology research and drug discovery Flow cytometry aids basic cell biology research and drug discovery Life Technologies Corporation and LANL have released the Attune® Acoustic Focusing Cytometer, featuring a reduced footprint, reduced consumables, and an affordable price. April 3, 2012 Attune® Acoustic Focusing Cytometer The Attune® Acoustic Focusing Cytometer achieves sample throughput at rates over 10 times faster than other cytometers-up to 1,000 μL per

  18. Biological Conversion of Sugars to Hydrocarbons Technology Pathway |

    Office of Energy Efficiency and Renewable Energy (EERE) (indexed site)

    Department of Energy to Hydrocarbons Technology Pathway Biological Conversion of Sugars to Hydrocarbons Technology Pathway This technology pathway case investigates the biological conversion of biomass-derived sugars to hydrocarbon biofuels, utilizing data from recent literature references and information consistent with recent pilot-scale demonstrations at NREL. Technical barriers and key research needs have been identified that should be pursued for the pathway to become competitive with

  19. A Systems Biology Platform for Characterizing Regulatory and Metabolic

    Office of Scientific and Technical Information (OSTI)

    Pathways that Influence and Control Microbial Hydrogen Production (Technical Report) | SciTech Connect A Systems Biology Platform for Characterizing Regulatory and Metabolic Pathways that Influence and Control Microbial Hydrogen Production Citation Details In-Document Search Title: A Systems Biology Platform for Characterizing Regulatory and Metabolic Pathways that Influence and Control Microbial Hydrogen Production Authors: Collins, James J [1] + Show Author Affiliations Boston University

  20. Computational Biology: A Recipe for Ligand-Binding Proteins

    U.S. Department of Energy (DOE) - all webpages (Extended Search)

    Computational Biology: A Recipe for Ligand-Binding Proteins Authors: Ghirlanda, G. Title: Computational Biology: A Recipe for Ligand-Binding Proteins Source: Nature Year: 2013 Volume: 501 Pages: 177-178 ABSTRACT: Cellular cross-talk, enzymatic catalysis and regulation of gene expression all depend on molecular recognition. A method that allows the design of proteins with desired recognition sites could thus be revolutionary Date of online publication: Thu, 2013-09-12 Link online: